Crop sequences and fertilization affect soil vital enzyme activities

This experiment was conducted in split plots based on randomized complete block design with three replications. Three crop sequences: (R1): chickpea, sunflower, wheat, and canola; (R2): green manure, chickpea, green manure, wheat, green manure, and canola; (R3): canola, wheat, and canola were used as main plots. Sub plots consisted of six methods of fertilization: (N1): farmyard manure; (N2): compost; (N3): chemical fertilizers; (N4): farmyard manure + compost; (N5): farmyard manure + compost + chemical fertilizers; and (N6): control. Results showed that the enzyme activities were higher in the N4 treatment. The highest amount of acid phosphatase, protease, dehydrogenase activity, and grain yield was observed in R2 sequence. The highest urease activity (58.6 µg g^?1 h^?1) was obtained in R2N4 treatment. In R2N4 treatment using in-farm inputs, a non chemical fertilizer system can be carried out to improve soil biological activity.     

Response of nectarines to organic fertilization under the rain-fed ecosystem of Northwest Himalayas

The comparative efficacy of organic sources on cropping behavior, nutrient dynamics, physico-chemical and biological properties of soil and fruit quality under rain-fed agroecosystem on “Silver King” nectarines was investigated. Bio-organic nutrients, namely vermi-compost (VC), biofertilizer (BF), farmyard manure (FYM), compost (comp), vermiwash (VW) and cow urine (CU) were evaluated in 11 different treatment combinations. The treatment application of VC at 25 kg tree^?1, BF at 40 g tree^?1, FYM at 30 kg tree^?1, comp at 15 kg tree^?1, VW_1:10 and CU_1:10 significantly improved plant growth and fruit quality characteristics of nectarine trees. This superior combination also enhanced physico-chemical and biological properties of the rhizosphere soil when compared to control as conventional chemical fertilizer application nitrogen, phosphorus and potassium (NPK) fertilizers. Different treatments of bio-organic sources changed pH of the soil to neutral. Available macronutrient contents of soil (viz. N, P, and K) increased by 57.8%, 27.7%, and 16.4%, respectively. Microbial biomass of soil fungi, total bacteria, actinobacteria, and arbuscular mycorrhizal fungi improved 66.0%, 73.8%, 133.3% and 350.0%, respectively, over control. Considerably, a higher amount of leaf macronutrients, N (3.53%), P (0.23%), and K (3.2%), was also recorded over control.     

不同施肥和耕作制度下土壤微生物多样性研究进展

本文主要介绍了运用Biolog GN、磷脂脂肪酸(PLFA)、核酸分析法进行土壤微生物群落分析的优缺点,综述了施肥、耕作两种农业措施对土壤微生物多样性影响的研究进展。指出不同施肥处理对微生物影响效果不同,合理施用有机肥有利于维持土壤微生物的多样性及活性;由于受其他环境因素(如土壤类型、农作制度、残茬量等)的影响,不同耕作措施对土壤微生物多样性影响有差异,但是大多试验结果显示免耕、少耕能增加微生物多样性和生物量,保持系统的稳定性。文章还指出了目前研究中存在的问题,并对今后的研究方向做了展望。     

Soil respiration from winter wheat-based cropping systems in the US Southern Great Plains as influenced by tillage managements

This study compared soil respiration (SR) fluxes from winter wheat-based cropping systems in the US Southern Great Plains (SGP) under reduced and conventional tillage. The study consisted of four sets of paired paddocks assigned to conventional or reduced tillage with a four-year crop rotation applied over time. During the 2016–2017 study year, four sets of paired paddocks were planted to: winter wheat managed for grazing, dual-purpose (grazing and grain production), and grain-only systems of production, and winter canola. Heterotrophic SR fluxes were measured using a plant and root exclusion method on eight permanently deployed PVC cores per paddock. Fluxes from the cores were measured manually using a closed chamber connected to an infrared gas analyser on 12–13 dates during the winter wheat growing season (October through May). There were strong seasonal patterns of SR flux, with lower rates during dry and cold periods, and higher rates during warm and wet periods. Large rainfall induced pulses of SR flux were observed for both tillage systems. There was no consistent large-scale difference in SR flux between tillage treatments applied to paired paddocks. Results from this study indicate SR fluxes from winter wheat-based cropping systems are controlled more by soil environmental conditions than form of tillage.     

Combining conservation tillage with nitrogen fertilization promotes maize straw decomposition by regulating soil microbial community and enzyme activities

Straw return can effectively improve farmland soil microenvironment and fertility. However, excessive straw in the topsoil adversely affects seed germination and crop growth. At present, the characteristics and key driving factors of straw decomposition in dry farmlands are unclear. Based on the interactions between tillage practices including zero tillage (ZT), chisel tillage (CT), and plow tillage (PT) and nitrogen (N) fertilization, i.e., low N (N1, 180 kg ha^-1), normal N (N2, 240 kg ha^-1), and high N (N3, 300 kg ha^-1), quantitative polymerase chain reaction technology and an enzymatic detection kit were used to investigate the effects of key straw C-degrading enzyme activities and microbial abundance in soil on maize straw decomposition during the growth period of winter wheat in the winter wheat/summer maize double cropping system in a dry farmland of the Loess Plateau, China. Between 2018 and 2020, ZT and CT significantly increased winter wheat yield (by 10.94% and 12.79%, respectively) and straw decomposition velocity (by 20% and 26.67%, respectively), compared with PT. Compared to N1 and N3, N2 significantly increased wheat yield (by 4.65% and 5.31%, respectively) and straw decomposition velocity (by 26.33% and 13.21%, respectively). The partial least squares pathway modelling showed significant positive direct effects of soil moisture, NO-₃, NH⁺₄, total N, bacteria, and cellulase, laccase, and xylanase activities on straw decomposition, while soil pH, fungi, and Actinomycetes had significant negative direct effects. Overall, conservation tillage (ZT and CT) combined with N2 was beneficial for straw decomposition in the drylands of the Loess Plateau and improved straw resource utilization and basic soil fertility. The results of the study clarified the key drivers of straw decomposition in dry farmlands and provided new ideas for developing updated soil management practices and adaptive N application strategies to promote the resource utilization of straw and achieve the goals of carbon peaking and carbon neutrality.     

长期施肥对黑土酶活性和微生物呼吸的影响

以农业部哈尔滨黑土生态环境重点野外科学观测试验站28年长期定位试验为平台,研究了长期施用不同肥料对黑土酶活性及微生物呼吸强度的影响。结果表明,长期不同施肥处理后黑土过氧化氢酶、转化酶和脲酶的活性均产生较大的差异,有机无机肥料配合施用处理的土壤中脲酶和转化酶活性显著地高于单施化肥、有机肥和不施肥处理,施肥对土壤过氧化氢酶具有一定的抑制作用。相同施肥条件下0~20 cm土层土壤过氧化氢酶、转化酶和脲酶的活性均高于20~40 cm土层酶活性。此外,施肥对土壤真菌、细菌的呼吸都有一定的抑制作用。     

长期施肥与耕作方式对土壤酶活性的影响

通过长期田间试验,研究了白浆土耕种18年后施肥与耕作方式对土壤酶活性的影响。结果表明:无论轮作还是连作施肥,土壤脲酶、蔗糖酶、中性磷酸酶活性均有不同程度提高,施用厩肥尤为明显,OM处理的脲酶活性最大,与CF、CK处理间差异显著,轮作的土壤脲酶活性和中性磷酸酶活性均高于连作;深松处理配施化肥可以提高白浆土的蔗糖酶和脲酶活性,且轮作影响大于连作。     

Nitrous oxide emissions in a winter wheat – summer maize double cropping system under different tillage and fertilizer management

An accurate estimation of nitrous oxide (N₂O) emission from 110 million ha of upland in China is essential for the adoption of effective mitigation strategies. In this study, the effects of different tillage practices combined with nitrogen (N) fertilizer applications on N₂O emission in soils were considered for a winter wheat (Triticum aestivum L.) – summer maize (Zea mays L.) double cropping system. Treatments included conventional tillage plus urea in split application (CTF1), conventional tillage with urea in a single application (CTF2), no‐tillage with straw retained plus reduced urea in a split application (NTSF1) and no‐tillage with manure plus reduced urea in a split application (NTMF1). The amounts of N input in each treatment were 285 and 225 kg N/ha for wheat and maize, respectively. Both NTSF1 and NTMF1 were found to reduce chemical N fertilizer rates by 33.3% (wheat) and 20% (maize), respectively, compared to CTF1 and CTF2. N₂O emissions varied between 3.2 (NTSF1) and 9.9 (CTF2) kg N₂O‐N/ha during the wheat season and between 7.6 (NTFS1) and 14.0 (NTMF1) kg N₂O‐N/ha during the maize season. The yield‐based emission factors ranged from 21.9 (NTSF1) to 60.9 (CTF2) g N₂O‐N/kg N for wheat and 92.5 (NTSF1) to 157.4 (NTMF1) g N₂O‐N/kg N for maize. No significant effect of the treatments on crop yield was found. In addition to reducing production costs involved in land preparation, NTSF1 was shown to decrease chemical fertilizer input and mitigate N₂O emissions while sustaining crop yield.     

Response of organic matter to reduced tillage and animal manure in a temperate loamy soil

The impacts of tillage and organic fertilization on soil organic matter (SOM) are highly variable and still unpredictable, and their interactions need to be investigated under various soil, climate and cropping system conditions. Our work examined the effect of reduced tillage and animal manure on SOM stocks and quality in the 0–40 cm layer of a loamy soil under mixed cropping system and humid temperate climate. The soil organic carbon (SOC) and N stocks, particulate organic matter (POM), and C and N mineralization potential (301 days at 15 °C) were measured in a 8‐yr‐old split‐plot field trial, including three tillage treatments [mouldboard ploughing (MP), shallow tillage (ST), no tillage (NT)] and two fertilization treatments [mineral (M), poultry manure 2.2 t/ha/yr C (O)]. No statistically significant interactive effects of tillage and fertilization were measured except on C mineralization. NT and ST showed greater SOC stocks (41.2 and 39.7 t/ha C) than MP (37.1 t/ha C) in the 0–15 cm increment, while no statistical differences were observed at a greater depth. N stocks exhibited similar distribution patterns with regard to tillage effect. Animal manure, applied at a rate representative of typical field application rates, had a smaller impact on SOC and N stocks than tillage. The mean SOC and N stocks were higher under O than M, but the differences were statistically significant only in the 0–5 cm increment. MP showed lower C‐POM stocks than NT and ST in the 0–5 cm increment, whereas greater C‐POM stocks were measured under MP than under NT or under ST in the 20–25 cm increment. Organic fertilization had no impact on C‐POM or N‐POM stocks. In the 0–25 cm increment, NT showed a lower C and N mineralization potential than MP. Our work shows that the sensitivity of SOM to reduced tillage for the whole soil profile can be relatively small in a loamy soil, under humid‐temperate climate. However, POM was particularly sensitive to the differential effects of tillage practices with depth, and indicative of differentiation in total SOM distribution in the soil profile.     

Soil tillage and crop productivity on a Vertisol in Ethiopian highlands

Soil quality deterioration and consequent reduced productivity characterize the Vertisols in the highlands of Ethiopia. The problem is exacerbated by lack of appropriate land preparation alternatives for the major crops in the area. A field experiment was carried out for 6 years (1998–2003) at Caffee Doonsa in the central highlands of Ethiopia to evaluate alternative land preparation methods on the performance of wheat (Triticum durum Desf.), lentil (Lens culinaries Medik L) and tef (Eragrostis tef L) grown in rotation. Four methods of land preparation (broad bed and furrow, green manure, ridge and furrow and reduced tillage) were arranged in a randomized complete block design with three replications on permanent plots of 22 m by 6 m. Broad bed and furrow significantly increased the grain yield of lentils by 59% (from 1029 to 1632 kg ha⁻¹) as compared to the control. On the other hand, reduced tillage resulted in the highest grain yield of wheat (1862 kg ha⁻¹) and tef (1378 kg ha⁻¹) as compared to 1698 kg ha⁻¹ of wheat and 1274 kg ha⁻¹ of tef for the control although the increase was not statistically significant. A gross margin analysis showed that BBF is the most profitable option for lentil with 65% increase in total gross margin. On the other hand, RT resulted in 11 and 8% increase in gross margin of wheat and tef, respectively as compared to the control. Based on the agronomic and economic performances best combinations of crop and land preparation method were: lentil sown on broad bed and furrow, and wheat and tef sown after reduced tillage.     

不同耕作方式下旱作玉米田土壤呼吸及其影响因素

为揭示不同耕作方式对旱作玉米田土壤呼吸的影响,对比研究深松耕、免耕、旋耕和翻耕4种耕作方式下土壤呼吸速率的动态变化及其与土壤水分、温度、有机质、全氮、pH值等的关系。结果表明,夏玉米生长季,4种耕作方式下土壤呼吸速率随生育时期均呈先增加后降低的趋势,平均土壤呼吸速率为深松耕>翻耕>旋耕>免耕;播种前至拔节期土壤温度为翻耕>深松耕>旋耕>免耕,抽雄期至成熟收获期为免耕>旋耕>深松耕>翻耕;各耕作方式下0～20cm层土壤有机质、全氮均逐渐增加,与免耕比较,翻耕有机质和全氮均降低;生育前期土壤pH值波动明显,抽雄期后趋于平缓,土壤pH值平均值为翻耕>旋耕>免耕>深松耕。各影响因素与土壤呼吸速率相关分析表明,深松耕和翻耕土壤水分、温度与土壤呼吸速率呈显著或极显著正相关;有机质与土壤呼吸速率呈负相关,且与深松耕措施下土壤呼吸速率呈显著负相关;除免耕外,其他耕作方式下土壤全氮、pH值与土壤呼吸呈负相关。该研究可为补充完善土壤呼吸排放机理、评估区域碳收支平衡及制定科学有效的土壤碳调控管理措施提供依据。     

不同耕作方式对绿洲区夏玉米农田土壤呼吸及酶活性的影响

[目的]研究民勤荒漠绿洲区免耕(Tn)、少耕(Tm)、深松(Ts)和秋翻(Tf)4种耕作方式下土壤呼吸速率的动态变化及其与土壤酶活性的关系,为制定科学有效的土壤碳调控管理措施提供依据。[方法]在2a的田间定位试验基础上,利用LI-8100土壤碳通量测量系统测定不同生育时期(苗期、抽穗期和成熟期)玉米田土壤呼吸速率动态变化,同时取0—20cm土样测定土壤酶活性和理化性质。[结果](1)民勤荒漠绿洲区土壤呼吸具有典型的日动态变化,4种耕作措施土壤呼吸速率日变化在玉米整个生育期呈单峰曲线变化,土壤呼吸速率依次为:TfTmTsTn,有机碳含量与土壤呼吸速率呈显著正相关(p0.05),说明在民勤荒漠绿洲区,传统耕作明显加快了玉米农田土壤碳的释放。(2)土壤脲酶、蔗糖酶、蛋白酶、过氧化氢酶和β-葡萄糖苷酶活性与土壤呼吸有较好相关性(p0.05),其中与过氧化氢酶、脲酶、蔗糖酶活性达到极显著水平(p0.01);pH值、速效钾、有机碳与脲酶、蔗糖酶、β-葡糖糖苷酶活性达到极显著水平(p0.01)。[结论]耕作方式可以通过改变荒漠绿洲区土壤理化性质、激发酶活性从而使土壤呼吸速率发生不同程度的改变,影响玉米田CO2的释放。     

Soil microbial activity and crop sustainability in a long-term experiment with three soil-tillage and two crop-rotation systems

Reduction in soil disturbance can stimulate soil microbial biomass and improve its metabolic efficiency, resulting in better soil quality, which in turn, can increase crop productivity. In this study we evaluated microbial biomass of C (MB-C) by the fumigation-extraction (FE) or fumigation-incubation (FI) method; microbial biomass of N (MB-N); basal respiration (BR) induced or not with sucrose; metabolic quotient (obtained by the ratio BR/MB-C) induced (qCO₂(S)), or not with sucrose (qCO₂); and crop productivity in a 14-year experiment in the state of Paraná, southern Brazil. The experiment consisted of three soil-tillage systems [no-tillage (NT), conventional tillage (CT) and no-tillage using a field cultivator every 3 years (FC)] and two cropping systems [a soybean–wheat-crop sequence (CS), and a soybean–wheat–white lupin–maize–black oat–radish crop rotation (CR)]. There were six samplings in the 14th year, starting at the end of the winter crop (wheat in the CS and lupin in the CR plots) and finishing at full flowering of the summer crop (soybean in the CS and maize in the CR). Differences in microbiological parameters were greater than those detected in the total C (TCS) and total N (TNS) contents of the soil organic matter (SOM). Major differences were attributed to tillage, and on average NT was higher than the CT in the following parameters: TCS (19%), TNS (21%), MB-C evaluated by FE (74%) and FI (107%), and MB-N (142%). The sensibility of the microbial community and processes to soil disturbance in the tropics was highlighted, as even a moderate soil disturbance every 3 years (FC) affected microbial parameters but not SOM. The BR was the parameter that most promptly responded to soil disturbance, and strong differences were perceived by the ratio of qCO₂ evaluated with samples induced and non-induced with sucrose. At plowing, the qCO₂(S):qCO₂ was five times higher under CT, indicating a C-starving low-effective microbial population in the C-usage. In general, crop rotation had no effect on microbial parameters or SOM. Grain yield was affected by tillage and N was identified as a limiting nutrient. Linear regressions between grain yields and microbial parameters showed that soybean was benefited from improvements in the microbial biomass and metabolic efficiency, but with no significant effects observed for the maize crop. The results also indicate that the turnover of C and N in microbial communities in tropical soils is rapid, reinforcing the need to minimize soil disturbance and to balance inputs of N and C.     

保护性耕作对旱作农田耕层土壤肥力及酶活性的影响

通过田间定位试验,研究了不同耕作方式对黄土高原西部旱农区耕层土壤肥力和酶活性的影响。结果表明,秸秆还田可以显著提高 0—5和5—10 cm土层有机质、全氮、全磷、全钾、铵态氮、速效磷、速效钾和3种水解酶活性; 10—30 cm 土层仅提高了有机质、全钾和速效钾含量,对其余各养分含量和水解酶活性并无明显影响。免耕降低了0—5、5—10和10—30 cm土层硝态氮含量,但对过氧化氢酶活性有明显促进作用。相关分析表明,土壤有机质、养分和碱性磷酸酶、蔗糖酶活性之间呈极显著相关关系。进一步应用主成分分析表明,土壤有机质、养分和水解酶活性共同反映着黄土高原雨养农区土壤肥力水平的高低。     

耕作管理措施对残茬覆盖及其分解的影响

The effects of tillage methods on percent surface residue cover remaining and decomposition rates of crop residues were evaluated in this study. The line transect method was used to measure residue cover percentage on continuous corn (Zea mays L.) plots under no tillage (NT), conventional tillage (CT), chisel plow (CH), and disk tillage (DT). Samples of rye (Secale cereale L.) and hairy vetch (Vicia villosa Roth) were used for residue decomposition study. Results showed that the percentage of residue cover remaining was significantly higher for NT than for CH and DT and that for CT was the lowest (< 10%). For the same tillage system, the percent residue cover remaining was significantly higher in the higher fertilizer N rate treatments relative to the lower fertilizer N treatments. Weight losses of rye and vetch residues followed a similar pattern under CT and DT, and they were significantly faster in CT and DT than in NT system. Also, the amounts of residue N remaining during the first 16 weeks were always higher under NT than under CT and DT.     

Soil tillage and cover crop on soil CO2 emissions from sugarcane fields

Soil tillage is an agricultural practice that directly affects the global carbon cycle. Our study sought to assess the implications of adopting sunn hemp cover crops with different tillage practices on CO₂ emissions for two soil types (clayey and sandy soil) cultivated with sugarcane in Brazil. The experimental design was a split‐plot with randomized blocks, with the main plots being with cover crop or fallow and sub‐plots being under conventional or minimum tillage. Our results indicate that during the first 50 days after soil tillage, the variation in soil CO₂ emissions was stimulated by cover crop and soil tillage, while after that, it became dominated by the root respiration of sugarcane plants. We also found that over the first 97 days after the tillage, the clayey soil showed differences between minimum tillage with cover crop and fallow. Conversely, for sandy soil over the first 50 days following, there were differences between the tillage systems under cover cropping. Emissions from sugarcane rows were found to be greater than those from inter‐row positions. We concluded that soils under different textural classes had distinct patterns in terms of soil CO₂ emissions. The correct quantification of CO₂ emissions during the sugarcane renovation period should prioritize having a short assessment period (~50 days after soil tillage) as well as including measurements at row and inter‐row positions.     

蔬菜废弃物堆肥对设施蔬菜产量和土壤生物特性的影响

采用盆栽试验研究了蔬菜废弃物堆肥对小白菜的增产效果、土壤养分含量、土壤微生物量和酶活性的影响。结果表明,蔬菜废弃物堆肥能够显著提高盆栽小白菜的产量和品质,其中30 t/hm2的高量蔬菜废弃物堆肥将产量提高了66.26%,将品质指标Vc、可溶性糖和可溶性蛋白含量显著提高了35.64%、183.36%和39.42%。蔬菜废弃物堆肥能够显著提高土壤质量,有机质、总氮、碱解氮、有效磷、速效钾含量和土壤微生物量碳、氮,以及土壤淀粉酶、脲酶、磷酸酶、脱氢酶活性的土壤质量指标。与牛粪相比较,高用量的蔬菜废弃物堆肥处理在小白菜产量、可溶性蛋白含量、土壤碱解氮、有效磷、速效钾含量、磷酸酶活性上显著高于牛粪;而在可溶性糖含量、土壤有机质含量、土壤微生物量、淀粉酶、脱氢酶活性上显著低于牛粪。综合而言,蔬菜废弃物堆肥能够提高土壤质量,增加蔬菜产量和品质;在蔬菜产量方面,蔬菜废弃物堆肥优于牛粪,在蔬菜品质和土壤质量方面,蔬菜废弃物堆肥与牛粪相当。     

Soil microbial community response to controlled-release urea fertilizer under zero tillage and conventional tillage

Soil microorganisms mediate many important biological processes for sustainable agriculture. The effect of a polymer-coated controlled-release urea (CRU, ESN^®) on soil microbial communities was studied at six sites across western Canada from 2004 to 2006. Fertilizer treatments were CRU, urea and an unfertilized control. Timing of fertilizer application (fall vs. spring) was studied in 9 of the 18 site-years (combinations of sites and years). Wheat (Triticum aestivum L.), canola (Brassica napus L.) and barley (Hordeum vulgare L.) were grown in rotation at five sites, and silage corn (Zea mays L.) was grown in all 3 years at one site, under conventional tillage (CT) or zero tillage (ZT). The fertilizers were side-banded at 50–60 kg N ha⁻¹ for wheat, barley and canola, and broadcast at 150 kg N ha⁻¹ for corn. Microbial biomass C (MBC) and bacterial functional diversity and community-level physiological profiles (CLPPs) were determined at about the flowering stage of each crop. In situ CO₂ evolution (soil respiration) was measured, and microbial metabolic quotient (qCO₂) determined, at one site in 2 years. In the rhizosphere, fertilizer effects on MBC and functional diversity were observed in 1 and 5 of 18 site-years, respectively; and in bulk soil in 4 site-years each. These effects were usually positive relative to the control. CRU increased MBC or functional diversity more than urea in 3 site-years, but the opposite was observed in 1 site-year. Time of fertilizer application affected MBC in 1, and functional diversity in 2, of 9 site-years in the rhizosphere, and no effects were observed in bulk soil. Fall-applied fertilizer increased MBC more than spring-applied fertilizer, but the opposite was observed for functional diversity. Tillage affected MBC and functional diversity in 4 and 5 of 18 site-years, respectively, in the rhizosphere, and in 3 and 4 site-years in bulk soil. Tillage effects were usually in favour of ZT. There were no treatment effects on CO₂ evolution, but an interactive effect of fertilizer and tillage on qCO₂ was observed in 1 year when qCO₂ in the control treatment was greater than that in either fertilizer treatment under CT, but urea increased qCO₂ relative to the control under ZT. Shifts in CLPPs were sometimes observed where the treatment effects described above were not significant. Notwithstanding the limitations of culture-dependent CLPPs, most fertilizer effects on soil microbiological properties were not statistically significant. Therefore, these fertilizers probably did not adversely affect most soil biological processes.     

转变耕作方式对长期旋免耕农田土壤有机碳库的影响

土壤深松是解决长期旋免耕农田耕层浅薄化、亚表层（>15～30 cm）容重增加等问题的有效方法之一,而将长期旋免耕农田进行深松必然导致农业生态系统中土壤有机碳（soil organic carbon,SOC）及碳固定速率的变化。因此,为对比将长期旋免耕转变为深松前后农田土壤有机碳库变化,该研究利用连续12a 的旋耕和免耕长期定位试验以及在此基础上连续6 a旋耕-深松和免耕-深松定位试验,对比了转变耕作方式对农田土壤0～30 cm有机碳含量、周年累积速率及其固碳量的影响。研究结果表明,经过连续12 a的旋耕和免耕处理（2002－2014）,2014年免耕处理土壤0～30 cm有机碳储量比试验初期（2002年）提高38%,旋耕处理降低了30%,而对照常规处理无显著差异。免耕处理土壤0～30 cm有机碳储量比旋耕处理高约2.6倍（2014年）。长期免耕显著提高了土壤0～30 cm的有机碳含量,2002~2014年其土壤0～30 cm固碳量为16.69 t/hm2,但长期旋耕导致土壤0～30 cm SOC含量显著降低,表现为土壤有机碳的净损耗,年损耗速率为?0.75 t/hm2。而长期旋耕后进行深松（旋耕-深松处理）6年其土壤0～30 cm的有机碳含量较原旋耕处理提高32%～67%,且显著提高了土壤固碳量及周年累积速率;免耕-深松土壤0～30 cm的有机碳周年累积速率较免耕处理下降了42%。长期旋耕造成有机碳水平下降的条件下,将旋耕处理转变为深松处理在短期内更有利于促进土壤有机碳的积累,而将长期免耕处理转变为深松措施,降低了土壤有机碳的累积速率和固碳量。     

Soil microbial biomass and activity response to repeated drying-rewetting cycles along a soil fertility gradient modified by long-term fertilization management practices

The effects of repeated drying-rewetting (DRW) cycles on the microbial biomass and activity in soils taken from long-term field experiment plots with different fertilization (FERT) management practice histories were studied. We investigated the hypothesis that soil response to DRW cycles differs with soil fertility gradient modified by FERT management practices. The soils were incubated for 51 days, after exposure to either nine or three DRW cycles, or remaining at constant moisture content (CMC) at field capacity. We found that both DRW and FERT significantly affected soil properties including NH₄-N, NO₃-N, dissolved organic C (DOC), microbial biomass C (Cmic), basal soil respiration rate (BSR), urease activity (URE) and dehydrogenase activity (DHD). Except for NH₄-N and BSR, variation in the properties was largely explained by FERT, followed by DRW, and then their interaction. Irrespective of the soils' FERT treatment, repeated DRW cycles significantly raised the DOC and Cmic levels compared with CMC, and the DRW cycles also resulted in a significant decline in BSR and URE and increase in DHD, probably because the organisms were better-adapted to the drying and rewetting stresses. The variations in soil biological properties caused by DRW cycles showed a significantly negative relationship with the soil organic C content measured prior to the start of the DRW experiments, suggesting that soils with higher fertility are better able to maintain their original biological functions (i.e., have a higher functional stability) in response to DRW cycles.