土壤中可溶性氮和pH对有机肥和化肥的短期响应

通过温室盆栽试验,施用猪粪堆肥(PC)、污泥堆肥(SC)、菜籽饼肥(CM)和无机化肥(IF)后,对土壤pH值和不同形态氮素的短期响应进行研究,探讨土壤可溶性无机氮、有机氮及土壤pH值之间的相互关系。结果表明,CM,PC和IF处理的pH值都呈现先快速下降后缓慢回升的趋势,但PC处理下降幅度较小;而SC处理和不施肥对照(CK)处理的pH值基本不变;不同施肥处理均提高了土壤中氮素水平,3种有机肥中以CM处理最显著地提高了土壤中总可溶性氮(TDN)、NH4+-N、NO3--N和可溶性有机氮(DON)浓度,PC处理的次之,SC处理的最弱,而IF处理与CM处理提高幅度相似。不同施肥处理中土壤可溶性氮表现出不同的短期响应,IF处理和CM处理的TDN分别经过11,13d的稳定期后迅速上升到一个较高的水平,至31d开始下降;PC处理和SC处理未出现突然升高的现象,而是前20d较稳定在一定的范围内波动,之后缓慢下降,总体与对照趋势一致。不同处理土壤中NH4+-N均呈下降趋势;IF处理、CM处理的NO3--N呈增加趋势,PC处理的呈先增加后降低趋势,而SC处理的呈缓慢下降趋势,结果说明不同有机肥在土壤中的转化存在明显的差异。相关性分析显示,CM处理、IF处理的土壤pH值与NH4+-N呈极显著正相关,而与NO3--N呈极显著负相关;除PC处理外,其他处理的DON含量与TDN具有显著正相关性,而PC处理的DON同时与NH4+-N、NO3--N呈显著负相关。     

长期施肥对棕壤铁形态及其有效性的影响

【目的】利用沈阳农业大学棕壤肥料长期定位试验,研究不同施肥处理对耕层土壤酸碱度（pH）、氧化还原电位（Eh）和有机质的影响,探讨不同施肥条件下土壤游离态氧化铁、无定形氧化铁、亚铁总量和有效铁含量的变化以及与pH、Eh和有机质的关系。 【方法】本文选取试验处理为CK（不施肥）、N（氮肥）、NP（氮磷肥配施）、NPK（氮磷钾肥配施）、M（有机肥）、MN（有机肥与氮肥配施）、MNP（有机肥与氮磷肥配施）、MNPK（有机肥与氮磷钾肥配施）。在2014年大豆收获期,采集了不同施肥处理0-20 cm耕层土壤样品,分析了土壤pH、Eh、有机质含量以及游离态氧化铁、无定形氧化铁、亚铁和有效铁含量。 【结果】与1979年原始土壤相比,所有处理土壤pH显著降低了0.6~1.4个单位。不施肥处理土壤有机质下降了11.5%,化肥处理有机质略有下降,而有机肥处理有机质含量显著增加。所有处理有效铁含量显著增加,化肥有机肥配施增加幅度更大;与2014年不施肥处理相比,施用氮肥处理土壤pH最低,而氮肥配施磷、钾和有机肥中的一种或几种pH又有所上升,其中配施有机肥效果最明显;施用化肥处理土壤Eh增加,而施用有机肥处理Eh则下降。施用化肥土壤游离态氧化铁和有效铁含量增加,亚铁含量下降,而施用有机肥土壤游离态氧化铁降低,亚铁总量及有效铁含量增加。 【结论】经过长期耕作和施肥,土壤pH显著下降,有效铁含量显著增加。长期不施肥土壤有机质显著下降。施用氮肥土壤酸化趋势明显,磷、钾和有机肥配施能够缓解氮肥引起的酸化现象。施用有机肥显著降低土壤氧化还原电位,有利于氧化态铁向还原态铁转化,更有利于增加土壤有效性铁的含量。     

有机肥和化肥长期施用对土壤活性有机氮组分及酶活性的影响

本文以中国农业科学院山东禹城长期定位施肥试验为平台,研究了长期施用有机肥和化肥26年后对土壤活性氮库不同组分［颗粒有机氮（POM-N）、 可溶性有机氮（DON）、 微生物量氮（SMBN）及轻组有机氮（LFOM-N）］及土壤酶活性的影响。结果表明,与不施肥相比,长期施肥显著提高了土壤全氮、 颗粒有机氮、 可溶性有机氮、 微生物量氮以及轻组有机氮的含量,长期施有机肥效果好于化肥,施用高量有机肥效果好于施用常量有机肥。常量施用量下,50%有机肥和50%化肥配施处理其土壤全氮和活性有机氮库各组分含量与高量化肥处理的相当。长期施化肥处理土壤全氮及活性有机氮库各组分含量随施肥量的增加而显著增高。POM-N对土壤全氮的贡献率最高,且明显受施肥方式的影响,LFOM-N对土壤全氮的贡献率不随施肥方式的改变而变化。长期施肥处理土壤脲酶、 碱性磷酸酶和蔗糖酶活性显著增加,它们之间及与土壤全氮、 速效磷及有机碳含量间呈现显著或极显著相关性,脲酶活性与土壤各活性氮组分间也存在显著或极显著相关性; 但长期施肥后土壤过氧化氢酶的活性低于不施肥     

施肥对生土地谷子根苗生长及根际土壤酶和微生物种群的影响

以黄土母质性生土为供试土壤,采用盆栽试验,探讨了氮、磷、钾及有机肥对谷子生长发育的影响。结果表明:(1)磷是生土地土壤肥力形成与作物生长发育以及根际土壤酶系统和微生物系统形成的首要原始起动营养元素;(2)含磷的处理组合,谷子幼苗阶段地上下部生长均明显较不施磷的长势强,而单施氮和钾甚至不及不施肥的对照。氮、磷、钾对谷子根苗生长的影响顺序为磷氮钾;(3)进入谷子中后期生长阶段,氮素的作用,特别是氮、磷、钾对谷子株高及地上部干重的综合效果表现明显;(4)产量表现为含磷的处理组合,产量及产量结构均明显提高;(5)单施优质高量的有机肥对生土地根苗生长与产量形成具有当年立竿见影的效果;(6)发达的根系促进了根际微生物群落的迅速繁衍,提高了根际土壤酶的活性,有益于促进根-土系统向着熟化方向发展,使生土地上的谷子当年获得正常产量。     

Responses of soil microarthropods to inorganic and organic fertilizers in a poplar plantation in a coastal area of eastern China

Soil microarthropod community is an essential functional unit of soil food webs. Fertilizers can induce an alteration of quantity and quality of food for soil fauna and trigger profound changes in soil faunal communities. We initiated this study to examine the influence of organic and inorganic fertilizers on soil microarthropods in poplar plantations (Populus deltoides) in a coastal region of northern Jiangsu, eastern China. We established a control and four fertilizer application treatments: low and high levels of organic fertilizers, low and high levels of inorganic fertilizers. Organic fertilizer amendments increased both soil organic carbon (C) and total nitrogen (N), whereas inorganic fertilizer had a positive significant effect on soil total N. The application of both inorganic and organic fertilizers resulted in significantly reduced soil pH. We found that both inorganic and organic fertilizers increased the abundance of all soil microarthropods, bacterivorous Acari, and hemiedaphic and epedaphic Collembola, but had no influence on the total taxonomic richness, Shannon diversity index and DG diversity index of the microarthropod community. The abundance of soil microarthropods was positively correlated with soil C and N, and negatively with pH. Our results indicate that changes in the quality and quantity of soil organic matter and other immediate chemical properties after fertilizer application can increase the abundance of soil microarthropods, but have a limited influence on their diversity in the coastal alkaline soils of eastern China.     

有机肥施用对植烟土壤氮素矿化及土壤酶和微生物群落的影响

为探索不同有机肥对植烟土壤氮素矿化及土壤微生态的影响。在等氮(100 mg/kg)投入和30℃条件下对施入不同有机肥(植物源有机肥、芝麻饼肥、生物质炭有机肥)的土壤进行室内培养，分析不同时期土壤NO3–-N和NH4+-N含量、土壤酶活性及细菌群落多样性的变化。结果表明：施用不同有机肥均可提高土壤矿质氮含量，在培养前期以饼肥矿化量最高，矿化速率最快，而后期为生物质炭有机肥处理的氮素矿化量、矿化速率高于其他处理；施用生物质炭有机肥、植物源有机肥均可显著提高土壤脲酶、硝酸还原酶活性，芝麻饼肥对蛋白酶、蔗糖酶活性有显著的提升作用；相较不施有机肥处理，添加不同有机肥的土壤细菌多样性明显提升。优势菌门为变形菌门、酸杆菌门、拟杆菌门、浮霉菌门，优势菌属为Sphingomonas、RB41,Sphingomonas在不施有机肥处理中最高，RB41在生物质炭有机肥处理中最高。Sphingomonas与蔗糖酶活性呈正相关关系，与脲酶、硝酸还原酶均呈负相关关系；PCA分析显示，生物质炭有机肥处理的细菌群落结构与其他处理相比差异较大。施用芝麻饼肥有利于促进早期氮素矿化，提高矿化量，而生物质炭有机肥后期矿化量较...     

Effect of Rapeseed Green Manure Amendment on Soil Properties and Rice Productivity

Green manure crops can be used as alternatives to mineral fertilizers because they improve soil structure and nutrient content of soil. Here, we evaluated the effect of partial replacement of mineral nitrogen (N) fertilizer with rapeseed residue green manure on soil properties and rice productivity in a rice–rapeseed double-cropping system. The treatments were comprised of four levels of mineral N fertilizer and recommended levels of phosphate and potash fertilizers. Rice clum and panicle length did not differ between treatments. However, the proportion of ripened grains and 1,000-grain weight of rice plants were greater under rapeseed residue amendments than those under 100% conventional mineral N fertilizer treatments. Paddy soils treated with rapeseed residues had greater soil organic matter and exchangeable cations than those treated with mineral N fertilizer alone. Thus, rapeseed residues applied as green manure can serve as alternative N sources in sustainable rice–rapeseed double-cropping systems.     

长期施肥对棕壤氧化还原酶活性和动力学特征的影响

土壤质量与土壤的生物学性质密切相关,施肥制度对土壤生物学特性的影响研究对土壤质量管理具有重要的意义。本文以沈阳农业大学长期肥料定位试验为基地,研究长期不同施肥制度对土壤理化性质,过氧化氢酶、脱氢酶活性和动力学特征的影响。主要研究结果:施用化肥、有机肥配施化肥降低土壤pH值,提高4种土壤全量养分(全C、全P、全S、全N)含量,有机肥配施化肥提高土壤速效N含量;低量化肥配施有机肥显著提高土壤过氧化氢酶活性及Vmax/Km值和Vmax值,低量化肥配施有机肥显著提高脱氢酶活性及Vmax/Km值,降低脱氢酶Km值。本文的研究表明,合理的化肥施用量及配施有机肥是提高土壤生物学活性的有效手段。     

Effects of mineral and biofertilizers on barley growth on compacted soil

Abstract

Biofertilizers are an alternative to mineral fertilizers for increasing soil productivity and plant growth in sustainable agriculture. The objective of this study was to evaluate possible effects of three mineral fertilizers and four plant growth promoting rhizobacteria (PGPR) strains as biofertilizer on soil properties and seedling growth of barley (Hordeum vulgare) at three different soil bulk densities, and in three harvest periods. The application treatments included the control (without bacteria inoculation and mineral fertilizers), mineral fertilizers (N, NP and P) and plant growth promoting rhizobacteria species (Bacillus licheniformis RC04, Paenibacillus polymyxa RC05, Pseudomonas putida RC06, and Bacillus OSU-142) in sterilized soil. The PGPR, fungi, seedling growth, soil pH, organic matter content, available P and mineral nitrogen were determined in soil compacted artificially to three bulk density levels (1.1, 1.25 and 1.40 Mg m^?3) at 15, 30, and 45 days of plant harvest. The results showed that all the inoculated bacteria contributed to the amount of mineral nitrogen. Seed inoculation significantly increased the count of bacteria and fungi. Data suggest that seed inoculation of barley with PGPR strains tested increased root weight by 9–12.2%, and shoot weight by 29.7–43.3% compared with control. The N, NP and P application, however, increased root weight up to 18.2, 25.0 and 7.4% and shoot weight by 31.6, 43.4 and 26.4%, respectively. Our data show that PGPR stimulate barley growth and could be used as an alternative to chemical fertilizer. Soil compaction hampers the beneficial plant growth promoting properties of PGPR and should be avoided.     

有机肥替代部分化肥对滨海盐碱地土壤改良和小麦产量的影响

合理利用有机肥资源,将有机肥替代部分化肥是实现减肥目标的重要技术途径之一。特别对于盐碱化土壤,有机肥替代部分化肥既能减少化肥的使用又能改善土壤的理化性质,促进作物产量的提高。本文以滨海盐碱地为研究对象,2014—2016年连续两年在滨州市无棣县渤海粮仓试验基地通过大田试验,研究了不施肥(CK)、普通化肥(CCF)、有机肥替代低量化肥(LOM)、有机肥替代中量化肥(MOM)、有机肥替代高量化肥(HOM)5种施肥模式对盐碱地土壤改良和小麦产量的影响。结果表明:与CK相比,CCF处理对盐碱地土壤盐分的影响不大,而有机肥替代部分化肥处理显著降低了土壤水溶性盐总量和pH,特别是在小麦开花期,MOM和HOM处理明显改善了土壤盐碱化,显著降低了土壤中水溶性钠和交换性钠的比例,使ESP和SAR值减小,其中以HOM处理对滨海盐碱土的土壤盐分改良效果最佳;与CK相比,各施肥处理的土壤养分含量均有提高,有机肥替代处理与CCF相比,对土壤全氮和有效磷的含量影响不明显,而HOM处理的速效钾含量显著高于其他处理,在一定程度上抑制了Na的毒害,有机肥替代处理还显著提高了土壤有机质的含量,改善了土壤环境;从CK到HOM,小麦产量依次递增,与CCF相比,有机肥替代处理LOM、MOM、HOM分别增产7.5%、18.8%、26.4%。综上,有机肥替代部分化肥,达到了减肥的目的,并对滨海盐碱地有明显的改良效果,提高了小麦产量。且在3个有机肥替代部分化肥的施肥处理中,以HOM施肥处理对滨海盐碱地的改良效果最优,获得的小麦产量最高。     

不同有机肥处理对黄冠梨生长及果园土壤性状的影响

为探明不同有机肥对梨树生长和果园土壤生物性状的影响,通过田间小区试验研究了不施肥处理(CK)、传统施肥(TF)、生物有机肥(BOF)、鸡粪堆肥(CM)及有机无机复混肥(CF)对黄冠梨树地上部生长、产量和品质、土壤微生物量碳氮和矿质态氮含量动态变化的影响.结果表明,(1)各处理均增加了叶面积、百叶重及新梢长度,果实可溶性固形物、可溶性糖含量和糖酸比也有不同程度提高,其中BOF处理效果最好,与CK相比BOF处理单果重和产量分别增加了17.4％和33.9％;(2)与CK和TF相比,其它有机肥处理在整个生育期均增加了土壤微生物数量及微生物量碳氮含量,且两者呈先增加后下降趋势;(3)梨园土壤中的矿质态氮以硝态氮为主,从萌芽期至二次膨大期各施肥处理矿质态氮含量逐渐下降,降幅为78.9％～88.7％,成熟期BOF处理的土壤矿质态氮含量高于其它处理.可见,施用有机肥可以促进树体生长,提高土壤微生物数量,改善土壤供氮特性.     

长期不同施肥条件下土壤微生物量及 土壤酶活性的季节变化特征

研究长期不同施肥条件下褐潮土微生物量碳（SMBC）、微生物量氮（SMBN）和土壤酶活性随季节的变化特征。结果表明,长期施肥条件下土壤SMBC、SMBN含量及土壤酶活性均表现出一定的季节变化。SMBC、SMBN含量在各施肥处理中的顺序为:化肥与猪厩肥配施处理（NPKM）化肥配施玉米秸秆处理（NPKS）单施化肥处理（NPK）不施肥处理（CK）,各处理之间差异显著(P0.05);施肥还显著提高了土壤脲酶、转化酶、碱性磷酸酶活性,有机无机配施的高于单施化肥的。除过氧化氢酶活性随季节变化显著下降外,SMBC、SMBN、酶活性的值一般在夏季（6月到8月）较高。通过双因素单变量方差分析表明,不同施肥制度与季节变化对SMBC、SMBN与酶活性的影响分别达极显著水平（P0.01）,不同施肥制度的SMBC、SMBN与酶活性的季节波动有极显著不同（P0.01）。     

水生植物堆肥替代部分氮肥提高水稻产量与稻田土壤肥力

为评价太湖流域水生植物堆肥对水稻产量及稻田土壤肥力效应,在太湖流域典型稻田连续进行4a的田间定位试验,比较在等氮条件下不同比例的水生植物有机堆肥替代处理(有机氮替代率分别为0、20％、40％、60％、80％和100％)引起的水稻籽粒产量、产量构成因子、氮磷钾吸收量以及土壤碳氮含量和pH值变化.结果表明:与单施尿素相比,水生植物有机堆肥与尿素配施利于水稻产量的提高,并随着有机肥替代率增加,水稻产量呈先增后降;当有机肥替代率达40％和60％时产量最高.单施有机肥和单施尿素处理水稻籽粒产量相当.单施有机肥显著降低了有效穗数,有机肥和尿素配合施用则可减轻甚至消除这一效应;有机肥替代率在40％和60％时,有效穗数、穗粒数和结实率均较高.随着有机肥施用量增加,水稻秸秆氮浓度降低,籽粒氮浓度无影响;水稻磷浓度和吸收量均无显著差异;有机肥与尿素配施均显著提高了秸秆钾吸收量,有机肥替代率在80％时可显著提高籽粒钾吸收量.表层土壤全氮和有机碳含量及土壤pH值均与有机肥替代率呈显著正相关关系.有机肥-尿素配施处理下土壤全氮和有机碳均较4a前显著提高.有机肥替代率为80％和100％,土壤pH值较试验前土壤分别显著升高.由此可见,水生植物有机肥与尿素配施可以提高太湖稻作区水稻产量,增加土壤有机质含量和减缓土壤酸化程度,可作为太湖稻作区一项环保型施肥技术.     

有机养分替代对小麦产量、土壤肥力及麦田氮磷径流流失的影响

为探讨不同有机肥替代率对小麦减肥增效及减少麦田氮磷流失的效果,在等氮有机养分替代条件下进行田间小区监测试验,设置8种不同处理,分别为空白对照(CK),常规施肥(CF),100%、50%、30%猪粪有机养分替代氮肥(M1、M2、M3)及100%、50%、30%秸秆有机养分替代氮肥(F1、F2、F3),研究有机肥替代率对小麦产量、麦田氮磷流失、肥料利用率和土壤肥力的影响。结果表明,100%有机养分替代处理与常规施肥处理相比小麦产量均显著降低,但减少了麦田氮径流流失,提高了土壤肥力。麦田不同处理下总氮流失量为21.90~33.66 kg·hm^-2,与常规施肥处理相比,不同比例猪粪、秸秆有机养分替代处理总氮流失量减少了8.44%~25.94%;总磷流失量为0.60~2.00 kg·hm^-2,100%有机养分替代处理相比于常规处理总磷流失量升高了24.64%~44.93%。不同处理下小麦氮、磷肥利用率分别为17%~35%、5%~19%,其中30%~50%猪粪有机养分替代处理下氮、磷肥利用率较高,而100%猪粪、秸秆有机养分替代氮、磷肥利用率较常规施肥处理均显著降低。有机养分替代能够缓解土壤酸化,使土壤pH值维持稳定,与常规施肥处理相比,有机养分替代处理下土壤肥力有所提高。综合不同比例猪粪、秸秆有机养分替代对小麦产量、土壤肥力及麦田氮磷径流流失的影响,30%~50%猪粪有机养分替代在保证小麦高产稳产的同时,能有效降低麦田氮径流流失量,且维持较低水平磷径流流失量,是一种适宜的资源有效利用、节肥增效的有机养分替代模式。本研究结果为小麦生产中合理利用养分资源、减少化肥投入、控制麦田氮磷径流流失提供了参考。     

Ammonia oxidizer abundance in paddy soil profile with different fertilizer regimes

Studies about ammonia-oxidizing bacteria (AOB) and archaea (AOA) are often focused on topsoil, but little is known about their activity and distribution in subsoil. A long-term fertilizer experiment was conducted to assess the effects of different fertilizer treatments on AOB and AOA in vertical soil profiles of paddy soil plots that received no nitrogen fertilizer control (CK), NPK chemical fertilizers (CF), organic–inorganic mixed fertilizer (OIMF) and organic fertilizer (OF). Soil properties, potential nitrification rate (PNR) and amoA gene abundance of AOB and AOA were measured and analyzed by two-way ANOVA and correlation analysis. Quantitative PCR analysis of amoA genes showed that AOA were more abundant than AOB in all the soil samples. AOB declined sharply with soil depth. Compared with CK and OF treatments, CF and OIMF treatments had higher abundance of AOB throughout the soil profiles. However, AOA tend less responsive to soil depth and fertilizers compared to AOB. This caused the AOA/AOB ratios in subsoil higher than in topsoil, and in CK and OF higher than in CF and OIMF treatments. These results suggest that AOA are more abundant and can be better adapted to nutrient-poor subsoils than AOB, and autotrophic nitrification could likely be determined by a complex suite of environmental factors in vertical profiles of the paddy soil tested.     

The role of non‐crystalline Fe in the increase of SOC after long‐term organic manure application to the red soil of southern China

Because of the important role of soil organic carbon (SOC) in nutrient cycling and global climate changes, there has been an interest in understanding how different fertilizer practices affect the SOC preservation and promotion. The results from this study showed that long‐term application of manure (21 years) could increase significantly the content of SOC, total nitrogen (N) and soil pH in the red soil of southern China. The chemical structure of SOC was characterized by using solid‐state cross‐polarization magic angle spinning (CPMAS) ¹³C nuclear magnetic resonance (NMR) spectroscopy, and the aromatic C, ratio of alkyl C : O‐alkyl C, aromaticity and hydrophobicity of mineral fertilizers N, P and K plus organic manure (NPKM) and organic manure (M) treatments were less than those of mineral fertilizer nitrogen (N) and mineral fertilizers N, P and K (NPK) treatments. Both poorly crystalline (Fe_o) and organically complexed (Fe_p) iron contents were influenced significantly (P < 0.05) by different fertilizers, and it was observed that NPKM and M treatments increased the non‐crystalline Fe (Fe_o‐Fe_p) content. There was a significant (P < 0.01) positive correlation between soil organic C and non‐crystalline Fe in both the surface (0–20 cm) and subsurface (20–40 cm) soils. The results suggested that non‐crystalline Fe played an important role in the increase of SOC by long‐term application of organic manure (NPKM and M) in the red soil of southern China.     

Effects of mineral and organic fertilizers on the microfauna in a high-altitude reafforestation trial

Summary Protozoa (testate amoebae, ciliates), small metazoa (rotifers, nematodes), and soil enzymes (catalase, cellulase) were investigated in a reafforested fertilized site at the alpine timberline. Side-dressings of mineral and organic fertilizers were applied alone (90 g NPK; 90, 180, 300, and 450 g dried bacterial biomass per spruce seedling) and in combination with magnesite (90 g NPK+300 g Mg; 90, 180, and 300 g bacterial biomass + 300 g Mg each; 30 g dried fungal biomass + 270 g Mg). One-third of each quantity was applied in 1986, and two-thirds in 1988. None of the treatments caused a significant decrease in the biological parameters investigated in comparison with untreated controls. The soil life was more or less stimulated depending on the amount of organic material contained in the fertilizers and the quantity; 180–270 g organic material per seedling proved to be the most effective. Dried bacterial biomass increased the pH by about 0.5 units, catalase activity by about 70%, and the number of ciliates and nematodes by 150–400%. The ciliate biomass and the number of ciliate species were likewise increased. The organomineral fertilizers increased pH by up to two units and also stimulated the soil life, but where the organic content was less than 180 g per seedling, efficiency decreased markedly. The least biological activity was observed in the control soil and in soil fertilized with NPK. Testaceans, rotifers, and cellulolytic activities were only slightly (insignificantly) affected by the treatments. A pooled evaluation of the data (organic versus organomineral versus mineral treatments) and community analyses showed that the organic fertilizer caused a more pronounced increase in the soil life and greater changes in community structure than the mineral combinations. Two years after application of the fertilizers, the differences between the treatments and the unfertilized controls had diminished.     

Impact of fertilizers on total microbiological flora in planted and unplanted soils of long-term fertilization experiment

Microbial population both in diversity as well as numbers in soil is influenced by the amount and type of various compounds entering soil through plant litter, root exudates and management factors like mineral and organic fertilizers under various climatic conditions. This in turn affects crop production and sustainability of soil health. Therefore, we tested total and number of various groups of microflora and also substrate use by Biolog to assess the metabolic capabilities of various groups of microbes from soils of long term fertilization experiment, Halle (Germany) having different mineral and organic fertilizer treatments in unplanted and planted soils with two crops viz., Secale cereale and Medicago sativa . High number of total microorganisms, nitrogen fixers and phosphate solubilizers were observed in the rhizosphere of Medicago sativa and Secale cereale compared to bulk soils. Microbial counts were specially higher in treatments of NPK+FYM (farmyard manure) and NPK in Secale. Medicago had more P solubilizers in yearly addition of phosphatic fertilizers compared to three yearly treatment. During substrate utilization test (Biolog) maximum utilization was found for sugars followed by amino acids in Secale whereas in Medicago sugars were followed by carboxylic acids.     

长期单施不同量化肥和有机肥后盐化潮土pH和EC的变化

【目的】研究长期单施不同量有机肥和化肥对华北平原盐化潮土区土壤pH和EC的影响,以期为本地区科学实施有机肥替代化肥提供数据支撑和理论依据。【方法】本研究以始于2006年的长期定位试验为研究平台,在冬小麦–夏玉米一年两熟种植制度下,有机肥和化肥处理均设置10个氮水平,N 0、60、120、180、240、300、360、420、500和600 kg/hm2,共计19个处理,监测了连续施用不同量化肥和有机肥10年共20季作物后潮土的EC和pH值。【结果】单施化肥N 60、120、180、240 kg/hm2的土壤EC分别为232、280、220和258 μS/cm,与不施肥处理CK的243 μS/cm差异不显著 (P > 0.05);化肥氮量为N 300、360、420、500和600 kg/hm2处理,土壤EC分别为320、403、513、516和570 μS/cm,显著高于CK和N 60～240 kg/hm2处理 (P < 0.05)。10年20季播种作物后,不施肥处理土壤pH基本在7.97上下波动,化肥处理N从60增加到600 kg/hm2,土壤pH分别为7.96、7.89、7.88、7.85、7.83、7.72、7.53、7.51和7.42,随着肥料用量的增加呈显著降低趋势 (P < 0.05)。肥料类型对黄淮海地区盐化潮土土壤EC和pH存在显著影响。化肥氮量为N 60、120、180、240 kg/hm2处理与有机肥氮量N 60、120、180、240处理土壤EC和pH类似,分别相差为9.90、–15.9、26.4、1.76 μS/cm和 –0.04、0、0.03、0.02,差异均未达显著水平,但随着肥料投入量的增加,差距越来越明显,且与施肥量呈显著线性相关 (P < 0.05)。化肥N 300、360、420、500、600 kg/hm2处理比同量有机肥氮处理的土壤EC值分别高42.1、90.5、195、110、188 μS/cm,土壤pH分别低0.08、0.09、0.18、0.18、0.14个单位,差异达显著水平 (P < 0.05)。【结论】在华北盐化潮土上,化肥和有机肥氮投入量不超过240 kg/hm2范围内,连续施用10年20季作物后,均未发现对土壤EC值和pH有不利影响,施化肥和有机肥的土壤EC值和pH差异不显著。然而,氮投入量超过300 kg/hm2后,等养分化肥土壤EC值显著高于有机肥,pH显著低于有机肥。因此,要提高盐化潮土的养分投入,应控制化肥用量,依靠有机肥投入,维持土壤pH和土壤EC的稳定。     

半干旱农田生态系统作物根系和施肥对土壤微生物体氮的影响

盆栽和大田试验表明,作物根系显著影响土壤微生物体氮的含量。在田间试验条件下,根际土壤微生物体氮比非根际土壤平均高出N54.7μg/g;盆栽试验中,根际土壤微生物体氮平均含量为N77.1±13.6μg/g,而非根际土壤为N65.2±17.0μg/g,差异达显著水平,根际微生物体氮含量为非际根际土壤的1.10～2.04倍。施肥能明显增加土壤微生物体氮含量,但影响程度因肥料种类而不同。秸秆和富含有机物质的厩肥对土壤微生物体氮的影响远大于化学肥料,而且土壤微生物体氮含量随秸秆施用量增加而增加。在红油土上进行的20年长期田间定位试验结果表明,对不施肥和施氮磷处理,0—20cm土层的微生物体氮分别是N102.2和110.4μg/g;在施氮磷的基础上,每公顷配施新鲜玉米秸秆9375kg、18750kg、37500kg和厩肥37500kg时,相应土层微生物体氮分别是N147.5、163.2、286.4和265.3μg/g。培养条件下,当有效能源物质缺乏时,微生物对NH4+-N的同化固定能力远大于NO3--N,但在加入有效能源物质葡萄糖后,微生物对2种形态氮的固定量大幅度增加,且对2种形态氮的固定量趋于一致。