放牧和封育条件下科尔沁沙地小叶锦鸡儿群落土壤种子库特征

以科尔沁沙地小叶锦鸡儿群落为研究对象,对比分析了放牧干扰和不同封育年限下土壤种子库特征。结果表明:①放牧和封育样地土壤种子库均以一年生植物为主,物种丰富度没有明显差异;②放牧样地土壤种子库密度为(3 475±519)粒/m2,封育6年和封育12年样地土壤种子库密度分别为(20 241±1 714)粒/m2和(28 777±3 946)粒/m2,显著高于放牧样地;③Shannon-W iener多样性指数、S impson多样性指数和P ielou均匀度指数在放牧样地分别为1.36,0.68和0.56,并随着封育年限的增加而降低;④放牧和封育样地土壤种子库主要分布于0~2 cm的土壤表层,封育样地各层的种子库密度均显著高于放牧样地;⑤放牧和封育样地土壤种子库均为聚集分布,但放牧样地种子库的空间异质性高于封育样地。     

塔里木河上游湿地土壤种子库特征

以塔里木河上游阿拉尔地区不同湿地类型土壤种子库为研究对象。通过野外调查取样和室内萌发试验相结合的方法,对土壤种子库的组成、物种多样性和空间分布特征进行分析。研究结果表明:研究区内各湿地类型土壤种子库物种匮乏,多样性低,萌发的物种数在仅10-13种之间,种子库密度在168-2093粒/m2之间,属于较低水平。各湿地土壤种子库密度表现为人工湿地>河流湿地>湖泊湿地,受保护的人工湿地土壤种子库最高。种子库的空间分布为聚集分布。     

子午岭主要森林类型土壤种子库研究

对子午岭天然油松林、人工油松林、人工落叶松林、人工刺槐林四种林分的土壤种子库特征初步研究结果表明 :人工油松林种子库储量平均为 675 .9粒 /m2 ,人工刺槐林为648.8粒 /m2 ,人工落叶松林为 839.4粒 /m2 ,天然油松林为 45 5粒 /m2 ,各林分土壤种子库储量属中等水平 ;本次调查的天然油松林种子库种子活力为零 ,人工油松林种子库活力种子占 0 .5 % ,人工落叶松林种子库活力种子占 4.2 % ,刺槐林种子库活力种子占 74.4%。除人工刺槐林种子库种子质量较高外 ,针叶林各林分种子库种子质量都较差 ;人工油松林林下油松幼苗的分布密度平均为 1～ 3株 /m2 ,人工刺槐林林下多见灌木幼苗 ,另两种林分林下幼苗较少 ,表明不同林分土壤种子库种子都具有一定的萌发能力 ;从各林分土壤种子库的组成看 ,主要树种的种子在种子库中所占比例都在 85 %以上 ,人工油松林土壤种子库物种多样性指数较高 ,而其它林分土壤种子库物种多样性指数均较小 ;土壤种子库中 80 %左右的种子集中分布在枯枝落叶层和 0 - 2 cm土壤层中 ,下层种子数量很少 ;方差分析表明 ,枯枝落叶层厚度、树高、郁闭度、胸径等因子变化对土壤种子库储量影响显著。综合分析认为 ,子午岭主要森林类型土壤种子库能够为林分的天然更新提供基本条件。     

塔里木河下游植被退化区土壤种子库特征

本文在塔里木河下游选择了四个典型荒漠植被退化区,通过土壤种子库萌发实验,对土壤种子库的基本特征以及与地上植被的关系进行了对比研究。结果表明:1)四个植被退化区土壤种子库总共含有10个物种(属),平均密度1633.33粒/m2。2)四个植被退化区种子库密度总和来看,0-5cm层>5-10cm>层10-30cm层,但在各个研究区中种子库密度的垂直分布异质性差异很大,没有一致的规律。3)各个研究区土壤种子库种子的生活型组成,草本物种密度均明显高于灌木。4)土壤种子库与地上植被的相似性系数在四个研究区均很低;随着退化程度的加重,种子库的密度和物种数均呈现明显的下降趋势,但土壤种子库物种组成的稳定性要高于地上植被。     

毛乌素沙地天然臭柏群落种子产量、种子库及幼苗更新

采用样方调查法,研究了毛乌素沙地臭柏(Sabinavulgaris)群落的种子产量、种子库及幼苗更新。结果表明:毛乌素沙地天然臭柏群落的球果丰富,约为3093. 83粒/m2,种子产量为9118. 5粒/m2,优良种子达2954. 4粒/m2。5种立地条件间的土壤种子库差异大、质量低,种子优良度最高的也仅为3. 7%。土壤种子集中分布于0 ~5cm土层内,并随着土壤深度增加,优良种子数由0~5cm的30. 52粒/m2减少到5~10cm的1. 3粒/m2。5 种立地中当年幼苗数的平均值仅为7. 98株/m2,最高的(A类立地)为14. 7株/m2,仅占表土优良种的30%左右,最低的(D类立地)已经丧失了更新能力。臭柏群落内种子更新苗少,而且种子更新集中在少数滩地小生境中。种子库小、质量差、更新环境的丧失是限制臭柏群落天然更新与拓展的主要因子。     

黄土丘陵沟壑区植被自然恢复过程中土壤种子库特征

采用野外调查取样和室内实验相结合的方法,研究了黄土丘陵沟壑区植被自然恢复过程中土壤种子库的物种组成、密度、物种多样性以及相似性特征.结果表明:阴、阳坡10个样地中,共萌发了55种2203株幼苗,隶属于23科48属;总密度达到5 505±625.537粒/m2,且0～5 cm土层远远大于5～10 cm土层的土壤种子库密度,约是其4.6倍;丰富度、多样性、均匀度指数均未与恢复年限达到显著相关水平,但生态优势度随恢复年限的增加呈上升趋势,且阳坡上升的趋势比阴坡明显;组成成分相似性系数变化范围为0.0952～0.7143,恢复年限相距越大,其相似性越小,说明植被恢复演替的过程也是土壤种子库空间异质性加大的过程.     

沙漠化过程土壤种子库特征的研究

采用野外定点取样与室内萌发实验相结合的方法 ,研究了 4种不同类型退化沙地土壤种子库特征 ,主要结论是 :(1)土壤种子库密度随沙漠化程度的增加而下降 ,不同沙漠化发展阶段下降速率不同 ,从固定到半固定沙地是种子库密度衰减最快的时期 ;(2 )土壤种子库植物种数从固定到半流动沙地变化很小 ,半流动到流动沙地衰减速度明显加快 ,是种子库植物种数衰减最快的时期 ;(3) 4种类型退化沙地土壤种子库的种类组成以 1、2年生草本植物为主 ,所占比例 79%～ 88% ,多年生草本和灌木所占比例很小 ;(4 )土壤种子库物种多样性指数并非随着沙漠化程度的增加而下降 ,其中半流动沙地的物种多样性指数最小 ;(5 ) 4种类型退化沙地土壤种子库组成上的相似性系数变化范围为 0 .82 9～ 1.0 0 0 ,表现出较小的空间异质性。     

干旱区矿区不同立地类型土壤种子库特征

矿区的自然或人工植被恢复是维持生物多样性及生态系统稳定的重要途径。通过室外萌发法对金昌镍铜矿区7种不同立地生境土壤种子库的研究表明:该区域土壤中物种丰富度较高,包括16科47属,共56种植物,分别为:藜科(Chenopodiaceae)、禾本科(Gramineae)、菊科(Compositae)、柽柳科(Tamaricaceae)、苋科(Amaranthaceae)、马齿苋科(Portulacaceae)、豆科(Leguminosae)、十字花科(Cruciferae)、车前科(Plantaginaceae)、胡颓子科(Elaeagnaceae)、蒺藜科(Zygophyllaceae)、莎草科(Cyperaceae)、大戟科(Euphorbiaceae)、紫草科(Boraginaceae)、蔷薇科(Rosaceae)、茄科(Solanaceae);植物组成以一年生草本植物占绝对优势(89.3%),多年生草本植物次之(7.54%),灌木种最少(2.59%)。土壤种子平均储量412粒·m~(-2),不同立地类型土壤种子库储量差异显著,与其他荒漠地区比较,土壤种子库储量偏低;土壤种子库物种Simpson多样性指数为:新尾矿周边自然植被区龙首矿自然植被区龙首矿开采废弃地供暖公司柽柳林地。     

不同干扰条件下荒漠区土壤种子库

在内蒙古阿拉善荒漠区选择开垦、轮牧、过牧和禁牧4种不同干扰条件的样区,于2005年4月(春)、7月(夏)和10月(秋)分别对土壤种子库取样,并在实验室条件下进行发芽试验,得出如下结果:(1)4种不同干扰区在春、夏、秋三个季节土壤种子库可萌发种子平均数量分别为,开垦干扰:688粒/m2;轮牧干扰:1328粒/m2;过牧干扰:1488粒/m2;禁牧干扰:944粒/m2;(2)开垦区与禁牧区在4月份(春)、7月份(夏)、10月份(秋)土壤种子库可萌发种子数量呈连续上升趋势,而轮牧区与过牧区则呈先上升后下降趋势;(3)4种不同干扰条件下,开垦区、过牧区和禁牧区在不同深度土层中水分含量均无显著差异;而轮牧区0-5cm与其它三个土层中水分含量出现显著差异,而且0-5cm土壤层的水分含量与种子的萌发数量呈显著正相关。     

短花针茅荒漠草原土壤种子库对不同放牧强度的响应

在内蒙古短花针茅荒漠草原,采用野外随机定点取样与室内萌发相结合的方法,对不同放牧强度下围封草地土壤种子库的密度、垂直结构、多样性、丰富度、均匀度、以及种子库与地上植被的相似性进行了研究。结果表明,在各试验区土壤种子库主要分布在0～20cm土层,占种子库总密度的87%～96%;随着放牧强度的增加,土壤种子库总密度减少,同时地上植被与土壤种子库的相似物种数减少,种子库组成的相异性增加;轻度放牧对土壤种子库中一二年生草本所占比例影响较小,而中度和重度放牧使土壤种子库中一二年生草本所占比例增加;土壤种子库的物种丰富度、多样性、均匀度均随放牧强度的增加而减小。     

Dermestid beetles in ‘evolution Canyon’, lower Nahal Oren, Mt. Carmel, including new records for Israel

Fifteen species of dermestid beetles were recorded at ‘Evolution Canyon’ (EC), Lower Nahal Oren, Mt. Carmel, Israel. They represent ~35% of known Israeli dermestid species. The following three species were recorded for the first time in Israel:Trogoderma svriaca Dalla Torre, 1911;Ctesias svriaca Ganglbauer, 1904; andAnthrenus (s.str.) jordaniens Pic, 1934. Adults of 13 species were collected on the more solar radiated, warmer and climatically more fluctuating south-facing slope (SFS); ten species were collected on the opposite, north-facing slope (NFS), which was cooler and climatically more stable. The abundance of adult dermestid beetles was 1.9 times higher on the SFS than on the NFS (86 and 47, respectively). Species richness and abundance distribution at EC (three collecting stations on each slope and one at the valley bottom) were significantly negatively correlated with the plant cover that consisted of trees and bushes (Spearmanr _s ,P=0.007 and 0.039, respectively) and perennials (Spearmanr _s ,P=0.039 and 0.077, respectively), indicating that non-woody plants were preferred by adult dermestid beetles.     

Epidemiology of Toxigenic Fungi and their Associated Mycotoxins for Some Mediterranean Crops

Recent data on the epidemiology of the common mycotoxigenic species of Fusarium, Alternaria, Aspergillus and Penicillium in infected or colonized plants, and in stored or processed plant products from the Mediterranean area are reviewed. Emphasis is placed on the toxigenicity of the causal fungal species and the natural occurrence of well known mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, zearalenone, patulin, Alternaria-toxins and moniliformin), as well as some more recently described compounds (fusaproliferin, beauvericin) whose toxigenic potential is not yet well understood. Several Fusarium species reported from throughout the Mediterranean area are responsible of the formation of mycotoxins in infected plants and in plant products, including: Fusarium graminearum, F. culmorum, F. cerealis, F. avenaceum, F. sporotrichioides and F. poae, which produce deoxynivalenol, nivalenol, fusarenone, zearalenone, moniliformin, and T-2 toxin derivatives in wheat and other small grains affected by head blight or scab, and in maize affected by red ear rot. Moreover, strains of F. verticillioides, F. proliferatum, and F. subglutinans, that form fumonisins, beauvericin, fusaproliferin, and moniliformin, are commonly associated with maize affected by ear rot. Fumonisins, were also associated with Fusarium crown and root rot of asparagus and Fusarium endosepsis of figs, caused primarily by F. proliferatum. Toxigenic A. alternata strains and associated tenuazonic acid and alternariols were commonly found in black mould of tomato, black rot of olive and citrus, black point of small cereals, and black mould of several vegetables. Toxigenic strains of A. carbonarius and ochratoxin A were often found associated with black rot of grapes, whereas toxigenic strains of A. flavus and/or P. verrucosum, forming aflatoxins and ochratoxin A, respectively, were found in moulded plant products from small cereals, peanuts, figs, pea, oilseed rape, sunflower seeds, sesame seeds, pistachios, and almonds. Finally, toxigenic strains of P. expansum and patulin were frequently found in apple, pear and other fresh fruits affected by blue mould rot, as well as in derived juices and jams.     

A survey of the genera of<Emphasis Type="Italic">Microgaster</Emphasis> latreille and<Emphasis Type="Italic">Hygroplitis</Emphasis> thomson (Hymenoptera: Braconidae: Microgastrinae) from China

The genera ofMicrogaster Latreille 1804 andHygroplitis Thomson 1895 from China are presented systematically in this paper. Thirty-two species ofMicrogaster and three species ofHygroplitis are known in China. Diagnosis, character variation, distribution and host of each species among the two genera are presented, including its host and distribution. Keys to the species ofMicrogaster andHygroplitis are given. http://www.phytoparasitica.org posting Dec. 19, 2006.     

Plant Viruses Transmitted by Whiteflies

One-hundred and fourteen virus species are transmitted by whiteflies (family Aleyrodidae). Bemisia tabaci transmits 111 of these species while Trialeurodes vaporariorum and T. abutilonia transmit three species each. B. tabaci and T. vaporariorum are present in the European–Mediterranean region, though the former is restricted in its distribution. Of the whitefly-transmitted virus species, 90% belong to the Begomovirus genus, 6% to the Crinivirus genus and the remaining 4% are in the Closterovirus, Ipomovirus or Carlavirus genera. Other named, whitefly-transmitted viruses that have not yet been ranked as species are also documented. The names, abbreviations and synonyms of the whitefly-transmitted viruses are presented in tabulated form together with details of their whitefly vectors, natural hosts and distribution. Entries are also annotated with references. Whitefly-transmitted viruses affecting plants in the European–Mediterranean region have been highlighted in the text.     

Broad bean mottle virus in Morocco; curculionid vectors,and natural occurrence in food legumes other than faba bean (Vicia faba)

Broad bean mottle virus (BBMV) was transmitted from infected to healthy faba-bean plants by the curculionid weevilsApion radiolus Kirby,Hypera variabilis Herbst,Pachytychius strumarius Gyll,Smicronyx cyaneus Gyll, andSitona lineatus L. The latter appeared to be an efficient vector: acquisition and inoculation occurred at the first bite, the rate of transmission was c. 41%, and virus retention lasted for at least seven days.S. lineatus transmitted the virus from faba bean to lentil and pea, but not to the three genotypes of chickpea tested. This is the first report on the generaHypera, Pachytychius, andSmicronyx as virus vectors, and onA. radiolus, H. variabilis, P. strumarius, andS. cyaneus as vectors of BBMV.Out of 351 samples of food legumes with symptoms suggestive of virus infection, 16, 11, 19, and 17% of the samples of chickpea, lentil, pea, and common bean, respectively, were found infected when tested for BBMV in DAS-ELISA. This is the first report on the natural occurrence of BBMV in chickpea, lentil, pea, and common bean. The virus should be regarded as a food-legume virus rather than a faba-bean virus solely, and is considered an actual threat to food legume improvement programmes.     

Characterization of potato potyvirus Y (PVY) isolates from seed potato batches. Situation of the NTN, Wilga and Z isolates

A collection of 38 PVY isolates from seed potato batches, originating from several Western European countries, was characterized by using current biological, serological and molecular tools differentiating PVY strains and groups. The correlation between the three kinds of tests was good but not absolute. No single serological or PCR method was able to discriminate among the five isolate groups found. Twenty-nine isolates belonged to the PVY^N strain and six to the PVY^O strain. No PVY^C was found. Two other isolates reacted serologically like PVY^O, but were unable to elicit a hypersensitive response from the Ny_tbr gene and probably represent the PVY^Z group. At the molecular level, these two isolates showed a combination of both PVY^O and PVY^N and could be recombinants of these strains. Another isolate reacted serologically like PVY^O, but induced vein necrosis in tobacco, like PVY^N-Wilga. Some PVY^N isolates caused tuber ring necrosis in glasshouse conditions. These might belong to the PVY^NTN group. The PVY^NTN, PVY^N-Wilga and PVY^Z groups probably represent pathotypes within strains PVY^N and PVY^O, respectively. The present study also confirms previous reports showing a high genetic variation at the 5 end within the PVYN strain.     

The autumn leafroller: Phenology,damage and parasitoids in a Dutch apple orchard

The phenology of the autumn leafroller,Syndemis musculana, a local pest of apple, was studied in order to forecast larval emergence. From 1983–1986, peak flight as determined with sexpheromone traps was always between 13–18 May. The duration of embryonic development was determined at various constant temperatures and used to estimate the periods of egg hatch in these four years. Each year, most eggs should have hatched in the second decade of June.Differences in attack rates between apple cultivars seem to be explained largely by the variation in picking time. Larvae are only half grown at the beginning of harvest (cv. James Grieve), and have gone into hibernation when the latest variety (cv. Golden Delicious) is picked. Moreover, the varieties Cox's Orange Pippin and Belle de Boskoop, picked about half time, are liable to receive additional damage by caterpillars brought with the picked fruits into storage.Various hymenopterous parasites were reared from caterpillars. As the only leafroller in the orchard which hibernates as mature larva,S. musculana may promote winter survival of some parasitoids, like the eulophidColpyclypeus florus.Samenvatting De fenologie van de herfstbladroller (Syndemis musculana Hübner), een incidentele plaag op appel, werd nader bepaald met het doel het uitkomen van de eieren te kunnen voorspellen. In 1983–1986 viel de piekvlucht, bepaald met behulp van feromoonvallen, steeds tussen 13 en 18 mei.De ontwikkelingsduur van de eieren bij verschillende constante temperaturen werd gebruikt om de periode van uitkomen te schatten. De meeste eieren zullen ieder jaar in de eerste helft van juni uitkomen.Geconstateerde verschillen in schade tussen appelrassen blijken goeddeels terug te voeren op verschillen in pluktijdstip. De rupsen van de herfstbladroller zijn pas half-was als de eerste appels eind augustus geplukt worden, terwijl tegen het einde van de oogst begin oktober de meeste al in winterslaap zijn. Met name tussentijdse rassen, als Cox's Orange Pippin and Schone van Boskoop, lopen extra schade op doordat grotere rupsen met de geplukte vruchten in de kist terecht komen.Uit de rupsen werden negen, al van andere boomgaardbladrollers bekende, sluipwespen gekweekt, Omdat deze bladrollersoort, als enige in de boomgaard, als volgroeide rups overwintert, lijkt zij bij uitstek geschikt als winterwaard.This study was carried out at the Experimental Orchard De Schuilenburg, Schuilenburg 3, 4041 BK Kesteren, the Netherlands, to which address correspondence should be addressed.     

Diagnostics,genetic diversity and pathogenic variation of ascochyta blight of cool season food and feed legumes

Molecular diagnostic techniques have been developed to differentiate the Ascochyta pathogens that infect cool season food and feed legumes, as well as to improve the sensitivity of detecting latent infection in plant tissues. A seed sampling technique was developed to detect a 1% level of infection by Ascochyta rabiei in commercial chickpea seed. The Ascochyta pathogens were shown to be genetically diverse in countries where the pathogen and host have coexisted for a long time. However, where the pathogen was recently introduced, such as A. rabiei to Australia, the level of diversity remained relatively low, even as the pathogen spread to all chickpea-growing areas. Pathogenic variability of A. rabiei and Ascochyta pinodes pathogens in chickpea and field pea respectively, appears to be quantitative, where measures of disease severity were based on aggressiveness (quantitative level of infection) rather than on true qualitative virulence. In contrast, qualitative differences in pathogenicity in lentil and faba bean genotypes indicated the existence of pathotypes of Ascochyta lentis and Ascochyta fabae. Therefore, reports of pathotype discrimination based on quantitative differences in pathogenicity in a set of specific genotypes is questionable for several of the ascochyta-legume pathosystems such as A. rabiei and A. pinodes. This is not surprising since host resistance to these pathogens has been reported to be mainly quantitative, making it difficult for the pathogen to overcome specific resistance genes and form pathotypes. For robust pathogenicity assessment, there needs to be consistency in selection of differential host genotypes, screening conditions and disease evaluation techniques for each of the Ascochyta sp. in legume-growing countries throughout the world. Nevertheless, knowledge of pathotype diversity and aggressiveness within populations is important in the selection of resistant genotypes.     

Characterization of Sclerotinia minor populations in Texas peanut fields

In the summer of 2004 an epidemic of sclerotinia blight of peanut, a disease caused by Sclerotinia minor, occurred in Texas in fields where the disease was never previously detected. The disease was observed on many plants within one of the fields (>3000 disease foci), although most foci were <1 m. It is hypothesized that these observations were inconsistent with the recent introduction of a monocyclic pathogen, even if disease developed under conducive environmental conditions. The pattern of disease is most suggestive of the presence of foliar (ascospore) infections, although air temperature was above the known limits for apothecia development if the pathogen had arrived in the field in 2004 peanut seed. To further examine this epidemic, 232 isolates were collected, across a variety of spatial scales spanning this field and other Texas peanut fields, and evaluated for aggressiveness, fungicide sensitivity and genotypic diversity. There was wide variation among isolates for the phenotypic characteristics measured, but there was no evidence that a genotypically unique, highly aggressive, and fungicide resistant isolate had been introduced or evolved. The predominant genotype, TX1, which contained 154 isolates, was found in every county and field population.     

Tumours of Begonia and some other ornamentals,induced by Corynebacterium fascians

In 1975 many tumours were observed in plants ofBegonia Schwabenland grown in Aalsmeer. Submersion of the roots ofNicotiana megalosiphon seedlings in a homogenate of tumorous tissue, induced tumours after two weeks. Short periods of submergence yielded results similar to those obtained after longer periods. Tumour homogenates lost their infectivity after ten min at 50°C. Aphids transmitted the infectious agent.Treatment with propylene oxide did not inhibit infectivity completely. Filtration through a 450 nm filter removed the infectious agent.Tobacco tumor virus or a viroid could not be isolated. Cultures ofCorynebacterium fascians, isolated from tumours ofN. megalosiphon were highly infectious and induced tumours in healthyN. megalosiphon andBegonia. Tumorous tissue homogenates ofPelargonium zonale, Dahlia sp.,Gladiolus sp., andLilium sp. also caused tumours inN. megalosiphon, from whichC. fascians was isolated. It was not possible to produce tumours inN. megalosiphon with homogenates from roses with symptoms of bud proliferation.Samenvatting In 1975 werden vele tumoren waargenomen inBegonia Schwabenland op Aalsmeerse bedrijven (Fig. 1). De infectiositeit van tumorweefsel kon goed en snel worden vastgesteld door de wortels van zaailingen vanNicotiana megalosiphon in een homogenaat van tumorweefsel te dompelen. Tumoren ontstonden na twee weken, de eindbeoordeling geschiedde na een maand (Fig. 2). Ook verschillende andereNicotiana spp.,Melilotus officinalis (Fig. 3) enPisum odoratum (Fig. 4) werden aangetast.Bij de infectiositeitstoets gaven zeer korte dompeltijden even goede resultaten als langere (Tabel 1). Infectieus sap verloor zijn infectievermogen na 10 min verhitting bij 50°C. Bladluizen brachten de smetstof over. Propyleenoxide verminderde de infectiositeit wel, doch onderdrukte deze niet totaal. Bij filtratie door een 450 nm filter bleef het infectieuse agens op het filter achter. Het tumor-inducerende agens was ook aanwezig in die delen van planten met tumoren welke gezond leken en het ging voor een gering deel over met zaad (Tabel 2).Uit tumoren konden wij geen tabakstumorvirus of een viroïde isoleren. Culturen vanCorynebacterium fascians, geïsoleerd uit tumoren vanN. megalosiphon bleken zeer infectieus en veroorzaakten tumoren inN. megalosiphon enBegonia. Homogenaten van tumorweefsel vanPelargonium zonale, dahlia (Fig. 5), gladiool (Fig. 6) enLilium Mid Century Hybrid Enchantment (Fig. 7) veroorzaakten ook tumoren opN. megalosiphon, waaruitC. fascians werd geïsoleerd. Met sap van kroeskopzieke rozen konden wijN. megalosiphon niet besmetten.