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1.
The objective of the present study was to evaluate the effect of the depth of insertion and environmental parameters on the rectal temperature (RT) in donkeys during the hot-dry season in a tropical savannah zone of Nigeria. The experimental subjects were comprised of thirty donkeys divided into three groups based on age: group I, 10 foals (40.67 ± 2.20 kg; 1.50 ± 0.02 months); group II, 10 yearlings (91.53 ± 0.54 kg; 1.51 ± 0.01 years); and group III, 10 adults (140 ± 0.71 kg; 8.03 ± 0.06 years). Each group was divided into 5 male and 5 female donkeys. Measurements of the RT were recorded with a digital thermometer probe (model HI935007, Hanna Instruments), which was inserted into the rectum at varying depths of 3.5, 7, 10.5, and 14 cm in the same animal in each group. There was a gradual increase in the RT in donkeys as the depth of insertion was increased from 3.5 cm (36.60°C) to 14 cm (38.40°C). Data obtained from the study were subjected to repeated-measures analysis of variance, followed by Tukey's post-hoc test to compare mean values between different depths of RT measurements. Overall, there was a variation in the RT by the depth of insertion with the shallow depth of 3.5 cm having a lower RT than the depths of 7, 10.5, and 14 cm. The variation of the RT observed in donkeys showed that there is need to standardize the probe-insertion depth in veterinary clinical practice for accurate measurement of the RT in donkeys in the Northern Guinea savannah zone of Nigeria.  相似文献   

2.
Coat colour contributes to physiological adaptation in mammals and mediates response to thermal stress. Twenty-four adult West African Dwarf sheep of both sexes and with different coat colour types were used in this study. We measured rectal temperature (RT), respiratory rate (RR) and pulse rate (PR) before sunrise and sunset during the late dry season (January–March) and early rainy season (April–June) as well as packed cell volume (PCV), red blood cell (RBC) count, white blood cell (WBC) count, plasma sodium (Na+) and potassium (K+). Animals with black coat colour had the highest (P?<?0.05) mean values of 38.92?±?0.03 °C, 65.09?±?1.06 breaths/min, 81.35?±?0.78 beats/min, 1.70?±?0.01 for RT, RR, PR and heat stress index (HSI), respectively, followed by brown mouflon and brown with extensive white, while the Badger Face coloured sheep had the least mean values. There were significant (P?<?0.05) differences between male and female sheep for RT, RR, PR and HSI. Season had a significant (P?<?0.05) effect on RT, RR, PR and HSI. Coat colour and sex also significantly (P?<?0.01) affected RBC, WBC, Na+ and K+. Seasonal variation (P?<?0.05) in all the blood parameters was observed, with the exception of PCV. Interaction effect of coat colour and sex was significant (P?<?0.05) on RT and HSI. Correlation coefficients among the measured traits ranged from positive to negative values. These results indicate that selection of white-coloured sheep to attenuate heat stress is desirable in the hot humid tropics.  相似文献   

3.
Genetic analyses in donkeys are likely to face compromises in terms of sample size and population structure. This study aims at implementing a suitable model to estimate breeding values and genetic parameters for gaits in Andalusian donkeys. Empirical observation revealed that ambling donkeys (showing a slightly uneven, non-isochronous 1–2, 3–4 lateral sequence gait) did not walk (i.e. presented an isochronous, even 1-2-3-4 sequence gait) and vice versa. However, the two donkey groups could trot, equally. In this study, 2700 gait records were registered from 300 donkeys. The sample included 1350 gait records from 169 ambling/trotting donkeys and 1350 gait records from 131 walking/trotting donkeys. Fixed effects included year, season, sex, farm/owner, husbandry system, weather, ground type and appraisers. Weight and age were included as covariates. MTDFREML software was used to estimate (co)variance components, genetic parameters and predict breeding values and their accuracies in both sets, separately. Gaits’ heritability?±?SE estimates were 0.56?±?0.155, 0.53?±?0.317 and 0.67?±?0.166 for amble, walk and trot, respectively. Genetic correlations were 0.31?±?0.216, 0.42?±?0.115 and 0.28?±?0.178, for amble and walk, amble and trot and walk and trot, respectively. Not all gaits are suitable to treat every human sensomotor condition. We developed a locomotion selection index, assessing the relative loss/gain in index accuracy when each gait modality was excluded to develop different gait specific therapeutic lines to genetically select the best performing donkeys from each gait modality. Our results suggest that gait genetic lines could be developed and may be potential selection criteria to consider in assisted-therapy donkey breeding programs.  相似文献   

4.
The present study was formulated to find out the status of important season related thermal stress biomarkers of pure-bred (Hampshire) and crossbred (50% Hampshire × 50% local) pigs under the agro-climatic condition of Assam State, India. The experiment was also aimed to study the role of different level of energy ration (110, 100, and 90% energy of NRC feeding standard for pig) in variation of physiological and biochemical parameters in two genetic groups of pigs in different seasons. The metabolizable energy value were 3260, 2936.5, and 3585.8 kcal/kg in grower ration and 3260.2, 2936.6, and 3587 kcal/kg in finisher ration for normal energy (NE), low energy (LE) and high energy (HE), respectively. Both the genetic group of animals were housed separately under intensive system of management. Each pen was measuring 10′ × 12′ along with an outer enclosure. Six weaned piglets (almost similar body weight of average 10.55 kg) of each group were kept in a separate pen. However, after attainment of 35 kg body weight, the animals of a group were divided in two pens of three animals each. The present experiment indicated that average ambient temperature during summer months (27.33–29.51 °C) was above the comfort zone for pigs (22 °C). The significantly (P?<?0.01) higher relative humidity (RH) (%) was recorded in outdoor environment (87.26–91.10%) and in the morning time (86.60–91.10%). The temperature humidity index (THI) during the study period was found to be indicative of thermal stress to the experimental animals during summer (79.55–82.56). Physiological parameters viz., respiration rate (RR) and rectal temperature (RT) were significantly (P?<?0.01) higher in summer season (43.75–72.12 breaths/min. and 102.29–103.23 °F) and non-significantly higher values were recorded in Hampshire pigs. It was also found that the significantly (P?<?0.01) lower RR as well as RT was recorded in the pigs fed with high energy (HE) ration during summer season. Serum triiodothyronine (T3) and thyroxine (T4) concentrations were significantly (P?<?0.01) lower during summer, while both the genetic groups showed significantly (P?<?0.01) higher concentration of serum cortisol during summer season. It was also observed that thyroid hormone and cortisol concentrations were maintained in groups of pig fed vegetable oil incorporated HE diet during summer. From the present study, it is found that the increasing the energy level of the ration might be helpful to minimize the effects of thermal stress during summer.  相似文献   

5.

Objective

To compare the induction and recovery characteristics and selected cardiopulmonary variables of midazolam–alfaxalone or midazolam–ketamine in donkeys sedated with xylazine.

Study design

Randomized, blinded, crossover experimental trial.

Animals

A group of seven adult male castrated donkeys weighing 164 ± 14 kg.

Methods

Donkeys were randomly administered midazolam (0.05 mg kg?1) and alfaxalone (1 mg kg?1) or midazolam (0.05 mg kg?1) and ketamine (2.2 mg kg?1) intravenously following sedation with xylazine, with ≥ 7 days between treatments. Donkeys were not endotracheally intubated and breathed room air. Time to lateral recumbency, first movement, sternal recumbency and standing were recorded. Induction and recovery were assigned scores between 1 (very poor) and 5 (excellent). Heart rate (HR), respiratory rate (fR), invasive arterial blood pressures and arterial blood gases were measured before induction and every 5 minutes following induction until first movement.

Results

Time to lateral recumbency (mean ± standard deviation) was shorter after alfaxalone (29 ± 10 seconds) compared with ketamine (51 ± 9 seconds; p = 0.01). Time to first movement was the same between treatments (27 versus 23 minutes). Time to standing was longer with alfaxalone (58 ± 15 minutes) compared with ketamine (33 ± 8 minutes; p = 0.01). Recovery score [median (range)] was of lower quality with alfaxalone [3 (2–5)] compared with ketamine [5 (3–5); p = 0.03]. There were no differences in HR, fR or arterial pressures between treatments. No clinically important differences in blood gases were identified between treatments. Five of seven donkeys administered alfaxalone became hypoxemic (PaO2 <60 mmHg; 8.0 kPa) and all donkeys administered ketamine became hypoxemic (p = 0.13).

Conclusions and clinical relevance

Both midazolam–alfaxalone and midazolam–ketamine produced acceptable anesthetic induction and recovery in donkeys after xylazine sedation. Hypoxemia occurred with both treatments.  相似文献   

6.
Experiments were performed to investigate the effect of ascorbic acid (AA) in reducing hemato-biochemical changes in pack donkeys during the cold-dry (harmattan) season. Six experimental donkeys administered orally AA (200 mg/kg) and six control donkeys not administered ascorbic acid were subjected to packing. Blood samples were collected from all donkeys for hematological and biochemical analyses. In the control donkeys, packed cell volume (PCV), erythrocyte count and hemoglobin concentration (Hb) decreased significantly (P<0.05) at the end of packing. In the experimental donkeys, there was no significant difference between the pre- and post-packing values of PCV, erythrocyte count and Hb. In the control donkeys, the neutrophil and neutrophil:lymphocyte ratio increased significantly (P<0.05) post packing, but in the experimental donkeys, the pre- and post-packing values were not significantly different. The eosinophil count increased significantly (P<0.05) in experimental and control donkeys post packing. In conclusion, packing exerted significant adverse effects on the hematological parameters ameliorated by AA administration. AA may modulate neutrophilia and induce a considerable alteration of erythroid markers in donkeys subjected to packing during the harmattan season.  相似文献   

7.
The objective of this study was to investigate the use of infrared thermography (IRT) for detection of foot lesions in dairy cattle. Thermal images of the rear feet of 139 lactating dairy cows were taken with a thermal camera and evaluated using imaging software. Foot lesions identified in the study included white line disease (WLD), sole ulcer (SU) and digital dermatitis (DD). Temperatures at the coronary band (CBT) and the skin (ST) were recorded. Cows were scored for locomotion on a scale of 1–5 (1?=?normal and 5?=?severely lame). CBT was higher for all types of foot lesion (34.1?±?2.3, 33.8?±?1.6 and 33.1?±?1.6°C for WLD, SU and DD, respectively) than for healthy (32.6?±?1.9°C) feet. ST was higher only for WLD (33.2?±?2.0°C) compared to healthy feet (31.5?±?1.7°C). ΔT (temperature difference between CBT and ST) was higher for SU (2.1?±?0.8°C) than healthy feet (1.1?±?0.9°C). Locomotion scores (LSs) were similar between healthy cows (2.2?±?0.7) and DD (2.4?±?0.7) but greater for SU and WLD cows (3.0?±?0.9, 3.0?±?0.8) than healthy cows. The threshold values established for CBT and ST were 33.5°C (sensitivity?=?77.8%, specificity?=?65.2%) and 33.7°C (sensitivity?=?44.4%, specificity?=?92.9%) for SU, and 34.4oC (sensitivity?=?28.1%, specificity?=?88.6%) and 31.8°C (sensitivity?=?60.7%, specificity?=?55.4%) for overall lesion, respectively. These results indicate that IRT can be a useful tool for detection of SU, but not WLD and DD. In addition, CBT and ΔT were the best indicators for the detection of foot lesions.  相似文献   

8.
Disposition following single intravenous injection (2 mg/kg) and pharmacodynamics of cefquinome were investigated in buffalo calves 6–8 months of age. Drug levels in plasma were estimated by high-performance liquid chromatography. The plasma concentration–time profile following intravenous administration was best described by a two-compartment open model. Rapid distribution of cefquinome was evident from the short distribution half-life (t ½α ?=?0.36?±?0.01 h), and small apparent volume of distribution (Vdarea?=?0.31?±?0.008 L/kg) indicated limited drug distribution in buffalo calves. The values of area under plasma concentration–time curve, elimination half-life (t ½β ), total body clearance (ClB), and mean residence time were 32.9?±?0.56 μg·h/mL, 3.56?±?0.05 h, 60.9?±?1.09 mL/h/kg, and 4.24?±?0.09 h, respectively. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration of cefquinome were 0.035–0.07 and 0.05–0.09 μg/mL, respectively. A single intravenous injection of 2 mg/kg may be effective to maintain the MIC up to 12 h in buffalo calves against the pathogens for which cefquinome is indicated.  相似文献   

9.

Objective

To evaluate the onset, magnitude and duration of thermal antinociception after oral administration of two doses of tapentadol in cats.

Study design

Prospective, randomized, blinded, experimental study.

Animals

Six healthy adult cats weighing 4.4 ± 0.4 kg.

Methods

Skin temperature (ST) and thermal threshold (TT) were evaluated using a wireless TT device up to 12 hours after treatment. Treatments included placebo (PBO, 50 mg dextrose anhydrase orally), buprenorphine (BUP, 0.02 mg kg?1) administered intramuscularly, low-dose tapentadol (LowTAP, 25 mg orally; mean 5.7 mg kg?1) and high-dose tapentadol (HighTAP, 50 mg orally; mean 11.4 mg kg?1) in a blinded crossover design with 7 day intervals. Statistical analysis was performed using anova with appropriate post hoc test (p ≤ 0.05).

Results

Salivation was observed immediately following 11 out of 12 treatments with tapentadol. The ST was significantly increased at various time points in the opioid treatments. Hyperthermia (≥ 39.5 °C) was not observed. Baseline TT was 45.4 ± 1.4 °C for all treatments. Maximum TT values were 48.8 ± 4.8 °C at 1 hour in LowTAP, 48.5 ± 3.0 °C at 2 hours in HighTAP and 50.2 ± 5.3 °C at 1 hour in BUP. TT significantly increased after LowTAP at 1 hour, after HighTAP at 1–2 hours, and after BUP at 1–2 hours compared with baseline values. TTs were significantly increased in BUP at 1–2 hours compared with PBO.

Conclusion and clinical relevance

Oral administration of tapentadol increased ST and TT in cats. The durations of thermal antinociception were similar between HighTAP and BUP, both of which were twice as long as that in LowTAP. Studies of different formulations may be necessary before tapentadol can be accepted into feline practice.  相似文献   

10.
During summer season, increase in the environmental temperature in the subtropical regions of Pakistan is negatively affecting the performance of dairy animals. The study objective was to determine the effect of ambient management (90 days) on productive and physiological performance of lactating Sahiwal cows during hot dry summer season. Fifteen lactating cows during the early lactation stage, having similar parity (3), daily milk production (6.2 l), were randomly allocated to three treatments, 5 cows each, i.e., (1) kept under roof shade only, (2) provision of fans along with roof shade, and (3) provision of roof shade, fans, and sprinklers designated as S, SF, and SFS, respectively. The fans were of 360-rpm capacity and showers were on for 40 min after every 90-min interval from 9:00 to 21:00 h. THI values were 81.1?±?0.7, 80.5?±?0.7, and 77.7?±?0.4 under S, SF, and SFS treatments, respectively. Cows were milked twice daily. Respiration rate (RR) and rectal temperature (RT) data were collected at 14:00 h on daily basis. The daily milk production was significantly higher in cows under SF (7.9?±?1 kg) followed by SFS (6.9?±?1.2 kg) and S (6.1?±?0.9 kg) treatments. The mean RT (101.0?±?0.04 °F) was significantly lower in cows under SFS than that on SF and S treatments and similarly mean RR was also lower (21.2 breaths/min) in cows under SFS followed by SF and S treatments. It is concluded that milk production and physiological performance in Sahiwal cows can be improved by fan-assisted ventilation during hot dry summer in subtropical regions.  相似文献   

11.
A serial ultrasonographic study was conducted on nine jennies aged 5–15 years from January to April 2008 with the objective of studying ovarian follicular dynamics and estrus manifestations under controlled management. Ovarian follicular activity was determined from the number and size distribution of follicles, length of interovulatory interval (IOI), growth rate of preovulatory follicles, diameter of follicles at the onset of estrus, and incidence of ovulation. Estrus manifestations were characterized using length of estrus and estrous cycle. The mean (±SD) number of follicle detected per ovary was 5.45?±?2.3 (range, 1–16) with sizes ranging from 2.9 to 44 mm. The mean (±SD) size of follicle encountered at the onset of estrus was 25.9?±?3.7 mm (range, 20.9–34.4) while that of the preovulatory follicles at ?1 day before ovulation was 36.81?±?3.78 mm. The mean (±SD) IOI, estrus, and estrous cycle length were 25.4?±?3.6, 7.9?±?2.9, and 24.2?±?7.4 days, respectively. The mean (±SD) growth rate of the preovulatory follicle after the day of divergence was 1.9?±?0.3 mm/day. Serum progesterone profile followed the same patterns of ovarian dynamics with maximum values being detected during midluteal phase. Serum progesterone assay revealed blood progesterone profiles of <1.0 ng/ml during estrus and up to 11 ng/ml during midluteal phase with a pattern following follicular dynamics. Body condition of the study jennies steadily increased and was positively correlated (r?=?0.52, p?<?0.001) with the diameter of the preovulatory follicle. In conclusion, the ultrasonic evaluation has revealed that follicular dynamics of jennies were generally related with body condition which might have been influenced by the type of management.  相似文献   

12.
The Girolando breed is used in pasture-based dairy production systems in Brazil to associate the high production of Bos taurus to the rusticity and thermal adaptation of Bos indicus. This study was designed to evaluate the physiological response to a gonadotropin-releasing hormone (GnRH)–prostaglandin F (PGF)–GnRH protocol to synchronize the ovulation in 40 Girolando heifers of a pasture-based dairy production system and its relationships with the temperature and humidity index (THI) during the dry (DS) and rainy season (RS) in the tropical savannah—Brazil's cerrado biome. Responses were characterized by follicular and corpus luteum number and diameter, ovulation (D9), and pregnancy rates after first AI. Total follicle number (8.1?±?0.3?×?8.8?±?0.3), D9 ovulatory follicle diameter (11.9?±?0.4?×?10.1?±?0.4 mm), corpus luteum diameter (8.6?±?1.3?×?3.9?±?1.5 mm), corpus luteum score (3.7?±?0.8?×?1.8?±?1.0), corpus luteum diameter after AI (9.6?±?1.6?×?3.9?±?1.5 mm), and corpus luteum score after AI (3.2?±?0.4?×?0.9?±?0.6) in DS and RS differed (P?<?0.01). D9 ovulation rate was 40 % (DS) and 20 % (RS), without differences (P?>?0.05). Pregnancy rate was 45 % (DS) and 11 % (RS), with differences (P?<?0.01). THI differed between DS and RS (P?<?0.01). THI may interfere in the follicular and luteal dynamics and in the response of Girolando heifers to the GnRH–PGF protocol in the tropical savannah, thus reducing the chances of pregnancy at the first artificial insemination.  相似文献   

13.
The study was conducted to assess early-expressed reproductive traits of Boran cattle and their crosses with Jersey and Holstein Friesian (HF). The traits studied were age at first services (AFS), number of services for first conception, age at first calving (AFC), first dry period (FDP), first calving interval (FCI), and first service period (FSP). Genetic group and period of birth/calving had a significant (p?<?0.05) effect on reproductive traits. The Boran cattle were inferior to HF or Jersey crosses. First crosses (F 1) for Jersey and Boran (50 % Jersey: 50 % Boran) showed a significantly (p?<?0.05) younger AFS (by 7.25 months) and AFC (by 10.75 months), had shorter FCI (by 63.27 days), FDP (by 61.13 days), and FSP (by 60.3 days), and needed less (by 0.35) numbers of services per first conception as compared to the Boran cattle. The F 1 for Jersey and Boran (50 % Jersey: 50 % Boran) crosses showed better performance than the F 1 for HF and Boran (50 % HF: 50 % Boran). Heritability values for AFS and AFC were the highest and were estimated at 0.51?±?0.10 and 0.49?±?0.13, respectively, and lowest heritability was recorded for FDP (0.02?±?020) and FSP (0.10?±?0.29). The genetic correlation was highest (0.10?±?0.20) between AFS and AFC and was lowest (?0.01?±?0.66) between FCI and FSP. The breed additive for Jersey was only significant (p?<?0.01) for AFS and AFC. The crossing of HF with Boran cattle has desirably reduced 9.16?±?2.88 months in AFS; the corresponding reduction in AFS was 3.49?±?3.59 months by crossing with Jersey. The performance comparisons and genetic and crossbreeding parameters indicated that crossbreeding of Boran with HF or Jersey can improve reproductive performance.  相似文献   

14.
ObjectiveTo compare a towel under, a warm water pad under or a forced warm air blanket over dogs as techniques to reduce heat loss during a standardized anesthetic.Study designProspective, randomized, crossover study.AnimalsEight, healthy, mixed breed dogs weighing 16.3–19.6 kg.MethodsDogs were anesthetized four times for 90 minutes. Dogs were placed on a steel table (treatment TA), with a cotton towel (treatment TO) or a circulating warm water pad (treatment WP) between the dog and the table, or with, a towel under the dog and covered with a forced warm air blanket (treatment WAB). Rectal temperature (RT) was recorded at 5 minute intervals. Changes in temperature (ΔRT) were calculated as the RT at a given point subtracted from the RT before anesthesia (baseline) and compared over time.ResultsAfter 90 minutes of anesthesia, the ΔRT was 3.42 °C ± 0.29 for TA, 2.78 °C ± 0.43 for TO, 1.98 °C ± 0.29 for WP, and 0.91 °C ± 0.27 for WAB. Significant differences in ΔRT occurred between TA and WAB at 20 minutes (0.94 °C ± 0.42, p = 0.0206), between TO and WAB at 30 minutes (1.16 °C ± 0.62, p = 0.0063), between WP and WAB at 50 minutes (0.96 °C ± 0.98, p = 0.0249), between TA and WP at 35 minutes (1.19 °C ± 0.54, p = 0.0091), between TO and WP at 70 minutes (1.12 °C ± 0.56, p = 0.0248), and between TA and TO at 75 minutes (0.96 °C ± 0.62, p = 0.0313). These differences in ΔRT between each treatment persisted from the times indicated until the end of the anesthesia.Conclusion and clinical relevanceDuring anesthesia, forced warm air blankets were superior to other methods tested for limiting heat loss. An efficient heat loss technique should be used for anesthesia longer than 20 minutes duration in medium sized dogs.  相似文献   

15.

Objectives

To validate a thermal threshold (TT) nociceptive model in bearded dragons (Pogona vitticeps) and to document TT changes after administration of morphine.

Study design

A two-part randomized, blinded, controlled, experimental study.

Animals

Five adult bearded dragons (242–396 g).

Methods

A TT device delivered a ramped nociceptive stimulus (0.6 °C second?1) to the medial thigh until a response (leg kick/escape behavior) was observed or maximum (cut-off) temperature of 62 °C was reached. In phase I, period 1, six TT readings were determined at 20 minute intervals for evaluation of repeatability. Two of these readings were randomly assigned to be sham to assess specificity of the behavioral response. The same experiment was repeated 2 weeks later (period 2) to test reproducibility. In phase II, animals were administered either intramuscular morphine (10 mg kg?1) or saline 0.9%. TTs (maximum 68 °C) were determined before and 2, 4, 8, 12 and 24 hours after treatment administration. Data were analyzed using one-way anova (temporal changes and repeatability) and paired t tests (reproducibility and treatment comparisons) using Bonferroni correction (p < 0.05).

Results

Mean TT values were 57.4 ± 3.8 °C and 57.3 ± 4.3 °C for periods 1 and 2, respectively. Data were repeatable within each period (p = 0.83 and p = 0.07, respectively). Reproducibility between periods was remarkable (p = 0.86). False-positive responses during sham testing were 10%. TTs were significantly increased after morphine administration at 2, 4 and 8 hours compared with baseline, and at 2 and 4 hours compared with saline 0.9%. The highest TT was 67.7 ± 0.7 °C at 4 hours after morphine administration.

Conclusions and clinical relevance

Testing was repeatable, reproducible and well tolerated in bearded dragons. TT nociceptive testing detected morphine administration and may be suitable for studying opioid regimens in bearded dragons.  相似文献   

16.
This work was designed to evaluate the ovarian follicular development, oocytes morphology, methods of oocytes reterival, and the effect of different in vitro maturation (IVM) media on cumulus cell expansion and nuclear maturation of Jennies oocytes. Experiment 1, the number of small (<6 mm), medium (6 to 9 mm) and large size (>10 mm) ovarian follicles was recorded. Cumulus-oocyte-complexes (COCs) were reterived and classified into 4 Grades based on their cumulus-cells investment and the homogenous of the ooplasm. In Experiment 2, COCs were recovered by using 18-G, 20-G needle or slicing and scraping of ovarian follicles to determine the number and morphology of the recovered COCs. In Experiment 3, Grade A and B COCs were IVM in DMEM-HG, DMEM-LG, DMEM-F12, TCM199, TCM199-F12 or CR1aa media supplemented with 10 % FCS?+?10 μg FSH/mL?+?10 IU hCG/mL?+?50 μg/mL gentamicin. Maturation was performed for 36 h at 38.5 °C under 5 % CO2 in humidified air. After IVM, cumulus cell expansion and oocytes nuclear canfiguration were determined. An average of 6.40?±?0.26 follicles was recorded per Jenny ovary, representing 3.37?±?0.46, 1.89?±?0.14 and 1.14?±?0.16, for the small, medium and large size follicles, respectively. Oocyte recovery was higher (P?P?P?P?P?P?P?P?Conclusion: Slicing and scraping or aspiration of follicles using 18-G needle increased the number and percentage of Grade A Jennies oocytes. TCM199-F12, CR1aa and TCM199 medi are more suitable for IVM of Jenny oocytes by promoting cumulus cells expansion and nuclear maturation to M II stage.  相似文献   

17.
Six Nubian pack donkeys were used to determine variations in rectal temperature (RT), respiratory rate (RR), and heart rate (HR) during the early rainy season. The donkeys exhibited a diurnal range of RT, RR, and HR of 34.7 to 38.7°C, 15 to 75 breaths/minute, and 36 to 72 beats/minute, respectively, with peak mean values at 2:00 pm and a trough shortly after sunrise (7:00 am). The amplitude of the diurnal rhythm of RT and RR was not correlated with the diurnal range of ambient temperature (AT) and relative humidity (RH). Although mean diurnal RTs were positively correlated with corresponding ATs, mean daily RTs were not significantly correlated with corresponding ATs. Diurnal and daily variations in RT of donkeys, like those of other ungulates, therefore, appear to reflect an endogenous rhythm, which may be largely independent of fluctuations in environmental thermal load. The mean maximum AT and RH, and the corresponding RR and HR values, fell above the normal range for donkeys in the tropics. The season was meteorologically stressful to the donkeys, imposing significant changes in the RT, RR, and HR parameters requiring responses to maintain homeostasis. The values of the amplitude in RT, RR, and HR obtained in individual donkeys were higher than the corresponding values obtained during the hourly recordings, indicating that it is more important to evaluate the individual's vital parameters in reaching conclusion of fitness for work in donkeys. The hourly recordings have shown the hours of the day (11:00 am to 4:00 pm) that are potentially meteorologically stressful for donkeys to be used for packing during the season.  相似文献   

18.
Lincomycin 10 mg kg?1, IV in buffalo calves followed two-compartment open model with high distribution rate constant α (11.2?±?0.42 h?1) and K 12/K 21 ratio (4.40?±?0.10). Distribution half-life was 0.06?±?0.01 h and AUC was 41.6?±?1.73 μg mL?1 h. Large Vdarea (1.15?±?0.03 L kg?1) indicated good distribution of lincomycin in various body fluids and tissues. Peak plasma level of lincomycin (71.8?±?1.83 μg mL?1) was observed at 1 min as expected by IV route. The elimination half-life and MRT of lincomycin were short (3.30?±?0.08 and 4.32?±?0.11 h, respectively). Lincomycin 10 mg kg?1 IV at 12-h interval would be sufficient to maintain T?>?MIC above 60 % for bacteria with minimum inhibitory concentrations (MIC) values ≤1.6 μg mL?1. Favourable pharmacokinetic profile in buffalo calves and a convenient dosing interval suggest that lincomycin may be an appropriate antibacterial in buffalo species for gram-positive and anaerobic bacterial pathogens susceptible to lincomycin.  相似文献   

19.
This study aimed to investigate the influence of growth rate and onset of boar contact on age at first observed estrus of the replacement gilts raised in Thailand. In total, 766 gilts were measured for body weight and backfat thickness prior to insemination. Body weight was further calculated for growth rate. Estrus detection was performed twice a day by back pressure test with an existence of mature boars with high libido. The first date of boar exposure and that of first observed estrus were individually recorded. Due to growth rate, they were classified into three groups: high (>700 g/day), moderate (600–700 g/day), and low (<600 g/day). According to onset of boar contact, the gilts were grouped into two categories: early (<150 days) and late (≥150 days). The results revealed that the gilts expressed first observed estrus, averagely, at age 205.1?±?34.1 days, had a growth rate of 615.5?±?57.6 g/day, and first contact with boars at 160.7?±?19.9 days of age. The gilts with low growth rate expressed first estrus later than those with moderate (208.6?±?2.0 vs 198.0?±?3.2 days, P?=?0.033) and high growth rate (208.6?±?2.0 vs 193.9?±?6.7 days, P?=?0.005) groups. Together with the influence of boar exposure, the gilts contacted boar earlier with high growth rate showed first estrus at age 180.3?±?10.1 days, whereas those with later boar contact with low growth rate showed first estrus at age 197.9?±?3.2 days. In summary, the replacement gilts should have high growth rate and contact boar early to attain puberty faster and possess decent subsequent reproductive performance.  相似文献   

20.
The objective of present study was to evaluate the growth potential of Sahiwal calves for veal production on whole milk or whole milk and milk replacer combined in a ratio of 50:50 (MMR). For this purpose, 48 Sahiwal calves (both male and female) were assigned to four dietary treatments having 12 animals/treatment. Calves in the treatments A and B were offered whole milk at 15 or 20 % of their body weight (BW), respectively, up to day 84 adjusted on weekly basis. The calves in treatments C and D received the same amount of milk as in treatments A and B until day 21, respectively, after which 50 % of the milk offered was replaced with a blend of chickpea (Cicer arietinum) flour and vegetable (corn) oil mixed in water (MR) until day 84. The constituted MR had 3.1, 2.8, and 14.3 %, CP, EE, and DM, respectively. The growth and intake data were analyzed using repeated measures analysis, with MIXED Procedures of SAS in a 2?×?2 factorial design. The two factors were feeding level and feeding source. Calves offered whole milk grew faster (P?<?0.05) and had greater weaning weights (P?<?0.05) than those offered MMR (606.4?±?18.1 vs 331.3?±?18.1 g/day and 70.4?±?1.5 vs 47.8?±?1.5 kg, respectively). Greatest daily BW gain (656?±?26 g/day) and weaning weight (74.6?±?2.1 kg) were observed in calves given treatment B while the lowest production cost/kg of BW gain (≈US$3.6?±?0.2) was observed in calves given treatment A. The daily BW gain of calves fed milk ad libitum was 716?±?40 and 836?±?40 g/day, during 5–8 and 9–12 weeks, respectively. The number of days calves exhibited scours was higher in calves offered MMR than those offered whole milk. Replacement of 50 % milk with a blend of chickpea flour and vegetable oil, as an alternative to milk replacer, did not support growth equivalent to whole milk and was not effective in reducing feeding cost during the weaning period. Sahiwal calves may have promise for being raised for veal production under tropical environments.  相似文献   

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