Handling sunflower (Helianthus annuus L.) populations under heat stress

Sustainable sunflower yield is under threat by a significant rise in global temperatures. Therefore, the development of a heat-resistant sunflower breeding population or hybrid is important for sustainable yield under high temperatures. Although there are a significant number of studies aimed at understanding the mechanism of heat resistance, only few direct approaches were made to improve heat resistance in sunflower. When breeding for heat resistance, the selection criteria are important. A desirable screening criterion should be rapid, highly heritable, and allow for the discrimination of resistant and susceptible ecotypes before reproductive maturity so that undesirable plants can be eliminated. In contrast, destructive selection criteria are not applicable for a segregating population. On the basis of an extensive review of data and experimentation, traits such as leaf and head orientation at the time of anthesis are proposed for practical utilization of sunflower heat-resistant breeding. These traits could be utilized as markers linked with heat avoidance. The traits are simply inherited, non-destructive, non-laborious, and show no impact of the environment and are independent of genotype × environment.     

NPK could alleviate the adverse effects of simulated acid rain in sunflower (Helianthus annuus L.)

A study was conducted to determine the effect of macronutrients (NPK) in alleviating the adverse effects of simulated acid rain (SAR) on sunflower (Helianthus annuus L.). In addition to control (T₀), three different treatments, i.e., SAR (HNO₃) of pH 3 (T₁), NPK (T₂), and SAR + NPK (T₃), were applied on two sunflower cultivars, i.e., FH-37 and FH-385. The experiment was set up in CRD (completely randomized design) with four replicates of each treatment. Chlorophyll a, b, total chlorophyll, carotenoids, ion contents (NPK), and gas exchange characters were determined. Acid rain remarkably reduced the chlorophyll pigments, NPK ionic content, photosynthetic rate, transpiration rate, and stomatal conductance, while an increase in internal CO₂ concentration and water use efficiency was noted in both the cultivars. The mixture of NPK with SAR exhibited positive impact to lessen the toxicity caused by acid. Among cultivars, FH-385 showed better performance as compared to FH-37.     

Differential allelopathic potential of sunflower (Helianthus annuus L.) genotypes on weeds and wheat (Triticum aestivum L.) crop

Studies were conducted to screen eight sunflower (Helianthus annuus L.) genotypes for their allelopathic potential against weeds and wheat crop, which customarily follows sunflower in Iraq. All sunflower genotypes significantly inhibited the total number and biomass of companion weeds and the magnitude of inhibition was genotype dependent. Among the eight genotypes tested, Sin-Altheeb and Coupon were the most weed-suppressing cultivars, and Euroflor and Shumoos were the least. A subsequent field experiment indicated that sunflower residues incorporated into the field soil significantly inhibited the total number and biomass of weeds growing in the wheat field. Sunflower genotypes Sin-Altheeb and Coupon appeared to inhibit total weed number and biomass more and significantly increased wheat yield compared with the least-suppressive genotypes (Euroflor and Shumoos). Chromatographic analyses by HPLC revealed the presence of 13 secondary metabolites in residues of the tested sunflower genotypes. All the isolated compounds appeared to be phenolic, with the exception of terpinol, which is a terpenoid derivative. The total concentration of Phytotoxins (phenolic compounds) was found to be higher in the most-suppressive potential genotypes compared with the least-suppressive genotypes.     

Growth kinetics at early stages of sunflower (Helianthus annuus L.) under soil compaction

Sunflower rooting depth is strongly related with soil structural behavior and gravimetric water availability. A few studies have been done on sunflower, only one within a kinetic aspect, and none involving fine root study. This work's aim was to study the impact of soil compaction and its interactions with soil water content on sunflower root and shoot growth and growth rate. A destructive experiment in controlled conditions was implemented to determine the consequences of soil compaction in interaction with water management on the growth of sunflower root and shoot system. Strong modifications on root exploration, architecture and growth were reported under low and high compactions depending on their water regime, the stage sampled and the time duration. This had a negative impact on resources uptake and efficiency. Modifications on the above ground part of the plant through plant water and nutrients uptake, plant growth indicators, biomass production and leaves growth kinetics were also observed.     

Boron-induced improvement in physiological,biochemical and growth attributes in sunflower (Helianthus annuus L.) exposed to terminal drought stress

Terminal drought stress (drought at reproductive growth stage) has been considered a severe environmental threat under changing climatic scenarios and undoubtedly inhibits sunflower production. A field study was conducted to explore the potential role of foliar applied boron (B) (0, 15, 30, 45 mg L^?1) at late growth periods of sunflower in alleviating the adversities of terminal drought stress (75, 64, 53 mm DI) grown from inflorescence emergence to maturity stages. The plant water relations such as leaf relative water content (RWC), water potential (Ψ_w), osmotic potential (Ψ_s), and turgor pressure (Ψ_p) were increased significantly with B foliar sprays while exposed to terminal drought stress. Foliar B application considerably improved the nitrogen and B concentrations in leaf and seed tissues, and also chlorophyll a and b pigments under terminal drought stress conditions. Drought-induced proline accumulation prevented the damages caused by drought stress, nevertheless, B foliar spray increased its contents. Compared to well-watered conditions, terminal drought stress substantially declined the growth performance in terms of reduced leaf area index (LAI), crop growth rate (CGR), net assimilation rate (NAR), and total dry matter (TDM) production; however, foliar B supply (30 mg L^?1) might be helpful for improving drought tolerance in sunflower with reduced growth losses.     

Potassium efficiency of safflower (Carthamus tinctorius L.) and sunflower (Helianthus annuus L.)

The oil crop safflower may have a certain production potential under low‐input conditions (organic farming, developing countries), where the putatively low nutrient requirement is highly welcomed. However, current knowledge regarding the nutrient use efficiency of safflower as compared to similar oil crops is limited. It was thus the aim of this study to determine the potassium (K) use efficiency of safflower (Carthamus tinctorius L.) as compared to sunflower (Helianthus annuus L.). Safflower and sunflower were cultivated with increasing K supply in a mixture of equal volumes of sand, nutrient‐poor limed soil, and perlite in 5 L Mitscherlich pots. Both species responded strongly to increasing K supply with respect to growth and yield. Safflower out‐yielded sunflower at low K supply, while at high K level, the opposite was observed. Both species accumulated similar amounts of K in shoots at low K supply. Only at extremely low K supply, safflower took up more K than sunflower. However, achene yield of sunflower exceeded that of safflower at optimal and high K supply. Safflower utilized absorbed K more efficiently than sunflower to produce achene yield at suboptimal K supply in terms of both efficiency ratio and utilization index. The efficiency of a crop to use supplied or accumulated K for dry‐matter and achene production was interpreted in terms of Michaelis‐Menten kinetics, specifically addressing the shape of the yield response curve. Indeed, the efficiency of safflower to use K for growth and yield, analogue to a low K_m in enzyme kinetics, was higher than in sunflower, while the K supply or K accumulation required to initiate yield formation in safflower was significantly lower. Similarly, safflower had a lower external K requirement for achene yield than sunflower at low and optimal K supplies. It can be concluded that safflower represents a low‐input crop and outperforms sunflower on soils low in available K. The data analysis also reveals that using just one efficiency indicator is usually not sufficient to adequately describe the K efficiency of the crop under consideration.     

The potential contribution of siderophore producing bacteria on growth and Fe ion concentration of sunflower (Helianthus annuus L.) under water stress

In the present study, 100 bacterial strains were isolated from the soil and tested for siderophore producing ability. Among siderophore producing bacteria, three superior isolates with the highest level of siderophore (F13, F58 and F66) selected for further study. Production of lecithinase, arginine dehydrolase, catalase, oxidase, hydrogen cyanide, indole acetic acid, solubilization of phosphate, gelatin hydrolysis, oxidative/fermentative, motility and gram staining by these strains was evaluated. In greenhouse experiment, the ability of selected isolates in solubilizing insoluble iron-containing compounds was examined at three moisture levels (0.45–0.6, 0.6–0.7 and 0.7–0.85 field capacity). Results showed that isolates F13 and F66 have greatest effects on chlorophyll amount and shoot dry weight compared to isolate F58. On average, iron concentration of sunflower in the presence of bacteria was 2.85-fold more than control, while it was 1.88-fold for roots. In addition, bacterial treatments enhanced resistance to water stress in sunflower. Based on biochemical tests and by comparison of 16S rRNA sequences, the F13, F58 and F66 isolates were closely related to Pseudomonas spp., Enterobacter spp. and Bacillus Sporothernodurans, respectively.     

Growth response and nitrogen metabolism of sunflower (Helianthus annuus L.) to vermicompost and biogas slurry under salinity stress

Sunflower was grown under saline media with or without vermicompost amendment and biogas slurry, the organic fertilizers. A randomized complete block design with five replications was used. Forty-five pots were divided in three sets comprising of 15 pots each. Out of 15 pots of each sets, five pots of each were subjected to different levels of saline water irrigation i.e. electrical conductivity (EC): 0.5, 4.8 8.6 dS/m). Amendments of vermicompost and biogas slurry have shown some reduction of sodium induced inhibitory effects. Analyses of fresh and dry weight of leaves, ions, amino acid, protein and nitrogen metabolism enzymes in leaves etc., have been undertaken with reference to above-mentioned treatments. Organic manure amendments improved growth yield, nitrate and protein content and decreased the amount of sodium (Na⁺), chloride (Cl^?), ammonium and total amino acid under saline or non-saline condition. Activities of nitrogen (N)-assimilating enzymes i.e. nitrate reducatse (NR, EC 1.6.6.1), nitrite reductase (NiR, EC 1.6.6.4), glutamine synthetase (GS, EC 6.3.1.2) and glutamate synthase (NADH-GOGAT, EC 1.4.1.14) were enhanced to some extent irrespective to non saline or saline condition. Under salinity NADH-glutamate dehydrogenase (GDH, EC 1.4.1.2) activity was stimulated concomitantly with the increasing ammonium contents and proteolysis activity in the leaves and organic manure did not show a significant difference as compared to their respective control. With respect to salt stress, among the two above-mentioned organic manure, vermicompost showed better result in the entire studied parameter as compared to the biogas slurry.     

Effects of biochar and fertilizer management on sunflower (Helianthus annuus L.) feedstock and soil properties

The need for bioenergy is increasing with increase in global energy demand, and sustainable soil and fertilizer management practices for bioenergy feedstock production are gaining importance. In this greenhouse study, we evaluated the effects of biochar and fertilizer nitrogen on soil and energy crop sunflower (Helianthus annuus L. var. Giganteus). Sunflower plants were treated with three rates of biochar, control (0 Mg ha^?1), low (25 Mg ha^?1) and high (50 Mg ha^?1), and three rates of fertilizers, 0% (control), 50% (low) and 100% (high) of the recommended nitrogen dose. Plant height, quality (chlorophyll content), biomass yield, feedstock energy, ash content and tissue nutrients were measured along with soil moisture and pH. Results showed an 11% increase in mean plant height under low biochar compared to control biochar-treated plants. High nitrogen treatment produced 26% and 18% more stalk and total above-ground plant (whole plant) biomass, respectively, compared to the control nitrogen treatment. High biochar treatment resulted in higher soil moisture holding, but lower soil pH than the control biochar treatment. Plant quality, energy and ash contents were not affected by either biochar or nitrogen. The plant tissue analysis provides a complete tissue macro- and micronutrient information on sunflower cultivar Giganteus, which was not done previously.     

Influence of K nutrition and drought on water relations and growth of sunflower (Helianthus annuus L.)

Sunflower plants (Helianthus annuus L. cv. Zwerg Sonnengold) were cultivated in pots containing 1 kg of soil fertilized with 0.6 g K (K₁) and 5.0 g K/pot (K₂). At the age of 5 and 11 weeks, respectively the plants were transferred to a growth chamber. 50% of the plants per K treatment received sufficient watering (controls), the remaining 50% were subjected to water stress until visible signs of severe wilting were observed. At that stage the plants were rewatered. In both growth stages and even under water stress conditions the up to 5 times higher K concentrations in the tissue of K₂ plants caused an increased dry matter production during the experimental periods and a larger total leaf area which was less reduced under water stress than the leaf area of K₁ plants. During continuous watering the water content per leaf area of K₂ plants was on the average 1.5g H₂O dm^?2 (K₁: 1.35g), the water content of older K₂ plants being on the average 1.33g H₂O dm^?2 (K₁: 1.25g). During water stress and subsequent recovery this relation observed between K₂ and K₁ remained constant. In young K₁ plants, however, no intensified loss of succulence was found during severe drought compared with 11-week-old K₁ plants where the water content decreased from 1.2g to 0.6g H₂O per dm² of leaf area. At almost equally high soil water availability stomatal diffusive resistance especially of the older unstressed K₂ plants was higher than in K-deficient plants. Under water stress, the degree of stomatal opening of K₂ plants at first decreased more rapidly, but at equally low soil water potentials diffusive resistance in the leaves of K₂ plants remained lower than in K₁ plants. A relation of the leaf water characteristics, total water potential (4₁), osmotic potential (4₂), and turgor potential (Φ_p) to the corresponding soil water potential showed that under stress Φ₁ and Φ_s in plants with sufficient K nutrition always remained on a higher level than in K₁ plants. Consequently, they were able to maintain a higher turgor pressure even under conditions of restricted water availability.     

Iron nutrition of sunflower(Helianthus annuus L.) as affected by various levels of p and ZN

The effects of various P and Zn levels on iron nutrition of sunflower (Helianthus annuus L.c.v. Record) were studied in two separate experiments in nutrient solution under greenhouse conditions.

In the first experiment, sunflower was grown in nutrient solutions containing four levels of P(1.5, 2.5, 3.5 and 4.5 mM/l) and three levels of Fe(0.25, 0.75, and 1.5 ppm) as FeCl₃ or FeEDDHA. In the second experiment (following the first experiment), the treatments were three P levels (0.75, 1.50 and 3.00 mM/l), three Fe levels (0.25, 0.75 and 1.5 ppm) as FeEDDHA and three Zn levels (0.1, 0.2 and 0.4 ppm).

The plants receiving Fe‐chelate, except for 0.25 ppm Fe, showed no symptoms of iron chlorosis. With inorganic Fe treatments, iron chlorosis appeared after 7–10 days depending on P level, but except for 0.25 ppm Fe which remained chlorotic, plants recovered completely within 3–4 days thereafter due to pH regulating mechanism of sunflower under iron stress condition. With both sources of Fe, chlorosis was associated with high P:Fe ratio.

Increased P and Fe levels in nutrient solution resulted in general increases in the dry weights of roots and shoots. The Fe concentration of shoots, except in few instances, was not affected by P levels, indicating that the sunflower cultivar used in this experiment could utilize inorganic Fe as well as Fe‐chelate under our experimental conditions.

Increasing P levels caused significant increases in Mn content of the shoots as 0.25 and 0.75 ppm inorganic Fe³⁺. Increased Fe levels increased shoot Mn content with inorganic Fe and decreased it with Fe‐chelate. The effects of P, Fe and Zn on sunflower indicated an antagonistic effect of Zn on 1.5 ppm Fe for all P levels. Increased Zn levels in nutrient solution generally increased Zn content of the shoots without having any marked effect on their Mn content.     

Metal contamination effects on sunflower (Helianthus annuus L.) growth and protein expression in leaves during development

Metal-ion contamination (Cd, Cu, Pb, and Zn) on sunflower (Helianthus annuus L.) growth and total leaf protein expression were studied in the present work. The height, mass production, and metal distribution (Ca, K, Fe, Mg, Na, and P) in all plant fractions (roots, stems, and leaves) were evaluated. Sunflowers plants contaminated with four metal ions decreases height and mass by 35% and 40%, respectively, compared to control. Significant differences of total protein composition were noted after SDS-PAGE separation. Sunflower proteomics were more affected when 500 mg L(-1) of metal ion was added as contaminant of both zinc and mixed ions solution. In these cases, proteins having a molar mass of 14.5, 34.5, and 54.0 kDa were present at a lower level and alterations in enzymatic activities (SOD and GR) were found. Sunflowers plants contaminated with zinc and the mixed ions solution showed some degree of oxidative stress.     

Effect of Primary Treated Biomethanated Spentwash on Soil Properties and Yield of Sunflower (Helianthus annuus L.) on Sodic Soil

The field experiment on effect of primary treated biomethanated spentwash (PBSW) on physiochemical and biological properties of soil and yield of sunflower (Helianthus annuus L.) on sodic soil was conducted at the Postgraduate Farm, Mahatma Phule Agricultural University, Ahmednagar, India, during 2008–9. The experiment was laid out in a randomized block design (RBD) with nine treatments [control, varying doses of PBSW (30, 60, 90, 120, 150, and 180 m³ ha^?1), farmyard manure (FYM) + recommended dose of fertilizer (RDF), and FYM + gypsum at 50% gypsum requirement (GR) + RDF] with three replications. The FYM dose was 5 Mg ha^?1. The experimental soil was sodic calcareous, being of the Sawargaon series of isohyperthermic family of Vertic haplustepts with high exchangeable sodium percentage (ESP), low available nitrogen (N) and phosphorus (P), and high available potassium (K). The results revealed that the physical properties [bulk density, mean weight diameter (MWD) of water-stable aggregates, and hydraulic conductivity] of soil were improved in both layers of sodic soil (0–30 and 30–60 cm) as a result of the addition of increased doses of PBSW. The significant reduction in pH, calcium carbonate (CaCO₃), ESP and increase in organic carbon, cation exchange capacity (CEC) and electrical conductivity (EC) were observed in both soil layers as a result of the addition of PBSW at 180 m³ ha^?1. The changes in chemical properties were also seen in the treatment of FYM + GR + RDF, but it was at par with lower doses of PBSW (30 to 90 m³ ha^?1). The microbial populations [bacteria, fungi, actinomycetes, azotobacter, and phosphate-solubilizing bacteria] increased with an increase in the levels of application of PBSW. However, it was maximum in FYM + GR + RDF treatment, and it showed an overall increase up to the flowering stage and thereafter reduced at harvest. The soil basal respiration as mg carbon dioxide (CO₂) increased with the increase in levels of PBSW application but it was the greatest in the FYM + gypsum + RDF treatment. Among the PBSW treatments, the greatest activities of soil enzymes (urease, dehydrogenase, and acid phosphatase) under the treatment of 180 m³ ha^?1 PBSW were observed at the flowering stage. The available N, P, and K after harvest of sunflower crop were significantly greater in the PBSW treatment applied at 180 m³ ha^?1; however, N, P, and K uptake and yield of sunflower were significantly greater in FYM + RDF + gypsum treatment followed by FYM + RDF and 180 m³ ha^?1 of PBSW.     

Assessment of genomic diversity among and within Iranian confectionery sunflower (Helianthus annuus L.) populations by using simple sequence repeat markers

Abstract

Genetic variation in 15 confectionery sunflower populations from different geographical regions of Iran was evaluated using microsatellites. Forty-three alleles were scored at 20 SSR loci. The average number of alleles per locus (na) and the effective allele number (ne) were 2.15 and 1.72, respectively. Observed heterozygosity (0.55) was higher than the expected (0.38) indicating lack of Hardy–Weinberg equilibrium. The genetic similarity matrix for individuals was constructed using Kosman and Leonard similarity coefficient, and Nei's Genetic Identity as well as Kosman's genetic distance was calculated among populations. Dendrograms were constructed by the unweighted pair-group method using arithmetic average (UPGMA) algorithm. The analysis of molecular variance revealed lower genetic variation among populations (14%), than within populations (86%). The average number of migrants (Nm) between populations based on AMOVA (Fst = 0.062) was 4.79. Results displayed high variation among individual plants, possibly because of the high allogamy. Genetic similarity between populations in this study could be considered as consequences of genetic equilibrium that has occurred over the long period of cultivation of confectionery sunflower in this region as well as seed exchange among provinces. The traditional assumption that selecting genotypes of different geographical origin will maximize the diversity available to a breeding project does not hold in confectionery sunflower.     

Relationship between leaf apoplast pH and iron chlorosis of sunflower (Helianthus annuus L.)

A hypothesis has been presented and tested that bicarbonate (HCO₃) and nitrate (NO₃) are the most important anions inducing iron (Fe) chlorosis because these anions increase the pH of leaf apoplast which in turn depresses ferric‐iron [Fe(III]) reduction, and hence, the uptake of Fe into the symplasm. Experiments with young sunflower (Helianthus annuus) plants showed that nutrition with NO₃ as the sole nitrogen (N) source induced chlorosis whereas ammonium nitrate (NH₄NO₃) did not. Monohydrogen carbonate (bicarbonate) also favoured the development of chlorosis. The degree of chlorosis was not related to the Fe concentration in the leaves. Both anion species, NO₃ and HCO₃, increased the pH of the leaf apoplast which was measured by means of the fluorescence dye 5‐carboxyfluorescein. A highly significant negative correlation between leaf apoplast pH and chlorophyll concentration in the leaves (r = ‐0.97) was found. Ferric‐Fe reduction in the apoplast—measured by means of ferrocene—provided evidence that a low leaf apoplast pH, obtained with ammonium (NH₄) supply, favoured the reduction of Fe(III) as compared with a higher leaf apoplast pH obtained with NO₃ supply. These results support the hypothesis tested.     

Studies on Fe deficiency stress response in three cultivars of sunflower (helianthus annuus L.)

Iron deficiency stress was induced in cultivars, PKV‐7237, Morden and EC‐68414 of sunflower (Helianthus annuus L) by first growing them in full nutrient medium and transferring to minus Fe medium after a month. The minus Fe nutrient medium was changed again at 13 and 26 days. The change in pH and the degree of chlorosis were recorded. It was found that the pH decreased rapidly in all cultivers from 6.4 to 3.8 in 5 days. The cv. Morden was found more tolerant to the stress than others. Furthermore, the flavinlike pigments as shown by the yellow coloration of the medium were more intense in PKV than in Korden, and were very much less in EC. It was also noted that the pH reduction was non‐redumptive, a feature observed even after transferring twice to fresh minus Fe medium adjusted to pH 6.4. The results of the experiments showed that as long as Fe absorbed from the pre‐stress medium was available in the root, the chlorosis was not severe; but once it was nearly depleted, chlorosis appeared and persisted for a long time.     

Amelioration in Growth and Physiological Efficiency of Sunflower (Helianthus annuus L.) under Drought by Potassium Application

The present study investigated the induced drought tolerance in sunflower through foliar application of potassium (K) at critical growth stages (head formation or achene filling). Five genotypes of sunflower (G-101, SF-187, Hysun-33, Hysun-38, and 64-A-93) were tested for drought tolerance at ?0.55, ?1.36, and ?1.60 MPa osmotic potential using polyethylene glycol 6000. Hysun-33 showed the highest stress tolerance index as calculated from germination percentage, seedling height, root length, and dry matter. This genotype was further evaluated in the field under drought at head formation or achene filling stages, with or without 1% K foliar application. Treatments were arranged in a randomized complete block design with three replicates. Drought at head formation or achene filling stage significantly decreased biological yield, head diameter, plant height, 1000 achene weight, and achene yield as compared to unstressed control. Potassium application significantly improved all the aforementioned parameters and therefore could be a better strategy for ameliorating drought stress in sunflower.     

Evaluation of sunflower (Helianthus annuus L.) single cross hybrids under heat stress condition

Sunflower is an important oilseed crop, which shows susceptibility to heat stress. In this study, 63 single cross hybrids were evaluated under heat stress condition for 2 years and compared with the two commercial hybrids. Genotype and genotype × environment (GGE) was used to differentiate single cross hybrids on the basis of multiple traits. GGE biplot showed that several single cross hybrids had higher seed yield potential than standard check. Moreover, seed yield per plant (SYP) was related to pollen viability percentage, showing that achene yield was the product of high gametophytic fertility under heat stress. Hybrids having high seed yield potential under heat stress had lower cell membrane injury. GGE biplot for SYP and its components showed that single cross hybrids were characterized into two major groups. Group I was further characterized into two sub group. Group Ia included hybrids with high 100-SW, while group Ib had the hybrids with high number of seeds per head and head diameter. Group II had the hybrids with high kernel weight and kernel to seed ratio. The hybrids could be recommended according to their potential utilization in the seed industry.     

Comparative investigation on the susceptibility of faba bean (Vicia faba L.) and sunflower (Helianthus annuus L.) to iron chlorosis

Comparative physiological studies on iron (Fe) chlorosis of Vicia faba L. and Helianthus annuus L. were carried out. High internal Fe contents in Vicia cotyledons (16–37 μg) were completely used for plant growth and Fe chlorosis was not inducible by the application of nitrate (with or without bicarbonate). In Helianthus, low quantities of Fe in the seeds (4 μg) were insufficient for normal growth and without Fe in the nutrient solution, Fe chlorosis was obtained in all treatments. This chlorosis was an absolute Fe deficiency. Also, the treatment with 1 μM Fe in the nutrient solution and nitrate (with or without bicarbonate) led to severe chlorotic symptoms associated with low leaf Fe concentrations and high Fe concentrations in the roots. In contrast, Helianthus grown with NH₄NO₃ and 1 μM Fe had green leaves and high leaf Fe concentrations. However, with NO₃ supply (with or without bicarbonate), Fe translocation from the roots to the upper plant parts was restricted and leaves were chlorotic. Chlorotic and green sunflower leaves may have the same Fe concentrations, leaf Fe concentration being dependent on Fe translocation into the leaf at the various pH levels in the nutrient solution. At low external pH levels (controlled conditions) more Fe was translocated into the leaf leading to similar leaf Fe concentrations with higher chlorophyll concentrations (NH₄NO₃) and with lower chlorophyll concentrations (NO₃). This indicates a lower utilization of leaf Fe of NO₃ grown sunflower plants. Utilization of Fe in faba bean leaves is presumably higher than in sunflower leaves. In Vicia xylem sap pH was not affected by nitrate. In contrast, the xylem sap pH in Helianthus was permanently increased by about 0.4 pH units when fed with nitrate (with or without bicarbonate) compared with NH₄NO₃ nutrition. The xylem sap pH is indicative of leaf apoplast pH. From our earlier work (Mengel et al., 1994; Kosegarten und Englisch, 1994) we therefore suppose that in Helianthus, Fe immobilization occurs in the leaf apoplast due to high pH levels when grown with nitrate (with or without bicarbonate).