Kinetics of nitrification under upland and flooded soils of varying texture

Abstract

Higher rates of nitrification often reported in fine than in coarse textured soils may not be a direct effect of soil texture because in most of the earlier studies, soil water content has been usually expressed as gravimetric, volumetric or soil's water‐holding capacity without consideration of differences in density/ porosity for soils of varying texture. The same water content in texturally different soils could provide very different conditions of soil aeration and associated nitrifying activity. Effects of soil texture on nitrification was studied by incubating three semiarid subtropical soils having sandy loam, loam, and silty clay textures at 35°C for 30 days using water‐filled pore space (WFPS) as the criterion of soil aeration. Upland or aerobic soil conditions, simulated by incubating soil at 60% WFPS, exhibited very fast nitrification of added fertilizer nitrogen (N) and most of the applied 100 mg of ammonium‐nitrogen (NH₄+‐N/kg soil) was nitrified within 10 days of incubation in all three soils irrespective of the differences in texture. Under flooded soil conditions (120% WFPS), nitrification was slow and only 84 to 92% of the applied NH₄+‐N was nitrified even after 30 days. Nitrification could be described by first‐order kinetics for both the upland and flooded moisture regimes, thus nitrification rate depended upon NH₄+ concentration. At similar gravimetric water contents, rates of nitrification differed greatly in soils of varying texture, but when varying water‐holding capacity and bulk density were accounted for using WFPS, all the soils behaved similarly at 60% WFPS. Under impeded aeration (flooded conditions), however, substantial differences were observed in nitrification in soils of varying texture, the largest in fine‐textured Chamror silty clay followed by Habowal loam and the smallest in Tolewal sandy loam soil. These results illustrate the utility of WFPS, compared with soil water content, and its reliability as an indicator of aeration dependent nitrification for soils of varying texture.     

Degradation of 14C-Zinc Ammonium Acetate in Soils as Influenced by Soil Types,Soil Sterilization,and Carriers

Zinc ammonium acetate (ZAA), typically applied to soils in anhydrous ammonia as a carrier, has been used to improve corn (Zea maysL.) productivity. This study aimed to determine the fate of ZAA in soils as influenced by soil type (sandy, silt, and clay loam), sterilization (sterile and non-sterile), and two carriers (H₂O and NH₄OH). A 16 d laboratory incubation experiment with ¹⁴C-ZAA showed that total recovery of carbon-14 (¹⁴C) from ¹⁴CO₂ trap and soil extraction by CaCl₂ ranged from 72% to 94% in the first 8 d for sterilized soils. However, < 17% ¹⁴C was found in non-sterilized soils. Most ¹⁴C recovered in sterilized soil was associated with soil extraction, and relatively little was found in the CO₂ traps. All sterilized soils provided similar ¹⁴C recoveries except the sandy loam. Slightly more ¹⁴C was extracted from the soil when NH₄OH was the ZAA carrier rather than water. Conversely, recovery of ¹⁴CO₂ continued to increase during the 16 d incubation, but started faster when water was the ZAA carrier. Microbial activity appeared to be instrumental in the assimilation and disappearance of ZAA.     

Impact of addition of different rates of rice-residue biochar on C and N dynamics in texturally diverse soils

Impacts of crop residue biochar on soil C and N dynamics have been found to be subtly inconsistent in diverse soils. In the present study, three soils differing in texture (loamy sand, sandy clay loam and clay) were amended with different rates (0%, 0.5%, 1%, 2% and 4%) of rice-residue biochar and incubated at 25°C for 60 days. Soil respiration was measured throughout the incubation period whereas, microbial biomass C (MBC), dissolved organic C (DOC), NH₄⁺-N and NO₃^–N were analysed after 2, 7, 14, 28 and 60 days of incubation. Carbon mineralization differed significantly between the soils with loamy sand evolving the greatest CO₂ followed by sandy clay loam and clay. Likewise, irrespective of the sampling period, MBC, DOC, NH₄⁺-N and NO₃^–N increased significantly with increasing rate of biochar addition, with consistently higher values in loamy sand than the other two soils. Furthermore, regardless of the biochar rates, NO₃^–-N concentration increased significantly with increasing period of incubation, but in contrast, NH₄⁺-N temporarily increased and thereafter, decreased until day 60 in all soils. It is concluded that C and N mineralization in the biochar amended soils varied with the texture and native organic C status of the soils.     

Soil carbon responses to past and future CO2 in three Texas prairie soils

Changes in soil carbon storage could affect and be affected by rising atmospheric CO₂. However, it is unlikely that soils will respond uniformly, as some soils are more sensitive to changes in the amount and chemistry of plant tissue inputs whereas others are less sensitive because of mineralogical, textural, or microbial processes. We studied soil carbon and microbial responses to a preindustrial-to-future CO₂ gradient (250–500 ppm) in a grassland ecosystem in the field. The ecosystem contains three soil types with clay fractions of 15%–55%: a sandy loam Alfisol, a silty clay Mollisol, and a black clay Vertisol. Soil and microbial responses to atmospheric CO₂ are plant-mediated; and aboveground plant productivity in this ecosystem increased linearly with CO₂ in the sandy loam and silty clay. Although total soil organic carbon (SOC) did not change with CO₂ treatment after four growing seasons, fast-cycling SOC pools increased with CO₂ in the two clay soils. Microbial biomass increased 18% and microbial activity increased 30% across the CO₂ gradient in the black clay (55% clay), but neither factor changed with CO₂ in the sandy loam (15% clay). Similarly, size fractionation of SOC showed that coarse POM-C, the youngest and most labile fraction, increased four-fold across the CO₂ gradient in the black clay, but increased by only 50% across the gradient in the sandy loam. Interestingly, mineral-associated C, the oldest and most recalcitrant fraction, declined 23% across the gradient in the third soil type, a silty clay (45% clay). Our results provide evidence for priming in this soil type, as labile C availability and decomposition rate (measured as soil respiration and soil C mineralization) also increased across the CO₂ gradient in the silty clay soil. In summary, CO₂ enrichment in this grassland increased the fast-cycling SOC pool as in other CO₂ studies, but only in the two high-clay soils. Priming in the silty clay could limit SOC accumulation after prolonged CO₂ exposure. Because soil texture varies geographically, including data on soil types could enhance predictions of soil carbon and microbial responses to future CO₂ levels.     

Greenhouse gas emissions after application of digestate: short-term effects of nitrification inhibitor and application technique effects

Nitrogen-use efficiency in arable agriculture after organic fertilization can be improved by the incorporation of digestate into soil and through the use of nitrification inhibitors. To test the efficiency and the interaction of these measures, a laboratory microcosm study was conducted with undisturbed samples from two arable soils – a Gleysol and a Plaggic Anthrosol. Treatments were digestate application by injection to 15 or 20 cm depths or by trailing hose with subsequent incorporation. Half of the replicates of each application treatment were treated with the nitrification inhibitor 3,4-dimethyl pyrazole phosphate (DMPP). Emissions of the greenhouse gases (GHGs) CO₂, N₂O and CH₄ were monitored during 51 days of incubation. Deeper injection (20 cm) did not lead to different GHG emissions compared with a shallow injection (15 cm). Application of DMPP decreased cumulative N₂O emissions significantly by 17–70%. DMPP inhibited N₂O fluxes and NO₃- production, suggesting a positive effect of DMPP on the mitigation of direct GHG emission and nitrate leaching at least during several weeks after digestate fertilization. The effect of DMPP is independent of the application technique.     

Influence of homogenized residues of anaerobic digestate on the physicochemical properties of differently textured soils

Because of the focus on renewable energy, new biogas digesters are being built with the consequence of an increased production of anaerobic digestates (AD) as a by‐product. Although they can be used as organic fertilizer on arable fields, negative impacts of these digestates also may occur. Therefore, it was the aim of this laboratory study to investigate the effect of a normally applied volume of 30 m³ ha^?1 of anaerobic digestates derived from a ground input substrate of maize (Zea mays L.) , sugar beet (Beta vulgaris L.), and wheat (Triticum aestivum L.) in different ratios (100 /80 / 20%) on the properties of two soils. The soils, which were homogenized (sieved to ≤ 2 mm) and placed in columns with a defined bulk density of 1.45 g cm^?3, were a Cambic Luvisol (sandy loam) derived from glacial till and a Podzol (sandy sand) derived from glazial outwash. Physicochemical parameters [pH, electrical conductivity (EC)] and the wetting behavior of the soils were analyzed by measuring the contact angle (CA) by using the Wilhelmy–Plate‐Method (WPM) and the Repellency Index (RI) from the sorptivity of water and ethanol. To determine the risk of soil dispersion as a consequence of digestate amendment, the amount of readily dispersible clay (RDC) was determined by detecting the turbidity of a soil suspension. The application of 30 m³ ha^?1 of AD decreased the wettability of the sandy sand as compared to the untreated soil, while the wettability of the loamy sand remained unaffected by the digestate amendment. The amount of RDC was higher in the loamy sand compared to the sandy sand, but the AD‐amended soil did not exhibit a significant change in dispersibility. While the loamy soil exhibited acidification of the soil after digestate application, the sandy soil showed an alkalinization of soil columns. Overall, the soil texture was identified to be a main factor controlling the effect of the digestates on soil properties. The results of this lab study showed that this study can be used as a first approach for the quantification of digestate amendment under practical conditions.     

Mineralization of carbon and nitrogen from fresh and anaerobically stored sheep manure in soils of different texture

A sandy loam soil was mixed with three different amounts of quartz sand and incubated with (¹⁵NH₄)₂SO₄ (60 g N g^-1 soil) and fresh or anaerobically stored sheep manure (60 g g^-1 soil). The mineralization-immobilization of N and the mineralization of C were studied during 84 days of incubation at 20°C. After 7 days, the amount of unlabelled inorganic N in the manure-treated soils was 6–10 g N g^-1 soil higher than in soils amended with only (¹⁵NH₄)₂SO₄. However, due to immobilization of labelled inorganic N, the resulting net mineralization of N from manure was insignificant or slightly negative in the three soil-sand mixtures (100% soil+0% quartz sand; 50% soil+50% quartz sand; 25% soil+75% quartz sand). After 84 days, the cumulative CO₂ evolution and the net mineralization of N from the fresh manure were highest in the soil-sand mixutre with the lowest clay content (4% clay); 28% fo the manure C and 18% of the manure N were net mineralized. There was no significant difference between the soil-sand mixtures containing 8% and 16% clay, in which 24% of the manure C and -1% to 4% of the manure N were net mineralized. The higher net mineralization of N in the soil-sand mixture with the lowest clay content was probably caused by a higher remineralization of immobilized N in this soil-sand mixture. Anaerobic storage of the manure reduced the CO₂ evolution rates from the manure C in the three soil-sand mixtures during the initial weeks of decomposition. However, there was no effect of storage on net mineralization of N at the end of the incubation period. Hence, there was no apparent relationship between net mineralization of manure N and C.     

Combined effects of nitrogen deposition and biochar application on emissions of N2O,CO2 and NH3 from agricultural and forest soils

Abstract

Both nitrogen (N) deposition and biochar can affect the emissions of nitrous oxide (N₂O), carbon dioxide (CO₂) and ammonia (NH₃) from different soils. Here, we have established a simulated wet N deposition experiment to investigate the effects of N deposition and biochar addition on N₂O and CO₂ emissions and NH₃ volatilization from agricultural and forest soils. Repacked soil columns were subjected to six N deposition events over a 1-year period. N was applied at rates of 0 (N0), 60 (N60), and 120 (N120) kg Nh a^?1 yr^?1 without or with biochar (0 and 30 t ha^?1 yr^?1). For agricultural soil, adding N increased cumulative N₂O emissions by 29.8% and 99.1% (p < 0.05) from the N60 and N120 treatments, respectively as compared to without N treatments, and N120 emitted 53.4% more (p < 0.05) N₂O than the N60 treatment; NH₃ volatilization increased by 33.6% and 91.9% (p < 0.05) from the N60 and N120 treatments, respectively, as compared to without N treatments, and N120 emitted 43.6% more (p < 0.05) NH₃ than N60; cumulative CO₂ emissions were not influenced by N addition. For forest soil, adding N significantly increased cumulative N₂O emissions by 141.2% (p < 0.05) and 323.0% (p < 0.05) from N60 and N120 treatments, respectively, as compared to without N treatments, and N120 emitted 75.4% more (p < 0.05) N₂O than N60; NH₃ volatilization increased by 39.0% (p < 0.05) and 56.1% (p < 0.05) from the N60 and N120 treatments, respectively, as compared to without N treatments, and there was no obvious difference between N120 and N60 treatments; cumulative CO₂ emissions were not influenced by N addition. Biochar amendment significantly (p < 0.05) decreased cumulative N₂O emissions by 20.2% and 25.5% from agricultural and forest soils, respectively, and increased CO₂ emissions slightly by 7.2% and NH₃ volatilization obviously by 21.0% in the agricultural soil, while significantly decreasing CO₂ emissions by 31.5% and NH₃ volatilization by 22.5% in the forest soil. These results suggest that N deposition would strengthen N₂O and NH₃ emissions and have no effect on CO₂ emissions in both soils, and treatments receiving the higher N rate at N120 emitted obviously more N₂O and NH₃ than the lower rate at N60. Under the simulated N deposition circumstances, biochar incorporation suppressed N₂O emissions in both soils, and produced contrasting effects on CO₂ and NH₃ emissions, being enhanced in the agricultural soil while suppressed in the forest soil.     

Soil N Losses by Denitrification Evaluated Using the 15N Tracer Method

Molecular nitrogen (N₂) and nitrous oxide (N₂O) generated by denitrification increase N losses in the soil–plant system. This study aimed to quantify N₂ and N₂O from potassium nitrate (K¹⁵NO₃) applied to soils with different textures and moisture contents in the absence and presence of a source of carbon (C) using the ¹⁵N tracer method. In the three soils used (sandy texture (ST), sandy clay loam texture (SCLT), and clayey texture (CT)), three moisture contents were evaluated (40%, 60%, and 80% of the water holding capacity (WHC)) with (D⁺) and without (D^?) dextrose added. The treatments received 100 mg N kg^?1 (KNO₃ with 23.24 atom% ¹⁵N). N₂ emissions occurred in all of the treatments, but N₂O emissions only occurred in the D⁺ treatment, showing increases with increasing moisture content. SCLT with 80% WHC in the D⁺ treatment exhibited the highest accumulated N emission (48.26 mg kg^?1). The ¹⁵N balance suggested trapping of the gases in the soil.     

Turnover of root-derived material and related microbial biomass formation in soils of different texture

Wheat plants were grown on two soils of different texture, a sandy soil and a silty clay loam, in an atmosphere containing ¹⁴CO₂. The ¹⁴C and total C content of the shoots, roots, soil rhizosphere CO₂ and soil microbial biomass were measured 21, 28, 35 and 42 days after germination. There was a pronounced effect of soil texture on the turnover of root-derived C through the microbial biomass. Turnover was relatively fast and at a constant rate in the sandy soil but slowed down in the clay soil, following an initial high assimilation of root products into the microbial biomass.Four percent of the total fixed ¹⁴C was retained in the clay loam after 6 weeks compared with a corresponding value of 1.2% for the sandy soil. The proportion of fixed ¹⁴C recovered as rhizosphere CO₂ at each of the sampling times was relatively constant for the sandy soil (ca 19%) but decreased from 17% at day 28 to 11% at day 42 in the clay soil. The proportion of total fixed ¹⁴C in the soil biomass as measured by a fumigation technique increased to a maximum value of 20% after 6 weeks in the sandy soil but decreased in the clay soil from 86% at day 21 to 26% after 42 days plant growth.     

Effect of elevated CO2 on soil N dynamics in a temperate grassland soil

The response of terrestrial ecosystems to elevated atmospheric CO₂ is related to the availability of other nutrients and in particular to nitrogen (N). Here we present results on soil N transformation dynamics from a N-limited temperate grassland that had been under Free Air CO₂ Enrichment (FACE) for six years. A ¹⁵N labelling laboratory study (i.e. in absence of plant N uptake) was carried out to identify the effect of elevated CO₂ on gross soil N transformations. The simultaneous gross N transformation rates in the soil were analyzed with a ¹⁵N tracing model which considered mineralization of two soil organic matter (SOM) pools, included nitrification from NH₄⁺ and from organic-N to NO₃⁻ and analysed the rate of dissimilatory NO₃⁻ reduction to NH₄⁺ (DNRA). Results indicate that the mineralization of labile organic-N became more important under elevated CO₂. At the same time the gross rate of NH₄⁺ immobilization increased by 20%, while NH₄⁺ oxidation to NO₃⁻ was reduced by 25% under elevated CO₂. The NO₃⁻ dynamics under elevated CO₂ were characterized by a 52% increase in NO₃⁻ immobilization and a 141% increase in the DNRA rate, while NO₃⁻ production via heterotrophic nitrification was reduced to almost zero. The increased turnover of the NH₄⁺ pool, combined with the increased DNRA rate provided an indication that the available N in the grassland soil may gradually shift towards NH₄⁺ under elevated CO₂. The advantage of such a shift is that NH₄⁺ is less prone to N losses, which may increase the N retention and N use efficiency in the grassland ecosystem under elevated CO₂.     

C and N turnover in structurally intact soils of different texture

The turnover of native and applied C and N in undisturbed soil samples of different texture but similar mineralogical composition, origin and cropping history was evaluated at −10 kPa water potential. Cores of structurally intact soil with 108, 224 and 337 g clay kg⁻¹ were horizontially sliced and ¹⁵N-labelled sheep faeces was placed between the two halves of the intact core. The cores together with unamended treatments were incubated in the dark at 20 °C and the evolution of CO₂-C determined continuously for 177 d. Inorganic and microbial biomass N and ¹⁵N were determined periodically. Net nitrification was less in soil amended with faeces compared with unamended soil. When adjusted for the NO₃-N present in soil before faeces was applied, net nitrification became negative indicating that NO₃-N had been immobilized or denitrified. The soil most rich in clay nitrified least N and ¹⁵N. The amounts of N retained in the microbial biomass in unamended soils increased with clay content. A maximum of 13% of the faeces ¹⁵N was recovered in the microbial biomass in the amended soils. CO₂-C evolution increased with clay content in amended and unamended soils. CO₂-C evolution from the most sandy soil was reduced due to a low content of potentially mineralizable native soil C whereas the rate constant of C mineralization rate peaked in this soil. When the pool of potentially mineralizable native soil C was assumed proportional to volumetric water content, the three soils contained similar proportions of potentially mineralizable native soil C but the rate constant of C mineralization remained highest in the soil with least clay. Thus although a similar availability of water in the three soils was ensured by their identical matric potential, the actual volume of water seemed to determine the proportion of total C that was potentially mineralizable. The proportion of mineralizable C in the faeces was similar in the three soils (70% of total C), again with a higher rate constant of C mineralization in the soil with least clay. It is hypothesized that the pool of potentially mineralizable C and C rate constants fluctuate with the soil water content.     

Extractability of 15N-labeled corn-shoot tissue in a sandy and a clay soil by 0.01 MCaCl2 method in laboratory incubation experiments

Management of N fertilization depends not only on the mineral N measured at the beginning of the growing season but also on the status of the low-molecular-weight organic-N fraction. Our study was conducted to analyze how much of the ¹⁵N applied in labeled cornshoot tissue would be recovered in 0.01 M CaCl₂-extractable ¹⁵N fractions and wheter a decrease in the CaCl₂-extractable ¹⁵N fraction quantitatively followed the trend in net mineralization of the ¹⁵N applied in corn-shoot tissue during an incubation period. The effects of adding ¹⁵N-labeled young corn-shoot tissue to a sandy soil and a clay soil were investigated for 46 days in an aerobic incubation experiment at 25°C. The application of 80 mg N kg^-1 soil in the form of labeled corn-shoot tissue (24.62 mg ¹⁵N kg^-1 soil) resulted in a significant initial increase, followed by a decrease the labeled organic-N fraction in comparison with the untreated soils during the incubation. The labeled organic-N fraction was significantly higher in the sandy soil than in the clay soil until the 4th day of incubation. The decrease in labeled organic N in the sandy soil resulted in a subsequent increase in ¹⁵NO _inf3 ^sup- during the incubation. Ammonification of applied plant N resulted in a significant increase in the 1 M HCl-extractable non-exchangeable ¹⁵NH _inf4 ^sup+ fraction in the clay soik, owing to the vermiculite content. The ¹⁵N recovery was analyzed by the 0.01 M CaCl₂ extraction method; at the beginning of the incubation experiment, recovery was 37.0% in the sandy soil and 36.7% in the clay soil. After 46 days of incubation, recovery increased to 47.2 and 43.8% in the sandy and clay soils, respectively. Net mineralization of the ¹⁵N applied in corn-shoot tissue determined after the 46-day incubation was 6.60 mg ¹⁵N kg^-1 soil (=34.9% of the applied organic ¹⁵N) and 4.37 mg ¹⁵N kg^-1 soil (=23.1% of the applied organic ¹⁵N) in the sandy and the clay soils, respectively. The decrease in the labeled organic-N fraction extracted by 0.01 M CaCl₂ over the whole incubation period was 3.14 and 2.33 mg ¹⁵N kg^-1 soil in the sandy and clay soil, respectively. These results indicate that net mineralization of ¹⁵N was not consistent with the decrease in the labeled organic-N fraction. This may have been due to the inability of 0.01 M CaCl₂ to extract or desorb all of the applied organic ¹⁵N that was mineralized during the incubation period.     

Nitrogen transformation in arable soils of North-West Germany during the cereal growing season

In 1991, field experiments on loess (with winter wheat) and sandy soils (with summer barley) were conducted to study N dynamics in the microbial biomass and non-exchangeable NH _inf4 ^sup+ . The measurements showed a mass change in microbial N, with a maximum increase of 100 kg N ha^-1 30 cm^-1 from March to July in the loess soil, and a change for only 1 month (May) in the sandy soil. Plots treated with conventional levels of N fertilizer (213 kg N ha^-1 on a loess soil to winter wheat and 130 kg ha^-1 on the sandy soil to summer barley), reduced levels of N (83% and 62% of the conventional N application), or no N showed no consistent fertilizer N effect on microbial biomass N. From March to July, non-exchangeable NH _inf4 ^sup+ in loess soils under winter wheat decreased by 110 kg N ha^-1 30 cm^-1 in conventionally fertilized plots and by 200 kg N ha^-1 30 cm^-1 in a plot with no N fertilizer. After harvest, the pool of non-exchangeable NH _inf4 ^sup+ increased due to increasing mineral N concentrations in the soil.     

Effects of ammonium and nitrate additions on carbon mineralization in wetland soils

Wetlands have been recognized as a soil carbon (C) sink due to low decomposition. As decomposition is largely controlled by the availability of soil nitrogen (N), an elevated anthropogenic N input could influence the C balance in wetlands. However, the effects of the form of N on decomposition are poorly understood. Here, a 54-day laboratory incubation experiment was conducted, with a diel cycle (day: 22 °C for 13 h; night: 17 °C for 11 h) in order to determine how the dominant N form influences the mineralization of soil C in two adjacent wetland soils, with distinct physicochemical characteristics. Three combinations of N compounds were added at three different rates (0, 30, 60 kg N ha⁻¹ yr⁻¹): Ammonium dominant (NH₄Cl + NH₄NO₃); nitrate dominant (NH₄NO₃ + NaNO₃); and ammonium nitrate treatments (NH₄NO₃). In the acidic soil, the CO₂ efflux was reduced with N additions, especially with NH₄NO₃ treatment. In addition, decreases in the microbial enzyme activities (β-glucosidase, N-acetyl-glucosaminidase, phosphatase, and phenol oxidase) and soil pH were observed with NH₄NO₃ and -dominant treatment. Under alkaline conditions, marginal changes in response to N additions were observed in the soil CO₂ efflux, extractable DOC, simple substrate utilization, enzyme activities and pH. A regression analysis revealed that the changes in pH and enzyme activities after fertilization significantly influenced the soil CO₂ efflux. Our findings suggest that the form of N additions could influence the rate of C cycling in wetland soils via biological (enzyme activities) and chemical (pH) changes.     

土壤有机碳对沼渣或牛粪施用后温室气体排放潜力的影响—盆栽试验结果

A change in the European Union energy policy has markedly promoted the expansion of biogas production.Consequently,large amounts of nutrient-rich residues are being used as organic fertilizers.In this study,a pot experiment was conducted to simulate the high-risk situation of enhanced greenhouse gas (GHG) emissions following organic fertilizer application in energy maize cultivation.We hypothesized that cattle slurry application enhanced CO2 and N2O fluxes compared to biogas digestate because of the overall higher carbon (C) and nitrogen (N) input,and that higher levels of CO2 and N2O emissions could be expected by increasing soil organic C (SOC) and N contents.Biogas digestate and cattle slurry,at a rate of 150 kg NH4+-N ha-1,were incorporated into 3 soil types with low,medium,and high SOC contents (Cambisol,Mollic Gleysol,and Sapric Histosol,termed Clow,Cmedium,and Chigh,respectively).The GHG exchange (CO2,CH4,and N2O) was measured on 5 replicates over a period of 22 d using the closed chamber technique.The application of cattle slurry resulted in significantly higher CO2 and N2O fluxes compared to the application of biogas digestate.No differences were observed in CH4 exchange,which was close to zero for all treatments.Significantly higher CO2 emissions were observed in Chigh compared to the other two soil types,whereas the highest N2O emissions were observed in Cmedium.Thus,the results demonstrate the importance of soil type-adapted fertilization with respect to changing soil physical and environmental conditions.     

Organic carbon mineralization responses to temperature increases in subtropical paddy soils

Purpose

Understanding organic carbon mineralization and its temperature response in subtropical paddy soils is important for the regional carbon balance. There is a growing interest in factors controlling soil organic carbon (SOC) mineralization because of the potential for climate change. This study aims to test the hypothesis that soil clay content impedes SOC mineralization in subtropical paddy soils.

Materials and methods

A 160-day laboratory incubation at temperatures from 10 to 30 °C and 90% water content was conducted to examine the dynamics of SOC mineralization and its temperature response in three subtropical paddy soils with different clay contents (sandy loam, clay loam, and silty clay soils). A three-pool SOC model (active, slow, and resistant) was used to fit SOC mineralization.

Results and discussion

Total CO₂ evolved during incubation following the order of clay loam > silty clay > sandy loam. The temperature response coefficients (Q ₁₀) were 1.92?±?0.39, 2.36?±?0.22, and 2.10?±?0.70, respectively, for the sandy loam soil, clay loam soil, and silty clay soil. But the soil clay content followed the order of silty clay > clay loam > sandy loam. The sandy loam soil neither released larger amounts of CO₂ nor showed higher temperature sensitivity, as expected, even though it contains lower soil clay content among the three soils. It seems that soil clay content did not have a dominant effect which results in the difference in SOC mineralization and its temperature response in the selected three paddy soils. However, dissolved organic carbon (DOC; representing substrate availability) had a great effect. The size of the active C pool ranged from 0.11 to 3.55% of initial SOC, and it increased with increasing temperature. The silty clay soil had the smallest active C pool (1.40%) and the largest Q ₁₀ value (6.33) in the active C pool as compared with the other two soils. The mineralizable SOC protected in the silty clay soil, therefore, had even greater temperature sensitivity than the other two soils that had less SOC stabilization.

Conclusions

Our study suggests that SOC mineralization and its temperature response in subtropical paddy soils were probably not dominantly controlled by soil clay content, but the substrate availability (represented as DOC) and the specific stabilization mechanisms of SOC may have great effects.     

Changes in forest soil organic matter pools after a decade of elevated CO2 and O3

The impact of rising atmospheric carbon dioxide (CO₂) may be mitigated, in part, by enhanced rates of net primary production and greater C storage in plant biomass and soil organic matter (SOM). However, C sequestration in forest soils may be offset by other environmental changes such as increasing tropospheric ozone (O₃) or vary based on species-specific growth responses to elevated CO₂. To understand how projected increases in atmospheric CO₂ and O₃ alter SOM formation, we used physical fractionation to characterize soil C and N at the Rhinelander Free Air CO₂-O₃ Enrichment (FACE) experiment. Tracer amounts of ¹⁵NH₄⁺ were applied to the forest floor of Populus tremuloides, P. tremuloides-Betula papyrifera and P. tremuloides-Acer saccharum communities exposed to factorial CO₂ and O₃ treatments. The ¹⁵N tracer and strongly depleted ¹³C-CO₂ were traced into SOM fractions over four years. Over time, C and N increased in coarse particulate organic matter (cPOM) and decreased in mineral-associated organic matter (MAOM) under elevated CO₂ relative to ambient CO₂. As main effects, neither CO₂ nor O₃ significantly altered ¹⁵N recovery in SOM. Elevated CO₂ significantly increased new C in all SOM fractions, and significantly decreased old C in fine POM (fPOM) and MAOM over the duration of our study. Overall, our observations indicate that elevated CO₂ has altered SOM cycling at this site to favor C and N accumulation in less stable pools, with more rapid turnover. Elevated O₃ had the opposite effect, significantly reducing cPOM N by 15% and significantly increasing the C:N ratio by 7%. Our results demonstrate that CO₂ can enhance SOM turnover, potentially limiting long-term C sequestration in terrestrial ecosystems; plant community composition is an important determinant of the magnitude of this response.     

Chemical fixation of ammonia to soil organic matter after application of urea

Chemical fixation of NH₃ to soil organic matter was studied in two Swedish soils with different contents of organic matter: a clay soil with 2.3% C and an organic soil with 36.6% C. ¹⁵N‐labelled urea was applied at different rates to both sterilized and non‐sterilized soils. After 10 days, the soils were extracted and washed with K₂SO₄ and determined for total N and atom% ¹⁵N excess. Urea N was recovered as non‐extractable N in sterilized soil corresponding to 9.7% of supplied ^l5N‐labelled urea in the organic soil and 2.2% in the clay soil. Since no biological immobilization is thought to occur in the sterile soil, this non‐extractable N is suggested to be chemically fixed to soil organic matter. Owing to urea hydrolysis in the clay soil, pH increased from 6.3 to 9.3 and in the organic soil from 5.7 to 6.9 and 8.8, respectively, at the low and high urea supply.     

NH3 Volatilization,N2O Emission and Microbial Biomass Turnover from 15N-Labeled Manure Under Laboratory Conditions

On irrigated agricultural soils from semi-arid and arid regions, ammonia (NH₃) volatilization and nitrous oxide (N₂O) emission can be a considerable source of N losses. This study was designed to test the capture of ¹⁵N loss as NH₃ and N₂O from previous and recent manure application using a sandy, calcareous soil from Oman amended one or two times with ¹⁵N labeled manure to elucidate microbial turnover processes under laboratory conditions. The system allowed to detect ¹⁵N enrichments in evolved N₂O-N and NH₃-N of up to 17% and 9%, respectively, and total N, K₂SO₄ extractable N and microbial N pools from previous and recent ¹⁵N labeled manure applications of up to 7%, 8%, and 15%. One time manured soil had higher cumulative N₂O-N emissions (141 µg kg^?1) than repeatedly manured soil with 43 µg kg^?1 of which only 22% derived from recent manure application indicating a priming effect.