Assessment of zinc availability to rice in mollisols of North India

Abstract

The objectives of this study were to (1) characterize zinc (Zn) fractions and their relation to Zn extracted with mixed‐bed ion exchange resin capsules and (2) assess the relationships between the latter and Zn uptake by rice in 12 Mollisols from North India. The Resin Adsorption Quantity (RAQ) of Zn was measured after 1 and 14 days of anaerobic incubation. Six organic and inorganic Zn fractions were determined on anaerobic soil. Zinc uptake by rice was studied in a greenhouse experiment. Soil Zn fractions under reduced conditions followed the order residual Zn (80%)>carbonates and amorphous oxides bound Zn (12%)>weakly organically bound Zn (3%)>crystalline oxides bound Zn (2%)>strongly organically bound Zn (2%)>water soluble + exchangeable Zn (1%). RAQ‐Zn was best correlated with Zn bound to carbonates and amorphous oxides. Due to negative interactions between bicarbonate and Zn uptake, correlations between relative dry matter yield or total Zn uptake and the different Zn fractions, DTPA‐Zn measured on dry soil, or RAQ Zn were not significant. Adjusting soil test values according to soil pH improved the prediction of relative dry matter yield, but further studies are required to determine whether the resin capsule can be used as a soil test for Zn in calcareous soils.     

The effect of aging biosolids on the availability of cadmium and zinc in soil

A major concern with the safe re‐use of biosolids on land is the potential for release of metals from organic matter in the biosolids, due to decomposition proceeding as biosolids age. To quantify the effects of biosolid aging on Cd and Zn bioavailability, two sewage sludges (Lagoon sludge and Filtered sludge) and a garden compost were incubated at 25°C and 35°C for 100 days. Changes in availability of Cd and Zn were determined using isotope dilution principles, with the materials being labelled with carrier‐free ¹⁰⁹Cd and ⁶⁵Zn. We determined isotopically exchangeable metal pools (E values) and plant available metal pools (L values) by measuring specific activities of Cd and Zn in soil extracts and in wheat plants, respectively. Changes in carbon content over time were determined using ¹³C‐NMR spectroscopy and chemical extraction methods, and related to changes in availability of metal pools as determined by isotopic procedures. Hot‐water‐extractable carbon content, assumed to represent easily decomposable organic matter, decreased during the 100 days by 80–190 mg kg^?1. The Compost and Lagoon sludge showed no change in L values for Cd or Zn with time, but in the Filtered sludge the L values for Cd and Zn increased significantly, by 43% and 56%, respectively. The isotopically exchangeable pools of Cd and Zn did not change with incubation treatment of the biosolids. These data indicate that the potential for metal release from biosolids as organic matter decomposes depends to a large extent on the biosolid composition.     

Interaction between manganese and zinc in growth of rice plants

Many instances of antagonistic relationships among various micro-elements and between micro- and macro-elements in their absorption, translocation, and functions in plant growth have been reported (1). In a series of our researches on the effect of micro-elements on the growth of rice plants, it was observed that manganese toxicity symptom of rice plants was markedly reduced by increasing the concentration of zinc in the nutrient sdution. Although it has been reported that manganese toxicity symptoms of crops were reduced by addition of Fe (2,3), A1 (4), Ca (5) and silicate (6) to the medium, there have been few reports of the effect of Zn on Mn toxicity of rice plants. In this paper, the interaction between Mn and Zn in the growth of rice plants was reported.     

The effect of temperature regime and substrate Mn on growth and manganese concentrations in rice

Three rice cultivars, IR712, M1–48 and E425 were grown in solution culture for 10 weeks in temperature controlled glasshouses at IRRI, Los Baños, Philippines. Treatments consisted of two Mn levels, 0.5 and 50 μg.cm^‐3 and two temperature regimes, 35/27 and 20/20 (day/night) in °C. Dry matter and Mn concentrations were determined at 2‐week intervals. In a second experiment, the M1–48 cultivar was grown in solutions containing 0.5, 1, 40 and 80 μg.cm^‐3 Mn at 35/27, 29/21 and 20/20 °C (day/night) temperatures.

It was concluded that rice is more sensitive to excess Mn under cool conditions and that the concentration of Mn in the tissue at which toxicity symptoms appear or growth is decreased depends on the age of the tissues and the environmental conditions under which the plant is growing. Growth of two‐day old seedlings exposed to excess Mn was decreased to a greater degree than was that of 2‐week old seedlings. It is suggested that in screening rice cultivars for tolerance to excess Mn the environmental conditions should be rather rigidly controlled.     

The effect of two endogeic earthworm species on zinc distribution and availability in artificial soil columns

The objective of this study was to determine the impact of earthworm bioturbation on the distribution and availability of zinc in the soil profile.Experiments were carried out with Allolobophora chlorotica and Aporrectodea caliginosa in 24 perspex columns (∅ 10 cm), filled with 20-23 cm non-polluted soil (OM 2%, clay 2.9%, pH 0.01 M CaCl₂ 6.4), that was covered by a 3-5 cm layer of aged zinc spiked soil (500 mg Zn/kg dry soil) and another 2 cm non-polluted soil on top. After 80 and 175 days, columns were sacrificed and each cm from the top down to a depth of 15 cm was sampled. Earthworm casts, placed on top of the soil, were collected. Each sample was analyzed for total and CaCl₂-exchangeable zinc concentrations.Effects of earthworm bioturbation were most pronounced after 175 days. For A. chlorotica, total and CaCl₂-exchangeable zinc concentrations in the polluted layers were lower with than without earthworms. Total zinc concentrations in the non-polluted layers were higher in columns with earthworms. Casts of A. chlorotica collected on the soil surface showed slightly higher total zinc concentrations than non-polluted soil. Casts were found throughout the whole column. For A. caliginosa there were no differences in total zinc concentration between columns with and without earthworms. CaCl₂-exchangeable zinc concentrations in the polluted layers were lower for columns with earthworms. Casts were mainly placed on top of the soil and contained total zinc concentrations intermediate between those in non-polluted and polluted soil layers.This study shows that different endogeic earthworm species have different effects on zinc distribution and availability in soils. A. chlorotica transfers soil throughout the whole column, effectively mixing it, while A. caliginosa decreases metal availability and transfers polluted soil to the soil surface.     

Long-term effects of tillage on the availability of iron, copper, manganese, and zinc in a Spanish Vertisol

Organic matter accumulation and increased microbial activity under no-till can affect the dynamics of some essential micronutrients for plants. The main purpose of this work was to study the long-term effect of tillage on the availability of Fe, Mn, Cu, and Zn in a calcareous soil from Southern Spain. To this end, nutrient availability in surface soil (0–5-cm depth) subjected to a long-term tillage experiment (21 years) was evaluated via pot experiments and chemical tests involving DTPA extraction (as availability index) and sequential chemical fractionation of Mn and Fe.Soil organic matter (SOM) content and microbial activity (estimated by the β-glucosidase method) were found to be significantly higher under no-till (NT) than under conventional (CT) or minimum tillage (MT). Also, DTPA extractable Mn, Cu, and Zn, and citrate–bicarbonate extractable Mn (Mn_cb), were all higher under NT than under CT and MT, the differences being related to the increase in SOM as revealed by the correlation of Mn, Cu, and Zn extractable with DTPA and SOM (r = 0.87, P < 0.001; r = 0.8, P < 0.01, and r = 0.86, P < 0.001, respectively), and that between Mn_cb and SOM (r = 0.87, P < 0.001). However, the increased extractability resulted in no increased concentrations of these nutrients in plants. Moreover, the Mn concentration in the last expanded leaf was significantly lower with NT than with CT, which can be ascribed at least partly to an increased microbial activity under NT as revealed by the negative correlation between Mn in plants and β-glucosidase activity in soil (r = −0.71, P < 0.01). The Fe concentration in plants was not affected by soil tillage; also, it was only related to citrate–ascorbate extractable Fe (r = 0.69, P < 0.05), which exposes the contribution of poorly crystalline Fe oxides in soil to Fe nutrition in plants.     

The effect of Lumbricus rubellus and Lumbricus terrestris on zinc distribution and availability in artificial soil columns

This study investigated the impact of epigeic and (epi)anecic earthworms on the distribution and availability of zinc in the soil profile. Experiments were carried out with Lumbricus rubellus and Lumbricus terrestris in perspex columns (Ø 10 cm), filled with 20 to 23 cm non-polluted soil [organic matter 2%, clay 2.9%, pH 6.4 (0.01 M CaCl₂)], that was covered by a 3- to 5-cm layer of aged zinc-spiked soil (500 mg Zn/kg dry soil) and another 2 cm non-polluted soil on top. After 80 days, columns were sacrificed and sampled in a depth profile. Earthworm casts, deposited on top of the soil, were collected. Each sample was analyzed for total and 0.01 M CaCl₂-exchangeable zinc concentrations. L. rubellus did not go deeper than 3 cm into the soil and therefore no effect on zinc distribution in the soil could be detected. For L. terrestris, total zinc concentrations in the non-polluted layers were slightly but significantly higher in columns with earthworms, and so were the CaCl₂-exchangeable zinc concentrations in the polluted layers of these columns. Casts of L. terrestris collected from the soil surface showed higher total zinc concentrations than those from non-polluted soil. Casts were mainly placed on top of the soil. This study showed that these epigeic and (epi)anecic species have only a slight effect on zinc availability, and that deep burrowing species, like L. terrestris, are able to transport polluted soil from deeper layers to the soil surface.     

土壤锌、铁、铜、锰形态的分布及其与植物有效性的关系

The distribution of various fractions of Zn,Fe,Cu and Mn in 15 types of soils in China and its relationship with plant availability were studied.Fractions of various elements were found to have some similar characterstic distribution regularities in various types of soils,but various soil types differed to varying degrees in the distribution of each fraction.Soil physico-chemical properties,such as pH,CEC and the contents of OM,CaCO3,free Fe,free Mn and P2O5,were significantly correlated with the distribution of elemental fractions,and a significant correlation also existed between the distribution and plant availability of elemental fractions.Various fractions of each element were divided into two groups based on their plant availability.The correlation between the distribution of combination fractions and plat availability indicated a significantly or an extremely significantly positive correlation for Group I but a significantly or an extremely significantly negative correlation for Group II.Therefore,the fractions in Goup I were primary pools of available nutrients,while those in Group II could hardly provide available nutrients for plants.Decreasing the transformation of corresponding elements into fractions of Group II and increasing the storage capacity of various fractions of Group I were an important direction for regulation and controlling of soil nutrients.However,some Particular soils with too high contents of Zn,Fe,Cu and Mn should be regulated and controlled adversely.     

秸秆还田对砂姜黑土理化性质与锰、锌、铜有效性的影响

采用田间试验与实验室培育试验研究作物秸秆还田对砂姜黑土理化性质及Cu、Zn、Mn有效性的影响,结果表明,秸秆还田可降低土壤容重,增大土壤总孔隙度,特别是毛管孔隙度显著增加。土壤中胡敏酸和富里酸含量显著增加,胡敏酸含量的增加对提高土壤有机质活性和改善土壤肥力具有良好效应。与单施化肥比较,秸秆配施化肥土壤中交换态锰、锌、铜含量分别增加11%、21%和41%,有机结合态锰、铜分别增加19%和103%。培育试验表明,加入粉碎的作物秸秆培育90d后,土壤中有效锰、有效铜含量分别增加21%和27%。     

干旱条件下氮、磷水平对土壤锌、铜、锰、铁有效性的影响

研究了干旱条件下不同N、P水平对微量元素Zn、Cu、Mn、Fe有效性的影响。结果表明 ,在中壤质土壤含水量 11%～ 14%的情况下 ,不同N、P配比明显增加了土壤剖面中速效P的含量 ,但随着土壤深度的增加 ,土壤速效P呈不断下降的趋势。大田和土壤培养试验表明 ,施P有增加土壤Zn、Cu、Mn有效性的趋势 ,但有效Fe则有下降的趋势。土壤速效P、Zn、Cu、Mn、Fe之间的相关分析表明 ,土壤速效P与有效Zn之间存在显著的正相关关系 (r =0.65) ,而与有效Fe之间则存在显著的负相关关系 (r =- 0.63)。     

Regulation of zinc and manganese transport into developing wheat grains having different zinc and manganese concentrations

Grains of wheat were produced with differing zinc (Zn) or manganese (Mn) contents by culturing detached ears from anthesis onwards in solutions of four different concentrations of Zn or Mn (0.1,1.0,10, and 50 μM). After 20 days, ears were labeled with ⁶⁵Zn or ⁵⁴Mn at (i) the pretreatment concentrations of Zn or Mn, or (ii) at 10 μM Zn or Mn regardless of the pretreatment. Accumulation of Zn or Mn in the grain was greater as the pretreatment concentration of Zn or Mn increased from 1.0 to 10 μM, however, accumulation was less in ears cultured at 50 μM Zn or Mn. Accumulation of Mn in grain of different Mn status labeled at 10 μM Mn was similar in the 0.1,1.0, and 10 μM Mn pretreatments, but accumulation in the grain pretreated at 50 μM Mn was reduced. In contrast, accumulation of Zn in grains of different Zn status when labeled at 10 uM Zn was highest in ears pretreated at 10 μM Zn, but substantially lower in ears of a lower Zn status (those pretreated at 0.1 or 1.0 μM Zn) as well as in those pretreated at 50 μM Zn. These results suggest that Zn‐deficient grain was not a strong sink for Zn, while at high concentrations of solution Zn, a protective barrier exists preventing excessive accumulation of Zn in the grain. Proportionally more Zn was distributed to the inner pericarp and generally less to the endosperm, outer pericarp, and embryo as the Zn status of the grain increased. This work demonstrates that loading of Zn and Mn into, and distribution within, wheat grain is regulated by the nutritional status of the grain.     

淹水时间对水稻土磷素形态转化及其有效性的影响

水稻土淹水后土壤中P的形态及其有效性发生了变化,为掌握淹水时间对这种变化影响的规律,选择太湖地区典型水稻土-黄泥土为试验样本,在室内进行了不同淹水时间的土壤培育试验,并采用新鲜土壤样品测定的方法,研究了淹水时间对土壤P素形态转化及其有效性的影响.结果表明:①淹水时间主要影响Fe-P、O-P、Ca10-P和Ca8-P的转化.与淹水前相比,淹水使Fe-P和Ca8-P增加、O-P和Ca10-P减少,其中,Fe-P和Ca8-P分别于淹水45和15天后增加最为显著并趋于稳定,此时分别增加了194和9.6 mg/kg(分别占淹水前的93％和73％);O-P和Ca10-P于淹水15天后减少最为显著并趋于稳定,此时分别减少了72和20 mg/kg(分别占淹水前的24％和27％):②淹水改变了水稻土P素有效性及有效P的数量,与淹水前相比,淹水45天,水稻土 P素有效性及有效P的数量最高,有效P增加了32 mg/kg(占淹水前的57％).结论:淹水时间影响着水稻土 P素形态转化、有效性以及有效P的数量,淹水45天,水稻土P素形态转化数量最大、有效性最高和有效P的数量最大.     

The effect of various nitrogen fertilizers on zinc (zn) availability in non calcic brown forest soils

In this article the effect of different nitrogen fertilizer application on the availability of zinc (Zn) content in non calcic brown forest soil was investigated. For this purpose increasing rates of NH₄Cl, (NH₄)₂S0₄; NH₄N0₃ and Ca(N0₃)₂.4H₂0 fertilizers were applied for having different physical and chemical properties in two soil samples. An incubation experiment was carried out over 4 months. The available Zn content of the soils was determined each month. According to the results the highest available Zn content of the soils was obtained from NH₄Cl applied to the soils followed by (NH₄)₂S0₄; NH₄N0₃ and Ca(N0₃)₂.4H₂0 application to soil. At the end of the experiment the average available Zn content of non calcic brown forest soils for NH₄Cl, (NH₄)₂S0₄; NH₄N0₃ and Ca(N0₃)₂.4H₂0 fertilizers are 0.93, 0.91, 0.88 and 0.77 mg kg^?1, respectively.     

Genotypic differences in concentrations of iron,manganese, copper,and zinc in polished rice grains

Identification of genotypic differences in micronutrient concentrations of staple food crops is essential if plant breeding strategies are to improve human mineral nutrition. The concentrations of zinc (Zn), iron (Fe), copper (Cu), and manganese (Mn) in polished grains of 285 rice (Oryza sativa L.) genotypes and the relationship between concentrations of the four micronutrient elements and concentrations of protein and lysine were examined. Significant differences (P<.01) were found in the concentrations of Zn, Fe, Cu, and Mn in polished rice with a fairly normal distribution among rice genotypes. On average, Cu and Zn concentrations of Indica rice were about 2‐fold higher than Japonica rice, while Fe concentrations of Japonica rice were slightly higher than Indica rice. Among Indica rice genotypes, red rice contained higher Zn than white rice. Protein and lysine concentrations differed considerably among the genotypes, but no close relationship between the micronutrients and protein or lysine concentrations was observed among genotypes. Sixteen genotypes with significantly higher grain Zn, Fe, Cu, and Mn concentrations were identified.     

水稻僵苗不能盲目施锌肥

宝应县地处江苏里下河地区。主要粮食作物水稻的常年种植面积约 4万 hm2 。僵苗问题一直困扰着水稻生产。八十年代调查表明 ,全县土壤有效锌含量 0 .31～ 5.92 mg/kg;含量低于缺锌临界指标 1 .0 mg/kg的农田约 5.6 5万hm2 ,占耕地总面积 93.7%。其后很大程度上重视了稻田补锌。     

Effects of Sesbania rostrata and Azolla microphylla incorporation on transformation of applied zinc and copper in lateritic rice soils with different flooding regimes

Fixation of Zn and Cu applied to tropical rice-growing lateritic soils rich in Fe-oxides may be reduced if the soils are kept flooded for a few days before their application. There may be a further reduction if such flooding is combined with incorporation of green manures. To investigate this effect, a laboratory experiment was conducted to study the effect of different periods (0 and 15 days) of preflooding combined with (0 and 0.50% of soil weight) Sesbania rostrata and Azolla microphylla incorporation as green manures on the transformation of applied Zn and Cu in two lateritic rice-growing soils. Recovery of added Zn/Cu in DTPA (diethylene triamine pentaacetate)-extractable form was always found to be higher when they were applied after the soils were maintained in a flooded state for 15 days than when applied immediately after flooding; this effect was more prominent in respect of Cu. Contrary to expectations, green manure incorporation along with preflooding caused a significant decrease in recovery of Zn/Cu; the effect, however, showed a decreasing trend as incubation progressed. The effect was more marked with A. microphylla than with S. rostrata, particularly with Cu. Possible causes of such changes and their implications on the Zn/Cu nutrition of rice are discussed. Received: 7 August 1995     

不同阴离子形态锌肥对水稻营养及土壤锌形态的影响

盆栽试验研究3种阴离子形态Zn肥对水稻生长发育、养分吸收及土壤Zn形态的影响。结果表明,NO3-或NO3-与SO42-配合较SO42-、Cl-有利于提高水稻产量。供试土壤各形态Zn含量以交换态(Ex-Zn)和有机结合态(OM-Zn)最低,其次为碳酸盐结合态(CAB-Zn),再次为晶体氧化铁结合态(COFe-Zn)、无定型氧化铁结合态(AOFe-Zn)及氧化锰结合态(OMn-Zn),其中Ex-Zn、OM-Zn及OMn-Zn 3形态Zn之和(即土壤有效锌)为9.2mg/kg,占全Zn量的9.6%;土壤矿物态锌是土壤Zn素的主要组分,含量为69.3mg/kg,占全Zn量的72.0%。植稻提高了土壤CAB-Zn、AOFe-Zn、OMn-Zn含量,土壤各形态Zn仍以Ex-Zn、OM-Zn最低,其次为COFe-Zn,再次为CAB-Zn、AOFe-Zn及OMn-Zn含量,Ex-Zn、OM-Zn及OMn-Zn 3形态Zn之和为10.5mg/kg,占全Zn量的11.2%。土壤矿物态锌仍是土壤Zn素的主要组分,但含量及占全Zn量的比例较种植前分别减少4.2mg/kg和2.7%。水稻施Zn提高了植株吸Zn量和土壤全Zn量及OMn-Zn、COFe-Zn含量,土壤有效锌(Ex-Zn、OM-Zn及OMn-Zn 3形态之和)含量较对照增加12.7%~103.8%。