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对松材线虫7个株系和拟松材线虫2个株系的线粒体DNA随机扩增多态性分析结果显示,松材线虫和拟松材线虫种间和种内都表现出很大的变异性,但种间变异明显大于种内变异,因而支持将松材线虫和拟松材线虫划分为两个不同的种.两个拟松材线虫株系(法国株系和日本株系)之间的相似值和松材线虫各株系间的相似值极为接近,由此看来,这两个株系的变异为种内变异,应同属于一个种.聚类分析的结果表明,松材线虫和拟松材线虫系两个明显不同的类群,即松材线虫类群和拟松材线虫类群.松材线虫类群又分为两个亚组,一个是以日本株系为代表,另一个是以北美洲(美国和加拿大)株系为代表. 相似文献
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遗传背景相对一致、致病力稳定的植物线虫材料是开展其遗传学和致病机制研究的基础。对分别来自中国和日本的4个松材线虫(Bursaphelenchus xylophilus)和1个拟松材线虫(B.mucronatus)虫株采用两条幼虫配对在葡萄孢(Botrytis cinerea)基质上进行单异活体共培养的方法获得了若干个遗传背景相对一致线虫家系,家系成功率为13.3%~20%。线虫家系在盛有灰葡萄孢培养基质的、直径为9 cm的培养皿上取食完全所需的时间为10~44天。对不同家系进行了致病力测定表明,家系间的致病力分化明显。综合考虑感病率、感病指数、接种松苗死亡历期等指标,其中松材线虫家系AN19#F1的致病力最强,而拟松材线虫虫株AN5#所建家系都没有表现致病力。 相似文献
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Bursaphelenchus xylophilus is the pathogen that causes pine wilt disease, which has greatly damaged forests and ecosystems in countries of East Asia and Europe.Bursaphelenchus mucronatus is closely related to B. xylophilus in morphology and host plant specificity. A longrunning debate has existed regarding whether these two species can successfully produce hybrid offspring. In the present study, we performed in the laboratory, hybridization of two B. xylophilus nematode isolates from China and Japan and three B. mucronatus isolates from China, Japan and France. Nematode isolates of B. xylophilus were successfully crossed with B. mucronatus isolates, and the rate of hybridization was relatively high; however, some hybrid offspring died. Successful hybridization occurred between B. xylophilus and B. mucronatus isolates from China, and 22 generations of hybrids were produced. All F1 hybrids could be backcrossed with their parents and produce offspring. Variation in mucro length among the hybrid offspring and their parents was observed. The hybrid offspring and their parents were inoculated into 3-months-old black pine(Pinus tunbergii) seedlings. Weaker pathogenicity of hybrid offspring was observed compared with that of their parents, and significantly fewer offspring nematodes than parents were reisolated from pines. Therefore, the offspring of B. xylophilus and B. mucronatus may exist in the forest and could influence disease epidemics. 相似文献
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采用多指标综合评价方法对贵州省拟松材线虫病的危险性进行了分析。通过风险性分析和计算,得出拟松材线虫是松树的重要病害种类,其R值为1.74,属于中度危险的林业有害生物。在此基础上,探讨了贵州省拟松材线虫病的风险管理方法。 相似文献
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A polymerase chain reaction-restriction fragment length polymorphism analysis was used to discriminate isolates of Bursaphelenchus xylophilus and B. mucronatus. The amplifications of B. xylophilus isolates yielded one fragment of approximately 890 bp and that of B. mucronatus was about 930 bp. Digestion of amplified products of each nematode isolate with five restriction endonucleases revealed the
following results: 1) Dra I digestion of the internal transcribed spacer (ITS) products of B. xylophilus populations yielded two fragments of 510 and 380 bp. Dra I could not digest the ITS products of B. mucronatus populations; 2) Sal I could not digest the ITS products of all B. xylophilus populations, but it could digest those of B. mucronatus populations into two fragments, which were 720 and 220 bp; 3) digested products of four B. xylophilus populations by Msp I yielded two fragments of 530 and 360 bp, except GZ02, which could not be digested. B. mucronatus populations yielded three fragments: 340, 290, and 180 bp; 4) all populations of B. xylophilus and B. mucronatus could not be digested by Apa I; 5) digestion of the ITS products of B. xylophilus and B. mucronatus yielded two fragments of 520 and 370 bp, and 530 and 400 bp respectively. The restriction endonucleases Dra I and Sal I could be used to identify B. xylophilus and B. mucronatus. Because the results of digestion of B. xylophilus and B. mucronatus were markedly different, they were very easy to be identified and applied; Msp I and Xho I were not suitable for identification of B. xylophilus and B. mucronatus and Apa I could not identify and distinguish between B. xylophilus and B. mucronatus.
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Translated from Journal of Nanjing Forestry University, 2005, 30(4): 5–9 [译自: 南京林业大学学报] 相似文献
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采用SDS法提取线虫基因组DNA,分别选取了6种随机引物对部分地区的10株松材线虫和10株拟松材线虫进行随机扩增和亲缘关系比较。RAPD的结果可以明显区分松材线虫和拟松材线虫,与形态学分类相吻合。聚类分析的结果也表明,线虫种内和种间均存在差异,但种间差异明显大于种内差异,同种线虫在同一地区或临近地区的同源性明显高于其它地区,同一地区内寄主对线虫种类分布的影响较小。该方法具有所需样品量少、检测灵敏度高、结果准确等优点,为松材线虫种群的亲缘关系、传播途径及其检测防疫等方面提供一种灵敏、可靠的研究方法。 相似文献