Role of the rhizosphere in utilization of inorganic iron-III-compounds by corn plants

Using a nutrient solution with nitrate-nitrogen, a strong interaction between iron and phosphorus uptake in water culture was observed. Iron chlorosis could be prevented only by a very high supply of iron-III-hydroxide or a very low supply of phosphorus, both of which resulted in a normal chlorophyll content but produced plants deficient in phosphorus. However when iron and phosphorus were supplied to separate root zones (split-root technique), iron-III-hydroxide was a satisfactory source of iron for corn plants even in water culture. In contrast to corn plants grown in water culture, plants in sand culture (quartz sand) with the same nutrient solution utilized iron-III-hydroxide just as well as iron chelate, even when high phosphorus concentrations were simultaneously present. Using ⁵⁹Fe and circulating the nutrient solution through the sand culture, it could be demonstrated that the mobilization of iron from iron-III-hydroxide is restricted to the root-sand (iron-III-hydroxide) interface (rhizosphere) without increasing the amount of soluble iron in the bulk substrate. The depletion of phosphorus around the roots in sand seems to be particularly responsible for this “substrate effect” in the utilization of iron-III-hydroxide. The uptake of phosphorus and iron in sequence along a root growing in a solid substrate could be important in the iron nutrition of “iron-inefficient” plant species such as corn growing in soils of high pH.     

Growth of Loblolly Pine and White Pine after Enrichment by Nutrient Loading

Low nutrient availability often constrains the growth of young trees following planting to fields or forests. Nutrient loading of young tree seedlings increases their growth in outplanting. Loblolly pine (Pinus taeda L.) and white pine (Pinus strobus L.) were grown for one year on nutrient-loading regimes that varied from 13 to 410 mg N L^?1 in sand culture. Other nutrients varied in proportion to the nitrogen (N) concentrations. Resulting plant growth showed that an array of nutritional regimes from deficient to excessive was applied. The young plants were transplanted into containers of sandy loam and were grown for one year without fertilization. Growth of each species increased with enhanced nutrient loading. The optimum concentration of N for nutrient loading was about 1.5 to 1.8% leaf dry weight for either species. Nutrient loading during nursery culture imparts transplants with nutrient levels that will enable growth for at least a year after outplanting.     

Nutrient availability and corn growth in a poultry litter biochar‐amended loam soil in a greenhouse experiment

Nutrient‐rich biochar produced from animal wastes, such as poultry litter, may increase plant growth and nutrient uptake although the role of direct and indirect mechanisms, such as stimulation of the activity of mycorrhizal fungi and plant infection, remains unclear. The effects of poultry litter biochar in combination with fertilizer on mycorrhizal infection, soil nutrient availability and corn (Zea mays L.) growth were investigated by growing corn in a loam soil in a greenhouse with biochar (0, 5 and 10 Mg/ha) and nitrogen (N) and phosphorus (P) fertilizer (0, half and full rates). Biochar did not affect microbial biomass C or N, mycorrhizal infection, or alkaline phosphomonoesterase activities, but acid phosphomonoesterase activities, water‐soluble P, Mehlich‐3 Mg, plant height, aboveground and root biomass, and root diameter were greater with 10 Mg/ha than with no biochar. Root length, volume, root tips and surface area were greatest in the fully fertilized soil receiving 10 Mg/ha biochar compared to all other treatments. The 10 Mg/ha biochar application may have improved plant access to soil nutrients by promoting plant growth and root structural features, rather than by enhancing mycorrhizal infection rates.     

Effect of water stress on aluminum toxicity in pitch pine seedlings

A decrease in soil water content during droughts may increase aluminum (Al) to concentrations that are toxic to the growth of trees. The effects of water stress (WS) on the response of ectomycorrhizal pitch pine (Pinus rigida Mill.) seedlings to aluminum was determined by growing seedlings in sand irrigated with nutrient solution (pH 3.8) containing 0, 5, or 10 mg L^‐1 Al. Water stress was imposed for 41 days by withholding nutrient solution for five consecutive days each week. At harvest time, seedlings at high WS had 72% of mean gravimetric water contents of seedlings at low WS. Aluminum decreased growth of seedlings at high WS, but had no effect on growth of seedlings at low WS. Aluminum toxicity symptoms in roots (e.g., dark thickened tips) were observed at lower Al levels at high WS than at low WS. Stem dry weight was the only plant part decreased by water stress alone. Across Al levels, Al concentration in roots was higher at low WS than at high WS. Water stress alone reduced root [phosphorus (P), potassium (K), and calcium (Ca)] and foliar [P, K, and magnesium (Mg)] concentrations of mineral nutrients. Decreases of nutrients in roots with increasing Al was greater at low than at high WS. Calcium was the only foliar nutrient decreased by Al treatment.     

Drought and salinity: A comparison of their effects on mineral nutrition of plants

The increasing frequency of dry periods in many regions of the world and the problems associated with salinity in irrigated areas frequently result in the consecutive occurrence of drought and salinity on cultivated land. Currently, 50% of all irrigation schemes are affected by salinity. Nutrient disturbances under both drought and salinity reduce plant growth by affecting the availability, transport, and partitioning of nutrients. However, drought and salinity can differentially affect the mineral nutrition of plants. Salinity may cause nutrient deficiencies or imbalances, due to the competition of Na⁺ and Cl^– with nutrients such as K⁺, Ca²⁺, and NO . Drought, on the other hand, can affect nutrient uptake and impair acropetal translocation of some nutrients. Despite contradictory reports on the effects of nutrient supply on plant growth under saline or drought conditions, it is generally accepted that an increased nutrient supply will not improve plant growth when the nutrient is already present in sufficient amounts in the soil and when the drought or salt stress is severe. A better understanding of the role of mineral nutrients in plant resistance to drought and salinity will contribute to an improved fertilizer management in arid and semi‐arid areas and in regions suffering from temporary drought. This paper reviews the current state of knowledge on plant nutrition under drought and salinity conditions. Specific topics include: (1) the effects of drought and salt stress on nutrient availability, uptake, transport, and accumulation in plants, (2) the interactions between nutrient supply and drought‐ or salt‐stress response, and (3) means to increase nutrient availability under drought and salinity by breeding and molecular approaches.     

Ursachen kleinräumiger Ertragsschwankungen im bayerischen Tertiärhügelland und Folgerungen für eine teilschlagbezogene Düngung

Site effects of small-scale yield variation in the Tertiary hills north of Munich (Germany) and conclusions for site specific farming The effect of numerous soil factors on small-scale yield variation of winter wheat and spring barley were examined: soil structure and soil texture, soil nitrate content and soil water at different times, P_CAL-, K_CAL-, N_t- and C_t-content, pH, soil microbiology characteristics, relief, root growth and important plant diseases. The varying annual influence of soil parameters on crop yield was interrelated with climatic factors. In soils with low sand content soil productivity was largely influenced by soil structure. This effect was less pronounced on soils with medium sand content. On sandy soils, however, yield was reduced by available water capacity. Yield potential was also lowered by frequent cereal growing associated with take-all root desease of winter wheat. High yield variation from year to year confirmed that a site-specific crop management should consider annual variability of yield in addition to soil conditions and yield measurement. Site-specific N fertilization should be adapted to the actual progress of plant growth.     

Einfluß von konstantem und abnehmendem Nährstoffangebot auf Aufnahme und Verteilung von K,Ca und Mg bei Sonnenblumen

Influence on the uptake of K, Ca and Mg in sunflowers with a constant and a diminishing supply of nutrients. . In a model-test with sunflowers it was investigated how the K-, Ca- and Mg-content in the plants is reacting when (a) a nutrient solution maintained constantly at the same level is given as in a ?good soil”? with a constant supply of nutrients, compared to (b) a solution with diminishing as in a highly fertilized soil but which hardly supplies nutrients itself. It was shown that, if the offer of the nutrient was constant, the cation content in the leaves could be maintained at the same level (the relation of cations is also maintained at the same time). Contrary to this with a diminishing nutrient solution due to removal of nutrients out of the solution without replenishing nutrients and the differentiated nutrient translocation the result was a lesser and lesser content of the nutrients inside the plant and more evident changes in the relation of cations, even before the insufficient supply was noted in a decreasing crop. Only in the leaves the relation of cations was nearly constant (K:Ca:Mg = 6:3:1). From these results it can be concluded that, through a high single supply of nutrients the effect of a constant flow of nutrients from the soil to the plant concerning the mineral contents of the plant cannot be compensated.     

Accumulation and distribution of Zn and Mn in soybean seeds after nutrient seed priming and its contribution to plant growth under Zn- and Mn-deficient conditions

Nutrient seed priming is a strategy to increase the seed reserves of mineral nutrients as primary source for mineral nutrition during seedling development and early growth. The present study investigates the effects of zinc (Zn) and manganese (Mn) seed priming on growth and nutritional status of soybean under conditions of Zn and Mn limitation. Nutrient seed priming increased the natural seed reserves for Zn by, approximately, sixfold and by fivefold for Mn; however, 40–60% of the primed nutrients were adsorbed to the seed coat. Zinc seed priming was able to maintain plant growth for 5 weeks in the same way as Zn supply via the nutrient solution. It is concluded that nutrient seed priming offers perspectives to improve seed quality of soybean for early seedling development under limited nutrient supply or availability and needs further investigation on performance under various stress conditions.     

Soil fertility in organic farming systems – fundamentally different?

Abstract. Soil fertility is defined as the ability of a soil to provide the conditions required for plant growth. It is a result of the physical, chemical and biological processes that act together to provide nutrients, water, aeration and stability to the plant, as well as freedom from any substances that may inhibit growth. Within this definition, it is useful to distinguish between those components of fertility which change relatively slowly, perhaps over the course of a rotation, or in some cases, decades, and the more immediate contribution from materials such as fertilizers and manures. The term 'inherent fertility' is used to describe these more stable characteristics, while recognising that they are, to a large extent, products of soil management. We conclude that, although nutrient management in organically managed soils is fundamentally different to soils managed conventionally, the underlying processes supporting soil fertility are not. The same nutrient cycling processes operate in organically farmed soils as those that are farmed conventionally although their relative importance and rates may differ. Nutrient pools in organically farmed soils are also essentially the same as in conventionally managed soils but, in the absence of regular fertilizer inputs, nutrient reserves in less-available pools will be of greater significance.     

NUTRIENT BIOAVAILABILITY IN SALT AFFECTED SOILS

Salt affected soils limit crop yields around the world. Knowledge of how nutrient availability is affected in plants growing on salt affected soils is important in adopting appropriate management practices to satisfy plants’ nutritional requirements and improve yields to meet food demands of increasing world populations. In the salt affected environment plants required to absorb essential nutrients from a dilute source in the presence of highly concentrated nonessential nutrients. Nutrient uptake and use efficiency in salt affected soils is low due to salt stress and negative interactions with cations and anions present in high concentrations. Hence, a higher amount of nutrients is necessary in salt affected soils compared to normal soils. Biological nitrogen fixation is also adversely affected in legumes grown on salt affected soils. Salts also reduce activity of many enzymes which supply energy for nutrient uptake. The important soils and plant management practices which can improve nutrient uptake and use efficiency in salt affected soils are use of soil amendments to reduce effect of salts, application of farmyard manures to create favorable plant growth environments, leaching salts from soil profile and planting salt tolerant crop species or genotypes within species. Addition of fertilizers, especially potassium may also help in reducing salinity effects and improving nutrient use efficiency.     

How good is the evidence that soil‐applied biochar improves water‐holding capacity?

Biochar application to soil is suggested as a way of enhancing soil fertility by increasing the availability of nutrients and water. The former is perhaps better documented while the latter has less experimental support. This review critically investigates the recent literature which focuses on determining whether biochar induces increases in plant available water and that this provides part of the explanation for possible increases in crop yield. A number of studies suggest that biochar increases crop yields, and this is linked to the enhancement of soil water content and increased crop growth. However, many of these studies fail to fully consider if the measured biochar increases of 10–30% in soil water content were actually responsible for an increase in plant available water for crop growth. There is also limited evidence of increased crop yields when biochar is used in field experiments. While biochar soil application may increase soil water content, this appears to most likely occur with free draining coarsely textured sandy soils. As yet there is limited evidence that biochar improves soil water content in temperate soils and even less that it facilitates plant tolerance to drought stress. More recent literature shows the use of methods which quantify soil biochar changes with respect to plant water availability. However, despite some advances in our understanding of biochar's mode of action, there are still only a few studies which link increases in plant available water with increased crop yields, and particularly with respect to the longer term use and functionality of soil‐applied biochar.     

离子交换树脂膜原位提取土壤有效性养分的探讨

连续3年在实验室内和利用盆栽及田间进行了利用离子交换树脂膜对土壤的原位测定,探讨影响树脂膜对土壤中养分离子吸收的因素;制定出一套包括树脂膜埋置方法,时间,洗脱土粒和解吸被树脂膜吸附的养分等的标准方法,称为植物根模拟法(Plant root simulator)。 通过400多个土壤样品测定结果表明,用树脂膜提取的土壤有效氮、磷、钾和硫与用常规化学法提出的量有显著相关。盆栽条件下,树脂膜原位埋置法对预测供试作物油菜吸取土壤有效养分的能力与常规化学法相当,而硫的效果更好。树脂膜在田间直接埋置能合理地表征土壤中硝态氮的分布,并具有同时提取土壤各种有效养分的能力。由于离子树脂交换膜不受地域影响,具有广泛的通用性,因而在土壤养分有效性的研究中应用前景广阔。     

Foliar Fertilization of Crop Plants

Essential plant nutrients are mainly applied to soil and plant foliage for achieving maximum economic yields. Soil application method is more common and most effective for nutrients, which required in higher amounts. However, under certain circumstances, foliar fertilization is more economic and effective. Foliar symptoms, soil and plant tissue tests, and crop growth responses are principal nutrient disorder diagnostic techniques. Soil applications of fertilizers are mainly done on the basis of soil tests, whereas foliar nutrient applications are mainly done on the basis of visual foliar symptoms or plant tissue tests. Hence, correct diagnosis of nutrient deficiency is fundamental for successful foliar fertilization. In addition, there are some more requirements for successful foliar fertilization. Foliar fertilization requires higher leaf area index for absorbing applied nutrient solution in sufficient amount, it may be necessary to have more than one application depending on severity of nutrient deficiency. Nutrient concentration and day temperature should be optimal to avoid leaf burning and fertilizer source should be soluble in water to be more effective. Foliar fertilization of crops can complement soil fertilization. If foliar fertilization is mixed with postemergence herbicides, insecticides, or fungicides, the probability of yield response could be increased and cost of application can be reduced.     

不同水分条件下表层施磷对小麦吸收下层土壤养分的影响

采用分层隔水盆栽试验,研究了不同土壤状况下,土壤表层(0-15cm)施磷对冬小麦吸收利用下层土壤(15-35cm)中氮磷钾养分影响。结果表明,土壤干旱降低了土壤磷、钾的有效性,严重抑制植株生长。不施磷肥时,小麦根系下扎量高于施磷处理,能从富含水分和养分的下层土壤吸收水分和养分,从而改善其营养状况,增加生物量。在土壤湿润的情况下,小麦对上层土壤养分的吸收量增加,同时由于扎入下层土壤中的根系量增多,因而也能利用一部分下层土壤中的氮磷钾养分。     

Visualization of root growth in heterogeneously contaminated soil using neutron radiography

We used neutron radiography (NR), a non-invasive and in situ technique, to study living plant roots in soil. Plant roots have a larger water content than their unsaturated surrounding media. As water strongly attenuates a neutron-beam, NR can identify root structures in detail. We investigated the use of NR to visualize the root growth of lupin in quartz sand and in a loamy sand field soil. Further experiments elucidated the root growth of lupin in the loamy sand heterogeneously contaminated with 10 and 20 mg kg⁻¹ boron (B) and 100 mg kg⁻¹ zinc (Zn). We obtained high-quality images of root growth dynamics in both media with a resolution range of 110–270 μm. The images with quartz sand revealed fine structures such as proteoid roots that are difficult to locate in situ by other methods without destruction of the soil. Though quartz sand provided excellent visibility of roots, it proved to be a poor medium for growing plants, probably because of its bulk density (1.8 Mg m⁻³). The images with field soil showed normal root growth with slightly less contrast than the quartz sand. The poorer contrast was due to the greater neutron interaction with soil water and soil organic matter. In the heterogeneously contaminated soil, root growth was significantly reduced in the contaminated part of the soil in all B and Zn treatments. This study shows that NR has potential as a non-invasive method to investigate root growth over time as well as the response of roots to various abiotic stress factors.     

Glacially abraded rock flour from Greenland: Potential for macronutrient supply to plants

Rock flour (RF) is a fine‐grained material produced naturally by glacial movement and resulting bedrock abrasion. In Greenland fluvial transported RF from the inland ice sheet sediments in riverbeds and marine outflows. This fine‐sized RF (50% < 9.8 µm) has a high reactivity and may therefore potentially be used to rejuvenate nutrient poor soils and provide nutrients to plants. The aim of this study was to evaluate the ability of a RF from Greenland to supply P, K, Mg, and S to plants. A double‐pot system was used, in which ryegrass (Lolium perenne L.) could take up nutrients from both a hydroponic solution and a soil‐compartment with or without RF amendment; a soil mixture or pure sand was used in the soil‐compartment to estimate RF‐soil interaction effects. Omission of single nutrients from the hydroponic solution allowed assessment of which nutrients the RF in the soil‐compartment was able to supply. Ryegrass biomass was harvested four times during 62 days. We found that RF could supply K continuously to plants grown in soil or sand, but insufficient to fully circumvent K deficiency. During 62 days 5.8% and 4.3% of the applied K from RF was accumulated in the aboveground plant tissue in soil and sand, respectively. Mg was supplied from RF to plants in sand, but no significant effects were observed in soil, possibly due to background soil Mg availability. The amounts of P and S supplied to plants were insignificant. These results indicate the potential of Greenland RF to act as a slow release K and Mg fertilizer.     

Effects of weathering state of coarse‐soil fragments on tree‐seedling nutrient uptake

Fine earth accumulated within the weathering fissures of the coarse‐soil fraction (particles > 2 mm), so called “stone‐protected fine earth”, can provide a high short‐term nutrient release by cation exchange. It is thus hypothesized that unweathered gneiss particles cannot provide plants with exchangeable‐cation nutrients and that biological weathering is needed to include silicate‐bound nutrients into biochemical cycles. In a microcosm experiment, ectomycorrhizal Norway spruce (Picea abies) seedlings were grown on either weathered or unweathered paragneiss coarse‐soil fragments under natural hydraulic and climatic boundary conditions. A nutrient solution containing N, P, and K was added, however Mg and Ca could only be taken up from the coarse‐soil substrate. Solutes in drainage were analyzed during the experiment; plant nutrient uptake was determined after the experiment ended. Solute dynamics depended on the weathering state of the substrates: unweathered gneiss showed high initial Mg and Ca fluxes that diminished strongly afterwards, whereas weathered gneiss showed a much more gradual and sustainable release of these cations. Patterns in dissolved organic C and sulfate drainage indicated that the internal pores of weathered gneiss fragments contained organic material most likely as a result of living spaces from microorganisms. Plant biomass did not differ between treatments, however Mg content was higher in seedlings grown on weathered gneiss. Nutrient budgets demonstrated that the “stonesphere” of weathered gneiss can act as a quasi‐constant nutrient source whereas unweathered gneiss only provided high initial nutrients fluxes. In nutrient‐depleted, acidified fine‐earth environments, the coarse‐soil fraction may therefore act as a retreat for nutrient‐adsorbing tissues and as a buffer for nutrient shortages.     

Coupling of nutrient cycling and carbon dynamics in the Arctic,integration of soil microbial and plant processes

Most studies of nutrient cycling in arctic ecosystems have either addressed questions of plant nutrient acquisition or of decomposition and mineralization processes, while few studies have integrated processes in both the soil and plant compartments. Here, we synthesize information on nutrient cycling within, and between, the soil/microbial and the plant compartments of the ecosystems and integrate the cycling of nutrients with the turnover of organic matter and the carbon balance in tundra ecosystems. Based on this compilation and integration, we discuss implications for ecosystem function in response to predicted climatic changes.Many arctic ecosystems have high amounts of nutrients in the microbial biomass compared to the pools in the plant biomass both due to large nutrient-containing organic deposits in the soil and low plant biomass. The microbial pools of N and P, which are the most commonly limiting nutrients for plant production, may approach (N) or even exceed (P) the plant pools. Net nutrient mineralization is low, the residence time of nutrients in the soil is long and the nutrients are strongly immobilized in the soil microorganisms. This contributes to pronounced nutrient limitation for plant productivity, implies that the microbial sink strength for nutrients is strong and that the microbes may compete with plants for nutrients, but also that they are a potential source of plant nutrients during periods of declining microbial populations. The extent of this competition is poorly explored and it is uncertain whether plants mainly take up nutrients continuously during the summer when the microbial activity and, presumably, also the microbial sink strength is high, or whether the main nutrient uptake occurs during pulses of nutrient release when the microbial sink strength declines.Improved knowledge of mechanisms for plant-microbial interactions in these nutrient-limited systems is important, because it will form a basis also for our understanding of the C exchange between the ecosystems and the atmosphere under the predicted, future climatic change. High microbial nutrient immobilization, i.e. low release of plant-available nutrients, paired with high microbial decomposition of soil organic matter will lead to a loss of C from the soil to the atmosphere, which may not be compensated fully by increased plant C fixation. Hence, the system will be a net source of atmospheric C. Conversely, if plants are able to sequester extra nutrients efficiently, their productivity will increase and the systems may accumulate more C and turn into a C sink, particularly if nutrients are allocated to woody tissues of low nutrient concentrations.     

DOES NITROGEN FERTILIZATION ENHANCE DROUGHT TOLERANCE IN SUNFLOWER? A REVIEW

Water stress is one of the major limitations to the agricultural productivity around the globe, particularly in warm, arid and semi-arid regions of the world. Sunflower (Helianthus annuus L.), being a crop with medium water requirements, has the ability to tolerate a short period of drought. However, water stress in the soil as well as inside the plant influences various physiological and biochemical processes. This may inhibit plant growth, decrease developmental activities of the cells and tissues and cause a variety of morphological, physiological and biochemical modifications. Nitrogen (N) is one of the most important mineral nutrients because of its numerous effects on plant growth and yield. A number of fundamental processes such as water and nutrient uptake, protein metabolism, photosynthesis, carbon partitioning, and enzyme and plant hormonal activities are regulated by N. These responses result in profound changes in growth rate, net photosynthate production, plant development, and yield. It is well documented that nutrient uptake of plants is inhibited in dry soils and with expected nutrient deficiencies the normal functioning of the plants is affected. Different strategies are being practiced in the world to cope with the problem of nutrient deficiency under water stress. Nitrogen application either through soil or through foliar feeding is an important strategy to alleviate the adverse effect of drought. Supplemental application of N as foliar fertilization to soil-applied fertilization is important in situation where nutrient supply through soil is limited. Some of the relevant work available about the effect of water stress and nutrient availability in sunflower is reviewed in this paper.     

Hydraulic properties of Eucalyptus grandis in response to nitrate and phosphate deficiency and sudden changes in their availability

Some herbaceous plant species have been shown to dynamically alter the hydraulic properties of their roots in response to sudden changes in the concentrations of mobile nutrients. These hydraulic adaptations effectively allow plants to ‘chase' mobile nutrients across the rhizosphere. Trees, on the other hand, could mitigate effects of heterogeneous, dynamic soil environments with extensive root systems as such systems would effectively equalize nutrient availability. In addition, large dendritic root systems would reduce the effectiveness of rapid, localized, physiological hydraulic changes as these local changes might cancel each other at lower‐order root junctions. Thus, the aim of this study was to determine if trees (Eucalyptus grandis) employ short‐term (minutes to hours), physiological hydraulic changes or rely on long‐term (days), growth‐based hydraulic acclimations to enhance mobile nutrient uptake. We used two nutrients, nitrogen (N) and phosphorus (P), that are characterized by contrasting soil mobility: N being mobile and P immobile. Transpiration, whole‐plant hydraulic resistance (liquid phase), and the hydraulic resistance of single roots of E. grandis plants grown in high and low N combined with high and low P availability were measured. In general, plants grown with high N availability had lower whole‐plant hydraulic resistance than plants grown with low N availability. When N or P were in short supply, a sudden addition of N or P did not change either single‐root or whole‐plant hydraulic resistance at a given leaf water potential. However, addition of N reduced the transpiration rate, thus, enhancing plant water status, suggesting that E. grandis behavior prioritizes water conservation over N uptake in short‐term. Prolonged exposure to low nutrient availability resulted in high overall hydraulic resistance further suggesting prioritization of water conservation over N gain.