Optimization of production of nucleopolyhedrovirus ofHelicoverpa armigera throughout larval stages

Studies were carried out to optimize production of nucleopolyhedrovirus of American bollworm,Helicoverpa armigera (Hübner), by treating larvae individually with an inoculum dose that allowed maximal larval growth and also gave the highest occlusion bodies (OB) yield/larva. The maximum virus yield of 12.2x 10⁹ OB/larva was obtained when 6-day-old larvae were fed individually with a dose of 1 x 10³ OB. Topical spiracular treatment of larvae as old as 8 days with 10 μ of 2x 10⁷ OB ml^-1 gave the highest yield, of 15.2x 10⁹ OB from 13-day-old larvae, of 12.8x 10⁸ OB from prepupae and of 1.49x 10⁸ OB from pupae at the time of their death. These studies showed that dietary inoculum is the best route for 6-day-old larvae and topical spiracular treatment is the best for 8-day-old larvae.     

Laboratory evaluation of the insect growth regulator lufenuron againstHelicoverpa armigera on cotton

An insect growth regulator (IGR), lufenuron (Match 5EC), was tested for its toxicity toHelicoverpa armigera on cotton. Potency of the IGR against the larval stage of the pest was demonstrated with respect to larval instars; the LC₉₀ values of 1st, 2nd, 3rd, 4th and 5th instar larvae were 5.63, 7.89, 8.03, 11.39 and 14.76 mg a.i.l ⁻¹, respectively. However, different larval instars did not differ significantly with respect to LC₅₀ and LC₁₀. IGR-treated larvae had swollen heads and were significantly smaller (1.5–2.3 mm) than the untreated control (2.9 mm). Larval weight was significantly reduced from 190 mg in the control to 50–70 mg in the lufenuron treatment. IGR treatment in the larval stage significantly affected both pupal length and pupal weight. Pupal duration of the test insect was significantly extended by IGR treatment. Pupal deformities, including an inability to shed the last larval skin and formation of larval-pupal intermediates, occurred following treatment. A significant reduction in adult emergence was recorded. In addition, abnormalities in the form of development of cavities in the forewings of adult were evident. A significant decline in fecundity was noted in the studies. http://www.phytoparasitica.org posting Feb. 3, 2003.     

Ovipositional response ofHelicoverpa armigera to different cotton genotypes

The ovipositional response ofHelicoverpa armigera (Hübner) to different cotton varieties and species was studied under caged conditions. Maximum oviposition (51.6 eggs/female) was recorded on LH 900, a variety ofGossypium hirsutum, and minimum oviposition (3.0 eggs/female) on G 27, a variety ofG. arboreum. Oviposition, in general, was low (3 to 6.5 eggs/female) onarboreum cottons as compared withhirsutum. Of the number of factors found to affect the oviposition ofH. armigera, the trichome length on the upper surface of the leaf, rather than the density, was positively correlated (r = 0.687*). Among different months in the cropping season, oviposition was maximum (552.7) during April. Oviposition was higher on leaves than on other plant parts.     

气候变化背景下玉米棉铃虫消长动态预测及影响因素研究

采用甘肃省武威市农技中心植保站1999—2008年棉铃虫观测资料及武威市气象局观测站1953—2010年气象资料,利用统计学方法中的作物生长模型、主成分分析方法,建立了棉铃虫种群增长模型和气象预报预测模型,分析了气候变暖特征及对棉铃虫种群增长的影响。结果表明：玉米棉铃虫百株卵量周年增长动态分缓慢增长、快速增长、减速增长三个时期,快速增长期出现在7月下旬末至8月上旬（玉米吐丝期）;建立了棉铃虫卵始见期、卵高峰期、高峰期卵量、高峰期虫量气候预测模型,预测准确率78%～89%,可用于棉铃虫预报业务应用;揭示了气候变化对玉米棉铃虫发生发展的影响：棉铃虫越冬期（12—2月）最低气温每10年增温0.58℃,暖冬利于棉铃虫安全越冬,越冬基数增加,成活率提高;繁殖期（5—9月）≥10℃积温每10年增加133℃。热量条件改善,有利于棉铃虫种群增长加快、繁殖代数增加、危害期延长;气候极端事件发生的不确定性,对棉铃虫的发生发展将起到明显的抑制作用。     

Effects of a primitive cotton accession on feeding ofspodoptera littoralis andhelicoverpa armigera and on oviposition ofBemisia tabaci

A primitive cotton(Gossypium hirsutum L.) accession, V-64, from the Caribbean Islands was bioassayed for antibiosis against pests of this crop in comparison with the commercial strains Acala SJ-2 and Pima S-5. V-64 reduced weight gain and leaf consumption ofSpodoptera littoralis andHelicoverpa armigera, and significantly reduced oviposition ofBemisia tabaci. The leaf pigment glands in V-64 were larger and more abundant than in the commercial strains, and the feeding ofH. armigera on these glands was avoided, indicating that the glands probably constituted the major resistance character in V-64. Contribution from the Agricultural Research Organization. No. 3187-E, 1991 series.     

甲氧虫酰肼对棉铃虫解毒酶活力的亚致死效应研究

采用亚致死剂量(LC40浓度)的甲氧虫酰肼处理棉铃虫抗甲氧虫酰肼种群(R)和同源敏感种群(S)3龄初幼虫48 h,测定了3～6龄期幼虫体内多功能氧化酶(MFO)、酯酶(EST)和谷胱甘肽S-转移酶(GSTs)的比活力变化。结果发现:经亚致死剂量甲氧虫酰肼处理后,S种群EST比活力除4龄幼虫外均有所升高,且仅在3龄期升高明显;GSTs比活力在5龄幼虫体内显著升高,其他龄期无明显变化;MFO比活力在6龄幼虫体内表现为降低,其他龄期无显著变化。对于R种群,3龄和6龄幼虫体内EST比活力显著降低,各龄期的GSTs和MFO比活力均显著降低。空白对照R种群3～6龄期幼虫的GSTs比活力均高于S种群;EST比活力仅3龄幼虫显著高于S种群;MFO的比活力仅5龄幼虫显著高于S种群。研究表明:棉铃虫对甲氧虫酰肼的抗性与GSTs比活力增大关系较为密切;LC40浓度的甲氧虫酰肼可诱导敏感种群EST和GSTs活力升高,而使抗性种群3种酶的活力受到抑制。     

蠋蝽对棉铃虫幼虫的捕食功能反应

为明确蠋蝽Arma custos对棉铃虫Helicoverpa armigera幼虫的生物防控潜能,在室内条件下测定蠋蝽5龄若虫和雌、雄成虫对棉铃虫3龄幼虫的捕食能力以及不同蠋蝽密度对自身捕食作用的影响。结果显示:蠋蝽5龄若虫和雌、雄成虫对棉铃虫3龄幼虫的捕食功能反应均符合HollingⅡ模型。蠋蝽5龄若虫对棉铃虫3龄幼虫的瞬时攻击率、处理时间和日最大捕食量分别为0.829、0.024 d和41.667头;蠋蝽雌成虫对棉铃虫3龄幼虫的瞬时攻击率、处理时间和日最大捕食量分别为0.796、0.020 d和50.000头;蠋蝽雄成虫对棉铃虫3龄幼虫的瞬时攻击率、处理时间和日最大捕食量分别为0.752、0.021 d和47.619头。蠋蝽5龄若虫和雌、雄成虫对棉铃虫3龄幼虫的搜寻效应均随猎物密度的增加而逐渐降低。当棉铃虫幼虫密度固定时,单头蠋蝽的日捕食量随蠋蝽密度的增加而逐渐降低,蠋蝽对棉铃虫的捕食作用受到自身密度的干扰,5龄若虫和雌、雄成虫的干扰系数分别为0.548、0.702和0.772。表明蠋蝽5龄若虫及雌、雄成虫均对棉铃虫3龄幼虫具有一定的防控能力,且成虫对棉铃虫幼虫的防控能力大于5龄...     

寄主植物调控棉铃虫种群对气候变暖的不对称响应

为明确全球气候变暖对不同寄主植物上棉铃虫Helicoverpa armigera种群发生的影响,通过收集2008—2019年全国范围、华北地区、华东地区、西北地区及华中地区7种寄主植物(小麦、玉米、大豆、棉花、花生、蔬菜和其他粮食作物)上棉铃虫成灾面积及各地区平均温度数据,采用单因素方差分析法及广义线性模型分析不同寄主植物上棉铃虫种群的发生程度。结果显示,玉米、大豆、花生和蔬菜寄主植物上棉铃虫成灾面积显著增加,棉花上棉铃虫成灾面积显著降低。玉米(全国范围、华北地区)、花生(全国范围、华中地区)及蔬菜(华东地区)上棉铃虫成灾面积随着温度距平的升高而显著增加;但棉花上棉铃虫成灾面积随着温度距平的升高而显著降低。如全国范围内,在玉米和花生上,2014—2019年棉铃虫成灾面积均显著高于2008—2013年,其增加比率分别为24.0%和34.3%;在棉花上,2014—2019年棉铃虫成灾面积显著低于2008—2013年,其降低比率为62.0%。表明全球气候变暖显著增加了玉米、花生及蔬菜上棉铃虫成灾面积,而棉花上棉铃虫成灾面积的降低主要受转Bt基因棉花种植的影响。     

蓝桉叶片挥发物对棉铃虫产卵选择行为的影响

为筛选棉铃虫Helicoverpa armigera的产卵驱避剂,利用顶空动态吸附法和气相色谱质谱联用技术分析其非寄主植物蓝桉Eucalyptus globulus叶片挥发物的主要成分和相对含量,通过气相色谱-触角电位联用技术研究抱卵雌蛾对蓝桉叶片挥发物的电生理反应,并测定其电生理活性组分对抱卵雌蛾产卵选择行为的影响。结果表明,蓝桉叶片挥发物中共鉴定出39种物质,以萜烯类物质为主,约占挥发物总量的97.47%,其中含量最高的4种物质为α-蒎烯、反-罗勒烯、1,8-桉叶素和柠檬烯,分别占挥发物总量的44.65%、10.55%、8.16%和7.37%。能够引起棉铃虫抱卵雌蛾电生理反应的物质有12种,分别是α-蒎烯、β-蒎烯、β-月桂烯、α-水芹烯、柠檬烯、1,8-桉叶素、反-罗勒烯、4-异丙烯基甲苯、4,8-二甲基-1,3,7-壬三烯、1,3,8-对薄荷三烯、香橙烯和α-法尼烯。α-水芹烯在0.1μg剂量、α-蒎烯和α-法尼烯在1μg剂量时对抱卵雌蛾均表现出显著的产卵引诱作用;柠檬烯在100μg剂量时表现为显著的产卵驱避作用,其余剂量处理下均失去引诱或驱避效果;4-异丙烯基甲苯在1、10和100μg剂量以及1,8-桉叶素在10μg和100μg剂量时均表现为显著的产卵驱避作用。表明4-异丙烯基甲苯和1,8-桉叶素可作为棉铃虫产卵驱避剂备选物质进行研究和开发。     

重组增效蛋白对Bt和氯氰菊酯防治棉铃虫的增效作用

前期在大肠杆菌中表达得到具有显著生物活性的含粉纹夜蛾颗粒体病毒增效蛋白C端818个氨基酸的融合蛋白P96。以毒饲料法研究P96对苏云金芽孢杆菌和氯氰菊酯杀棉铃虫敏感、抗性品系的增效作用。结果表明：P96对Bt杀棉铃虫敏感、低抗、中抗品系的增效比分别为3．34、4．72和9．82；对氯氰菊酯杀棉铃虫敏感、低抗、中抗品系的增效比分别为2．53、3．38和6．24。随棉铃虫抗性升高，P96的增效作用愈为明显。单对汰选是快速选育棉铃虫抗Bt品系的有效方法。     

Molecular cloning and mRNA expression of a ryanodine receptor gene in the cotton bollworm, Helicoverpa armigera

Ryanodine receptors (RyRs) are the targets of novel diamide insecticides. The cotton bollworm, Helicoverpa armigera, is one of the most important cotton pests in the world. In this study, we report the full-length RyR cDNA sequence (named as HaRyR) of H. armigera. The 16,083-bp contiguous sequence encoded 5, 142 amino acid residues, which shares 80% and 78% overall identities with its homologues in Nilaparvata lugens (NlRyR) and Drosophila melanogaster (DmRyR), respectively. All hallmarks of RyR proteins are conserved in the HaRyR, including the GXRXGGGXGD motif conserved in the Ca²⁺ release channels and four copies of RyR domain unique to RyR channels. The previously identified seven lepidopteran-specific RyR residues were also found in HaRyR (N⁴⁹⁷⁷, N⁴⁹⁷⁹, N⁴⁹⁹⁰, L⁵⁰⁰⁵, L⁵⁰³⁶, N⁵⁰⁶⁸ and T⁵¹¹⁹). An amino acid sequence alignment showed that the N-terminal region of HaRyR (residues 188–295) shared high sequence identity with NlRyR (94%) and DmRyR (92%), and moderate sequence identity (47–50%) with three rabbit RyR isoforms, while the short segment of the C-terminal transmembrane region of HaRyR (residues 4632–4676) exhibited moderate sequence identity with NlRyR (69%) and DmRyR (67%), and low sequence identity (19–28%) with three rabbit RyR isoforms. In addition, expression analysis of HaRyR revealed that the mRNA expression level in eggs was significantly lower than in third instar larvae, pupae and adults, and anatomical regulation of HaRyR expression was also observed with the highest expression level in head compared with thorax and abdomen. Our results lay a foundation for comprehensive structural and functional characterization of HaRyR and for understanding of the molecular mechanisms of toxicity selectivity of diamide insecticides among different species.     

棉铃虫类肌钙蛋白基因的克隆、原核表达纯化及其时空表达谱分析

为探索棉铃虫Helicoverpa armigera(Hübner)类肌钙蛋白基因HaCal的生理功能及其所编码蛋白的生物特性,采用PCR方法克隆了HaCal的开放阅读框(open reading frame,ORF)序列,借助在线软件对其进行生物信息学分析,通过原核表达技术诱导表达和纯化其编码的蛋白,并通过实时荧光定量PCR技术分析其在棉铃虫不同发育龄期和组织中的表达量。结果显示,HaCal的ORF序列长度为564 bp,编码187个氨基酸,理论分子量为20.52 kD,理论等电点为7.64。其保守结构域与Calponin家族一致,但不含跨膜区和信号肽,系亲水性蛋白。原核表达结果显示,融合蛋白大小与理论值一致,纯化获得了纯度较高的目的蛋白。HaCal在棉铃虫幼虫不同发育阶段和不同组织中均有表达,在6龄幼虫体内表达量最高,是1龄幼虫的2.34倍;在5龄幼虫中肠中表达量最高,是头部的257.14倍。表明棉铃虫类肌钙蛋白基因HaCal可能与棉铃虫的生长发育过程密切相关。     

棉铃虫V-ATP酶亚基A基因克隆及表达谱分析

为探索棉铃虫Helicoverpa armigera液泡型ATP酶(vacuolar-type proton ATPase,V-ATP酶)亚基A对棉铃虫生长发育的影响及其在Bt杀虫机制中的作用,采用PCR结合RACE技术克隆了棉铃虫V-ATP酶亚基A基因序列,通过实时荧光定量PCR技术测定了其在棉铃虫不同发育历期和幼虫肠道不同组织中的表达量;并比较了4龄幼虫取食含Cry1Ac蛋白饲料后中肠中该基因表达量的变化。结果显示,棉铃虫V-ATP酶亚基A基因全长2 578 bp(Gen Bank登录号KP090287),开放阅读框1 863 bp,编码621个氨基酸。V-ATP酶亚基A高度保守,不同物种间氨基酸序列同源性大于90%。V-ATP酶亚基A在棉铃虫整个生育期都有表达,在4龄幼虫体内表达量最高,是卵期的3.00倍;在幼虫肠道不同组织中,中肠中表达量最高,是后肠中的2.65倍。4龄棉铃虫幼虫取食含Cry1Ac蛋白的人工饲料后,中肠V-ATP酶亚基A的表达受到抑制,表达量为对照的0.39~0.81倍。表明V-ATP酶亚基A基因可能参与棉铃虫的生长发育和代谢过程,并可能与抵御Cry1Ac的毒杀作用有一定关系。     

转cry1Ab/cry2Aj、cry1Ab/vip3DA玉米对棉铃虫、甜菜夜蛾和斜纹夜蛾的抗虫性评价

为评估转cry1Ab/cry2Aj、cry1Ab/vip3DA玉米对棉铃虫Helicoverpa armigera(Hübner)、甜菜夜蛾Spodoptera exigua(Hübner)和斜纹夜蛾Prodenia litura(Fabricius)的抗虫性,在室内测定了3个转cry1Ab/cry2Aj玉米品系和1个转cry1Ab/vip3DA玉米品系对3种害虫幼虫存活和生长发育的影响,研究了该系列Bt玉米不同组织器官对害虫的杀虫活性和控制效果。结果显示,棉铃虫初孵幼虫取食各品系Bt玉米叶片96h后死亡率为87.50%~90.00%,取食花丝和雌穗的幼虫96h后几乎全部死亡;甜菜夜蛾初孵幼虫取食各品系Bt玉米叶片、花丝和雌穗168h后死亡率为22.50%~68.33%,幼虫的生长发育受到明显抑制,体重抑制率达85.00%~95.00%;斜纹夜蛾初孵幼虫取食各品系Bt玉米叶片、花丝和雌穗96 h后死亡率显著高于非转基因亲本对照,168h后幼虫死亡率达90.00%以上。研究表明,转cry1Ab/cry2Aj和cry1Ab/vip3DA玉米品系对棉铃虫和斜纹夜蛾的初孵幼虫表现出较好的抗性,可以作为转多基因抗虫玉米育种的备选材料。     

棉铃虫YTHDF1基因的表达模式及在核型多角体病毒侵染中的作用

为明确N6-甲基腺苷（N6-methyladenosine,m⁶A）修饰在棉铃虫核型多角体病毒（Helicoverpa armigera nucleopolyhedrovirus,HearNPV）侵染棉铃虫中的作用,根据基因组和转录组数据,对棉铃虫m⁶A结合蛋白基因YTHDF1（YTH domain-containing family protein 1）进行鉴定和分析,利用实时荧光定量PCR技术检测棉铃虫YTHDF1基因的时空表达模式、HearNPV处理后的表达变化情况以及RNA干扰效率,并调查该基因下调后对HearNPV复制及感染HearNPV棉铃虫死亡情况的影响。结果显示,棉铃虫YTHDF1基因开放阅读框全长为2 019 bp,编码672个氨基酸,含有1个保守的YTH结构域,其氨基酸序列与斜纹夜蛾Spodoptera litura同源物序列一致性达87.52%。YTHDF1基因在棉铃虫不同发育阶段均有表达,其中在进入5龄期24 h幼虫中的表达量最高,在2龄取食期幼虫中的表达量最低。YTHDF1基因的空间表达谱呈现一定的组织特异性,在幼虫血细胞和成虫头部的表达量最高。HearNPV侵染棉铃虫后YTHDF1基因上调表达,经RNA干扰使该基因下调后显著抑制了HearNPV多角体蛋白基因polyhedrin的表达并延迟了感染HearNPV棉铃虫的死亡时间。表明YTHDF1基因在HearNPV侵染棉铃虫的过程中发挥着重要作用。     

棉铃虫四个丝氨酸蛋白酶抑制剂基因的克隆与表达分析

为明确棉铃虫Helicoverpa armigera丝氨酸蛋白酶抑制剂（serine protease inhibitor,serpin）的种类及其表达特性,利用PCR技术克隆棉铃虫的serpin基因,使用生物信息学软件预测其结构并进行系统进化分析,采用实时荧光定量PCR（real time quantitative PCR,RT-qPCR）技术比较serpin基因在棉铃虫不同发育阶段和组织中的表达量及取食Cry1Ac后其表达量的变化。结果表明,共获得serpin-a、serpin-b、serpin-c、serpin-e四个棉铃虫serpin基因,全长为1 119~1 254 bp,编码373~418个氨基酸,均包含一段具有反应中心环的保守结构域,且与斜纹夜蛾Spodoptera litura、草地贪夜蛾S.frugiperda等鳞翅目昆虫serpin的同源性较高。serpin-a和serpin-e在棉铃虫4龄幼虫期的表达量最高,serpin-b和serpin-c分别在成虫期和蛹期表达量最高。serpin-a在中肠和围食膜中表达量最高,serpin-b在头、中肠和表皮中表达量最高,serpin-c在头部表达量最高,serpin-e在中肠和血淋巴中的表达量显著高于其他组织。棉铃虫取食低浓度Cry1Ac后,中肠的serpin-b和serpin-e的表达量显著增加。推测不同serpin基因在棉铃虫不同发育时期和组织中可能发挥不同的作用,其中serpin-b和serpin-e可能参与棉铃虫对Cry1Ac的解毒过程。     

我国棉铃虫对Bt蛋白Cry1Ac抗性现状及分子机制研究进展

棉铃虫Helicoverpa armigera是一种全球性的重要农业害虫,主要为害棉花、玉米和大豆等作物。长期种植单价Bt棉花（表达Cry1Ac蛋白）会使棉铃虫田间种群承受单一、持续的选择压力,必然会导致棉铃虫对Cry1Ac的抗性发生演化。该文概述我国棉铃虫田间种群对Cry1Ac的抗性现状、自然庇护所对棉铃虫Cry1Ac抗性演化的延缓作用以及棉铃虫对Cry1Ac抗性的遗传多样性,并对今后我国关于棉铃虫Bt抗性的治理对策进行了展望。     

基于线粒体全基因组解析华北地区棉铃虫种群遗传结构

为明确我国华北地区棉铃虫种群间遗传分化,通过高通量测序测定华北地区河北省廊坊市、河南省新乡市和山东省烟台市27份棉铃虫线粒体全基因组,并分析这3个市棉铃虫种群的遗传结构。结果表明,华北地区棉铃虫线粒体基因组全长15 345~15 375 bp,长度变异主要由基因间隔区的A+T富集区造成,所有样品的13个蛋白编码基因在长度上均无差异,总群体间遗传分化很小,遗传分化指数为0.025,基因流水平很高,为19.46。27份样品被聚为亚型I和亚型II两个遗传分支,但未形成明显的地理结构,其中亚型II比例较低,仅包含河北省和山东省的4份样品。表明我国华北地区棉铃虫种群存在线粒体亚型分化,不同区域种群之间存在广泛的基因交流,没有形成谱系遗传结构,推测棉铃虫在华北地区存在高迁飞现象。     

转Bt基因棉田棉铃虫对气候变暖与集约化种植的不对称响应

为明确全球气候变暖及农业集约化对转Bt基因棉田棉铃虫Helicoverpa armigera种群发生的影响,通过收集1987—2017年我国山东、河南及河北3省的棉花种植面积、棉铃虫发生面积及年平均温度,采用单因素方差分析法及广义线性模型分析比较转Bt基因棉花推广前后棉田棉铃虫种群的发生程度,分析棉铃虫的为害指数。结果显示,转Bt基因棉花推广前,山东、河南和河北3省的棉铃虫在严重发生年份的为害指数较轻微发生年份均显著增加,分别增加了1.01倍、2.03倍和69.33%;温度距平具有同样的趋势,严重发生年份的温度距平较轻微发生年份分别显著增加了1.91倍、2.33倍和2.29倍。转Bt基因棉花推广后,山东、河南和河北3省的棉铃虫在严重发生年份的为害指数较轻微发生年份仍显著增加,分别增加了1.47倍、1.92倍和1.25倍;温度距平表现出相反的趋势,轻微发生年份的温度距平较严重发生年份分别增加了2.00倍、1.94倍和2.01倍;而河南省和河北省在棉铃虫严重发生年份的棉花种植面积较轻微发生年份分别显著增加了75.94%及38.92%。表明转Bt基因棉花种植后显著改变了棉铃虫对全球气候变暖的响应,气候变暖降低了棉铃虫的为害指数,而棉花集约化种植增加了棉铃虫的为害指数。非转基因棉田的棉铃虫受自然因素的影响,转Bt基因棉田的棉铃虫受自然及人为因素的双重影响。     

棉铃虫表皮蛋白基因CP22和CP14的表达特征及其对甲氧虫酰肼的响应

为揭示棉铃虫Helicoverpa armigera表皮蛋白（cuticular protein,CP）基因在其生长发育及应对药剂胁迫中的作用,克隆棉铃虫2个CP基因CP22和CP14,利用实时荧光定量PCR技术分析其在不同发育阶段和不同组织中的相对表达量,并于显微镜下观察甲氧虫酰肼亚致死剂量处理后棉铃虫3龄幼虫的表皮形态,并用实时荧光定量PCR技术测定药后CP22和CP14基因的相对表达量。结果显示,CP22和CP14的开放阅读框全长分别为570 bp和393 bp,分别编码189个和130个氨基酸;CP22和CP14都具有1个几丁质结合域,属于CPR家族RR-1亚类;CP22和CP14基因均在棉铃虫5龄幼虫表皮中表达水平高;这2个基因在棉铃虫幼虫期的表达水平高于在卵期、蛹期和成虫期的表达水平,且在4龄幼虫体内表达量最高,在蜕皮后随着日龄的增加表达量逐渐降低;甲氧虫酰肼处理后棉铃虫3龄幼虫表皮黑化、皱缩,发生蜕皮异常,显微观察显示其内外表皮分离,真皮细胞解体;甲氧虫酰肼处理后24 h和48 h,棉铃虫CP22和CP14基因的相对表达量显著上调。表明棉铃虫CP22和CP14基因参与棉铃虫幼虫蜕皮,并且响应甲氧虫酰肼胁迫,可作为防治棉铃虫的潜在靶标基因。