鸡传染性法氏囊病的防制

鸡传染性法氏囊病又称鸡传染性腔上囊病,是由传染性法氏囊病毒引起的一种急性、接触传染性疾病,是危害养禽业的主要疫病之一,以法氏囊发炎、坏死、萎缩和法氏囊内淋巴细胞严重受损为特征。该病发病率高,死亡率一般为5%～15%,能够     

鸡传染性法氏囊病的诊治

鸡传染性法氏囊病是由传染性法氏囊病毒引起的一种高接触性传染病,主要破坏中枢免疫系统法氏囊中的B淋巴细胞,导致免疫抑制。该病发病突然,病程短,发病率高。笔者在本学院动物医院坐诊期间,对几起典型病例的诊治做一总结,以供参考。1流行特点该病多发于3周龄至开产前的小鸡,3￣7周龄为本病高峰期,成年鸡对本病有一定抵抗力。3周龄以下的雏鸡感染后不表现明显的临床症状或轻度症状,但引起严重免疫抑制,易感染其他传染病。本病死亡率为10%￣20%,少数高达90%;常伴有并发症,以新城疫、马立克氏病、鸡大肠杆菌病为多见。2临床症状该病潜伏期为2…     

一例鸡传染性法氏囊病的诊治

鸡传染性法氏囊病(infectious bursal disease,IBD)是由传染性法氏囊病毒引起的3~12周龄鸡的高度接触性传染病,其临床主要表现为:法氏囊发炎、坏死、萎缩和法氏囊内淋巴细胞严重受损。IBDV属于双RNA病毒科禽双RNA病毒属,能破坏鸡的中枢免疫器官-法氏囊-造成机体的免疫抑制,导致感染鸡对其他致病因子的感染性增加和对其他疫苗的免疫应答能力的下降,给养鸡业带来了巨大的经济损失。1发病情况     

鸡传染性法氏囊病的防制措施

传染性法氏囊病是由传染性法氏囊病毒引起幼鸡的一种急性、高度接触性传染病，该病主要侵害法氏囊，法氏囊是禽类特有的淋巴器官，鸡孵出时法氏囊已存在，性成熟前发育最大，此后逐渐萎缩（鸡10个月），直至完全消失，本病具有发病率高、病程短、死亡率高的特点，同时还可导致免疫抑制，诱发多种疫病或使多种疫苗免疫接种失败。     

浅谈鸡传染性法氏囊病

鸡传染性法氏囊病（IBD）是由传染性法氏囊病毒引起的幼鸡的急性、高度接触性传染病。法氏囊病毒主要破坏鸡体的体液免疫中枢,影响淋巴细胞的成熟、分化以及使淋巴细胞大量流失,从而导致免疫能力下降甚至丧失,进而使正常疫苗免疫效果降低或无效。     

传染性法氏囊病病毒变异E株感染鸡细胞凋亡的研究

研究了传染性法氏囊病病毒（IBDV）变异E株人工感染28日龄SPF雏鸡后鸡法氏囊淋巴细胞的凋亡情况。电镜观察和DNA电泳分析结果表明,IBDV感染后12～48h,雏鸡法氏囊淋巴细胞出现典型细胞凋亡的形态学特征和生化特征;经流式细胞计检测和荧光染色观察,统计学分析表明,IBDV感染后24～48h,雏鸡法氏囊淋巴细胞凋亡数量显著增加（P<0.05或P<0.01）。试验结果揭示IBDV变异E株人工感染可以诱导雏鸡法氏囊淋巴细胞凋亡。     

鸡传染性法氏囊病的控制措施

近年来，鸡传染性法氏囊病（IBD）严重危害养鸡业发展，受到人们普遍地关注，该病又称鸡传染性腔上囊病，是由传染性法氏囊病毒引起的一种急性、接触传染性疾病，它以法氏囊发炎、坏死、萎缩和法氏囊内淋巴细胞严重受损为特征，从而引起鸡的免疫机能障碍，干扰各种疫苗的免疫效果，极易导致鸡只死亡，给养鸡业造成了极大的损失。     

鸡传染性法氏囊病及其免疫抑制研究进展

本文就鸡传染性法氏囊病的临床症状、病毒特性、免疫抑制效应及预防措施等进行综述,以加强对鸡传染性法氏囊病及所引起的免疫抑制效应的了解。     

浅谈鸡传染性法氏囊病的综合防治

鸡传染性法氏囊病是由病毒引起15日龄以内雏鸡和育成鸡的一种急性接触传染性疾病。该病主要是破坏鸡法氏囊淋巴细胞，使鸡的免疫机能发生障碍，体液免疫受剑抑制，本病除造成死亡外，主要增加了其它传染瘸易感性，易造成合并感染。该病发病率较高，特别是肉仔鸡，发病率最高，死亡率可高达30％。     

原位PCR检测鸡传染性法氏囊病病毒

采取临床病例鸡的法氏囊制成石蜡切片,经蛋白酶K处理、原位RT-PCR后,用地高辛精标记探针进行原位杂交检测传染性氏囊病病毒（IBDV）。结果10个病例有8个呈阳性,2个阴性。本试验尝试利用具有极高灵敏度的原位PCR方法,从组织中检测低拷贝甚至单拷贝的目标序列,以鉴别隐性感染或混合感染,为IBDV的诊断和分子流行病学的分析提供了一种新的手段。     

Morphologic changes in the bursa of fabricius of chickens after inoculation with infectious bursal disease virus.

Sequential morphologic changes in the bursa of Fabricius were studied after oral inoculation of 1-day-old chicks with infectious bursal disease virus (IBDV). The epithelial surface morphology was studied by scanning electron microscopy, whereas the IBDV replication was sequentially followed by immunofluorescence and transmission electron microscopy. The earliest detectable changes in the bursal epithelium were evident at postinoculation hour (PIH) 48. They were characterized by reduction in numbers and size of microvilli on the epithelial cells accompanied by gradual involution of the button-like bursal follicles. At PIH 96 some specimens showed localized surface erosions due to loss of epithelial cells. As the damage progressed, the infolding of the buttomlike follicles became more pronounced and the surface erosions became more extensive. Loss of surface epithelium exposed the underlying damaged bursal follicles which appeared to be bounded by columnar epithelium. Some follicles had lost almost all the lymphocytes and macrophages and appeared as empty craters. Intrafollicular replication of IBDV was detectable as early as PIH 24 by immunofluorescence technique. Viral replication primarily took place in the lymphoid follicles. Regeneration of the follicles was not seen up to postinoculation day 12, suggesting that the IBDV-induced bursal damage could be permanent.     

新城疫病毒人工感染鸡诱导胸腺和法氏囊细胞凋亡的初步研究

本研究用新城疫病毒(NDV)参考强毒株F48E8感染鸡,通过光镜和电镜观察鸡的胸腺和法氏囊淋巴绌胞凋亡的形态学特征,并进行统计学分析。NDV感染鸡后,其胸腺和法氏囊淋巴细胞凋亡数量显著增加(P＜0．01)。实验结果说明NDV参考强毒株F48E8人工感染鸡后可以诱导胸腺和法氏囊淋巴绌胞凋亡。     

鸡传染性法氏囊病病理组织学图谱

传染性法氏囊病(Infections Bursal Disease)又称传染性法氏囊炎,是由传染性法氏囊病病毒引起的一种破坏鸡免疫中枢器官--法氏囊的急性接触性传染病.自1957年起,先后发生于美国、法国、加拿大、日本等20多个国家和地区.     

Pathological changes of tracheal mucosa in chickens infected with infectious laryngotracheitis virus

Six-week-old chickens were inoculated via the posterior thoracic air sac with infectious laryngotracheitis virus. Chickens were sacrificed on various days through day 16 postinoculation (PI), and the trachea was examined by scanning electron microscopy (SEM) and light microscopy (LM). The pathological changes observed on day 1 PI were hypertrophy and hyperplasia of goblet cells. From day 3 PI, the epithelial cells protruded collectively and fused to form syncytia, which contained many intranuclear inclusion bodies. Subsequently, epithelial syncytia desquamated, one after another, and connective tissues were exposed in places. Serofibrinous exudate and detritus were abundant on the surface of the exposed connective tissues and seemed to form a pseudomembrane. On day 5 PI, the remaining epithelial cells began to repair the devastated mucosa just under the pseudomembrane. On day 6 PI, microvillus-rich regenerating epithelial cells were arranged like paving stones. On day 8 PI, the epithelial cells proliferated extensively and formed folds with cyst-like structures. By day 16 PI, the tracheal epithelium was covered with cilia and regained its normal histologic appearance.     

Sequential mitogen stimulation of peripheral blood lymphocytes from chickens inoculated with infectious bursal disease virus

Chickens were inoculated wih the pathogenic Edgar strain of infectious bursal disease virus at 1 week, 2 weeks, or 1 day of age. In the 3 experiments, phytohemagglutinin stimulation of peripheral blood lymphocytes was significantly decreased on day 3 or 4 after inoculation. Subsequently, on days 7 through 21, stimulations were similar between lymphocytes from inoculated birds and those from control birds. Pokeweed mitogen stimulation was affected minimally in virus-inoculated chickens. In each experiment, on day 7, the spontaneous [3H]thymidine uptake was greater in nonstimulated lymphocyte cultures from inoculated chickens than in such cultures from control chickens. In an additional experiment, chickens 1 week of age were exposed to a pathogenic vaccinal virus given in their water. The vaccinal virus exposure resulted in significant decrease of phytohemagglutinin stimulation of lymphocytes on days 3 and 7 of the experiment. A significant decrease in pokeweed mitogen stimulation was observed on day 10 after inoculation.     

"疫佳灵"对鸡法氏囊发育影响的组织学研究

免疫是机体识别自我物质和排除异己物质的一种保护性反应，它具有预防传染、维护自身稳定和免疫监视的机能。机体免疫机能的高低直接影响到机体的抗病力。资料证明，一些中草药的单方或复方具有增强免疫力的作用，而且中草药具有资源丰富、价格低廉、毒副作用小、无耐药性等优点，已引起国内兽医工作者的重视。本文对饮用中草药免疫增强剂——“疫佳灵”的雏鸡的法氏囊进行组织学的动态观察，旨在为免疫增强剂的开发和应用提供理论依据。