不同检测方法对猪瘟病毒抗体检测比较研究

为了找出适用于不同级别畜牧兽医站检测猪瘟病毒抗体的方法，本文采用猪瘟病毒抗体ELISA检测法和猪瘟病毒抗体快速检测卡进行猪瘟病毒抗体检测，通过统计分析，结果表明，猪瘟病毒抗体ELISA检测法适用于县级兽医实验室检测，结果可靠、准确；猪瘟病毒抗体快速检测卡适用于乡镇畜牧兽医站或养殖场自行检测，方法简单，快速，易操作，但准确性较差，为不同级别和地方的猪瘟病毒抗体检测方法的选择提供重要参考。     

猪瘟疫苗的种类与使用方法

猪瘟是一种传染性非常强的传染病，常给养猪业造成毁灭性损失。预防猪瘟最有效的方法就是接种猪瘟疫苗。为了使广大养猪户正确选择和有效使用猪瘟疫苗，这里将猪瘟疫苗的种类和使用方法简介如下。     

用ELISA猪瘟抗原检测试剂盒诊断猪瘟

近年来，猪瘟的流行特征发生了重要变化，出现了以慢性、阴性感染为主的流行趋势，致使猪瘟的临床诊断更为困难。目前实验室检测猪瘟的方法很多，以抗原一抗体反应为基础的酶联免疫吸附试验(ELISA)被广泛采用，ELISA是快速检测猪瘟的有效方法。笔者利用ELISA猪瘟抗原检测试剂盒，对某猪场送检病料进行猪瘟诊断，取得了较好的效果。     

种猪场猪瘟的控制与净化

猪瘟是猪瘟病毒（CSFV）引起的一种猪的急性、烈性、接触性传染病，在我国被列为一类传染病，国际动物卫生组织将列为其A类动物传染病。长期以来，猪瘟多以非典型性、慢性及隐性形式出现，给养猪业造成巨大的经济损失。在规模化猪场猪瘟控制与净化过程中，我们采取接种免疫疫苗为主的方法，并配有特异、敏感、便于操作的检测、监测手段，应用猪瘟病毒RT-PCR方法进行初筛，如有阳性，再进行猪瘟病毒野毒株荧光RT-PCR方法鉴别检测，若出现阳性，则进行扑杀。这一套猪瘟净化技术措施的实施在猪瘟净化过程中取得了较好的净化效果。     

猪瘟免疫失败的原因和防制对策

猪瘟（HogCholera．HC）是猪的一种高度接触传染性病毒病。根据临床过程分为急性、慢性、迟发型三种。急性猪瘟是由强毒力猪瘟病毒引起，发病率和死亡率都很高。慢性猪瘟和亚临床型猪瘟是低毒力猪瘟病毒株感染引起，统称为非典型猪瘟，临床症状和病理变化与急性猪瘟相比，表现为多样性，而且特征很不明显，病程一般较长，易与其他疾病混淆，依靠常规方法很难确诊，     

鸡抗猪瘟高免卵黄抗体的研制

猪瘟是由猪瘟病毒引起的一种急性、热性、接触性传染病。近年来，尽管普遍对猪群用猪瘟兔化弱毒苗进行免疫接种，但免疫失败的现象时常发生，给养猪业造成了很大的经济损失。为了找到一个治疗猪瘟的经济有效方法，我们开展了鸡抗猪瘟高免卵黄的研制。１材料与方法１．１试...     

某猪场猪瘟强毒、弱毒抗体水平的检测

应用猪瘟单克隆抗体纯化酶联免疫吸附试验方法，对河南某猪场47份母猪血清进行猪瘟抗体检测，有5份血清呈猪瘟强毒抗体阳性，阳性率为10.6%，猪瘟弱毒抗体阳性率为95.7%，其群体保护率为93％。在9份仔猪血清中，猪瘟强毒抗体为阴性，猪瘟弱毒抗体阳性率为22％，只有17％的群体保护率。检测结果表明：该猪场可能有猪瘟强毒感染。     

金堂县农村散养猪猪瘟保护力的监测试验

用猪瘟Dot-EILSA诊断试剂盒测定农村散养猪猪瘟血清效价，采用传统方法检查猪瘟预防注射密度为100％的样17份，效价在1：80及以上12份，猪瘟保护力70．6％。     

大剂量猪瘟单苗紧急免疫控制猪瘟蔓延

猪瘟是由猪瘟病毒引起的一种急性、热性、高度接触性传染病，传染快、死亡率高。如果未经猪瘟疫苗防疫注射，往往引起全群大面积猪只发病。我市大部分规模养猪场（户）都是从外地引进苗猪后进行育肥性饲养，由于对引进苗猪的基础防疫不了解，引进后即按标准注射１头份猪瘟单苗或１头份二联苗，使引进苗猪发生猪瘟蔓延时有发生。为研究引进苗猪猪瘟控制的有效方法，笔者在吴县市浦庄镇兽医站商品猪场对引进猪只进行了猪瘟防疫试验，现将结果报告如下。１　材料与方法１－１　试验用疫苗猪瘟单苗，猪瘟、猪丹毒二联苗均系南京药械厂生产，每瓶…     

应用ELISA试验检测猪瘟免疫抗体

猪瘟在我省流行比较严重，曾造成较大的经济损失，从1988年后全省采取每年春秋两防，并随时补针的免疫措施，使我省的猪瘟疫情得到了全面的控制。但是，我省由外地引入仔猪的猪瘟病例时有发生。为了掌握我省猪瘟免疫状况，海南省动物疫病诊断中心于2005年7月应用猪瘟ELISA试验方法在省内6个市县进行了猪瘟免疫抗体检测。     

Disease and destiny-mystery and mastery

When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures.     

奶牛肢蹄病及其防治

简介常见奶牛肢蹄病种类及其症状,从日粮营养、运动场地面结构、环境卫生、饲养管理、遗传育种、疾病管理等方面简析奶牛肢蹄病的病因,并提出相应的预防措施。     

Foot and mouth disease and bluetongue: differences and similarities

On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures.     

德国荷兰畜禽产品质量安全交流与启示

2018年岁末,笔者有幸赴德国和荷兰,就畜产品质量安全控制及检测技术等进行了短期交流,收获颇丰,现具体介绍如下。一、交流情况德国面积35.73万平方千米,人口约8175.2万,是欧盟人口最多的国家,农业发达,机械化程度很高。德国的畜牧业以猪、牛、羊和禽类为主,畜牧业产值占农业生产总值的61%。德国虽然农业比重很小,但却是有机农业运动最早的发起国,也是目前世界上有机农产品生产与消费大国。     

畜禽物种多样性及其保护和利用

文章就我国畜禽物种多样性资源的现状、保护和利用等方面进行阐述,为生态脆弱的喀斯特地区畜牧业可持续发展提供参考。     

马的咽气癖及其防制措施研究进展

在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。     

牛羊焦虫病诊断与防治技术

近几年来,辽宁省牛羊饲养业迅速发展,焦虫病的危害越来越明显,已成为危害牛羊的主要疾病之一。为了提高牛羊焦虫病的防治水平,保证我省牛羊饲养业的健康发展,笔者在参阅文献和同行们诊疗经验的基础上,结合自己的治疗实践,详细阐述了我省牛羊焦虫病近几年的流行特点和诊断防治方法。     

国内外蛋氨酸与赖氨酸市场分析与展望

近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2％的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。