石灰性土壤供氮能力几种化学测定方法的评价研究

以采自于黄土高原差异较大的25个农田耕层石灰性土壤为供试土样,以淋洗和未淋洗土壤起始NO3--N小麦和玉米两季盆栽试验作物累积吸氮量为参比,对8种反映旱地土壤供氮能力的化学方法进行比较研究。结果表明,在一定程度上,可用有密切关系的土壤全氮或有机质,反映有机氮或全氮存在较大差异的土壤供氮能力,但其灵敏性较差。石灰性土壤矿质氮,特别是NO3--N与未淋洗土壤起始NO3--N作物吸氮量之间有较高相关性(r=0.884,P0.01),而与淋洗土壤起始NO3--N作物吸氮量间相关系数仅为0.472(P0.05),说明矿质氮可反映土壤当前供氮水平,而不能反映土壤潜在供氮能力;石灰性土壤起始NO3--N对各化学方法与作物吸氮量之间相关性影响较大。酸性高锰酸钾法既可反映土壤潜在供氮能力,也可反映土壤总供氮能力;酸性高锰酸钾法的修订方法,即硫酸高锰酸钾法提取出的NH4+-N值接近于KCl水浴法提取出的NH4+-N值,该方法在反映土壤总供氮能力方面与酸性高锰酸钾法相当,但在反映土壤潜在供氮能力方面不及酸性高锰酸钾法优越。KCl水浴法在评价石灰性土壤供氮能力方面,与酸性高锰酸钾法的效果基本相同;沸水浸取法和NaHCO3-UV法在评价石灰性土壤供氮能力方面效果较差。总结以上发现,在以盐类溶液提取法中,酸性高锰酸钾法、硫酸高锰酸钾法和KCl水浴法可作为反映石灰性土壤供氮能力的化学方法,其中以酸性高锰酸钾法最优,其次为KCl水浴法和硫酸高锰酸钾法。这3种方法在不包括起始NO3--N时,可反映石灰性土壤潜在供氮能力;包括起始NO3--N后,可反映土壤总供氮能力(当前供氮能力+潜在供氮能力)。     

对间隙淋洗长期通气培养条件下黄土高原土壤供氮能力的评价

采用间隙淋洗长期通气培养法,通过对黄土高原物理化学性质差异较大的10种农田土样起始矿质氮、起始提取态总氮、起始可溶性有机氮,以及培养期间淋洗矿质氮、淋洗总氮、可溶性有机氮含量及其与作物吸氮量关系的研究,分析并评价黄土高原主要农田土壤氮素矿化能力以及包括和不包括培养淋洗可溶性有机氮对土壤供氮能力的影响。结果表明,供试土样起始可溶性有机氮平均为N 23.9 mg/kg,是起始提取态总氮的28.8%,土壤全氮的2.4%。在通气培养淋洗总氮中,可溶性有机氮所占比例不高,经过217 d通气培养,淋洗出的可溶性有机氮平均为N 28.8mg/kg,占淋洗总氮量的19.8%。相关分析表明,淋洗可溶性有机氮量与第1季作物吸氮量相关不显著,但与连续2季作物总吸氮量显著相关。淋洗矿质氮、淋洗总氮与两季作物总吸氮量的相关系数明显高于与第一季作物吸氮量的相关系数;与第一季作物吸氮量达显著相关水平,与连续两季作物吸氮量达极显著相关水平。总体上看,可溶性有机氮和土壤全氮、土壤微生物氮不能作为反映短期可矿化氮的指标;间隙淋洗通气培养淋洗液中淋洗矿质氮、淋洗总氮是评价可矿化氮的较好指标,不仅适宜于第一季作物,而且也适用于对连续两季作物土壤供氮能力的评价。     

稻田土壤氮素矿化的几种方法比较

结合盆栽实验,对稻田土壤氮素矿化的几种方法进行了比较,结果表明:KC I煮沸法和碱解氮法是很好的测试土壤供氮能力的方法,淹水培养法矿化氮量与植物全氮含量相关性不高;而采用淹水培养间歇淋洗法和淹水密闭连续培养法对稻田土壤进行土壤氮素矿化的淋溶试验,结果表明:淋洗有利于土壤氮素的矿化过程。     

土壤供氮能力指标研究

本文对国内外土壤供氮能力指标的研究进行了论述 ,作者根据自己的研究结果认为 :在土壤起始NO- 3 N含量较高时 ,用KCl煮沸浸取出的一定深度土壤的NO- 3 N就可作为土壤供氮能力指标 ,反映出当前土壤的供氮能力 .而在土壤起始NO- 3 N含量较低或考虑到起始NO- 3 N的影响后 ,1mol/LNaOH扩散出的NH+ 4 N或KCl煮沸法在酸性土壤上浸取出的NH+ 4 N则是较好的土壤供氮能力指标 ,反映了土壤潜在的供氮能力     

施肥对日光温室黄瓜和土壤硝酸盐含量的影响

通过田间试验研究了不同施肥对日光温室黄瓜NO2--N和NO3--N含量和土壤NO3--N以及黄瓜产量的影响。结果表明,在黄土高原黄绵土上,施N400kg.hm2和P2O5250kg.hm2,黄瓜生长期间,NO3--N含量变化与黄瓜的生长发育阶段关系密切,黄瓜结瓜前020和2040cm土层NO3--N含量较高,随黄瓜生长速度加快和结瓜盛期的到来,土壤NO3--N含量降低;黄瓜收获后,NO3--N含量又有增加。不同施肥种类比较,施用化肥40160cm土层NO3--N的累积和淋洗量最大,施用沼肥其累积和淋洗量小于施用化肥,而施用有机肥(牛粪)NO3--N的累积和淋洗量小于施用沼肥。采用叶面喷施尿素和有机钾肥,可以减少化肥和有机肥用量,从而降低土壤剖面0200cmNO3--N的累积。使用沼肥、叶面肥的黄瓜产量都明显高于不施肥和NP化肥处理。     

氮肥投入水平对蔬菜地硝态氮淋洗特征的影响

通过3年的田间定位试验研究了不同施N条件下蔬菜地NO3--N淋洗浓度及淋洗量的变化。结果表明:在农民习惯的传统施N处理(花椰菜为450kg/hm2,苋菜为100kg/hm2,菠菜为309kg/hm2)下蔬菜地NO3--N的淋洗浓度明显高于2个优化施N处理下蔬菜地的NO3--N淋洗浓度。在花椰菜、苋菜和菠菜生长期内,2个优化施N处理下蔬菜地NO3--N平均淋洗量分别是传统施N处理下蔬菜地NO3--N平均淋洗量的19%、18%、9%和13%、34%、21%。试验期间传统施N处理下蔬菜地NO3--N年季平均累积淋洗量约占年季平均施N量的一半,其中休闲期NO3--N平均累积淋洗量占年季平均累积施N量的20%;而其他2个优化施N处理NO3--N年季平均累积淋洗量是年季平均累积施N量的27%,休闲期NO3--N平均淋洗量占年季平均累积施N量的6%,而处理间蔬菜产量并未受到明显影响。     

不同施氮量对太湖地区新增设施菜地土壤硝态氮累积的影响

采用田间小区试验,研究了太湖地区大棚蔬菜不同施N量对土壤NO3--N累积及其土壤电导率、pH和蔬菜产量品质的影响.结果表明:土壤NO3--N的累积与施N量呈正比,种植两季蔬菜后,农民习惯施N下的土壤NO3--N从35 mg/kg上升到221.35 mg/kg;土壤电导率、pH与NO3--N含量显著相关,其中电导率与NO3--N的关系更为密切,相关系数达到0.832.NO3--N的累积导致蔬菜的产量与品质下降,与莴苣、芹菜施N量分别为312、384 kg/hm2的处理相比,农民习惯施N处理下的莴苣和芹菜产量分别下降了11.8％和33.9％,Vc含量下降了17％和36.6％,而硝酸盐含量增加了22.3％和32％,农民习惯施N处理下的两季蔬菜硝酸盐含量均达到三级污染,可危害人体健康.     

施用磷肥对土壤NO3——N累积的影响

在黄土高原南部的国家黄土肥力和肥料效益监测基地进行的长期定位试验结果表明 ,在小麦 玉米轮作中 ,当年施氮量为N 352kg/hm2 时 ,单施氮肥或氮钾配合的 0～4m土壤剖面的NO3--N累积量达 1000kg/hm2 以上 ,其中约 50%～60%的NO3--N分布在 2～ 4m以下的土层中 ,而氮磷配合的 0～ 4m土壤剖面的NO3--N累积量仅为 220kg/hm2,且 80 %的NO3--N分布在 0～2m的土层中 ,增施磷肥由于增加了氮的吸收和对水分的利用而有效地降低了土壤中NO3--N的累积。     

污水灌溉对稻田土壤氮磷淋失动态变化的影响

通过模拟稻田灌溉大型淋洗柱试验,在污染河水灌溉条件下对太湖地区水稻生长季两种主要类型的稻田土壤--黄泥土和乌珊土的氮磷淋洗特征进行了研究.结果表明,在灌溉淹水初期,不同形态氮素的淋失量均比较高,并达到峰值,以后淋失量逐渐降低,说明淹水初期淋失的氮素不是来源于灌溉河水,而是主要来自土壤氮.到淹水后期,NO3--N和NH4 -N淋失量接近零值,但仍能观测到可溶性有机氮淋失现象,这表明可溶性有机氮是污水灌溉稻田土壤主要的氮素淋失形态.而磷素的淋失动态与氮素的淋失动态截然相反,在灌溉淹水后很长一段时间内均观测不到土壤磷素淋失,但在淹水灌溉的淹水后期,发现土壤磷素有淋溶损失现象,这可能是利用富营养化的河水长期淹水后,土壤对磷的吸持已达到饱和状态,土壤不能继续固持多余的磷素所致.     

灌溉对土壤硝态氮淋吸效应影响的研究

在陕北米脂县无定河谷地沙壤质土壤上进行了灌水量对土壤硝态氮的淋失和作物吸收效应影响的研究( 简称淋吸效应) 。结果表明,灌水量在0～4000m3/hm2范围内,与玉米产量和玉米吸N 量之间的关系均呈线性相关。土壤剖面中NO3--N 遗留量主要集中分布在0 ～60cm土层内,出现的高峰在40cm ;在0 ～80cm 土层内的NO3--N 遗留量随灌水量的增加而降低;80 ～320cm 土层内的NO3--N 与灌水量之间无明显相关,320 ～400cm 土层内NO3--N 是随灌水量的增加而增高。不同深度的土壤剖面中NO3--N 遗留量与灌水量之间均呈双曲线相关;氮素损失率以未灌溉和灌水量4000m3/hm2处理的为最低,据此提出了土壤NO3--N 淋吸效应的概念。     

Comparative Study on Disturbed and Undisturbed Soil Sample Incubation for Estimating Soil Nitrogen-Supplying Capacity

Application of nitrogen (N) fertilizers without knowing the N-supplying capacity of soils may lead to low N use efficiency, uneconomical crop production, and pollution of the environment. Based on the results from pot experiments treated with soil initial nitrate leaching and native soil, long-term alternate leaching aerobic incubation was conducted to study the disturbed and undisturbed soil N-supplying capacity of surface soil samples in 11 sites with different fertilities on the Loess Plateau. The results indicated that the entire indexes and ryegrass (Lolium perenne) uptake N with soil initial nitrate leaching showed a better correlation than that without soil initial nitrate leaching. Except the correlation coefficients for soil initial nitrate (NO₃ ^?)-N and mineral N extracted by calcium chloride (CaCl₂) before aerobic incubation with ryegrass uptake without soil initial nitrate leaching, the correlation coefficients for soil initial NO₃ ^?-N and mineral N extracted by CaCl₂ before aerobic incubation with ryegrass uptake with soil initial nitrate leaching and those for mineralizable N extracted by aerobic incubation, soil initial mineral N and mineralizable N extracted by aerobic incubation, potentially mineralizable N (N₀) and soil initial mineral N + N₀ with ryegrass uptake N under the two cases in disturbed treatment were all higher than those in undisturbed treatment. We concluded that NO₃ ^?-N in soil extracted by CaCl₂ before aerobic incubation can reflect soil N-supplying capacity but cannot reflect soil potential N-supplying capacity. Without soil initial nitrate leaching, the effect of disturbed and undisturbed soil samples incubated under laboratory conditions for estimating soil N-supplying capacity was not good; however, with soil initial nitrate leaching, this method could give better results for soil N-supplying capacity. Based on the results from pot experiments treated with soil initial nitrate leaching and native soil, the mineralization of disturbed soil samples can give provide better results for predicting soil N-supplying capacity for in situ structure soil conditions on the Loess Plateau than undisturbed soil samples.     

Boiling Potassium Chloride–Extractable Nitrogen as an Index of Potentially Mineralizable and Plant‐Available Nitrogen in Soil

Abstract

Mineralization of soil organic nitrogen (N) and its contribution toward crop N uptake is central to developing efficient N‐management practices. Because biological incubation methods are time consuming and do not fit into the batch‐analysis techniques of soil‐testing laboratories, an analytical procedure that can provide an estimate of the mineralizable N would be useful as a soil‐test method for predicting plant‐available N in soil. In the present studies, the ability of boiling potassium chloride (KCl) to extract potentially mineralizable and plant‐available N in arable soils of semi‐arid India was tested against results from biological incubations and uptake of N by wheat in a pot experiment. Mineralization of organic N in soils was studied in the laboratory by conducting aerobic incubations for 112 days at 32°C and 33 KPa of moisture. Cumulative N mineralization in different soils ranged from 8.2 to 75.6 mg N kg^?1 soil that constituted 2.7 to 8.8% of organic N. The amount of mineral N extracted by KCl increased with increase in length of boiling from 0.5 to 2 h. Boiling for 0.5, 1, 1.5, and 2 h resulted in an increase in mineral‐N extraction by 9.3, 12.7, 19.6, and 26.1%, respectively, as compared to mineral N extracted at room temperature. The boiling‐KCl‐hydrolyzable N (ΔN_i) was directly dependent upon soil organic N content, but the presence of clay retarded hydrolysis for boiling lengths of 0.5 and 1 h. However, for boiling lengths of 1.5, and 2 h, the negative effect of clay was not apparent. The ΔN _i was significantly (P=0.05) correlated to cumulative N mineralized and N‐mineralization potential (N₀). The relationship between N₀ and ΔN _i was curvilinear and was best described by a power function. Boiling length of 2 h accounted for 78% of the variability in N₀. Results of the pot experiment showed that at 21‐ and 63‐day growth stages, dry‐matter yield and N uptake by wheat were significantly correlated to boiling‐KCl‐extractable mineral N. Thus, boiling KCl could be used to predict potentially mineralizable and plant‐available N in these soils, and a boiling time of 2 h was most suitable to avoid the negatively affected estimates of boiling‐KCl‐hydrolyzable N in the presence of clay. The results have implications for selecting length of boiling in soils varying widely in clay content, and this may explain why, in earlier studies, longer boiling times (viz. 2 or 4 h) were better predictors of N availability as compared to 0.5 and 1 h.     

Winter cover cropping influence on nitrogen mineralization,presidedress soil nitrate test,and corn yields

The mineralization and availability of cover crop N to the succeeding crop are critical components in the management of soil N to reduce N leaching. The effects of several leguminous and non-leguminous cover crops on soil N availability, N mineralization potential, and corn (Zea mays L.) yield were examined. The cover crops had variable effects on soil N availability and corn yield and N uptake. Because of the rapid mineralization of the cover crops following incorporation, the inorganic N levels in the soil sampled in mid-May 1992 (4 weeks after incorporation of cover crops), rather than the potentially mineralizable N, rate constants, initial potential mineralization rate, or cumulative N mineralized over 14 weeks, correlated well with N concentrations, C:N ratios, or the N added in the cover crops. However, the inclusion of potentially mineralizable N with inorganic N in a multiple regression improved the variability in the corn yield and the N uptake accounted for. Since extensive mineralization had occurred before the 21 May sampling, the potentially mineralizable N was affected more by the soil organic N and C than by the N concentrations of the cover crops. The presidedress NO₃ ^--N test levels were well predicted by the inorganic and potentially mineralizable N (R ²=0.89, P<0.01), although the test levels were better in predicting corn yield and N uptake. If the available soil N test needs to be made earlier than recommended by the presidedress NO₃ ^--N test, both inorganic and potentially mineralizable N are needed to better predict the corn yield and N uptake in the soils.     

北京地区潮土表层中NO3--N的转化积累及其淋洗损失

本试验利用渗滤池设施，采用化学分析和同位素技术相结合的方法研究了北京地区潮土表层中施用氮肥后ＮＯ３＾－－Ｎ转化积累及其１３０ｃｍ土体的淋洗状况。常规分析结果表明，在春小麦和夏玉米的生育前期可以观察到氮素明显地向ＮＯ３＾－－Ｎ的转化积累，其强度随尿素施用量的增加而明显增加，而尿素、硝铵、硫铵等不同氮肥品种处理之间有差异但大多不显著。同时夏玉米期间转化积累作用比春小麦期间强烈。＾１５Ｎ标记试验结果表明     

Comparison of Chemical Methods for Assessing Nitrogen Mineralization in Two Calcareous Soils Treated with Organic Materials

Reliable and quick methods for measuring nitrogen (N)–supplying capacities of soils (NSC) are a prerequisite for using N fertilizers. This study was conducted to develop a routine method for estimation of mineralizable N in two calcareous soils (sandy loam and clay soils) treated with municipal waste compost or sheep manure. The methods used were anaerobic biological N mineralization, mineral N released by 2 M potassium chloride (KCl), ammonium (NH₄ ⁺) N extracted by 1 N sulfuric acid (H₂SO₄), NH₄ ⁺-N extracted by acid potassium permanganate (KMnO₄), and NH₄ ⁺-N released by oxidation of soil organic matter using acidified potassium permanganate. The results showed that oxidizable N extracted by acid permanganate, a simple and rapid measure of soil N availability, was correlated with results of the anaerobic method. Oxidative 0.05 N KMnO₄ was the best method, accounting for 78.4% of variation in NSC. Also, the amount of mineralized N increased with increasing level of organic materials and was greater in clay soil than sandy loam soil.     

控释氮肥养分控释效果及合理施用研究

试验采用{3,3}单形重心设计方法,研究了普通尿素和2种包膜尿素D90、D60配比对土壤NH4+-N、NO3--N及矿质态氮(Nmin)含量的影响。结果表明,供试的7种包膜肥料初期溶出率均12.0%,微分溶出率在0.26%～2.49%之间。各处理土壤NH4+-N含量均随时间逐渐降低,而NO3--N和Nmin含量随时间逐渐增加。整个培养期内单独施用尿素处理,土壤NH4+-N、NO3--N及Nmin含量最高;2种控释肥单施或其配比施用土壤NH4+-N、NO3--N及Nmin含量最低;尿素与控释肥配合施用,土壤NH4+-N、NO3--N及Nmin含量居中。不同时期内土壤NH4+-N的来源不同,0～20d内,尿素对土壤NH4-N含量贡献最大;30～50d内,土壤NH4+-N主要来自D60;整个培养期内尿素对土壤NO3--N和Nmin的贡献均最大。肥料配比中随着尿素比例的减少,土壤NH4+-N、NO3--N及Nmin均逐渐减少。研究结果初步验证了混料设计在肥料配比研究中的可行性。     

Extractable nitrogen using hot potassium chloride as a mineralization potential index

Inorganic nitrogen (N) in soils is a primary component of soil‐plant N buffering. This study was conducted to determine if non‐exchangeable ammonium‐nitrogen (NH₄‐N) could serve as an index of potentially mineralizable organic N which is an important sink in N buffering. Four long‐term winter wheat (Triticum aestivum L.) experiments that had received annual fertilizer N at 0 to 272 kg N ha^‐1 were used. Soils from these experiments were extracted by four 10 mL portions of 2M potassium chloride (KC1) at room temperature followed by extraction with 20 mL of 2M hot KC1. Extraction at 100°C for four hours using 3 g soil and 20 mL 2M KC1 was found to be the most effective. Hot KC1‐extractable NH₄‐N minus room temperature KCl‐extractable NH₄‐N was considered non‐exchangeable NH₄‐N. Non‐exchangeable NH₄‐N was correlated with the long‐term N rates, and believed to be a reliable index of potentially mineralizable organic N. The relationship was linear for NH₄‐N where the lowest N rate had the lowest extractable N. The mean non‐exchangeable NH₄‐N concentration ranged from 8.42 to 16.34 mg kg^‐1; whereas, nitrate‐nitrogen (NO₃‐N) ranged from 0.07 to 1.87 mg kg¹. Total inorganic N extracted was similar to that mineralized in a 42‐day aerobic water saturated incubation. In addition, using a linear‐plateau model, extractable NH₄‐N was highly correlated with long‐term average yield (R²=0.92). For the soils evaluated, this method provided a rapid measure of potentially mineralizable N.     

限水灌溉下施氮量对冬小麦产量、氮素利用及氮平衡的影响

2008~2009年通过大田试验,研究了限水灌溉条件下,不同施氮量对冬小麦产量、氮素利用、土壤硝态氮动态变化及氮素平衡的影响。结果表明,施用氮肥显著增加小麦穗数和穗粒数,对千粒重无显著影响。作物产量、吸氮量与施氮量均呈抛物线关系,施氮量超过N240 kg/hm2,产量和吸氮量随施氮量增加略有降低。小麦起身期后,0—100 cm土层都有硝态氮分布,且随土层深度增加而减少;相同土层则随施氮量的增加而增加。土壤硝态氮积累量随生育期推进而降低,N0和N120处理分别在拔节期和开花期后表现出氮素亏缺;成熟期,土壤表观盈余以残留为主,表观损失量占小部分。氮肥表观利用率、农学利用率随施氮量增加呈降低趋势,而氮素残留率随施氮量增加呈增加趋势。在本试验条件下,施氮量在N 180~220 kg/hm2水平可以达到产量、氮素表观利用率、氮素残留率的较好结合,是限水灌溉下兼顾经济效益与环境效益的适宜施氮量。     

不同灌溉施肥方式下尿素态氮在土壤中迁移转化特性的研究

采用室内土柱模拟试验方法 ,研究了不同灌溉施肥方式下尿素态氮在土壤中的迁移、淋溶和转化特征。结果表明 ,灌水量及水肥供应方式是决定尿素态氮在土壤中迁移、转化和淋失的关键因素。氮素淋溶量随灌水量的增加而增加 ;与浇灌施肥相比 ,滴灌施肥显著地降低了氮素的淋溶损失。在淋失的氮素形态中 ,以尿素态氮为主 ,其次为硝态氮 ,铵态氮的淋失量最低。灌水量低时 ,滴灌施肥铵态氮在土壤上层明显累积 ;灌水量增加后 ,这种累积作用减弱。灌水量低时 ,灌溉施肥的土壤硝态氮变化呈上低下高 ,增加灌水量降低了土壤中硝态氮含量 ;滴灌施肥显著地减少了尿素态氮的淋溶损失 ,增加了土壤中有效态氮的含量。     

旱作地区长期小麦连作和苜蓿连作土壤剖面的矿质氮研究

对不同施肥条件下23年小麦连作地和苜蓿连作地土壤矿质氮分布和累积进行研究,探讨种植浅根系和深根系植物对硝态氮淋溶的影响。结果表明,不施肥(CK)和单施磷(P)肥,小麦和苜蓿连作地土壤硝态氮主要集中在0—60 cm土层,0—60 cm土层以下硝态氮含量变化稳定并小于2 mg/kg。氮肥、磷肥和有机肥配施(NPM)时,小麦连作地土壤硝态氮累积在20—100 cm和140—320 cm土层,年累积速率可达42.12 kg/(hm2.a);苜蓿连作土壤硝态氮主要集中在0—60 cm土层,仅在200—300 cm土层出现轻微累积,年累积速率仅为1.01 kg/(hm2.a)。在不施肥和单施磷肥下,种植小麦或苜蓿对土壤硝态氮残留量影响不显著,而氮、磷和有机肥配施时,小麦连作地土壤硝态氮残留量迅速增加,并与不施肥、单施磷肥处理有显著差异;苜蓿连作地土壤硝态氮残留量虽有少量增加,但与不施肥、单施磷肥处理无显著差异。不施肥、单施磷肥和氮、磷和有机肥配施,小麦连作、苜蓿连作地土壤剖面铵态氮含量主要在10—20 mg/kg之间波动,在土壤剖面无明显的累积现象,铵态氮残留量受施肥和作物种类的影响不显著。