Changing trends in infectious bovine rhinotracheitis in Great Britain

The incidence and severity of infectious bovine rhinotracheitis in Great Britain increased markedly in the mid to late 1970s. Data available in MAFF records have been used to analyse the changing trends in disease incidence and serological prevalence, with particular emphasis on the most recent decade.     

雏鸭胸腺发育的增龄变化

通过对1～49日龄雏鸭胸腺组织学发育的增龄变化观察以及胸腺绝对重量、胸腺指数和外周血T淋巴细胞а-醋酸萘酯酶阳性率的测定,研究了雏鸭胸腺发育规律。结果表明：1～14日龄雏鸭胸腺生长较为缓慢;21～28日龄胸腺生长十分迅速,到28日龄时雏鸭胸腺中大部分T淋巴细胞已分化发育成熟,雏鸭胸腺已形成能有效产生细胞免疫的组织结构基础;35～49日龄胸腺发育已趋于稳定。文中对胸腺小体的形态进行了详细的描述,并对其功能进行了探讨。     

Heifer mastitis and dry-cow mastitis: A bacteriological survey in Sweden

A bacteriological investigation was performed on 145 heifers and 55 dry-cows with clinical mastitis during July 1976–June 1977. Areas in Sweden from which heifer and dry-cow mastitis has been reported as a problem were included. Mastitis occurred more frequently in the Swedish Fresian breed (SLB) than in the Swedish Red and White breed (SRB) of dairy cattle. C. pyogenes was isolated in pure culture from 15% and 4%, respectively, of the cases of heifer and dry-cow mastitis from which this bacterium could be isolated. Corresponding figures for pure culture of P. indolicus, micro-aerophilic cocci and S. dysgalactiae were 2% and 0%, 0% and 0% and 44% and 35%, respectively. The bacteria most frequently isolated together with C. pyogenes in the heifer material were P. indolicus (69%), the microaerophilic cocci (59%) and S. dysgalactiae (31%). Corresponding data for the dry-cow material were 92%, 35% and 50%, respectively. Thus, C. pyogenes was the causative agent of heifer and dry-cow mastitis in only about 40% of the mastitis cases investigated, and the most characteristic feature of these forms of mastitis involving C. pyogenes was mixed infection.     

Methods of diagnosing subclinical mastitis in dairy cow mastitis control programs

The number of somatic cells and the isolation of the causative agents of mastitis in quarter, composite, bucket, and bulk tank samples of cow's milk was determined four times during a six-month period. The number of somatic cells in milk samples indicated a degree of mastitis infection and was influenced neither by the year season nor by the length of lactation. At a repeated examination of 28 dairy cows an increased number of somatic cells in milk was found once in 68 udder quarters and with three successive samplings only in 21 quarters. The etiological agents of mastitis were detected once in 31 quarters and three times in succession only in five quarters. The number of cows positive by the number of cells in quarter samples of milk increased from 52.9-58.8% at a single examination to as much as 100% at four examinations. The etiological agents of mastitis were isolated in a single examination in 17.6% of cows and at four examinations in 58.8% of cows. The composite and bucket samples of milk containing 200 to 300 thousand cells per ml are recommended to be considered as mastitis-positive: in 68 to 78% they came from cows having more than 500 thousand cells per ml at least in one quarter sample. The number of cells in a bulk sample was in correlation with the percentage of cows having a positive NK-test (similar to CMT) and positive isolation of S. agalactiae from quarter milk samples.(ABSTRACT TRUNCATED AT 250 WORDS)     

Coliform mastitis

Gram-negative bacteria that commonly cause bovine mastitis are classified as environmental pathogens. The point sources of coliform bacteria that cause infections include bedding materials, soil, manure and other organic matter in the environment of cows. Rates of coliform mastitis increase during climatic periods that maximize populations in the environment. The portal of entry into the mammary gland for Gram-negative bacteria is the teat canal. Once in the gland, bacteria must utilize available substrates in the mammary secretion to replicate and evade host defenses. Rates of coliform mastitis are greater during the transitional phases of the non- lactating period than during lactation. The ability to infect the non-lactating gland is directly related to the ability of bacteria to acquire iron from the mammary secretion. The primary host defense against coliform mastitis during lactation is the elimination of bacteria by neutrophils migrating into the gland in response to inflammation. Damage to the host is mediated by the release of endotoxin. The severity and duration of clinical signs associated with coliform mastitis are reduced by the use of core-antigen bacterins.     

Production effects related to mastitis and mastitis economics in dairy cattle herds

Mastitis is the most prevalent production disease in dairy herds world-wide and is responsible for several production effects. Milk yield and composition can be affected by a more or less severe short-term depression and, in case of no cure, by a long-acting effect, and, sometimes, an overlapping effect to the next lactation. Summary values in the literature for losses of milk production were proposed at 375 kg for a clinical case (5% at the lactation level) and at 0.5 kg per 2-fold increase of crude SCC of a cow. Due to the withdrawal period after treatment, composition changes in milk can almost be neglected in economic calculations. Lethality rate for clinical mastitis is very low on the average, while anticipated culling occurs more frequently after clinical and subclinical mastitis (relative risk between 1.5 and 5.0). The economics of mastitis needs to be addressed at the farm level and, per se, depends on local and regional epidemiological, managerial and economic conditions. To assess the direct economic impact of mastitis, costs (i.e. extra resource use) and losses (i.e. reduced revenues) have to be aggregated. To support decision making for udder health control, it is necessary to use a marginal approach, based on the comparison of the losses avoided and the additional costs of modified plans, compared to the existing ones.     

Mycoplasma mastitis in dairy cattle

The most important characteristics of Mycoplasma mastitis on dairy farms are described, based on two case studies. Clinical symptoms, diagnostics, epidemiology, and a plan of action are presented. In the herds investigated, Mycoplasma mastitis was characterized by multiple affected quarters unresponsive to treatment with antibiotic and/or anti-inflammatory agents. Most striking were a sandy sediment, brown colouring, and rice-like structure of the milk of affected animals. Clinical symptoms differed in the two affected herds. Diagnosis was based on bacteriological investigation of samples of milk and synovial fluid taken from infected cows. Affected animals were culled immediately, and the herds were monitored by repeated testing of bulk milk samples. It was concluded that a consequence of the increasing size of cattle herds in the Netherlands is that subclinical/clinical Mycoplasma mastitis may be diagnosed more frequently than in the past. In the case of Mycoplasma mastitis, farmers and veterinary practitioners are advised to draw up a plan of action together, incorporating aspects such as diagnostics at cow level, direct culling of affected animals, hygiene during milking, including post-milking teat disinfection, and routine monitoring of bulk milk. Unpasteurized milk should not be given to calves.