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球孢白僵菌脂肪酸、酯酶、脂肪酶及其与毒力的关系 总被引:7,自引:1,他引:7
研究12株球孢白僵菌脂肪酸、酯酶和脂肪酶的产生水平及菌株对马尾松毛虫毒力间的关系。结果表明:酯酶产生水平(E)、脂肪酶产生水平(L)及豆蔻酸百分含量(X)等与白僵菌菌株对马尾松毛虫的毒力(LT50)间存在一定的相关性,其相关方程分别为:LT50=2.0931/(lgE-5.1165)+0.4091;LT50=-6.68L^2+31.9886L;LT50=13.5192-8.7042X。 相似文献
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白僵菌胞外蛋白酶的测定及其与酯酶型的关系 总被引:14,自引:0,他引:14
用明胶琼脂培养法和营养液振荡培养法测定了白僵菌不同酯酶型菌株的胞外蛋白酶,并用前一种方法测定了同一酯酶型菌株的胞外蛋白酶。发现不同类型菌株间和同一类型菌株内个体间胞外蛋白酶产生水平都有差异,少数几株差异显著。测定蛋白酶方法不能单独用来鉴别菌株。明胶琼脂培养法比营养液振荡培养法较为准确可靠。 相似文献
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采用聚丙烯酰胺凝胶泳对来自太湖稻区的10个水稻稻瘟病菌株和2个禾本科杂草瘟菌的可溶性蛋白及酯酶同工酶进行了分析比较。结果表明:供试菌株菌体可溶性蛋白可呈现16~25条电泳谱带,其中在Rf值为0.23,0.33,0.44的3条谱带为122个菌株所共有,没有发现生理小种特异性谱带。对12个菌株α-酯酶同工酶的分析表明,在所有菌株同工酶谱带中可分辩出具有不同迁移率的12条谱带,据此可将菌株分为8个不同的同工酶谱带类型。同时表明,α-酯酶同工酶在太湖稻区稻瘟病菌中存在丰富的多态性,但其谱带类型与病菌生理小种无相关性。 相似文献
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苦楝内生真菌及其代谢产物的杀虫活性 总被引:2,自引:0,他引:2
在对290株苦楝内生真菌分离纯化鉴定的基础上,研究了30株具有代表性的内生真菌及其代谢产物的杀虫活性。结果表明其中5个菌株对马尾松毛虫5d校正死亡率大于60%,杀虫活性最强的KL042菌株经鉴定为球孢白僵菌。球孢白僵菌作为内生真菌,在国内尚未见报道。进一步选取杀虫活性较强的9株内生真菌,研究其代谢产物的胃毒和触杀活性,结果表明拟青霉KL017菌株发酵液10倍浓缩液对柳蓝叶甲胃毒活性最强,48h校正死亡率达90.09%。球孢白僵菌KL042、拟青霉KL017和交链孢KL062发酵液10倍浓缩液点滴马尾松毛虫前胸背板后,48h校正死亡率分别为76.67%、56.67%和53.33%。 相似文献
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近年来,广东省在应用白僵菌油剂行进飞机超低容量喷雾大面积防治马尾松毛虫方面,取得了较好的效果.而我省应用白僵菌高含孢量菌粉的油剂,进行飞机超低容量喷雾防治马尾松毛虫还是首次,特别是用于防治第一代马尾松毛虫,更是一次尝试。 相似文献
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稻瘟病菌生理小种酯酶同工酶特性的研究 总被引:4,自引:4,他引:4
运用聚丙烯酰胺凝胶等电聚焦法对来自江、浙两省经全国统一鉴别寄主鉴定的稻瘟病菌7群12个生理小种的16个菌株菌体酯酶同工酶进行了分析比较,结果表明:稻瘟菌菌体酯酶同工酶带有13-18条,等电点分布范围为3.00-7.20。在等电点4.15附近有各生理小种共同的酶带。同一生理小种群内不同生理小种间酯酶同工酶的相似系数一般大于0.50,不同生理小种群的生理小种间酶谱的相似系数一般为0.30-0.40,也存在少数不是同一生理小种群的小种间酶谱相似系数大于0.50的情况。供试各生理小种间酶谱均存在不同程度的差异,表现为酶带数目的多少和等电点分布的不同。来自不同省份经鉴别寄主鉴定为同一生理小种的菌株间酶谱的相似系数亦仅0.05-0.60。研究结果初步反映出供试大多数稻瘟菌菌株间菌体酯酶同工酶的相似性与致病性有关,但也受菌株来源地生态环境影响。本文还讨论了稻瘟病菌致病性容易变异但在许多情况下又具有相对稳定性的可能机制,探讨在一定范围内聚丙烯酰胺凝胶等电聚焦技术用于稻瘟菌生理分化和致病性变异监测的可能性及尚待进一步研究解决的穗题等。 相似文献
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中国小麦条锈菌生理小种同工酶分析 总被引:4,自引:1,他引:4
本文首次报道利用聚丙烯酰胺凝胶电泳技术分析我国小麦条锈菌11个主要流行生理小种及美国小麦条锈菌白化菌系夏孢子提取物中酯酶、过氧化物酶、过氧化氢酶、碱性磷酸酶、酸性磷酸酶、谷草酰胺转氨酶、多酚氧化酶的同工酶图谱。结果表明:我国小麦条锈菌不同生理小种具有同工酶表型异质性,而同一生理小种的不同单孢系间或不同地理来源的菌系间同工酶表型相同,即生理小种群体内部可能具有同工酶表型同质性。依据同工酶资料的统计分析结果,将所测的中国小麦条锈菌的11个生理小种划为5个类群:A群(条中8号)、B群(条中10号)、C群(条中17号)、D群(条中19、22、23、25、27、26号)、E群(条中28、29号),它反映出小种间遗传基础的相似性及演化特点。结合对美国条锈菌菌系同工酶测定,并与国外同类研究结果比较,揭示出中国小麦条锈菌具有独特的同工酶表型。同工酶表型分析是研究锈菌毒性变异的一个有力工具,建议建立起中国小麦条锈菌毒性菌系多种同工酶标准图谱,用作研究小麦条锈菌毒性变异和群体遗传学的参照系。 相似文献
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寄生沙棘木蠹蛾的白僵菌菌株特性及其胞外蛋白酶、几丁质酶的活性 总被引:1,自引:0,他引:1
[目的] 明确寄生沙棘木蠹蛾的白僵菌不同菌株的生物学特性,以筛选优良菌种加以利用。[方法] 测定温湿度对分离自沙棘木蠹蛾幼虫和蛹的5株白僵菌菌株分生孢子萌发的影响,并采用平板测定法和Lowry及DNS测定法对白僵菌各菌株胞外蛋白酶和几丁质酶活性进行测定。[结果] 白僵菌SJ 01、SJ 02、SJ 03、SJ 04、SJ 05菌株在15~35 ℃范围内均可萌发,15、20 ℃和35 ℃时孢子萌发率相对较低,25 ℃时5株菌株萌发率均高,为90%~98%。相对湿度为41%、50%和61%时,各菌株均不萌发;76%和80%时,SJ 05的萌发率明显高于其他菌株,分别达60%和80%。50 ℃高温胁迫24 min和30 min后,各菌株萌发率均显著降低,几乎不萌发。胁迫12 min和18 min后,SJ 05的萌发率较其他菌株高,表明其抗高温性强。5株菌株均可产生胞外蛋白酶和几丁质酶,其中SJ 05菌株胞外蛋白酶产量为55.3 U/mL,几丁质酶产量为137.9 U/mL,其蛋白酶和几丁质酶活性均显著高于其他菌株。[结论] SJ 05菌株具有较强的耐热性,一定的抗低湿能力,较高的蛋白酶和几丁质酶活性,为最优菌株。 相似文献
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The characteristics of 36 isolates of Leptosphaeria maculans originating from cv. Picra were investigated using cultural, pathogenicity and isozyme analyses in comparison with six known isolates of A- and B-groups. Picra-related isolates were similar to the three known B-group isolates from Brassica napus using a cultural test. Two known B-group isolates and 29 of the 36 Picra-related isolates tested were aggressive on cv. Picra, whereas all the three known A-group isolates were not. All the 42 isolates tested were aggressive on the B. napus – B. juncea recombinant line MX and on B. napus cv. Westar. Phosphoglucose isomerase analysis showed two different patterns, separating A-group known (fast band) from B-group known isolates (slow band). All cv. Picra isolates displayed the slow band. Considerable differences in isoesterase patterns were found between known A- and B-group isolates. Isolates recovered from cv. Picra displayed six different electrophoretic patterns, including that of the known B-group isolates. Clusters of the 42 isolates, generated by UPGMA analysis based on similarity coefficients of electrophoretic types, matched the previous classification into A- and B-groups, although two atypical Picra-related isolates with a singular pattern clustered with the A-group. The importance of these isolates for blackleg epidemiology and resistance breeding is discussed. 相似文献
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自然界分离到的黑胫茎点霉(Phoma lingan)常可发现有致病力和无致病力两类菌株,这两类菌株不但在生长速度和色素产生上有所不同,在几种同功酶图上也有不同。用淀粉胶法测定有致病力菌株和无致病力菌株的同功酶系统发现它们在7种酶系统中酶带位置和数目都表现不同.在MDH、IDH、AAT、GPT、PGM和EST的酶图中无致病力菌株移动较快,而在6PG的酶图中有致病力菌株移动较快,而在6PG的酶图中有致病力菌株移动较快.此外,无致病力菌株的MDHPGM、和AAT均有两条带,而有致病力菌株上只有一条带. 相似文献
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室内测定了从云南分离的7株球孢白僵菌对马铃薯块茎蛾幼虫的毒力,结果表明,在接菌孢子浓度为105个/mL和107个/mL下,接种后7d马铃薯块茎蛾幼虫累积死亡率分别为23.3%~70.0%和33.3%~86.7%,添加吐温80的清水对照死亡率仅为3.3%。筛选出毒力较高的Bb7001、Bb7004、Bb8001 3个菌株,其中菌株Bb8001对马铃薯块茎蛾幼虫的毒力最高,在孢子浓度1×105、1×107个/mL下,第7天的累计死亡率分别为70.0%和86.7%,其毒力回归方程Y=0.366X+3.409(R=0.956),剂量效应LC50=2.24×104个/mL,孢子浓度107个/mL下的LT50为4.3d。测定了毒力较高的菌株与12种农药的生物相容性,结果表明在供试农药推荐用量及其101、00倍稀释液下,球孢白僵菌Bb7001、Bb7004、Bb8001的3个菌株孢子在含所选农药的营养液中生物相容性良好的农药有:苏云金芽孢杆菌可湿性粉剂(Bt)、10%吡虫啉可湿性粉剂、2.5%菜喜悬浮剂及松毛虫病毒可湿粉剂,可配伍使用;而阿维菌素、农安、卡死克、抑太保对球孢白僵菌有很强的抑制作用,应避免配伍使用。 相似文献
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Twenty isolates of Puccinia graminis f.sp. tritici from South America were compared with 19 from Europe using virulence, isozymes and random amplified polymorphic DNA (RAPD) markers. The isozyme and virulence patterns for these isolates were also compared with those of 11 isolates representative of the common race clusters in North America. All three types of marker showed a level of similarity between the South American and European isolates comparable with that between isolates from the same continent. The average similarity coefficients between the South American and European isolates were 0.65 for virulence, 0.67 for isozymes, and 0.70 for RAPD markers. Among South American isolates the values were 0.63 for virulence, 0.64 for isozymes and 0.72 for RAPDs. For the South American and European isolates, correlation between the similarity matrices based on RAPDs and on isozyme markers, respectively ( r = 0.52), was higher than that between the RAPD and virulence matrices ( r = 0.32) or between isozyme and virulence matrices ( r = 0.16). The North American isolates had a comparable level of similarity for virulence and isozymes to both the South American and European populations. There was no clear distinction between the South American, North American and European isolates, which is consistent with the hypothesis that these populations may have had a common origin. 相似文献
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T. YLI-MATTILA S. PAAVANEN A. HANNUKKALA P. PARIKKA R. TAHVONEN & R. KARJALAINEN 《Plant pathology》1996,45(1):126-134
Differences in isozyme and RAPD-PCR polymorphisms amongst 33 isolates of Fusarium avenaceum were compared using native polyacrylamide gel electrophoresis and agarose gel electrophoresis. The isolates were collected from different regions of Finland. Amongst eight enzymes analysed clear isozyme polymorphism was detected in five enzymes which could be grouped into 20 different electrophoretic phenotypes and three main groups at the similarity level of 70% in unweighted pair group method with arithmetic average (UPGMA) analysis. RAPD-PCR analysis differentiated all F. avenaceum strains from each other. The phenotypes resulting from RAPD-PCR analysis were grouped into five main groups by UPGMA analysis at the similarity level of 55%. These main groups had several similarities with the main groups from isozyme analysis. RAPD-PCR patterns of 16 isolates of Fusarium graminearum F. culmorum F. equiseti F. oxysporum and F. redolens were also studied and strains from each Fusarium species formed individual groups in UPGMA and principal components analyses. Thus, the extent of isozyme and RAPD-PCR polymorphisms found in Fusarium strains potentially provides a method for identifying the fungi both at strain and species level. 相似文献