Effects of biochar and litter on carbon and nitrogen mineralization and soil microbial community structure in a China fir plantation

To investigate the effects of biochar addition(1 or 3%)to the soil of a China fir plantation with or without litter,we conducted a 90-day incubation experiment.We also studied the C and N dynamics and the microbial community structure of the soil.In soil without litter,the application of biochar at a rate of 3%significantly decreased CO2 emissions,while addition of 1%biochar had no effect.Biochar application did not affect the net N mineralization rate but significantly reduced the NH4?concentration after 90 days.In litter-enriched soil,biochar application had no significant effect on total CO2 emissions;however,application of 3%biochar significantly reduced the net N mineralization rate.Biochar application to soil with or without litter immediately reduced the dissolved organic carbon(DOC)concentration independent of the application rate,which was primarily due to sorption of DOC by the biochar.Phospholipid fatty acid analysis demonstrated that both concentrations of added biochar to soil(with or without litter)altered the soil microbial community structure at the end of incubation,although the effect of biochar was not as strong as the effect of time or litter application.The effect of biochar addition alone on microbial community structure was inconsistent over time.Litter added to soil significantly increased fungi and reduced Gram-positive bacteria.In the presence of litter,biochar applied at both 1%and 3%significantly increased(p<0.05)the proportion of actinomycete only at day 90.Our results indicate biochar as a potentially effective measure for C sequestration in the test soil of a China fir plantation,even in the presence of litter.     

间伐对侧柏人工林土壤微生物生物量碳、氮的影响

以徐州侧柏人工林为对象,研究了在弱度间伐（LIT,20％）、轻中度间伐（MIT1,40％）、中度间伐（MIT2,60％）和强度间伐（HIT,80％）下,林下土壤微生物生物量碳、氮在土壤表层（0 ～ 10 cm）和亚表层（10～20 cm）的变化.结果表明：不同间伐强度下,微生物生物量碳、氮含量均随着土层加深而减少.随着间伐强度增加,微生物生物量碳、氮总体变化趋势为先增加后降低,其中微生物生物量碳表现为MITl＞ LIT＞ HIT＞ MIT2,均与对照CK有显著差异（P＜0.05）;微生物生物量氮表现为MIT2＞ MITl＞ LIT＞ HIT,各处理间均表现出显著差异（P＜0.05）.间伐也使土壤微生物熵增加,尤以MIT2达到最大（2.63％）.研究表明,侧柏人工林经过2 a间伐,土壤微生物生物量碳、氮含量增加,在一定程度上使土壤碳库和氮库短期内出现不稳定趋势.     

沼液施肥对杨树林地土壤微生物量碳氮的影响

沼液是沼气发酵过后的液体残留物,是一种优质的有机肥料。研究了不同沼液施肥对杨树林地土壤微生物量碳氮的影响。结果表明:施用沼液可以提高土壤微生物量碳、氮的含量,微生物量碳、氮含量增加范围分别为4.29%~61.62%和6.08%~76.10%,土壤微生物量碳氮含量随沼液施用量增加逐渐提高。土壤微生物量碳氮呈正相关。土壤微生物量碳氮比随沼液施用量增加逐渐下降,其变化范围为9.92~10.81,土壤微生物量碳氮比与微生物量碳、氮含量负相关。     

Nitrogen additions inhibit nitrification in acidic soils in a subtropical pine plantation: effects of soil pH and compositional shifts in microbial groups

Plantation forests play a pivotal role in carbon sequestration in terrestrial ecosystems, but enhanced nitrogen(N) deposition in these forests may affect plantation productivity by altering soil N cycling. Hence,understanding how simulated N deposition affects the rate and direction of soil N transformation is critically important in predicting responses of plantation productivity in the context of N loading. This study reports the effects of N addition rate(0, 40, and 120 kg N ha^-1 a^-1) and form(NH_4Cl vs. NaNO_3) on net N mineralization and nitrification estimated by in situ soil core incubation and on-soil microbial biomass determined by the phospholipid fatty acid(PLFA) method in a subtropical pine plantation. N additions had no influences on net N mineralization throughout the year. Net nitrification rate was significantly reduced by additions of both NH_4Cl(71.5) and NaNO_3(47.1%) during the active growing season, with the stronger inhibitory effect at high N rates. Soil pH was markedly decreased by 0.16 units by NH_4Cl additions. N inputs significantly decreased the ratio of fungal-to-bacterial PLFAs on average by 0.28(49.1%) in November. Under NH_4Cl additions, nitrification was positively related with fungal biomass and soil pH. Under NaNO_3 additions,nitrification was positively related with all microbial groups except for bacterial biomass. We conclude that simulated N deposition inhibited net nitrification in the acidic soils of a subtropical plantation forest in China,primarily due to accelerated soil acidification and compositional shifts in microbial functional groups. These findings may facilitate a better mechanistic understanding of soil N cycling in the context of N loading.     

杉木火力楠混交林与杉木纯林土壤碳氮库研究

通过实地调查取样和室内C、N元素分析仪的测定,比较了杉木纯林与杉木火力楠混交林的土壤碳库及垂直分布差异,结果显示:混交林的土壤有机碳含量比纯林高,其有机碳贮量比杉木纯林大17.57%,主要差异在枯枝落叶层,分别为3.620 t.hm-2和12.610 t.hm-2。有机碳富集指数20~40 cm差异最大,混交林富集指数是纯林的1.18倍。混交林土壤有机碳贮量(79.460 t.hm-2)大于杉木纯林(67.583 t.hm-2),且均以表层(0~20 cm)碳贮量为主。混交林的全氮含量高于纯林,C/N则低于纯林。这些差异主要是由不同林分凋落物数量和性质上的差异引起的。杉木和火力楠混交林比杉木纯林更有利于碳的贮存,人工造林应多发展混交林。     

杉木人工林土壤养分变化规律

该文从不同立地条件、不同发育阶段、不同密度管理和不同轮伐期4个方面具体阐述了杉木人工林土壤养分变化规律。指出:立地不同土壤肥力不同,立地条件越好,土壤养分含量越高;林分发育阶段不同土壤养分含量不同,幼龄林阶段,养分含量越高,中龄林时最低,成熟林时又有所回升;杉木林通过合理间伐,适当降低林分密度,可保持和提高土壤肥力;适当延长轮伐期有利于杉木林的养分归还。在对各种研究结果与结论作简要概括的同时,对当前研究工作存在的问题及今后研究的重点进行了讨论。旨在为今后的研究提供阶梯,以有助于各项研究工作的展开。     

杉木生态系统生物量与固碳能力的分析与评价

杉木(Cunninghamia lanceolata)是我国特有的优良速生针叶树种,分布地域广阔,在碳循环及维护生态系统平衡等方面发挥着非常重要的作用。本文通过分析大量文献,讨论了立地条件、分布区域和经营方式等因素对杉木林生态系统生物量和生产力的影响。根据文献资料对杉木林生态系统生物量和固碳能力进行了初步估测。结果表明:①中国杉木林生态系统平均生物量约为36.516 t.hm-2,平均生产力约为8.412 t.hm-2.a-1。杉木林生产力的最大值在杉木中心分布区的中亚热带,尤以中亚热带南部亚地带的最高,其生产力平均达13.50 t.hm-2.a-1;中亚热带北部亚地带平均为11.95 t.hm-2.a-1;南亚热带和北亚热带分别是8.83 t.hm-2.a-1和5.54 t.hm-2.a-1;北热带地区杉木林的生物生产力最低,平均为5.02t.hm-2.a-1。②1994年以前的统计数据,中国杉木林生态系统的总植物碳储量为:幼龄林9.98×106t,中龄林31.61×106t,近熟林11.73×106t,成熟林7.50×106t,过熟林2.87×106t,总计为63.69×106t。③目前,中国杉木林面积达1 239.1×104hm2,蓄积量为47 357.33×104m3,换算成生物量约为18 938.20×104t,总固碳量约为5 211.65×104t.a-1。目前,杉木林生态系统的碳储量的估算没有包括土壤以及凋落物层的碳含量,因此,所估算的杉木林固碳能力和总的碳储量可能偏低。     

不同营林方式对杉木幼林生长及土壤微生物数量的影响

对不同营林方式的杉木幼林生长量及土壤细菌、真菌、放线菌的数量进行了研究。结果表明:不炼山、定植2年生容器苗、造林后免抚育的生态型模式,相比炼山、定植1年生裸根苗、造林后抚育三年的传统型模式,杉木幼株生长量没有显著性差异;生态型营林方式减少了对微生物的破坏,且有利于微生物数量的恢复,建议在生产中推广生态型营林方式。     

短周期杉木人工林不同林龄土壤肥力综合评价

为探讨不同生长发育阶段林地土壤肥力状况及地力衰退趋势,分析影响地力衰退的主要影响因子,以不同林龄(2、4、6、8和11 a)杉木短周期小径材培育人工林的土壤样本为研究对象,检测土壤有机质、全氮、全钾、全磷、水解性氮、速效钾、有效磷、有效铁、交换性钙、交换性镁含量和pH值共11个土壤肥力指标,分析不同林龄土壤养分差异及其变化趋势。结果表明:除土壤pH值外,土壤主要肥力指标的变化幅度较大,有机质、全氮、水解性氮、速效钾、有效磷含量在不同土层和不同林龄间均存在极显著差异(P<0.01);随着林龄的增长,林地土壤有机质和氮磷肥消耗较大,其中,表土层(0~20 cm)有机质、水解性氮、有效磷含量到11 a主伐时的降幅均超过30%,但不同林龄间各肥力指标差异不显著;采用改进后的Nemerow法计算的土壤肥力综合指数为0.412~0.530,土壤肥力等级为Ⅲ级(P_综<0.9),土壤肥力较低,相比较而言,土壤中有机质和氮肥含量较充足,而交换性阳离子极为贫乏;有机质、速效钾、水解性氮、有效磷含量与综合肥力指数呈显著或极显著正相关(R_偏~2>0.8),是影响土壤肥力的主要因子;土壤肥力综合指数随林龄增大而降低,11 a主伐期比造林初期(2 a)下降22.26%,但不同林龄间各单项肥力指数及综合肥力指数均不存在显著差异(P>0.08)。在该栽培模式的超短经营周期内,随着林龄增大及林木生长对土壤养分的消耗,林地土壤肥力出现一定程度的退化现象,但并未达到显著水平,有必要通过提高整地质量、改良土壤、林粮间作、增施有机肥及氮磷钾肥等措施适度增加土壤肥力,维持林地长期生产力。     

Response of soil microbial biomass and community composition to chronic nitrogen additions at Harvard forest

Soil microbial communities may respond to anthropogenic increases in ecosystem nitrogen (N) availability, and the microbial response may ultimately feed back on ecosystem carbon and N dynamics. We examined the long-term effects of chronic N additions on soil microbes by measuring soil microbial biomass, composition and substrate utilization patterns in pine and hardwood forests at the Harvard Forest Chronic N Amendment Study. Functional and structural genes for important N cycling processes were studied using DNA community profiles. In the O horizon soil of both stands, N additions decreased microbial biomass C as determined by chloroform fumigation-extraction. Utilization of N-containing substrates was lower in N-treated pine soils than in the controls, suggesting that N additions reduced potential microbial activity in the pine stand. Counts of fungi and bacteria as determined by direct microscopy and culture techniques did not show a clear response to N additions. Nitrogen additions, however, strongly influenced microbial community DNA profiles. The ammonia monooxygenase gene (amoA) generally was found in high N-treated soils, but not in control soils. The nifH gene for N₂-fixation was generally found in all soils, but was more difficult to amplify in the pine N-treated soil than the controls, suggesting that the population of N₂-fixers was altered by N additions. The 16S rDNA gene for Nitrobacter was found in all samples, but distinct differences among DNA profiles were observed in the pine B horizon in the control, low N, and high N-treated plots. Our findings indicate that chronic N additions decreased chloroform microbial carbon and altered microbial community profiles. These changes in microbial community structure may be an important component of the response of terrestrial ecosystems to human-accelerated N supply.     

Thinning intensity affects microbial functional diversity and enzymatic activities associated with litter decomposition in a Chinese fir plantation

Microbial functional diversity and enzymatic activities are critical to maintaining material circulation during litter decomposition in forests. Thinning, an important and widely used silvicultural treatment, changes the microclimate and promotes forest renewal. However, how thinning affects microbial functional diversity and enzymatic activities during litter decomposition remains poorly understood. We conducted thinning treatments in a Chinese fir plantation in a subtropical region of China with four levels of tree stem removal (0, 30, 50, and 70%), each with three replicates, and the effects of thinning on microbial functional diversity and enzymatic activities were studied 7 years after treatment by collecting litter samples four times over a 1-year period. Microbial functional diversity and enzymatic activities were analyzed using Biolog Ecoplates (Biolog Inc., Hayward, CA, USA) based on the utilization of 31 carbon substrates. Total microbial abundance during litter decomposition was lower after the thinning treatments than without thinning. Microbial functional diversity did not differ significantly during litter decomposition, but the types of microbial carbon-source utilization did differ significantly with the thinning treatments. Microbial cellulase and invertase activities during litter decomposition were significantly higher under the thinning treatments due to changes in the litter carbon concentration and the ratios of carbon and lignin to nitrogen. The present study demonstrated the important influence of thinning on microbial activities during litter decomposition. Moderate-intensity thinning may maximize vegetation diversity and, in turn, increase the available substrate sources for microbial organisms in litter and promote nutrient cycling in forest ecosystems.     

杉木人工林生态系统太阳辐射特征研究

根据2008年的观测数据,分析了会同杉木人工林太阳辐射各分量的时间分布特征及不同季节太阳辐射分配率的差异。结果表明:除大气逆辐射(RLd)外,其余3种辐射的年变化大致呈单峰形;太阳总辐射(RSd)及反射辐射(RSu)日变化均呈规则的单峰曲线,而森林生态系统长波辐射(RLu)和RLd则呈波浪形;就全年太阳辐射的分配率而言,净辐射(23.5%)最大,有效辐射(19.0%)次之,反射辐射最小,仅7.5%;不同季节太阳辐射分配率亦有所不同,反射辐射和净辐射均为旱季小于雨季,有效辐射则相反。     

亚热带地区杉木人工林和阔叶林土壤活性有机质研究(英文)

土壤活性有机质对土壤养分如氮、磷、硫的生物化学循环具有作用,其含量和质量影响土壤的初级生产力。本试验在中国科学院会同森林生态实验站通过对第一代、第二代杉木纯林和地带性阔叶林土壤活性有机质组分的对比研究,发现杉木纯林土壤活性有机质的含量低于地带性阔叶林。第一代杉木纯林易氧化有机碳、微生物生物量碳、水溶性有机碳和水溶性碳水化合物的含量分别比第二代杉木纯林高35.9%、13.7%、87.8%和50.9%,比地带性阔叶林的低15.8%、47.3%、38.1%和30.2%。在调查的三种林地内,土壤微生物生物量碳和水溶性有机碳含量下降幅度较大,其次为水溶性碳水化合物,而易氧化有机碳的变化最小。同时,杉木纯林土壤养分等理化性质也比地带性阔叶林低。这表明在杉木纯林取代地带性阔叶林以及杉木纯林连栽后林地的土壤肥力降低。图3 表2参26。     

杉木冷冻干燥材和气干材液体浸注性的比较研究

A comparative study was conducted on liquid penetration of the freeze-drying and air-drying sapwood and heartwood lumber of plantation Chinese fir （Cunninghamia lanceolata）. The maximum amount of dyeing solution uptake by the capillary rise method was used to evaluate the liquid penetration properties of the treated wood. The pit aspiration ratio was determined by semithin section method. Changes in wood microstructure were investigated using scanning electron microscopy. The results showed that compared with air drying, the freeze drying had a significant effect on liquid penetration of sapwood and heartwood of Chinese fir. The liquid penetration of sapwood is significantly higher than that of the heartwood for both drying treatments. Low pit aspiration ratio and cracks of pits membrane of some bordered pits are the main reasons for increasing liquid penetration after freeze drying treatment.     

Effects of phenolic acids on soil nitrogen mineralization over successive rotations in Chinese fir plantations

Phenolic acids are secondary metabolites of plants that significantly affect nutrient cycling processes.To investigate such effects,the soil available nitrogen(N)content,phenolic acid content,and net N mineralization rate in three successive rotations of Chinese fir plantations in subtropical China were investigated.Net N mineralization and nitrification rates in soils treated with phenolic acids were measured in an ex situ experiment.Compared with first-rotation plantations(FCP),the contents of total soil nitrogen and nitrate in second(SCP)-and third-rotation plantations(TCP)decreased,and that of soil ammonium increased.Soil net N mineralization rates in the second-and third-rotation plantations also increased by 17.8%and 39.9%,respectively.In contrast,soil net nitrification rates decreased by 18.0%and 25.0%,respectively.The concentrations of total phenolic acids in the FCP soils(123.22±6.02 nmol g^-1)were 3.0%and 17.9%higher than in the SCP(119.68±11.69 nmol g^-1)and TCP(104.51±8.57 nmol g^-1,respectively).The total content of phenolic acids was significantly correlated with the rates of net soil N mineralization and net nitrification.The ex situ experiment showed that the net N mineralization rates in soils treated with high(HCPA,0.07 mg N kg^-1 day^-1)and low(LCPA,0.18 mg N kg^-1 day^-1)concentrations of phenolic acids significantly decreased by 78.6%and 42.6%,respectively,comparing with that in control(0.32 mg N kg^-1 day^-1).Soil net nitrification rates under HCPA and LCPA were significantly higher than that of the control.The results suggested that low contents of phenolic acids in soil over successive rotations increased soil net N mineralization rates and decreased net nitrification rates,leading to consequent reductions in the nitrate content and enhancement of the ammonium content,then resulting in enhancing the conservation of soil N of successive rotations in Chinese fir plantation.     

Effects of continuous nitrogen addition on microbial properties and soil organic matter in a Larix gmelinii plantation in China

Continuous increases in anthropogenic nitrogen(N) deposition are likely to change soil microbial properties, and ultimately to affect soil carbon(C) storage.Temperate plantation forests play key roles in C sequestration, yet mechanisms underlying the influences of N deposition on soil organic matter accumulation are poorly understood. This study assessed the effect of N addition on soil microbial properties and soil organic matter distribution in a larch(Larix gmelinii) plantation. In a 9-year experiment in the plantation, N was applied at100 kg N ha~(-1) a~(-1) to study the effects on soil C and N mineralization, microbial biomass, enzyme activity, and C and N in soil organic matter density fractions, and organic matter chemistry. The results showed that N addition had no influence on C and N contents in whole soil. However,soil C in different fractions responded to N addition differently. Soil C in light fractions did not change with N addition, while soil C in heavy fractions increased significantly. These results suggested that more soil C in heavy fractions was stabilized in the N-treated soils. However,microbial biomass C and N and phenol oxidase activity decreased in the N-treated soils and thus soil C increased in heavy fractions. Although N addition reduced microbial biomass and phenol oxidase activity, it had little effect on soil C mineralization, hydrolytic enzyme activities, d13 C value in soil and C–H stretch, carboxylates and amides, and C–O stretch in soil organic matter chemistry measured by Fourier transform infrared spectra. We conclude that N addition(1) altered microbial biomass and activity without affecting soil C in light fractions and(2) resulted in an increase in soil C in heavy fractions and that this increase was controlled by phenol oxidase activity and soil N availability.     

杉木中幼龄人工林胸径结构及预估模型

分析了不同年龄杉木同龄林胸径结构变化规律,并建立了林分胸径因子与年龄因子的回归模型,联合正态分布模型可预估不同年龄的杉木同龄林胸径结构。     

Characteristics of carbon and nitrogen of soil microbial biomass and their relationships with soil nutrients in Cunninghamia lanceolata plantations

The soil microbial biomass and nutrient status under the native broadleaved forest and Cunninghamia lanceolata plantations at the Huitong National Research Station of Forest Ecosystem (in Hunan Province, midland of China) were examined in this study. The results showed that after the native broadleaved forest was replaced by mono-cultured C. lanceolata or C. lanceolata, soil microbial biomass and nutrient pool decreased significantly. In the 0–10 cm soil layer, the concentrations of soil microbial carbon and nitrogen in the broadleaved forest were 800.5 and 84.5 mg/kg, respectively. These were 1.90 and 1.03 times as much as those in the first rotation of the C. lanceolata plantation, and 2.16 and 1.27 times as much as those in the second rotation of the plantation, respectively. While in the 10–20 cm soil layer, the microbial carbon and nitrogen in the broadleaved forest were 475.4 and 63.3 mg/kg, respectively. These were 1.86 and 1.60 times as much as those in the first rotation, and 2.11 and 1.76 times as much as those in the second rotation, respectively. Soil nutrient pools, such as total nitrogen, total potassium, NH₄ ⁺-N, and available potassium, also declined after the C. lanceolata plantation replaced the native broadleaved forest, or Chinese fir was planted continuously. Less litter and slower decay rate in pure Chinese fir plantation were the crucial factors leading to the decrease of soil microbial biomass and nutrient pool in this area. Human disturbance, especially slash-burning and site preparation, was another factor leading to the decrease. There were significant positive correlations between soil microbial carbon and nitrogen and soil nutrients. To improve soil quality and maintain sustainable productivity, some measures, including planting mixed conifer with hardwood, preserving residues after harvest, and adopting scientific site preparation, should be taken. Translated from Chinese Journal of Applied Ecology, 2006, 17(12): 2,292–2,296 [译自: 应用生态学报]     

杉木混交林计算机辅助造林设计系统研究

通过对不同立地条件下杉木混交树种和不同经营水平的造林密度的研究，建立杉木交林计算机辅助造林设计系统。