Mist propagation of peach and almond × peach hybrids

Several factors contributing to the successful rooting of stem cuttings of four peach clones and one almond × peach hybrid under intermittent mist were tested. With the almond × peach cross, leaf-bud cuttings were also tested.Severe cutting back of adult peach mother trees in winter favoured rooting of the cuttings, but less severe cutting back induced maximum roots per cutting.For short periods vermiculite was found to be a suitable medium. Sand alone or mixed with vermiculite or gravel gave poor results. Gravel alone or mixed with vermiculite was intermediate. For growing the rooted cuttings for a longer period, a mixture of perlite and peat was very suitable.A period of illumination of 3 h starting at midnight with incandescent light improved rooting of peach cuttings in August and October, but not in June.With cuttings obtained from old fruit-bearing peach trees highest rooting rates were obtained in July, but best root development occurred when rooting was carried out in October. In July rooting rate of basal cuttings was much higher than that of terminal ones. Success with leaf-bud cuttings (including a small branch piece) obtained from young mother trees of the almond × peach hybrid was only achieved at the end of May or in June.Dipping the base of peach cuttings in water before rooting was of advantage with one cultivar rooted in September, but of no advantage with another cultivar rooted in June.When the base of stem cuttings was dipped for a prolonged period in IBA solutions of various concentrations, highest rooting rates were obtained with 25–50 ppm IBA for peaches and with 200 ppm for the almond × peach hybrid. The addition of Phygon XL to this solution was of some advantage for peach cuttings. The concentration inducing maximum root development was higher than that required for maximum rooting and callusing. The optimal IBA concentration for rooting of almond × peach leaf-bud cuttings was 100 ppm.Penetration of the IBA into the leaf-bud cuttings reached a maximum 45 min a after floating them on a 100 ppm solution.Transplanting cuttings which had been rooted under mist was somewhat difficult; however, high rates of survival were obtained with cuttings planted in September which had developed a good root system.     

不同基质与植物生长调节剂对扶桑插条生根的影响

研究了珍珠岩、蛭石、河沙、菜园土、腐殖营养土等不同基质配比、不同生长素调节剂(IBA、IAA、NAA)及其浓度、生长调节剂与多效唑(PP333)混合使用对扶桑插条生根的影响。结果表明:河沙+腐殖营养土等体积混合的基质对生根的效果最好;IBA处理的生根效果较好,其中以IBA 150mg/L处理的生根率最高,为93.67%;IAA的处理中,以低浓度50mg/L的生根率最高,为90.67%;较高浓度NAA的处理平均生根数较多,但生根率较低。用含PP33320mg/L与IAA 50mg/L或与IBA 150mg/L的混合液处理均能提高扶桑插条的生根效果。     

Cloning of Ceylon olive (Elaeocarpus serratus L.) using conventional methods

Ceylon olive (Elaeocarpus serratus L.; family Elaeocarpaceae) is an under-utilised edible fruit tree that is sparsely distributed in the southern peninsula of the Indian sub-continent. Fresh and ripened fruit are edible and are used to prepare value-added products such as squash, jams and pickles. Methods to produce clonal E. serratus plants using softwood cuttings, air-layers, and grafts were investigated. Softwood cuttings were collected during the wet summer season (June–August) and showed 96.7% rooting success following 2.5 mM indole-3-butyric acid (IBA) treatment for 3 h. Hardwood cuttings collected during the wet summer season and subjected to IBA treatment failed to root. The duration of the auxin treatment significantly influenced the rooting percentage of softwood cuttings. Seasonal variations in the rooting response of softwood cuttings was also noted. The wet summer (June–August) was the best season for rooting softwood cuttings. Air-layers of hardwood branches, prepared during the wet summer season by pre-treating with 7.5 mM IBA resulted in 87.1% rooting. The spliced grafting technique could be applied also to elite Ceylon olive clones, with 70% and 50% survival rates of softwood and hardwood grafts, respectively. Ceylon olive could therefore be cloned by adopting these methods. Propagation based on softwood cuttings would facilitate moderate-scale cloning of this valuable, elite germplasm.     

Production of high quality Ardisia plants by stem tip cuttings

To produce commercially acceptable Ardisia plants, stem tip cuttings from mature plants were rooted and forced in greenhouses. Ten centimeter long cuttings were either treated with 200 ppm 1-naphthalene acetic acid (NAA) for 2 h, 2000 ppm indole-3-yl-butyric acid (IBA) for 10 s, or 0.5 and 1.0% IBA powder prior to sticking them in the rooting medium. Rooting percentage at 45 days exceeded 76% with 2000 ppm IBA treatment which was a significant increase over non-treated control. Rooted cuttings developed into three types of plants: those forming only vegetative shoots without flowers, those forming reproductive shoots with flowers, and those forming both vegetative and reproductive shoots. The ideal plant produced only vegetative shoots when rooted cuttings were transplanted into pots. About 50% rooted cuttings were forced to finish, producing 31 or 40% of high quality plants when rooted cuttings with vegetative shoots were grown in a greenhouse (GH) at temperatures higher than 21/19 °C (day/night) in 1995 or 21/18 °C GH in 1997, respectively. This method shortened the total production time to less than 2 years as compared to 4 years when starting from seeds.     

三种生长调节剂对金边虎尾兰、月季与天竺葵扦插生根的影响

以金边虎尾兰、月季与天竺葵为试材,研究不同生长调节剂及其浓度对3种园林植物扦插生根的影响。结果表明:金边虎尾兰叶基段以100mg/L浓度的IBA或NAA扦插效果较好,叶尖段以IBA 100～200mg/L或NAA 200mg/L扦插效果较好,生根率均达到100%;100～200mg/L浓度的IBA与100mg/L浓度的NAA对月季茎插生根效果较好,生根率达75.6%～77.8%;天竺葵茎插各处理的生根率均达到100%,但从平均生根数上比较,100mg/L NAA或25mg/L IBA对其扦插生根效果最佳,25～300mg/L的IAA对其生根数有抑制作用。     

Collection time,cutting age,IBA and putrescine effects on root formation in Corylus avellana L. cuttings

The effects of collection time, cutting age, indole-3-butyric acid (IBA) and putrescine application on the rooting of cuttings of Italian hazelnut cultivars ‘Tonda Gentile Romana’, ‘Tonda di Giffoni’ and ‘Nocchione’ were investigated. Samples collected during late June, late July and early September from newly formed and 1-year old part of twigs to be utilized to produce leafy cuttings, after being treated with 1000 and 2000 ppm IBA, respectively. In addition, the September cuttings were also treated, respectively, with 1000 and 2000 ppm IBA and 1600 ppm putrescine. Rooting ability was evaluated 2 months after planting for each treatment and collection time. Satisfactory rooting of hazelnut leafy cuttings was observed when collection time occurred in June and September, whereas leafy cuttings collected in July showed a limited capacity of rooting in all cultivars tested. On average, the rooting of the newly formed leafy cuttings was more than the 1-year old cuttings. Rooting was also promoted by IBA treatments, mainly in ‘Nocchione’ and ‘Tonda di Giffoni’. In contrast, young cuttings collected from ‘Tonda Gentile Romana’ in early September rooted poorly when treated with IBA alone, but showed the best rooting (∼80%) after the application of a combination of 1000 ppm IBA and 1600 ppm putrescine. The current findings confirm that putrescine can be a useful substance for increasing rooting percentage and root quality in cuttings of some hazelnut cultivars as obtained from ‘Tonda Gentile Romana’ cultivar.     

Growing season and hydrogen peroxide effects on root induction and development in Olea europaea L. (cvs ‘Frantoio’ and ‘Gentile di Larino’) cuttings

Semi-hardwood cuttings of ‘Frantoio’ (high rooting ability) and ‘Gentile di Larino’ (low rooting ability) cultivars were obtained from 1-year-old olive shoots sampled in mid-August during the 2000 and 2001 growing season. Semi-hardwood cuttings were dipped in IBA 4000 ppm and H₂O₂ (0%-control or 3.5% w/v) solutions before rooting in greenhouse equipped with an automatic mist system. At 57 and 88 days after the beginning of rooting treatments cuttings were scored for the presence of callus, roots and roots number. In both cultivars and years, the IBA 4000+H₂O₂ treatment significantly modified rooting of cuttings at 57 days in comparison with IBA 4000 treatment. The positive effects of H₂O₂ on rooting were gradually smoothed after 88 days. At the end of the propagation cycle (88th days), cuttings treated with IBA 4000+H₂O₂ had significantly higher root number in comparison with those treated with IBA 4000 alone.     

外源IBA对辣椒下胚轴不定根发生的调控作用

为了探明吲哚丁酸(Indole-3-butyric acid,IBA)对辣椒(Capsicum annuum L.)下胚轴不定根发生的诱导机制,采用IBA浸蘸后扦插的方法,研究了外源IBA处理后辣椒下胚轴不定根发生进程、糖类物质转化与不定根发生相关基因表达情况。结果表明:外源IBA诱导辣椒下胚轴不定根发生具有浓度依存效应,150μmol·L~(-1) IBA处理后不定根发生区段长度、不定根数及平均根长分别是对照的3.47倍、3.28倍和1.91倍;150μmol·L~(-1) IBA上调了不定根启动相关基因Ca ARL1和Ca Cyc B1的表达,下胚轴的不定根原基发生区域明显增加;此外,150μmol·L~(-1) IBA还上调了下胚轴不定根发生区段糖代谢相关基因Ca CWI和Ca STP的表达,可溶性糖、淀粉含量及细胞壁转化酶活性显著提高。结果表明适宜浓度IBA处理可以促进辣椒下胚轴不定根发生区段糖类物质的积累和转化,诱导辣椒下胚轴不定根的发生。     

Accelerated rooting of carnation ‘Red Baron’ by temperature pre-treatment

Rooting of carnation cuttings was promoted by storage temperatures above 0°C with an inverse relationship between the level of temperature and the duration of the treatment. The effect of temperature on root dry weight was far more important than the effect of duration of storage. A long storage and a high temperature increased the incidence of Fusarium. Storage at temperatures lower than 13°C gave better results with auxin applied after storage, while temperatures higher than 13°C gave better results with a pre-treatment of auxin. During storage for 14 days at 9°C, the position of the cuttings had no clear effect on rooting. Horizontal storage, however, appeared to be unfavourable. Removal of 0.5 cm of the base of the stem after storage nullified the storage effect. Carbohydrates in the removed pieces decreased during storage, more so at 13°C than at 0°C. Anatomically, an acceleration of cell division at the base of the cutting was observed during storage at 9°C as compared to 0°C.At a commercial nursery, storage of cuttings for 12 days at 14°C improved rooting by 84% compared to storage at 0°C. The presented technique tends to shorten the expensive and vulnerable period from planting to rooting.     

A simple method for large-scale propagation of Dendrocalamus asper

The influence of position of cuttings, auxin treatments and seasonal variation on adventitious rooting of Dendrocalamus asper, a multipurpose edible bamboo of horticultural value, was examined. Single node proximal (culm) and distal (culm-branch) cuttings were collected during the months of April–June and treated for 24 h with water or 2 mM auxin (indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)). The treated cuttings were planted horizontally in sand beds and maintained under misting conditions for 2 months. Culm cuttings exhibited better adventitious rooting than culm-branch cuttings and the month of May proved to be the best for rooting of both types of cuttings. Auxin treatments also influenced adventitious rooting and their effectiveness was in the order: NAA>IBA>IAA. The interaction among auxin, position of cuttings and month of collection was also found to be significant on root induction and differentiation. Single node culm cuttings, without auxin treatment, resulted in 98% plantlets in May and 67% plantlets in June, and emerged as a suitable material for large-scale vegetative propagation. Administration of IAA or NAA to abundant culm-branch cuttings of low commercial utility (collected in May) also produces ≥50% plantlets.     

Effect of Position of IBA Application on the Rooting of Plum Hardwood Cuttings

The rooting ability of plum rootstock hardwood cuttings inserted for one month in a peat-grit medium at 70 °F basal temperature was influenced by the depth to which they were previously dipped in an alcoholic solution of 4(indolyl-3) butyric acid (IBA). Although in detail the response varied with IBA concentration and rootstock clone, in general the highest proportion of cuttings rooted when only the basal cut end of the shoot was treated. The detrimental effect of dipping to one inch increased with increasing concentration of IBA and was shown to be caused by the application of IBA to the epidermis, since it could be eliminated by sealing the epidermis with polyvinyl resin prior to dipping or by reducing the concentration of IBA applied to the epidermis relative to that applied to the cut end. Epidermal application was only beneficial when insufficient stimulation to rooting was obtained through basally-applied IBA.     

IBA和发根农杆菌对糠椴嫩枝扦插的影响

 研究了IBA和发根农杆菌处理对糠椴嫩枝扦插生根的影响, 并对其影响生根的机理进行了初步探讨。结果表明: IBA和发根农杆菌处理显著( P < 0.01) 提高了插条生根率, 增加了成活苗的生根数和根长, 明显缩短了生根时间。IBA和发根农杆菌处理还显著( P < 0.01) 提高了插条生根期内源GA₃和IAA、可溶性总糖和全氮的含量及IAA /ABA比值, 降低了ABA含量, 对糠椴嫩枝扦插生根有明显的促进作用, 其中以500 mg·kg^-1 IBA +发根农杆菌处理的生根效果最好, 插条的生根率、成活苗的生根数、成活苗的根长分别比对照增加了122.08%、65.75%和23.63% , 生根时间比对照缩短了14 d。     

A comparison of conventional cloning options for annatto (Bixa orellana L.)

Summary

Annatto (Bixa orellana L.) is a tree indigenous to tropical America that has been naturalised throughout the Indian sub-continent. The plant is chiefly valued for its carotenoid pigments, annatto. Methods for the clonal propagation of hardwood and softwood cuttings, air-layering, budding, and grafting of annatto were investigated. Hardwood cuttings collected during the wet Summer months (June-July) were treated with indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), or naphthaleneacetic acid (NAA) at varying concentrations. The highest percentage rooting (63.4%), along with highest number of roots (8.33 per cutting) were achieved by hardwood cuttings after 12 h of 2.5 mM IAA treatment. Among the various auxin treatments of softwood cuttings, 5.0 mM IBA in combination with 5.0 mM NAA for 5 min resulted in a significant (P < 0.05) rooting percentage (56.7%). The responses of softwood cuttings to an auxin analogue (boric acid) and/or to thiamine hydrochloride (vitamin B) were inferior to synthetic auxins. When subjected to air-layering, hardwood branches gave 100% rooting without any application of hormone. Among the different types of budding methods attempted (patch, T, or I), patch budding produced the highest efficiency (78%) of propagation. Splice-grafting could also be used for conventional propagation of annatto, with a 50% survival rate. Annatto can therefore be cloned by adopting these methods. Propagation based on softwood cuttings facilitated moderate-scale cloning of this valuable, elite germplasm.     

Effects of forcing,etiolation and indole butyric acid on rooting of cuttings of Artocarpus heterophyllus Lam.

Rooting of cuttings of jackfruit (Artocarpus heterophyllus Lam.) has been successfully induced through forcing of shoots, etiolation and application of indole butyric acid (IBA) under mist spray. Maximum rooting (84%) and maximum survival (50–75%) were obtained with the forced and etiolated shoots treated with 5000 mg/l IBA. The next best treatment was the forced and etiolated shoots treated with 10 000 mg/l IBA, which was somewhat similar to forced, non-etiolated shoots treated with 5000 mg/l IBA. In the control without IBA no success was obtained even under forcing and etiolation.     

Effect of indolebutyric acid and sampling-dates on the rooting of four Tilia taxa

Softwood and semi-hardwood cuttings of 4 Tilia taxa were taken at different times and treated with high concentrations of IBA. Rooting-response varied with taxa and sampling-date. Rooting in Tilia americana L. cuttings was maximum when taken on 19 May and 1 June. Cuttings of Tilia cordata Mill. did not differ significantly in rootability when taken on 19 May, 22 June or 13 July, but rooting was significantly less with 3 August cuttings. Tilia × euchlora Koch cuttings did not root. All Tilla × euchlora Koch ‘Redmond’ cuttings taken on 19 May or 22 June rooted. A 5-second basal dip of IBA significantly stimulated rooting, especially in the range of 20 000–35 000 mg 1^?1.     

激素在常春藤水插中的应用

利用生根剂NAA和IBA处理常春藤插穗,通过在扦插15 d调查愈伤情况,在30 d调查生根情况,包括根长、根数、成活率等指标。结果表明:2种生根剂结合起来(各50%)处理,浓度为30 mg/L和50 mg/L生根效果最佳。     

Nitric oxide in combination with indole-3-butyric acid improves root growth in ‘Ferdor Julior’ hardwood cuttings (Prunus insistitia (L.) × Prunus domestica (L.))

The production of plants for high density fruit orchards requires a highly efficient clonal propagation method. ‘Ferdor Julior’ rootstock is well adapted to different soil conditions, but its natural rooting potential is very limited. Propagation treatments, such as bottom heat and indole-3-butyric acid (IBA) applications have previously been used to improve rooting. The aim of this work is to evaluate the effects of either IBA, or sodium nitroprusside (SNP; a nitric oxide (NO) donor), or a combination of both on the rooting of hardwood cuttings of ‘Ferdor Julior’. As metabolism markers, ascorbic acid (AA) and glutathione (GSH) were measured in adventitious roots. ‘Ferdor Julior’ hardwood cuttings were treated as follows: untreated, 0.3 mM IBA, 1 mM SNP, 1 mM SNP previously exposed to light (as a negative control), and 1 mM SNP + 0.3 mM IBA. After 4 months, the combined use of SNP and IBA had increased root growth and lateral rooting, but resulted in less shoot growth, whilst AA and GSH concentrations were also reduced. IBA and SNP individual treatments showed intermediate results, compared to untreated cuttings. According to the results obtained, there may be an additive effect of auxin and NO signaling pathways. In conclusion, this new and promising technique for ‘Ferdor Julior’ propagation could improve lateral root development and promote early lateral root growth.     

不同激素处理对香石竹扦插生根的影响

研究不同激素种类和浓度处理对香石竹扦插生根的影响。结果表明:使用IBA、NAA浸泡香石竹插穗可以促进生根。其中NAA与IBA各处理相比根重与根长有极显著差异。对插穗基部进行处理,以基部撕裂结合NAA浸泡效果最好。以75、150mg/L的NAA浸泡插穗效果最好。     

不同基质对聊红槐扦插生根的影响

以细河沙、草炭、珍珠岩:草炭=1∶1为扦插基质,用400 mg/L的IBA和NAA处理聊红槐插穗,进行春季硬枝扦插,测定其生根指标,筛选出适合生根的扦插基质.结果表明:以河沙为基质,经过IBA处理过的插穗的生根率较NAA处理的好;以草炭为基质,经过NAA处理过的插穗较IBA处理的好;以珍珠岩:草炭=1∶1为基质,经过IBA处理过的插穗较NAA处理的好;同一激素处理的根系效果,浸泡时间3h的优于1h处理的.     

Rooting of Berberis and Ligustrum cuttings from stock plants grown at selected light intensities

Plants of Berberis thunbergii DC., B. thunbergii DC, ‘Atropurpurea’, Ligustrum obtusifolium Seibold and Zucc. var. regelianum (Koehne) Rehd., and L. × vicaryi Beckett were grown at 37, 53, 70 and 100% full sunlight to determine rootability of cuttings. Rooting hormone levels of 0, 0.1, 0.3 and 0.8% IBA in talc were applied to the cuttings, and % rooting, root length, and fresh and dry weights were recorded. Optimum rooting of cuttings of Berberis resulted when stock plants were grown at 70% light, with 37% light being the next most successful. Optimum rooting of cuttings of Ligustrum obtusifolium var. regelianum resulted when stock plants were grown at 37% light. Rooting of Ligustrum × vicaryi did not respond to light intensity. An interaction between rooting-hormone and light intensity was noted for root length of Ligustrum species.