The effect of zinc (Zn) application on uptake of cadmium (Cd) in some cereal species

It is widely accepted that plants absorb more Cd when they suffer from Zn deficiency, as the Zn deficiency is a critical problem in the world. The effect of increased Cadmium (Cd) application (0 and 15 mg kg^-1 Cd) on growing and Cd concentration in some cereal species was investigated at the increasing rate of Zinc (Zn) applications (0 and 15 mg kg^-1 Zn). The experiment was carried out under greenhouse conditions using a Zn deficient soil. According to the results, that increased Cd applications severely reduced dry matter production of plants. Dry matter production decreased in the following order: bread wheat > oat > maize > barley. These decreases were determined to be statistically significant (P < 0.01). Cd concentration of cereal species increased with the application of increasing rate of Cd, with a similar trend to that of the dry matter. Dry matter production of plants increased and Cd concentrations of plants decreased with the application of increasing rate of Zn. These results show that Cd accumulation of plant increase in Zn deficient soils. Cd accumulation in plants is hindered with the application of Zn.     

Characterization of Zinc Oxide Nano Particles and Their Effect on Growth of Maize (Zea mays L.) Plant

In the current literature, the impact of nano-particles (NPs) on growth of higher plants has scantly been reported. An investigation was carried out to study the effect of zinc oxide nano-particles (<100 nm) on growth of maize (Zea mays L.) plant, as one of the major agricultural crops, in a solution culture system. Various concentrations of zinc (Zn) were applied through nano-zinc oxide (ZnO) particles (<100 nm) in suspension form and in ionic form through zinc sulfate (ZnSO₄) salt in Hoagland solution culture. Experimental results showed that nano zinc oxide particles could enhance and maintain the growth of maize plant as well as conventional Zn fertilizer (as ZnSO₄). The plant parameters like plant height, root length, root volume, and dry matter weight were all improved due to application of zinc oxide nano-particle. These findings indicate that plant roots might have the unique mechanism of assimilating nano-Zn and using for its growth and development. Different enzymatic activities were also studied and experimental results revealed that nano-ZnO particles (<100 nm) also governed the enzymatic activity of maize plant. A separate laboratory experiment was also carried out to characterize the zinc oxide nano particle for its size, zeta potential, etc.     

Effect of Pyrite Application on Wheat-Maize Growth and Nutrient Uptake Under Diverse Soil Conditions

Zinc (Zn) and phosphorus (P) deficiency is a common nutritional problem for the production of many crops in semi-arid Mediterranean region of Turkey. This problem results in the application of increasing amounts of fertilizers. Minerals (such as pyrite) including iron (Fe) and sulfur (S) can decrease soil pH may be a critical factor in crop production under low supply of Zn and P in calcareous and clay soils. The aim of this research was to determine the effect of pyrite application on wheat-maize-wheat growth, P and Zn concentration with three successive pot experiments. Bread (Seri-82) (Triticum durum L.) durum (Kunduru) wheat (Triticum aestivum L.), and maize (Zea mays L.) RX 788 hybrid was grown in Zn and P-deficient calcareous soils from Central Anatolian Sultanönü and Çukurova Karaburun. Plants were grown under greenhouse conditions at five rates of pyrite (0, 0.5, 1, 1.5, and 2 g pyrite kg^?1 soil) in three consecutive experiments. Pyrite application increased shoot dry matter production of wheat and maize. With time effect of pyrite on plant growth and nutrient uptake was more. In accordance with growth data, pyrite application enhanced P and Zn concentration of plants, especially under Zn deficient Sultanönü soil then Karaburun soil. Plants grown in Karaburun soil had more P and Zn concentration than grown in Sultanönü soil. The results obtained indicate that pyrite can be used as a zinc fertilizer sources for gramine plants such as wheat and maize.     

Zinc bioavailability in maize grains in response of phosphorous–zinc interaction

Phosphorous (P) and zinc (Zn) are plant nutrients that interact with each other in soil–plant systems. Such interactions may cause deficiency of one of the nutrients interacting with each other if interactions are antagonistic. In the present trial, a field experiment was conducted to investigate the interactive effect of Zn (0 and 16 kg ha^?1) and P (0 and 60 kg ha^?1) on growth, yield and grain Zn concentration of two maize (Zea mays L.) genotypes, i.e., Neelam (local) and DK‐6142 (hybrid). Growth and yield of both maize genotypes were increased by the application of Zn and P treatments compared with control, but Zn+P was more effective than their sole application. When compared to control, combined application of Zn+P increased grain Zn and P concentrations by 52% and 32%, respectively, averaged for the two genotypes. Single application of P decreased grain Zn concentration by 10% over control. Application of P and Zn particularly in combination decreased the grain [phytate] : [Zn] ratio and increased the estimated human Zn bioavailability in grains based on a trivariate model of Zn absorption in both maize genotypes. Conclusively, combined Zn+P application appeared more suitable for enhancing grain yield and agronomic Zn biofortification in maize grains. However, Zn fertilization aiming at increasing grain yield and grain Zn concentration should consider the genotypic variations and P rate.     

Effects of humic acid and Ca(NO3)2 on nutrient contents in pepper (Capsicum annuum) seedling under salt stress

This study was carried out in order to determine the effects of calcium nitrate (Ca(NO₃)₂) and humic acid (HA) applications on nutrient uptake of pepper seedling under salt stress in a plant growth room. Before sowing Demre variety of pepper seeds, 60?mM NaCI was added to each 300?cm³ pot. The experiment was ended at eighth week after the sowing. Applications of HA significantly affected K, Ca, Fe, Mn, Zn (p?p?3)₂ applications did not show a significant effect on Fe and Cu contents of pepper seedling. However, Ca(NO₃)₂ applications significantly affected N and P contents (p?<.05) and K, Ca, Mn, and Zn contents (p?相似文献   

Nitric oxide improves high zinc tolerance in maize plants

A short-term experiment was carried out to study the effects of exogenous nitric oxide (NO) on some growth parameters and mineral nutrients of maize grown at high zinc (Zn). Maize seedlings were planted in pots containing perlite and subjected to 0.05 or 0.5 mM Zn in nutrient solution. Nitric oxide (0.1 mM) was sprayed to the leaves of maize seedlings. High Zn reduced total dry matter, chlorophyll (Chl.) content and leaf relative water content (RWC), but increased proline content and membrane permeability. Foliar application of NO significantly increased chlorophyll content, RWC and growth of plants treated with high Zn, and significantly reduced their membrane permeability and proline contents. High Zn resulted in increased leaf and root Zn, but lower concentrations of leaf phosphorus (P), and iron (Fe). Foliar application of NO lowered leaf and root Zn and increased leaf and root nitrogen (N) and leaf Fe in the high Zn plants. These results clearly demonstrated that externally-applied NO induced growth improvement in maize plants was found to be associated with reduced membrane permeability under high zinc. Results can be concluded that NO may be involved in nutritional and physiological changes in plants subjected to high Zn.     

EFFECT OF LONG-TERM SEWAGE SLUDGE APPLICATION ON THE DISTRIBUTION OF NUTRIENTS IN MAIZE (ZEA MAYS L.)

The distribution of the nutrients nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and iron (Fe) in maize (Zea mays L.) was studied in a long-term sewage sludge field application in an acid coarse sandy soil at Bordeaux, France. Sewage sludge had been applied since 1974 at 100 t (dry weight) ha^?1 per 2 years (SS 100) in plots cultivated annually with maize. Treatment with farmyard manure (FYM) at 10 t (dry weight) ha^?1 per year and mineral fertilization served as control. Five plants per treatment were investigated at six different growth stages. The plants were separated into their different organs and the distribution of nutrients was determined in up to 12 different plant parts. Sludge application did not significantly influence the nutrient partitioning in maize, but improved slightly the plants' nutrient status compared with the FYM treatment. Grain yield was similar for both treatments. The values for N, P, K, Ca, Mg and Fe content in sludge-treated plants were in the recommended range for optimal growth. Thus, sewage sludge would be a valuable source for maize nutrition even after long-term application, if the critically high copper (Cu) and zinc (Zn) concentrations, previously reported in these sludge-amended plants, could be avoided by the use of sludge low in these elements.     

Impacts of biochar and processed poultry manure,applied to a calcareous soil,on the growth of bean and maize

Direct use of poultry manure on agricultural lands may cause environmental concerns, so there is a need to establish the suitability of the application of biochar derived from poultry manure for calcareous soil chemical properties and plant growth. The purpose of this study was to evaluate the effects of processed poultry manure (0, 5, 10 and 20 g/kg) and its biochar (0, 2.5, 5, 10 and 20 g/kg) on soil chemical properties of a calcareous soil and growth of bean (Phaseolus vulgaris) and maize (Zea mays) plants. In the incubation experiment, both processed poultry manure (PPM) and biochar decreased pH and the concentration of plant‐available Fe of soil but increased plant‐available P, Zn, Cu and Mn concentrations. PPM and biochar increased the concentrations of exchangeable cations (K, Ca and Mg) in soil. PPM and biochar applications increased the growth of maize and bean plants. PPM and biochar resulted in increased concentrations of N, P, K, Ca, Fe, Zn, Cu and Mn in bean plants. In maize plants, PPM and biochar applications increased the N, P, K, Zn, Cu and Mn but decreased the Ca and Mg concentrations. Results of this study reveal that poultry manure biochar can be used effectively for agricultural purposes.     

Nitrogen fertilization does not affect micronutrient uptake in grain maize (Zea mays L.)

Abstract

Due to continuous single nitrogen fertilization, we hypothesized a built-up deficiency of micronutrients in crops that would limit plant growth and crop quality. In 2-year field experiments using urea-N fertilized grain maize (Zea mays L.), hybrid KWS 2376 at 0, 120 and 240 kg N ha^?1 crop uptake of Zn, Mn, Cu and Fe was studied at DC 32, DC 61 and in the grain harvested. Micronutrient contents at DC 32 stage – 1st node (aboveground phytomass) and DC 61 – flowering (ear leaf) were all at levels indicative of adequate micronutrient supply to the crop. At both sampling occasions the Fe:Zn and Fe:Mn ratios were adequate implying that Fe did not inhibit the uptake of Zn and Mn. The application of nitrogen increased the Fe content at the 1st sampling in both years; in the second year the same was also the case for the Zn content. Nitrogen nutrition increased the contents of Mn and Fe at the 2nd sampling only in year 2; in the other treatments no changes were observed in the micronutrient contents. Micronutrient correlations in the grain were discovered between Zn and Mn contents and between Fe and Mn contents. In the second year the highest N-rate significantly increased the Fe and Zn content of the grain compared with the lower rates of nitrogen fertilization. Grain yields were not affected by the rate of nitrogen and ranged between 13.65 and 14.34 t ha^?1 (1st year) and between 13.68 and 14.18 t ha^?1 (2nd year). Nitrogen fertilization did not reduce the content of micronutrients in the plant or grain of maize. It is evident that the continuous single use of N fertilization so far has not resulted in a micronutrient deficiency of the plants limiting the nutrient density of the grain or reducing its quality.     

Influence of potassium and magnesium fertilizer application on the yield and nutrient accumulation of maize genotypes under field conditions

The use of maize (Zea mays L.) genotypes that are able to utilize nutrients efficiently is an important strategy in the management of plant nutritional status; it is of particular importance with regard to potassium (K) and magnesium (Mg), due to their high requirement and influence on plant growth. The influence of K and Mg fertilizers on certain growth parameters of maize genotypes TM.815 and KL.72.AA, including length, seed in ear, seed weight growth, and nutrient concentration, was determined under field conditions over two successive years. The aim of the experiment was to study the effect of different rates of K and Mg fertilizers on maize genotype plant growth parameters, grain yield, and nutrient accumulation under field conditions.

A split plot design with three replicates was used and each block contained three treatments of 0, 100, and 200 kg ha^?1 of K₂O and 0, 10, and 20 kg ha^?1 of Mg; K₂SO₄ was used to supply K, and MgSO₄ was used for Mg.

Plants that responded to the K fertilizer had an increase in height, yield, and the concentration of K in the leaves and seeds. The addition of K fertilizer increased the concentration of nitrogen (N), iron (Fe), zinc (Zn), manganese (Mn), and K in the plant leaves and increased seed K concentration. Mg fertilizer increased the concentration of N, Fe, copper, and Mn in the leaves; however, it exerted no significant influence on K concentration. The KL.72.AA maize genotype had a higher mean plant height, number of seeds in ear, yield, and N, K, Fe, and Zn concentrations compared to the TM.815 maize genotype. In the experiment, the K fertilizer exerted a statistically significant effect on the leaf and seed K concentration; however, on a statistical basis, the Mg fertilizer did not affect the Mg concentration.     

Total Chromium Captured by Maize (Zea Mays) Plants is Increased by Phosphate and Iron Supplementation in the Soil

In this work, a commercial hybrid maize was used, and the effect of selected mineral nutrients [sulfur (S), phosphorus (P), iron (Fe), and nitrogen (N)] on the chromium (Cr) captured by plants was analysed. Seeds were germinated in vitro, and plants were transferred to pots with soil supplemented with a growth inhibitory Cr concentration and different mineral nutrient combinations. Plants were grown for 13 weeks and irrigated with water. In the harvested plants, total Cr was determined considering the Cr concentration in tissues and the biomass as dry weight. The soil supplemented with phosphorus (P) or Fe improved the total Cr captured by plants by a quarter. Bioconcentration values in plant tissues were similar to those of other plants grown in soil with Cr. This information will be useful either for Cr phytoremediation or to protect commercial maize crops cultivated in Cr contaminated soils.     

Yield and Zinc,Copper, Manganese and Iron Concentration in Maize (Zea mays L.) Grown on Vertisol as Influenced by Zinc Application from Various Zinc Fertilizers

Zinc (Zn) deficiency in soils and field crops is widespread across the world, including India, resulting in severe reduction in yield. Hence, soil application of Zn fertilizers is recommended for ameliorating Zn deficiency in soil and for obtaining higher crop yield and better crop quality. Zinc sulfate is commonly used Zn fertilizer in India because of its solubility and less cost. However, good quality and adequate quantity of zinc sulfate is not available in the market round the year for farmers' use. Field experiments were therefore conducted during rainy season of 2010 and 2011 at research farm of Indian Institute of Soil Science, Bhopal, India to assess the influence of Zn application through zinc sulfate monohydrate (33% Zn), zinc polyphosphate (21% Zn) and Zn ethylenediaminetetraacetate (EDTA) (12% Zn) on yield and micronutrient concentration and uptake by maize (Zea mays L.). In both the years, grain and vegetative tissue (stover) yield of maize increased significantly with successive application of Zn up to 1 kg ha^?1 added through zinc sulfate monohydrate and zinc polyphosphate. Addition of 2.5 kg Zn ha^?1 did not increase yield further but resulted in highest stover Zn concentration. Zinc, copper (Cu), manganese (Mn), and iron (Fe) concentration in maize grain varied from 22.2 to 27.6, 1.6 to 2.5, 3.5 to 4.7 and 19.9 to 24.5 mg kg^?1 respectively in both the years. Maize stover had 25.9 to 36.2, 7.9 to 9.8, 36.7 to 44.9 and 174 to 212 mg kg^?1 Zn, Cu, Mn, and Fe, respectively. Zinc application did not influence Cu, Mn and Fe concentration in both grain and stover of maize. Transfer coefficients (TCs) of micronutrients varied from 0.72 to 0.95, 0.18 to 0.30, 0.08 to 0.13 and 0.10 to 0.15 for Zn, Cu, Mn, and Fe respectively. Total Zn uptake significantly increased with Zn application from 0.5 to 2.5 kg ha^?1 supplied through zinc sulfate monohydrate and zinc polyphosphate. Recovery efficiency of Zn declined with increased Zn rates.     

Foliar application of iron,zinc, and manganese nano-chelates improves physiological indicators and soybean yield under water deficit stress

Abstract

To investigate the effect of foliar application of nano-chelates of iron, zinc, and manganese subjected to different irrigation conditions on physiological traits, and yield of soybean (cultivar M9), a split plot experiment was conducted in a completely randomized block design with three replications in two crop years (2016–2017). The main plot included four levels of irrigation (I): full irrigation (I ₁), irrigation withhold at flowering stage (I ₂), irrigation withhold at podding stage (I ₃), and irrigation withhold during the grain filling period (I ₄). Also, the subplot included eight levels of foliar application with Fe, Zn, Mn, Fe?+?Zn, Fe?+?Mn, Zn?+?Mn, Fe?+?Zn?+?Mn nano-chelates, and distilled water (control). The results of combined analysis of variance suggested that the effect of irrigation and foliar application of nano-chelate was significant on all traits. Water deficit stress significantly reduced the grain yield. The minimum numbers of pods per plant, number of grains per plant, 100-seed weight per plant, leaf area index, leaf chlorophyll concentration, total dry weight of plant, and the grain yield were obtained by irrigation withhold at podding stage. Foliar application of combined nano-chelates increased the soybean resistance against water shortage more considerably than the separate consumption of these elements. Under drought stress in podding stage, the application of Fe?+?Zn led to the highest yield with a mean of 2613.84?kg ha^?1 where this increase was 61.1% higher than control.     

不同水分状况下施锌对玉米生长和锌吸收的影响

选择潮土(砂壤)和土(粘壤)两种质地不同的土壤,进行盆栽试验,研究不同土壤水分条件下施锌对玉米生长和锌吸收的影响。结果表明,施锌显著增加了玉米植株根、茎、叶以及整株干物质重;缺锌条件下玉米植株根冠比、根叶比和根茎比趋向增大。施锌显著提高了玉米植株各器官中锌的浓度和吸收量,并明显促进锌向地上部运移。干旱胁迫抑制了玉米植株生长,根冠比、根茎比、根叶比增大;随着土壤水分供应增加,植株生长加快,各器官生物量以茎和叶增加大于根。水分胁迫下,在潮土上玉米叶片中锌浓度上升;在土上叶片中锌浓度下降。但增施锌后,根和茎锌浓度增加幅度较大,叶片增加幅度较小;施锌和水分胁迫对根和茎锌浓度的交互作用极显著。水分胁迫下,玉米植株对锌的吸收总量减少。水分胁迫和锌肥施用对玉米叶片、茎锌吸收量的交互作用十分显著,但对根锌吸收量的交互影响不显著。     

Manganese‐iron relationship in soybean grown in calcareous soils 1

Although a positive response to iron (Fe) is, usually, expected in calcareous soils; this has not been always the case; and in some instances a depressing effect has been observed. An induced micronutrient imbalance is suspected. This experiment was designed to study the effect of Fe fertilizer on the plant micronutrients. Twenty three highly calcareous soils (18–46% calcium carbonate equivalent; pH 7.7–8.4; and a wide range of extractable Fe) from southern Iran were used in an eight‐week greenhouse experiment to study the effect of Fe fertilizers on soybean [Glycine max (L.) Merr.] growth and chemical composition. The statistical design was a 23 × 3 factorial arranged in a completely randomized block with three replications. Treatments consisted of 23 soils and three levels of applied Fe (0, 10, and 20 mg Fe/kg as FeEDDHA). Uniform doses of nitrogen (N), phosphorus (P), copper (Cu), manganese (Mn), and zinc (Zn) were applied to all pots. Dry matter (DM) and micronutrients concentrations and uptakes of plant tops were determined and used as the plant responses. Application of Fe either had no significant effect on DM or even decreased it. The plant concentration and uptake of Fe increased significantly in all soils. The concentrations and uptakes of Cu and Zn did not change but those of Mn decreased significantly. The negative effect of Fe application was, therefore, attributed to the interference of Fe with Mn nutrition. The mechanism involved appears to be the restriction in Mn translocation from soil to root and/or from root to the plant tops.     

Cadmium effects on influx and transport of mineral nutrients in plant species

Solution culture experiments were conducted under controlled environmental conditions to determine the effects of cadmium(II) [Cd(II)] activity (0, 8, 14, 28, 42, and 54 μM) on influx (IN) into roots and transport (TR) from roots to shoots of zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), calcium (Ca), magnesium (Mg), phosphorus (P), and sulfur (S) in ryegrass (Lolium perenne L.), maize (Zea mays L.), white clover (Trifolium repens L.), and cabbage (Brassica oleracea var. capitata L.). Shoot and root dry matter (DM) decreased with increased external Cd, and plant species differed extensively. Ryegrass and cabbage were relatively tolerant to Cd toxicity compared to white clover and maize. Influx and TR of Cu, Zn, Fe, Mn, Ca, and Mg were lower with increasing external Cd compared to controls, and species also differed. Influx and TR of P were enhanced in each species with up to 14 μM Cd, decreased in white clover and cabbage at higher Cd levels, while in maize and ryegrass continued to increase as Cd increased. Influx and TR of S were high in white clover at 8 μM Cd and decreased as Cd increased. Influx of S was high in ryegrass, but TR of S remained relatively constant as Cd increased. Influx and TR of S did not significantly change in maize, but decreased in cabbage as Cd increased. With Cd up to 14 μM, decreases in both IN and TR of Zn, Fe, Mn, Ca, and Mg were greater in white clover than in cabbage. Sensitivity of the dicotyledonous plant species to Cd toxicity might have been associated with Cd effects on IN and TR of Fe, Mn, Ca, and Mg. However, differences in plant sensitivities to Cd toxicity between ryegrass and maize were not reflected in Cd effects on IN and TR of mineral nutrients.     

施用硒、锌、铁对玉米和大豆产量与营养

采用黄土高原低硒区典型土壤,以玉米、大豆为供试作物,研究施用硒、锌、铁对作物产量和营养品质的影响。结果表明,仅施用氮、磷、钾肥料,玉米、大豆产量无明显变化,施用适量硒、锌、铁时,玉米产量明显提高15.6%～73.8%,大豆产量提高58.9%～115.1%。施用硒、锌、铁,玉米籽粒氮含量明显增加,而磷、钾含量无明显变化,大豆籽粒氮含量无明显变化,磷、钾含量有所下降。土施或结合叶面喷施硒、锌、铁时,玉米和大豆籽粒硒、锌、铁含量明显增加,尤其是土施结合叶面喷施硒、锌、铁时效果突出。与对照比较,玉米籽粒硒增加480.4%、锌增加106.9%、铁增加126.7%; 大豆籽粒硒增加512.9%、锌增加62.2%、铁增加15.6%。综合考虑玉米、大豆产量和籽粒硒、锌、铁含量变化,锌、硒适宜土壤施用,或土施结合叶面喷施施用,叶面喷施铁可有效提高作物籽粒铁含量。     

Effect of sewage sludge and its biochar on chemical properties of two calcareous soils and maize shoot yield

Little information is available regarding the effect of sewage sludge biochar on soil properties and crop yield. Thus, our objective was to evaluate the effect of sewage sludge (S) and its biochar (B) on maize shoot yield, nutrients and heavy metals uptake in two calcareous soils. The amendments were applied at the rates of 0, 10, 20 and 40 Mg ha^?1. Moreover, NK treatment was included to compare the effects of S and B with conventional fertilization. At harvest time, plant shoots and soil samples were collected for yield, nutrients uptake and chemical analyses. The highest shoot dry matter was obtained in the S treatment. The B application in the clay loam and loam soils resulted in 5.2% increment and 17.7% decrement of shoot dry matter relative to the control, respectively. Shoot dry matter in the NK treatment was significantly higher than in the control. B application decreased Fe, Zn, Mn, Cu and Pb uptake by maize shoot. DTPA-extractable Pb in B-amended soils was lower than in control, while an inverse trend was obtained for available Fe, Zn, Mn and Cu. Biochar application at the rate of 7.3 Mg ha^?1 might be suggested for maize cultivation in clay loam soils.     

Maize Yield Response to Zinc Sources and Effectiveness of Diagnostic Indicators

Maize yield is often limited by zinc (Zn) deficiency. The objectives of this study were to (i) evaluate maize yield response to Zn applied at four different rates, (ii) evaluate the yield response and agronomic efficiency of maize to the application of a complex fertilizer, MicroEssentials SZ (12N–40P–0K–10S–1Zn), compared to different rates of monoammonium phosphate (MAP) + ammonium sulfate (AS) + zinc sulfate (ZnSO4), and (iii) evaluate the association between tissue Zn concentration and soil-test Zn with the maize response to Zn fertilizer. Eleven experiments were carried out during the 2010, 2011, and 2012 growing seasons throughout eight states in the USA. Treatments consisted of four Zn rates of a physical blend of MAP + AS + ZnSO₄ (0, 2.24, 4.48, 6.72, and 11.2 kg/ha Zn) and MicroEssentials SZ at a Zn rate of 2.24 kg/ha Zn. Nitrogen, phosphorus (P), and sulfur (S) rates were balanced across treatments (40 kg/ha P, 22 kg/ha S) and fertilizers were broadcast and incorporated immediately prior to planting. Treatment and location main effects were significant (P < 0.001) on corn yields, whereas the interaction treatment × location was not (P = 0.33). Maize responded positively to Zn fertilization; average yields across locations increased from 10,540 kg ha^?1 without Zn to 11,530 kg ha^?1 with 11.21 kg Zn ha^?1 applied as a physical blend. The yield response and Zn agronomic efficiency of maize with the application of the complex fertilizer at a rate of 2.24 kg Zn ha^?1 averaged 1004 kg ha^?1 and 448 kg maize kg Zn^?1, respectively, significantly higher (P < 0.1) than the yield response and Zn agronomic efficiency with the application of a physical blend with the same Zn rate, which averaged 293 kg ha^?1 and 131 kg maize kg Zn^?1, respectively. The Zn concentration in plant tissue of unfertilized plots varied greatly and was not related to the maize response to Zn fertilizer (r = 0.01; P = 0.98). With respect to soil Zn, a negative but nonsignificant relationship was found between maize response to Zn fertilizer and soil-test Zn (r = ?0.51; P = 0.16).     

Effect of indigenous arbuscular mycorrhizal fungi combined with manure on the change in concentration some mineral elements in cotton (Gossypium hirsutum L.)

A pot experiment was conducted to evaluate the effect of indigenous arbuscular mycorrhizal fungi (AMF) and the synergy of indigenous AMF and sheep manure (SM) on potassium (K), calcium (Ca) and some micronutrient concentrations in cotton plant. Indigenous AMF were a mixture of Glomus viscosum, Glomus mosseae and Glomus intraradices initially isolated from a cotton field. Cotton was grown for 12 weeks and the elements of shoot were determined at three stages of plant growth. Inoculated cotton plants with AMF had higher concentrations of K, Ca, manganese (Mn), iron (Fe), copper (Cu) and zinc (Zn) than non-mycorrhizal plants. Shoot concentrations of these elements increased significantly when SM was added to mycorrhizal plants. Maximum plant micronutrient uptake was found in the treatment of AMF inoculation with SM. Mn, Fe, Cu and Zn uptake increased significantly by 457%, 282%, 272% and 295%, respectively, over control. Indigenous AMF combined with SM resulted in better plant growth and micronutrient uptake.