宿主植物对AM真菌生长发育的影响

研究了宿主植物对丛枝菌根(Arbuscular mycorrhizae,AM)真菌(Glomus mosseae)生长发育的影响。结果表明:温室条件下,4种宿主植物都能与丛枝菌根真菌共生,综合比较菌根长度、根外菌丝量及孢子数3项指标,高粱敖杂1号对真菌的生长发育最为有利。宿主植物菌根长度及根中的可溶性糖质量分数与根外孢子数呈显著正相关,而宿主植物中磷质量分数与菌根真菌的生长发育没有显著的相关性。说明不同宿主植物甚至同种宿主植物不同基因型的品种与丛枝菌根真菌的共生状况不同,宿主植物的菌根长度及根中可溶性糖质量分数对菌根真菌的生长发育有显著影响。     

Influence of Soil Water Regimes on VA Mycorrhiza

A greenhouse trial was conducted with cassava inoculated with seven different VAM fungal species, i.e. Acaulospora longula, A. myriocarpa, Entrophospora colombiana, Glomus fasciculatum, G. manihotis, G. occultum and Scutellospora heterogama. A wet and a dry soil water regime was established to study the effect of water stress on performance of these fungi with a drought tolerant cassava clone. At harvest, N, P and K concentration was analysed in leaves, petioles, stems and roots.
In unstressed plants, five endophytes were effective whereas in stressed plants only three fungi improved growth beyond the non-mycorrhizal control. These were E. colombiana, G. manihotis and G. occultum which were most effective for P uptake under stress. Differential effects of VAM fungal species on root length and K. uptake were found. A. myriocarpa caused the greatest increase in length of feeder roots and G. occultum was one of the most effective for K uptake under both water regimes even though these fungi were not the most effective for dry matter production. Under each water regime, the concentration of N, P and K in leaves, petioles, stems and roots was influenced by the fungal species. Mycorrhizal endophytes differed in tolerance to dry soil conditions as found by root infection ratings. From the results it can be concluded that in addition to the improved P nutrition the enhanced root length and K uptake by VAM fungi may be important for drought tolerance of cassava.     

Effects of High Soil Temperature and Water Stresses on Malian Pearl Millet and Sorghum during Seedling Stage

Pearl millet ( Pennisetum americanum [L.] Leeke) and sorghum ( Sorghum bicolor [L.] Moench) can suffer from poor stand establishment under conditions of high soil temperature and water stresses. A line source sprinkler irrigation system and a charcoal pit technique were used to evaluate stand establishment and high correlations existed between the two methods. It may be possible to use the charcoal pit technique as a predictive tool in drought resistance breeding programs.     

丛枝菌根真菌对旱稻根际Pb形态分布的影响

为了研究丛枝菌根真菌对旱稻根际土壤中重金属铅(Pb)化学赋存形态的影响,采用盆栽实验的方法,研究了不同Pb处理水平(0,300,600 mg/kg)下,接种丛枝菌根真菌(AMF) Glomus mosseae对旱稻(Oryzal Sativa L.)根际Pb形态分布的影响。结果表明,接种处理下,旱稻根系侵染率以及根外菌丝量随着土壤Pb含量增加而显著降低（P<0.05）。与未接种处理相比,接种处理显著提高Pb处理下根际pH;300 mg/kg Pb处理下,接种显著增加土壤球囊霉素含量,600 mg/kg Pb处理下,接种处理显著降低土壤球囊霉素含量（P<0.05）;在Pb处理下,与未接种处理相比,接种处理显著提高可交换态Pb含量,显著降低了碳酸盐结合态以及铁锰氧化物结合态Pb含量,却显著增加了土壤中有机质结合态Pb含量（P<0.05）。这说明接种丛枝菌根真菌可以改变旱稻根际土壤中Pb的形态分布。     

Comparative Effects of Salt and Water Stress on Seed Germination and Early Embryo Growth in Two Cultivars of Sweet Sorghum

In semiarid regions of the Mediterranean basin, water and salinity stresses restrict crop establishment. The effects of salt and water stress on seed germination and early embryo growth (radicle and shoot growth) were investigated in laboratory in two cultivars of sweet sorghum [Sorghum bicolor (L.) Moench] – cv. ‘90‐5‐2′ and cv. ‘Keller’ – to verify how these stresses may limit crop growth during the very early stages of growing season. Six water potentials (ψ) of the imbibition solution (from 0 to ?1.0 MPa) in NaCl or polyethylene glycol (PEG) for salt and water stress tests, respectively, were studied. Daily germination was recorded, and radicle and shoot lengths and dry weights (DWs) were measured 2 days after initial germination. Seed germination was reduced (8–30% lower than control) by water stress at ψ 相似文献   

矿区复垦土壤接种丛枝菌根对玉米生长及营养吸收的影响

采用盆栽试验方法,研究了不同培养基质条件(10cm土5cm尾矿、8cm土7cm尾矿、5cm土10cm尾矿)下,接种丛枝菌根真菌和不接种丛枝菌根真菌(对照)两种处理对玉米生物量及植株中营养元素吸收的影响。结果表明,无论接种与否,植株的生物量都随覆土厚度增大而增大;与非菌根植物相比,接种菌根真菌的植株可以吸收更多的营养元素,而向地上部运移的Na较少,抑制了植株积累过量的Na盐;地上部Zn和Cu的含量受接种菌根的影响不大,而接种菌根降低了地上部Mn的含量。植株生物量及其植株体内的营养元素含量,接种菌根真菌后,覆土少的(覆5cm厚的土壤于10cm厚的尾矿上)相应高于不接种且覆土厚(覆10cm厚的土壤于5cm厚的尾矿上)的,说明接种菌根可以抵消由于覆土少而导致的植株生物量降低,并且对尾矿充填复垦土壤的植被重建具有重要作用。     

The Influence of Johnsongrass Infestation and Nitrogen Rates on Grain Sorghum

Gram sorghum [ Sorghum bicolor (L.) Moench] is grown on marginal land and johnsongrass [Sorghum haiepense (L.) Pers] is the most common and hard to control weed in this crop. The agronomic performance of gram sorghum at different nitrogen (N) and johnsongrass infestation levels in the field has not been adequately investigated. Therefore, research was conducted on a Decatur silty clay loam soil (Rhodic Paleudult) to determine the influence of N rates (0, 40, 80 and 120 kg ha^-1) and johnsongrass infestation levels (0, 2, 4, 6 and 8 plants per 5 m crop row) on the growth and yield of grain sorghum (cv. GK522G) and johnsongrass. Increase in N rates increased seed weight, seed yield and percent protein in grain sorghum as well as dry matter and percent protein of johnsongrass. The increase in johnsongrass infestation (from 0 to 8 plants per 5 m crop row) significantly decreased the performance of grain sorghum and johnsongrass at all the N rates. The results suggest that 40 kg N ha^-1 with up to 2 johnsongrass plants per 5 m crop row or 80 kg N ha^-1 with up to 4 johnsongrass plants per 5 m crop row produced grain sorghum yields equivalent to absence of johnsongrass in the crop.     

Einfluß von Form und Plazierung der N- und P-Düngung auf Wurzelentwicklung, Phosphat-Aufnahme und Wachstum von Sonnenblumen (Helianthus annuus) und Zuckerhirsen (Sorghum bicolor)

Influence of form and placement of N- and P-fertilisation on the root development, the P-uptake, and the growth of sunflower ( Helianthus annum ) and sweet sorghum ( Sorghum bicolor ).
In pot- and rhizotrone-experiments with Sorghum bicolor and Helianthus annuus , a higher P-uptake was achieved by placement of diammonphosphate (DAP) than by either placement of triplesuperphosphate (TSP) or by broadcasting DAP or TSP, respectively. Dual placement of TSP and ammonia increased the P uptake but it was less effective than placement of DAP for sunflowers, but not for sweet sorghum. Placement of nitrogen resulted in a local root accumulation, where ammonia had a greater effect than nitrate for both of the species. Single placement of TSP had no effect on either P uptake or root development.
Thus, the higher P uptake at DAP placement could be attributed to three main factors: the local root accumulation caused by the placement of nitrogen, a specific physiological ammonia-effect, and the lower binding intensity of the phosphate in the DAP-band compared with placed TSP (W erner and S trasser 1993). The improvement in spatial availability caused by the NH₄⁺ -induced root accumulation was of a greater importance for the P-uptake than was the improvement of chemical availability in the DAP-band. The better response of sunflowers in comparison with sweet sorghum was attributed to better acquisition ability in sweet sorghum.     

Comparison of Drought Tolerance of Maize,Sweet Sorghum and Sorghum‐Sudangrass Hybrids

In drought‐prone environments, sweet sorghum and sorghum‐sudangrass hybrids are considered worthy alternatives to maize for biogas production. The biomass productivity of the three crops was compared by growing them side‐by‐side in a rain‐out shelter under different levels of plant available soil water (PASW) during the growing periods of 2008 to 2010 at Braunschweig, Germany. All crops were established under high levels of soil water. Thereafter, the crops either remained at the wet level (60–80 % PASW) or were subjected to moderate (40–50 % PASW) and severe drought stress (15–25 % PASW). While the above‐ground dry weight (ADW) of sweet sorghum and maize was insignificantly different under well‐watered conditions, sweet sorghum under severe drought stress produced 27 % more ADW than maize. The ADW of sorghum‐sudangrass hybrids significantly lagged behind sweet sorghum at all levels of water supply. The three crops differed markedly in their susceptibility to water shortage. Severe drought stress reduced the ADW of maize by 51 %, but only by 37 % for sweet sorghum and 35 % for sorghum‐sudangrass hybrids. The post‐harvest root dry weight (RDW) in the 0–100 cm soil layer for maize, sweet sorghum and sorghum‐sudangrass hybrids averaged 4.4, 6.1 and 2.9 t ha^?1 under wet and 1.9, 5.7 and 2.4 t ha^?1 under severe drought stress. Under these most dry conditions, the sorghum crops had relatively higher RDW and root length density (RLD) in the deeper soil layers than maize. The subsoil RDW proportion (20–100 vs. 0–20 cm) for maize, sweet sorghum and sorghum‐sudangrass hybrids amounted to 6 %, 10 % and 20 %. The higher ADM of sweet sorghum compared with maize under dry conditions is most likely attributable to the deep root penetration and high proportion of roots in the subsoil, which confers the sorghum crop a high water uptake capacity.     

Response of Root Branching and Shoot Water Potentials of French Beans (Phaseolus vulgaris L.) to Soil Moisture and Fertilizer Potassium

Extensive branching patterns of roots and the maintenance of adequate water within shoots enables plants to overcome water stress. However, information on the relationship between fertilizer potassium, root branching patterns and shoot water potentials of food legumes grown under different soil moisture regimes is scarce. Thus, an experiment was conducted in a phytotron to ascertain the effect of fertilizer potassium on root branching patterns and shoot water potentials of a popular tropical food legumes (Frenchbeans Phaseolus vulgaris L). The plants were grown in a sand medium with 0.1, 0.8 or 3.0 mM of potassium under a suboptimal and optima) soil moisture regime.
Root lengths and dry weights were enhanced by potassium, especially under a suboptimal soil moisture regime. The branching patterns changed due to potassium, where the numbers of second and third order roots increased under both soil moisture regimes, although the impact was greater in plants grown with low soil moisture. Plant water contents measured in terms of shoot water potential, relative water contents, rurgid weight: dry weight ratio and water uptake capacity were also increased by potassium. A positive relationship was observed between root branching patterns and water potentials with increasing potassium levels especially in plants grown under suboptimal soil moisture conditions. Shoot growth and nodulation was also promoted by potassium. The ability of plants to develop a more extensive branching pattern of roots by inducing a greater number of second and third order roots and changing the root branching habit from a herringbone to a dichotomous type to maintain a greater shoot water potential especially under low soil moisture regimes is presented.     

Effects of free‐air CO2 enrichment and drought on root growth of field grown maize and sorghum

Increasing CO₂ concentration ([CO₂]) is thought to induce climate change and thereby increase air temperatures and the risk of drought stress, the latter impairing crop growth. The objective of this study was to investigate the effects of elevated [CO₂] and drought stress on root growth of one maize genotype (Zea mays cv. Simao) and two sorghum genotypes (Sorghum bicolor cv. Bulldozer and Sorghum bicolor × Sorghum sudanense cv. Inka) under the cool moderate climate of Central Europe. It was hypothesized that root growth stimulation due to elevated [CO₂] compensates for a reduced root growth under drought stress. Therefore, we established an experiment within a f ree‐a ir c arbon dioxide e nrichment system (FACE) in 2010 and 2011. Sorghum and maize genotypes were grown under ambient [CO₂] (385 ppm CO₂) and elevated [CO₂] (600 ppm CO₂) and in combination with restricted and sufficient water supply. Elevated [CO₂] decreased root length density (RLD) in the upper soil layers for all genotypes, but increased it in deeper layers. Higher [CO₂] enhanced specific root length (SRL) of “Simao” and “Bulldozer,” however, did not affect that of “Inka.” “Simao” achieved a higher SRL than the sorghum genotypes, indicating an efficient investment in root dry matter. Although elevated [CO₂] affected the root growth, no interaction with the water treatment and, consequently, no compensatory effect of elevated [CO₂] could be identified.     

Seed Size and Water Potential Effects on Water Uptake, Germination and Growth of Lentil

Seed size is an important parameter for plant growth and yield. The effects of seed size and water potential on seed water uptake, germination and early growth of lentil ( Lens culinaris Medik. cvs. Jor-1 and Jor-2) were investigated. Rate of water uptake by seed size (small, medium and large) from solutions containing different water potentials (0, –0.5 and –1.0 MPa, as polyethylene gly-col-8000) was higher in large than in medium or small seeds of both cultivars, regardless of water potential. Rate of water taken into seeds was higher in Jor-2 than in Jor-1. Decreasing water potential (more stress) had adverse effects on rate of water uptake by seeds in both cultivars. In another experiment, with lentils grown in a greenhouse at different soil metric potentials (–0.03, –0.15 and –0.30 MPa), seed size or cultivar had no effects on germination percentage (GP), but GP was reduced as soil water potential decreased (more stress), in greenhouse soil, shoot dry matter (SDM), root dry matter (RDM), plant height, total root length (TRL) and number of primary branches per plant of 35-day-old plants from large seeds were larger than those of plants from medium and small seeds of both cultivars. Increasing soil water deficit progressively decreased each of these traits. Plants from large seeds had higher SDM, RDM and TRL than those from small seeds at intermediate soil water potential (–0.15 MPa) in comparison with the control (–0.03 MPa) or severe (–0.30 MPa) soil water potentials. Larger seeds produced larger plants than smaller seeds, and this appeared to be more pronounced under intermediate than well-watered or more severe water-stressed conditions. Faster early growth of plants from larger seeds may be advantageous in establishing plants under semiarid conditions.     

Osmotically Primed Seed and Seedling Reactions to Variations in Day/Night Temperature

The effects of alternating day/night temperatures, varying in maxima and minima but all averaging 30 °C, on germination and seedling characteristics of Sorghum bicolor L. (Moench) were investigated. Seeds of sorghum CSV 15 were soaked in 2, 4 or 6 g NaCl l⁻¹ solutions for 2 days at 25 °C, soaked in water for 1 day at 25 °C, or untreated. After treatment, drying and storage, seeds were germinated at 30/30 (day/night), 35/25, 40/20 or 41/19 °C temperature regimes under a polyethylene glycol-induced drought level of −3 bar. Results revealed no advancement of germination percentage but a partial increase in germination speed by osmotic seed treatments. All three osmotic treatments also reduced the plumule/radicle ratio owing to a speculated increase in root over shoot growth. Temperature regimes significantly influenced both germination and seedling characteristics with the optimum temperature appearing to be 35/25 °C. Increasing the temperature amplitude from 30/30 to 35/25 °C increased germination speed, which was reduced by incremental increases in temperature amplitude to 40/20 or 41/19 °C.     

高粱原生质体的制备及转化方法研究

以高粱幼苗为材料,利用1%的纤维素酶Cellulose R10和0.75%的果胶酶Macerozyme R10处理高粱幼茎,对比不同的酶解时间,发现酶解7h原生质体浓度最高、质量最佳。以经过氯化铯密度梯度离心法获得的高纯度质粒对获得的原生质体进行转化,用激光共聚焦进行荧光观察,结果显示,GFP和YFP在全细胞表达。本研究首次建立了高粱以高粱幼茎为材料的原生质体制备及转化体系,其方法简单、高效,可以用于高粱基因功能研究。     

Effects of water and nitrogen management on fibrous root distribution and turnover in sugar beet

Two field trials were carried out in two years in heavy soils of NE Italy, with the aim of studying the effects of water and nitrogen management on fibrous root distribution and dynamics in sugar beet (cv. Dorotea). In conditions of moderate water deficit (year 2002, Conselice, Ravenna, clay soil), two water regimes (irrigation to 100% of potential evapotranspiration, and rainfed) were factorially combined with three rates of nitrogen application (180, 90, 0 kg ha⁻¹). Irrigation increased volumetric root length density (RLD_v) without N application and at the medium N rate – a common amount in beet cultivation – but reduced it at the maximum N dose. The medium N rate increased RLD_v and shifted root distribution towards shallow layers, regardless of water regime.In the conditions of marked drought of 2003 (Legnaro, Padova, silty-loam soil), at a single rate of N supply (90 kg ha⁻¹) irrigation increased total production (length) of fibrous roots throughout the soil profile (1.8 m), except in the 0.5–1 m interval, and improved the length of standing living roots during the season. Although the maximum root depth at the end of the season was similar in the two water regimes (about 1.9 m), irrigated roots reached the saturated soil layers 10 days earlier than in rainfed plants. The main result was reduced root turnover in deep soil layers (>1 m) and an increase at the surface in the rainfed treatments in conditions of drought, a probable mechanism of adaptation to a more marked gradient of soil moisture compared with irrigation.     

Response of Sorghum to Abiotic Stresses: A Review

Sorghum [(Sorghum bicolor L.) Moench] is a highly productive crop plant, which can be used for alternative energy resource, human food, livestock feed or industrial purposes. The biomass of sorghum can be utilized as solid fuel via thermochemical routes or as a carbohydrate substrate via fermentation processes. The plant has a great adaptation potential to drought, high salinity and high temperature, which are important characteristics of genotypes growing in extreme environments. However, the climate change in the 21st century may bring about new challenges in the cultivated areas. In this review, we summarize the most recent literature about the responses of sorghum to the most important abiotic stresses: nutrient deficiency, aluminium stress, drought, high salinity, waterlogging or temperature stress the plants have to cope with during cultivation. The advanced molecular and system biological tools provide new opportunities for breeders to select stress‐tolerant and high‐yielding cultivars.     

不同倍性冬小麦根系生长对水分和密度的响应

为探讨不同倍性冬小麦根系生长对水分和密度的响应,利用3个倍性小麦材料（二倍体栽培一粒、四倍体栽培二粒、六倍体现代品种‘长武134’）通过在不同水分条件下进行密度试验,研究其拔节期和开花期的根系生长和水分消耗特征,结果表明,在拔节期密度的增加显著提高了小麦根系干物质累积量和根长总量,而竞争加剧使得高密度处理的根量优势到花期显著减弱;拔节期四倍体和六倍体的根重和根长均表现出高于二倍体的趋势,花期各倍性材料之间的根重无显著差异,但是高倍性材料仍保持显著的根长优势;干旱和高密度处理均减少了花期根系在表层的分布,增加了深层根系的分布量,利于深层水分的利用。最终得到结论,六倍体现代小麦根系在不同生育期的根系干物质分配更利于产量增长,而在水分亏缺地区构建合理的群体有益于根系下扎,从而促进土壤深层水分的利用,并提升产量。     

Root and Shoot Development of Phaseolus vulgaris L. (French Beans) as Affected by Soil Moisture and Fertilizer Potassium

The development of an extensive root system enables plants to overcome water stress. However, there is little information on the response of food legumes to soil moisture, especially during early growth, which determines crop establishment. Thus, an experiment was conducted under controlled conditions to identify the effect of soil moisture and fertilizer potassium on root and shoot growth of french beans ( Phaseolus vulgaris L.) seedlings. The seedlings were grown in a sand medium under a high and low soil moisture regime and with 0.1, 0.8 or 3.0 mM potassium.
Root lengths, dry weights and numbers of root hairs were greater under low soil moisture conditions. Potassium increased root growth irrespective of soil moisture regimes. The impact of potassium on root length was more pronounced under a high soil moisture regime. In contrast, potassium increased root dry weights and root hairs to a greater extent when plants were grown under dry conditions. The lack of adequate soil moisture increased specific leaf weights, and this phenomenon was reduced by the application of potassium. Shoot:root ratios also showed a similar phenomenon. The development of an extensive root system by french bean seedlings under dry conditions to extract a greater quantity of available soil moisture fur establishment and plant growth and the ability of potassium to promote this phenomenon is presented in this study.     

不同灌水量对酿造高粱生长、产量和水分利用率的影响

为了探讨不同灌水条件下酿造高粱[Sorghum bicolor (L.) Moench]需水规律和水分利用效率,在田间试验条件下,以布置在高粱冠层顶部直径为20 cm标准蒸发皿水面蒸发量为基础确定灌溉水量,设置不灌水（对照）、30%蒸发量、60%蒸发量和90%蒸发量4个灌水量,采用滴灌方法,研究不同灌水量对酿造高粱生长、产量和水分利用效率的影响。结果表明,在开花期,90%蒸发量灌溉处理高粱植株株高、茎粗和叶叶面积均明显高于对照处理。90%蒸发量灌溉处理高粱生物产量比60%蒸发量、30%蒸发量和对照处理分别增加9.19%、20.92%和17.27%;高粱籽粒产量分别比60%蒸发量、30%蒸发量和对照处理分别增加8.99%、16.89%和19.74%。90%蒸发量、60%蒸发量和30%蒸发量灌溉处理比不灌溉处理千粒重分别提高14.71%、10.20%和7.89%。30%蒸发量、60%蒸发量、90%蒸发量灌溉处理的全生育期耗水量比不灌溉处理分别增加15.2%、32.9%和44.8%。不同灌溉处理日均耗水量高峰值均出现在大喇叭口期-扬花期。不同灌溉条件下的高粱需水量在400~590 mm之间。随着灌溉量增加,高粱耗水量和籽粒产量相应增加,而水分利用效率呈现出降低趋势,90%蒸发量、60%蒸发量、30%蒸发量灌溉处理比不灌溉处理的水分利用效率分别下降17.3%、17.3%和11.1%。     

Effect of Rooting Pattern on Rice Productivity under Different Water Regimes

Three rice varieties, viz. ADT 31 (drought tolerant) CO 41 (upland) and IR 50 (drought susceptible), were employed to study their rooting patterns under different water regimes. ADT 31 had better root penetration and distribution under water stress situation compared to others. The values on root weight also established a similar trend. Isotopic studies with ³²P also provided evidence in this regard wherein the variety ADT 31 showed its adaptiveness to drought. Marginal reduction in plant height, less tiller production and leaf area reductions were noted in ADT 31 and CO 41 under decreasing soil moisture which showed its ability to cope with the water stress situation. Though IR 50 recorded higher grain yield at normal irrigation, a drastic reduction was noted under extreme water deficit. But ADT 31 recorded consistent values in all situations with slight marginal reduction which evidently proved the interdependent nature of rooting pattern and its influence on productivity.