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《中国畜牧兽医文摘》2012,(8)
研究兔白介素-6基因真核重组质粒(pcDNA-IL-6)对兔瘟组织灭活苗免疫效果的影响。本文构建了真核重组质粒pcDNA-IL-6,并将其与兔瘟组织苗共同免疫家兔,以兔瘟组织灭活苗和质粒pcDNA3.1(+)作对照,用血凝抑制试验检测家兔抗RHDV特异性抗体水平。结果:在免疫后的7~70d,兔瘟组织灭活苗与真核重组质粒pcDNA-IL6注射免疫组抗体水平高于只免疫兔瘟组织灭活苗组,差异均显著p0.05或极显著p0.01。真核重组质粒pcDNA-IL-6对兔瘟组织灭活苗表现出来明显的免疫佐剂效应。 相似文献
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牦牛ETEC灭活疫苗的研制及免疫效力试验 总被引:4,自引:0,他引:4
制备了耗牛ETEC铝胶灭活苗、油佐剂苗和蜂胶灭活苗。分别使用3种佐剂灭活苗进行对比试验。经抗体监测表明在免疫后7天,蜂胶苗产生的抗体效价最高(2^7),随着时间的推移,油苗抗体儿价最高(2^9-10),于免疫后29日,使用ETEC进行攻毒,免疫兔全部保护,对照兔全部死亡。证明牦牛ETEC3种佐剂灭活疫苗均能有效保护家兔免受ETEC攻击。 相似文献
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采用对本地区具有良好免疫原性的兔病毒性出血症强毒株和巴氏杆菌菌株,研制出常规二联灭活苗和蜂胶二联灭活苗.用血清学方法对常规二联灭活苗和蜂胶二联灭活苗免疫后兔病毒性出血症抗体的检测.结果表明:常规二联灭活苗免疫后7 d HI抗体可达5.0log2,15 d明显上升,达5.510g2,30 d达到高峰,可达8.0log2,;而蜂胶二联灭活苗在免疫后7 d HI抗体可达5.0log,15d明显上升,达5.5log2以上,20 d显著上升9.25log2以上,30 d达到高峰,可达11.0log2.说明蜂胶提高抗体效价作用明显,可显著增强免疫效果. 相似文献
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经血凝抑制和攻毒试验结果表明,在含有相同抗原量制备的疫苗,兔瘟油乳剂灭活疫苗比兔瘟组织灭活苗免疫后血清HI抗体效价高3.25 ̄6.0log2,即使油乳剂灭活疫苗仅为组织灭活苗四分之一的抗原量,免疫后血清HI抗体效价还高1log2。油乳剂灭活苗免疫兔第50天其血清HI抗体未见下降,而组织灭活苗免疫后第26天已开始下降。虽然接种二种剂型的疫苗攻毒后均具有100%的保护率,但血清HI抗体效价消长情况证明 相似文献
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几种中药成分的免疫增强活性及其作用效果 总被引:20,自引:0,他引:20
测定了黄芪多糖等9种中药成分对正常小鼠脾和外周血淋巴细胞增殖反应的影响,并观察了这些中药成分对兔瘟组织灭活疫苗的增强免疫效果。结果表明,人参皂甙、黄芪多糖、淫羊藿多糖、当归多糖和蜂胶黄酮能显著增强伴刀豆球蛋白(ConA)和脂多糖(LPS)诱导的小鼠淋巴细胞增殖,提高免疫兔抗体水平和延长抗体持续时间;板蓝根多糖能增强ConA和LPS的诱导活性,蜂胶多糖能促进ConA的诱导活性,黄芪皂甙和淫羊藿黄酮能促进LPS诱导的淋巴细胞增殖,但它们都不能提高兔瘟组织灭活疫苗的免疫抗体水平。 相似文献
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本文报告了兔病毒性出血症(又称兔瘟)组织灭活苗的研制程序与测试结果。本疫苗是用人工感染兔的肝、脾、肾的10%组织匀浆中加入0.5%甲醛溶液37℃灭活24h而制成的。本疫苗安全性可靠,免疫原性良好。兔肌肉注射1mL,10d产生免疫力,近期保护率为100%。 相似文献
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Adjuvant effect of green propolis on humoral immune response of bovines immunized with bovine herpesvirus type 5 总被引:1,自引:0,他引:1
Fischer G Cleff MB Dummer LA Paulino N Paulino AS de Oliveira Vilela C Campos FS Storch T D'Avila Vargas G de Oliveira Hübner S Vidor T 《Veterinary immunology and immunopathology》2007,116(1-2):79-84
Despite recent technological advances in vaccine production, most vaccines depend on the association with adjuvant substances. In this study, propolis, which has been attracting the attention of researchers due to its bioactive properties, was evaluated as an immunological adjuvant. The association of 40mg/dose of an ethanolic extract of green propolis with an inactivated oil vaccine against bovine herpesvirus type 5 (BoHV-5), resulted in a significant increase (P<0.01) in the neutralizing antibody levels, comparing to the bovines that received the same vaccine without propolis. Besides, propolis increased the percentage of animals with high antibody titers (above 32). Phenolic compounds such as artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) and the derivatives of cinnamic acid besides other flavonoid substances were abundant in the propolis extract used, and they could be the main substances with adjuvant action. The effect of the green propolis extract on the humoral immune response can be exploited in the development of new vaccines. 相似文献
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E Aspoy 《Nordisk veterinaermedicin》1977,29(10):440-445
Propolis is a substance produced by honeybees. It is inhibitory to some bacteria species, mainly Gram-positive bacteria, but less inhibitory to Listeria monocytogenes (L.m) than to the other Gram-positive bacteria tested. In order to obtain selective growth of L.m. from contaminated samples, the effect of propolis in plating media and broths on various strains of bacteria was examined. Table I shows the effect of increasing concentrations of propolis in tryptose-agar (TA). L.m. tolerated higher concentrations of propolis than Streptococcus viridans and Staphylococcus aureus. L.m. grew well in tryptosebroth (TB) that contained 0.15 mg propolis pr. ml medium, while Streptococcus viridans and Streptococcus agalactiae were completely inhibited as seen in Table II. Table III shows that when serum was added to the agar, the inhibitory effect was reduced. It can also be seen that Gram-negative bacteria grew quite well on media that contained 0.19 mg propolis pr. ml. To reduce the growth of Gram-negative bacteria, nalidixic acid was added to the medium. Table IV illustrates growth of various species of bacteria in tryptosephosphatebroth (TFB) with or without propolis and nalidixic acid. Most of the strains tested were inhibited, but Pseudomonas aeruginosa and to some extent faecal streptococci were able to grow in the medium that contained the selective substances. As a conclusion it seems that propolis may be a valuable additive to a medium for the selective isolation of L.m. 相似文献
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为了探讨不同佐剂的奶牛乳房炎多联苗对小鼠免疫机能的影响,笔者将小白鼠分为3个组,每组30只,分别为蜂胶苗组、铝胶苗组和空白对照组。用制备的氢氧化铝胶和蜂胶2种乳房炎佐剂多联苗分别免疫小鼠,分别于免疫后的7、14、21、30和45 d采血,进行2种多联苗免疫后E-玫瑰花环率和淋巴细胞转化率测定。结果表明,蜂胶和铝胶2种佐剂苗与空白对照组相比均能显著提高小鼠的细胞免疫水平,2种苗的细胞免疫水平均在免疫后30 d左右达到最高峰(P<0.01),但在免疫后第30和45天时E-玫瑰花环率和淋巴细胞转化率,蜂胶苗组均极显著高于铝胶苗组(P<0.01),说明蜂胶佐剂比铝胶佐剂能更强地增强多联苗抗原对小鼠T细胞的致敏程度。该研究为进一步明确乳房炎多联苗免疫机制及筛选更好的免疫佐剂提供了科学数据。 相似文献
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用三个血清型的菌株,分别是JYL-1、JYL-2、JYL-7,用两种佐剂,分别是蜂胶和油乳剂共制备了两种多价菌苗,即JYL-1、JYL-2、JYL-7(1、2、7三个血清型)三个RA血清型混合多价蜂胶苗和油乳剂苗。试验结果表明。蜂胶复合佐剂多价苗具有产生免疫保护速度快,免疫持续时间长的优点,接种后第3天即产生部分免疫保护力,第120天时仍具有完全保护力;油乳剂多价苗产生保护力的速度较慢,接种后第10天时开始表现出部分免疫保护,其完全保护力也可持续到接种后第120天。免疫后13d时,免疫保护率可达90%左右。免疫后16d时保护率为100%。免疫后120d时,两种多价菌苗的免疫保护率均可达到100%,免疫后150~180d时,免疫保护率可达800左右。统计学分析证明,菌苗的安全性良好。用3个免疫剂量的菌苗所做的安全试验表明无明显不良反应,菌苗在4℃保存一年,18~22℃室温保存4个月不影响菌苗的免疫效力。田间试验表明,用菌苗免疫鸭群后,可有效地使鸭群抵抗鸭疫里默氏杆菌的感染。 相似文献
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作者制备了3批牦牛大肠埃希氏菌病蜂胶疫苗。制备了蜂胶佐剂和培养基,进行了牦牛大肠埃希氏菌的菌液培养、纯粹检验、活菌计数、灭活及无菌检验,配苗、分装及成品检验。3批牦牛大肠埃希氏菌病蜂胶疫苗无菌检验为阴性;物理性状检验为:静置后上层是乳黄色液体,下层为土黄色沉淀,振荡后呈均匀混浊液;经家兔安全检验结果为安全;经家兔效力试验,免疫后家兔抗体效价≥12.709lg2,对家兔具有免疫保护作用;蜂胶疫苗经防冻试验证明,随着蜂胶含量的增高,防冻效果越好。本试验为西藏牦牛大肠埃希氏菌病的预防提供依据。 相似文献
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