GC02鸡球虫病疫苗免疫对雏鸡血清自由基代谢的影响

将100只雏鸡随机分为试验组和对照组(150只/组),试验组于7、14日龄时各免疫接种8 000个GC02鸡球虫病疫苗卵囊.第2次接种后7、14、21、28 d时.随机选取试验组和对照组鸡各10只,颈静脉采血分离血清,测定血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活力和丙二醛(MDA)含量.结果显示:(1)试验组接种7、28 d时,血清SOD水平与对照组比较差异不显著(P>0.05);14 d时显著升高(P<0.05);21 d时极显著升高(P<0.01).(2)7、14、21、28 d时,试验组与对照组比较,血清CAT水平差异均不显著(P>0.05);(3)试验组与对照组比较,7 d和21 d时,血清GSH-Px水平差异不显著(P>0.05);14 d和28 d极显著升高(P<0.01);(4)7、14、21、28 d时,试验组与对照组比较,血清MDA含量差异均不显著(P>0.05).结果表明:接种GC02鸡球虫病疫苗能够提高鸡抗氧化功能.     

GC02鸡球虫病疫苗免疫效果的观察

试验研究了GC02鸡球虫病疫苗免疫预防鸡球虫病的效果.试验一选择100只肉杂鸡,饲养至21日龄时,随机分为A、B、C、D四组,其中A组免疫肉鸡球虫病疫苗,B组免疫GC02鸡球虫病疫苗,C组为不免疫攻虫组,D组为不免疫不攻虫组,A、B组于21日龄口服免疫球虫苗,A、B、C三组于28日龄时口服接种1×105个孢子化卵囊攻虫,于36日龄捕杀A、B、C、D组全部鸡只,以比较肉鸡球虫病疫苗与GC02鸡球虫病疫苗的预防效果.结果表明两种球虫病疫苗均能有效的抗雏鸡艾美耳球虫感染,且是安全有效的.试验二选择100只肉杂鸡,随机分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ五组.研究了GC02鸡球虫病疫苗不同免疫次数的保护作用,Ⅰ组于14日龄免疫GC02鸡球虫病疫苗(4 000个卵囊),Ⅱ组分别于7日龄(4 000个卵囊)、21日龄(8 000个卵囊)免疫GC02鸡球虫病疫苗,Ⅲ组分别于7日龄(4 000个卵囊)、14日龄(8 000个卵囊)、21日龄(12 000个卵囊)免疫GC02鸡球虫病疫苗,Ⅳ组为不免疫攻虫组,Ⅴ组为不免疫不攻虫组,于28日龄时,Ⅰ、Ⅱ、Ⅲ、Ⅳ组均口服接种1×105个孢子化卵囊攻虫,36日龄捕杀全部鸡只,观察GC02鸡球虫病疫苗不同免疫次数的免疫效果.结果表明2次免疫的效果优于1次免疫和3次免疫,2次免疫能有效的控制球虫病的发生,对雏鸡有明显的促生长作用.     

球虫病弱毒疫苗GC_(02)免疫接种对雏鸡脾脏组织结构的影响

通过建立球虫病弱毒疫苗GC02免疫接种雏鸡试验,研究GC02球虫病疫苗免疫接种对鸡的脾脏的影响。80羽1日龄肉杂鸡随机分为免疫接种组(试验组)和对照组(n=40)。免疫接种组于7日龄和14日龄各口服接种8 000个孢子化卵囊的球虫病疫苗,空白对照组接种生理盐水。在鸡21,28,35和42日龄分别于试验组和对照组中各取鸡10羽,颈静脉放血致死,立即解剖取脾脏,制作石蜡切片,显微观察。结果显示,攻虫后的短时间内脾脏细胞生长受到抑制,生长发育缓慢。随后,免疫应答明显,脾脏发育明显。     

用疫苗防鸡球虫病

据<农民日报>记者侯曜禹10月8日报道:鸡球虫病是危害极大的一种寄生原虫病,该病分布广泛,即使是在集约化的、封闭的或者笼养的大型养鸡场,"群居"的鸡也容易感染球虫病,给养鸡业造成巨大的经济损失.最近,上海农业科学院畜牧兽医研究所成功开发出了一种疫苗,用免疫方式预防鸡球虫病.     

用疫苗防鸡球虫病

肉鸡的球虫病可以通过服用抗球虫药物来预防。然而,耐药性球虫几乎可以使抗球虫药物不起任何作用。这是在比利时Tom Serraerens肉鸡养殖场观察到的情况。使用疫苗是他的最后一招。     

DLV疫苗预防鸡球虫病的效果

鸡球虫病是由艾美耳属的一种或多种球虫寄生于鸡肠上皮细胞内所引起的寄生虫病,几乎所有鸡场都存在本病。养鸡业对球虫病的防治一直是以药物为主。由于球虫很容易产生抗药性,必须经常更换抗球虫药。此外,应用药物防治球虫病会产生药物残留,食用有残留抗球虫药物的鸡肉会影响人体健康。由于球虫的抗药性日趋严重,从而导致了近年来人们对使用球虫疫苗感兴趣。为了提高养鸡生产的经济效益,我们采用球虫苗预防鸡球虫病,通过3批鸡的效果观察,我们认为DLV疫苗可以取代抗球虫药。现将试验结果报告如下。－．材料与方法1试验材料和动物分…     

鸡球虫病疫苗的研究进展

鸡球虫病是由艾美耳球虫感染鸡引起的一种危害极其严重的寄生原虫病。目前,控制球虫病主要还是依赖于药物,但由于球虫耐药株的频繁出现和人们对药物残留等问题的关注,使人们更加注重探讨从免疫预防的角度去控制球虫病。本文综述了鸡球虫病活疫苗(强毒疫苗和弱毒疫苗)的研究和应用现状及重组疫苗(DNA疫苗和重组蛋白疫苗)的研究进展,同时介绍了新型疫苗佐剂的研究情况。     

抗鸡球虫病疫苗研究的新进展

抗鸡球虫病疫苗研究的新进展李秉鸿广西兽医研究所５３０００１球虫抗药性以及控制球虫病的费用大的问题促使人们研究各种免疫方法预防鸡球虫病的热情增高。从最早的强毒活虫苗Ｃｏｃｃｉｖａｃ，Ｉｍ－ｍｕｎｏｃｏｘ，到新近的Ｌｉｖａｃｏｘ和Ｐａｒａｃｏｘ类早熟弱毒...     

雏鸭腔上囊的生长及组织发育

通过对1～49日龄雏鸭腔上囊绝对质量和生长指数测定以及组织学观察,探讨雏鸭腔上囊生长及组织发育规律。结果:随日龄增长,腔上囊绝对质量逐渐增高;腔上囊生长指数21日龄达最高;黏膜大皱褶高、宽度、淋巴滤泡面积、皮质宽度均不断增加;小结相关上皮向腔面形成突起,并不断增高增宽。结果表明,雏鸭腔上囊1～14日龄生长较为缓慢,14～35日龄生长较快,35～49日龄发育基本趋于稳定,其中21～28日龄是雏鸭腔上囊生长发育的高峰时期,28日龄时,雏鸭腔上囊组织结构基本发育成熟。     

益生菌对肉鸡法氏囊发育及淋巴细胞增殖的影响

试验旨在研究不同种类的益生菌制剂对内鸡法氏囊发育以及淋巴细胞增殖的影响.选用雄性0日龄817肉鸡75只,随机分成5组.空白对照组饲喂基础日粮,试验组分别添加牧丁宝Ⅰ型、牧丁宝Ⅱ型、丁酸梭菌和益芪康,试验周期21 d.结果 显示:与对照组相比,益芪康、牧丁宝Ⅰ、牧丁宝Ⅱ和丁酸梭菌组的法氏囊增重分别提高13.61％、9.0...     

Developmental changes in cell proliferation and apoptosis in the normal duck bursa of Fabricius

The aim of this work was to investigate developmental changes in cell proliferation and apoptosis in normal duck bursa of Fabricius using flow cytometry and immunohistochemistry. Studies were carried out on Tianfu ducks on days 24 and 27 of embryogenesis (E24 and E27) along with days 20, 70, and 200 of postnatal development (P20, P70, and P200). Results showed that the percentage of G₀/G₁ bursa cells significantly increased between E24 and P200 while the percentage of cells in the S phase or G₂ + M phase as well as the proliferating index obviously decreased during the same period. Proliferation cell nuclear antigen was detected in lymphocyte and interfollicular epithelium. The proliferative lymphocyte density tended to decrease from E24 to P200. Apoptotic bodies in macrophages, free apoptotic bodies, or nuclei with condensed chromatin in lymphocytes in follicles were identified by transferase-mediated dUTP nick-end labeling. Both flow cytometry and microscopic analysis reveal that the proportion of apoptotic cells and apoptotic lymphocyte density increased from E24 to P20, fell on P70, then rose again on P200. Our foundings demonstrate that cell proliferation decreases and apoptosis increases with age. These changes may account for duck bursa development and involution.     

B cell differentiation in the bursa of Fabricius and spleen of embryos and chicks immediately after hatching

Flow cytometric analysis and immunohistochemical observation were used to qualitatively and quantitatively clarify the nature of B cell differentiation in the bursa of Fabricius of chick embryos and to determine the timing of antibody class switching in chicken spleens based on positivity of IgM and IgG on and in the cells. In the bursa, the sIgM‐positive cell population formed from the 12^th to 15^th day of embryogenesis. The proportion of sIgM‐high expressing (sIgM^high) cells was lower among bursacytes than splenocytes of hatched chicks, suggesting that the sIgM^high bursacytes are to be released to peripheral sites. The proportion of sIgM^high cells was higher at 0 days old than at any other examined stage of development. Colonization of the spleen by B cells occurred between the 18^th day of embryogenesis and 0 days old. Antibody class switching was thought to start in the spleen between 1 and 2 weeks of age, because IgG‐positive cells were present in the spleen of 2‐week‐old chicks, but not 0‐day‐old or 1‐week‐old chicks.     

5种弥散神经内分泌细胞在鸭腔上囊中的表达特征

应用免疫组化技术并结合图像分析软件研究了胃泌素、β-内啡肽、胰高血糖素、5-羟色胺、生长押素在鸭腔上囊中的表达特征.结果显示鸭腔上囊小结相关上皮细胞呈胃泌素强阳性反应;滤泡间粘膜上皮呈β-内啡肽中强度阳性反应;淋巴滤泡皮质部有少量的β-内啡肽、胃泌素、5-羟色胺阳性细胞,呈中、强度免疫反应;淋巴滤泡髓质有胃泌素强阳性细胞;生长抑素和胰高血糖素均呈阴性反应.结果说明,鸭腔上囊中存在β-内啡肤、胃泌素、5-羟色胺弥散神经内分泌细胞,它们在淋巴滤泡皮质部的表达特征有利于其通过内分泌和旁分泌方式调节B细胞的发育.     

禽法氏囊三肽囊素免疫生物学及其同功能单链抗体研究进展

法氏囊（BF）是禽类特有的免疫器官，为B细胞发育、成熟提供微环境，是免疫球蛋白基因转变场所；其免疫活性物质为三肽囊素（Bursin)；它不仅分布于BF，还存在于胸腺Hassall小体、哈德氏腺、脾脏、BF T细胞区及骨髓；它能诱导家禽和哺乳动物B细胞前体分化；其靶器官是BF，能够拮抗IBD疫苗对BF的损伤，提高ND弱毒疫苗及油苗产生抗体水平；人工合成Bursin作用的靶器官也是BF，可促进BF发育、抗体的产生、维持母源抗体作用。     

神经肽Y及其Y1受体阳性细胞在鸭腔上囊中的分布及发育变化(英文)

神经肽Y及其Y1受体在神经-免疫-网路中发挥着重要作用。二者在许多脊椎动物胸腺和脾脏中的表达已有研究,但它们在腔上囊中的表达及发育变化特征还未见报道。本试验应用免疫组化技术研究神经肽Y及其Y1受体阳性细胞在鸭腔上囊胚胎及胚后发育中的分布及变化特征。结果显示,在鸭腔上囊中神经肽Y及其Y1受体分别最早表达于26和22d胚龄。神经肽Y阳性细胞分布于黏膜上皮、滤泡、肌层和小动脉平滑肌。Y1受体阳性细胞分布于黏膜上皮、滤泡淋巴细胞。各部位神经肽Y及其Y1受体阳性细胞数量表现出明显增龄变化特征。结果说明鸭腔上囊中存在神经肽Y及其Y1受体,二者可能存在的相互作用主要表现在胚后发育阶段,这与B淋巴细胞的发育与分化以及腔上囊的退化密切相关。     

Use of Yucca schidigera Extract in Broiler Diets and Its Effects on Performance Results Obtained with Different Coccidiosis Control Methods

A trial was conducted to assess the effects of the dietary supplement Yucca schidigera extract on the performance of broilers subjected to different coccidiosis control methods. Six hundred male broiler chicks were randomly allocated to 1 of 5 treatments with 4 replicates each. The treatments were as follows: T1, vaccinated against coccidiosis; T2, nonvaccinated, a dietary coccidiostat; T3, vaccinated against coccidiosis, diet supplemented with Y. schidigera extract (0.01%); T4, coccidiostat plus Y. schidigera extract added to feed (0.01%); and T5, nonvaccinated, nonsupplemented control. The parameters analyzed were bird performance and morphology of the intestinal mucosal surface. The intestinal villi were higher at 6 d of age in broilers treated with coccidiostats or vaccinated against coccidiosis and supplemented with Y. schidigera extract compared with groups receiving the vaccine only or no treatment. Addition of the Y. schidigera extract to the diet of vaccinated birds improved average daily gain and feed conversion rate at 42 d of age compared with the other treatments, suggesting there might be a beneficial, synergistic effect between the coccidiosis vaccine and the Y. schidigera extract.     

法氏囊弱毒疫苗GUMBORO PLUX对蛋鸡肉鸡免疫效果试验报告

应用法氏囊弱毒疫苗GUMBOROPLUX对蛋鸡和肉鸡进行免疫效果试验,试验结果表明:该疫苗用于蛋鸡8～12日龄首免,间隔10d加强免疫后,可获得强毒攻击的完全保护;疫苗免疫后5d观察法氏囊变化,囊体比和囊指数在安全范围内;疫苗免疫不影响ND抗体效价滴度,对机体不产生苗源性免疫抑制用。法氏囊弱毒疫苗GUMBOROPLUX用于肉鸡,16日龄一次免疫,可获得强毒攻击的完全保护;疫苗免疫后5d观察法氏囊变化,囊体比和囊指数在安全范围内;疫苗免疫不影响ND抗体效价滴度,对机体不产生苗源性免疫抑制用。