共查询到20条相似文献，搜索用时 46 毫秒
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热激蛋白与园艺植物的耐冷性 总被引：2，自引：0，他引：2
园艺植物受到一定时间的高温胁迫处理后其耐冷性提高 ,这与热激条件下诱导产生的新蛋白(即热激蛋白 )有关。综述了热激蛋白及其与园艺植物抗冷性关系的研究进展 相似文献
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热激蛋白与植物的抗逆性 总被引：13，自引：0，他引：13
热激蛋白Heat-Shock Protein(HSP)又称热休克蛋白或应激蛋白(Heat-Stress Protein),是细胞在应激原(如高温)刺激下所生成的一组蛋白质. 相似文献
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高温胁迫下糙皮侧耳胞内钙离子对热激蛋白基因表达的调控 总被引：1，自引：0，他引：1
以糙皮侧耳(Pleurotus ostreatus)菌株CCMSSC00389为材料,研究高温胁迫(40℃、120min)下钙离子的响应及其对热激蛋白的调控。在高温胁迫条件下,在培养基中添加胞内钙离子螯合剂、胞外钙离子螯合剂、钙调蛋白抑制剂、质膜钙通道抑制剂、三磷酸肌醇受体拮抗剂,利用钙离子特异性荧光探针检测菌丝体胞内钙离子含量,采用荧光定量PCR检测热激蛋白的基因表达量。结果表明,高温胁迫促进胞内钙离子浓度升高,增加Hsp60、Grp78、Hsp90、Hsp104等4种热激蛋白的基因表达量;培养基中添加钙离子螯合剂或抑制剂会降低胞内钙离子浓度,抑制这些热激蛋白的基因表达。研究结果表明高温胁迫会诱导糙皮侧耳胞内钙离子浓度升高,参与信号转导,从而调控热激蛋白的表达来缓解高温胁迫引起的伤害。 相似文献
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热处理提高采后杜果抗冷性与蛋白质含量变化的关系 总被引：11，自引：0，他引：11
以‘紫花’卡亡果为试材,探讨了采后热处理提高抗冷性的效果及其与蛋白质含量变化的关系。结果表明：38oC 热空气处理0.5 ~3 d极显著地提高了紫花卡亡果抗冷性;热处理提高了卡亡果的蛋白质含量,并且热处理的卡亡果在2oC低温贮藏及室温后熟期间蛋白质含量也保持较高水平;热处理提高了热稳定蛋白在可溶性蛋白中所占的比例。这些结果表明,热处理导致的卡亡果蛋白质含量增加与抗冷性的提高有关,热处理诱导产生的热稳定蛋白是使卡亡果具有抗冷性的重要原因。 相似文献
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A lot of diseases (e.g., acute infarction and infections) and the diversity of harmful environmental changes, including elevating temperature, heavy metals and oxidants damage many kinds of proteins in organism. The denatured proteins usually evoke endogenous adaptive cellular mechanisms called heat shock response. After the activation, heat shock factors (HSFs) bind with heat shock element (HSE). This progress induces heat shock protein (HSP) expression, then facilitates repair of misfolded proteins, and finally inhibits the cell death (both necrosis and apoptosis) pathways. This review will introduce the structures and functions of HSF, HSP and HSE and details on the signaling process of HSP regulation by HSF. 相似文献
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热激减轻柿果冷害及其与脂氧合酶的关系 总被引：7，自引：0，他引：7
为研究采后柿果实冷害发生机理,探讨热激延缓采后柿果冷害的技术措施,以扁花柿果为试材,研究了经48℃热空气处理3h后,在1℃下冷藏时,热激对柿果冷害、脂氧合酶活性和膜脂脂肪酸含量的影响。结果表明,热激延缓了脂氧合酶活性的上升,其棕榈酸和硬脂酸的相对含量低于对照,而亚油酸和亚麻酸相对含量反而高于对照,并降低柿果冷害指数,但对油酸相对含量则无明显影响。这表明,热激减轻柿果冷害可能与抑制脂氧合酶活性和提高果实亚油酸和亚麻酸相对含量有关。 相似文献
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AIM: To observe effects of homocysteine and antagonized effects of taurine on electronic leakage and free radical production in myocardial mitochondria. METHODS: Myocardial mitochondria of rat heart was isolated, and was broken by supersonic wave to prepare submitochondria. Recombinant of succinic acid cytochrome c reductase was prepared with mitochondria of porcine heart. They were co-incubated with homocysteine and/or taurine with various concentration. The H2O2 and O2- were determined by chemiluminescence methods. The taurine transporter of heart mitochondria and its propert, and effects of homocysteine on its function were studied with glass filter. RESULTS: Homocysteine stimulated oxygen free radical production in heart mitochondria, submitochondria, and succinic acid cytochrome c in a concentration-dependent manner. Although taurine itself did not affect oxygen free radical production, taurine did inhibit oxygen free radical production in mitochondria, submitochondria and succinic acid cytochrome c in a concentration-dependent manner. Taurine transporters of Na+-dependent were existed in mitochondria membrane. Homocysteine inhibited taurine transtport in mitochondria in a concentration-dependent manner. CONCLUSIONS: Taurine inhibited electronic leakage and oxygen free radical production induced by homocysteine in electron transport chain. There were taurine transporters in mitochondria membrane, and transport functions of taurine transporter were inhibited by homocysteine. 相似文献
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西瓜野生种质耐冷性基因连锁的RAPD标记 总被引：24，自引：0，他引：24
运用ＲＡＰＤ技术进行了西瓜野生材料ＰＩ４８２３２２耐冷性基因连锁的分子标记的研究,找到了一个分子连锁标记ＯＰＧ１２／１９５０与耐冷基因连锁,其遗传距离为６．９８ｃＭ。 相似文献
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AIM:To study the relationship between myocardial HSP70 and PKC during myocardial ischemic preconditioning(IPC). METHODS: PKC inhibitor, polymyxin B(PMB) and PKC activator, phorbol 12-myristate 13-acetate(PMA) were applied to the models of myocardial ischemia/reperfusion in vivo and in vitro in rabbits, respectively, and the ventricular functions, PLA2 in the serum, and the expression of mycardial HSP70 mRNA were examined. RESULTS:IPC decreased PLA2 activity, improved the left ventricular function and increased the expression of myocardial HSP70 mRNA. Howerer, all these effects of IPC could be blocked by PMB. Interestingly, PMA mimicked IPC with attenuating the injuries of cardial myocytes and increasing myocardial HSP70 mRNA expression. CONCLUSION:PKC is involved in the myocardial HSP70 expression in case of ischemic preconditioning. 相似文献
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QIAN Shi-kun MIN Jun CHU Zhong-hua LI Dong-yin SHAO Jing WEI Jing OU Qing-jia 《园艺学报》2004,20(7):1190-1194
AIM: To study the effect of heat shock protein (HSP) on the maturation of dendritic cells (DCs) and observe the morphological changes of DCs dynamically. METHODS: The HSP (gp96)-peptide complex was purified from the tissues of hepatocellular carcinoma. Hepatoma cells were treated by heat shock for preparation of the HSP expressed on cell surface and then marked with DiI fluorescence. Immature DCs from peripheral blood mononuclear cells were cocultured with two types of HSPs. The morphological changes of DCs were observed dynamically and the effects of HSPs on the maturation of DCs analyzed by Flowcytometer. RESULTS: The morphological changes and the processes of antigen capture of DCs cocultured with DiI marked tumor cells were well showed. Four ways to capture antigens of DCs were observed, including direct contact, besieging, forming bubbles and extending pseudopodia with bubbles on the terminals. Results also indicated that both types of HSP could promote the maturation of DCs. CONCLUSION: DiI is a good fluorescent dye suitable for the morphological studies of DCs. Four ways for DCs to capture antigens were indicated in this paper. Different types of HSP, purifed from tumor tissues or expressed on tumor cells surface, promote DC maturation, and the purified HSP is more effective. 相似文献
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辣椒热胁迫及耐热性研究进展 总被引：1，自引：0，他引：1
热胁迫已经成为辣椒生产中不可忽视的环境因子.从热胁迫对辣椒生长发育的影响、辣椒耐热性的鉴定及提高辣椒耐热性的措施等方面,对近年来国内外有关辣椒耐热性研究的进展进行了综述,为进一步揭示辣椒耐热机制和提高辣椒耐热性的研究提供思路和参考.热胁迫下辣椒叶片净光合速率(Pn)、气孔导度(Cs)和蒸腾速率持续下降,花粉和花药正常发育受阻,抗氧化系统受到破坏,胞内Ca2+分布异常,激素比例改变,但使用耐热品种可减轻热胁迫的影响.辣椒耐热性的鉴定指标主要包括胁迫类、保护类、光合作用类和生殖类指标等.由于涉及的代谢过程较多,辣椒耐热性需从多方面进行综合评价.提高辣椒耐热性的主要措施有热锻炼、外源化学物质处理、培育耐热品种等,其中培育耐热品种是最经济有效,同时也是对生态环境最友好的方法. 相似文献