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J. P. Hudson 《The Journal of Horticultural Science and Biotechnology》2013,88(1):27-43
Some varieties of raspberries can readily be propagated from root cuttings. The method is more rapid than the traditional use of root suckers and might be useful in the initial multiplication of raspberry clones. Recommendations are made regarding choice and preparation of parent root material, method of making and planting cuttings, and subsequent management of the plants produced. The behaviour of raspberry root cuttings at various seasons and in different environments is described and discussed. 相似文献
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G. G. Thomas E. H. Wilkinson 《The Journal of Horticultural Science and Biotechnology》2013,88(2):115-123
Black currants are readily propagated from single-bud hardwood cuttings approximately i inch long, with the bud at the top and planted vertically. The best part of the shoot from which to take such cuttings is the lower- mid portion (buds 15-22).Optimum rooting is obtained from cuttings planted in January, February and March after dormancy has broken in the field, but by using 24-hour day-length successful rooting can be extended from October to March. The emergence of flowers in March-planted cuttings slightly reduces rooting.Thirteen commercial varieties have been propagated successfully by this method.Suitable softwood and semi- hardwood cuttings are available when the current season's shoots are 12 nodes or more in length, i.e. normally in late April. When these are planted in a propagation frame with soil heating, good rooting is obtained from April to June provided the subtending leaf is retained during the rooting period.Twenty-four-hour day-length increases the rooting of cuttings planted in July; lack of rooting after July is attributed to early leaf abscission, the initiation of flowers and the onset of dormancy. 相似文献
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D. A. Richards 《The Journal of Horticultural Science and Biotechnology》2013,88(3):192-199
Many investigations at the West African Cacao Research Institute require the production of uniform clones of cacao. Although Amelonado, which is the name adopted for the most predominant variety of cacao in West Africa, appears to be genetically uniform, it has been considered advisable to use self-rooted clones for such detailed work as the definition of virus symptomatology. Also many of the Institute’s disease and pest resistant selections are known to be heterozygous types of cacao, and investigations on these must of necessity be carried out on clonal material. Cacao can be rapidly propagated by budding, but this method introduces the variability associated with seedling rootstocks. 相似文献
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激素处理和取穗枝条部位对长春花扦插生根的影响 总被引:1,自引:0,他引:1
以盆栽红色长春花为试材,研究了激素处理和取穗枝条部位对长春花扦插生根的影响.结果表明:用单一激素NAA、IBA、ABT-6进行处理时,以50 m/L ABT-6处理的扦插生根效果较好,生根率达63.70%,平均生根数4.31条,平均根长1.27 cm;50 mg/L ABT-6溶液浸泡9h效果优于其它时间处理,生根率达82.96%,平均生根数6.61条,平均根长2.40 cm;2种激素组合以ABT-6 50 mg/L+NAA 15 mg/L最好,生根率达100%,平均生根数11.76条,平均根长4.68 cm;不同取穗部位插穗的扦插效果,中部>下部>上部,中部生根率达100%,平均生根数12.51条,平均根长5.01 cm. 相似文献
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IBA对文冠果硬枝扦插根系形态指标的影响 总被引:1,自引:0,他引:1
对文冠果硬枝扦插苗生根根系形态特征进行定量分析,研究其根系总长度、根系总表面积、总体积等根系形态指标及其与IBA浓度变化的关系.结果表明:与清水对照(CK)相比较,IBA显著(P<0.05)提高了插穗根系总根长、总表面积、总体积形态指标;随着IBA浓度的升高,根系各形态指标呈现先升高后降低的趋势,总根长和总表面积峰值均出现在IBA浓度为800 mg/L,且生根率达到了65.40%;经IBA处理的插穗生根根系主要分布在径级>2.0 mm区间;根系总根长、总表面积、总体积与IBA浓度相关性均达到显著性水平.经回归分析,当IBA浓度保持在795~820 mg/L时,文冠果插穗可获得较高的根系形态指标. 相似文献
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J. Amos R. G. Hatton T. N. Hoblyn R. C. Knight 《The Journal of Horticultural Science and Biotechnology》2013,88(3):248-258
SummaryThe effects of seed coat removal and chilling on the germination of seeds of ten cultivars of ornamental peach (Prunus persica Batsch) were investigated. Seeds were rinsed in running tap water for 48 h in order to facilitate seed coat removal. Only a few non-chilled, intact seeds germinated (e.g., 6% of ‘Hito’ seeds). Seed coat removal and no chilling resulted in some seed germination in eight of the ten cultivars, ranging from 6% to 83%. Chilling intact seeds at 5°C for up to 10 weeks resulted in greater frequencies of germination (average = 85% germination) than seed coat removal before (average = 80%) or after chilling (average = 73%). There was a linear decrease in the germination percentage with an increase in abscisic acid (ABA) content for ‘Yaguchi’,‘Kanpaku’, and ‘Kikumomo’ seeds (R = –0.66; P <0.001). The most consistent decrease in total seed ABA content (average of 64%) occurred during the 48 h rinsing period. In ‘Kanpaku’, dry seeds had an intermediate ABA content (13 ng per seed); but, in the embryonic axes, this increased from 0.03 ng to 1.2 ng per seed with an increase in the duration of the chilling period. The lowest germination percentages were found in this cultivar. These results suggest that ABA synthesis in the embryonic axes during chilling may affect the varietal characteristic of seed dormancy, and that rinsing seeds for >48 h could remove sufficient ABA to allow seed germination with minimum chilling. 相似文献
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用ABT和IBA,NAA,B1不同的配比对康乃馨插穗进行5种不同的扦插生根处理,结果表明:6 g/kg IBA 2g/ kg NAA 0.5 g/kg B1配比的生根粉的效果最为理想,生根率达97%以上,成苗率达85%. 相似文献
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Margaret Robertson C. A. Wood 《The Journal of Horticultural Science and Biotechnology》2013,88(3):231-234
Studies were carried out on the assumption that incompatibility and dwarfness of budded citrus trees result from impaired carbohydrate movement through the bud union. During autumn and winter, carbohydrates move from the tree tops to the roots and accumulate there as starch. The starch level can therefore serve as a measure of this movement.This study included seven rootstock varieties all budded with Shamouti orange scion. Unbudded rootstocks were included for purposes of comparison.The starch level was determined in the winter at three locations along the trunk, below and above the bud union.No clear indication was found of impaired carbohydrate movement through the bud union of stock-scion combinations showing very low growth vigour.The starch level in bark and wood in the rootstock portion of trunks of budded trees was found to be negatively correlated with tree size. This indicates that the level of starch in the trunk results from the growth vigour of the tree and is not a factor which affects it.No relation was found between starch level in the scion and tree size. Based on these findings the conclusion was reached that incompatibility and low growth vigour in citrus do not seem to result from impaired carbohydrate movement through the trunk and starvation of the roots. 相似文献
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低磷胁迫下平邑甜茶根构型与磷吸收特性的变化 总被引:5,自引:1,他引:4
为揭示苹果适应低磷胁迫的机制,以平邑甜茶[Malus hupehensis (Pamp)Rehd.]为材料,采用水培方式研究了缺磷和复磷条件下根系磷吸收和根构型参数的变化。结果显示,平邑甜茶幼苗在缺磷营养液中培养120 h期间,根系磷最大吸收速率始终高于对照,并趋向一个较稳定的差值;缺磷处理最初12 h内根系磷吸收米氏常数Km值提高,但72~120 h明显下降。缺磷处理的平邑甜茶幼苗转到完全营养液中培养12~120 h,根系对磷的转运效率影响不大,而与磷的亲和力下降,并逐渐恢复到正常状态。平邑甜茶幼苗在缺磷条件下培养至11 d时,一级侧根总长度低于对照,但在17~26 d时,一级侧根总长度明显提高。结果表明,平邑甜茶在短期内主要通过磷吸收动力学的变化来适应缺磷胁迫,较长时期则通过根构型的变化来适应。 相似文献
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A method has been developed for the large-scale propagation of lilies from callus cultures. Bulb scales, being available at all times, are an ideal source of explants. Callus was induced throughout the annual cycle of all the 12 Oriental hybrid cultivars tested, by treating the bulb scales with a combination of 5 μM BA and 5 μM 2,4-D. Once initiated, this callus grew vigorously on media lacking exogenous growth substances. Callus induction by BA and 2,4-D, and the subsequent growth of such callus in the absence of exogenous growth substances, occurs within a broad range of cultivars and species within the genus. The callus could be maintained on a medium lacking ammonium salts, but the maximum growth rate was obtained at 10 mM NH4NO3. At low (0.2 mM) or zero levels of NH4NO3, light as compared with darkness was inhibitory to growth.The callus maintained its capacity for organogenesis. Maximum production of plantlets was obtained in continuous light on agar medium with 0.5 μM NAA.Potentially, the successive use of liquid and agar cultures can produce 6 × 1012 plants per year from 1 g (dry wt.) of callus. Plantlets derived from callus were consistently diploid and could be readily transferred to soil. 相似文献
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The carbohydrate resources of softwood hop cuttings were modified by supplying sugars, in solution, during a 5-day pre-rooting dark period. At low light intensity, where cuttings were maintained at about compensation point, the effect of such sugar pre-treatment was to cause large increases in rooting, compared with cuttings of low initial carbohydrate status. At higher light intensity, however, where considerable accumulation of carbohydrate took place during the rooting period, the only effect of initial differences in carbohydrate status was in the time at which roots were first observed ; the subsequent rate of rooting and final number of roots were determined by the environmental conditions. 相似文献
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以对葡萄根瘤蚜敏感的‘克瑞森无核’葡萄(Vitis vinifera ‘Crimson Seedless’)组培苗和抗性砧木‘1103P’组培苗为试材,通过荧光定量PCR技术从扩展蛋白基因家族中筛选到经根瘤蚜侵染后表达上调的扩展蛋白基因家族成员VvEXPA1。采用RT-PCR方法克隆到VvEXPA1基因片段,连接至原核表达载体pET-32a中,转化大肠杆菌DE3菌株,测序确定构建的重组载体pET32a-VvEXPA1开放阅读框正确,并经IPTG诱导表达了融合蛋白。该蛋白以包涵体的形式存在,纯化后免疫新西兰兔,制备扩展蛋白多克隆抗体,通过Western-blot和ELISA检测抗体的特异性及效价。结果显示制备的抗体具有较高的特异性,ELISA显示效价为1:51 200。 相似文献