INFLUENCE OF HIGH TEMPERATURE AND UREA FERTILIZATION WITH N-(N-BUTYL) THIOPHOSPHORIC TRIAMIDE AND DICYANDIAMINDE ON COTTON GROWTH AND PHYSIOLOGY

The objective of this growth chamber study was to evaluate the effect of adding N-(n-butyl) thiophosphoric triamide (NBPT) and dicyandiaminde (DCD) to urea fertilizer, on the physiology and growth of cotton (Gossypiumhirsutum L.) under normal and high temperatures. Treatments consisted of two day temperature regimes, 30°C and 38°C, and five nitrogen fertilization applications: unfertilized control, 125 kg ha^?1 of urea, 93 kg ha^?1 of urea, 93 kg ha^?1 urea + NBPT, and 93 kg ha^?1 urea + NBPT + DCD. The addition of NBPT to urea fertilizer had positive effects on leaf chlorophyll, leaf area, dry matter, nitrogen (N) uptake, and N use efficiency. The absence of a significant interaction effect indicated that N fertilization was not influenced by temperature. Deficiency of N significantly decreased leaf chlorophyll, increased glutathione reductase, decreased protein and increased leaf nitrate reductase. Physiological changes under high temperature included increased plant N uptake, glutamine synthetase, leaf chlorophyll, protein content, plant height and leaf area were due to high N uptake and utilization.     

Die Rolle der mikrobiellen Biomasse und des mineralisch fixierten Ammoniums bei den Stickstoff-Transformationen in niedersächsischen Löß-Ackerböden unter Winter-Weizen. II. Umsetzung von 15N-markiertem Stickstoff

Significance of microbial biomass and mineral fixed ammonium with respect to the nitrogen transformations in loess soils of Niedersachsen during the growing season of winter wheat. II. ¹⁵N-turnover Field experiments 1988/89 on a fallow plot of the southern Niedersachsen loess area with straw application (δ 10 t · ha^?1, homogeneously incorporated by hand) yielded an increase in microbial biomass-N (N_mic) by 60 kg N · ha^?1 · 30 cm^?1 until March 1989 and further 40 kg N · ha^?1, till May which was almost completely remobilized until harvest. For a cropped plot (with winter wheat and 10 t · ha^?1 straw incorporation), N immobilization was of similar magnitude. Up to 18% of the applied ¹⁵N-fertilizer (185 kgN · ha^?1) were microbially immobilized. In contrast to 1988/89, no significant mass change of N_mic occurred in 1991 due to straw application (δ 10t · ha^?1). Variations in the amount of N_mic were nearly independent on the treatment (crop, with 140 kg fertilizer-N · ha^?1 or without N-fertilizer, respectively; fallow plot without fertilizer-N) within a range of 225-400 kg N · ha^?1 · 30 cm^?1. Directly after N-application (each 70 kg N · ha^?1 in March and in May), up to 100% of the fertilizer-N were assimilated by the microbes. Subsequently, remobilization of the immobilized nitrogen occurred within 2 (in March) or 6 weeks (in May), respectively. Simultaneously, organic soil-N was mineralized after each N-application and minerally fixed for us biggest part. Between March and June, the fixed NH₄+ decreased by about 112 kg · ha^?1 · 30 cm^?1.     

Simultane Bestimmung von N-Transformationsraten in Bodensäulen unter Verwendung von 15-N: Stickstoffmodell für eine Terra fusca-Rendzina

Simultaneous determination of nitrogen transformation rates in soil columns using 15-N: N-Model of a Terra fusca-Rendzina soil Rates of ammonification, nitrification, immobilization, and denitrification were determined in undisturbed columns of a Terra fusca Rendzina soil. A steady input of 15-N labelled ammoniumsulfate with the irrigation water created a steady state of the turnover processes in the soil resulting in a constant output of 15-N-nitrate. In this state the rate constants (8°C) were K₁ = 0.64 for the netto-N-nitrification, K₂ = 0.11 for the netto-N-denitrification, and K₃ = 0.25 for the netto-N-immobilization. 64% of the nitrate was leached, 25% immobilized in organic matter, and 11% denitrified. Relating these rate constants to the turnover of the soil nitrogen one can calculate the mean annual rates for the different processes of a forest soil, using the mean annual temperature. For the Göttinger Wald situation (T = 6.9°C) the following rates were calculated; Ammonification = 183 kg N·ha^?1·a^?1, immobilization = 44 kg N·ha^?1·a^?1, netto N-denitrification = 19 kg N·ha^?1·a^?1, and netto-N-mineralization = 120 kg N·ha^?1·a^?1.     

Die Rolle der mikrobiellen Biomasse und des mineralisch fixierten Ammoniums bei den Stickstoff-Transformationen in niedersächsischen Löß-Ackerböden unter Winter-Weizen. I. Poolgrößenveränderungen

Significance of microbial biomass and non-exchangeable ammonium with respect to the nitrogen transformations in loess soils of Niedersachsen during the growing season of winter wheat. I. Change of pool sizes Nitrogen transformations in loess soils have been examined by laboratory and field experiments. After straw application (· 8 t · ha^?1), N in microbial biomass (N_mic) increased by about 20 mg · kg^?1 soil (· 90 kg N · ha^?1 · 30 cm^?1) after 9 days of incubation (20 °C). Another laboratory experiment yielded an increase of about 400 mg of NH₄+-N · kg^?1 fixed by minerals within 1 h after addition of 1 M NH₄+-acetate. Defixation of the recently fixed NH₄+ after addition of 1 M KCl amounted to only 60 mg · kg^?1 within 50 days. In a field experiment with winter wheat 1991, an increase in N_mic of about 80 kg N · ha^?1 · 30 cm^?1 was observed from March to June. After July, growth of the microbes was limited by decreased soluble carbon concentrations in the rhizosphere. Different levels of mineral N-fertilizer (0, 177 and 213 kg N · ha^?1) did not affect significantly the microbial biomass. The same field experiment yielded a decrease of non-exchangeable ammonium on the “zero”-fertilized plot in spring by 200 kg N · ha^?1 · 30 cm^?1. The pool of fixed ammonium increased significantly after harvest. After conventional mineral N-fertilizer application (213 kg N · ha^?1). NH₄+-defixation was only about 120 kg N · ha^?1 · 30 cm^?1 until July.     

Zeolite influences on nitrate leaching,nitrogen-use efficiency,yield and yield components of canola in sandy soil

With regard to the low cation-exchange capacity and large saturated hydraulic conductivity of sandy soils, a field experiment was carried out in 2006–2007 to determine the impact of zeolite on nitrogen leaching and canola production. Four nitrogen (N) rates (0, 90, 180, and 270 kg ha^–1) and three zeolite amounts (3, 6 and 9 t ha^?1) were included as treatments. The results demonstrated that the highest growth parameters and seed yield were attained with 270 kg N ha^?1 and 9 t zeolite ha^?1. However, the highest and the lowest seed protein percentage and oil content were obtained with 270 kg N ha^?1 accompanied by 9 t zeolite ha^?1, respectively. Nitrate concentration in drained water was affected by nitrogen and zeolite. The lowest and highest leached nitrate values were found in control without N and zeolite (N₀Z₀) and in treatments with the highest N supply without zeolite (N₂₇₀Z₀), respectively. In general, nitrogen-use efficiency decreased with an increase in N supply. Application of 9 t zeolite ha^?1 showed higher nitrogen use efficiency than other zeolite amounts. Also, application of more N fertilizer in soil reduced nitrogen uptake efficiency. In total, application of 270 kg N ha^?1 and 9 t zeolite ha^?1 could be suggested as superior treatment.     

Effects of controlled-release urea combined with conventional urea on nitrogen uptake,root yield,and quality of Platycodon grandiflorum

Field experiments were conducted with four nitrogen fertilizer treatments to study the effects of controlled-release urea combined with conventional urea on the nitrogen uptake, root yield, and contents of protein, soluble sugar, saponin, zinc (Zn), iron (Fe), magnesium (Mg), and copper (Cu) in Platycodon grandiflorum. Field experiments were conducted with four nitrogen (N) fertilizer treatments: no N fertilization; conventional urea with N rate of 175 kg N ha^?1; conventional urea with N rate of 160 kg N ha^?1; controlled-release urea combined with conventional urea with N rate of 160 kg N ha^?1; controlled-release urea combined with conventional urea with N rate of 135 kg N ha^?1. The results showed that nitrogen application significantly increased the yield of P. grandiflorum compared with the control. Treatment with controlled-release urea combined with conventional urea at 160 kg N ha^?1 provided the highest yield of 7329.58 kg ha^?1. Nitrogen application also increased the contents of soluble sugar, total saponin, protein, Zn, Fe, and Mg but decreased Cu content. Protein, saponin, and Zn contents were significantly higher, but Cu content was lower in P. grandiflorum fertilized with controlled-release urea combined with conventional urea than those fertilized with conventional urea alone. The combination of controlled-release urea with conventional urea at 160 kg N ha^?1 was the optimal treatment under the experimental condition investigated in this study.     

Denitrifikation eines gemüsebaulich genutzten Bodens in Abhängigkeit von der Einarbeitung frischer Erntereste

Denitrification losses from a horticultural soil as affected by incorporation of fresh plant residues Denitrification in the A_p-horizon of a Luvisol under horticulture as affected by fresh cauliflower residues (Brassica oleracea L. var. botrytis) was measured using the acetylene inhibition technique. The residues were chopped and ploughed in 25 cm deep. Denitrification rates, soil temperature, moisture, nitrate and watersoluble carbon were determined from the end of July until the end of September. One day after incorporation of plant residues the denitrification rate showed a rapid increase to the highest value (5.15 kg N · ha^?1 · day^?1) of the experimental period. During the following 57 days the rates declined to the level of the untreated control. The highest denitrification rates occurred in the experimental sites which received plant residues. The estimated N-loss for the whole experimental period (57 days) totalled 43.6 kg N · ha^?1 in the treatment with plant residues and 2 kg N · ha^?1 in the treatment without plant residues. Coefficients of variation of denitrification rates were high (29–206%). Within the treatment ‘with plant residues’ denitrification rate and watersoluble carbon were positively correlated.     

Influence of Organic Residue Recycling on Crop Yield,Nutrient Uptake,and Microbial and Nutrient Status of rabi Sorghum (Sorghum bicolor L.) under Dryland Condition

The field experiment was conducted on black soil (Vertic Ustropept) at Zonal Agricultural Research Station farm, Solapur, for successive 30 years from 1987–1988 to 2016–2017 under dryland condition in a randomized block design with 10 treatments and 3 replications. The pooled results of seven years (2010–2011 to 2016–2017) revealed that the application of 25 kg N ha^?1 through crop residue (CR, byre waste) along with 25 kg N ha^-1 through Leucaena lopping (Leucaena leucocephala) to rabi sorghum gave significantly higher grain and stover yield and Sustainable Yield Index (14.61 and 36.11 q ha^?1 and 0.47, respectively) which was on par with T₇, where 25 kg N ha^?1 through farmyard manure (FYM) + 25 kg N ha^?1 through urea was applied for grain and stover yield (13.95 and 34.46 q ha^?1 and 0.44, respectively). The gross and net monetary returns and benefit–cost ratio were also influenced significantly due to integrated nitrogen management (Rs. 59,796, Rs. 47,353 ha^?1, and 3.13, respectively). This was also reflected in residual soil fertility status of soil after harvest of rabi sorghum. The organic carbon content and available nitrogen content of soil, as well as nitrogen uptake and moisture use efficiency for grain, were also increased. The total microbial count of bacteria, fungi, and actinomycetes was more where FYM or CR addition was done. The count of N fixers and P solubilizers was more under Leucaena application either alone or with CR or urea. Application of CR at 4.8 t ha^?1 (25 kg N ha^?1) along with Leucaena lopping at 3.5 t ha^?1 (25 kg N ha^?1) as green leaf manure is the best alternative organic source for fertilizer urea (50 kg N ha^?1) to increase the production of dryland rabi sorghum.     

Nitrogen application in direct wet-seeded rice under alternate wetting and drying irrigation condition: Effects on grain yield,dry matter production,nitrogen uptake and nitrogen use efficiencies

Yield, dry matter production, nitrogen (N) uptake and nitrogen use efficiency (NUE) of Bangladesh Rice Research Institute (BRRI) dhan29 were investigated during two consecutive dry (Boro) seasons of 2009–10 and 2010–11. The experiments were set up in a randomized complete block design with three replication having six nitrogen (N) levels of 0, 40, 80 120, 160 and 200 kg ha^?1. Nitrogen fertilization increased yield characters, dry matter production and N uptake. The economic optimum rate of N was 166 and 155 kg ha^–1 in first and second year, respectively, with corresponding yield of 7.1 and 6.5 t ha^?1. NUEs were higher in the first year, decreased with increasing N rates in most cases. Gross return over fertilizer reached the highest Tk 692 in 2009–10 and Tk 489 in 2010–11 with 160 kg N ha^–1. The results suggest that BRRI dhan29 should receive an average of 160 kg N ha^?1 for economic optimum yield.     

Effect of nitrogen application on growth and yield of pumpkin

Evaluation of any crop response to different nitrogen amounts is important for determining the amount that can be considered as optimum from economical and environmental point of view. This study was conducted to (1) evaluate the growth and yield of pumpkin (Cucurbita pepo L.) under different nitrogen rates and (2) determine the nitrogen use efficiency (NUE) of pumpkin in two growing seasons (2013 and 2014). In both growing seasons, nitrogen fertilizer (at three rates including 50, 150, and 250 kg ha^?1) was band-dressed on the planted side of each furrow, coinciding with 4–6 leaves stage and flowering. Crop performance over 2 years was evaluated by measuring shoot dry matter, crop growth rate (CGR), leaf area index (LAI), leaf area duration (LAD), intercepted PAR (PAR_i), radiation use efficiency (RUE), shoot nitrogen uptake, water use efficiency (WUE), NUE, and fruit and seed yield. The results showed that in both growing seasons, the highest growth and yield of pumpkin were obtained by applying 250 kg N ha^?1 (using urea fertilizer containing 46% nitrogen). Increased nitrogen rate from 50 to 250 kg ha^?1 resulted in 87.3%, 27.0%, 62.1%, 87.5%, and 84.5% increase in shoot dry weight, RUE, WUE, fruit yield, and seed yield of pumpkin, respectively, across both growing seasons. However, higher application nitrogen rate decreased the NUE of pumpkin, i.e., the NUE decreased by 62.5% when the nitrogen rate increased from 50 to 250 kg ha^?1. The effect of nitrogen applied in 2014 growing season on growth and yield of pumpkin was higher than that in 2013 growing season, which might be due to more suitable weather condition. In conclusion, the nitrogen rate of 250 kg ha^?1 produced the highest amount of fruit and seed yield in pumpkin.     

Effect of soybean inoculation with Bradyrhizobium and wheat inoculation with Azotobacter on their productivity and N turnover in a Vertisol

A long-term field experiment was conducted for 8 years on a Vertisol in central India to assess quantitatively the direct and residual N effects of soybean inoculation with Bradyrhizobium and wheat inoculation with Azotobacter in a soybean–wheat rotation. After cultivation of soybean each year, its aerial residues were removed before growing wheat in the same plots using four N levels (120, 90, 60 and 30 kg ha^?1) and Azotobacter inoculation. Inoculation of soybean increased grain yield by 10.1% (180 kg ha^?1), but the increase in wheat yields with inoculation was only marginal (5.6%; 278 kg ha^?1). There was always a positive balance of soil N after soybean harvest; an average of +28 kg N ha^?1 yr^?1 in control (nodulated by native rhizobia) plots compared with +41 kg N ha^?1 yr^?1 in Rhizobium-inoculated plots. Residual and direct effects of Rhizobium and Azotobacter inoculants caused a fertilizer N credit of 30 kg ha^?1 in wheat. Application of fertilizers or microbial inoculation favoured the proliferation of rhizobia in crop rhizosphere due to better plant growth. Additional N uptake by inoculation was 14.9 kg N ha^?1 by soybean and 20.9 kg N ha^?1 by wheat crop, and a gain of +38.0 kg N ha^?1 yr^?1 to the 0–15 cm soil layer was measured after harvest of wheat. So, total N contribution to crops and soil due to the inoculants was 73.8 kg N ha^?1 yr^?1 after one soybean–wheat rotation. There was a total N benefit of 13.8 kg N ha^?1 yr^?1 to the soil due to regular long-term use of microbial inoculants in soybean–wheat rotation.     

Residual nitrogen effects on wheat following legumes in the southern plains

Increasing nitrogen (N) fertilizer prices give rise to the question of N benefits from legumes in cropping systems in the Southern Great Plains. This study quantified wheat (Triticum aestivium L.) hay production and N uptake over seven years following six years of alfalfa (Medicago sativa L.), cicer milkvetch (Astragalus cicer L.), or grass (Old World bluestem, Bothriochloa ischaemum L.) production in western Oklahoma. Precipitation over the seven years averaged 550 mm·yr^‐1. The major residual N effects were measured within the first five years. On a fine sandy loam soil, wheat hay yields averaged 3,070 kg·ha^‐1·yr^‐1 over five years following alfalfa, 2,580 kg·ha^‐1·yr^‐1 following milkvetch, and 950 kg·ha^‐1·yr^‐1 following grass with N uptake attributed to the residual effect from legumes (calculated by the difference method) averaged 34 kg N ha^‐1·yr^‐1 from alfalfa and 25 kg·ha^‐1·yr^‐1 from milkvetch. On a deep loamy sand soil, wheat hay yields averaged 1,290 kg·ha^‐1·yr^‐1 over five years following alfalfa and 710 kg·ha^‐1·yr^‐1 following grass with N uptake attributed to the residual effect from alfalfa averaged 8 kg N ha^‐1·yr^‐1. Thus, the residual N effect attributed to legumes was substantial on the fine sandy loam soil and relatively small on the deep loamy sand soil.     

Eucalyptus biochar application enhances Ca uptake of upland rice,soil available P,exchangeable K,yield, and N use efficiency of sugarcane in a crop rotation system

It was hypothesized that the application of eucalyptus biochar enhances nutrient use efficiencies of simultaneously supplied fertilizer, as well as provides additional nutrients (i.e., Ca, P, and K), to support crop performance and residual effects on subsequent crops in a degraded sandy soil. To test this hypothesis, we conducted an on‐farm field experiment in the Khon Kaen province of Northeastern Thailand to assess the effects of different application rates of eucalyptus biochar in combination with mineral fertilizers to upland rice and a succeeding crop of sugarcane on a sandy soil. The field experiment consisted of three treatments: (1) no biochar; (2) 3.1 Mg ha^?1 biochar (10.4 kg N ha^?1, 3.1 kg P ha^?1, 11.0 kg K ha^?1, and 17.7 kg Ca ha^?1); (3) 6.2 Mg ha^?1 biochar (20.8 kg N ha^?1, 6.2 kg P ha^?1, 22.0 kg K ha^?1, and 35.4 kg Ca ha^?1). All treatments received the same recommended fertilizer rate (32 kg N ha^?1, 14 kg P ha^?1, and 16 kg K ha^?1 for upland rice; 119 kg N ha^?1, 21 kg P ha^?1, and 39 kg K ha^?1 for sugarcane). At crop harvests, yield and nutrient contents and nitrogen (N) use efficiency were determined, and soil chemical properties and pH₀ monitored. The eucalyptus biochar material increased soil Ca availability (117 ± 28 and 116 ± 7 mg kg^?1 with 3.1 and 6.2 Mg ha^?1 biochar application, respectively) compared to 71 ± 13 mg kg^?1 without biochar application, thus promoting Ca uptake and total plant biomass in upland rice. Moreover, the higher rate of eucalyptus biochar improved CEC, organic matter, available P, and exchangeable K at succeeding sugarcane harvest. Additionally, 6.2 Mg ha^?1 biochar significantly increased sugarcane yield (41%) and N uptake (70%), thus enhancing N use efficiency (118%) by higher P (96%) and K (128%) uptake, although the sugar content was not increased. Hence, the application rate of 6.2 Mg ha^?1 eucalyptus biochar could become a potential practice to enhance not only the nutrient status of crops and soils, but also crop productivity within an upland rice–sugarcane rotation system established on tropical low fertility sandy soils.     

Fate of fertilizer nitrogen.

Results are presented from a three year lysimeter investigation, employing single (¹⁵NH₄NO₃) and double (¹⁵NH₄¹⁵NO₃) labelled ammonium nitrate to study the uptake of soil and fertilizer nitrogen by cut ryegrass at 250, 500 and 900 kg N ha^?1 a^?1. Average annual recoveries of nitrogen were equivalent to 99,76 and 50% of the nitrogen added at 250, 500 and 900 kg N ha^?1, respectively. At 250 kg N ha^?1 the difference between the overall nitrogen recovery and the fertilizer recovery was almost entirely attributable to pool substitution resulting from mineralization/immobilization turnover (MIT). At 900 kg N ha^?1 both the low overall recovery of nitrogen and the low fertilizer recovery reflected the large excess of available nitrogen over crop requirements. No evidence of ‘priming’ was obtained. Analysis of the results from single and double labelled lysimeters using simultaneous equations indicated that at 250 kg N ha^?1,～70% of the nitrogen in the crop was derived from the ammonium pool. At 500 kg N ha^?1 this dropped to 64%, while at 900 kg N ha^?1 the figure was 59%. There was a suggestion that at the lower application rates, preferential uptake of ammonium was occurring but that as N supply exceeded crop requirements, nitrate was the major N source. Despite the preferential exploitation of the ammonium pool, at 250 and 500 kg N ha^?1 pool substitution resulting from MIT resulted in lower recoveries of fertilizer ammonium compared with fertilizer nitrate.     

Nitrogen Rate Influence on Pearl Millet Yield,Nitrogen Uptake,and Nitrogen Use Efficiency in Nebraska

Abstract

Pearl millet is a potential dryland crop for Nebraska. Experiments were conducted in eastern Nebraska in 2000, 2001, and 2002, and in western Nebraska in 2000 and 2001. The objectives were to determine optimum nitrogen (N) rate, N uptake, and N use efficiency (NUE) for pearl millet. The hybrids “68×086R” and “293A×086R” and N rates of 0, 45, 90, and 135 kg N ha^?1 were used. Hybrids had similar yield, N uptake and NUE responses. In western Nebraska in 2000, pearl millet yield response to N rate was linear, but the yield increase was only 354 kg ha^?1 to application of 135 kg N ha^?1. In eastern Nebraska, pearl millet response to N rate was quadratic with maximum grain yields of 4040 in 2001 and 4890 kg ha^?1 in 2002 attained with 90 kg N ha^?1. The optimum N rate for pearl millet was 90 kg N ha^?1 for eastern Nebraska. For western Nebraska, drought may often limit pearl millet's response to N fertilizer.     

Influence of organic mulching and nitrogen application on essential oil yield and nitrogen use efficiency of rosemary (Rosmarinus officinalis L.)

In a field study conducted at Bangalore, India for two years (2003–2004 and 2004–2005), eight treatment combinations consisting of two variables, organic mulch (lemongrass spent material as mulch at 7.5 t ha^?1 and no mulch) and four levels of nitrogen fertilizer (0, 100, 200 and 300 kg ha^?1) were examined to observe the effect of organic mulching on nitrogen use-efficiency, and herb and essential oil yield in a multiharvested rosemary crop. The results revealed that application of lemongrass spent material as mulch increased the herb and essential oil yields in rosemary by 16.2 and 24.2%, respectively, over the non-mulched control at first harvest. Corresponding values for the regenerated crop harvest were 18.8 and 16.8%. A significant response to N was observed with 300 kg N ha^?1 in non-mulched plots compared with 200 kg N ha^?1 in mulched plots. Using lemongrass spent material as mulch, nitrogen uptake by the rosemary crop increased by 15.1% over the non-mulched control. At 200 kg N ha^?1, apparent recoveries by the crop were estimated to be 33.64% for the non-mulched control, and 37.79% with mulch. The quality of the rosemary essential oil, in terms of the concentrations of its major constituents, α-pinene, 1 : 8 cineole, camphor and verbenone, was not affected by the use of organic mulching and nitrogen fertilization, and these constituents were found to be of astandard acceptable in international trade.     

Agronomic Assessment of Nitrogen Use Efficiency in Spring Wheat and Interrelations with Leaf Greenness Under Field Conditions

Four spring wheat genotypes (Triticum aestivum L.) were grown without (N0 = 0 kg N ha^?1) and under ample (N1 = 250 kg ha^?1) nitrogen (N) fertilizer in field experiments in two seasons. The aim was to assess genotypic variation in N use efficiency (NUE) components and N-related indices during grain filling thus to identify superior wheat genotypes. Leaf chlorophyll (SPAD) readings at crucial growth stages were employed to help differentiate genotypes. Interrelations between yield and N-related indices with SPAD, where also assessed to explain possible pathways of improving NUE early in the growing season. Results showed that genotypic effects on NUE were mostly evident in 2000, a year with drier preanthesis and wetter postanthesis than the normal periods. ‘Toronit’ almost always had the highest biomass yield (BY) and grain yield (GY). Except in 1999 under N0, ‘L94491? showed the highest % grain N concentration (GNC). Genotypes affected SPAD at almost all stages and N fertilization delayed leaf senescence for all genotypes and growth seasons. Correlations between SPAD at different growth stages and GY, N biomass yield at maturity (NBYM) and GNC were significant (P≤ 0.001), positive and strong/very strong (>r = 0.7). N translocation efficiency (NTE) was inversely related to PANU (~r = ? 0.77, P≤ 0.001), suggesting that N after anthesis is being preferentially transported to the ears to meet the N demand of the growing grains. It is concluded that there is still a large potential for increased NUE by improved N recirculation, use of fast and inexpensive crop N monitoring tools and high yielding, N uptake efficient genotypes.

Abbreviations: NUE, Nitrogen use efficiency; SPAD, Minolta SPAD-502 chlorophyll meter, NHI, nitrogen harvest index; HI, Harvest index; NTE, N translocation efficiency from vegetative plant parts to grain; DMTE, dry matter translocation efficiency; CPAY, contribution of pre-anthesis assimilates to yield; PANU, Post-anthesis N uptake, d.a.s., days after sowing, N0, zero (0) kg ha^?1 applied N fertilizer, N1, 250 kg ha^?1 applied N fertilizer.     

The effect of different nitrogen levels on yield and quality of stevia (Stevia rebaudiana bert.)

In the present work, the efficiency of different nitrogen doses (0, 50, 100, 150, and 200 kg ha^?1) on growth, yield, and quality of stevia (Stevia rebaudiana Bert.) was investigated in 2011–2013. The study was conducted in Antalya located in the Mediterranean Region of Turkey. Terra rossa type soil (LVx, FAO) characteristics of the experimental field were clay loam, with high amounts of lime (33,9%) and slightly alkaline (pH 7.7). The experiment was carried out in randomized block design with four replications. All the results were summarized as mean of three years. The highest fresh and dry biomass yields (26.75 t ha^?1 and 7.5 ha^?1, respectively) were obtained from 150 kg ha^?1 N dose and followed by 100 kg ha^?1 N dose (26.29 t ha^?1 and 7.24 ha^?1, respectively). Whereas the highest fresh and dry leaf yields (13.27 t ha^?1 and 3.82 t ha^?1, respectively) were realized in 100 kg ha^?1 N dose. Actually, all nitrogen doses gave higher biomass and leaf yields compared to the control. On the hand, major steviol glycosides (stevioside and rebaudioside A) in the leaf were not influenced by nitrogen levels. In conclusion, 100 kg ha^?1 N dose was found to be suitable for cultivation of stevia under field conditions.     

Evaluation of the 15Nitrogen Isotope Dilution Technique for Estimating Crop Nitrogen Uptake from Organic Residues

Abstract

A field experiment was conducted to test the new approach for estimating crop nitrogen (N) uptake from organic inputs. The soil was prelabeled with ¹⁵N by applying ¹⁵N fertilizer to sunflower crop (Helianthus annuus L. var. Viki). The ¹⁴N plots, which received unlabelled fertilizer, were also set up. At harvest, ¹⁵N labeled residues were added to the unlabeled soils at a rate of 73 kg N ha^?1 (direct technique) and unlabeled residues were added to the ¹⁵N‐labeled soils at the same rate (indirect technique). Control plots without residues were also established. All plots were sown with the wheat (Triticum aestivum L. var merchouch)–fababean (Vicia faba L.)–wheat (Triticum aestivum L. var merchouch) cropping sequence.

In the cropping sequence, the first, second and third crop derived respectively 12.01, 2.4, and 1.93 kg N ha^?1 from crop residues estimated by the direct method and 14.77, 3.3, and 1.85 kg N ha^?1 estimated by the indirect method. The results showed no significant difference between the two techniques, which suggests that the new soil prelabeling technique compares well with the direct technique.     

Interactive Effect of Phosphorus and Nitrogen on Leaf Anthocyanins,Tissue Nutrient Concentrations,and Dry‐Matter Yield of Floralta Limpograss during Short Day Length

Abstract

A field trial was conducted during the short‐day period of 2004–2005 at Ona, Fl., to study the factorial effect of nitrogen (67, 90, and 134 kg N ha^?1) and phosphorus (0, 5, 10, 20, and 40 kg P ha^?1) rates on forage dry‐matter yield, quality, nutrient uptake, and leaf pigment concentration of limpograss (Hemarthria altissima). The N and P fertilizers were applied 45 days before each of two harvests. There was no interaction between N and P rates on any of the measured variables. Cool‐season forage yield increased curvilinearly from 137 to 350 kg ha^?1 in winter and 237 to 1389 kg ha^?1 in early spring, whereas crude protein (CP) concentration increased from 145 to 158 g kg^?1, as P was increased from 0 to 40 kg ha^?1, but yield and CP were not affected by N rate. There was a decreasing linear relationship between leaf concentration of anthocyanins and P rate of application such that forage obtained with 0 kg P ha^?1 had 61% more leaf anthocyanins and purple pigmentation than with 40 kg P ha^?1. There was no effect of N on anthocyanins content. It was concluded that increased level of leaf anthocyanins was due to the cumulative stress from cool weather and lower plant‐tissue P levels, which resulted in reduced growth and yield of limpograss. In cool weather, P played a critical role in controlling leaf purple pigmentation and forage yield.