猪瘟免疫与仔猪腹泻防治的研究

一．陕西省流行的主要猪病 首先,我介绍一下我们学院所在的陕西省流行的主要猪病,初步调查统计,全省共发生猪病17种,分别是猪瘟、猪蓝耳病、猪圆环病毒病、副猪嗜血杆菌病、猪伪狂犬病、猪丹毒、猪肺疫、猪链球菌病、猪支原体肺炎、仔猪副伤寒、猪传染性萎缩性鼻炎、猪传染性胃肠炎、猪轮状病毒病、猪附红细胞体病、猪流行性腹泻、猪流感、猪大肠杆菌病等。     

猪瘟免疫与仔猪腹泻防治的研究

一、陕西省流行的主要猪病 首先,我介绍一下我们学院所在的陕西省流行的主要猪病,初步调查统计,全省共发生猪病17种,分别是猪瘟、猪蓝耳病、猪圆环病毒病、副猪嗜血杆菌病、猪伪狂犬病、猪丹毒、猪肺疫、猪链球菌病、猪支原体肺炎、仔猪副伤寒、猪传染性萎缩性鼻炎、猪传染性胃肠炎、猪轮状病毒病、猪附红细胞体病、猪流行性腹泻、猪流感、猪大肠杆菌病等.以猪瘟、蓝耳病、圆环病毒病、副猪嗜血杆菌病、伪狂犬病、流行性腹泻、传染性胃肠炎、口蹄疫等8种病为主,占所有猪病的88.7％.     

增强猪免疫力的七大途径

<正>目前,猪传染性接触性胸膜炎、副猪嗜血杆菌病、猪繁殖和呼吸综合征、猪圆环病毒II型感染、猪副红细胞体病、猪增生性肠炎、猪瘟、猪气喘病、5号病、仔猪大肠杆菌病、猪伪狂犬病、猪传染性萎缩性性鼻炎、猪链球菌病、猪布鲁氏菌病、猪流行性乙型脑炎病等病已是我国较大范围内猪场的常发病和多发病,并且多病原的多重感染或混合感染已是普遍发病的规     

2005年我国猪病流行现状及特点

1 猪传染病的种类增多,新病不断出现．表现为不同的发病原因及传播途径 2005年除猪瘟等老病外,包括猪链球菌病、猪传染性接触性胸膜炎、副猪嗜血杆菌病、猪繁殖和呼吸综合征、猪圆环病2型感染、猪附红细胞体病和猪增生性肠炎等新病涌现。加上原有在我国较多猪场发生的猪病、猪气喘病,5号病。仔猪大肠杆菌病。猪伪狂犬病、猪传染性萎缩性鼻炎、猪链球菌病、猪布鲁氏菌病、猪流行性乙型脑炎等,这些新旧传染病已是我国较大范围内猪场的常发病和多发病,真可谓旧病未除．新病又增．防不胜防,给养猪业造成极大的危害。     

猪腹泻病的诊治

以腹泻为主的猪病严重制约了猪的健康发展,给养猪业带来不少麻烦,而以腹泻为主的猪病的病因又比较复杂,主要有传染病细菌性猪腹泻大肠杆菌病(仔猪白痢、黄痢)、型魏氏梭菌病(仔猪红痢、梭菌型肠炎)、猪密螺旋体病(猪痢疾);病毒性猪腹泻,猪传染性胃肠炎、猪流行性腹泻、寄生虫病、中毒病等,因此猪出现腹泻     

猪呼吸道综合症及其防控

猪呼吸道病综合症是一种由多因素引起的呼吸道疾病的总称。近年来,在规模化猪场流行较广,造成猪只饲料转化率低、生长缓慢、治疗费用高、成活率低等,给养猪业带来重大损失,必须注意防控。 1发病原因 猪呼吸道病综合征是由病毒、细菌、环境应激和猪体免疫低下相互作用造成的,其中危害最大的是蓝耳病病毒、猪肺炎霉形体。此外,与猪呼吸道病综合征相关的主要疾病有：猪圆环病毒、猪瘟、猪伪犬病、猪断奶后多系统衰竭综合征、猪流感、猪气喘病、猪传染性胸膜肺炎、副猪嗜血杆菌病、猪传染性萎缩性鼻炎、猪链球菌病、猪肺炎、弓形虫病、猪附红细胞体病等。     

猪病的分类及常见病防治

猪病是影响养猪效益的重要因素,为了减少和避免猪病的影响,养殖人员必须加强专业知识学习,对猪病的分类特点及防治方法有正确的了解。本文对猪病的分类、猪病发生原因以及常见猪病的防治措施进行了介绍。     

恒丰强猪病诊治平台

夏末秋初猪条件性呼吸道疾病的发病特点及防控措施夏末秋初,是一年中猪病集中高发的季节。因天气潮热、霉菌泛滥、蚊虫滋生、应激频繁,导致猪只抵抗力降低,多数猪群处于一种亚健康状态。猪条件性疾病如副猪嗜血杆菌病、猪巴氏杆菌病、猪链球菌病、猪支原体肺炎、猪附红细胞体病等常以继发或     

猪的几种常见传染病宰后检疫中的区别

1.1 相同点：猪瘟和猪副伤寒均有纤维素性坏死性肠炎变化。1．2 不同点：猪副伤寒病猪脾肿大,肝有坏死和增生灶,肠系膜淋巴结髓样肿胀;猪瘟的肠黏膜通常有同心圆形的溃疡。     

当前一种危害严重的猪病——猪呼吸道病综合征(PRDC)

1 PRD C 的 病 因及 相互 关 系 1.1传染 性病 因 猪 呼吸 道 病综 合 征(PR DC)主要由 病毒 性 、细菌 性 、寄 生虫 三种 病 原引 起。 1.1.1病 毒性 病 原 。 主要 有 蓝 耳 病 病 毒 、猪 伪 狂犬病 病毒 、猪 流感 病 毒、猪Ⅱ型圆 环 病毒 、猪 瘟病 毒 、呼吸 道冠 状 病毒 等。 猪流 感 病毒 是 PR DC 的原 发 致病 病原 , 并 常混合 感 染 或 继 发 猪 传 染 性 胸 膜 肺 炎 、猪 肺 疫 、蓝 耳 病 、猪链 球菌 病 等,并 可 使猪 喘气 病 急性 暴发 。 伪 狂犬 病 病 毒 、猪 瘟 病毒 、衣 原 体 等 都 是 PR DC的常 见致 …     

Serological Investigations of Leptospiral Deoxyribonucleases

Enzymoserological comparison of a selection of leptospira strains tested with sera from rabbits immunized with unpurified DNase of Leptospira interrogans, serotype canicola, indicates the production of DNase of serologically very similar properties by the serotypes canicola, autumnalis, icterohemorrhagiae and pomona. The DNase produced by serotype hyos was serologically different from the others, while the serotypes grippotyphosa and bataviae did not produce DNases at all. The method used made it possible to differentiate between leptospiral DNase and normally occurring DNases in the serum samples. Neither leptospira DNase nor specific leptospira-DNase-antibodies could be detected in dog sera with high agglutinationlysis titres after natural infection.     

Immunity to leptospirosis: antiserums in dogs and hamsters.

Resistance to clinical and renal leptospirosis in dogs injected with antiserum to Leptospira interrogans serotype canicola and Leptospira interrogans serotype icterohaemorrhagiae was demonstrated. The relationship between resistance induced in serum-injected hamsters was also demonstrated, using criteria of leptospiremia, renal infection, leptospiruria, and clinical signs of disease in dogs and death in hamsters.     

Immunity to leptospirosis: bacterins in dogs and hamsters.

Resistance to clinical and renal leptospirosis in dogs vaccinated with a bacterin containing Leptospira interrogans serotype canicola and Leptospira interrogans serotype icterohaemorrhagiae was demonstrated. The relationship between resistance induced in vaccinated dogs and that induced in vaccinated hamsters was also demonstrated, using criteria of leptospiremia, renal infection, leptospiruria, and clinical signs of disease in dogs and death in hamsters.     

Rapid MALDI-TOF mass spectrometry identification of Leptospira organisms

Leptospirosis is a worldwide deadly zoonotic disease. Accurate identification of the causative Leptospira spp. spirochetes ascertains the pathogenic status of the isolates, identifies potential source of infection and recognises outbreaks. Species identification is currently based on technically demanding, time and resources consuming serological and molecular methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) recently emerged as a first-line method for the accurate identification of bacteria, yet no data issued for Leptospira spp. We investigated the potential of MALDI-TOF-MS for the rapid identification of Leptospira isolates. Starting from a 10(5)organisms/mL suspension, MALDI-TOF-MS yielded an unique protein profile for each one of 19 Leptospira species reference isolates with a 100% reproducibility over 12 repeats, allowing to create a Leptopsira database. MALDI-TOF-MS further accurately identified 20/21 additional reference isolates representative of various serogroups at the species level as Leptospira interrogans (n=12), Leptospira kirschneri (n=5), Leptospira borgpetersenii (n=3), Leptospira noguchii (n=1) with identification score value of 2-2.5. Furthermore, six clinical isolates previously identified by rpoB sequencing, were correctly identified by MALDI-TOF-MS as L. interrogans (n=5) and L. borgpetersenii (n=1) with identification score value of 2-2.6. Identification was achieved in 40 min starting from the Leptospira suspension. MALDI-TOF-MS could complement serological and sequencing-based methods for the first line, rapid identification of Leptospira isolates in the clinical microbiology laboratory.     

Serological survey for evidence of Leptospira interrogans in free-living platypuses (Ornithorhynchus anatinus)

Objective   To survey free-living platypuses for evidence of Leptospira interrogans .
Methods   A serological study of Leptospira antibodies was carried out on 21 platypuses captured between May and October 2001 in the Wollondilly River 200 km south of Sydney, New South Wales.
Results   Positive reactions, all to the L Hardjo serovar, were seen in 14 (66%) of the captured animals, with adult males showing a higher prevalence of antibodies than adult females. Several individual platypuses showed a high titre of L Hardjo antibodies, and some animals demonstrated cross-reactions to the serovars L Medanensis and L Kremastos.
Conclusion   The serological findings demonstrate that these animals are constantly exposed to infection with Leptospira in their environment, but it is not known if platypuses suffer from clinical leptospirosis or if they mount an immune response, but are unaffected by the bacteria. The prevalence of Leptospira infection among the platypus population could not be precisely estimated because of the unknown number of individuals inhabiting the Wollondilly River inside the survey property. Domestic livestock, mostly cattle, may be the major source of Leptospira infection. The effects of this disease on population dynamics and on reproduction in wild platypuses are not well understood. The role of other wildlife in the transmission and maintenance of Leptospira in the environment is unknown.     

Clinical Leptospira interrogans serogroup Australis serovar lora infection in a stud farm in The Netherlands

A Leptospira interrogans serogroup australis serovar lora infection in a stud farm is reported. During three successive years (1984-1986) clinical leptospirosis with a severe often rapid, fatal course was seen in 12 foals. Clinical examination revealed severe respiratory distress, depression and pyrexia. Other symptoms were diarrhea (2), jaundice (1), and an unsteady gait (1). Morphological characteristics of the disease were massive pulmonary haemorrhage and haemorrhagic-thrombotic or extracapillary glomerulonephritis with tubulonephrosis and interstitial oedema. In most foals high or increasing MAT titres to serovar bratislava were found; from one foal Leptospira interrogans serovar lora was isolated. Serological examination of all 56 mares at the farm (August 1986) revealed antibodies to serovar bratislava in 64 per cent of the animals. These findings support the idea that Leptospira interrogans serovar bratislava and closely related strains (in this study serovar lora) may be adapted to and maintained by the horse population.     

猪细小病毒检测技术研究进展

近年来,随着猪细小病毒分子生物学的研究进一步深入,各种新型疫苗的成功研制,为猪细小病毒病的防制带来希望。但目前为止猪细小病毒的检测技术以传统方法为主,大量研究发现猪细小病毒常与其他病原微生物混合感染,为了根除本病,必须提高诊断和防疫水平。文章就该病诊断方法的研究进展做一综述。     

Prevention of renal infection and urinary shedding in dogs by a Leptospira vaccination

Prevention of urinary shedding of Leptospira interrogans spp. by chronically infected dogs remains a key objective of the vaccination in dogs against leptospirosis which is a zoonotic disease. An inactivated bivalent vaccine composed of Leptospira interrogans serovars icterohaemorrhagiae [L. icterohaemorrhagiae] and canicola [L. canicola] bacterins was tested for its ability to protect puppies against a challenge exposure with L. icterohaemorrhagiae. The vaccine was administered twice at a 3-week interval to six puppies aged from 8 to 9 weeks. Six other puppies were used as unvaccinated controls. All puppies were challenged 2 weeks after the second vaccine injection by intraperitoneal (IP) administration of L. icterohaemorrhagiae (day 0). Clinical signs, haematological and biochemical changes and evidence of Leptospira in blood, urine and kidney were monitored for 4 weeks after the challenge exposure (days 0-28). Puppies were euthanised on day 28 for post-mortem and histological examinations of liver and kidney. Control group presented clinical pictures of severe or subclinical infection. One dog developed severe clinical signs (hypothermia, depression, anorexia, abdominal pain, dehydration, icterus, weight loss) and died on post-infection day (PID) 7 due to an acute renal failure. Gross and microscopic lesions were in accordance with this clinical pattern. In the five remaining control dogs, the challenge exposure induced mainly a systemic infection including leptospiraemia, leptospiruria and renal carriage. The vaccinated group remained healthy throughout the study period. In conclusion, immunisation with a Leptospira vaccine was shown to protect dogs against symptomatology and leptospiraemia, urine shedding and renal infection.     

套式PCR在猪流行性腹泻病毒感染病例诊断中的应用

猪流行性腹泻是对养猪业危害较大的一种疾病,导致腹泻的原因多种多样,给临床诊断带来了困难。本研究将套式PCR技术成功应用于猪流行性腹泻临床病例的快速诊断中,旨在为猪流行性腹泻临床病例的快速鉴别诊断提供一种新的思路。     

An old disease with a new face: canine leptospirosis does not lose its relevance

The clinical features of the disease are presented based on retrospective analysis of the records of eleven dogs diagnosed with leptospirosis using clinical signs and results of the microagglutination test (MAT) between 1991 and 1996. Additionally, Leptospira titres were determined in 30 healthy dogs and 20 hospitalised dogs without clinical or laboratory evidence of leptospirosis. A positive titre for L. grippotyphosa, L. pomona, L. bratislava, L. australis, L. icterohaemorrhagiae and/or L. canicola was found in 16 normal dogs and only one hospitalised patient. Eight of these dogs had titres of > or = 1:800. Only one of them had been vaccinated shortly before sampling. These results suggest that many dogs from the surroundings of Bern, Switzerland have contact with various Leptospira interrogans serovars. In ten healthy dogs, the Leptospira titre was determined before and four weeks after vaccination with leptospiral antigen. Only two of the dogs showed a serologically measurable response to the antigen contained in the vaccine. In dogs MAT titers presumably do not reliably reflect the immune status against leptospiral infections.