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1.
利用猪粪液为菌源体外发酵产气法评价牧草纤维品质   总被引：3，自引：0，他引：3  
利用体外产气法比较杂交狼尾草、优质矮化象草、柱花草的纤维品质,为牧草在猪饲养中的应用提供依据。提取牧草中的中性洗涤纤维作底物,以新鲜猪粪作为发酵菌源,39℃恒温摇床中培养24 h,测定24 h内不同时段的产气量、发酵液pH值和VFA。研究结果表明,中性洗涤纤维的含量为柱花草>杂交狼尾草>优质矮化象草,分别是69.02,61.27和54.38 g/100 g。发酵24 h,3种牧草的累积产气量杂交狼尾草最高,优质矮化象草次之,柱花草最低,分别为137.62,112.66和79.62 mL/g DM;发酵6 h以前,3种牧草的累积产气量仅占24 h平均累积产气量的7.58%。理论最大产气量杂交狼尾草与优质矮化象草均高于柱花草,分别为172.13,166.57和78.27 mL。3种牧草每克有机物发酵产生1 mL气体的持续时间表现为,柱花草<杂交狼尾草<优质矮化象草,分别为2.89,4.26和5.02 h。3种牧草达到最大产气速率的时间以优质矮化象草最高、杂交狼尾草次之、柱花草最低,分别为20.34,18.09和13.20 h。3种牧草的产气速度表现为,杂交狼尾草>优质矮化象草>柱花草,分别为10.11,8.31和6.74mL/h。研究表明,不同底物发酵24 h产生的挥发性脂肪酸中,3种酸的浓度比例发生了变化,并主要由丁酸的比例变化所引起;不同科以及同科中的不同属牧草具有不同的发酵动力学特征,主要因牧草中NDF的组成和单体的化学结构不同所致。研究给出了3种牧草体外发酵产气模型回归方程,系统分析了评定中性洗涤纤维品质的适宜指标。  相似文献   

2.
    
Cannabis sativa L. is an annual herbaceous plant. It was used for centuries to obtain different products. In the last century, hemp cultivation was forbidden due to the psychoactive effects of tetrahydrocannabinol acid (THCA). In the last years, new strains, characterized by high cannabidiolic acid (CBDA) and low THCA level, were developed renewing the interest in hemp cultivation to obtain food or to extract essential oils from flowers. All these processes produce many residues with different chemical–physical characteristics. In order to evaluate their potential use also in animal nutrition, some hemp co-products were evaluated. Two different co-products of seed processes (flour and oil) and two co-products obtained trimming the flowers, differing in granulometry were used. The samples were analysed for chemical composition and evaluated in vitro using the gas production technique with buffaloes' ruminal inoculum. All hemp co-products showed interesting nutritional characteristics, such as crude protein content always higher than 20% on a dry matter basis, and high neutral detergent fibre concentration partially lignified. The in vitro gas production parameters at 120 h of incubation showed quite low fermentability testified by the low organic matter degradability and cumulative gas volume (OMD from 28.09 to 45.64% and OMCV from 110 to 164 ml/g, respectively). Also, the methane produced after 24 h of incubation was particularly low (from 1.78 to 11.73 ml/g dOM). These results could be due to the high lipid and ash amounts or to the CBDA content that probably affected the CH4 formation processes. According to preliminary results obtained by this study, it is possible to hypothesize that these co-products could be useful to mitigate the methane production into the rumen. Further studies are necessary in order to evaluate the correct inclusion into the diet for ruminants.  相似文献   

3.
本试验旨在利用体外产气法研究3个品种柱花草与王草间的组合效应。将3个品种柱花草[热研2号、20号、21号柱花草]与热研4号王草分别以6个比例[0∶100、10∶90、20∶80、40∶60、50∶50、100∶0]组合,利用体外产气技术,分析不同比例的组合对产气量、产气参数、体外干物质消化率(IVDMD)、氨态氮(NH3-N)和微生物蛋白(M CP)含量的影响,计算各组合的单项组合效应值和综合组合效应值,进而筛选出各品种柱花草与王草的适宜比例。结果表明:1)热研2号和20号柱花草与王草不同比例组合的快速发酵部分产气量差异达到显著水平(P0.05),快速发酵部分产气量达到最大的组合比例分别为30∶70和50∶50。热研2号柱花草∶王草、热研21号柱花草∶王草分别为50∶50、40∶60时的IVDMD最高,与仅有王草做底物时差异显著(P0.05)。热研2号柱花草∶王草、热研20号柱花草∶王草为30∶70时MCP含量最高。NH3-N含量随着柱花草比例的递增出现先增高后降低的趋势,各品种柱花草与王草组合均以30∶70时最高。2)3个品种柱花草与王草的综合组合效应值均随着柱花草比例的增大而呈现单峰型增长趋势,其中热研2号柱花草∶王草和热研20号柱花草∶王草为30∶70时最高,热研21号柱花草∶王草为20∶80时最高。综合得出,在王草中添加适宜比例的柱花草能够提高体外发酵的产气量、IVDMD和MCP含量,提高瘤胃微生物活力,产生正组合效应;热研2号、20号柱花草与王草均以30∶70组合效果最佳,热研21号柱花草与王草以20∶80组合效果最佳。  相似文献   

4.
    
This study aimed to investigate the effect of grape pomace powder levels and roughage sources on gas kinetics, digestibility and fermentation of swamp buffaloes by using in vitro techniques. The experimental design was a 2 × 4 factorial arrangement in a completely randomized design. Factor A was two sources of roughage (untreated rice straw, RS, and 3% urea treated rice straw, UTRS) and factor B was four levels of grape pomace powder (GPP) supplementation (0, 2, 4, 6% of substrate) on a dry matter basis. Results revealed that GPP supplementation at 2, 4 and 6% of substrate influenced gas kinetics. Cumulative gas production tended to be lower in the supplemented group. In vitro true digestibility was higher in the GPP supplementation at 2% with UTRS while microbial mass was higher in the supplemented groups. Supplementation of GPP significantly increased the total volatile fatty acids, especially propionate. Calculated methane production was subsequently decreased in the supplemented groups. Bacterial population was higher while protozoal population was lower by GPP supplementation. It could be concluded that supplementation of GPP at 2% of the substrate with UTRS improved in vitro true digestibility, rumen fermentation end‐products as well as reducing methane production.  相似文献   

5.
旨在探讨利用体外产气法研究不同瘤胃降解蛋白平衡日粮(RDPB,g·kg-1 DM)对瘤胃发酵、总产气量和甲烷产量的影响.试验选用美国Ankom产气系统,采用随机区组试验设计,配置6种不同瘤胃降解蛋白平衡日粮,分别为日粮1(-20 g· kg-1 DM),日粮2(-10 g· kg-1 DM),日粮3(0 g· kg-1 DM),日粮4(10g·kg-1DM),日粮5(20 g·kg-1 DM)和日粮6(30 g· kg-1 DM),每组2个重复,培养期为24 h.结果表明:(1)本研究中6种类型日粮发酵液的pH、乙酸/丙酸、产气量和CH4产生量表现为日粮3(P＜0.01)或日粮4(P＜0.01)值最低.(2)挥发性脂防酸乙酸、丙酸、丁酸以及氨氮的浓度随着瘤冒降解平衡值的增加而增加,表明改变日粮瘤胃降解蛋白平衡可以影响瘤胃发酵.而瘤胃降解蛋白平衡对总挥发性脂肪酸以及原虫数量无显著影响.(3)日粮瘤胃降解蛋白平衡值影响不同时间点甲烷的浓度和24 h甲烷产量.其中以日稂4(P＜0.05)甲烷总产量最低.由此可见,在体外培养条件下,改变日粮RDPB影响了瘤胃发酵液pH、氨态氮(NH3-N)及挥发性脂肪酸(VFA)浓度、总产气量、CH4产生量,综合考虑其对瘤胃发酵和甲烷产量等指标的影响,体外产气条件下适宜的RDPB水平为0～10g·kg-1 DM.  相似文献   

6.
为了评定不同生育期虉草的营养价值,分析体外产气法和CNCPS法两种营养评价方法的相关性。采用体外产气法测定抽穗期、开花期、灌浆期、乳熟期4个生育期虉草的体外发酵产气量、发酵参数(a、b、c)、DM消化率、NDF和ADF降解率,用康奈尔净碳水化合物和蛋白质体系(cornell net carbohydrate and protein system,CNCPS)分析方法测定相应组分,评价其营养价值,并比较研究两种方法评价结果的相关性。结果表明,体外发酵至72 h结束,累计总产气量和产气参数a以开花期最高(122.68 和4.46 mL),显著高于各组(P<0.05),且从抽穗期、灌浆期、乳熟期有降低的趋势;抽穗期和开花期的DM消化率、NDF和ADF降解率分别显著高于灌浆期和乳熟期(P<0.05)。随着虉草生育期的延长,CP和SOLP呈递减趋势,即抽穗期和开花期显著高于灌浆期和乳熟期(P<0.05);NDF、ADF和ADL呈递增趋势,但NDFIP、ADFIP呈递减趋势。灌浆期和乳熟期的CHO含量显著高于抽穗期和开花期(P<0.05),而CA和CNSC含量则相反,且CC含量同样以灌浆期与乳熟期较高;蛋白质组分中,PA与CA及CC与PC组分呈现同样的趋势。虉草CNSC、CA、CB1、CB2、PA、PB3组分含量与不同时间点产气量呈显著或极显著的相关性(P<0.05或P<0.01)。因此,开花期虉草营养价值最高,其次为抽穗期、灌浆期和乳熟期,产气法和CNCPS评价牧草具有相关性,用体外产气量估测牧草CNCPS组分是可行的。  相似文献   

7.
    
The objective of the study was to evaluate effects of Quillaja saponaria (QS) with or without Yucca schidigera extract (YS) on in vitro ruminal fermentation and methanogenesis. The culture media consisted of 400 mL of strained rumen fluid collected from two non‐lactating Holstein cows and 400 mL of artificial saliva. The culture media was anaerobically incubated with 10 g of a mixture of concentrate and oat hay (1:1, w/w) at 39°C for 24 h. The treatments were arranged as 2 × 3 factorial design experiment consisting of three levels of QS (0, 2 or 4 mL/L) and two levels of YS (0 or 2 mL/L). Treatment interactions between QS and YS were observed for pH and ammonia N concentration. CH4 production was not modified by addition of QS but decreased (P < 0.05) when added with YS. Addition of QS with YS increased (P < 0.05) propionate concentrations. Protozoa numbers were decreased (P < 0.05) by QS addition alone or with YS. Results show that QS and YS exhibited strong antiprotozoal effects and combinations of both plant extracts may have potential as safe manipulators of ruminal fermentation.  相似文献   

8.
选取3种典型的植物油:菜籽油、豆油和亚麻油,利用体外培养系统进行体外发酵,研究植物油的类型和添加水平对瘤胃体外发酵和甲烷生成的影响。3种植物油分别添加底物干物质的3%、5%和7%,未添加植物油组为对照,共10个处理,每个处理设3个重复。结果表明,菜籽油对甲烷产量无显著影响(P>0.05),豆油7%添加水平显著抑制了甲烷生成,而亚麻油所有添加水平均显著降低了甲烷生成量(P<0.05)。在豆油和亚麻油处理组中,随着添加水平的提高,甲烷产量线性下降(P<0.01)。3种植物油均显著改变了瘤胃发酵模式,随着植物油中所含不饱和脂肪酸不饱和度的增加,乙酸和丁酸的摩尔比例显著降低,丙酸摩尔比例显著升高(P<0.05);植物油添加水平的提高显著降低了乙酸和丁酸摩尔比例,显著升高了丙酸摩尔比例(P<0.05),乙酸丙酸比例显著降低。本试验结果表明植物油降低甲烷的效果跟其来源和添加水平有关;随着植物油添加水平的提高和所含不饱和脂肪酸不饱和度的增加,甲烷生成量显著降低。  相似文献   

9.
Chemical composition, in vitro digestibility and gas production of straw from four different varieties of Kabuli chickpea (Cicer arietinum) were studied. Kinetics of fermentation of straws from 19 different accessions of chickpea was also evaluated using gas production technique. Significant difference (p < 0.05) was observed in the yield of straw [from 1041 to 1174 kg dry matter (DM)/ha] from different varieties. The proportion of seed/straw from different varieties varied from 0.61 to 0.93. Crude protein, neutral detergent fibre and acid detergent fibre content of straw ranged from 28.1 to 35.8, 598.6 to 645.4 and 459.2 to 473.4 g/kg DM respectively. Organic matter digestibility was different (p < 0.05) among varieties and varied from 471.4 to 535.5 g/kg DM. Potential gas production (A), the rate constants (c and d) and lag times of straws from different chickpea varieties were not different (p > 0.05). However, the rate constants (c and d) and lag times were different (p < 0.05) among accessions. Potential gas production (A) differed (p < 0.05) approximately twofold among different accessions. The results emphasized that in any evaluation of chickpea varieties or accessions, where straw of this legume seed is used as an animal feed, not only seed yield but also yield and quality of straw should be taken into consideration.  相似文献   

10.
不同牧草品种对体外发酵产气特性的影响   总被引：8，自引：6，他引：8  
采用改进的体外产气技术评价了10种不同牧草的体外产气特性.利用非线性回归方程获得了Gompertz模型的发酵动力学参数(理论最大产气量、产气速率常数和延滞时间),分析了不同品种牧草间发酵动力学参数的差异及发酵动力学参数与营养成分之间的相关关系.结果表明,不同品种牧草间产气动力学参数差异显著(P<0.05).理论最大产气量、产气速率常数和产气延滞时间分别以苏丹草、黑麦草和桂牧1号最高,而以鲁梅克斯、鲁梅克斯和苏丹草最低.相关分析表明理论最大产气量和累积产气量分别与OM含量、NDS/CP值及(NDS-Ash)/CP值呈极显著正相关关系(P<0.001),而与CP含量呈极显著负相关关系(P<0.01).产气速率常数与营养成分无显著相关性.产气延滞时间与半纤维素的含量呈极显著正相关关系(P<0.01),而与NDS/CP值和(NDS-Ash)/CP值呈极显著负相关关系(P<0.001).结论认为牧草中非结构性碳水化合物与蛋白质比例决定了体外发酵产气的特性.  相似文献   

11.
本研究旨在探讨添加无患子皂甙(SAD)对瘤胃发酵及甲烷产量的影响。通过体外培养法,以玉米粉、羊草粉(50∶50)为底物,设置对照组(无添加)、添加底物量1.5%的茶皂素组(TS,正对照)和SAD(与TS相同皂甙量),测定培养12、24 h后各组产气量和甲烷产量,以及24 h培养液的pH值、挥发性脂肪酸浓度、氨态氮浓度和原虫数。结果表明:在12 h和24 h时,SAD组产气量均提高(P<0.01和P<0.05)。12 h时,SAD和TS组甲烷产量均显著降低(P<0.05),24 h时达到极显著(P<0.01)。SAD和TS组总挥发性脂肪酸浓度提高(P<0.01和P<0.05),SAD组乙酸和丙酸浓度增加(P<0.05和P<0.01),TS组丙酸浓度提高(P<0.05)。SAD组氨态氮浓度及培养液中原虫数极显著降低(P<0.01),TS组显著降低(P<0.05)。上述结果表明,添加SAD可促进瘤胃发酵,抑制原虫生长,减少甲烷产生。  相似文献   

12.
    
The objective of this study was the characterization of the nutritive value, ruminal fermentation and nutrient digestibility of field pea (Pisum sativum L.) hay (FPH) by‐product through in situ, in vitro and in vivo tests. A FPH diet was administered ad libitum to four sheep (live weight (LW) 38.0 ± 2.0 kg) at four inclusion levels (0, 25, 50 and 75%) over a conventional sorghum grain/oat straw ration in a 4 × 4 Latin square design. The in situ degradability of FPH was 67% dry matter (DM), 71% crude protein and 66% neutral detergent fiber (NDF). The inclusion of FPH did not affect (P ≥ 0.05) the DM (61 ± 0.4 g/LW0.75/day) and organic matter (OM) intake (56 ± 0.7 g/LW0.75/day). Total DM digestibility (68 ± 2.5%), OM (70 ± 3%), and NDF (60 ± 3%), were not different (P ≥ 0.05) between control and FPH inclusion diets. The diet had no effect on ruminal pH, ruminal ammonia concentration, total and individual volatile fatty acids. The chemical composition and sheep intake behavior indicate that FPH by‐product is medium quality forage when incorporated in maintenance rations.  相似文献   

13.
    
To effectively use local available grass resources to cover the winter feed shortage on the Qinghai‐Tibetan Plateau, direct‐cut and wilted reed canary grass (RCG) silages were prepared by using a rolled‐bale system, and their ensiling characteristics and in vitro digestibility were studied. Silages were treated without (control) or with inoculants including LP (Lactobacillus plantarum), LPLB (L. plantarum, L. buchneri), and LPLBc (L. plantarum, L. buchneri, and cellulase), and were stored at ambient temperature (5.7–14.6°C) for 90 days. Compared with control, the inoculated silages increased (p < .05) lactic acid and acetic acid contents, and reduced (p < .05) final pH value and ammonia‐N ratio of total N. The highest WSC content (41.2 g/kg DM) occurred for LPLB‐inoculated silage, whereas LPLBc‐treated silage displayed the lowest contents of NDF (522.9 g/kg DM) and ADF (275.5 g/kg DM). In addition, LPLBc‐inoculated silage had the highest in vitro gas production (51.0 ml/g DM), in vitro DM digestibility (619.3 g/kg DM), and metabolic energy (9.6 kJ/kg DM). These results confirmed that treatments with inoculants at ensiling could improve silage fermentation and in vitro digestibility of RCG, and this could be a potential winter feed for animals on the Qinghai‐Tibetan Plateau.  相似文献   

14.
本研究采用体外产气量法,以全日制混合日粮（TMR,精粗比60∶40）为发酵底物,比较了两种典型酵母制剂活性干酵母（active dry yeast,ADY）和酵母培养物（yeast culture,YC）对体外产气量、产气动力学参数、甲烷（CH4）含量及瘤胃发酵参数的影响,同时根据24 h产气量预测了酵母制剂对底物代谢能（ME）、有机物消化率（OMD）及微生物蛋白（MP）产量的影响,旨在比较二者在体外瘤胃发酵影响上的差异,为进一步揭示其应用效果的差异机制及其合理应用奠定基础。结果显示,ADY及YC组产气量相近,但YC组12、24、48 h的产气量均高于ADY组;ADY组发酵前期的产气速率较对照组降低了15.3%（P>0.05）,YC组较对照组升高了19.4%（P>0.05）,YC组较ADY组升高了41.0%（P<0.05）;与对照组相比,YC显著增加了CH4气体含量（P<0.05）,显著降低了H2及CO2的含量（P<0.05）。ADY显著降低了H2含量（P<0.05）,但对CH4及CO2气体无显著影响（P>0.05）;与对照组相比,ADY和YC对发酵液的pH、总挥发性脂肪酸均无显著影响（P>0.05）,但均显著升高了发酵液中氨态氮（NH3-N）的浓度（P<0.05）。ADY对个别挥发性脂肪酸比例无显著影响（P>0.05）,YC有升高乙酸比例、降低异丁酸比例的趋势;与对照组相比,ADY及YC对预测的底物OMD、ME及MP产量无显著影响（P>0.05）,但YC组各预测指标均显著高于ADY组（P<0.05）。综上所述,YC与ADY在对发酵底物的体外瘤胃产气量、气体成分含量、产气速率及发酵参数的影响上存在差异。  相似文献   

15.
    
This study was conducted to compare the effect of active dry yeast（ADY)and yeast culture（YC）on gas production,dynamics of gas production,methane content and rumen fermentation parameters by in vitro gas production technique,and the values of ME,OMD and yield of MP were also predicted according to 24 h gas production,in order to further reveal the mechanism of the difference effect and lay a foundation for those reasonable application.The results showed that ADY and YC had similiar effect on gas production, however,the gas production of YC group at 12,24 and 48 h were higher than that of ADY;Compared to CON group,the rate of gas production in earlier fermentation period was increased by 19.4% in YC group and decreased by 15.3% in ADY group（P>0.05),and that of YC group was significantly increased by 41.0% than that of ADY group（P<0.05);The content of CH4 of YC group was significantly increased and content of H2 and CO2 were significantly decreased（P<0.05).The content of H2 in ADY group was significantly decreased（P<0.05),while the content of CH4 and CO2 were not significantly changed（P>0.05);The pH and total VFA of rumen fermentation fluid in ADY and YC groups were not significantly changed（P>0.05),while the concentration of NH3-N of the two groups was significantly increased（P<0.05).ADY had no significant effect on individual VFA（P>0.05),whereas YC tended to increase the ratio of acetic acid and decrease the ratio of isobutyric acid;Comparing to CON group,the OMD,ME and MP of ADY and YC groups were not significantly affected（P>0.05), while that of YC group were all significantly increased than ADY group（P<0.05).In conclusion,there were certain differences in the effect of ADY and YC on gas production,concentrate of gas,the rate of gas production and parameter of rumen fermentation.  相似文献   

16.
试验通过评定菜籽粕、玉米、米糠、苜蓿、玉米青贮和玉米秸秆的营养价值来比较瘤胃尼龙袋法和体外产气实验。结果表明:各种饲料的干物质有效降解率分别为52.90%、54.27%、65.70%、40.76%、37.24%和38.17%,精饲料干物质有效降解率(EDMD)中,a部分占比例较高(其中米糠达到64.51%);每克饲料DM的渐进产气量(A)分别为105.00、180.51、109.30、172.71、147.76和148.66 mL,粗饲料的产气量普遍高于精饲料;两种方法中,产气参数A、B与EDMD均呈负相关(r=-0.55和r=-0.61)。从速率曲线来看,降解速率曲线主要反映了b部分的降解情况,而饲料产气的速率曲线却能明显反映出两相的发酵情况。  相似文献   

17.
不同品种牧草间组合时体外产气发酵特性研究   总被引：4，自引：1，他引：4  
采用体外发酵产气技术研究了鲁梅克斯杂交酸模-三叶草分别与皇竹草、桂牧1号、冬牧70、矮象草、牛鞭草、黑麦草和苏丹草按0∶100、25∶75、50∶50、75∶25和100∶0的比例进行组合时的体外发酵产气特性。结果表明,当鲁梅克斯-三叶草分别与皇竹、桂牧1号和冬牧70组合时,产气量和理论最大产气量以鲁梅克斯-三叶草占75%时最高,而与黑麦草组合时则以25%时最高,并显著(P<0.05)高于其占75%的组合。与矮象草和苏丹草组合时以其占50%时最高,而与牛鞭草组合时则以50%最低。当鲁梅克斯-三叶草与除黑麦草之外的牧草组合时,产气速率随鲁梅克斯-三叶草所占比例的上升而上升,与黑麦草组合时,产气速率则呈相反方向变化,但不同组合比例间无显著差异。当鲁梅克斯-三叶草与除皇竹、桂牧1号、牛鞭草和冬牧70以外的禾本科牧草组合时,不同比例的牧草组合不同发酵时间点之间的正组合效应达到了显著水平。结论认为,3种牧草间组合可不同程度地提高牧草的体外发酵产气量。  相似文献   

18.
    
This study was conducted to investigate the effects of increasing levels of extracts of Byrsonima crassifolia, Celtis pallida, Enterolobium cyclocarpum, Fraxinus excelsior, Ficus trigonata, Phoradendrom brevifolium and Prunus domestica on in vitro gas production (GP) and ruminal fermentation of a high concentrate diet. Plant extracts were prepared at 1 g dry matter (DM)/8 mL of solvent mixture (methanol : ethanol : water, 1:1:8) and added at levels of 0, 0.6, 1.2 and 1.8 mL/g DM of a high concentrate diet. In vitro GP was recorded at 2, 4, 6, 8, 10, 12, 24, 48 and 72 h of incubation. Increasing addition of extracts linearly increased (P < 0.001), the GP24, GP48 and GP72 (mL/g DM), and linearly decreased (P < 0.001), the discrete GP lag time. Moreover, increasing extract doses linearly increased (P < 0.001) the asymptotic GP and decreased (P < 0.001) the rate of GP. GP6 was not impacted by treatments and GP12 increased linearly (P = 0.01) with increasing addition of extracts. Rumen pH declined linearly (P < 0.05) with increasing doses of extracts added. As no interactions (P > 0.05) occurred between the extracts and doses, it could be conclude that all extracts positively modified rumen fermentation at doses of 1.2 to 1.8 mL extract/g diet DM.  相似文献   

19.
    
Native Lantang and commercial Duroc pigs were used as animal models to evaluate the differences existing in dietary fiber utilization ability between breeds. Animals were fed the same diet from weaning (4 weeks) to 4 months of age. Neutral detergent fiber (NDF) from wheat bran (as substrate) and fecal samples from the two breeds (as inoculum) were used in an in vitro gas production trial. Results showed that cumulative and maximum gas productions were higher in inocula from Lantang than those from the Duroc breed (P < 0.05). The degradation capacity of NDF for microbiome from Lantang fecal samples were significantly higher compared to Duroc (P < 0.01). The total quantity of short‐chain fatty acids and its constituents from the fermentation liquors were different between breeds, suggesting that the dynamic characteristics of fermentation differed between the two breeds. The PCR denaturing gradient gel electrophoresis fingerprint and cluster analysis demonstrated that microbial communities of the two breeds were separated into two clusters and the bacterial community structure of large intestine among the two breed of pigs was different. Our results concluded that Lantang had higher dietary fiber degradation capacity than Duroc pigs, and the higher degradation capacity for the former breed was due to differences in the inherent microbial community in their respective large intestines.  相似文献   

20.
体外产气技术是反刍动物饲料营养价值评定中常用的方法之一。文章比较了体内法、瘤胃尼龙袋法和体外法在反刍动物营养价值评定中的特点,并着重从体外产气技术的技术细节、测定装置和应用范围进行了全面的论述,为该技术的标准化和自动化提供参考。  相似文献   

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