Interactions amongst Grain Variables in Various Dwarf Dry Bean (Phaseolus vulgaris L.) Cultivars

Common bean ( Phaseolus vulgaris L.) is an important leguminous crop. Seed variables such as protein, oil, cellulose, ash, nitrogen-free extract, testa and moisture contents and the thousand-seed weights of seven bean cultivars currently cultivated in Turkey, namely, A-111 Pinto, Çalı, Yunus-90, Eskişehir-855, Şehirali-90, Karacaşehir-90 and Romano, were analysed in the laboratory with respect to their effects on the quality of bean seed grown under Central Anatolian ecological conditions. Statistically significant variations were found by ANOVA amongst the cultivars for all the variables examined, except the ash content of the seeds, and different groups were obtained by Duncan's multiple range test. Correlations amongst the variables as well as their direct and indirect effects on protein content were also calculated using the correlation and path coefficients analyses, respectively.     

Yield Formation in Mixtures of Rust Resistant and Susceptible Plants of Common Bean (Phaseolus vulgaris L.)

In a two-factorial trial, conducted in 1986 at the CIAT farm in Palmira (Colombia), the performance of nine tropical bean lines differing in resistance to rust and seven mixtures obtained by combining these lines on the basis of equal proportions in seed number was evaluated in a rust inoculated treatment and in a fungicide treated control.
Compared to the fungicide treated control, rust attack significantly reduced the yield of all susceptible lines by 24 to 56% in pure stands. In rust inoculated mixtures containing resistant, intermediate and susceptible genotypes, mixture effects on yield increased only by 3.3% compared to the fungicide treated control. In mixtures of common bean a higher proportion of resistant lines appears to be required compared to cereals for comparable reductions in the spread of rust due to lower plant density and correspondingly higher autodeposition rate of uredospores.     

Effects of Deficit Irrigation and Salinity Stress on Common Bean (Phaseolus Vulgaris L.) and Mungbean (Vigna Radiata (L.) Wilczek) Grown in a Controlled Environment

As water for irrigation purposes becomes increasingly scarce because of climate change and population growth, there is growing interest in regulated deficit irrigation (RDI) as a way to improve efficiency of water usage and farm productivity in arid and semi‐arid areas. Salinity is also becoming an important problem in these same regions. Experiments were performed to investigate the effects of RDI and salt stress on two legumes crops, common bean (Phaseolus vulgaris L.) and mungbean (Vigna radiata (L.) Wilczek); previous work showed contrasting responses to RDI by these two crops under field conditions. The seed and biomass yields of both crops were reduced as a result of increasing water deficit stress; however, mungbean was able to maintain the same proportion of its biomass in reproductive structures and maintain its harvest index under stress, whereas common bean’s decreased. In addition, photosynthesis in mungbean was higher than in common bean and higher at the same levels of transpiration. Finally, salinity stress did not affect the water potential, harvest index or the specific leaf weight of either crop. There were no interactions between salinity and crops or RDI levels, which suggest that the two crops do not differ in their response to salinity stress, and that RDI levels do not modify this response.     

Effects of Interactions of Moisture Regime and Nutrient Addition on Nodulation and Carbon Partitioning in Two Cultivars of Bean (Phaseolus vulgaris L.)

Major limitations of bean ( Phaseolus vulgaris L.) production in arid and semiarid regions are lack of moisture and low soil fertility. An experiment was conducted to determine the effects of soil moisture and N : P : K (20 : 10 : 10) fertilizer on root and shoot growth of two cultivars of bean: cv. Carioca, an indeterminate Brazilian landrace, and cv. Prince, a determinate cultivar grown in Europe. Carioca appears generally stress-tolerant while Prince is intolerant. Seedlings were grown in pots of non-sterile soil at 30, 60 or 90 % field capacity (FC), and given 0, 0.1 or 1 g (kg soil)^–1 of compound fertilizer. The soil contained a population of effective Rhizobium . Growth of both cultivars was greatest in the high moisture and high nutrient treatments. Root fractions were highest at low nutrient supply; the effect of water was not significant. Leaf fraction decreased as root fraction increased. Numbers of nodules were highest at high and intermediate moisture when no fertilizer was applied. Numbers were lowest at 30 % FC and at the highest fertilizer rate. Masses of nodules and fractions followed the same pattern. Decreasing water regime reduced the relative growth rate (RGR) of Prince, while Carioca maintained high RGR at unfavourable conditions of water and nutrients. Net assimilation rates (NAR) were unaffected by nutrient addition, and reduced by low moisture regime. Water use efficiencies (WUEs) were reduced by water stress but increased by nutrient deficiency. The water utilization for dry matter production was optimal at 60 % FC.     

Yield stability in common bean (Phaseolus vulgaris L.) genotypes

An experiment was undertaken to determine the stability of seed yield in 21 common bean genotypes representing three growth habits. Seven genotypes in each growth habit (determinate bush, indeterminate bush and indeterminate prostrate) were evaluated in replicated trials at three locations for three years under rain fed conditions in Ethiopia. A combined analysis of variance, stability statistics and rank correlations among stability statistics and yield-stability statistic were determined. The genotypes differed significantly for seed yield and genotype × environment (year by location) interaction (GE). The different stability statistics namely Type1, Type 2 and Type 3 measured the different aspects of stability. This was substantiated by rank correlation coefficient. There were strong rank correlations among S_i ²d, W_i ²,σ_i ² and S_i ², where as there was weak correlation between b_iand R_i ², S_i ²d, W_i ², σ_i ² and S_i ². R² was significantly and negatively correlated with W_i ², σ_i ² and S_i ². σ_i ² is significantly correlated with W_i ².Yield is significantly correlated with b_i and R_i ².None of the statistics per se was useful for selecting high yielding and stable genotypes except the YS(yield-stability statistic). Most of the high yielding genotypes were relatively stable. Of the 21 genotypes, only 11genotypes were selected for their high yielding and stable performance. Genotypes with growth habit III and I (in determinate prostrate and determinate bush) were generally more stable than in determinate bush. This revised version was published online in July 2006 with corrections to the Cover Date.     

普通菜豆抗旱生理特性

采用盆栽试验,以抗旱性较好的品种跃进豆、260205和敏感品种奶花芸豆为试材,设置干旱和正常供水2种处理,测定产量、产量构成因素及相关生理生化指标,分析干旱胁迫下参试品种各性状及生理指标的变化及对干旱胁迫的生理响应。结果表明,干旱处理36 d,跃进豆和260205的根干重为总生物量的20.2%和20.6%,荚干重为总生物量的30.0% 和28.9%,而奶花芸豆的根干重和荚干重仅为总生物量的10.6%和17.1%,光合产物向根系和籽粒的有效分配与普通菜豆抗旱性关系密切;跃进豆在干旱胁迫后期的水分利用效率较对照增加 230.5%,而奶花芸豆的增幅仅为84.3%,较高的水分利用效率有利于CO₂的有效扩散和高效固定;其他生理特性分析表明,抗氧化酶与光呼吸共同作用有效降低了膜脂过氧化程度,减少了叶片的损伤;脯氨酸和可溶性糖是普通菜豆主要的渗透调节物质,能够较好地保持自身叶片的水分平衡。普通菜豆抗旱性是多种生理调节机制协同作用的结果,主要包括形态调节、气孔调节、渗透调节以及抗氧化能力的调节等。     

间作和双接种对玉米和菜豆生长的影响

为了验证菜豆玉米间作的可行性及双接种丛枝菌根真菌和根瘤菌在此间作体系中的影响。通过采用盆栽试验的方式,测定了不同栽培及接种方式下玉米和菜豆的一些生理指标如：生物量、氮磷钾元素的含量及吸收量、叶绿素SPAD值、菌根侵染率等。结果表明：单接种时,单作下的玉米和菜豆的总生物量分别是间作下的3.26 倍和1.25 倍;双接种时也分别达到了3.08 倍和1.25 倍。然而单接种时玉米和菜豆间作后每盆的吸氮量和吸磷量分别是间作下的1.11 倍和1.05 倍;双接种时则均为1.16 倍。接种丛枝菌根真菌后玉米及菜豆的生物量、氮磷钾元素的含量及吸收量、叶绿素含量等差异均不显著,而菜豆的根瘤数却降低了。试验研究表明：间作抑制了菜豆及玉米的生长,但促进了二者对氮磷元素的吸收利用;接种丛枝菌根真菌对玉米及菜的生长无促进作用,对二者吸收利用氮磷钾元素也无明显促进,而且还抑制了根瘤菌侵染菜豆。     

Male sterility in beans (Phaseolus vulgaris L.)

Summary Male sterile bean plants were discovered at Embu Agricultural Research Station, Kenya. The character is controlled by one recessive gene.The Grain Legume Project is a joint project of the Kenya Ministry of Agriculture and The Netherlands Ministry of Foreign Affairs.     

The Influence of Plant Water Stress on Net Photosynthesis and Leaf Area of Two Maize (Zea mays L.) Cultivars

The effect of plant water stress on net photosynthesis and leaf growth were investigated in order to determine to what extent leaf water potential during vegetative growth and silking affects maize development.
Two commercial maize hybrids grown in pots in a glasshouse were subjected to leaf water potentials of -1300 and -1700 kPa during the eighth leaf stage and during silking to -1700 and -2300 kPa to previously unstressed, moderately and severely stressed plants. The effect of stress on inhibiting CO₂ uptake rates and leaf areas, as well as the recovery after alleviating stress, were compared to that of unstressed plants.
No substantial differences in CO₂ uptake rates were found between medium and long seasoned cultivars. The CO₂ uptake rates per unit leaf area decreased to negative values under both moderate and severe stress conditions during both growth stages. During silking, the recovery of CO₂ uptake rate was much lower than during the eight leaf stage. Leaf area decreased proportionally with increased stress but did not recover after alleviating stress on plants stressed during both the eighth leaf and silking stages.     

Growth analysis in a reduced leaf mutant of common bean (Phaseolus vulgaris L.)

Summary A comparison between the Royal Red bean variety and a reduced leaf mutant on the basis of growth indices is reported. The mutants has lower leaf area index (LAI) and leaf area duration (LAD) (–43.7%), higher specific leaf weight (SLW) (on the mean +33.3%) and accumulates less dry matter (–7.7%) than the normal genotype. When the net assimilation rate (NAR) is considered, the mutant is superior to the normal (+62.3%). It is concluded that the unit area of the mutant leaves accumulates dry matter more efficiently than that of the corresponding normal variety.     

Genetics of Seed Yield and its Components in Common Bean (Phaseolus vulgaris L.) of Middle-American Origin

Genetic variance, heritability, and expected response from selection arc useful in devising alternative methods and criteria of: selection. The objectives of this study were to estimate these for seed yield and its components from 200 F₂: populations involving 80 cultivars and lines of mostly small-seeded dry bush bean (Phaseolus vulgaris L.) of habits growth I, II, and III of Middle-American origin. All cultivars and lines were crossed in eight sets of ten parents each in a Design II mating system. The F₂ populations, without parents, were evaluated in the field in a replicates-in-sets design at two locations in Colombia in 1983. Estimates of additive genetic variance were significant for yield, pods/m², seeds/pod, and seed weight. Interaction with environments was also significant. Values for nonadditive genetic variance were not significant for either yield or yield components. The estimates of narrow sense heritability, based on the F₂ population mean and unbiased by genotype x environment interaction, were 0.21 ± 0.13 for yield. 20 ± 0.13 for pods/m², 0.57 ± 0.13 for seeds/pod, and 0.74 ± 0.15 for seed weight. The expected direct response from selection of the top 20 % of F₂ populations for yield per se would result in a 4.30 % increase in yield with a correlated response of 0.21 % in seed weight. In contrast, the expected gain from direct selection for seed weight would result in a 11.76 % increase in seed weight with a, correlated gain of 0.28 % for yield. Direct selection for pods/m² would decrease yield, seeds/pod and seed weight, while direct selection for seeds/pod would reduce pods/m² and seed weight but increase seed yield by 0.37 %. Data on yield from replicated trials in the early segregating generations could be utilized for identification and selection of promising crosses and families or lines with crosses for dry bean yield improvement.     

The Influence of Plant Water Stress on Net Photosynthesis and Yield of Sunflower (Helianthus annuus L.)

The effect of plant water stress on net photosynthesis, leaf growth, yield and yield-related components were investigated in a single experiment in order to determine in which way water deficits affect sunflower yields.
Sunflower plants, grown under controlled temperature regimes, were stressed during budding, anthesis and seed filling by withholding water until the leaf water potential reached -1600 and -2000 kPa. Leaf area of unstressed plants significantly exceeded that of plants under severe stress during all growth stages investigated. The CO₂ uptake rate per unit leaf area as well as the total uptake rate per plant, significantly diminished with stress, while this effect drastically increased during the reproductive phase of the plant. Although this resulted in significantly smaller heads and kernels, it did not affect the number of seeds borne in the inflorescence. Severe stress during anthesis and seed filling resulted in more empty kernels. Moderate and severe stress during budding significantly lowered both grain and oil yields while plants that experienced moderate stress during anthesis and seed filling significantly outyielded those under severe stress.     

水分胁迫对玉米生长发育及产量形成的影响研究

采用人工控制水分的方法,研究玉米生长发育和产量形成对水分胁迫的响应。研究结果表明：干旱胁迫导致玉米生长发育缓慢和减产程度的大小,因胁迫时期、胁迫程度及持续时间而不同。干旱胁迫对株高的抑制作用：拔节孕穗期>抽雄吐丝期>苗期,其中苗期株高在复水后得到了超补偿。受水分胁迫影响穗重、穗粒重和穗粒数都呈减少的趋势,变化幅度为穗粒数>穗重>穗粒重,不同生育期干旱胁迫处理的减产幅度为抽雄吐丝期>拔节孕穗期>苗期。苗期、拔节孕穗期和抽穗开花期减产程度分别达到30%、70%和90%以上。     

Traits associated with dry edible bean (Phaseolus vulgaris L.) productivity under diverse soil moisture environments

Two experiments were conducted in the Rift Valley, Ethiopia (8°N and 39°E) to determine associations between eight plant traits and seed yield, and to obtain estimates of narrow sense heritability for the traits. Experiment I evaluated seven dry edible bean cultivars/lines at two locations to simulate different soil moisture stress, including, Debre Zeit(non-stress) and Dera (moderate-stress). Experiment II evaluated 25 cultivars/lines in three environments including, Melkassa early planted (non-stress), Melkassa late planted (high-stress), and Dera (moderate-stress). A randomized-complete-block design with three replicates was used in both experiments. Plant traits evaluated were seed yield, pods plant^-1, seeds pod^-1, 100 seed weight, root dry weight, hypocotyl diameter, plant biomass, plant height and days to flowering. Plant traits that were significantly associated with seed yield were included in a stepwise-regression model to determine which trait or combination of traits provided the best model to estimate seed yield in each environment. An analysis of variance was conducted to test main effects and interactions between plant traits and environments. Significant variation among lines occurred for seed yield and all plant traits in both experiments. Strong positive correlations were observed between plant biomass and seed yield in all environments. Seed yield and pods plant^-1 were also highly associated in four of the five environments. Stepwise regression models indicated that the combination of pods plant^-1 and plant biomass consistently contributed to seed yield prediction, while other traits did not. Because both plant biomass and pods plant^-1 had moderate to high narrow sense heritability estimates and low GE interactions, they should be useful as indirect selection criteria to improve and stabilize seed yield in a breeding program. This revised version was published online in July 2006 with corrections to the Cover Date.     

Inheritance of pod cross-section in beans (Phaseolus vulgaris L.)

Summary OSU 5062, a green bean line with a tendency for pods to become creasebacked (wider than deep) in cross-section, was crossed to near-round-podded cultivars Oregon 83 and Slenderette, oval-podded Bountiful, and flat-podded Roma, to further clarify the inheritance of pod cross-section. Differences among generations in pod cross-section index (PCS), calculated as pod width/depth, were small in the near-round x creaseback crosses (1.07 and 1.01 for Oregon 83 and Slenderette versus 1.20 for OSU 5062). Bountiful (0.63) and Roma (0.50) differed to a greater extent from 5062. Generation means of individual plant PCS of the F₁ and F₂ were almost identical, were almost exactly intermediate between parents, and strongly supported additive inheritance. Backcross data generally supported additive inheritance, but in some cases indicated degrees of dominance of higher PCS. A generation means analysis indicated additive gene action, but deviations from an additive-dominance model were significant in each cross.     

Influence of Variable Amounts of Irrigation Water and Nitrogen Fertilizer on Growth, Yield and Water Use of Grain Sorghum

Sorghum hybrid CSH-6 was grown in fields in Delhi, India between July–November 1986 in order to study the effect of nitrogen nutrition and irrigation on dry matter accumulation, grain yield and water use. The treatments included 40 Kg Nha⁻¹ combined with two irrigations (30 DAS, 60 DAS), one irrigation (60 DAS) and no irrigation respectively. Rainfall during the crop season was only 17 cm. The unirrigated plants were considerably water stressed and exhibited very low leaf water potential, less leaf area, delayed anthesis, longer crop duration but shorter grain filling duration. The ears showed sterility and yield was only 0.41 t ha⁻¹ without nitrogen fertilization. Addition of nitrogen fertilizer had no significant effect on yield in unirrigated plants. A single irrigation 60 DAS increased yield due to increase in both grain number and grain weight per ear in fertilized and unfertilized crop respectively. Two irrigations in the unfertilized crop increased the yield to 2.2 t ha⁻¹ while similar treatment in the fertilized crop did not increase the yield significantly. Irrigation increased the WUE for grain yield. The results indicate that nitrogen stress and water stress reduced grain yield primarily through grain number rather than grain weight. Irrigation relieved both water stress and nutrient stress. Nitrogen nutrition was not beneficial under severe water stress conditions but was considerably helpful under mild stress. Biomass, grain yield and harvest index show significant correlation with preanthesis water use.     

Modification of Heterosis for Protein and Yield Components by Bean Common Mosaic Virus in Phaseolus vulgaris

The effect of bean common mosaic virus on yield, yield components, and seed protein content was studied in tour bean cultivars and their F1 hybrids. The results of this experiment showed 3 significant decrease for yield in diseased plants; the 100 seed weight was also significantly reduced in one of the diseased varieties. The percentage of protein was increased m virus-diseased plants: this increase was due to non-protein nitrogen. Usually, the percentage of heterosis in virus-diseased F1 hybrid of two susceptible varieties was decreased while in F1 hybrids of “susceptible × resistant” varieties it was increased. This study shows the danger of drawing conclusions in quantitative genetic studies then dealing with both diseased and healthy plants.     

Compensation-Induced Yield Gains in Mixtures of Common Bean (Phaseolus vulgaris L.)

Over the course of two planting seasons field trials were conducted at the CIAT farm in Palmira (Colombia). The experiments included nine tropical bean lines grown in pure stands and seven mixtures obtained by combining these lines on the basis of equal proportions in seed number.
In the case of several pure lines, inferior seed quality led to low germination and uneven population densities, especially in the first planting season. Analogously, missing plants also occurred with the corresponding mixtures. A significant and positive relationship between mixing induced yield gains and patchiness in mixtures was found, indicating that intergenotypic compensation was a major determinant for mixture effects on yield. In common bean as a tropical subsistence crop seed quality is known to be erratic. It is concluded that compensation effects in mixtures of common bean may be expected to be of practical importance regarding the improvement of yield stability over seasons.     

Water Stress and N Fertilization Effects on Yield and Smut Disease Incidence in Corn (Zea mays L.)

A 2-year field experiment was conducted in sandy clay loam soil to quantify the single and combined effects of N fertilization and water stress (WS) treatments on yield and smut incidence in corn. The plants were supplied with two different N rates (225 and 300 kg ha⁻¹) and subjected to 2 weeks' water stress, imposed at vegetative (Vg), tasselling (Ta), silking (Si) and grain-filling (Gf) growth stages. In addition, a control treatment (Cr) was also included to allow the plants to grow under unstressed conditions. The plants were artificially infected two times with Ustilago maydis spores and smut severity was recorded. At harvest, grain yield (GY), total biomass (Tbm) and harvest index (HI) were determined in smut-free (0) and smutted plants (S). Results have shown that GY₀ and GY_s were markedly reduced when the plants were subjected to WS. The depressing effects of WS were, comparatively, high at Gf. intermediate at Ta and Si. and low at Vg growth stages. Smut index (SI) data revealed that severity was higher on plants stressed at Gf than those exposed to WS at Vg, Ta or Si stages. Considerable variations were detected on Tbm and HI of smut-free and smutted plants, indicating the detrimental effect of plant water deficit at Gf stage. The combined effect of WS and N on Tbm_s showed mixed results, ranging from positive response for stressed plants at Si and Gf to little response at Cr, Vg or Ta stages at higher N rate. Although the effect of WS exposure on RYL data was dominant at Gf, the rate of yield depression was relatively higher at Ta and Si than the other growth stages, because of water deficit effects. The results proved that N fertilization did not conclusively alleviate the depressing effect of WS on grain yield losses.     

磷肥对芸豆几个品质性状的影响

本研究以‘NR’、‘品芸1号’、‘品芸2号’为试验材料,在不同磷肥施用量处理下,通过对三个品种蛋白质、脂肪、淀粉的测定、分析,探索磷肥对芸豆品质的调控效应。结果表明：在一定范围内,芸豆总淀粉含量随着磷肥用量的增加而增加;施用纯磷75 kg/hm2的对提高芸豆脂肪含量最有效;芸豆蛋白质含量在磷肥处理期间达到了显著差异,施用纯磷50 kg/hm2是提高蛋白质含量的最佳处理。