四倍体刺槐扦插生根过程中氧化酶活性的变化

四倍体刺槐(Robinia pseudoacacia)属难生根树种,为了解其生根过程中氧化酶活性的变化规律,用300,400和500 mg/L的吲哚丁酸(IBA)处理四倍体刺槐嫩枝插条,以清水浸泡为对照,测定扦插生根过程中体内吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)和过氧化物酶(POD)活性的变化。结果表明,各IBA处理和对照插穗IAAO和POD活性变化趋势相似,在四倍体刺槐生根过程中呈升-降-升-降的变化规律,而PPO酶则基本呈降-升-降-升的变化规律;不同处理间3种酶活性的平均值和各阶段酶活性差异均显著。说明在四倍体刺槐扦插生根过程中,不定根的发生和发展与3种酶活性有密切联系,不同浓度IBA处理对各酶活性影响显著,其中以500 mg/L IBA处理对四倍体刺槐扦插生根的促进效果更明显。     

音乐声频对植物生长素的影响

[目的]研究音乐声频对番茄等6种蔬菜内吲哚乙酸（IAA）含量的影响。[方法]试验设置音乐处理组和正常对照组,对番茄等6种蔬菜栽培进行统一管理,定期对相关生长指标进行测定,并同时采集样品,利用高效液相色谱（HPLC）法测定吲哚乙酸的含量。[结果]音乐声频处理对番茄等6种蔬菜内部IAA含量有显著的促进作用,声频处理后的6种蔬菜IAA含量都显著优于对照组。[结论]音乐声频能起到促进植物分泌IAA的作用。     

音乐声频对植物生长素的影响

[目的]研究音乐声频对番茄等6种蔬菜内吲哚乙酸（IAA）含量的影响。[方法]试验设置音乐处理组和正常对照组,对番茄等6种蔬菜栽培进行统一管理,定期对相关生长指标进行测定,并同时采集样品,利用高效液相色谱（HPLC）法测定吲哚乙酸的含量。[结果]音乐声频处理对番茄等6种蔬菜内部IAA含量有显著的促进作用,声频处理后的6种蔬菜IAA含量都显著优于对照组。[结论]音乐声频能起到促进植物分泌IAA的作用。     

Indoleacetic acid oxidase activity of apoperoxidase

The conventional activity of electrophoretically purified horseradish peroxidase toward guaiacol, pyrogallol, 2,6-dimethoxyphenol, and benzidine is abolished by removal of the heme prosthetic group with a mixture of cold acetone and hydrogen chloride. The apoenzyme, though devoid of peroxidase activity, retains its activity as an indoleacetic acid oxidase when it is supplied with 10(-5) mole of manganous ion and 2,4-dachlorophenol per liter. This oxidase activity is cyanide-sensitive; azide also inhibits under specific conditions of both pH and cofactor concentration. Partial restoration of the peroxidase activity by recombination of apoprotein with heme produces no effect on the oxidase activity, except that cofactors are no longer absolutely required. Therefore, it appears that the activity of peroxidase as an indoleacetic acid oxidase need not directly involve the heme prosthetic group, or that manganous ions and dichlorophenol can substitute for the heme group in the reaction between indoleacetic acid and oxidase.     

Auxin effects on the mobility of kinetin in the plant

Kinetin (6-furfurylaminopurine) is generally considered to be relatively immobile within plants. Kinetin labeled with (14)C was applied to the stumps of decapitated bean plants, with or without simultaneous application of indoleacetic acid. Significantly greater amounts of kinetin moved downwards in the stem in the presence of added indoleacetic acid than in its absence.     

Activation and Inhibition of Indoleacetic Acid Oxidase Activity from Peas

The inhibition of indoleacetic acid oxidase activity by chlorogenic acid is markedly variable. Aqueous extracts of acetone precipitates from etiolated pea epicotyls contain heat-stable substances which decrease the inhibition produced by chlorogenic acid and also have a small activating effect. Low concentrations of pcoumaric acid (1 micro/ml) can stimulate this action. The possible role of activators and inhibitors in the in vivo regulation of indoleacetic acid oxidase activity is discussed.     

Hormone-induced stabilization of soluble RNA in pea-stem tissue

When indoleacetic acid labeled with carbon-14 in the carboxyl group is fed to excised green pea-stem segments, growth is initiated, and there is a parallel progressive labeling of the RNA extracted by cold phenol. The bulk of the label is found in the 4S fraction. This fraction is more resistant to degradation by ribonuclease than a similar fractian obtained from tissue not treated with indoleacetic acid.     

吲哚乙酸对周丛生物去除水体中氮磷的影响及机理

为探究吲哚乙酸添加的先后顺序和质量浓度对周丛生物去除水体中氮磷的影响及其作用机理,以人工培养的周丛生物为研究对象,分别用预先经过吲哚乙酸培养的周丛生物(预处理组)和未经过吲哚乙酸培养的周丛生物(对照组)在含有不同质量浓度(0、5和10 mg·L-1)吲哚乙酸的氮磷污水中培养.结果 表明,添加吲哚乙酸提高了周丛生物对水体...     

气液色谱法测定水稻幼苗内源激素含量

研究了从水稻幼苗中提取４种激素（ＩＡＡ、ＡＢＡ、ＧＡ３、ＧＡ４）的样品处理方法、气液色谱法测定这４种激素的条件，实际分析了植物样品，该方法快速简便     

不同埋土深度杉木无性系萌蘖及内源激素含量的差异

以杉木‘洋020’扦插苗和‘洋020’组培苗为实验材料,通过盆栽实验,在调查不同埋土深度处理无性系萌蘖情况的基础上,应用酶联免疫吸附法测定了杉木无性系萌蘖高峰期枝叶、基部韧皮部、根尖等不同器官吲哚乙酸、赤霉素、细胞分裂素及脱落酸含量差异。结果表明：组培苗萌蘖能力强于扦插苗;随着埋土深度增加,2个无性系苗萌蘖能力均呈降低的趋势。不同埋土深度处理根尖和枝叶内源激素含量较高;随着培养时间延长,无性系枝叶吲哚乙酸含量呈上升趋势,赤霉素含量呈下降趋势;埋土深度6 cm处理有利于枝叶及根尖脱落酸的积累,埋土深度9 cm处理有利于根尖细胞分裂素的积累;无性系萌蘖与埋土深度呈极显著负相关,与赤霉素含量呈极显著正相关。不同埋土深度对杉木无性萌蘖有一定的影响,杉木无性系萌蘖高峰期均在8月份;不同器官植物内源激素含量是影响埋土深度处理杉木无性系萌蘖的主要影响因子之一。     

施肥及生长调节剂对烟草烟碱和钾含量的影响

本文研究了烟草品种、不同形态的氮肥以及生长调节剂 (IAA ,丙二酸 )对烟草烟碱和钾含量的影响。研究结果表明 ,品种间烟碱和钾含量存在明显差异 ,其中烟碱含量以中烟 90和中烟 98较低 ,钾含量以中烟 90和RG11较高 ;不同形态的氮肥对烟碱和钾含量的影响不同 ,施用硝酸钾烟碱积累最低 ,施用硝铵有利于烟叶钾的吸收 ;IAA和丙二酸都可以降低烟碱的含量 ,尤其IAA效果最明显。IAA明显提高烟叶的含钾量 ,而丙二酸则有降低烟叶含钾量的趋势     

上海地区大豆细菌性"叶斑病"病原研究

从上海地区的大豆叶斑病病株和病种上分离出20个细菌菌株,经菌体形态、培养性状、生理生化、致病性、Biolog和脂肪酸测定,并与12个国际标准菌株比较,在国内首次明确了大豆"叶斑病"是由大豆细菌性疫病菌Pseudomonas savastanoi pv.Glycinea(萨氏假单胞菌大豆致病变种)引起。种子可带菌和传病,10份自然病种的平均种子带菌率在7.6%,传病率在0.48%。     

四季鹅抱窝期间血浆某些代谢产物浓度的变化

四季鹅抱窝期间血浆葡萄糖浓度显著低于醒抱后恢复期水平,而血浆游离脂肪酸浓度高出四倍;并且两者呈强直线负相关。血浆总脂水平抱窝期显著高于醒抱后恢复期。结果提示:四季鹅在其抱窝期间,由于采食抑制,主要依靠消耗体内脂肪储备来满足其能量需要。     

Auxin in Coleus Stems: Limitation of Transport at Higher Concentrations

Previous indirect evidence that the endogenous concentration of plant hormones of the auxin type is controlled by a limitation of transport over the physiological range of concentration was confirmed by measuring directly the transport of the native auxin, indoleacetic acid, through segments of Coleus stems.     

芜菁花叶病毒对不结球白菜内源激素含量及代谢相关基因转录水平的影响

以不结球白菜感病品种‘矮脚黄’为材料,采用ELISA和Real-time PCR技术研究了芜菁花叶病毒(TuMV)侵染后不结球白菜内源生长素(IAA)、赤霉素(GA3)、脱落酸(ABA)和茉莉酸(JA)的动态变化以及内源激素相关基因的表达差异。结果表明:TuMV侵染后,植株发病症状的严重程度与IAA含量呈正相关;光敏色素相关蛋白基因PAP1对IAA的合成起负调控作用。处理植株体内GA3含量及其代谢相关基因,即原表皮因子基因PDF1表达量均明显低于对照植株,而ABA含量则随着接种时间的延长不断积累,始终高于对照植株。且发病症状越严重的植株中,GA3含量越低,ABA含量越高。处理植株JA含量和JA诱导基因,即营养贮藏蛋白基因Vsp1表达量在接种后均出现2次上升,且植株病症越严重,JA含量越低。TuMV侵染后,植株中IAA/ABA和GA3/ABA的值则明显低于对照植株,表明TuMV的侵染破坏了内源激素的平衡,干扰了植物的正常生长发育。     

内源激素含量变化对大葱抽薹的影响

为了探讨内源激素与大葱抽薹的关系,采用酶联免疫吸附法（ELISA）测定了大葱抽薹植株与未抽薹植株生长过程中脱落酸（ABA）、生长素（IAA）、赤霉素（GA3）以及玉米素核苷（ZR）内源激素的含量。结果表明：较高水平的ABA、IAA、zR含量以及较低水平的GA3含量对大葱抽薹具有促进作用;4种内源激索含量越冬前低、越冬后高,有利于大葱抽薹。     

Enhancement by Inositol of the Nodulation of Isolated Bean Roots

The percentage of isolated bean roots nodulated and the number of nodules per root were increased by the addition of mesoinositol to the agar medium into which the bases of bean roots were inserted. The following were without marked effect: glycine, thiamine, pyrodoxine, niacin, indoleacetic acid, gibberellic acid, kinetin, adenine, adenosinetriphosphate, biotin, riboflavin, calcium pantothenate, and folic acid.     

Hormonal regulation of growth in unfertilized cotton ovules

Exogenous plant growth regulators can substitute for pollination, fertilization, and subsequent embryo development in cotton. Isolated, unfertilized, immature ovules enlarge in the presence of kinetin, and both enlarge and produce fibers in the presence of indoleacetic acid or gibberellic acid or both. An extract of germinating cotton pollen qualitatively mimics the effect of exogenous hormones.     

细叶百合中内源激素的变化

应用ELISA测定了细叶百合鳞茎中的吲哚乙酸(IAA)、赤霉素(GA)、脱落酸(ABA)的含量。结果表明,不同年龄、不同部位鳞片中三种激素含量均有较大差异：随着年龄的增加,IAA的含量呈上升趋势,ABA含量呈下降趋势;外层鳞片和鳞片下层IAA含量最高,ABA含量最少,且具有较高的IAA／ABA和GA／ABA值。