贵州种子植物新记录(Ⅵ)

川滇木莲Manglietia duclouxii Finet et Gagnep.in Bull.Soc.Bot.France Mem 4:33.1905,et in Contr.Fl.I,Asie Orient.f.Ⅰ:33.1907;中国树木志1:424.1983.-Magnolia duclouxii (Finet et Gagnep.)Hu in Hu et Chun,Icon.pl.Sin.2:18.pl.68.1929. 常绿乔木,叶薄革质,无毛,倒披针形或倒卵状披针形,长8～13 cm,宽2.5～4 cm,无毛.托叶痕长约为叶柄长的三分之一.花梗长3.5 cm以下,直立,花被9,外3瓣红色,背面具疣状突起,雌蕊群有长毛.聚合果卵状椭圆形,长5～6 cm,有毛.     

Fiber Quality Improvement by Interspecific Hybridization in Upland Cotton

In order to transfer genes for extra fiberstrength to existing cotton cultivars,weintroduced 207 interspecific materials obtainedby outcrossing where G. hirsutum,G.barbadense,G.sturtianum,G.thurberi,G.harknessii and G.somalense etc.were involved asparents respectively.Under stringent evaluation     

山西省野生苔草属植物资源调查及坪用性状比较

通过调查发现山西省野生苔草属（Carex）植物资源有27种。对引种的14个种类野生苔草属植物坪用观赏性状观测,结果表明其中白颖苔草（C. stenophylloides V. Krecz.）,无脉苔草（C. enervis C.A.Mey.）,尖嘴苔草（C. leiorhyncha C.A.Mey.）,异穗苔草(C. heterostachya Bge.),寸草苔（C. duriuscula C. A.Mey.）和早春苔草（C. subpediformis Suto et Suzuki.）适合做草坪植物。     

贵州省淫羊藿属(小檗科)特有和珍稀植物及其保护价值

报道贵州省小檗科淫羊藿属特有植物(或已知仅贵州分布植物)10种1变种,分别为黔北淫羊藿(E.borealiguizhouenseS.Z.He & Y.K.Yang)、短茎淫羊藿(E.brachyrrhizum Steam)、德务淫羊藿(E dewuense S.Z.He,Probst & W.F.xu)、普定淫羊藿(E.pudingense S.Z.He,Y.Y.Wang & B.L.Guo)、多花淫羊藿(E.multiflorum T.S.Ying)、剑河淫羊藿(E.myrianthum Steam var.jianheense S.Z.He & B.L.Guo)、小叶淫羊藿(E.parvifolium S.Z.He & T.L.Zhang)、拟巫山淫羊藿(E.pseudowushanense B.L.Guo)、贵州淫羊藿[E.sagittatum (Siebold & Zuccarini)Maximowicz var.guizhouense S.Z.He & B.L.Guo]、水城淫羊藿(E.shuichengense S.Z.He)和单叶淫羊藿(E.simplicifolium T.S.Ying);易危植物2种,分别为小叶淫羊藿和单叶淫羊藿,被《中国物种红色名录》收录.描述了贵州淫羊藿属特有植物和珍稀植物的药用和保护价值及其分布产地.对淫羊藿属存在的问题进行总结分析,并提供贵州省小檗科淫羊藿属植物新名录和以贵州标本命名的淫羊藿属新物种名录.     

Detection of Chlorogenic Acid,Macranthoidin B and Dipsacoside B in FLOS LONICERAE Grown in Guizhou Province by UPLC-PDA-ELSD

[Objective] To detect chlorogenic acid,macranthoidin B and dipsacoside B in FLOS LONICERAE grown in Guizhou Province by UPLC-PDA-ELSD,and to investigate the quality of FLOS LONICERAE grown in Guizhou Province. [Methods] ACQUITYUPLC BEH C18 chromatographic column( 2. 1 mm × 100 mm,1. 7 μm) was adopted; mobile phase was acetonitrile( A)- 0. 4% acetic acid water( B); gradient elution was as follows: 0- 2 min,7% A; 2- 2. 3 min,7%- 28% A; 2. 3- 10 min,28% A; 10- 10. 5 min,28%- 7% A; gradient flow rate was as follows: 0- 2 min,0. 35 m L / min; 2- 2. 3 min,0. 35- 0. 25 m L / min; 2. 3- 2. 6 min,0. 25- 0. 15 m L / min; 2. 6- 2. 8min,0. 15- 0. 1 m L / min; 2. 8- 4 min,0. 1- 0. 2 m L / min; 4- 4. 3 min,0. 2- 0. 3 m L / min; 4. 3- 4. 5 min,0. 3- 0. 35 m L / min; 4. 5-10. 5 min,0. 35 m L / min; PDA( ultraviolet detector) was used to detect the chlorogenic acid( 330 nm wavelength); ELSD( evaporative light- scattering detector) was used to detected the macranthoidin B and dipsacoside B; temperature of the drift tube was 60 ℃; sprayer was48 ℃; gas pressure was 137. 9 k Pa; and column temperature was 50 ℃. [Results] Chlorogenic acid,macranthoidin B and dipsacoside B showed good linear relationship within the ranges of 0. 304 5- 1. 522 5 μg / m L( R2= 0. 999 4),0. 449- 1. 436 8 μg / m L( R2= 0. 999 3)and 0. 044 6- 0. 223 μg / m L( R2= 0. 999 4),respectively. [Conclusions] This method could accurately detect the contents of chlorogenic acid,macranthoidin B and dipsacoside B in FLOS LONICERAE grown in Guizhou Province,effectively shorten the measurement time and save reagents,so that it was suitable to detect the quality of FLOS LONICERAE grown in Guizhou Province.     

柚木cry1A(b)基因的遗传转化

1INTRODUCTIONTeakhasworldwidereputationasthemostdurabletimber .Teakisasun loving ,deciduoustree ,whichthrives,inanywell drainedsoil.Ithasagestation periodofbetween 15and 2 5 yearsde pendingontheregionwhereitisgrown .Owingtodwindlingnaturalteakforests ,teakhasbeenafavouritechoiceforagroforestry .However ,duetoitslonggestationperiod ,teakisnotaspopularasrubberoroilpalm .Neverthelessteakplantingisbe comingpopularinPeninsularMalaysiaandSabah .Witharapidgrowthratetogetherwithitssuperiortimbe…     

徐本美论著目录(1979～2002)

一、论文1 .徐本美 ,顾增辉 ,郑光华 .测定种子活力方法的探讨 (1 ) TTC法 .种子 ,1 982 ,(2 ) :1 22 .徐本美 ,顾增辉 .种子活力及其测定法 .北京农业科学 ,1 983,(1 ) :353.徐本美 ,顾增辉 .测定种子活力方法的探讨 ( )玻板直立发芽法 .种子 ,1 983 (4) :2 24 .徐本美 ,顾增辉 .棉花萌动种子的活力及能量变化初探 .中国棉花 ,1 983,(6) :95.徐本美 ,顾增辉 .用虫萤光素酶测定油料种子的 ATP含量 .中国油料 ,1 983,(1 ) :736.徐本美 ,顾增辉 ,任祝三 .测定种子活力方法之探讨 ( )电导法 .种子 ,1 983,(1 ) :1 87.徐本美 ,顾增辉 .大粒和…     

Determination of Selenium in Liangshan Yi Medicine by GFAAS

[Objective] To discuss the trace selenium contents in five Yi medicines,including Potentilla discolor Bunge,Lysimachia congestiflora Hemsl, Rohdea japonica( Thunb.) Roth, Rumex hastatus D. Don and Rhododendron rubiginosum Franch. var. rubiginosum.[Methods]Sample was digested by microwave digestion method. Ni( NO3)2- Mg( NO3)2solution was used as mixed matrix modifier. Selenium contents in the five Yi medicines were detected by graphite furnace atomic absorption spectrometry( GFAAS). [Results] Selenium contents in five samples were between 0. 095 9 and 1. 411 μg / g. The standard curve equation was A = 0. 005 6C + 0. 001 73,R = 0. 998 9. The linear range was 0. 20- 2. 0 ng / mL. The recovery rate was 95. 1%- 101. 3%,and RSD was 3. 73%- 7. 56%( n = 8). [Conclusions] This method was simple,rapid,accurate and reliable,and could be used for the content detection of selenium in Yi medicine.     

花生野生近缘种生物素羧基载体蛋白基因的克隆与结构分析

根据栽培花生鲁花14生物素羧基载体蛋白基因accB1和accB2 cDNA序列设计基因特异引物,克隆了花生野生近缘种Arachis duranensis、A.rigonii、A.batizocoi和A.hoehnei的accB1和accB2 cDNA 序列.序列分析表明,accB1和accB2在栽培花生和野生近缘种中高度保守,氨基酸序列一致性分别高于98.6%和97.5%. A基因组A.duranensis、A.rigonii和B 基因组A.batizocoi、A.hoehnei 的accB1和accB2的氨基酸序列存在差异,但与基因组来源无关.花生野生近缘种A.duranensis、A.rigonii、A.batizocoi和A.hoehnei accB2基因组序列分析表明,栽培花生和野生近缘种的accB2的基因结构相同. A基因组的A.duranensis与B 基因组的A.batizocoi和A.hoehnei的accB2 基因组DNA核苷酸序列一致性分别为98.9%,98.8%,而A 基因组的A.rigonii与A.duranensis、A.batizocoi和A.hoehnei 的序列一致性分别为93.5%,93.6%,93.4%.研究表明,生物素羧基载体蛋白基因在栽培花生及野生近缘种中非常保守.     

国外花卉组织机构一览

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