猪增生性肠病的流行病学及防控

猪增生性肠病(PPE),又称猪增生性肠炎、猪回肠炎(Porcine lleitis),猪增生性肠道病、猪肠腺瘤样病、猪增生性出血性肠炎,但目前普遍称之为猪增生性肠病.猪增生性肠病是猪的一种接触性肠道传染病,1931年美国首次报道该病,至今已呈全球性流行,并给养猪业造成巨大经济损失.     

猪增生性肠病的流行病学及防控

猪增生性肠病（PPE）,又称猪增生性肠炎、猪回肠炎（Porcine Ileitis）、猪增生性肠道病、猪肠腺瘤样病、猪增生性出血性肠炎,但目前普遍称之为猪增生性肠病。猪增生性肠病是猪的一种接触性肠道传染病,1931年美国首次报道该病,至今已呈全球性流行,并给养猪业造成巨大经济损失。世界许多国家和地区,如澳大利亚、墨西哥、加拿大、意大利、丹麦、比利时、巴西、阿根廷、韩国、我国台湾省等均有发病报道。     

泉州市规模化养猪场猪增生性肠病的流行特点及控制方案

猪增生性肠病(Porcine Pro-liferative Enteropathy,PPE)又称增生性回肠炎或猪回肠炎。猪增生性肠病是以回肠的黏膜呈腺瘤样增生     

猪增生性肠炎及其防控

猪增生性肠病（PPE）,又被称作猪增生性肠炎、猪回肠炎、猪增生性肠道病、猪肠腺瘤病、猪增生性出血性肠炎（PHE）等,这一名称是对所有发病猪的基本病变的准确描述。1993年发现胞内劳森菌是所有临床急性和慢性增生性肠炎的唯一致病菌后,这一命名更为确立（McOrist等,1993）。     

猪增生性肠炎及其防控

猪增生性肠病（PPE）,又被称作猪增生性肠炎、猪回肠炎、猪增生性肠道病、猪肠腺瘤病、猪增生性出血性肠炎（PHE）等,这一名称是对所有发病猪的基本病变的准确描述。1993年发现胞内劳森菌是所有临床急性和慢性增生性肠炎的唯一致病菌后,这一命名更为确立（McOrist等,1993）。     

猪增生性肠炎的研究进展

猪增生性肠炎（porcine proliferative enteritis,PPE）,又称猪增生性肠病（porcine roliferative nteropathy,PPE）,是以回肠和结肠隐窝内未成熟的肠细胞发生腺瘤样增生为特征的猪的一种肠道综合症。本文主要概述其痛原学、流行病学、发病机理、临床症状、病理变化、诊断以及防治等方面的研究现状。     

福建省部分猪场猪增生性肠病病原学调查与分析

<正>猪增生性肠病(Porcine Proliferative Enteropathy,PPE)又称增生性回肠炎或猪回肠炎。是以回肠和结肠隐窝内未成熟的肠细胞发生腺瘤样增生为特征的猪的接触性传染病。增生性肠病是一种在急性     

一例猪增生性出血性回肠炎的诊治

猪回肠炎是由胞内劳森菌（Lawsonia intracellularis）感染所引起的回肠、盲肠、结肠增生、肥厚并可能伴随肠黏膜出血的一种非常重要的肠道疾病。根据临床表现可分为慢性型、亚临床型与急性型。慢性型与亚临床型又称猪增生性回肠炎,主要影响猪只的生长性能,而临床症状不典型。急性型又名增生性出血性回肠炎,可引起生长肥育猪及后备母猪出血性下痢、贫血,甚至突然死亡。2011年4月份以来笔者接诊过几起增生性出血性回肠炎的案例,现将其中一例报告如下。     

猪增生性肠炎的诊治

猪消化道疾病是猪常见的疾病,给养猪业带来的经济损失较大。猪增生性肠炎又称坏死性肠炎或肠腺癌变,目前国内外有许多关于猪增生性肠炎的报道,研究者们认为该病可能与弯杆菌属未定种有关。本文报道了几例猪增生性肠炎的临床观察与治疗,以供同仁们和广大饲养者参考。     

细胞内罗森氏菌对猪健康和性能的危害

猪增生性肠病常被称为猪回肠炎,是猪的肠道传染病,特征是细胞内罗森氏菌导致肠细胞增生造成小肠粘膜增厚。     

猪增生性肠炎研究进展及防控措施

胞内劳森菌为严格细胞内寄生的无芽孢细菌,革兰氏染色阴性,抗酸染色阳性。主要感染3~20周龄猪而引起"猪增生性肠炎",临床表现慢性间歇性下痢或急性出血性下痢,排焦油样黑色粪便或血便。病理变化为小肠后段和结肠前段肠黏膜层增厚隆起,形成特征性分枝状皱折,肠腺上皮剧烈增生,形成畸形排列的分枝状肠腺。本病可进行流行病学、临床特征、病原学、病理学、血清学、分子生物学诊断,并需要与猪痢疾、猪梭菌性肠炎、仔猪副伤寒等病进行临床鉴别。本病的防控,应从猪群管理、引种、消毒、药物预防等方面考虑,接种疫苗是行之有效的措施。     

A descriptive study of the frequency and characteristics of proliferative enteropathy in swine in Ontario by analyzing routine animal health surveillance data

Routine surveillance data, collected on pathology submissions at the Animal Health Laboratory in Guelph between 1992 and 1997, were analyzed to determine demographic, clinical, and pathologic characteristics of cases of proliferative enteropathy and the frequency of this condition relative to other infectious enteric diseases in swine in Ontario. The most commonly reported disease was Escherichia coli enteritis (average cases/year = 70.0). Among infectious enteropathies that occur typically in neonatal pigs, coccidiosis (28.4 cases/year) and rotaviral enteritis (5.6 cases/year) were reported. Among infectious enteropathies generally associated with diarrhea in weaner and grower/finisher pigs, the most frequently reported was proliferative enteropathy (27.6 cases/year), followed by swine dysentery (23.3 cases/year), transmissible gastroenteritis (19.6 cases/year), and salmonellosis (8.4 cases/year). Diarrhea and bloody diarrhea were reported in 29% and 31%, respectively, of herds diagnosed with proliferative enteropathy. Important gross intestinal lesions included mucosal hypertrophy (62% of cases), hemorrhage (47%), and mucosal necrosis (34%). Histologic intestinal lesions included epithelial hyperplasia (90% of cases), mucosal necrosis (59%), and inflammation (49%). Our results suggest that proliferative enteropathy is a major infectious enteric disease in grower/finisher pigs in Ontario.     

The use of quantitative PCR for identification and quantification of Brachyspira pilosicoli, Lawsonia intracellularis and Escherichia coli fimbrial types F4 and F18 in pig feces

This field study explored the cytokine expression in intestinal tissue and serum from 19 diarrhoeic and 9 healthy pigs in herds with a long-time history of Lawsonia intracellularis-infection. The disease, proliferative enteropathy (PE), is associated with diarrhoea and poor performance in growers and haemorrhagic diarrhoea and sudden death in finisher pigs, but the immunopathology is poorly understood. Histopathology, demonstration of L. intracellularis and porcine circovirus type 2 (PCV2) in intestinal tissue by PCR, and detection of serum antibodies to L. intracellularis, were performed. The presence of IL-1β, IL-6, IL-10, IFN-α, IFN-γ, TNF-α and TGF-β in sera was determined by immunoassays, and intestinal mRNA expression of these cytokines plus IL-12p40 was determined by qPCR. Intestinal specimens from pigs with intestinal adenomatosis (n=2), proliferative haemorrhagic enteropathy or swine dysentery (n=2), and controls (n=2) were analysed by a genome wide porcine microarray. The clinical signs of PE were not always supported by the subsequent analyses, and the presence of PCV2 may have contributed to an increased mRNA expression for IFN-γ in intestinal specimens from some pigs. The limited gene expression in the microarray analyses and the limited expression of cytokines in both sera and intestines, indicate that the immune response is poorly activated in the initial course of an infection with L. intracellularis. However, the gene encoding for insulin-like growth factor binding protein 3 (IGFBP-3) was up-regulated in two pigs with prominent mucosal proliferation.     

Species-specific DNA probes for Campylobacter species isolated from pigs with proliferative enteritis

Cloned, chromosomal DNA probes from porcine isolates of Campylobacter hyointestinalis and C. mucosalis were developed for the detection and identification of these putative swine enteric pathogens. High molecular weight chromosomal DNA from each species was used to construct genomic libraries in plasmids. Recombinants were selected which hybridized strongly to the homologous organism, but not to any other species of Campylobacter. Species-specific recombinants were labeled with phosphorus-32 and tested for sensitivity by dot blot hybridization to various dilutions of DNA and bacteria from each swine species, including C. hyointestinalis, C. mucosalis, C. coli and C. jejuni. Specificity was tested by hybridizing these probes against various strains of C. hyointestinalis or C. mucosalis, and against reference strains of all other described Campylobacter species. A C. hyointestinalis-specific probe and a C. mucosalis-specific probe were identified which were capable of detecting 1 ng of DNA or 10(4) cfu by bacterial spot blotting on nylon membranes. These probes hybridized to intestinal mucosal scrapings containing C. hyointestinalis and C. mucosalis obtained from pigs with proliferative enteritis, but not to material from normal pigs. Thus, cloned, chromosomal DNA probes may be useful in the detection and identification of bacteria involved in swine proliferative enteritis.     

Enterocecocolitis associated with intraepithelial Campylobacter-like bacteria in rabbits (Oryctolagus cuniculus)

We examined 28 suckling, weanling, and young adult rabbits with lethargy, inappetence, and mucinous, semifluid feces. Sixteen of the rabbits had intestinal lesions. In eight of these rabbits, the primary changes were multifocal to diffuse epithelial proliferation and accumulation of lymphocytes, macrophages, or both in the lamina propria of the small intestine, cecum, and sacculated colon. In two of these rabbits, the accumulation of macrophages in the lamina propria was extensive. The other eight rabbits had erosive and suppurative cecocolitis, and four of the rabbits with proliferative lesions also had suppurative cecocolitis. In Warthin-Starry-stained sections of affected intestine, curved or spiral bacteria were visible within degenerated or hyperplastic epithelium, in luminal exudate, or in both. Such organisms were sparse or not found in the other 12 rabbits, which did not have intestinal lesions. The bacteria ultrastructurally resembled intraepithelial Campylobacter-like bacteria previously observed in proliferative enteritis in a variety of species and in acute typhlitis in young rabbits. In immunofluorescence tests, Campylobacter-like bacteria in epithelial cells, crypt lumina, and in luminal exudates in both proliferative and erosive lesions bound monoclonal antibodies and polyclonal antisera prepared against intracellular bacteria found in proliferative enteritis in pigs, hamsters, and ferrets. These observations indicate that a condition similar to proliferative enteritis of swine, hamsters, and other species also occurs in laboratory rabbits.     

Porcine proliferative enteritis: serological, microbiological and pathological studies from three field epizootics.

Three outbreaks of porcine proliferative enteritis were evaluated clinically, pathologically, microbiologically and serologically. The disease was characterized by a chronic intermittent diarrhea. Pathological lesions included a thickened, turbid ileum with the microscopic appearance of proliferating ileal crypt epithelial cells. Comma shaped intracytoplasmic organisms were observed in the apical portions of the proliferating crypt epithelial cells with a Warthin-Starry silver stain. Microbiologically, both Campylobacter sputorum subspecies mucosalis and Campylobacter hyointestinalis, were cultured from ileal specimens of seven pigs with lesions of porcine proliferative enteritis. Microagglutination antibody titers were determined on sera from 12 of 14 pigs with porcine proliferative enteritis and on sera from 91 clinically normal swine. Pigs with porcine proliferative enteritis had a low antibody titer to subspecies mucosalis that ranged from 1-3 with a mean of 2.17. A varied C. hyointestinalis titer from 3-7 with mean of 4.83 was determined. Titers to either subspecies mucosalis and C. hyointestinalis were higher in non-porcine proliferative enteritis pigs. The results indicate that the presence of a positive titer to either C. hyointestinalis or subspecies mucosalis in swine is not indicative of clinical disease. The isolation of C. hyointestinalis from diseased ileal specimens (porcine proliferative enteritis) confirms previous reports implicating this agent in the disease.     

猪传染性胃肠炎临床诊断及防治

猪传染性胃肠炎是由猪传染性胃肠炎病毒引起的急性高度接触性肠道传染病,临床上以腹泻、呕吐和脱水为特征,各种年龄的猪都可发病,对哺乳仔猪的危害最严重.然而以腹泻为主症的还有猪痢疾、仔猪黄痢、仔猪白痢、仔猪红痢(梭菌性肠炎)、流行性腹泻、轮状病毒感染、仔猪副伤寒等7种疫病,为了更有效地防控猪传染性胃肠炎,做到早诊断、早治疗.论文阐述了猪传染性胃肠炎与猪其他7种腹泻疫病的临床症状的区别及猪传染性胃肠炎的防治要点.     

Use of embryonating eggs for isolation of Campylobacter species from intestines of swine with proliferative enteritis

Intestinal tissues from swine affected with proliferative enteritis were ground, filtered through a 0.65-micron pore membrane filter, diluted, and injected into 7-day-old embryonated hens' eggs via the yolk sac. At 2, 4, and 7 days later, yolk sac swab specimens taken from live embryos were cultured for Campylobacter species. Campylobacter hyointestinalis was recovered from eggs injected with tissues of swine with acute hemorrhagic proliferative enteritis at dilutions up to 10(-4). Campylobacter mucosalis was recovered from eggs injected with tissues of swine with chronic proliferative enteritis at dilutions up to 10(-6). Campylobacter coli was recovered from several specimens without lesions of proliferative enteritis and also from some specimens with lesions of proliferative enteritis. Two previously undescribed hemolytic Campylobacter species designed as hemolytic number 1 and hemolytic number 2 were recovered from normal and experimentally inoculated swine tissues. Few contaminating organisms grow in eggs and these were usually recovered at dilutions of 10(-2) or less. Recovery of Campylobacter species by use of these techniques was seldom successful in tissues stored at -70 C for more than 6 months.     

Treatment of proliferative colitis in ferrets

Proliferative colitis associated with intracellular Campylobacter sp was diagnosed in 10 ferrets. The ferrets had a history of diarrhea (often blood-tinged or mucoid), dehydration, and chronic weight loss. Additional clinical signs included rectal prolapse, lethargy, fever, and a palpably thick colon. In 5 ferrets, the diagnosis was confirmed by colonic biopsy, via endoscopy. Supportive treatment in 5 ferrets did not alleviate the clinical signs or the proliferative intestinal disorder. oral chloramphenicol treatment (50 mg/kg of body weight, q 12 h for 10 to 21 days) resulted in marked clinical improvement and eradication of proliferative intestinal lesions in 5 ferrets.