稻草及其制备的生物质炭对土壤团聚体有机碳的影响

向土壤中添加生物质炭已被认为是改善土壤质量,增加碳吸存的有效措施。通过模拟实验,利用同位素δ13C标记技术,研究稻草及其制备的生物质炭添加对土壤团聚体有机碳的影响。结果表明：稻草和生物质炭对土壤团聚体中新形成碳和原有机碳的影响截然不同。培养112 d,来自稻草或生物质炭的新碳主要进入到中团聚体（50 ~ 250 μm）中,比例为70.3% ~ 75.3%。与对照土壤相比,稻草添加显著促进了大团聚体（250 ~ 2 000 μm）原有机碳的分解（p <0.05）,但对中团聚体和微团聚体（<50 μm）原有机碳的影响并不明显,而生物质炭添加（SB250和SB350）则对大团聚体和中团聚体原有机碳没有显著影响,但SB250处理（土壤中加入250℃热解制备的生物质炭）显著抑制了微团聚体原有机碳的分解（p <0.05）,而SB350处理（土壤中加入350℃热解制备的生物质炭）的则无影响。对于同一粒级团聚体,稻草与生物质炭处理的区别,主要体现在新碳分配上,而对原有机碳的影响并不显著。     

Effect of ultrasonic power on soil organic carbon fractions

Ultrasonic dispersion is a prevalent tool for soil fractionation. It is widely ignored that variation in ultrasonic power might lead to significantly different dispersion. We evaluated the effect of power variation with constant energy on the fine fraction mass, its organic C content and quality. All parameters increased significantly with power. The term “stable aggregates” as used in fractionation schemes cannot be defined by ultrasonic energy alone but power needs to be standardized, too.     

不同有机物料对苏打盐化土有机碳和活性碳组分的影响

【目的】在大同盆地苏打盐化土上,研究不同有机物料对春玉米产量、土壤有机碳及活性碳组分的影响,明确土壤有机碳及活性碳组分与主要盐碱指标的相关关系,为苏打盐化土改良及有机物料资源化利用提供理论支撑。【方法】2016-2017年在山西省北部怀仁县开展田间定位试验,设对照(CK)、风化煤、生物炭、牛粪和秸秆5个处理,各处理有机物料施用量按照每年9000 kg/hm^2等有机碳投入量折算,收获时对春玉米进行测产。2017年春玉米收获后,采集土壤样品测定土壤有机碳总量(SOC)和水溶性有机碳(WSOC)、易氧化有机碳(EOC)、轻组有机碳(LFOC)含量,分析土壤活性碳组分占有机碳的比例、土壤有机碳及活性碳组分与盐碱指标之间的关系。【结果】与CK相比,生物炭和秸秆处理春玉米产量无明显差异,而风化煤和牛粪处理春玉米产量则分别显著提高30.2%和30.3%。添加有机物料促进了0-20 cm土层SOC累积,其中以风化煤和牛粪处理效果最佳,较CK分别提高47.6%和36.1%。在有机碳组分方面,风化煤和牛粪处理提高WSOC、EOC含量的效果显著高于生物炭、秸秆处理;风化煤、牛粪和秸秆处理的LFOC含量显著高于生物炭处理。四类有机物料处理的WSOC占总有机碳的比例差异不显著,牛粪处理的占比显著高于CK。EOC占总有机碳的比例以牛粪处理最高,风化煤次之,且二者均显著高于CK处理;LFOC占总有机碳的比例则表现为秸秆、牛粪>风化煤、生物炭> CK。此外,添加有机物料能有效降低0-20 cm土层土壤pH、电导率(EC)和碱化度(ESP),其中以风化煤和牛粪处理降幅最大。相关分析表明,土壤SOC与pH、EC和ESP呈显著负相关。【结论】通过有机物料改良效果比较,发现牛粪和风化煤处理能促进苏打盐化土有机碳累积,提高可溶性、易氧化态及轻组有机碳组分在总有机碳中的占比,降低土壤pH、EC和ESP,明显提高春玉米产量。因此,风化煤和牛粪是山西北部苏打盐化土良好的改良剂。     

Tillage effect on organic carbon in a purple paddy soil

The distribution and storage of soil organic carbon （SOC） based on a long-term experiment with various tillage systems were studied in a paddy soil derived from purple soil in Chongqing, China. Organic carbon storage in the 0-20 and 0-40 cm soil layers under different tillage systems were in an order： ridge tillage with rice-rape rotation （RT-rr） 〉 conventional tillage with rice only （CT-r） 〉 ridge tillage with rice only （RT-r） 〉 conventional tillage with rice-rape rotation （CT-rr）. The RT-rr system had significantly higher levels of soil organic carbon in the 0-40 cm topsoil, while the proportion of the total remaining organic carbon in the total soil organic carbon in the 0-10 cm layer was greatest in the RT-rr system. This was the reason why the RT-rr system enhanced soil organic carbon storage. These showed that tillage system type was crucial for carbon storage. Carbon levels in soil humus and crop-yield results showed that the RT-rr system enhanced soil fertility and crop productivity. Adoption of this tillage system would be beneficial both for environmental protection and economic development.     

施肥对土壤有机碳含量及碳库管理指数的影响

在华北夏玉米生产体系中,采用田间试验,研究了不同施肥措施下（不施肥、单施有机肥、推荐施肥、习惯施肥和单施化肥）,土壤有机碳含量、活性有机碳含量和碳库管理指数的变化。结果表明:与不施肥相比,单施有机肥土壤有机碳和活性有机碳含量分别增加 11.68%,21.71%。推荐施肥和习惯施肥土壤有机碳含量分别增加 6.57%,7.58%,活性有机碳含量分别增加 8.53%,4.26%。单施化肥土壤有机碳与活性有机碳含量均没有显著增加;施有机肥和推荐施肥土壤碳库管理指数比不施肥分别高 31.79,13.01。单施化肥土壤碳库管理指数没有显著变化;土壤活性有机碳与总有机碳、碳库管理指数、玉米子粒产量均存在极显著相关关系。碳库管理指数与玉米子粒产量极显著相关,能够指示土壤生产力的变化。可见在当地土壤肥力条件下,施有机肥或有机无机适当配施能提高土壤有机碳含量和土壤碳库管理指数,有利于改善土壤质量,提高土壤肥力。     

不同地表覆盖栽培对旱地土壤有机碳、无机碳和轻质有机碳的影响

通过田间长期定位试验,分层采集冬小麦-休闲种植体系0—40 cm土层的土样,研究了常规、地表覆膜和覆草栽培对土壤有机碳、无机碳和轻质有机碳的影响。结果表明,覆膜或覆草可以显著增加地上部小麦生物量和子粒产量。不同地表覆盖对0—40 cm土层的无机碳含量和分布无显著影响,但与常规栽培相比,地表覆膜使0—5 cm土层的有机碳含量显著降低,0—40 cm各土层轻质有机碳表现出明显降低趋势,平均降低 C 6.1~74.5 mg/kg;地表覆草却表现出明显增加土壤轻质有机碳的趋势,0—5,5—10,10—20 cm土层的轻质有机碳含量分别增加C 235.2、190.0和144.9 mg/kg,相当于常规的38.7%,32.9%和34.5%。同时,覆草栽培还表现出降低0—10 cm土层轻质有机质含碳量的趋势,并使0—20 cm土层轻质有机碳占有机碳的比例显著高于常规栽培和地表覆膜处理。可见,地表长期覆膜不利于旱地土壤有机碳累积,覆草不仅可以增加表层土壤的轻质有机碳累积,还可改善土壤碳氮组成。     

Tillage effects on soil aggregation and soil organic carbon profile distribution under Mediterranean semi‐arid conditions

In rainfed semi‐arid agroecosystems, soil organic carbon (SOC) may increase with the adoption of alternative tillage systems (e.g. no‐tillage, NT). This study evaluated the effect of two tillage systems (conventional tillage, CT vs. NT) on total SOC content, SOC concentration, water stable aggregate‐size distribution and aggregate carbon concentration from 0 to 40 cm soil depth. Three tillage experiments were chosen, all located in northeast Spain and using contrasting tillage types but with different lengths of time since their establishment (20, 17, and 1‐yr). In the two fields with mouldboard ploughing as CT, NT sequestered more SOC in the 0–5 cm layer compared with CT. However, despite there being no significant differences, SOC tended to accumulate under CT compared with NT in the 20–30 and 30–40 cm depths in the AG‐17 field with 25–50% higher SOC content in CT compared with NT. Greater amounts of large and small macroaggregates under NT compared with CT were measured at 0–5 cm depth in AG‐17 and at 5–10 cm in both AG‐1 and AG‐17. Differences in macroaggregate C concentration between tillage treatments were only found in the AG‐17 field at the soil surface with 19.5 and 11.6 g C/kg macroaggregates in NT and CT, respectively. After 17 yr of experiment, CT with mouldboard ploughing resulted in a greater total SOC concentration and macroaggregate C concentration below 20 cm depth, but similar macroaggregate content compared with NT. This study emphasizes the need for adopting whole‐soil profile approaches when studying the suitability of NT versus CT for SOC sequestration and CO₂ offsetting.     

不同秸秆还田模式对土壤有机碳及其活性组分的影响

为了探讨不同秸秆还田模式对土壤有机碳(total organic carbon,TOC)及活性碳组分的影响,设置了秸秆不还田(CK)、秸秆直接还田(CS)、秸秆转化为食用菌基质,出蘑后菌渣还田(CMS)和秸秆过腹还田(CGS)4种还田模式。通过田间小区试验,研究了不同秸秆还田模式下,土壤有机碳及活性组分的变化规律。结果表明不同秸秆还田模式均提高了土壤有机碳含量,但不同还田模式下土壤有机碳含量差异不显著(P0.05),和CK相比,CS、CMS和CGS处理下,土壤有机碳质量分数分别增加9.0%、23.9%和26.7%。不同秸秆还田模式也提高了土壤活性碳组分含量。在不同秸秆还田模式下,土壤溶解性有机碳(dissolved organic carbon,DOC)含量表现为CSCMSCGSCK,且不同处理间差异显著(P0.01)。和CK相比,CS、CMS和CGS处理下,土壤DOC质量分数分别增加64.6%、29.4%和8.9%。土壤微生物量碳(microbial biomass carbon,MBC)含量表现为CMSCGSCSCK,且差异显著(P0.05)。和CK相比,CS、CMS和CGS处理下,土壤MBC质量分数分别增加28.9%、84.7%和59.3%。土壤易氧化态碳(easily oxidizable carbon,EOC)含量表现为CMSCSCGSCK,且差异显著(P0.01)。和CK相比,CS、CMS和CGS处理下,土壤EOC质量分数分别增加24.1%、55.7%、和9.3%。不同秸秆还田模式显著影响土壤活性碳组分在总有机碳中占的比例,改变土壤有机碳质量。在不同秸秆还田模式下,DOC/TOC表现为CSCMSCKCGS、MBC/TOC表现为CMSCGSCSCK、EOC/TOC表现为CMSCSCKCGS,且不同处理间均差异显著(P0.01)。从提高土壤质量角度,推荐秸秆-菌渣还田模式,在该模式下,土壤MBC/TOC和EOC/TOC均最大,土壤碳素有效性高、易于被微生物利用,有利于作物生长。从提高土壤固碳角度,推荐秸秆过腹还田模式,在该模式下,土壤DOC/TOC最小,且土壤有机碳含量最高,有利于碳的固定和保存。该研究结果可为秸秆合理高效利用、改善农业土壤碳库质量提供参考。     

Effects of nitrogen fertilization on pot‐grown wheat photosynthate partitioning within intensively farmed soil determined by 13C pulse‐labeling

Understanding rhizodeposited carbon (C) dynamics of winter wheat (Triticum aestivum L.) is important for improving soil fertility and increasing soil C stocks. However, the effects of nitrogen (N) fertilization on photosynthate C allocation to rhizodeposition of wheat grown in an intensively farmed alkaline soil remain elusive. In this study, pot‐grown winter wheat under N fertilization of 250 kg N ha^?1 was pulse‐labeled with ¹³CO₂ at tillering, elongation, anthesis, and grain‐filling stages. The ¹³C in shoots, roots, soil organic carbon (SOC), and rhizosphere‐respired CO₂ was measured 28 d after each ¹³C labeling. The proportion of net‐photosynthesized ¹³C recovered (shoots + roots + soil + soil respired CO₂) in the shoots increased from 58–64% at the tillering to 86–91% at the grain‐filling stage. Likewise, the proportion in the roots decreased from 21–28% to 2–3%, and that in the SOC pool increased from 1–2% to 6–7%. However, the ¹³C respired CO₂ allocated to soil peaked (17–18%) at the elongation stage and decreased to 6–8% at the grain‐filling stage. Over the entire growth season of wheat, N fertilization decreased the proportion of net photosynthate C translocated to the below‐ground pool by about 20%, but increased the total amount of fixed photosynthate C, and therefore increased the below‐ground photosynthate C input. We found that the chase period of about 4 weeks is sufficient to accurately monitor the recovery of ¹³C after pulse labeling in a wheat–soil system. We conclude that N fertilization increased the deposition of photoassimilate C into SOC pools over the entire growth season of wheat compared to the control treatment.     

土壤有机碳研究进展

回顾了国内外土壤有机碳研究进展及趋势,阐述了全球土壤有机碳库存量及分布、我国土壤有机碳库储量概况、农田土壤有机碳组成及其影响因素、农田土壤有机碳转化规律及影响因素,指出了我国在土壤有机碳研究方面存在的问题及今后的发展方向.     

Near‐infrared spectroscopy can predict the composition of organic matter in soil and litter

The usefulness and limitations of near‐infrared reflectance spectroscopy (NIRS) for the assessment of several soil characteristics are still not sufficiently explored. The objective of this study was to evaluate the ability of visible and near‐infrared reflectance (VIS‐NIR) spectroscopy to predict the composition of organic matter in soils and litter. Reflectance spectra of the VIS‐NIR region (400–2500 nm) were recorded for 56 soil and litter samples from agricultural and forest sites. Spectra were used to predict general and biological characteristics of the samples as well as the C composition which was measured by ¹³C‐CPMAS‐NMR spectroscopy. A modified partial least‐square method and cross‐validation were used to develop equations for the different constituents over the whole spectrum (1st to 3rd derivation). Near‐infrared spectroscopy predicted well the C : N ratios, the percentages of O‐alkyl C and alkyl C, the ratio of alkyl C to O‐alkyl C, and the sum of phenolic oxidation products: the ratios of standard deviation of the laboratory results to standard error of cross‐validation (RSC) were greater than 2, the regression coefficients (a) of a linear regression (measured against predicted values) ranged from 0.9 to 1.1, and the correlation coefficients (r) were greater than 0.9. Satisfactorily (0.8 ≤ a ≤ 1.2, r ≥ 0.8, and 1.4 ≤ RSC ≤ 2.0) assessed were the contents of C, N, and production of DOC, the percentages of carbonyl C and aromatic C and the ratio of alkyl C to aromatic C. However, the N‐mineralization rate and the microbial biomass were predicted unsatisfactorily (RSC < 1.4). The good and satisfactory predictions reported above indicate a marked usefulness of NIRS in the assessment of biological and chemical characteristics of soils and litter.     

Impacts of land‐use intensity on soil organic carbon content,soil structure and water‐holding capacity

The impact of land‐use intensity is evaluated through changes in the soil properties in different areas of the traditional central Spanish landscape. Soil organic carbon (SOC) content, bulk density, aggregate stability and water‐holding capacity (WHC) in the topsoil of active and abandoned vineyards, livestock routes (LR) and young Quercus afforested areas were analysed. These different types of land use can be interpreted as having a gradient of progressively less impact on soil functions or conservation. As soil use intensity declines, there is an increase in SOC content (from 0.2 to 0.6%), WHC (from 0.2 to 0.3 g H₂O per g soil) and aggregate stability (from 4 to 33 drop impacts). Soils beneath vines have lost their upper horizon (15 cm depth) because of centuries‐old tillage management of vineyards. Except for an increase in bulk density (from 1.2 to 1.4 g/cm³), there were no differences in soil characteristics 4 yr after the abandonment of vine management. LR can be considered sustainable uses of land, which preserve or improve soil characteristics, as there were no significant differences between topsoil from LR and that from a 40‐yr‐old Quercus afforested area. SOC content, one of the main indicators for soil conservation, is considered very low in every case analysed, even in the more conservative uses of land. These data can be useful in understanding the slow rate of recovery of soils, even after long‐term cessation of agricultural land use.     

Natural 13C abundance and soil carbon dynamics under long‐term residue retention in a no‐till maize system

Residue retention and reduced tillage are both conservation agricultural practices that may enhance soil organic carbon (SOC) stabilization in soil. We evaluated the long‐term effects of no‐till (NT) and stover retention from maize on SOC dynamics in a Rayne silt loam Typic Hapludults in Ohio. The six treatments consisted of retaining 0, 25, 50, 75, 100 and 200% of maize residues on each 3 × 3 m plot from the crop of previous year. Soil samples were obtained after 9 yrs of establishing the experiment. The whole soil (0–10 and 10–20 cm of soil depths) samples under different treatments were analysed for total C, total N, recalcitrant C (NaOCl treated sample) and ¹³C isotopic abundance (0–10 cm soil depth). Complete removal of stover for a period of 9 yrs significantly (P < 0.01) decreased soil C content (15.5 g/kg), whereas 200% of stover retention had the maximum soil C concentration (23.1 g/kg). Relative distribution of C for all the treatments in different fractions comprised of 55–58% as labile and 42–45% as recalcitrant. Retention of residue did not significantly affect total C and N concentration in 10–20 cm depth. ¹³C isotopic signature data indicated that C₄‐C (maize‐derived C) was the dominant fraction of C in the top 0–10 cm of soil layer under NT with maize‐derived C accounting for as high as 80% of the total SOC concentration. Contribution of C₄‐C or maize‐derived C was 71–84% in recalcitrant fraction in different residue retained plots. Residue management is imperative to increase SOC concentrations and long‐term agro‐ecosystem necessitates residue retention for stabilizing C in light‐textured soils.     

Long‐term no‐tillage effects on particulate and mineral‐associated soil organic matter under rainfed Mediterranean conditions

Soil organic carbon (SOC) plays an essential role in the sustainability of natural and agricultural systems. The identification of sensitive SOC fractions can be crucial for an understanding of SOC dynamics and stabilization. The objective of this study was to assess the effect of long‐term no‐tillage (NT) on SOC content and its distribution between particulate organic matter (POM) and mineral‐associated organic matter (Min) fractions in five different cereal production areas of Aragon (north‐east Spain). The study was conducted under on‐farm conditions where pairs of adjacent fields under NT and conventional tillage (CT) were compared. An undisturbed soil nearby under native vegetation (NAT) was included. The results indicate that SOC was significantly affected by tillage in the first 5 cm with the greatest concentrations found in NT (1.5–43% more than in CT). Below 40 cm, SOC under NT decreased (20–40%) to values similar or less than those under CT. However, the stratification ratio (SR) never reached the threshold value of 2. The POM‐C fraction, disproportionate to its small contribution to total SOC (10–30%), was greatly affected by soil management. The pronounced stratification in this fraction (SR>2 in NT) and its usefulness for differentiating the study sites in terms of response to NT make POM‐C a good indicator of changes in soil management under the study conditions. Results from this on‐farm study indicate that NT can be recommended as an alternative strategy to increase organic carbon at the soil surface in the cereal production areas of Aragon and in other analogous areas.     

温度与土壤水分对有机碳分解速率的影响

试验研究温度与土壤水分对有机碳分解速率的影响,结果表明:相同水分条件下,培育初期(1～20d)各处理CO2排放速率较高,相对值表现为35℃处理>25℃处理>15℃处理>5℃处理;随培育时间延长(>20d),CO2排放速率渐趋平稳。相同温度条件下,30%～90%田间持水量时培育初期(1～20d)各处理CO2排放速率初始值较高,之后降低,当趋于某一定值时,相对值大小随土壤水分含量增加而增加。相同温度与土壤水分条件下,CO2排放速率相对值大小随土壤有机碳含量增加而增加。     

耕作方式对华北农田土壤固碳效应的影响

研究不同耕作方式对华北农田土壤固碳及碳库管理指数的影响,可为探寻有利于农田固碳的耕作方式提供科学依据。该研究在中国农业大学吴桥实验站进行,试验于2008年设置了免耕秸秆不还田(NT0)、翻耕秸秆不还田(CT0)、免耕秸秆还田(NT)、翻耕秸秆还田(CT)和旋耕秸秆还田(RT)5个处理。研究测定分析了土壤容重、有机碳、易氧化有机碳含量及不同耕作方式下的碳库管理指数。通过对不同耕作方式下0～110cm土壤的分析,结果表明,随着土层的加深,土壤有机碳含量不断下降,NT显著增加了表层(0～10cm)土壤有机碳含量,而>10～50cm有机碳含量较其他处理(NT0除外)有所下降,深层(>50～110cm)处理间差异不明显;土壤容重与有机碳含量呈显著的负相关关系(P<0.01);0～30cm土层有机碳储量以NT最高,CT与其无明显差异,二者较CT0分别高出13.1%和11.0%,而至0～50cm土层,CT的碳储量最高,但与NT无显著差异(P<0.05);与CT0相比,NT0降低了各层土壤易氧化有机碳含量,而NT则在0～10cm土层表现为增加;RT、CT分别显著增加了0～10、>10～30cm土层的碳库管理指数。结果表明,秸秆还田可改善土壤质量,提高农田碳库管理指数,同时碳库管理指数受耕作方式的影响也较大,尤其是CT和RT;NT通过减少土壤扰动、增加有机质的输入,可提高上层土壤有机碳的储量。     

长期不同施肥下黑土与灰漠土有机碳储量的变化

采用长期试验,研究了20年不同施肥下1 m深黑土与灰漠土有机碳含量与碳储量的剖面变化。结果表明,单施化肥和不施肥对黑土1 m土层有机碳储量没有显著影响,但灰漠土略有降低。有机肥配施化肥能显著提高土壤有机碳含量和储量。高量有机肥配施化肥（NPKM2）能提高020 cm和2040 cm土层土壤有机碳含量,黑土分别提高56.6%和49.6%、灰漠土提高143.1%和46.9%;常量有机无机配施（NPKM）效果较差,增幅分别为黑土35.1%和35.3%,灰漠土80.2%和4.1%。两种土壤1 m土体的有机碳储量,NPKM2处理分别提高了C 30.7 t/hm2与C 40.6 t/hm2。显然,有机无机肥配施可以显著提高1 m深土体中有机碳储量,主要是由于提高了040 cm土层土壤有机碳含量。     

有机物料碳和土壤有机碳对水稻土甲烷排放的影响

基于30年水稻土长期施肥定位试验,在保证原有定位试验正常开展的前提下,将部分化肥处理变更为有机肥处理(或反之),通过观测一年水稻轮作周期内不同处理甲烷(CH_4)排放通量季节性变化,探讨不同肥力水稻土中外源有机碳及土壤有机碳含量对田间CH_4排放的影响。结果表明:施化肥处理和有机肥处理,水稻土全年CH_4累积排放量范围分别为1.73~4.72和35.09~86.60 g·m~(-2)。有机肥处理改施化肥后,田间土壤CH_4的排放量显著降低;化肥处理改施有机肥或有机肥处理增施有机肥后,田间土壤CH_4的排放量显著提高。外源有机碳的输入量是田间土壤CH_4年排放量的决定性因素,外源有机碳输入量(x)与水稻土CH_4年累积排放量(y)之间满足直线方程:y=0.087 7 x+3.265 7(R~2=0.965 9,n=21)。土壤有机碳同样也是影响稻田CH_4排放的因素,在不同有机碳水平的水稻土上施用等量相同化肥或有机肥,土壤有机碳含量高的水稻土都更有利于CH_4的产生。单施化肥稻田土壤CH_4排放的最主要碳源是土壤有机碳,有机碳含量(x)和水稻土CH_4年累积排放量(y)之间的指数方程:y=0.162 4 e~(0.162 2 x)(R~2=0.940 6,n=9)。有机肥可促进土壤有机碳分解释放CH_4,土壤有机碳含量相同的条件下,高量有机肥比常量有机肥的土壤有机碳分解比率高0.65%,等量相同有机肥但土壤有机碳含量不同的条件下,土壤有机碳分解比率无显著差异;同样,土壤有机碳也可促进有机物料碳分解释放CH_4,在常量有机肥或高量有机肥处理中,土壤有机碳含量高者比低者的有机物料碳分解比率分别多出3.57%和2.34%。