Status and distribution of soil sulphur fractions,total nitrogen and organic carbon in camp and non-camp soils of grazed pastures supplied with long-term superphosphate

Summary Topsoils (0–75 mm) from four different soil types were collected from stock camp and non-camp (main grazing area) areas of grazed pastures in New Zealand, which had been fertilised annually with superphosphate for more than 15 years, in order to assess the effects of grazing animals on the status and distribution of soil S fractions and organic matter. These soils were analysed for organic C, total N, total S, C-bonded S, hydriodic acid-reducible S, 0.01 M CaCl₂, and 0.04 M Ca(H₂PO₄)₂-extractable S fractions, and soil pH. Soil inorganic and organic S fractions extracted by NaHCO₃ and NaOH extractants were also determined. The results obtained showed that camp soils contain higher soil pH, organic C, total N, total S, organic (C-bonded S and hydriodic acid-reducible S) and inorganic S fractions, NaHCO₃-and NaOH-extractable soil S fractions but a lower anion retention capacity than non-camp soils, attributed to a higher return of plant litter and animal excreta to camp soils. In both soils, total S, organic S, C-bonded S, and hydriodic acid-reducible S were significantly correlated with organic C (r0.90***, ***P0.001) and total N (r0.95***), suggesting that C, N, and S are integral components of soil organic matter. However, C: N : S ratios tended to be lower in camp (60: 5.6: 1–103: 7.2: 1) than in non-camp soils (60:6.1:1–117:8.3:1). Most (>95%) of the total soil S in camp and non-camp soils is present as organic S, while the remainder is readily soluble and adsorbed S (i.e. Ca(H₂PO₄)₂-extractable S). C-bonded S and hydriodic acid-reducible S constituted 55%–74% and 26%–45% of total S, respectively, reflecting a regular return of plant litter and animal excreta to the grazed pastures. NaHCO₃, and especially NaOH, extracted significantly higher amounts of total soil S (13%–22% and 49%–75%, respectively) than Ca(H₂PO₄)₂ or CaCl₂ (<5%). In addition, NaHCO₃ and NaOH-extractable soil S fractions were significantly rorrelated with soil organic S (r0.94***), C-bonded S (r0.90***) and hydriodic acid-reducible soil S (r0.93***). Differences between soils in either camp or non-camp areas were related to their sulphate retention capacities, as soils with high sulphate retention capacities (>45%) contain higher levels of C-bonded and hydriodic acid-reducible S fractions than those of low sulphate retention soils (<10%). Long-term annual superphosphate applications significantly increased the accumulation of soil organic and inorganic S fractions, and organic C and total N in the topsoil, although this accumulation did not occur when the superphosphate application rates were increased from 188 to 376 kg ha^-1 year^-1.     

Phosphorus in light fraction organic matter separated from soils receiving long-term applications of superphosphate

The objectives of this study were to: (1) characterize the P composition of light fraction (LF) organic matter in soils with different P inputs, and (2) determine if LF-organic P (Po) is a useful indicator of labile Po. Soils (0-7.5 cm depth) were obtained from a long-term (1952-1999) experiment set up to examine the effects of single superphosphate (0, 188 or 376 kg ha^-1 year^-1) on productivity of an irrigated pasture grazed by sheep. About half the mass of LF (separated by flotation on sodium iodide solution with specific gravity of 1.7) consisted of fine-grained mineral material. Inorganic P (Pi) associated with this mineral material comprised 25-40% of the total P in LF. The organic matter in LF (average C content of LF was ~25%) appeared to consist of two distinct parts: a mineral-associated component (stable, humic material) and free organic matter comprising recently deposited material. Organic matter adsorbed onto mineral surfaces represented up to 20% of LF-C and 30% of LF-N. The LF was less enriched in Po than C or N (the average Po concentration in LF was only 1.5 times that of whole soil, compared with a factor of 5.6 for C and 3.4 for N). The majority (about 70%) of the Po in LF was allocated to the stable, mineral-associated fraction. The free organic matter in LF was very low in Po and it had C:Po ratios (>500:1) which were much wider than those of whole soil organic matter (<~80:1). Decomposition of this material would be expected to result in net immobilization of P and, thus, it is unlikely to contribute a significant amount of P for plant growth.     

Long-term management impacts on soil carbon and nitrogen dynamics of grazed bermudagrass pastures

Managed pastures have potential for C and N sequestration in addition to providing forage for livestock. Our objectives were to investigate changes in soil organic C (SOC) and soil organic N (SON) concentrations and mineralizable C and N in cattle (Bos indicus) grazed bermudagrass [Cynodon dactylon (L.) Pers.] pastures up to 32 y after establishment. Management included low- and high-grazing intensity, fertilization, and winter overseeding with annual ryegrass (Lolium multiflorum Lam.) and clover (Trifolium sp.). Soil (0-15 cm) was sampled 7, 15, 26, and 32 y after establishment of Coastal and common bermudagrass pastures. No significant differences in SOC or SON concentrations were observed between Coastal and common bermudagrass pastures. Grazing strategies played important roles in C and N sequestration, as high-grazing intensity resulted in a lower increase in SOC and SON concentrations over time compared to low-grazing intensity. Increases in SOC were observed up to 26 y, while increases in SON were observed up to 32 y after establishment of bermudagrass pastures. Soil organic C increased 67 and 39% from 7 to 26 y at low-grazing intensity for bermudagrass+ryegrass and bermudagrass+clover pastures, respectively. SOC and SON concentrations did not increase beyond 15 y after bermudagrass establishment at high-grazing intensity. An exception was the Coastal bermudagrass+ryegrass pastures, which exhibited higher SON at 32 y than at 7 y at both grazing intensities. By 32 y, SON increased 83 and 45% in Coastal bermudagrass+ryegrass pastures at low- and high-grazing intensity, respectively, compared to 7 y. The introduction of clover to pastures decreased SOC and SON relative to ryegrass at high- but not at low-grazing intensity. Potentially mineralizable C increased from 7 to 15 y, while mineralizable N increased from 7 to 32 y. Potentially mineralizable N was also greater for bermudagrass+clover than bermudagrass+ryegrass pastures. Long-term increases in SOC and SON concentrations suggest that managed and grazed pastures have strong potential for C and N sequestration.     

温度对土壤吸附有机肥中可溶性有机碳、氮的影响

可溶性有机碳、氮(Soluble organic carbon or nitrogen,SOC和SON)可被土壤吸附.土壤可溶性有机碳、氮组分复杂,土壤对可溶性有机物吸附的不均一性会导致可溶性有机物组分的变化,大部分疏水性化合物被吸附,而亲水性化合物被释放进入溶液中[1].因此,可溶性有机碳、氮在土壤中的吸附,直接影响其在土壤-水系统中的迁移和行为[2-3].林地土壤中含有相当数量的可溶性有机养分,因此,关于林地土壤对可溶性有机养分的吸附特性,国外研究者已开展了不少研究.研究表明,可溶性有机碳吸附特性与土壤性质如pH、表面积、有机碳、铁铝氧化物和黏粒含量等因素有关[4-5].关于农业土壤对可溶性有机碳的吸附特性的影响,国内也开展了一些研究,主要集中在pH、铁铝氧化物含量等对吸附影响方面[6-9].     

Sulphur in soil and light fraction organic matter as influenced by long-term application of superphosphate

Numerous studies have examined the role of light fraction (LF) organic matter in soil C and N cycling, but there is no published information on the amounts and nature of S in LF. The objective of this work was to characterize the S composition of LF in soils receiving different inputs of fertilizer S. Soils (0-7.5 cm) were taken from a long-term experiment (1952-1999) set up to examine the effects of single superphosphate (SP) (applied at 0, 188, or 376 kg ha⁻¹ yr⁻¹, which equates to 0, 21, and 42 kg SO₄-S ha⁻¹ yr⁻¹) on the productivity of an irrigated, grass-clover pasture grazed by sheep. The S content of LF (separated by flotation on NaI solution with specific gravity 1.7) increased by ∼20-30% in response to SP. The LF was enriched in organic S compared with whole soil (S concentration in LF was ∼1000-1400 mg kg⁻¹ vs ∼400-500 mg kg⁻¹ in whole soil), but LF-S represented only 1.3-4.7% of soil S. Most (∼88%) of the S in LF was C-bonded, reflecting the dominance of this form of S in organic matter returned to the soil in dung and plant residues. Hydriodic acid (HI) reducible-S accounted for only ∼12% of LF-S, compared with 28-35% of whole soil organic S. Superphosphate tended to increase total soil N, due to improved clover growth. There was a strong positive relationship between total N and C-bonded S in whole soil and LF, whereas soil HI-S and N were not associated. Increases in C-bonded S where SP was applied appeared to be driven mainly by increases in soil N, which in turn were due to improved clover growth in response to phosphate supplied by SP. Increases in HI-S due to SP application were probably a direct response to inputs of S. As LF is a small pool of S, with a relatively wide C:S ratio (∼200:1), we concluded that it is unlikely to contribute a significant amount of plant-available S.     

Labile ester sulphate in organic matter extracted from podzolic soils

Summary We studied the effect of soil pretreatment, molecular-weight fractionation, and K₂SO₄ addition on the concentration and biochemical stability of ester sulphate in soil organic matter. A labile ester sulphate fraction (8.1 g S g^–1 soil) was detected in the organic matter extracted from a sulphate-rich podzolic sandy loam. This fraction was susceptible to loss during soil pretreatment with water and KCl solution and subsequent extraction of organic matter from the soil. The low-sulphate loam was low in labile ester sulphate (0.6 g S g^–1 soil) and the pretreatments had little effect. The addition of K₂SO₄ to the organic matter extracted from the low-sulphate soil resulted in the formation of appreciable amounts of labile ester sulphate. Newly formed ester sulphate tends to be biochemically less stable than indigenous ester sulphate in soil humic polymers and the ester sulphate associated with the low molecular-weight fractoin of soil organic matter appears to be more susceptible to loss by enzymatic hydroylsis. The results were interpreted in terms of steric effect. Ester sulphate groups bound to external surfaces of soil humic polymers may be easily accessible to sulphatase enzyme and thus readily mineralizable during incubation or extraction of soil organic matter at low soluble-sulphate levels. Sulphate groups on inner surfaces of the organic polymers are shielded from the enzyme due to size exclusion and hence more stable.     

Carbon and nitrogen mineralization of non-composted and composted municipal solid waste in sandy soils

A sterilized, but undecomposed, organic by-product of municipal waste processing was incubated in sandy soils to compare C and N mineralization with mature municipal waste compost. Waste products were added to two soils at rates of 17.9, 35.8, 71.6, and dry weight and incubated at for 90 d. Every 30 d, nitrate and ammonium concentrations were analyzed and C mineralization was measured as total CO₂-C evolved and added total organic C. Carbon mineralization of the undecomposed waste decreased over time, was directly related to application rate and soil nutrient status, and was significantly higher than C mineralization of the compost, in which C evolution was relatively unaffected across time, soils, and application rates. Carbon mineralization, measured as percentage C added by the wastes, also indicated no differences between composted waste treatments. However, mineralization as a percentage of C added in the undecomposed waste treatments was inversely related to application rate in the more productive soil, and no rate differences were observed in the highly degraded soil. Total inorganic N concentrations were much higher in the compost- and un-amended soils than in undecomposed waste treatments. Significant N immobilization occurred in all undecomposed waste treatments. Because C mineralization of the undecomposed waste was dependant on soil nutrient status and led to significant immobilization of N, this material appears to be best suited for highly degraded soils low in organic matter where restoration of vegetation adapted to nutrient poor soils is desired.     

Influence of soil compaction on carbon and nitrogen mineralization of soil organic matter and crop residues

 We studied the influence of soil compaction in a loamy sand soil on C and N mineralization and nitrification of soil organic matter and added crop residues. Samples of unamended soil, and soil amended with leek residues, at six bulk densities ranging from 1.2 to 1.6 Mg m^–3 and 75% field capacity, were incubated. In the unamended soil, bulk density within the range studied did not influence any measure of microbial activity significantly. A small (but insignificant) decrease in nitrification rate at the highest bulk density was the only evidence for possible effects of compaction on microbial activity. In the amended soil the amounts of mineralized N at the end of the incubation were equal at all bulk densities, but first-order N mineralization rates tended to increase with increasing compaction, although the increase was not significant. Nitrification in the amended soils was more affected by compaction, and NO₃ ^–-N contents after 3 weeks of incubation at bulk densities of 1.5 and 1.6 Mg m^–3 were significantly lower (by about 8% and 16% of total added N, respectively), than those of the less compacted treatments. The C mineralization rate was strongly depressed at a bulk density of 1.6 Mg m^–3, compared with the other treatments. The depression of C mineralization in compacted soils can lead to higher organic matter accumulation. Since N mineralization was not affected by compaction (within the range used here) the accumulated organic matter would have had higher C : N ratios than in the uncompacted soils, and hence would have been of a lower quality. In general, increasing soil compaction in this soil, starting at a bulk density of 1.5 Mg m^–3, will affect some microbially driven processes. Received: 10 June 1999     

长期施肥潮土在玉米季施肥初期的有机碳矿化过程研究

以黄淮海平原长期定位试验地2007年玉米播种期土壤为研究对象,通过室内37天的培养实验并选择4个应用比较广泛的方程对土壤有机碳矿化过程进行拟合,其目的主要是为了比较研究长期不同施肥土壤在玉米季施肥初期有机碳矿化过程及主要矿化参数的差异,并评估矿化参数和主要土壤性质之间的相关关系.结果表明,37天培养期内各施肥处理土壤CO2-C累积释放量与土壤有机碳、全氮含量和微生物活度均呈显著正相关,大小依次为OM＞1/2OM+1/2NPK＞NPK＞NP＞PK＞CK＞NK,有机碳矿化过程均呈曲线形式,与Jones(1984)改进的一级动力学方程拟合效果最好.拟合所得土壤潜在可矿化有机碳量(C0)、易矿化有机碳量(C1)和初始潜在矿化速率(C0k)均表现出有机肥处理高于化肥处理,施肥处理高于不施肥处理(NK处理除外),与土壤有机碳、全氮和土壤微生物活度均呈显著正相关;有机碳矿化速率(k)和土壤潜在可矿化有机碳占土壤总有机碳的比例在处理间差异均不显著,除k与有机碳呈显著负相关外,其他与土壤性质均无显著相关性.因此,我们推测有机肥和化肥的平衡施用均能显著增强土壤有机碳的矿化作用,有利于土壤无机养分的释放,同时使部分有机碳在土壤中积累.     

长期施用有机肥与化肥对潮土土壤化学及生物学性质的影响

研究长期施用有机肥与化肥对潮土土壤化学和生物学性质的影响结果表明,有机肥和化肥均使土壤有机质、全N、全P、速效磷、速度钾、阳离子交换性提高,增加土壤微生物数量和活性,但有机肥在培肥地力、创造有利于微生物生长繁育的土壤环境方面明显优于化学肥料。     

Biological activity and organic matter mineralization of soils amended with biowaste composts

This study aims to elucidate the significance of compost and soil characteristics for the biological activity of compost‐amended soils. Two agricultural soils (Ap horizon, loamy arable Orthic Luvisol and Ah horizon, sandy meadow Dystric Cambisol) and a humus‐free sandy mineral substrate were amended with two biowaste composts of different maturity in a controlled microcosm system for 18 months at 5 °C and 14 °C, respectively. Compost application increased the organic matter mineralization, the C_mic : C_org ratio, and the metabolic quotients significantly in all treatments. The total amount of C_org mineralized ranged from < 1 % (control plots) to 20 % (compost amended Dystric Cambisol). Incubation at 14 °C resulted in 2.7‐ to 4‐fold higher cumulative C_org mineralization compared to 5 °C. The C_mic : C_org ratios of the compost‐amended plots declined rapidly during the first 6 months and reached a similar range as the control plots at the end of the experiment. This effect may identify the compost‐derived microbial biomass as an easily degradable C source. Decreasing mineralization rates and metabolic quotients indicated a shift from a compost‐derived to a soil‐adapted microbial community. The C_org mineralization of the compost amended soils was mainly regulated by the compost maturity and the soil texture (higher activity in the sandy textured soils). The pattern of biological activity in the compost‐amended mineral substrate did not differ markedly from that of the compost‐amended agricultural soils, showing that the turnover of compost‐derived organic matter dominated the overall decay process in each soil. However, a priming effect occurring for the Dystric Cambisol indicated, that the effect of compost application may be soil specific.     

添加生物质炭对红壤水稻土有机碳矿化和微生物生物量的影响

通过室内培育试验,研究了添加生物质炭对江西红壤水稻土有机碳矿化和微生物生物量碳、氮含量的影响。结果表明:红壤有机碳矿化速率在培育第2天达最大值后迅速降低,培养7天后下降缓慢并趋于平稳;添加生物质炭降低了土壤有机碳的矿化速率和累积矿化量,培养结束时,不加生物质炭的对照处理中有机碳的累积矿化量分别比添加0.5%和1.0%生物质炭的处理高10.0%和10.8%。此外,生物质炭的加入显著提高了土壤微生物生物量,添加0.5%生物质炭处理的土壤微生物生物量碳、氮含量分别比对照高111.5%～250.6%和11.6%～97.6%,添加1.0%生物质炭处理的土壤微生物生物量碳、氮含量分别比对照高58.9%～243.6%和55.9%～110.4%。相同处理中,干旱的水分条件下(40%田间持水量)微生物生物量要高于湿润的水分条件(70%田间持水量)。同时,添加0.5%和1.0%的生物质炭使土壤代谢熵分别降低2.4%和26.8%,微生物商减少了43.7%和31.7%。     

添加牛粪对长期不同施肥潮土有机碳矿化的影响及激发效应

为了探讨长期不同施肥潮土有机碳矿化对添加牛粪的响应特征及添加牛粪对长期不同施肥潮土有机碳矿化的激发效应,以始建于1986年的长期定位试验为平台,通过室内恒温培养的方法研究添加等氮量牛粪后长期不同施肥(不施肥,CK;常量有机肥,SMA;常量化肥,SMF;常量有机无机配施,1/2(SMA+SMF))潮土有机碳矿化、土壤有机碳及活性碳库组分(微生物量碳、可溶性有机碳、颗粒有机碳和易氧化有机碳)含量的变化特征。结果表明:无论添加牛粪与否,长期不同施肥潮土有机碳矿化过程均符合一级动力学方程,而牛粪的添加显著增加了长期不施肥、长期单施化肥和长期有机无机配施土壤的有机碳矿化速率常数,增长幅度分别为21.74%、35.00%和45.00%;添加牛粪提高了长期不同施肥潮土有机碳、微生物量碳、颗粒有机碳和易氧化有机碳含量,却显著降低了可溶性有机碳含量;牛粪对长期不施肥、长期施用常量有机肥、常量化肥和常量有机无机配施潮土有机碳矿化的正激发效应分别达到了48.56%、3.60%、48.43%和3.92%,且对长期不施肥及长期施用常量化肥潮土的激发效应显著高于对长期施用常量有机肥及长期有机无机配施土壤;冗余分析显示添加牛粪对长期不同施肥土壤有机碳矿化的激发效应与土壤活性组分碳氮比呈正相关,与土壤养分含量呈负相关。该研究不仅为合理施用有机肥和实现农田生态系统的可持续发展提供理论依据,还有利于实现农业资源再利用及其效益最大化。     

长期施肥下褐土易氧化有机碳及有机碳库的变化特征

本研究探讨了24年长期施肥对褐土土壤有机碳(TOC)、有机碳储量(TOCs)、净固碳效率(NCSE)和碳库管理指数(CPMI)的影响,为评价褐土土壤碳库变化与质量及科学施肥提供理论依据。研究以褐土肥力与肥料长期定位试验为平台,通过9个处理[A组:不施肥处理(N_0P_0、CK);B组:单施无机肥处理(N_1P_1、N_2P_2、N_3P_3和N_4P_4);C组:有机肥与无机肥配施处理(N_2P_1M_1、N_3P_2M_3和N_4P_2M_2);D组:单施高量有机肥处理(M_6)]测定土壤TOC与易氧化有机碳(ROOC)含量,并计算TOCs、NCSE及CPMI等相关指标。结果表明,在不同土层不同时期施用较高量有机肥配施无机肥及施用高量有机肥(N_3P_2M_3、N_4P_2M_2和M_6)均可提高TOC和ROOC含量,且随土层深度加深提升作用减弱。TOCs、NCSE与0~20 cm土层TOC含量在时间和空间上的变化规律基本一致。施用高量有机肥(C组、D组)可有效提高TOCs,A组、B组的TOCs均值分别比C组、D组低76.77%与17.36%。长期施肥处理可提高NCSE,尤其是施用有机肥处理可显著提高NCSE。NCSE为D组C组A组=B组;D组NCSE为1 152.27 kg·hm~(-2)·a~(-1),是C组的2.51倍,B组的16.20倍。与试验前相比,C组和D组的CPMI无显著变化,且C组与D组间差异不显著,但A组与B组比试验前降低16.38~40.02。与A组(CK)相比,B组中N1P1处理与C、D组处理显著影响CPMI,提高了23.30~45.67。在0~40 cm土层CPMI与ROOC含量呈显著正相关,CPMI可以很好地指示有机碳的变化。可见,施用高量有机肥或者较高量有机肥与无机肥配施可极显著提高褐土土壤TOCs、NCSE和CPMI,即施用高量有机肥或者较高量有机肥与无机肥配施(N_3P_2M_3和N_4P_2M_2)有利于褐土有机碳的固存,可减少无机肥的施用量,使土壤性质向良性方向发展,培肥土壤。     

Carbon mineralization in soils of different textures as affected by water-soluble organic carbon extracted from composted dairy manure

The water-soluble organic C in composted manure contains a portion of labile C which can stimulate soil microbial activity. The objective of this experiment was to evaluate the effects of water-soluble organic C extracted from composted dairy manure on C mineralization in soil with different textures. Three soils with textures varying from 3 to 54% clay were amended with 0 to 80 mg water-soluble organic C kg^–1 soil extracted from a composted dairy manure and incubated for 16 weeks at 23°C. The total amount of C mineralized was greater than the amount of C added in the three soils. Differences in mineralizable C with and without added water-soluble organic C were approximately 13–16 times, 4.8–8 times, and 7.5–8 times greater than the amount of C added to clay, loam, and sand soils, respectively. The results of this experiment suggest that immediately following composted manure applications, C mineralization rates increase, and that most of the C mineralized comes mainly from the indigenous soil organic C pool.CLBRR contribution No. 94-71     

Changes in soil organic matter and net nitrogen mineralization in heathland soils,after removal,addition or replacement of litter from Erica tetralix or Molinia caerulea

Summary The effects of different litter input rates and of different types of litter on soil organic matter accumulation and net N mineralization were investigated in plant communities dominated by Erica tetralix L. or Molinia caerulea (L.) Moench. Plots in which the litter on the soil had repeatedly been removed were compared with plots in the same plant community in which litter had been added to the soil. In another treatment, litter was removed and replaced by litter from the other plant community. Net N mineralization was measured in situ after 5 years. Less soil organic matter and soil N was found in plots in which litter had been removed, compared with control plots, or plots to which litter had been added, but these differences were significant for the Erica sp. soils only. Plots in which litter had been replaced and control plots did not differ significantly in the amount of soil organic matter. However, in both plant communities, the differences agreed with the faster decomposition rate of Molinia sp. litter compared with Erica sp. litter. The gravimetric soil moisture content was correlated positively with the amount of soil organic matter, both in the Erica sp. soils and the Molinia sp. soils. Net N mineralization rates (g N m^-2) differed significantly between treatments for Erica sp. soils but no for Molinia sp. soils. For Erica sp. soils, net N mineralization rates increased with increasing amounts of soil organic matter and soil N. Replacing the litter with Molinia sp. litter (which differs in chemical composition) had no clear additional effect on the net N mineralization rate.     

Biochemical properties and biodegradation of dissolved organic matter from soils

Various biologically mediated processes are involved in the turnover of dissolved organic matter (DOM) in soil; however, relatively little is known about the dynamics of either the microbial community or the individual classes of organic molecules during the decomposition of DOM. We examined the net loss of DOC, the mineralisation of C to CO₂ and the degradation of DOC from six different soils by soil microorganisms. We also quantified the changes in the concentrations of protein, carbohydrate and amino acid C during microbial biodegradation. Over a 70-day incubation period at 20°C, the mineralisation of DOC to CO₂ was described by a double exponential model with a labile pool (half-life, 3–8 days) and a stable pool (half-life, 0.4–6 years). However, in nearly all cases, the mass loss of DOC exceeded the C released as CO₂ with significant deviations from the double exponential model. Comparison of mass DOC loss, CO₂ production and microbial cell counts, determined by epifluorescence microscopy, showed that a proportion of the lost DOC mass could be accounted for by microbial assimilation. Carbohydrate and protein C concentrations fluctuated throughout the incubation with a net change of between 3 to 13 and −30 to 22.4% initial DOC, respectively. No amino acid C was detected during the incubation period (level of detection, 0.01 mg C l⁻¹).     

长期施肥对红壤旱地土壤活性有机碳和酶活性的影响

以江西进贤长期肥料定位试验为平台,研究了红壤旱地不同施肥措施对土壤微生物生物量、活性有机C、C库管理指数以及土壤酶活性的影响。研究结果表明:与不施肥和单施化肥土壤相比,施有机肥处理土壤的pH、CEC、有机C、全N、全P、无机N、速效P、速效K及土壤微生物生物量均显著增加,土壤活性有机C和C库管理指数也较试前土壤和其他处理土壤明显提高,此外,土壤的转化酶、脱氢酶、脲酶和酸性磷酸酶活性也较其他处理显著增加。土壤微生物生物量、活性有机C以及4种土壤酶活性之间的相关关系显著,且它们均与土壤有机C、全N、全P、无机N、速效P等土壤养分呈显著正相关。因此,红壤旱地通过长期施用有机肥或与无机肥配施,不仅能显著提高土壤有机质的数量和质量,而且能增加土壤微生物生物量和酶活性,从而显著提高土壤肥力和土壤持续生产力。     

长期施肥下新疆灰漠土有机碳及作物产量演变

为明确长期不同施肥下新疆灰漠土有机碳和作物产量演变特征,依托始于1990年的灰漠土肥力长期定位监测试验,选择对照(CK,不施肥)、施氮磷肥(NP)、氮磷钾平衡施肥(NPK)、氮磷钾配合常量有机肥(NPKM)、氮磷钾配合高量有机肥(h NPKM,有机肥施用量为NPKM的2倍)、氮磷钾配合秸秆还田(NPKS)6个处理,分析不同处理下土壤有机碳和小麦、玉米产量演变特征,探讨碳投入及有机碳与作物产量的关系。结果表明:1)长期耗竭种植(CK)、连续施用NP或NPK肥,灰漠土有机碳含量持续下降,年均下降速率分别为0.094 g·kg~(-1)、0.043 g·kg~(-1)和0.053 g·kg~(-1),表明施化肥(NP、NPK)不能维持土壤有机碳含量,不利于土壤肥力的保持。NPKM和h NPKM处理,土壤有机碳显著增加,年均增加0.360 g·kg~(-1)和0.575 g·kg~(-1),增施有机肥是快速提高灰漠土肥力的重要措施。秸秆还田处理(NKPS),土壤有机碳年均增幅0.006 g·kg~(-1),与NPK处理对比,秸秆还田虽没有大幅度提高土壤有机碳,但维持了土壤肥力。2)较CK,长期化肥有机肥配施(NPKM、h NPKM)显著增加了作物产量(P0.05)。与NP和NPK比较,长期化肥有机肥配施显著提高了小麦产量(P0.05),但玉米产量与施化肥处理差异不显著(P0.05),玉米产量以平衡施肥(NPK)的增幅最高,达到220 kg·hm~(-2)·a~(-1)。小麦的产量变异系数(29.1%~43.9%)高于玉米产量变异(19.0%~32.7%)。化肥配合秸秆还田(NPKS)处理的小麦增产幅度与高量施用有机肥(h NPKM)处理接近,喻示了秸秆还田对作物增产的作用不可忽视。3)碳投入与土壤有机碳和小麦、玉米产量有显著线性正相关(P0.05)。基于以上分析,在干旱区灰漠土增加土壤碳投入(有机肥或秸秆)仍然是最基本的土壤培肥措施。     

The influence of American Chestnut (Castanea dentata) on nitrogen availability, organic matter and chemistry of silty and sandy loam soils

American chestnut trees once dominated vast areas of deciduous forest in eastern North America, but the exotic chestnut blight almost eliminated the species from the region. Introduction of blight-resistant American chestnut hybrids will probably start in the next decade after many years of tree breeding. What were the historic effects of chestnut on forest soils, and what changes may follow reintroduction of hybrid chestnuts? A site in southern Wisconsin provided an opportunity to examine the effect of chestnut trees on soil properties. At this site, 600 km northwest of chestnut's historic distribution, naturalized chestnuts have spread throughout an intact mixed-species forest from nine planted trees. The site contains soil developed on a silty loess-mantled ridge that abuts sandier hillslopes, allowing the effects of individual chestnuts to be examined on two soil types. I sampled and analyzed forest floor and mineral soils beneath canopies of individual American chestnuts and the surrounding mixed-species deciduous forest on fine-silt and sandy-loam soil types. On sandy loam soils, total soil carbon (C) and nitrogen (N), inorganic N and net mineralization and nitrification rates were 10–17% higher beneath chestnut canopies compared to soils beneath mixed-species deciduous forest. The pool of total soil N beneath chestnut canopies was positively related to the silt content of the sandy loam soils. In contrast, there were no differences between properties of chestnut canopy and mixed-species deciduous forest soils on the fine silt texture class. On sandy loam soil conditions common throughout the pre-blight distribution of American chestnut, soil biogeochemical processes differ beneath individual chestnut trees relative to a diverse mixture of deciduous species. These findings suggest that widespread chestnut reintroduction has the potential to alter both stand- and watershed-scale processes.