Effects of bovine cytochrome P450 single-nucleotide polymorphism, forage type and body condition on production traits in cattle

Relating single-nucleotide polymorphisms (SNP) to cows with acceptable productivity could benefit cattle breeders in areas where tall fescue is the predominant forage. This study aimed to (i) identify SNPs in bovine cytochrome P450 3A28 (CYP3A28) and (ii) determine the associations between SNP genotype, forage and cow body condition (BC). Genotype (CC, CG or GG) and forage [Kentucky-31 wild-type endophyte-infected tall fescue (KY+) vs. bermudagrass] effects on milk volume and quality were determined in Herd 1 cows (123 cows); in Herd 2 (99 cows), genotype and BC (low vs. moderate) effects on ovarian follicle size, calving date and calving per cent were determined; and in Herd 3 (114 cows), effects of genotype and fescue cultivar [KY+ vs. non-toxic endophyte-infected tall fescue (HiMag4)] were related to calving per cent, calving date and weaning weights of both cow and her calf. A cytosine (C) to guanine (G) transversion at base 994 (C994G) in CYP3A28 was identified. There was a genotype × forage type interaction (p < 0.05) on milk protein in Herd 1 cows; CC cows grazing bermudagrass had greater milk protein percentage in relation to other cows in the herd. In Herd 2, BC and genotype × BC tended (p < 0.10) to influence follicle size and Julian calving date respectively. Diameter of the largest follicle tended to be larger in moderate BC than in low-BC cows; whereas, CC and CG cows in moderate BC and homozygous (CC and GG) cows in low BC tended to calve 14 days earlier in relation to CG cows in low BC. In Herd 3, there was a genotype × forage type interaction (p < 0.05) on calving per cent, Julian calving date and calf weaning weight. In this study, genetic alterations (G allele at C994G) coupled with nutritional factors (low BC and toxic tall fescue) resulted in overall lower productivity in cows.     

Effect of iodine,selenium and cobalt rumen boluses given to dry dairy cows on the immunoglobulin and thyroid hormone status of calves

The objective was to determine the effect of rumen boluses containing 6800 mg iodine, 1000 mg selenium and 1000 mg cobalt given to dry dairy cows on the efficiency of colostral immunoglobulin G (IgG) absorption in calves. Thirteen cows received the bolus approximately 58 days before calving. A further 12 cows received no bolus and were used as controls. The cows were housed as one group. Calves were prevented from suckling for the first 24 h of life, and were given three feeds of a fixed quantity of colostrum. At 24 h, the average plasma concentrations of IgG in the calves were 15.5 and 13.4 g/L for the control and bolus groups, respectively; these were not significantly different (P = 0.212). Bolus treatment was associated with higher levels of free and total tri‐iodothyronine (T3) and thyroxine (T4) in the dams (all P < 0.001), although it had no effect on thyroid hormone levels in the calves. There were nevertheless positive and negative relationships between the efficiency with which colostral IgG was absorbed at 24 h and, respectively, total T3 at 24 h (P < 0.05) and total T4 at 1 h of age (P < 0.05). The underlying basis for these relationships remains to be established.     

Differences in copper and selenium metabolism between Angus (Bos taurus) and Brahman (Bos indicus) cattle

A 2-yr study was conducted at the Range Cattle Research and Education Center, University of Florida – Institute of Food and Agricultural Sciences (IFAS) (Ona, FL), to evaluate differences in the metabolism of Cu and Se of Angus (Bos taurus) and Brahman (Bos indicus) cattle. Thirty-two pregnant beef cows (n = 8 Brahman and 8 Angus/yr) were enrolled in the study in the first trimester of gestation. This study consisted of three phases: 1) restriction (day 0 to 90), 2) supplementation (day 91 to 150), and 3) calving. During all three phases, cows were individually fed and housed in partially covered drylot pens. During the restriction and supplementation phases, cows were provided a 1.5 kg/d of a grain-based concentrate supplement, which was fortified with flowers of S (50 g of supplemental S/cow daily; restriction phase) or Cu and Se (100 and 3 mg/d of Cu and Se, respectively; supplementation phase). Blood and liver samples were collected from all cows at 30 d intervals and from both cows and calves within 24 h of calving. Colostrum and milk samples were collected at calving and 7 d after birth. All data were analyzed using the MIXED procedure of SAS, where cow and calf were the experimental unit. During the restriction phase, a breed × day effect (P = 0.03) was observed where Brahman had greater liver Cu concentration than Angus cows in all sampling days. For liver Se concentration, a tendency (P = 0.07) for a breed effect was observed where Angus cows tended to have greater liver Se concentration than Brahman. During the supplementation phase, breed (P < 0.001) and day (P < 0.01) effects were observed, where Brahman cows had greater liver Cu concentration than Angus. For liver Se concentration, a day effect (P < 0.001) was observed, where liver Se concentration increased (P < 0.001) from day 90 to 120 and remained unchanged (P = 0.86) until day 150. At calving, no effects of breed (P = 0.34) were observed for liver Cu concentration of cows; however, Brahman calves tended (P = 0.09) to have greater liver Cu concentration than Angus calves. For Se liver concentration at calving, Angus cows tended (P = 0.07) to have greater liver Se concentration than Brahman cows; however, no breed differences (P = 0.70) were observed for liver Se concentration of calves at birth. In summary, substantial differences in multiple indicators of Cu and Se status were observed between Angus and Brahman cattle, implying that Angus and Brahman cattle possibly have different mechanisms to maintain adequate Cu and Se status.     

Comparison of crossbred cows containing various proportions of Brahman in spring or fall calving systems. I. Productivity as two-year-olds

Productivity of 2-yr-old crossbred cows containing various proportions (0, 1/4 or 1/2) of Brahman breeding was evaluated using 203 spring-calving and 171 fall-calving heifers over a 3-yr period. All heifers were mated to Limousin sires. Percentage of cows exposed to breeding that weaned a calf was the only trait for which a significant crossbred cow group x season of calving interaction was found. Preweaning ADG and age-adjusted weaning weight increased as proportion Brahman breeding increased. Age-adjusted weaning weight was similar for the two groups because spring-born calves were weaned at an average age of 205 d and fall-born calves were weaned at an average age of 240 d. For weaning condition scores, an interaction between dam breed and proportion Brahman was detected; scores tended to be greater for calves out of 1/2 Hereford dams than for those out of 1/2 Angus dams, and this difference increased as proportion Brahman increased. Weaning conformation scores were similar for all calves. Age-adjusted weaning hip height increased as proportion Brahman breeding increased. Based on numbers of weaned calves, spring calving was more advantageous than fall calving. Averaged across both calving seasons, weaning weight tended to increase as proportion Brahman increased.     

Effects of breed type and age at first calving on length of productive life in a cow–calf system in Southeastern Mexico

The effect of age at first calving (AFC) and breed type (BG) on cow length of productive life in the herd (LPL) and cumulative kilograms of calves weaned per cow (KWC) was studied. Data on 313 Brahman, 183 Nellore, and 269 crossbred cows (undefined crosses of European × Zebu cattle) born from 1986 to 2002 were used. Three hundred and ninety-one out of the 765 cows had non-censored data. LPL curves by BG and AFC (<2.5, 2.5 to <3, 3 to <3.5, and ≥3.5 years of age) were obtained using life table procedures. The magnitude of the effect of AFC and BG on LPL was determined using Cox proportional hazard regression procedures. KWC data (n = 359) were analyzed by a linear model that included the effects of period of birth of the cow (in years), BG, and AFC. There was no difference in the survival curves between BG (p > 0.05), but there was for the hazard ratio of culling (p < 0.05). Brahman and Nellore cows produced more kilograms of calves (p < 0.05) than crossbred cows. AFC influenced LPL (p = 0.014) and KWC (p = 0.012). Cows with AFC <3 years of age stayed longer in the herd and weaned more KWC than those with a higher AFC (p < 0.05).     

Effect of tropically adapted sire breeds on preweaning growth of F1 Angus calves and reproductive performance of their Angus dams

The objective of this study was to determine the effect of tropically adapted sire breeds on preweaning growth performance of F1 calves and on reproductive performance of their Angus dams. Angus (A) cows were bred in two consecutive years (1992 and 1993) by AI using semen from Brahman (B; Bos indicus; n = 10), Senepol (S; Bos taurus; n = 10), and Tuli (T; Sanga; n = 9) bulls. A total of 82 B x A, 85 S x A, and 91 T x A calves were born. The statistical model included the fixed effects of year, sire breed, calf sex, sire breed x calf sex, and cow parity and the random effect of sire within sire breed. Birth weight, weaning weight, 205-d adjusted weaning weight, ADG from birth to weaning, and hip height at weaning were greater (P < .001) for B x A calves than for S x A or T x A calves. Greater differences were detected between sexes for B x A than for S x A and T x A (for all traits sire breed x calf sex, P < .05). Sire breed affected (P < .01) the percentage of unassisted calvings (B x A, 87%; S x A, 98%; and T x A, 100%) and tended (P < .10) to affect the percentage of calves that survived until weaning (B x A, 90%; S x A, 94%; and T x A, 98%). Sire breed of calf did not affect (P > .10) length of gestation, and sire breed did not affect the interval from calving to first observed estrus or pregnancy in Angus dams. These results demonstrate that preweaning growth performance of B x A calves was greater than that of either S x A or T x A calves. However, use of Brahman sires on Angus dams led to calving problems and tended to reduce the percentage of calves that survived until weaning. Thus, heavier weaning weights of B x A calves would be an advantage for cow-calf producers marketing calves, but heavier birth weights and calving difficulty attributed to Brahman sires would be a disadvantage.     

Polymorphisms in growth hormone gene and their associations with calf weight in Japanese Black cattle

The objectives of this study were to detect effective genetic polymorphisms of bovine growth hormone (bGH) gene associated with calf weight in Japanese Black cattle. Fifty‐eight sires and 47 breeding cows were used to detect the polymorphisms in exons by single‐strand conformation polymorphism (SSCP). Four homozygous and six heterozygous SSCP genotypes were identified in exon 5. Although each single nucleotide polymorphism (SNP) had been reported, these genotypes were caused by three SNPs at the nucleotide positions 2141, 2277 and 2291. Four haplotypes C‐C‐A, G‐C‐A, C‐C‐C and G‐T‐A were newly identified. It was suggested that other haplotypes not detected in this study may not exist, considering the allele frequencies reported in Bos taurus and Bos indicus, and the migrating process of native Japanese cattle. Thereafter, we examined associations between the detected polymorphic sites in exon 5 by PCR – restriction fragment length polymorphism and calf weight using 53 breeding dams and 135 calves. The birth weights of calves with haplotype G‐C‐A are significantly lighter and calves' weights produced by cows with such haplotype are also lighter at 30 days old, using regression analysis. Although further research is necessary, these results may serve as a useful criterion to select breeding stocks, especially in maternal abilities.     

Epidemiological investigation of Leptospira spp. in a dairy farming enterprise after the occurrence of three human leptospirosis cases

An epidemiological investigation was conducted in an unvaccinated dairy farming enterprise in which three workers on one of the milking herds (Herd 1) were diagnosed with leptospirosis due to serovars Hardjo (H) (n = 2) and Pomona (P) (n = 1) between January and March 2015. Blood and urine samples were collected from milking cows in Herd 1 (N = 230) and Herd 2 (N = 400), rising one‐ (R1, N = 125) and rising two‐year‐old (R2, N = 130) replacement heifers, and four pigs associated with Herd 1, in March 2015. Sera were tested using the MAT for serovars H, P, Copenhageni (C), Ballum (B) and Tarassovi (T), and urine samples were tested by qPCR. Seventy‐five per cent of 109 cows in Herd 1 and 36% of 121 in Herd 2 were seropositive (≥48), predominantly to H and P, and 23% of 74 cows in Herd 1 and 1% of 90 cows in Herd 2 were qPCR positive. Fifty‐five per cent of 42 R2 heifers were seropositive to T. No R1 and 17% of 42 R2 heifers were qPCR positive. Subsequently, all cattle were vaccinated for H and P, and Herds 1 and 2 were given amoxicillin. After the booster vaccination, 7% of 91 in Herd 1, 2% of 82 in Herd 2 and 11% of 38 R1 heifers (sampled as R2) were PCR positive. After the amoxicillin treatment, no cows in Herd 1 and 5% of 62 cows in Herd 2 were urine PCR positive. Calves and pigs were seropositive to H, P, C and B. Vaccination and antibiotic treatment appeared effective in reducing the risk of exposure of workers to vaccine serovars. However, evidence of non‐vaccine serovars indicated that workers likely remain at risk of exposure to Leptospira.     

Effect of Pre- and Post-Partum Feed Supplementation on the Productive and Reproductive Performance of Grazing Primiparous Brahman Cows

This experiment studied the combined effect of pre- and post-calving feed supplementation on the productive and reproductive performance of primiparous Brahman cows. The animals were 4 years old (420 kg live weight) and pregnant, and grazed rotationally on Stargrass (Cynodon nlemfuensis) pastures. Supplementation was given before and after (T1, n = 15), only before (T2, n = 15) or only after (T3, n = 16) calving, and there was an unsupplemented control (T4, n = 16). The supplement (1.5 kg/animal per day, 13.4 MJ digestible energy/kg dry matter and 20% crude protein) was given for 45 days before and/or after calving. Changes in body weight and body condition score and the productive behaviour of the calves were similar (p>0.05) among treatments. The concentration of urea in the plasma was low in the control group (T4) before and after calving. The animals in all the groups had a poor reproductive performance. The percentage of cows in oestrus was higher (p<0.05) in T1 (73.3%) than in T3 (37.5%). The interval from calving to conception (148.7±53.7 days) and the pregnancy rate (48.5%) were similar (p>0.05) among the treatments. The level of supplementation offered before and/or after calving did not improve the pregnancy rate.     

Could a trace mineral deficiency be associated with congenital chondrodystrophy of unknown origin (CCUO) in beef cattle in Australia?

Congenital chondrodystrophy of unknown origin (CCUO) has been reported in beef cattle worldwide. A trace mineral deficiency in pregnant dams has been suggested as causing the deformities seen in CCUO calves. An extended outbreak of CCUO in Australia between 2002 and 2009 provided an opportunity to examine the pattern of trace mineral status in gestating cows and the effect of parenteral trace mineral supplementation on a herd at risk of CCUO calves. A property with a strong history of CCUO was identified from a previous case–control study. A randomly selected sample of multiparous (8 to 10 years old) Angus and Angus cross cows (n = 20) was assigned to control (no injection) or supplement group given three injections at the recommended dose rate of chelated mineral supplement (Mn, Zn, Cu and Se) at 6‐week intervals in mid‐ to late gestation. Liver biopsies were performed to measure liver Mn, Zn, Cu and Fe at each injection time and 1 month post‐calving. Pasture samples were also collected for analysis. Supplemented cows had higher liver Cu levels compared to control cows (p < 0.001), but there was no difference between supplement and control cows for liver Zn, Fe or Mn. Liver Cu (p < 0.001), Fe (p < 0.001) and Zn (p < 0.001) fell during gestation and liver Mn increased after calving (p < 0.001). Three cows from the control group and two from the supplement group had liver zinc levels below the normal values at the end of the trial. Two cows from the control group and one from the treatment group gave birth to cows with signs of CCUO including superior brachygnathia. All cows except one from the control group had rough dry coats with excessive dander. The role of trace mineral status in the birth of CCUO calves, especially the role of zinc deficiency, requires further investigation.     

Calving and weaning characteristics of Angus-, Gray Brahman-, Gir-, Indu-Brazil-, Nellore-, and Red Brahman-sired F1 calves

Calving and weaning data from crossbred calves sired by five Bos indicus breeds and one Bos taurus breed were evaluated. Data included calving and weaning records of F1 calves out of multiparous Hereford cows and sired by Angus, Gray Brahman, Gir, Indu-Brazil, Nellore, and Red Brahman bulls. At calving, Angus-sired calves had shorter gestations and lower (more desirable) calving ease scores and were smaller than Bos indicus-sired calves. Among the Bos indicus crosses, Gir calves had the shortest gestations, lowest calving ease scores, lightest birth weights (P less than .05), and smallest cannon bone lengths and heart girths. Nellore calves had the longest gestations (P less than .05) and largest heart girths. Calves by Indu-Brazil sires had the highest calving ease scores, highest birth weights (P less than .05), and greatest cannon bone lengths (P less than .05). Gray Brahman- and Red Brahman-sired calves were similar and intermediate for all calving characters. At weaning, Angus-sired calves had gained slightly faster than the Gir crosses and weighed more but were shorter at the hip than Gir crosses. Gir calves gained the least preweaning, weighed the least, and were shortest at weaning of the Bos indicus crosses. The Nellore and Indu-Brazil crosses were intermediate in preweaning gain and weaning weight to the Gir and the Red and Gray Brahman but were tallest at weaning. Gray Brahman and Red Brahman calves gained the most and were heaviest at weaning but were not as tall as the Nellore and Indu-Brazil.     

Effect of indoor energy restriction level and management on beef production in Belgian Blue double‐muscled cow‐calf pairs

One hundred and twenty‐six cows were involved to investigate the impact of energy (E) restriction (100%, 90%, 80% and 70% of total energy requirements) during the indoor period and two calf management systems (CMS: suckling or rearing). An E restriction during the indoor period resulted in a loss of body weight, even for E100, which was compensated during the re‐alimentation period, but body weight remained lower in suckling than in non‐suckling cows. The cows yielded 213 full‐term gestations and 8 abortions. Abortion was not affected by E (p = 0.187) or CMS (p = 0.804). Calving interval was neither affected by E (p = 0.775) but tended to be longer in suckled cows (p = 0.087). E reduced body weight and body condition score (BCS) at the end of the calving interval, while CMS only affected BCS. E70 resulted in a higher colostrum yield than E100 and E 90 but immunoglobulin content was not affected (p = 0.759). Cow culling was not affected by E or CMS. It increased from 12.9% to 22.2% and 32.5% for cows with a BCS at parturition lower than 1.5 or between 1.5 and 2.0, or higher than 2 respectively. Calf birth weight was neither affected by E or CMS, but there was an interaction between E and dam age (p = 0.050). Increasing the E restriction tended to reduce calf survival (p = 0.089). Performance of the surviving calves was not affected by E, but suckling calves gained faster than rearing calves (p < 0.001). Beef production by the cow‐calf pair was not different between energy levels (p = 0.738), but it was 10.8% lower for E70 than for E100 cows. It is not appropriate to apply an E restriction of more than 10–20% for maximum cow reproductive traits and calf performance.     

Gestational, Periparturient and Preweaning Growth Traits of Holstein versus Gir × Holstein F1Crossbred Dairy Calves Born to Holstein dams

The objectives of this study were to assess the gestational, periparturient and preweaning growth traits of Holstein vs Gir × Holstein (G × H F₁) crossbred dairy calves. Holstein cows (n=64) pregnant with Holstein (H × H) calves (bulls, n=16; heifers, n=18) or G × H (bulls, n=8; heifers, n=22) calves were sampled from 60 days of gestation through calving for serum progesterone (P₄) and in utero placentome sizes, and at parturition for calving ease scores (CES), calf vigour scores (CVS), and placental characteristics. Post-calving calf measures included birth weight (day 0) and body weight (kg), hip width (HW), body length (BL), wither height (WH), hip height (HH) and heart girth (HG) through 42 days of age. Serum IgG and calf vital function tests (respiration rate (RR), heart rate (HR), rectal temperature (RT) and fecal scores) were recorded on days 1, 3, 7 and 14 of age (a.m. and p.m.). Serum gestational P₄ was higher (p<0.05) for cows with H × H than G × H calves. Placentome measurements in utero were greater (p<0.01) for H × H calves than for G × H calves, while at parturition placental characteristics did not differ (p0.10). Gestation length, CES and CVS also did not differ (p0.10) between H × H and G × H calves. Calf RR and HR were higher (p<0.05) for H × H than G × H calves at both a.m. and p.m., while RT, fecal scores and serum IgG did not differ (p0.10) between H × H and G × H calves. Birth weight did not differ (p0.10) between H × H and G × H calves within sex. Moreover, bulls did not differ (p0.10) in any of the growth measures between H × H and G × H calves, whereas H × H heifers at days 28 and 42 of age were greater (p<0.05) in all growth traits than G × H heifers. Nevertheless, the relative change in growth measures over time (days 0 to 42) did not differ (p0.10) between H × H and G × H calves. While subtle differences in gestational, early growth and vital function characteristics were observed, these data suggest very similar developmental profiles between H × H and G × H calves.     

Metabolic and luteal function in winter‐calving Spanish beef cows as affected by calf management and breed

This experiment aimed at evaluating the effect of calf management and breed on the metabolic and luteal function of post‐partum beef cows fed at maintenance. Fifty multiparous cows, 22 Parda de Montaña (PA) and 28 Pirenaica (PI), were assigned to either suckling once‐daily for 30 min (RESTR) or ad libitum (ADLIB) from the day after calving. Blood samples were collected to analyse metabolites [non‐esterified fatty acids (NEFA), β‐hydroxybutyrate, total protein and urea)], insulin‐like growth factor‐I (IGF‐I) and progesterone (P4) at different intervals. Cows from RESTR maintained their live‐weight (LW) over the first 3 months post‐partum, whereas ADLIB cows lost nearly 4% LW. Both genotypes showed similar LW gains during this period (p > 0.10). Calf daily gains were lower in RESTR than in ADLIB treatment (p < 0.05), but similar across breeds (p > 0.10). Milk and lactose production were lower in RESTR cows than in ADLIB (p < 0.05). Milk and protein yield were greater in PA than in PI breed (p < 0.05). Serum NEFA, total protein and urea were higher in PI cows suckling ADLIB than in the rest (p < 0.05). Cows from PI breed had greater NEFA values than PA ones on the first week post‐partum (p < 0.001). Circulating IGF‐I was not affected by suckling frequency, breed nor their interaction (p > 0.10). Suckling frequency, but not breed, affected the interval from calving to first ovulation (p < 0.001), being shorter in RESTR than in ADLIB cows. In conclusion, the ad libitum suckling practice improved cow milk yield and offspring gain compared to once‐daily suckling for 30 min from the day after calving, at the expense of impairing the onset of cyclicity. The effect of calf management was confounded with breed on the studied blood biochemical constituents, but any of these metabolites influenced the role of endocrine IGF‐I in these genotypes.     

Comparison of crossbred cows containing various proportions of Brahman in spring or fall calving systems: III. Productivity as three-, four-, and five-year olds

Productivity of 3-, 4-, and 5-yr-old crossbred cows containing various proportions (0, 1/4, or 1/2) of Brahman breeding out of Angus or Hereford dams was evaluated using 489 spring-calving and 427 fall-calving records collected over a 4-yr period. Cows were bred to Limousin sires for the first 3 yr and to Limousin and Salers sires the 4th yr. Interactions between crossbred cow group and season of calving were not significant. Percentage of cows exposed to breeding that weaned a calf increased (P less than .01) as proportion Brahman breeding increased, was higher (P less than .01) for cows out of Angus dams than for cows out of Hereford dams, and was higher (P less than .01) for spring-calving cows. As the proportion Brahman increased, the percentage requiring assistance at birth decreased, and cows out of Angus dams required less (P less than .05) assistance than those out of Hereford dams. Preweaning ADG, adjusted weaning weight, weaning conformation, weaning condition, and adjusted weaning hip height increased a proportion Brahman breeding increased. Spring-born calves gained .12 kg/d faster (P less than .01) than fall-born calves. However, weight at weaning was similar for the two groups; spring-born calves were weaned at an average age of 205 d, and fall-born calves were weaned at an average age of 240 d. Fall-calving cows were heavier (P less than .05) than spring-calving cows, and 0 and 1/2 Brahman cows were heavier (P less than .01) than 1/4 Brahman cows. These data indicate that Brahman-cross dams can be used to improve reproductive rate and increase preweaning growth rate, and thus weaning weight.(ABSTRACT TRUNCATED AT 250 WORDS)     

Effect of supplementation of vitamin E,copper and zinc on the in vitro phagocytic activity and lymphocyte proliferation index of peripartum Sahiwal (Bos indicus) cows

To study the effect of vitamin E (VE), copper (Cu) and zinc (Zn) supplementation on the in vitro phagocytic activity (PA) and lymphocyte proliferation response (LPR) of blood neutrophils and lymphocytes, thirty Sahiwal pregnant cows (six in each group) in their late gestation at 30 days before the expected date of calving were selected from the NDRI experimental herd and supplemented with various micronutrients from 30 days before calving to 45 days after calving. Cows were supplemented individually with VE (1000 IU/cow/day), Cu (20 ppm/cow/day) and Zn (80 ppm/cow/day) and also with a combination of VE, Cu and Zn to study cumulative effect of all micronutrients. One group without any supplementation acted as a control. Blood neutrophils and lymphocytes were isolated and studied for their PA and LPR. Supplementation of micronutrients like VE, Cu, Zn and a combination of all these nutrients significantly (p < 0.01) increased the PA of experimental cows as compared to control (unsupplemented) cows during the pre‐partum period. During post‐partum, all the micronutrients (VE, Cu, Zn and their combination) showed a significant (p < 0.01) increase in the PA of experimental cows as compared to control cows. Of all the groups, significant (p < 0.01) and maximum PA was observed in the combination group followed by Zn‐supplemented group during both the pre‐ and post‐partum period. A significant (p < 0.01) increase in LPR of B lymphocytes was observed in combination‐supplemented group during the pre‐partum period and during both the pre‐ and post‐partum period in the Cu‐supplemented group.     

Comparison of crossbred cows containing various proportions of Brahman in spring- or fall-calving systems: IV. Effects of genotype x environment interaction on lifetime productivity of young cows.

Lifetime productivity of young (2 to 6-yr-old) crossbred cows containing various proportions (0, 1/4, or 1/2) of Braham breeding was evaluated using 188 spring-calving and 154 fall-calving cows. Cows were mated to Limousin sires to produce 1983 through 1986 calf crops. The 1987 calf crops were produced using Limousin and Salers sires. Significant (P less than .10) genotype (crossbred cow group) x environment (season of calving) interactions were found for age at first calf and lifetime calving percentage and mature cow weight. Within the spring calving group, no differences were found between crossbred groups for age at first calf or lifetime calving percentage. However, within the fall-calving group, 0 and 1/2 Braham cows from Angus dams calved earlier than did those from Hereford dams. Age at first calf also tended to increase as proportion of Brahman increased in the fall-calving group. Within the fall-calving group, lifetime calving percentage for 0 and 1/2 Brahman cows from Hereford dams was lower than for those from Angus dams. The opposite was true for the 1/4 Braham crosses. In general, as the proportion of Brahman breeding increased, productivity, measured as weight per calf or weight weaned per year, increased; however, age at first calving increased also as proportion of Brahman increased. All spring-calving groups calved earlier in life (P less than .05) than their respective fall-calving counterparts and with the exception of the 0 Brahman groups, which were similar, spring-calving groups produced a higher (P less than .05) percentage of calves than did the fall groups.(ABSTRACT TRUNCATED AT 250 WORDS)     

Hepatic steroid inactivating enzymes,hepatic portal blood flow and corpus luteum blood perfusion in cattle

Production from the corpus luteum (CL) and/or hepatic steroid inactivation impacts peripheral concentrations of P4, which can alter reproductive performance. Our primary objective was to examine hepatic steroid inactivating enzymes, portal blood flow, and luteal blood perfusion at 10 days post‐insemination in pregnant versus non‐pregnant beef and dairy cows. Twenty early lactation Holstein cows and 20 lactating commercial beef cows were utilized for this study. At day 10 post‐insemination, hepatic portal blood flow and CL blood perfusion were measured via Doppler ultrasonography. Liver biopsies were collected and frozen for later determination of cytochrome P450 1A (CYP1A), 2C (CYP2C), 3A (CYP3A), uridine diphosphate‐glucuronosyltransferase (UGT) and aldo‐keto reductase 1C (AKR1C) activities. Pregnancy was determined at day 30 post‐insemination and treatment groups were retrospectively assigned as pregnant or non‐pregnant. Data were analyzed using the mixed procedure of SAS. Steroid metabolizing enzyme activity was not different (p > .10) between pregnant versus non‐pregnant beef or dairy cows. Hepatic portal blood flow tended (p < .10) to be increased in pregnant versus non‐pregnant dairy cows. Luteal blood perfusion was increased (p < .05) in pregnant versus non‐pregnant dairy cows. Pregnant dairy cows appear to have an increased rate of hepatic clearance of P4 in combination with increased synthesis from the CL. This could account for the lack of difference in peripheral P4 concentrations between pregnant and non‐pregnant dairy cows. This study highlights the relevance of further investigation into steroid secretion and inactivation and their impact on the maintenance of pregnancy in cattle.     

Gastro-intestinal parasitism of cattle in native pasture grazing system in Guadeloupe (French West Indies).

Gastro-intestinal parasitism of creole cows and calves, Limousin x creole cross calves and Brahman x creole cross calves was recorded during seasons of calving in a native grazing system in Guadeloupe, a wet tropical area. Calvings were pooled during the dry season for two herds and during the rainy season for two other herds. For each calving season, calves of one herd were drenched monthly with anthelmintic. A marked periparturient rise in egg excretion was observed in cows. Coccidial infection was always present in calves, but no clinical signs were observed. The main parasites during the first 2 months of life were Strongyloides papillosus and Toxocara vitulorum. Then, Haemonchus placei, Trichostrongylus spp. and Cooperia spp. were the dominant species encountered. Brahman cross calves were the most heavily infested animals. There was no clear relationship between third stage larvae (L3) population size on pasture and worm burdens in calves, except during the second month of life. Despite medium levels of infestation, parasitism inhibited the growth of creole calves: -10.5 kg of bodyweight at weaning (-59 g day-1 of daily bodyweight gain from birth to weaning). The pathological effects of subclinical parasitism were confirmed by a lower packed cell volume and albuminaemia in parasitized calves than in treated calves.     

Prevalence of abortion and stillbirth in a beef cattle system in Southeastern Mexico

Prenatal mortality is an important cause of production losses in the livestock industry. This study estimates the prevalences of abortion and stillbirth in a beef cattle system and determines the significance of some risk factors, in the tropics of Mexico. Data were obtained from a Zebu cattle herd and their crosses with Bos taurus breeds, in Yucatan, Mexico. The logit of the probability of an abortion or stillbirth was modeled using binary logistic regression. The risk factors tested were: year of abortion (or calving), season of abortion (or calving), parity number and dam breed group. The effect of twins on stillbirth was tested using Fisher exact test. Of the 4175 calvings studied 49 were abortions (1.17%). Significant factors in the logistic regression analysis for abortions were season of abortion and parity number. The risk of abortion was lower in the dry seasons compared to the rainy and windy seasons (P = 0.009). The risk of abortion was higher in second parity cows followed by the third and first parity cows, as compared to older cows (P = 0.015). Of the 4126 births, 87 were stillbirths (2.11%). Significant factors in the logistic regression analysis for stillbirth were year of calving (P = 0.0001) and parity number (P < 0.001). The risk of stillbirth in first parity cows was 2.6 times that of old cows. Of the total births, 15 were twins (0.36%) of which 7 were born dead calves. Herd owners must focus on the significant risk factors under their control to reduce the prevalence of prenatal mortality.