Can phosphorus (P)-releasing bacteria and earthworm (Eisenia fetida L.) co-enhance soil P mobilization and mycorrhizal P uptake by maize (Zea mays L.)?

Journal of Soils and Sediments - Both earthworm (Eisenia fetida L.) and phosphorus (P)-releasing (PR) bacteria, including organic P-mineralizing (OPM) and inorganic P-solubilizing (IPS), are able...     

Effects of epigeic earthworm (Eisenia fetida) and arbuscular mycorrhizal fungus (Glomus intraradices) on enzyme activities of a sterilized soil–sand mixture and nutrient uptake by maize

A pot experiment was conducted to investigate the effect of epigeic earthworm (Eisenia fetida) and arbuscular mycorrhizal (AM) fungi (Glomus intraradices) on soil enzyme activities and nutrient uptake by maize, which was grown on a mixture of sterilized soil and sand. Maize plants were grown in pots inoculated or not inoculated with AMF, treated or not treated with earthworms. Wheat straw was added as a feed source for earthworms. Mycorrhizal colonization of maize was markedly increased in AM fungi inoculated pots and further increased by addition of epigeic earthworms. AM fungi and epigeic earthworms increased maize shoot and root biomass, respectively. Soil acid phosphatase activity was increased by both earthworms and mycorrhiza, while urease and cellulase activities were only affected by earthworms. Inoculation with AM fungi significantly (p?<?0.001) increased the activity of soil acid phosphatase but decreased soil available phosphorus (P) and potassium (K) concentrations at harvest. Addition of earthworms alone significantly (p?<?0.05) increased soil ammonium-N content, but decreased soil available P and K contents. AM fungi increased maize shoot weight and root P content, while earthworms improved N, P, and K contents in shoots. AM fungi and earthworm interactively increased maize shoot and root biomass through their regulation of soil enzyme activities and on the content of available soil N, P, and K.     

Earthworm (Aporrectodea trapezoides)–mycorrhiza (Glomus intraradices) interaction and nitrogen and phosphorus uptake by maize

The interactive impacts of arbuscular mycorrhizal fungi (AMF, Glomus intraradices) and earthworms (Aporrectodea trapezoides) on maize (Zea mays L.) growth and nutrient uptake were studied under near natural conditions with pots buried in the soil of a maize field. Treatments included maize plants inoculated vs. not inoculated with AMF, treated or not treated with earthworms, at low (25 mg kg⁻¹) or high (175 mg kg⁻¹) P fertilization rate. Wheat straw was added as feed for earthworms. Root colonization, mycorrhiza structure, plant biomass and N and P contents of shoots and roots, soil available P and NO₃⁻–N concentrations, and soil microbial biomass C and N were measured at harvest. Results indicated that mycorrhizal colonization increased markedly in maize inoculated with AMF especially at low P rate, which was further enhanced by the addition of earthworms. AMF and earthworms interactively increased maize shoot and root biomass as well as N and P uptake but decreased soil NO₃⁻–N and available P concentrations at harvest. Earthworm and AMF interaction also increased soil microbial biomass C, which probably improved root N and P contents and indirectly increased the shoot N and P uptake. At low P rate, soil N mobilization by earthworms might have reduced potential N competition by arbuscular mycorrhizal hyphae, resulting in greater plant shoot and root biomass. Earthworms and AMF interactively enhanced soil N and P availability, leading to greater nutrient uptake and plant growth.     

Rediscovering ‘Mexican June’: a nearly extinct landrace maize (Zea mays L.) variety

Genetic Resources and Crop Evolution - ‘Mexican June’ was a popular white, open-pollinated maize variety grown in the southwestern and southern USA. It was well adapted to both...     

Farmers’ adoption of maize (Zea mays L.) hybrids and the persistence of landraces in Southwest China: implications for policy and breeding

This paper examines changes in the distribution of maize hybrids and landraces in the mountainous areas of southwest China over 1998–2008, farmers’ reasons for cultivar adoption and the implications for national policies in relation to seed production and breeding, based on baseline data and a survey conducted in Guangxi, Yunnan and Guizhou. The study traced the dynamic changes in the adoption of hybrids and landraces in farmers’ fields, explored how individual farmer’s choices can influence local landrace distribution, and investigated the space for conducive policy and innovative action for on-farm conservation of maize genetic resources. The research showed that although there is strong farmers’ interest in accessing modern maize hybrids, farmers also express strong reasons for maintaining at least some of the landraces that satisfy local agronomic context and social preferences. Farmers recognized that hybrids have a number of advantages but they also indicated some disadvantages of the current available hybrids e.g. with respect to seed quality, local adaptability, taste and cost of seeds, but also lack of information on the performances of the new hybrids. Based on farmers’ reasoning and experiences, the requirements have been identified for improving yield combined with local preferences (agronomic, cultural and socio-economic). The paper concludes by identifying options for how China might seek to develop resilient seed systems for smallholder farmers in poor areas, under changing climatical conditions and volatile markets. Participatory Plant Breeding is among the options considered for bringing farmers’ needs into conservation and breeding strategies for improving local adaptation.     

Characterization of “Mais delle Fiorine” (Zea mays L.) and nutritional,morphometric and genetic comparison with other maize landraces of Lombardy region (Northern Italy)

Genetic Resources and Crop Evolution - The loss of agrobiodiversity is a topic of global impact. On a local scale, Lombardy, in the Alpine macro-Region, has lost more than 78% of its plant...     

The effect of an NH4 +‐enhanced nitrogen source on the growth and yield of hydroponically grown maize (Zea mays L.)

Increased above‐ground dry matter and grain yields were found for two hydroponically grown maize hybrids (Pioneer‐3925 and Pioneer‐3949) when plants were supplied with an NH₄ ⁺‐enhanced nutrient solution (31 percent of total N) compared with a control (4 percent of total N as NH₄ ⁺). The major difference in yield developed between silking and 2 weeks post‐silking and silking and 4 weeks post‐silking for the P‐3925 and P‐3949 respectively. The reduced nitrogen content of the stover (leaves plus stalk) was consistently higher on the NH₄ ⁺‐enhanced nutrient solution. The decreased production of the control treatment may have resulted from a reduced photsynthetic capacity.     

Different Forms of Potassium and Their Contributions toward Potassium Uptake under Long-Term Maize (Zea mays L.)–Wheat (Triticum aestivum L.)–Cowpea (Vigna unguiculata L.) Rotation on an Inceptisol

In a long-term fertilizer experiment at the Indian Agricultural Research Institute, New Delhi, with maize, wheat, and cowpea, various forms of potassium (K) and their contribution toward K uptake were found to be affected by fertilizer use and intensive cropping. The treatments included for the study were a control, 100% nitrogen (N), 100% N–phosphorus (P), 50% NPK, 100% NPK, 100% NPK + farmyard manure (FYM at 15 t ha^?1 to maize only), and 150% NPK. The concentration of nonexchangeable K was greatest, followed by exchangeable K and water-soluble K. The study revealed no significant change in water-soluble K concentration in surface soil compared to N, NP, and control, indicating existence of an equilibrium between different K forms. Application of 100% NPK significantly increased water-soluble K concentration in surface soil compared to N, NP, and control treatments after maize, wheat, and cowpea. Application of NPK + FYM and 150% NPK resulted in greater quantities of all the K forms as compared to other treatments. Among the three forms, water-soluble K contributed predominantly to K uptake by maize and wheat; however, nonexchangeable K contributed significantly to K uptake by cowpea.     

Evaluation of urea ammonium polyphosphate (UAPP) I. Effectiveness of banded and seed‐placed UAPP on irrigated corn (Zea mays L.)

Abstract

Field trials were established on an irrigated loamy sand using corn as a test crop. Row fertilizer was placed with the seed (seed‐placed) or in the conventional side placement position (side‐placed). The side‐placed treatments consisted of two carriers of UAPP (28–28–0 and 36–18–0), diammonium phosphate (DAP, 18–46–0) plus ammonium nitrate (AN, 33–0–0), and a control treatment. Two rates of application were used for each carrier. Seed‐placed treatments consisted of two carriers of UAPP (28–28–0 and 36–18–0), concentrated superphosphate (CSP, 0–45–0) plus AN, CSP, AN, and a control treatment. Two rates of application were used for each carrier, except AN, which was used at three rates.

High rates of side‐placed UAPP delayed emergence of corn and lowered the final population. Early season N concentration in corn seedlings was not affected by rate or carrier of side‐placed N. Early season P concentration in the corn seedlings was greater for AN + DAP than UAPP treatments at all rates of P, presumably due to toxic accumulation of free NH₃ in the UAPP band. High rates of side‐placed UAPP also reduced the yield of corn.

High rates of seed‐placed AN + CSP and UAPP reduced the germination of corn. However, low rates of UAPP also reduced germination, whereas low rates of AN + CSP did not affect germination. Since UAPP has a lower salt index than AN + CSP, the “salt effect”; of low rates of UAPP does not explain its effect on germination and emergence. The deleterious effects of UAPP were due apparently to either NH₃ or NO₂‐N toxicity.     

Effects of benomyl on vesicular‐arbuscular mycorrhizal infection of red clover (Trifolium pratense L.) and consequences for phosphorus inflow.

The roots of red clover plants inoculated with the vesicular‐arbuscular mycorrhizal fungus Glomus clarus Nicolson & Schenck were treated with a drench of benomyl at a time when mycorrhizal infection was already well established. Benomyl halted further infection and reduced phosphorus inflow by one order of magnitude compared to untreated controls. The fungicide also decreased the rate of plant growth probably as a consequence of reduced phosphorus inflow.     

Transfer of rubidium‐86 (potassium) from deeply‐rooted alfalfa (Medicago sativa L.) to associated,shallow‐rooted maize (Zea mays L.) or grain sorghum (Sorghum vulgare pers.).

Circumstantial evidence exists for non‐N‐mineral element transfer in legume‐grass associations. Three experiments were conducted in an effort to directly demonstrate transfer of a non‐N‐mineral element in alfalfa (Medicago sativa L.)‐maize (Zea mays L.) and alfalfa‐grain sorghum (Sorghum vulgare Pers.) associations in two rooting media. Associations were established in double‐tube apparatus so that a single alfalfa plant was rooted in media of top‐ and bottom‐tubes, while an associated grass plant was rooted exclusively in the top‐tube (Intact treatment). Severed treatments (the control) were identical to the Intact treatments except the alfalfa roots in an air gap between the top‐and bottom‐tubes were excised.

⁸⁶Rb was dispensed onto the medium of bottom tubes with movement of the radioisotope determined by analyzing the legume and grass tissues over time. ⁸⁶Rb was detected in: i) soil‐grown maize associated with alfalfa within a 40‐day treatment period; ii) sand‐grown maize associated with alfalfa within 20 days after treatment and iii) sand‐grown sorghum associated with alfalfa within 10 days. Detection of ⁸⁶Rb in grass plants associated with alfalfa demonstrated that transfer of this potassium analog can occur via the root systems of legume‐grass associations.     

Can We Reduce Rainfed Maize (Zea mays L.) Nitrogenous Fertilizer Application Rate with Addition of Nitrapyrin?

Application of nitrification inhibitor has potential to increase soil nitrogen (N) retention throughout the growing season and finally increase corn (Zea mays L.) yield. During the 2012–2014 growing seasons, on-farm field trials were conducted to determine the effects of nitrapyrin (Instinct) with two N sources, urea and urea ammonium nitrate, at two rates, 85% and 100% of recommended N, and side-dress on grain yield and soil inorganic N availability in the Red River Valley of the North Dakota. Preplant urea N at 100% recorded the greatest yield in 2 out of 3 years. At late sampling, the greatest soil inorganic N was observed with side-dress urea ammonium nitrate at 100% within 0–30 cm (last 2 years). For spring fertilizer N management, addition of nitrapyrin had no effect on yield and inconsistent effect on soil N availability. Our results suggest that fertilizer N management should be evaluated on a local scale and consider annual variability in weather.     

Effects of Conjunctive Nutrient Management on Soil Fertility and Overall Soil Quality Index in Submountainous Inceptisol Soils under Rainfed Maize (Zea mays L.)–Wheat (Triticum aestivum) System

The present long-term study was initiated to quantify the long-term effects of conjunctive nutrient management on soil quality, identify key indicators, and assess soil quality indices under a rainfed maize–wheat system in marginal Inceptisol soils in India. Results of the study revealed that soil organic carbon was significantly influenced by the conjunctive nutrient-management treatments. Among the nine treatments, the application of 100% recommended dose of nitrogen (RDN) (80 kg N ha^?1), 15 kg N (compost) + 20 kg N ha^?1 (inorganic), 25 kg N (compost), and 15 kg N (compost) + 10 kg N ha^?1 (green leaf) resulted in greater organic carbon contents of 5.57, 5.32, 5.27, and 5.26 g kg^?1, which were greater by 29.5%, 24%, 23%, and 22%, respectively, over the control. The greatest soil quality index (1.61) was observed with application of 25 kg nitrogen (N; compost) as well as with application of 15 kg N (compost) + 10 kg N ha^?1 (green leaf). The order of percentage contribution of key indicators toward soil quality indices was available potassium (K) (34%) > available phosphorus (P) (32%) > available N (13%) > microbial biomass carbon (12%) > exchangeable calcium (Ca) (9%). The linear regression equation revealed the principal role of soil quality indicators in maize crop yield. The methodology and the results of the study could be of great relevance in improving and assessing soil quality not only for the study locations but also for other climatically and edaphically identical regions across the world.     

Contribution of Roots and Mycorrhizal Hyphae on Phosphorus Efficiency of Maize (Zea mays,L.) Genotypes Grown on Calcareous Soil—A Mechanistic Modeling Approach

ABSTRACT

This work was conducted to study phosphorus (P) efficiency of two maize genotypes (Zea mays, L.) in calcareous soil grown in potted soil with two levels of P in soil by adding 40 and 270 mg P/kg soil. Half of the pots were inoculated with arbuscular mycorrhizal fungi (AMF) (Rhizoglomus irregulare). The maize genotypes were harvested two times at 35 and 50 days after transplanting. The plant dry matter, root length and Plant P uptake of maize genotype Hagen 1 without mycorrhizal fungi (AMF) increased significantly compared with Hagen 9 at a low P level. In contrast, there was no significant difference between two maize genotypes inoculated with AMF under the same P level. The predicted value increased rapidly with increasing P levels from about 70% up to 97% in both maize genotypes with and without mycorrhizal fungi. At a low P level, the mycorrhizae hyphae contributed by about 31.6% and 30.2% of the predicted total P uptake in maize genotype Hagen 1 and Hagen 9, respectively. The results of this study suggested that the P-inefficient genotype Hagen 9 improved with inoculation with mycorrhizal fungi under a low P level at the same conditions of this experiment. Also, root growth system and mycorrhizal hyphae length would be a suitable plant parameter for studying P efficient maize genotypes, especially under limited P supply. The current study clearly pointed out that the mechanistic simulation model (NST 3.0) provides useful tools for studying the role of AMF in P uptake of plant.     

Analysis of volatile compounds of taperebá (Spondias mombin L.) and cajá (Spondias mombin L.) by simultaneous distillation and extraction (SDE) and solid phase microextraction (SPME)

Volatile compounds of cajá and taperebá fruits, both classified as Spondias mombin, but from different geographic origins, were extracted (and analyzed) using solid phase microextraction (SPME) and simultaneous distillation and extraction (SDE). Forty-eight compounds were identified in taperebá and 47 in cajá by SPME using a DVB/CAR/PMDS fiber. (E)-Caryophyllene (18.7%), ethyl butyrate (10.0%), and ethyl hexanoate (7.0%) were the most abundant components in taperebá volatiles extracted by SPME, whereas myrcene (41.1%) and beta-phellandrene (8.5%) were the major compounds in cajá. In the taperebá SDE extract, 46 substances were identified, and (Z)-caryophyllene (13.2%) and limonene (9.5%) were predominant. From the 42 substances found in the SDE extract of cajá, the major components were myrcene (38.0%) and p-cymene (6.2%). The two fruits showed similar chromatograms upon the use of SDE and SPME. These methods made it possible to determine 30 identical components in both fruits by using SDE and 32 by using SPME.     

Effects of a ‘one film for 2 years’ system on the grain yield,water use efficiency and cost-benefit balance in dryland spring maize (Zea mays L.) on the Loess Plateau,China

‘One film for 2 years’ (PM2) has been proposed as a practice to control the residual film pollution; however, its effects on grain-yield, water-use-efficiency and cost-benefit balance in dryland spring maize production have still not been systematically explored. In this study, we compared the performance of PM2 with the annual film replacement treatment (PM1) and no mulch treatment (CK) on the Loess Plateau in 2015–2016. Our results indicated the following: (1) PM2 was effective at improving the topsoil moisture (0–20 cm) at sowing time and at seedling stage, but there was no significant influence on soil water storage, seasonal average soil moisture or evapotranspiration; (2) PM2 induced significantly higher cumulative soil temperatures compared to CK, and there was no significant difference between PM2 and PM1; (3) no significant differences were identified in grain-yield and water-use-efficiency between PM1 and PM2, and compared to CK, they improved by 16.3% and 15.5%, respectively; (4) because of lower cost of plastic film, tillage, film laying and remove in PM2, economic profits improved by 21% and 70% compared to PM1 and CK. This research suggested that PM2 was effective at alleviating the spring drought and was beneficial in reducing poverty traps in dryland.     

Chemical Changes and Heavy Metal Partitioning in an Oxisol Cultivated with Maize (Zea mays, L.) after 5 Years Disposal of a Domestic and an Industrial Sewage Sludge

The need for solutions to minimize the negative environmental impacts of anthropogenic activities Fhas increased. Sewage sludge is composed of predominantly organic matter and can be used to improve soil characteristics, such as fertility. Therefore, its application in agriculture is an adequate alternative for its final disposal. However, there is a lack of information on its long-term effects on soil changes in tropical areas. Thus, the objectives of this study were to determine (i) the effect of sewage sludge application on heavy metal build-up in soil and maize grains and leaves, and (ii) the effects of soil amendment with sewage sludge on the chemical properties of a Brazilian oxisol. Besides the increasing levels of Zn, Cu, Ni, and Cr, amending soil with sewage sludge also alters the distribution of these metals by increasing the mobile Phases, which correlated significantly with the increase in metal extraction with two single extractants, Mehlich 1 and DTPA (Diethylene triamine pentaacetic acid). The levels of Fe, Mn, Zn, and Cu in maize grains and leaves increased with the type and rate of sewage sludge application. Nevertheless, metal build-up in soil and plants was within the allowed limits. Significant differences were also found in soil characteristics like humic fractionation with the applied sewage doses. The data obtained does not indicate any expressive drawbacks in the use of sewage sludge as a soil amendment, as the heavy metal concentrations observed are unlikely to cause any environmental or health problems, even overestimated loadings, and are in accordance with the Brazilian regulations on farming land biosolid disposal.     

Agronomic, chemical and genetic variability of saffron (Crocus sativus L.) of different origin by LC-UV–vis-DAD and AFLP analyses

The identification of a bi-univocal correspondence between geographical origin of saffron (Crocus sativus L.) and the composition of its stigmas has recently been the subject of many research papers, which have focused on the analysis of the differences among the so called “minor components”, such as flavonoids and volatiles, in the secondary metabolic pattern of this spice. Saffron pigments (crocetin esters), on the other hand, constitute the majority of the metabolites found in its stigmas, and their spectrophotometric measurement is still used as an official method to determine the quality of the spice in terms of coloring power. To our knowledge, no attempts have been made to find a correspondence between the geographical origin of different saffron samples and their morphological traits and pigments pattern. In this paper, we have demonstrated that saffron corms of different origins, grown in the same experimental field, produce daughter corms with different dimensions and still produce stigma samples with different pigment profiles. Furthermore, daughter corm dimensions and pigment profile even more so, may be related to the origin of the sample, and therefore pigments can be used as chemotaxonomic markers. Compositional analyses results were corroborated by genetic data obtained using AFLP molecular markers.