鸭疫里默氏菌的分离鉴定及病理组织学观察

从吉林某鸭场疑似鸭疫里默氏菌感染病/死鸭的脑部、心血分离到一株革兰阴性小杆菌,经细菌形态、生理生化、病理学观察、血清型鉴定与动物回归等试验,结果均证实分离株为RA,并将其命名为JL-RA1。该分离株对青霉素、替米考星、氟苯尼考高度敏感;而对头孢他啶、头孢曲松、磷霉素等多种药物完全耐药。     

异育银鲫嗜水气单胞菌的分离鉴定与病理组织学观察

为鉴定吉林省某养殖场所养殖异育银鲫(carassius auratus gibelio)死亡原因,在濒死鱼的肝、胰、脾、肾脏分离得到一株优势菌,经细菌形态、常规理化特性鉴定、人工感染试验、16S r RNA基因序列测定、病理解剖学及病理组织学观察,结果表明:分离株为气单胞菌属的嗜水气单胞菌,并将其命名为JL-PW-3。该分离株对环丙沙星、氟苯尼考、强力霉素、氯霉素等高度敏感;对庆大霉素、阿米卡星、阿莫西林、阿奇霉素、红霉素、恩诺沙星、磺胺间甲氧嘧啶等完全耐药。     

雏鹅感染烟曲霉鉴定与病理组织学观察

为确定荣昌3个养鹅场雏鹅感染的真菌种类,本研究采集病死雏鹅肺脏病变组织样本进行病原分离,对分离的真菌进行了形态学鉴定和ITS2序列PCR扩增及测序分析。结果显示:分离的真菌菌落颜色、菌丝结构、孢子无性繁殖方式均符合烟曲霉的特征,ITS2序列与GenBank的烟曲霉AF454113、AF454114、AY660923菌株同源性均为100%。分离菌株雏鹅回归试验显示,感染雏鹅气囊有真菌结节,肺泡壁增厚,毛细血管淤血,支气管管腔有脓性纤维蛋白或粘液,肝细胞变性坏死,淋巴细胞浸润等病变。肺脏主要表现为非典型肺炎,肝脏主要表现为实质性肝炎。本研究为临床诊断曲霉病提供了实验依据。     

Rapid method for the examination of platelet morphology

Oocysts were recovered from chickens experimentally infected with the Mukteswar strain of Newcastle disease virus and Eimeria acervulina or E tenella. Oocysts sterilised by sodium hypochlorite solution were washed, ruptured and inoculated into embryonating eggs which were examined after five days by the haemagglutination test for virus. All these tests were negative. Virus, initially detected with oocysts separated from faeces, was no longer present after sporulation in 2 per cent potassium bichromate. The B1 strain of virus survived for up to 30 hours in 2 per cent sodium hydroxide and 2 per cent potassium bichromate but was quickly destroyed by sodium hypochlorite (specific gravity 1.075).     

Direct complement fixation and isolation attempts for detecting Chlamydia psittaci infection of psittacine birds

Serum and fecal specimens from 147 birds were tested for Chlamydia psittaci. Isolation rates ranged from 3.4% of 88 birds that had titers less than 8, to 50% of six birds that had titers greater than or equal to 128. Testing of 44 paired sera revealed a significant titer increase in 5 pairs, a significant titer decrease in 8 pairs, and a stable titer in 31 pairs. The examination of both feces and serum is recommended for determining Chlamydia psittaci infections in individual live psittacine birds.     

A practical method for the production of Brucella skin test antigen

Skin test antigens for the diagnosis of brucellosis were produced from the rough Brucella abortus strain 45/20. The production of two products termed Brucellin B and Brucellin W are described. The method described for the production of Brucellin W is recommended as an improved practical method of Brucellin production. The Brucellin products described were equal in sensitivity to that of a commercially available product, Brucellergen, which is used in New Zealand. Brucellin B was extensively tested in non-infected cattle and its specificity was equal to that of Brucellergen. Recommendations for the standardisation of skin test reagents for the diagnosis of brucellosis are made. Intradermal testing for brucellosis in cattle should be used only for the identification of infected herds and not as an individual animal test.     

A simple specimen preparation method for histopathological evaluation of vestibular organs

Vestibular organs consist of the maculae staticae, which are located in both the utricle and saccule, as well as the semicircular ducts and their ampullas. There have been no reports on specimen preparation methods for vestibular organs, including maculae staticae or semicircular ducts. In this study, we investigated highly reproducible methods of preparing vestibular organ specimens for histopathological examinations. We established a method that allows researchers to observe the utricle and saccule, including otoliths, the ampulla of a semicircular duct, and parts of semicircular ducts. This highly reproducible method is useful for histopathological analysis of mice with symptoms of abnormal equilibrium caused by medical toxicity and genetic modification.     

简单、实用、高效的细胞培养技术

通过近几年的实践,笔者对我国兽医生物制品生产中传统的细胞培养工艺进行了一些改进,包括用洗洁精代替其他洗液,用自行研制的营养液代替常规营养液用于细胞培养,改变培养时的转速,改变细胞消化液,改进细胞的保存方法.这些方法均成功应用于生产,取得了良好的效果.     

防暴犬攻击持枪案犯实战技能训练方法初探

在2004年度全警岗位大练兵的热潮中,我们对使用防暴犬攻击制服持枪案犯的实战技能进行了训练研究探索,取得了初步成果,并在第二届全国警犬技术比赛开幕式上进行了汇报演练。现将训练方法介绍如下：     

利用原位二步灌流法分离鸡肝细胞

试验选用15~18日龄的粤黄鸡苗,采用胶原酶原位二步灌流的方法分离肝细胞。对小鸡破坏大脑处死后,打开腹腔,先用PBS液对小鸡进行门静脉快速灌流,当肝脏呈现苍白略带黄色时改用Ⅳ型胶原酶溶液继续灌流,直至肝包膜与肝组织出现裂隙,即可分离出肝细胞。结果表明:用原位二步灌流法可得到大量活性高且分离较纯的鸡肝细胞。     

The vascularization of the epididymis of the boar with special reference to perfusion fixation

Vasculature of the epididymis was investigated by means of corrosion casts. In the boar, epididymal arteries form a complex network around their stem vessel, the testicular artery. Proper perfusion fixation or complete casting therefore require direct injection into one of these branches. To reach the distal cauda, cannulation of the deferential artery is further needed. Connections between all of these feeding vessels occur at the level of the vascular cone. A prominent anastomosis between an epididymal branch and the testicular artery is regularly observed under the caput epididymis. Epididymal veins drain to a large extent into the pampiniform plexus. Unlike the situation in other species, vascularization of testis and epididymis are closely associated in the boar.     

犬股骨骨折的内固定法

骨折是骨或软骨的完整性或连续性因外力机械性作用而遭受破坏.骨折常伴有周围软组织不同程度的损伤.在小动物外科疾病中,骨骼与关节疾病比较常见,其中以骨折,尤其后肢股骨骨折发病率为最高,约占全部骨折的32%[1-3].