酵母抽提物替代血浆蛋白粉对断奶仔猪生产性能的影响

试验旨在研究酵母抽提物部分或全部替代血浆蛋白粉对断奶仔猪生产性能的影响。试验共选160头体重([6.20±0.10)kg]和日龄([26±2)d]相近的PIC断奶仔猪,分为4个处理,每个处理5个重复,每个重复8头仔猪。对照组(处理1)饲喂基础日粮,处理2饲喂含4%血浆蛋白粉(SDPP)日粮,处理3饲喂含2%SDPP+2%酵母抽提物的日粮,处理4饲喂含4%酵母抽提物日粮。试验期为28 d,所有仔猪自由采食和饮水。结果表明,与对照组相比,饲喂含4%酵母抽提物日粮能促进仔猪生长,试验全期仔猪日增重提高15%(P<0.05),饲料增重比降低7%(P<0.05);与含4%或2%SDPP日粮组相比,饲喂含4%酵母抽提物日粮并没有显著影响仔猪平均日采食量和日增重,但饲料增重比在试验前3周和试验全期均得到显著改善(降低7%～10%,P<0.05)。因此,酵母抽提物能促进断奶仔猪生长,部分或全部替代SDPP不影响仔猪增重,且能改善饲料转化率。     

Response of early-weaned pigs to an enterotoxigenic Escherichia coli (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody

Enterotoxigenic E. coli (ETEC) infection and resulting scours is a major problem for young pigs, especially when purified plant proteins are fed rather than spray-dried porcine plasma (SDPP). The effect of supplementing a pea protein isolate (PPI)-based diet with egg yolk antibodies (EYA) from laying hens immunized with ETEC K88 antigen on piglet performance, incidence of scours, and gut histology was studied in a 14-d trial. Ninety-six 10-d-old weaned pigs were assigned to five dietary treatments in a completely randomized design to give six replicate pens per treatment. The treatments were PPI without EYA (PPI-EYA), PPI with EYA (PPI+EYA), SDPP without EYA (SDPP-EYA), SDPP with EYA (SDPP+EYA), or a combination of PPI and SDPP (PPI+SDPP). Diets were formulated to similar nutrient levels and provided for ad libitum intake. Blood from all pigs was taken on d 0, 7, and 14 for determining plasma urea N (PUN). On d 7, pigs were orally challenged with 6 mL of 10(10) cfu/ mL ETEC K88. Piglets were weighed on d 7 and 14. On d 7, 8, and 14, four pigs per treatment were sacrificed to study the histology of the small intestine. Weekly feed intake, BW changes, and gain:feed were determined. Fecal swabs from 10 pigs per treatment were taken for a PCR test to detect K88 E. coli. Feed efficiency over the 14-d period was not affected (P > 0.78) by dietary treatment. Mean ADFI on an as-fed basis was lower (P < 0.002) in piglets fed PPI-EYA (64.3 g/d) compared with PPI+EYA (94.8 g/d) or SDPP (102 g/d) during wk 1. Piglets fed PPI-EYA tend to have a lower (P < 0.026) overall ADG (84 g/d) than those fed PPI+EYA (123 g/d) or SDPP (127 g/d) (P < 0.006)-based diets. Although scours was evident in all groups of pigs 6 h after the challenge, most of the piglets fed EYA- or SDPP-containing diets recovered 10 to 72 h postchallenge, whereas those fed PPI-EYA continued to have severe diarrhea, resulting in 33% mortality. The PCR results showed that a greater (P < 0.01) percentage of piglets fed PPI-EYA compared with those fed SDPP- or EYA-containing diets continued to shed ETEC K88 at the end of the 14-d study. Piglets fed PPI-EYA had shorter villi (P < 0.01), higher intestinal pH (P < 0.013), and higher PUN (P < 0.05) than those fed the SDPP- or EYA-containing diets during the entire 14-d study. It was concluded that specific EYA and SDPP could provide passive control of ETEC infection and potentially improve feed intake and weight gain in young pigs fed PPI.     

Growth performance and health status in weanling piglets fed spray-dried porcine plasma under typical Northern European conditions

The effect of inclusion of spray-dried porcine plasma (SDPP) in diets for weanling piglets was studied. The objectives were to determine whether SDPP would have positive effects on post-weaning piglet performance and health under typical Northern European conditions. In experiment 1, 160 weanling piglets were assigned randomly to a control diet or a diet containing 3% SDPP, which was added at the expense of both fishmeal and dried skim milk. In experiment 2, 264 weanling piglets were assigned to a control diet containing whey protein, a diet without whey protein but with SDPP or a diet containing both whey protein and SDPP. In essence, SDPP was added to the test diets at the expense of either whey protein or fishmeal. Piglets were fed the diets for 3 weeks. In experiment 1, the piglets fed the SDPP diet had a 7% higher average daily gain (ADG) and a 4% lower feed conversion ratio (FCR) (p < 0.05) during the first 3 weeks after weaning than did those fed the control diet. There were no differences in leucocyte counts or γ-globulin. In experiment 2 there were no significant differences in ADG and FCR among the dietary treatments. It is concluded that low amounts of SDPP in weanling diets can have positive effects on growth performance under Northern European conditions.     

苦玄参提取物对仔猪生长性能的影响研究

[目的]试验旨在探讨苦玄参提取物对仔猪生长性能的影响,为其在动物生产中更好地应用提供参考依据。[方法]选取体重相近的28~35日龄杜×长×大三元杂交的健康断奶仔猪250头,随机分为5组(A、B、C、D、E组),每组设5个重复,每个重复10头。其中,A、B、C组依次为苦玄参提取物高、中、低3个剂量试验组,分别在基础日粮中添加3.500、1.750和0.875g/kg苦玄参提取物;D组在基础日粮中添加对照药物七补散4.0g/kg;E组为空白对照组,饲喂基础日粮,预饲期7d,正试期30d。于试验正式开始的第0天、第15天和第30天7:00分别对各组的每一头试验猪进行空腹称重,并每天记录每个组试验猪的采食量,计算平均日采食量(ADFI)、平均日增重(ADG)和料重比(F/G)。[结果]添加苦玄参提取物后,A、B、C组仔猪的体重、ADG及ADFI,与E组相比显著或极显著(P<0.05或P<0.01)增加,而料重比降低;其中苦玄参提取物中剂量组仔猪的体重、平均日增重及平均日采食量提升最明显,料重比也极显著低于D组与E组(P<0.01)。[结论]苦玄参提取物对仔猪生长具有一定的促进作用,以1.750g/kg的添加剂量添加在饲料中效果最佳。     

饲粮添加银耳多糖对断奶仔猪生长性能和腹泻的影响

试验旨在研究银耳多糖对断奶仔猪生长性能和腹泻的影响。试验选取健康、体重接近的断奶仔猪72头,采用单因素完全随机化设计分为3个处理,即对照组(饲喂基础饲粮)、无抗组(在对照组饲粮基础上饲喂完全不含抗生素的饲粮)和TP组(在无抗组饲粮的基础上补充0.2%的银耳多糖)。每个处理3个重复,每个重复8头猪。试验期24 d。结果表明:与对照组相比,仔猪饲喂无抗饲粮日采食量增加0.95%、日增重增加1.75%、料重比下降了0.98%、腹泻率增加12.54%,但是对断奶仔猪的日采食量、日增重、料重比和腹泻率均无显著影响,表明不添加抗生素未对断奶仔猪生长性能造成负面影响;与无抗组相比,饲粮添加0.2%银耳多糖,仔猪日采食量提高9.76%、日增重提高12.98%、料重比下降2.46%、腹泻率下降17.80%,表明饲粮添加0.2%银耳多糖能够显著提高断奶仔猪的生长性能;与对照组相比,饲粮添加0.2%银耳多糖,仔猪日采食量提高10.80%、日增重提高14.95%、料重比下降3.41%、腹泻率下降7.49%,表明添加0.2%银耳多糖能够显著提高仔猪的生长性能。研究表明,饲粮中不添加抗生素会增加仔猪腹泻率,但未对其他生长性能造成负面影响;添加0.2%银耳多糖能够降低腹泻率、显著提高仔猪生长性能,并且能够替代抗生素发挥作用。     

Effects of spray-dried animal plasma on the growth performance of weaned piglets—A review

Spray-dried animal plasma sourced from bovine, porcine or other animal origin is often used as a main feed ingredient in the diets of weanling piglets to improve growth performance. The objective of this study was to determine the effect of animal plasma in diets on the performance of piglets in the post-weaning period, with and/or without pathogenic challenge, by undertaking a meta-analysis. Data were extracted from peer-reviewed reports published in scientific journals. The average initial weight of the piglets was 5.8 kg and the average initial age 19 days (2–56 days). The average duration of feeding animal plasma was 40 days. Average daily gain (ADG), feed intake (ADFI) and feed conversion ratio (FCR) were found to be 22–28 g/day, 20–27 g/day and −0.28 to 0.06 g/g. Generally, diet supplemented with spray-dried bovine plasma (SDBP) improved the ADG of the piglets and spray-dried porcine plasma (SDPP) led to increases in the ADFI. For the first week post-weaning alone, as the dietary animal plasma percentage increased there was an increase in ADG and ADFI; similarly, the latter two measures increased as weaning age increased. The evidence suggests that mainly IgG present in animal plasma prevents the binding of pathogens to the gut wall and reduces the incidence of sub-clinical infection in the post-weaning stage. Animal plasma containing IgG appears to be a useful in-feed supplement for piglets in the post-weaning phase.     

Spray-dried plasma in diets for weaned piglets: influence on growth and underlying mechanisms

Spray-dried animal plasma (SDAP) is a by-product of slaughter plants. The plasma obtained from slaughtered pigs or ruminants is spray-dried and used for the production of human foodstuffs and animal feeds. SDAP added to the diet of weaned piglets has considerable positive effects on the growth performance of piglets. In a meta-analysis, it was calculated from 68 comparisons between SDAP-containing diets and control diets that the SDAP-induced change in average daily gain (ADG) and average daily feed intake (ADFI) in the first 2 weeks after weaning was +26.8% and +24.5%, respectively. Two experiments demonstrated that dietary SDAP can reduce post-weaning diarrhoea. The aim of the research described in this thesis was to learn more about the mechanisms underlying the growth- and health-promoting properties of SDAP in the diet of weaned piglets. Results showed that dietary SDPP has positive effects on the post-weaning growth performance and health of piglets. These effects are more pronounced in piglets kept under suboptimal conditions and/or high infection pressure, and in piglets fed on diets lacking anti-microbial growth promoters. SDAP acts by influencing the gastrointestinal microflora: it appears to affect pathogenic bacteria rather than exert a general anti-bacterial effect leading to nutrient sparing, as has been described for anti-microbial growth promoters. SDAP has great potential as treatment for immuno-compromised mammals, such as neonates, and for animals in which antibiotic treatment is not possible, for instance when there is a ban against antibiotics or when multi-resistant bacteria are involved.     

Effect of salmon protein hydrolysate and spray-dried plasma protein on growth performance of weanling pigs

Two experiments, each consisting of 2 trials, were conducted to determine the effect of salmon protein hydrolysate (SPH) and spray-dried plasma protein (SDPP) fed during the first week postweaning and their subsequent effect on the growth performance of weanling pigs. Pigs were fed in a 3-phase feeding program with durations of 7 d for phase 1 in both Exp. 1 and 2; 14 or 15 d for phase 2 in Exp. 1 and 2, respectively; and 7 or 8 d for phase 3 in Exp. 1 and 2, respectively. Dietary treatments were fed only during phase 1, whereas the same diet was fed to all pigs in phases 2 and 3. Pigs were blocked by initial BW and sex, and littermates were balanced across treatments. Data from the 2 trials within each experiment were combined and analyzed together; no treatment × trial interactions (P > 0.10) were observed. In Exp. 1, a total of 324 weanling pigs (10 replications of 5 or 6 pigs per pen) with an average initial BW of 6.4 ± 1.3 kg were assigned to 1) a control diet with no SPH or SDPP, 2) 1.5% SPH, 3) 3.0% SPH, 4) 1.5% SDPP, 5) 3.0% SDPP, or 6) 1.5% SPH + 1.5% SDPP. Experiment 2 was similar to Exp. 1, but red blood cells were removed from all diets to reduce diet complexity. In Exp. 2, weanling pigs (n = 320, 14 replications of 5 or 6 pigs per pen) with an average initial BW of 5.4 ± 1.2 kg were assigned to 1) a control diet with no SPH or SDPP, 2) 1.5% SPH, 3) 1.5% SDPP, or 4) 1.5% SPH + 1.5% SDPP. Three batches of SPH were used, and each batch was analyzed for AA composition. In Exp. 1, the inclusion of SDPP or SPH during phase 1 did not affect (P > 0.10) ADG, ADFI, or G:F compared with those of pigs fed the control diet. No carryover effects on growth performance were observed in any of the subsequent phases. Overall, G:F was greater (P = 0.08) in pigs fed the 1.5% diets compared with those fed the 3.0% diets. In Exp. 2, no differences (P > 0.10) were observed in ADG, ADFI, or G:F among pigs fed the SPH or SDPP diets compared with those of pigs fed the control diet. Pigs fed the combined diet had greater (P < 0.10) overall ADFI compared with that of pigs fed the control diet, but ADFI was similar to that of pigs fed the SPH and SDPP diets. These results indicate that inclusion of up to 3% SDPP or SPH in diets fed during the first week postweaning did not affect the growth performance of weanling pigs, and no subsequent carryover effects were observed. Salmon protein hydrolysate did not affect the growth performance of weanling pigs and may be considered an alternative protein source in diets for weanling pigs.     

Belly-nosing in early-weaned piglets is not influenced by diet quality or the presence of milk in the diet

Early weaning of piglets can lead to an increase in belly-nosing and other oral-nasal behavior (nosing, chewing, or sucking other piglets), but the causative factors involved in these behavior patterns are largely unknown. Because these behavior patterns resemble massaging the udder and sucking, they may be associated with feeding. The objectives of this study were to determine any effect of diet quality or the presence of milk in the diet on belly-nosing behavior of piglets weaned at 14 to 18 d. During the first 2 wk after weaning, piglets were fed diets differing in quality and inclusion of milk products. Six replicates of eight piglets per replicate, blocked by initial body weight, (n = 192) were offered one of four dietary treatments: HQM: high quality, high in milk products; HQ: high quality, no milk products; PQ: poor quality, no milk products; HQ+MR: high quality, no milk products (as HQ) sprayed with milk replacer five times daily. Thereafter, the piglets were fed a standard nursery diet. Feed intake was measured daily for wk 1 and again at the end of wk 2. Behavior was recorded every 5 min during two 4-h periods on d 2 to 7, 10, 14, 17 and 21 after weaning. Dietary treatment influenced ADFI and ADG during wk 1. Average daily feed intake (P < 0.05) and ADG (P < 0.05) of piglets on PQ were less than those of piglets on the other treatments. During wk 2, ADFI (P > 0.10) and ADG (P > 0.10) were the same across all treatments. Overall, ADFI was not influenced by the inclusion of milk products in the diet or the addition of milk replacer (P > 0.10); however, ADG was. Piglets on HQM had higher ADG than those on HQ during wk 2 (P < 0.05) and 3 after weaning (P < 0.05). However, milk replacer did not influence ADG (P > 0.10). Although the dietary treatments did affect ADFI and ADG, there were no effects on any behavior pattern recorded, including time spent at the feeder (P > 0.10). Lower weight-for-age piglets performed more oral-nasal behavior, in total, than higher weight-for-age piglets (P < 0.03). Neither feeding a poor-quality diet nor the presence of milk in the diet had an effect on belly-nosing or other oral-nasal behavior patterns during the first 3 wk after weaning. Belly-nosing does not seem to be associated with feeding.     

蛋白酶解物对早期断奶仔猪肠黏膜形态和生长性能的影响

本文旨在研究自制蛋白酶解物对早期断奶仔猪生长性能的影响。选用40头健康杜×长×大三元杂交商品仔猪(约3.5 kg/头),随机分为5个处理组(1个对照组,4个试验组),每个处理组设4个重复,每重复2头。对照组添加1.5%血浆蛋白粉,试验1~4组分别添加2%玉米蛋白粉酶解物(HCPP)、豆粕酶解物(HSM)、鱼粉酶解物(HFM)和酵母酶解物(HY)。仔猪14日龄断奶,预试期2 d,正试期14 d。试验测定了早期断奶仔猪生长性能和肠黏膜形态。结果表明:日粮中添加2%酵母酶解物通过保护小肠黏膜的正常形态结构,能有效地提高早期断奶仔猪平均日增重(24.64%,P<0.05)和日采食量(7.80%),并改善饲料转化率(13.68%)。     

高铜对断奶仔猪生长性能及血清激素水平的影响

选用60头28日龄±2日龄“长大定”三元杂交断奶仔猪,按体重和性别随机分成3组,每组20头。Ⅰ组(对照组)仔猪饲喂基础日粮;Ⅱ组仔猪在断奶后14d内饲喂基础日粮 250mg/kg铜(CuSO4.5H2O),然后再饲喂基础日粮;Ⅲ组仔猪饲喂基础日粮 250mg/kg铜(CuSO4.5H2O)。试验期为28d。所有实验猪均于断奶后0,7,14d和28d称重,计算采食量,观察断奶后腹泻情况。同时每组固定10头仔猪,于断奶后0,7,14d和28d,经前腔静脉采血,测定部分血清激素水平。结果表明,高铜日粮能显著提高断奶仔猪体重(AW)、平均日增重(ADG)和日采食量(ADFI),降低料重比(F/G)和断奶后腹泻率,显著提高断奶仔猪血清GHI、NS、T3和T4的水平。     

Dietary specific antibodies in spray-dried immune plasma prevent enterotoxigenic Escherichia coli F4 (ETEC) post weaning diarrhoea in piglets

In order to establish the mechanism of spray dried plasma powder (SDPP) in improving pig health and performance, a diet containing either 8% SDPP, spray dried immune plasma powder (SDIPP), or control protein (soybean and whey) ration was fed to piglets in an experimental model of enterotoxigenic Escherichia coli F4 (ETEC) post-weaning diarrhoea (PWD). SDIPP was obtained from pigs immunized with a vaccine containing ETEC fimbrial subunit F4 and heat-labile toxin (LT), and SDPP from non-immunized controls. Average daily growth (ADG) was determined, and daily samples of rectal faeces were assessed for diarrhoea (as percentage of dry matter), and ETEC excretion (in CFU/g). SDPP and SDIPP significantly (p<0.05) reduced diarrhoea, and SDIPP significantly reduced ETEC excretion. ADG was not significantly (p>0.05) affected. After the experiment, 30% of piglets tested F4 receptor positive (F4R+). A significant correlation between F4R status and morbidity was found. In F4R+ animals, SDIPP significantly improved diarrhoea and ADG, and decreased ETEC excretion, and SDPP significantly improved diarrhoea and ADG. Surprisingly, SDPP reduced diarrhoea in F4R+ animals without significant reduction of ETEC excretion, which is most likely related to the presence of anti-LT antibodies in SDPP. The results show that oral protection against ETEC by SDPP is attributable to spontaneous antibodies, in this case anti-LT antibodies. Furthermore, the results indicate that the combination of anti-LT and anti-F4 antibodies as in SDIPP is most effective in ETEC prevention. Finally, the F4R distribution in the herd should be taken into account to correctly assess efficacy.     

大豆磷脂油有效能值评定及其在仔猪饲粮中的应用效果

本研究包括2个试验,试验1旨在评定大豆磷脂油的有效能值;试验2旨在研究大豆磷脂油对断奶仔猪生长性能、血清脂质代谢及直肠微生物数量的影响。试验1(代谢试验):选用16头平均体重(27.54±1.20)kg的"杜×长×大"去势公猪,随机分为2个组,每个组8个重复,每个重复1头猪,分别饲喂基础饲粮和4%大豆磷脂油替换基础饲粮的试验饲粮,预试期5d,正试期4 d。试验2(饲养试验):选用54头23日龄断奶、初始体重(6.50±0.48)kg"杜×长×大"仔猪,按体重相近原则随机分为3个组,每个组6个重复,每个重复3头猪,分别饲喂基础饲粮(含2%大豆油,对照组)、1.0%磷脂油饲粮(大豆磷脂油等量替代基础饲粮中50%大豆油,即含1.0%大豆油+1.0%磷脂油)和1.5%磷脂油饲粮(大豆磷脂油等能替代基础饲粮中50%大豆油,即含1.0%大豆油+1.5%磷脂油),试验期35 d。代谢试验结果表明:大豆磷脂油的表观消化能为31.32 MJ/kg,表观代谢能为30.07 MJ/kg。饲养试验结果表明,与对照组相比:1)第15~35天,1.0%、1.5%磷脂油饲粮组仔猪平均日采食量(ADFI)分别提高了6.23%和3.13%(P0.05),平均日增重(ADG)分别提高了6.66%和5.28%(P0.05);第1~35天,1.0%磷脂油饲粮组仔猪ADFI和ADG分别提高了5.34%和5.64%(P0.05)。2)1.0%磷脂油饲粮组仔猪腹泻率和腹泻指数分别降低了5.81%和13.41%(P0.05)。3)1.0%磷脂油饲粮组仔猪血清甘油三酯和游离脂肪酸的含量分别降低了10.91%和12.80%(P0.05);1.5%磷脂油饲粮组血清甘油三酯、总胆固醇和高密度脂蛋白胆固醇含量分别降低了7.27%、5.33%和10.53%(P0.05)。4)第15天,1.0%和1.5%磷脂油饲粮组仔猪直肠粪便大肠杆菌数量分别降低了4.88%和4.12%(P0.05);第36天,1.0%磷脂油饲粮组仔猪直肠粪便大肠杆菌数量降低了4.88%(P0.05)。综上可见,大豆磷脂油的表观消化能为31.32 MJ/kg,表观代谢能为30.07 MJ/kg;饲粮添加1.0%大豆磷脂油等量替代基础饲粮中的大豆油,可一定程度提高仔猪ADFI和ADG,降低腹泻率和直肠粪便大肠杆菌数量。     

Nutritional evaluation of egg byproducts in diets for early-weaned pigs

A total of 272 Cotswold pigs (17 +/- 1 d) were utilized in three experiments to evaluate the nutritive value of spray-dried egg proteins for early-weaned pigs. In all experiments, pigs were stratified by sex and initial BW and then assigned randomly to experimental diets. In Exp. 1, four corn-soybean meal-based diets containing 7% of either spray-dried porcine plasma (SDPP), spray-dried technical albumen (SDTA), SDTA stored at 70 degrees C for 3 d (SDTA-ht), or spray-dried whole egg (SDWE) were assigned to five pens each with four pigs for a 3-wk study period. Average daily gain, ADFI, and gain:feed ratio (G:F) were determined. At the end of wk 3, five pigs per treatment were killed to determine ileal AA and energy digestibilities, as well as Enterobacteriaceae counts. Compared with the SDPP diet, ADG and G:F were lower (P < 0.05) for SDTA-, SDTA-ht- and SDWE-containing diets. Apparent ileal digestibilities of cystine, histidine, isoleucine, methionine, and threonine in the SDPP diet were lower (P < 0.05) than in diets containing spray-dried egg products. Ileal digestible energy content did not differ (P > 0.05) in all diets (3.1 to 3.2 Mcal/kg). Enterobacteriaceae counts were lower in the SDTA-ht diet than in either the SDTA or SDWE diets (P < 0.05). In Exp. 2, the effect of substituting SDPP with varying levels of SDTA was investigated. Diets were randomly assigned to five pens (except for the 100% SDTA diet, which had four pens), each with four pigs. Average daily gain, ADFI, and G:F decreased linearly as the level of SDTA was increased in the diet (P < 0.05). Replacing SDPP with SDTA at 25 or 50% had no effect on pig performance (P > 0.10). In Exp. 3, phase I diets containing 0, 25, or 50% SDTA in place of SDPP (7% of the diet) were each assigned at random to eight pens each with four pigs for a 14-d period, after which all pigs were switched to a common phase II diet lacking both SDPP and SDTA for another 14 d. Average daily feed intake and ADG did not differ among all diets in phase I and II and overall (d 0 to 28). Pigs fed the diet containing 50% SDTA in phase I had lower (P < 0.05) G:F than those fed the SDPP diet. The results indicate that technical albumen can replace 25 to 50% of SDPP in early-weaned pig diets without compromising performance, and further suggest that heat-treated SDTA may affect intestinal microbial population in pigs.     

Response of early-weaned pigs to an enterotoxigenic Escherichia coli (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody,zinc oxide,fumaric acid,or antibiotic

The effect of feeding diets containing either spray-dried porcine plasma (SDPP) or pea protein-isolate (PPI) supplemented with either egg yolk antibodies (EYA) from hens immunized with enterotoxigenic Escherichia coli (ETEC) (K88 and F18) antigens, ZnO, fumaric acid (FA), or carbadox (AB) on pig performance, incidence of scours, and gut morphology was studied in a 14-d experiment. Ninety 10-d-old weaned pigs were assigned to six dietary treatments in a completely randomized design to give five pens per treatment with three pigs per pen. The diets were SDPP without EYA (SDPP - EYA), PPI without EYA (PPI - EYA), PPI with EYA (PPI + EYA), PPI with ZnO (PPI + ZnO), PPI with FA (PPI + FA), or PPI with AB (PPI + AB). Diets were formulated to similar nutrient levels, with AB, EYA, FA, and ZnO at 0.25, 0.5, 2.0, and 0.4% of the diet, respectively. Pigs were weighed and bled on d 0, 7, and 14 to determine plasma urea N (PUN). Pigs were orally challenged with a 6-mL dose of 10(10) cfu/mL ETEC (K88) on d 7. On d 14, three pigs per treatment were killed to obtain sections of the small intestine for histological measurements. Weekly feed intake, BW changes, and gain:feed were determined. Incidence of scours and scour scores were monitored and fecal swabs were taken before and after ETEC challenge for PCR test to detect ETEC (K88). Feeding SDPP or supplementing PPI-based diets with EYA, ZnO, FA, or AB did not affect (P > 0.05) ADG, ADFI (as-fed basis), or gain:feed throughout the study. However, pigs fed PPI - EYA tended to have lower (P = 0.08) ADFI during wk 2 (137.9 g/d) and lower (P < 0.10) ADG from d 0 to 14 (100.1 g/d) than those fed the SDPP - EYA (156.6 g/d), PPI + EYA (151.2 g/d), PPI + ZnO (158.9 g/ d), PPI + FA (155.4 g/d), and PPI + AB (152.6 g/d) diets. Although scours was evident in all pigs 8 h after the ETEC challenge, it lasted only 3 to 5 d in pigs fed SDPP or PPI supplemented with EYA, ZnO, FA, or AB. Pigs fed PPI - EYA continued to have severe diarrhea, resulting in 40% mortality vs. 13% or less in the other groups. The PCR results showed that 81% of PPI-fed pigs continued to shed ETEC K88 7 d after ETEC challenge. Pigs fed PPI-EYA had shorter villi (P < 0.05), reduced villi:crypt ratio (P < 0.003), and higher intestinal pH (P < 0.001) and PUN (P < 0.001) than those fed SDPP or PPI supplemented with EYA, ZnO, FA, and AB. In conclusion, SDPP, EYA, ZnO, FA, and AB may have provided passive control to ETEC (K88) infection and potentially enabled young pigs to efficiently utilize a PPI-based diet.     

饲料的不同形态与状态对早期断奶仔猪生产性能的影响

试验采用2×2因子设计,共分4个处理,即粉料和颗粒料2种类型,每种类型2种状态(液态和固态)。试验选用96头(21±1)d断奶的三元(D×L×Y)杂交仔猪,试验期21 d。其中液态料按12∶.5的料水比进行浸泡。试验期间考察仔猪的耗料量、增重、腹泻情况、粪便pH值及木糖吸收情况等,并测定饲料的pH值和淀粉糊化度。结果表明:①与粉料组相比,颗粒料组仔猪平均日增重提高了9.7%(P<0.05),平均日采食量提高了13.5%(P<0.10)。②颗粒料的淀粉糊化度比粉料高15%-16%。③液态组仔猪全期平均日采食量和日增重均高于固态组(P<0.05),分别提高了10.3%和12.9%。④液态料组仔猪血清木糖含量比固态料组高52%(P<0.05),且腹泻程度显著(P<0.05)降低。     

壳聚糖对仔猪生长性能及血清中类胰岛素生长因子-Ⅰ和生长激素水平的影响

本文旨在研究日粮中添加不同剂量壳聚糖对仔猪生产性能及血清中类胰岛素生长因子-I(IGF-I)和生长激素(GH)水平的影响。试验选择60日龄大白×长白×VI系杂交仔猪96头,随机分为4个日粮处理组,每个处理3个重复,每个重复8头,进行为期21 d的饲养试验。4种日粮分别是在基础日粮中添加0、0 .01 %、0 .05 %、0.1 %的壳聚糖配制而成。结果表明,壳聚糖对仔猪生长性能的影响呈二次剂量依赖关系,添加0 .01 %~0 .05 %壳聚糖能够提高仔猪平均日增重、平均日采食量及增重耗料比,并可提高仔猪血清中IGF-Ι和GH含量。而添加更高水平时生长性能却有下降趋势。因此,在本试验条件下初步得出,0 .01 %~0 .05 %为壳聚糖在仔猪日粮中的适宜添加量。     

Raw potato starch in weaned pig diets and its influence on postweaning scours and the molecular microbial ecology of the digestive tract

We evaluated the effect of raw potato starch (RPS) on growth performance, postweaning diarrhea, and gastrointestinal microbial populations in weaned piglets. Eighty-four piglets were weaned at 17 +/- 2 d of age with an average BW of 6.0 +/- 0.9 kg. Pigs were blocked by BW and assigned to 1 of 4 diets in a randomized complete block design with 7 replicate pens per diet and 3 pigs per pen. Treatments were 1) a positive control (PC) containing an antibiotic, 2) a negative control (NC) with no RPS and no antibiotic, 3) NC + 7% RPS (7% RPS), and 4) NC + 14% RPS (14% RPS). Diets were corn-wheat-soybean meal-based and formulated to meet NRC (1998) recommendations. The ADG, ADFI, and G:F ratio were determined weekly. Fecal consistency (FC) scoring was determined daily. After wk 3, 1 pig with a BW closest to the pen mean was killed to evaluate ileal and colonic mucosal-attached Escherichia coli and lactic acid bacteria, as well as digesta pH, VFA, and ammonia N concentrations. The DNA was extracted from ileum and colon digesta and used for molecular microbial evaluations using terminal-RFLP analysis of 16S rDNA genes. The ADG for wk 1 was greater (P < 0.01) for the PC diet, but diet had no effect on ADG during wk 3. The ADFI did not differ among treatments during the first 2 wk, and ADFI was least for 7% RPS diet during wk 3. The NC diet had a greater (P < 0.05) FC score during wk 1 than other treatments, but diet had no effect on FC score during wk 2 and 3. Diets had no effect on the colon lactic acid bacterial counts; however, the PC diet had decreased (P < 0.05) colon E. coli counts than other treatments. Ileum and colon digesta pH and total VFA concentrations did not differ among treatments. Pigs fed with 7 and 14% RPS diets had greater (P < 0.05) ileum ammonia N concentration compared with pigs fed with other diets. There was more diarrhea (P < 0.05) in the 14% than the 7% RPS and control treatments at d 21. This difference correlated with a decline (P < 0.05) in microbial diversity in the colon. We concluded that 7% RPS can be used to prevent postweaning diarrhea in weaned piglets, but there are no effects on growth performance.     

Improvement of growth performance and sanitary status of weaned piglets fed a bovine colostrum-supplemented diet

The present study investigated the effect of 3 different durations of feeding a diet supplemented with defatted bovine colostrum (Col) on growth performance and sanitary status of the weaned piglet. At 28 d of age, piglets were weaned and fed 1 of the 2 following diets: a control (Ctrl) starter diet or a starter diet supplemented with Col. Two experiments were conducted. In Exp. 1, 310 piglets (12 pens consisting of 10 piglets/pen and 10 pens consisting of 19 piglets/pen) were allocated to 1 of the 2 dietary treatments for 12 d. In Exp. 2, 522 piglets (18 pens consisting of 10 piglets/pen and 18 pens consisting of 19 piglets/pen) were allocated to 1 of the following 3 dietary treatments: fed the Ctrl diet from d 1 to 12 (Ctrl), Col diet from d 1 to 4 and then the Ctrl diet up to d 12 (Col-4d), or the Col diet from d 1 to 6 and then the Ctrl diet up to d 12 (Col-6d). For both experiments, a commercial second-phase diet was fed to piglets from d 12 to 46. Feed intake, growth performance, and cleanliness of floor and hindquarters of animals were investigated during the first 7 wk postweaning. In Exp. 1, from d 0 to 12, ADFI, ADG, and G:F were 16 (P = 0.004), 23 (P < 0.001), and 5% (P = 0.069) greater, respectively, in Col piglets compared with Ctrl piglets. Thereafter, ADFI and ADG were 7 (P < 0.001) and 9% (P < 0.001) greater, respectively, in Col piglets than Ctrl piglets (d 12 to 46). On d 12 after weaning, piglets fed the Col diet had more normal feces (+13%) and less soft or liquid feces (-9 and -4%, respectively) than piglets fed the Ctrl diet (P = 0.06). Compared with Ctrl piglets, feeding the Col diet led to more days with normal feces for the floor cleanliness (+22%; P < 0.001) from d 7 to 11. In Exp. 2, compared with Ctrl piglets, ADFI, ADG, and G:F were 8, 23, and 13% greater (P < 0.05) in Col-6d piglets from d 0 to 9, whereas values for Col-4d piglets were intermediate and did not differ from the values of the other dietary treatments. On d 9 after weaning, piglets fed the Col-4d or the Col-6d diet had more normal feces (+6 and +4%, respectively) and less liquid feces (-4 and -3%, respectively) than piglets fed the Ctrl diet (P = 0.08). No long lasting effects were observed thereafter. In conclusion, there was a reduction of weaning-induced growth check and diarrheal episodes in weaned piglets fed the Col diet. The beneficial effects of the bovine colostrum were observed beyond the period of treatment when the supplementation covered the first 6 d postweaning, which corresponded to the acute phase of postweaning digestive disturbances.     

Effects of feeding grains naturally contaminated with Fusarium mycotoxins with and without a polymeric glucomannan mycotoxin adsorbent on reproductive performance and serum chemistry of pregnant gilts

Contamination of animal feedstuffs with Fusarium mycotoxins can cause reduced feed intake and hyperaminoacidemia resulting from reduced hepatic protein synthesis. The current study investigated the effects of feeding grains naturally contaminated with Fusarium mycotoxins on reproductive performance, serum chemistry, ADFI, and ADG of gilts, and tested the ability of a polymeric glucomannan mycotoxin adsorbent (GMA) to reduce or eliminate the effects of the contaminated feeds. Thirty-six Yorkshire gilts were fed 3 diets (n = 12 gilts/diet) from 91 +/- 3 d of gestation until farrowing. Diets included 1) control, 2) contaminated grains, and 3) contaminated grains + 0.2% GMA. Diets contaminated with Fusarium mycotoxins did not affect ADFI (P = 0.24), but ADG (P = 0.029) and G:F (P = 0.047) were reduced. Serum concentrations of beta-hydroxybutyrate, haptoglobin, protein, albumin, globulin, urea, glucose, cholesterol, Ca, Na, Mg, P, K, and Cl, and hepatic enzyme activities were not affected by diet. The frequency of stillborn piglets was greater (P = 0.03) for gilts fed contaminated grains compared with that of gilts fed contaminated grains + GMA. The feeding of contaminated grains + GMA also increased (P = 0.026) the percentage of pigs born alive compared with gilts fed the contaminated diets. In conclusion, feeding gilts diets that are naturally contaminated with Fusarium mycotoxins can increase the incidence of stillborn piglets and this effect can be reduced by dietary supplementation with GMA.