Effect of pasture allowance and supplementation with maize silage and concentrates differing in crude protein concentration on milk production and nitrogen excretion by dairy cows

Sixty cows (40 multiparous and 20 primiparous) averaging 140 days in milk (DIM) were assigned to five treatments to evaluate the effect of pasture allowance and supplements of a) maize silage b) high crude protein concentrate and, c) low crude protein concentrate on milk production and nitrogen (N) excretion. Two of the treatments (HG and LG) were offered herbage only (allowances of 20 and 15 kg dry matter (DM) per cow/day, respectively) while the remaining three treatments were offered a herbage allowance of 15 kg DM per cow/day plus 4 kg DM per cow/day of maize silage (M), a high CP concentrate (CP concentration of 194 g/kg DM) (HC), or citrus pulp (CP concentration of 69 g/kg DM) (LC). Total DM intake (DMI) was similar for HG, M, HC, and LC but was lower for LG (15.2 kg DM per cow/day) than HC (17.4 kg DM per cow/day). The reduction in pasture DMI per kg of supplement DM ingested was 0.44, 0.45, and 0.54 kg for cows offered maize silage, high CP concentrate, and low CP concentrate, respectively. Milk yield was greater for the supplemented treatments (23.7–24.7 kg/day) than for LG (20.7 kg/day) but not for HG (23.2 kg/day). Milk fat concentration was greater for HC (35.3 g/kg) than for HG, M, and LC but not greater than LG, while milk protein concentration was greater for HG (34.8 g/kg) than for LG and HC but not greater than M and LC. The greatest levels of N and PDIN intake were recorded for HG (662 and 2502 g/day) and HC (654 and 2506 g/day) which were greater than LC but not greater than LG and M. Treatment HC recorded the highest PDIE intake (1743 g/day) which was greater than LG, M and LC but not greater than HG. Output of N in milk was greater on HC (134 g/day) than on LG but was not greater than on HG, M, and LC. Faecal N excretion was greater on HG (171 g/day) than on all other treatments while estimated urinary N excretion was greater on HG and HC than LC (320 g/day). Treatment LC had a higher proportion of N output in milk (0.23) than treatment LG but not higher than HG, M and HC treatments. Urinary N expressed as a proportion of total N excretion was lower for HG (0.68) than all other treatments. The results of this study show that there is a large response in milk production to supplementing cows on a restricted grass allowance and that cows offered low CP supplements had similar levels of production to those offered high CP supplements. Nitrogen utilisation was improved by offering supplements of lower CP content.     

Effects of feeding early‐harvested orchardgrass–perennial ryegrass mixed silage instead of heading stage harvested timothy silage on digestion and milk production in dairy cows

We evaluated the effects of replacement of heading stage harvested timothy silage with early‐harvested orchardgrass–perennial ryegrass mixed (OP) silage while maintaining or reducing concentrate input on dry matter intake (DMI), milk production, nutrient digestibility, and N balance in dairy cows. Nine multiparous Holstein cows were used in a replicated 3 × 3 Latin square design with three dietary treatments: TYL, a diet containing timothy silage where forage‐to‐concentrate ratio (FC) was 50:50; OPL, a diet containing OP silage where FC ratio was 50:50; and OPH, a diet containing OP silage where FC ratio was 60:40. We observed that an equal replacement of timothy with OP silage increased DMI, milk yield, milk protein production, and nutrient digestibility but decreased milk fat content (TYL versus OPL). We observed that replacing timothy with OP silage while reducing concentrate input increased milk fat and protein yield, nutrient digestibility, and feed efficiency and reduced urinary N loss with no effect on DMI or milk fat content (TYL versus OPH). These results show that replacing timothy with OP silage can be a good approach to improve milk production, feed efficiency, and N utilization and reduce concentrate input. However, milk fat depression should be considered when an equal substitution is performed.     

The effect of energy supplementation on nitrogen utilization in lactating dairy cows fed grass silage diets

An experiment was conducted to examine the effect that various isoenergetic diets, containing different quantities of soluble carbohydrate and fiber and different types of starch, have on nitrogen (N) balances. Six lactating dairy cows in early to midlactation consuming grass silage diets with not less than 600 g/kg total DMI as forage were used in the experiment. Four concentrates were prepared that had higher amounts of either fiber, soluble sugars, corn (low degradable starch source), or barley (high degradable starch source). Overall N utilization by the cows was poor, rarely exceeding 0.30 g milk N/g of dietary N intake. Fecal N outputs accounted for more than half of total N excreted in all treatments except for diets supplemented with high degradable starch, in which urinary N excretion was significantly higher compared with the other treatments. Milk yield was unaffected by concentrate type, averaging 19.9 kg/d, but milk protein content decreased from 32.9 for starch-based diets to 30.9 and 30.0 g/kg for the soluble sugar- and fiber-based diets, respectively. The efficiency of N utilization improved in the low degradable starch treatment, which had lower N excretion (65%) and higher protein concentration in milk. Furthermore, feeding cows corn-based concentrates reduced urinary N excretion by almost 30% compared with barley-based concentrates; therefore, feeding corn-based diets is recommended for the reduction of nitrogen pollution in lactating dairy cows.     

Response of lactating dairy cows to degree of steam‐flaked barley grain in low‐forage diets

This study was conducted to investigate the effects of processing method (grinding vs. steam flaking) and increasing densities of steam‐flaked barley grain on dry matter intake (DMI), rumen pH and fermentation characteristics, digestibility of dry matter in the total digestive tract (DDTT), and milk production of dairy cows. Eight multiparous mid‐lactation Holstein cows averaging 103 ± 24 DIM, 44.5 ± 4.7 kg milk/day and weighing 611 ± 43 kg at the start of the experiment were used in a replicated 4 × 4 Latin square design with 21‐day periods. Cows were fed diets consisting of (DM basis) 23.8% corn silage, 13.5% chopped alfalfa hay and 62.7% concentrate. The dietary treatments were either ground barley (GB) using a hammer mill or steam‐flaked barley (SFB) – varying density at 390, 340 or 290 g/l. Processing method (GB vs. SFB) did not affect DMI (23.6 kg/day on average), DDTT (71.0% on average), milk yield (43.4 kg/day on average), milk components, rumen pH and molar proportions of acetate, propionate, butyrate and sorting activity. Ruminal isovalerate concentration tended (p = 0.06) to be higher for cows fed GB than those fed SFB‐based diets. Decreasing the density of SFB from 390, 340 to 290 g/l tended to linearly increase DMI (p = 0.09), decrease total solids percentage of milk (p = 0.10) and linearly decreased milk urea nitrogen (12.8, 12.4 and 12.1 mg/dl; p = 0.04); also, the sorting index (SI) of the particles retained on the 19.0‐mm sieve without affecting the SI of the particles retained on 8.0‐mm, 1.18‐mm or passed through 1.18‐mm sieve (p = 0.05). These results indicated the limited effects of processing method (grinding vs. steam flaking) and densities of SFB (390, 290 or 290 g/l) on cows’ performance and feed utilization for dairy cows fed low‐forage diets. Therefore, both processing methods could be recommended under current feeding conditions of dairy cows.     

Dairy cows fed on tropical legume forages: effects on milk yield,nutrients use efficiency and profitability

Two trials with multiparous dairy cows were conducted. Experiment 1 tested the effects of increasing forage proportion in the diet (500, 600, and 700 g/kg DM) when a mixed sorghum (Sorghum bicolor) and jackbean (Cannavalia ensiformis) silage was used as forage. Experiment 2 studied the substitution of sorghum silage and soybean meal by jackbean silage or fresh cowpea (Vigna unguiculata) forage in the diet. All diets were iso-energetic and iso-proteic. In each experiment, 30 cows were used and separated into three groups. In experiment 1, there were no differences in dry matter intake (DMI), milk yield (MY), or apparent total tract digestibility (aTTd) among the three diets, but milk fat content increased with increasing forage proportion, even though the similar neutral detergent fiber of all diets. Nitrogen use efficiency was highest in the diet containing 600 g forage/kg DM, and some evidence was observed for a better profitability with this forage proportion. In experiment 2, feeding legumes increased DMI despite no effects on aTTd. Milk yield increased in line with DMI, with a larger increase for the fresh cowpea. Nitrogen use efficiency and milk composition were not affected by the diets. The increased MY and lower feed costs increased the economic benefits when feeding legumes, particularly when feeding fresh cowpea. Feeding fresh cowpea or jackbean silage to dairy cows appears to be an alternative to soybean as protein source, ideally at a forage proportions of 600 g/kg DM, without altering milk yield and quality and increasing the farm profitability.     

Milk production performance of Friesian-Holstein cows fed diets containing Medicago sativa, Centrosema pubescens, or groundnut haulms (Arachis hypogaea)

To investigate the effect of feeding dairy cows diets containing lucerne hay, centrosema hay, and groundnut haulms (crop residue) on dry matter intake (DMI), milk yield, and milk composition, nine multiparous Friesian-Holstein cows in their mid-lactation stage were used in a 3?×?3 crossover design replicated three times. Dairy cows fed lucerne hay had significantly (p?<?0.001) higher DMI than dairy cows fed centrosema hay. DMI for cows fed groundnut haulms and lucerne hay was not significantly different. Daily milk yield for dairy cows fed diet containing lucerne hay was significantly (p?<?0.01) higher than that for dairy cows fed diets containing groundnut haulms or centrosema hay. Milk composition and body condition scores of the cows were not significantly affected by either lucerne hay, groundnut haulms, or centrosema hay. Overall, the results in this study indicated that feeding dairy cows diets containing lucerne hay increased milk yield.     

Effect of citrus pulp silage feeding on concentration of beta‐cryptoxanthin in plasma and milk of dairy cows

Citrus pulp is known to contain a functional molecule of beta‐cryptoxanthin which is one of the carotenoids showing anti‐oxidative capacity. Influences of citrus pulp silage feeding to dairy cows on beta‐cryptoxanthin concentration in plasma, other blood properties and milking performances were investigated. Four Holstein cows were fed total mixed ration (TMR) containing citrus pulp silage 20% dry matter (DM) for 2 weeks with free access to the TMR. Dry mater intake, milk production and milk components 2 weeks later were not altered compared with those of the control group without citrus pulp silage. Activities of aspartate aminotransferase, alanin aminotransferase and gamma‐glutamyltranspeptidase in plasma were not affected by feeding of citrus pulp silage. Concentrations of protein, albumin, sulfhydryl residue, ascorbic acid, thio‐barbituric acid reactive substance and urea nitrogen in plasma were also not altered by citrus pulp silage feeding. Concentration of beta‐cryptoxanthin in plasma was increased approximately 20‐fold compared with the control group (P < 0.05). Content of beta‐cryptxanthin in pooled milk fat fraction was also increased approximately three times compared with that of the control group. Feeding of TMR containing citrus pulp silage 15% DM for 30 days to eight dairy cows also increased plasma beta‐cryptoxanthin concentration 30‐fold compared with that before feeding.     

Effects of a Brown‐midrib corn hybrid on nutrient digestibility in wethers and on dry matter intake,performance, rumen and blood variables in dairy cows

The aim of the present trials was to determine the effect of an experimental Brown‐midrib (Bm) corn hybrid in relation to a commercial corn hybrid (Con) on digestibility in wethers and on dry matter intake (DMI), milk yield and milk composition in dairy cows. Digestibility of crude fibre (CF), neutral detergent fibre (NDFom) and acid detergent fibre (ADFom) were higher for Bm (CF Con: 57.8%; Bm: 67.2%; NDFom Con: 56.8%; Bm: 64.8%; ADFom Con: 52.0%; Bm: 63.9%), but concentration of net energy for lactation did not differ (Con: 6.4 MJ/kg DM; Bm: 6.3 MJ/kg DM). A total of 64 lactating German Holstein cows were assigned to one of the two dietary treatments Con or Bm according to milk yield, lactation number, days in milk and live weight. In Trial 1, cows were fed a total mixed ration consisting of 50% corn silage (Con or Bm) and 50% concentrate on dry matter (DM) basis. In Trial 2, the same animals were fed the respective silage for ad libitum intake and 5.3 kg of concentrate DM per animal per day. In Trial 1, DMI and milk‐fat content were decreased significantly for the Bm‐treatment (DMI Con: 22.5 kg/day; Bm: 21.5 kg/day; milk fat Con: 3.8%; Bm: 3.3%). In Trial 2, milk yield and fat‐corrected milk (FCM) were increased significantly, whereas milk‐fat% was decreased significantly (milk yield Con: 25.8 kg/day; Bm: 29.4 kg/day; FCM Con: 27.2 kg/day; Bm: 29.6 kg/day; fat Con: 4.4%; Bm: 4.0%). Diets did not influence ruminal pH or temperature. Diets, furthermore, did not influence rumination in either trial. Additional research on digestibility and rumen fermentation should, however, be carried out using dairy cows at respective intake levels as trials with wethers cannot be transferred to high‐yielding ad libitum fed cows.     

Effects of feeding alkaline hydrogen peroxide-treated wheat straw-based diets on intake, digestion, ruminal fermentation, and production responses by mid-lactation dairy cows.

The objective of this experiment was to determine the effects of feeding different levels of alkaline hydrogen peroxide-treated wheat straw (AHP-WS) in the diet on feed intake, nutrient digestion, ruminal fermentation, and production responses in mid-lactation dairy cows. Eight Holstein cows, averaging 147 d postpartum, were used in two replications of a 4 x 4 Latin square design. Complete mixed diets consisted of 70% forage and 30% concentrate (DM basis) with various levels of AHP-WS, alfalfa haylage, and corn silage as forage sources. Treatments contained 0 (control), 20.0, 40.1, or 60.0% AHP-WS in the diet. A quadratic effect (P = .08) of AHP-WS level on DMI was noted, with values of 2.16, 22.3, 20.8, and 18.9 kg/d for the control, 20.0, 40.1, and 60.0% AHP-WS treatments, respectively. Apparent digestibilities of DM, OM, CP, and ADF were not affected (P greater than .10) by replacing haylage and corn silage with increasing amounts of AHP-WS in the diet, but there was a linear increase (P = .03) in NDF digestibility (44% for control vs 59% for the 60.0% AHP-WS diet) and a parallel decrease (P less than .05) in cell content digestibility (82 vs 70% for these two diets). Yields of milk and 4% fat-corrected milk (FCM) were decreased (quadratic; P = .0001) as the level of AHP-WS increased in the diet. The addition of AHP-WS to the diet decreased the milk fat percentage from 3.72 to 3.60% (quadratic; P = .05) and decreased milk protein percentage from 3.27 to 3.13% (linear; P = .0001). Cows fed the higher levels of AHP-WS had linear increases (P = .0001) in ruminal concentrations of total VFA (128.0 mM for control vs 136.0 mM for the 60.0% AHP-WS treatment) and molar proportion of acetate, resulting in a quadratic effect (P less than .0001) on the acetate:propionate ratio. These data indicate that feeding the 40.1 and 60.0% AHP-WS diets lowered digestible DM and OM intakes, which resulted in reduced 4% FCM yield as nutrient intakes were decreased compared with cows fed the 20.0% AHP-WS diet or the control diet containing alfalfa haylage and corn silage. Although substituting AHP-WS for haylage and corn silage increased NDF digestibility and tended to increase digestible NDF intake, milk production was depressed because digestible DMI decreased.     

Analyses of factors affecting dry matter intake of lactating dairy cows

An experiment was conducted to analyze feed, climate and animal factors affecting dry matter intake (DMI) in lactating dairy cows. Sixteen lactating Holstein cows, with parity from 1 to 6, were assigned to a feeding trial for 2 years, comprising 31 lactations. The animals were fed Italian ryegrass silage, oat hay, alfalfa hay, beet pulp and three types of concentrate. The data, pooled and classified by stage of lactation, season of lactation and parity were analyzed by stepwise multiple regression to determine the nature and extent of factors affecting DMI. A total of 45 prediction equations for DMI were derived. Energy‐corrected milk yield or milk yield was selected as the primary factor of DMI in all the equations in which the ratio of contribution (R²) varied from 0.26 to 0.67. The dietary concentration of organic cell wall, crude fiber, crude protein, organic b fraction, forage to concentrate ratio, average ambient temperature and temperature–humidity index were selected as the secondary factors affecting DMI for pooled data, late lactation (251–350 days of lactation), summer (June–August), spring (March–May), ≥4th lactation, autumn (September–November) and 3rd lactation, respectively, and improved R² up to 0.77. Except for an impact of bodyweight in several equations, feed and climatic factors significantly improved prediction equations effectively for data classified in different ways. To estimate DMI accurately in lactating dairy cows, feed and climatic factors should be considered for specific conditions.     

Development of a model for the prediction of feed intake by dairy cows 2. Evaluation of prediction accuracy

In a previous paper we have proposed a new concept of a model for the prediction of feed intake by Holstein Friesian dairy cows (Zom et al., 2011). This model predicts feed intake from feed composition and digestibility and the cow's lactation number, stage of lactation and pregnancy. Contrary to many other often used models, this does not include animal performance (milk yield, bodyweight) to predict feed intake. However, BW and MY are highly correlated with DMI. Therefore, the objective of present study was to evaluate the accuracy and robustness of the novel feed intake model and to compare its accuracy and robustness with four other commonly used models for the prediction of feed intake.An evaluation was performed using an independent dataset containing 8974 weekly means of DMI from 348 individual cows observed in 6 feeding experiments including a wide range of diets and management practices was used in this study. Sub-datasets were formed by combining the DMI data by experiment, lactation number, lactation week, and maize silage to grass silage ratios in order to compare the accuracy of the intake models for different feeding practices and groups of cows using mean square prediction error (MSPE) and relative prediction error (RPE) as criteria.The novel model was most accurate as indicated by the MSPEs and RPEs for the whole dataset and the most of the sub-datasets. The results prove that the model of Zom et al. (2011) is able to predict DMI without the use of milk yield or body weight as inputs. It was concluded that novel model was robust and can be applied to various diets and feeding management situations in lactating HF cows.     

Effects of length of cut and mechanical processing on utilization of corn silage harvested at the black line stage of maturity by lactating dairy cows

The effects of length of cut and mechanical processing on corn silage utilization by dairy cows were evaluated. Corn silage treatments were harvested at the black line stage of maturity and chopped at a theoretical length (TLC) of 9.5 mm without processing (Control) or at a TLC of 19 mm with processing at roller clearances of 1, 3, or 5 mm. Eight multiparous Holstein cows were assigned in a replicated 4 × 4 Latin square design with 21‐day periods. Corn silage treatments were fed in diets containing 78.3% corn silage and 21.7% soybean meal (DM basis). Treatments had no significant effects on DMI, milk and 4% FCM production. The efficiency of converting DMI to FCM tended to be greater with processing at a roller clearance of 1 and 3 mm than at other clearances. Apparent total tract digestibility of starch tended to be lowest for cows fed control silage, and increased as roller clearance decreased. Ruminal ammonia concentrations in cows fed control silage were numerically higher than in cows fed proccesed silages. These results suggest that when corn silage is harvested at the black line of maturity, roller clearance should be 3 mm or less with a TLC of 19 mm.     

Effect of anthocyanin‐rich corn silage on digestibility,milk production and plasma enzyme activities in lactating dairy cows

Anthocyanin in purple corn (Zea mays L.) has been reported to show several functional and biological attributes, displaying antioxidant, antiobesity and antidiabetic effects in monogastric animals. The objective of this study was to investigate the effect of feeding anthocyanin‐rich corn (Zea mays L., Choko C922) silage on digestibility, milk production and plasma enzyme activities in lactating dairy cows. The cows were fed diets based on the control corn or the anthocyanin‐rich corn silage (AR treatment) in a crossover design. The anthocyanin‐rich corn silage‐based diet had a lower starch content, nutrient digestibility and total digestible nutrients content when compared to the control diet. The milk yield, lactose and solids‐not‐fat contents in the AR‐treatment cows were lower than in the control cows. The feeding of the anthocyanin‐rich corn silage led to a reduction in aspartate aminotransferase (AST) activity and an increase in superoxide dismutase (SOD) activity in the plasma. These data suggest that the anthocyanin‐rich corn has a lowering effect on AST activity with concomitant enhancement of SOD activity in lactating dairy cows. However, a new variety of anthocyanin‐rich corn with good nutritional value is needed for practical use as a ruminant feed.     

Effect of replacing corn with brown rice grain in a total mixed ration silage on milk production,ruminal fermentation and nitrogen balance in lactating dairy cows

Nine multiparous Holstein cows were used in a replicated 3 × 3 Latin square design to determine the effects of substituting corn grain with brown rice (BR) grain in total mixed ration (TMR) silage on milk yield, ruminal fermentation and nitrogen (N) balance. The TMR silages were made from the ensiling of TMR containing (dry matter basis) 50.1% forage in rice silage and corn silage combination, and 49.9% concentrate. The grain portion of the diets contained 31.2% steam‐flaked corn, 31.2% steam‐flaked BR or an equal mixture of corn and BR. Dietary treatments did not affect dry matter intake, milk yield and milk fat, protein and lactose yields. The ruminal pH and total volatile fatty acid concentrations were not affected by dietary treatment. The urinary N excretion decreased linearly (P < 0.01) in response to increased levels of BR, with no dietary effect on N intake, N secretion in milk and fecal N excretion. Our results indicate that steam‐flaked BR is a suitable replacement for steam‐flaked corn in dairy cow diets, and that it can be included in rations to a level of at least 31.2% of dry matter without adverse effects on milk production, when cows were fed rice silage and corn silage‐based diets.     

ORIGINAL ARTICLE: Effects of long‐term feeding of genetically modified corn (event MON810) on the performance of lactating dairy cows

A long‐term study over 25 months was conducted to evaluate the effects of genetically modified corn on performance of lactating dairy cows. Thirty‐six dairy cows were assigned to two feeding groups and fed with diets based on whole‐crop silage, kernels and whole‐crop cobs from Bt‐corn (Bt‐MON810) or its isogenic not genetically modified counterpart (CON) as main components. The study included two consecutive lactations. There were no differences in the chemical composition and estimated net energy content of Bt‐MON810 and CON corn components and diets. CON feed samples were negative for the presence of Cry1Ab protein, while in Bt‐MON810 feed samples the Cry1Ab protein was detected. Cows fed Bt‐MON810 corn had a daily Cry1Ab protein intake of 6.0 mg in the first lactation and 6.1 mg in the second lactation of the trial. Dry matter intake (DMI) was 18.8 and 20.7 kg/cow per day in the first and the second lactation of the trial, with no treatment differences. Similarly, milk yield (23.8 and 29.0 kg/cow per day in the first and the second lactation of the trial) was not affected by dietary treatment. There were no consistent effects of feeding MON810 or its isogenic CON on milk composition or body condition. Thus, the present long‐term study demonstrated the compositional and nutritional equivalence of Bt‐MON810 and its isogenic CON.     

Effects of forage-to-concentrate ratio in prepartum diet on the dry matter intake and milk yield of periparturient cows during hot weather

The objective of this experiment was to study the effect of prepartum diets on dry matter intake (DMI) around parturition and on milk production in early lactation during a hot summer. Nine cows due to calve in July or August were assigned to two groups and fed either control (group C; four cows) or high‐concentrate (group H; five cows) diets to meet 110% of their energy requirements from 18 days before the expected calving date to parturition. The proportion of concentrate in the control diet was 35%, and that in the high‐concentrate diet was 45%. For 21 days after parturition, all cows were fed the same diets for lactation ad libitum. The DMI of cows in group H almost met their energy requirements. In group C, the DMI was decreased relative to group H and the concentration of plasma non‐esterified fatty acids was significantly higher (P < 0.05) before parturition. Therefore, the cows in group C were thought to be energy deficient before parturition. After parturition, there were no significant differences in DMI between the two groups. The milk yield of cows in group H tended to be higher than that of cows in group C at 21 days after parturition. Milk protein yield was significantly higher (P < 0.05) in group H 21 days after parturition relative to group C. There were no significant differences in milk composition between the two groups.     

Ammonia and urea in corn silage-based complete mixed diets for dairy cows

Source of supplemental N was evaluated in three corn silage-based complete mixed diets (CMD) fed to lactating dairy cows. Diets were formulated to be isonitrogenous and contained 60% corn silage and 40% concentrate on a dry matter basis. Diets were: CMD-A, ammoniated corn silage (ammonia provided 16% of total N) plus concentrate; CMD-U, untreated corn silage and concentrate (16% of total N from urea) and CMD-S, untreated corn silage plus concentrate with soybean meal. Ammoniation at 1.0% of dry weight increased crude protein content of silage from 8.5 to 12.7%. Daily means for cows fed CMD-A, CMD-U and CMD-S were: 24.2, 24.3 and 24.5 kg of fat-corrected milk; 3.17, 3.10 and 3.15% milk protein; 3.41, 3.67 and 3.63% milk fat and 3.02, 2.99 and 3.02 kg intake of total dry matter/100 kg body weight. Differences were not significant except for reduced milk fat percentage for cows fed the ammoniated corn silage diet. Both anhydrous ammonia and urea at 16% of total dietary N were found to be practical and economical substitutes for part of the supplemental soy protein. When compared to CMD with all soybean protein, greater savings in feed costs resulted from inclusion of ammoniated corn silage than with inclusion of urea.     

Effects of Moringa oleifera silage on milk yield,nutrient digestibility and serum biochemical indexes of lactating dairy cows

This study investigated the effects of Moringa oleifera (MO) as a partial substitute of alfalfa hay on milk yield, nutrient apparent digestibility and serum biochemical indexes of dairy cows. MO was harvested at 120 days post‐seeding. Fresh MO was cut, mixed with chopped oat hay (425:575 on a DM basis), ensiled and stored for 60 days. Sixty healthy Holstein dairy cows were allocated to one of three groups: NM (no MO or control), LM (low MO; 25% alfalfa hay and 50% maize silage were replaced by MO silage) or HM (high MO; 50% alfalfa hay and 100% maize silage were replaced by MO silage). The feeding trial lasted 35 days. The LM and HM diets did not affect dry matter (DM) intake, milk yield or milk composition (lactose, milk fat, milk protein and somatic cell count). The apparent digestibility of DM and NDF was lower for HM group than NM group. Additionally, there were no significant differences in serum biochemical indexes between the LM and NM groups. The HM group had lower serum concentrations of total cholesterol, high‐density lipoprotein cholesterol, and low‐density lipoprotein cholesterol and higher serum concentrations of urea than the NM group. The partial replacement of alfalfa hay (≤50%) and maize silage with MO silage had no negative effects on milk yield, in vivo nutrient apparent digestibility or serum biochemical indexes of lactating cows.     

EM发酵玉米秸秆和青贮玉米秸秆对奶牛生产性能的对比试验

本试验应用EM发酵玉米秸秆和青贮玉米秸秆饲喂奶牛,以观察两种方法处理的玉米秸秆对奶牛生产性能及经济效益的影响效果。结果表明,用EM发酵的玉米秸秆饲喂奶牛较青贮玉米秸秆饲喂奶牛,每天每头奶牛采食量提高1.03%,平均产奶量提高2.02%,经济效益提高0.13元;EM原液发酵的玉米秸秆和青贮玉米秸秆1∶1混合饲喂奶牛,每天每头奶牛采食量、平均产奶量、经济效益分别比饲喂青贮玉米秸秆的奶牛提高2.85%、2.99%和1.15元。     

Comparison of the performance of Holstein-Friesian and Jersey×Holstein-Friesian crossbred dairy cows within three contrasting grassland-based systems of milk production

The performance of spring calving Holstein-Friesian (HF) and Jersey×Holstein-Friesian (J×HF) dairy cows was examined during three successive years (mean of 35 HF cows and 31 J×HF cows per year). Throughout the experiment cows were managed on one of three grassland-based systems of milk production, namely low concentrate (LC), medium concentrate (MC) or high concentrate (HC). Post-calving, cows were housed and offered grass silage, supplemented with 6.0, 8.0 and 10.0 kg concentrate/cow/d in systems LC, MC and HC, respectively (mean period from calving to start of full time grazing, 69 days). During the grazing period target concentrate feed levels were 0, 2.5 and 5.0 kg/cow/d for systems LC, MC and HC, respectively (mean period from start of full time grazing to start full time re-housing, 206 days). Full lactation concentrate inputs were 530, 1092 and 1667 kg/cow, in systems LC, MC and HC, respectively. There were no significant genotype×system interactions for any of the milk production parameters examined. Food intake during the confinement and grazing periods was unaffected by genotype. Milk yield was highest with HF cows while milk fat and milk protein content were highest with the J×HF cows (P<0.001). Genotype had no effect on fat plus protein yield. Milk yield and fat plus protein yield were higher with systems MC and HC than with LC (P<0.001). HF cows were on average 44 kg heavier than J×HF cows, while the mean condition score of the J×HF cows was approximately 0.2 units higher than that of the HF cows (P<0.001). Live weight and condition score changes during the lactation followed similar trends with both genotypes. The J×HF cows had fewer days to first observed heat (P<0.05), a higher conception rate to first service (P<0.01), first plus second service (P<0.001), and a higher pregnancy rate at the end of the breeding season (P<0.05). Although mean somatic cell score was unaffected by genotype, the proportion of cows with one or more cases of mastitis was lower with the J×HF cows (P<0.05). In summary, while the J×HF cows had improved fertility performance compared to the HF cows, both genotypes exhibited similar levels of tissue mobilisation and deposition throughout the lactation, while there was no evidence of a genotype×grassland system interaction for any of the milk production parameters examined.