The sensitivity of water extractable soil organic carbon fractions to land use in three soil types

Soil organic carbon (SOC) has a high impact on the sustainability of ecosystems, global environmental processes, soil quality and agriculture. Long-term tillage usually leads to SOC depletion. The purpose of this study was to determine the impact of different land uses on water extractable organic carbon (WEOC) fractions and to evaluate the interaction between the WEOC fractions and other soil properties. Using an extraction procedure at 20°C and 80°C, two fractions were obtained: a cold water extractable organic carbon (CWEOC) and a hot water extractable organic carbon (HWEOC). The results suggest that there is a significant impact from different land uses on WEOC. A lower relative contribution of WEOC in SOC and a lower concentration of labile WEOC fractions are contained in arable soil compared to forestlands. Chernozem soil was characterized by a lower relative contribution of WEOC to the SOC and thus higher SOC stability in contrast to Solonetz and Vertisol soils. Both CWEOC and HWEOC are highly associated with SOC in the silt and clay fraction (<53 µm) and were slightly associated with SOC in the macroaggregate classes. The WEOC fractions were highly and positively correlated with the SOC and mean weight diameter.     

黄土高原坝地深层土壤有机碳稳定性研究

淤地坝作为黄土高原重要的碳储库,其深层土壤有机碳稳定性在很大程度上影响坝地土壤储碳能力和碳排放。以黄土丘陵区不同利用年限的坝地为对象,从坝地剖面土壤有机碳含量及其组分入手,研究不同利用年限、不同沉积深度下,土壤有机碳含量及其稳定性的变化特征和影响因素。结果表明：（1）坝地深层土壤有机碳（SOC）含量低于该区坡耕地表层土壤有机碳含量,并未呈现明显的有机碳富集现象。随利用年限增加,坝地SOC含量呈增加趋势。（2）不同利用年限坝地的SOC、易氧化碳（EOC）、微生物量碳（MBC）、水溶性碳（DOC）含量呈现出明显的表聚现象。MBC、DOC和EOC含量在土壤0—60 cm内较高。（3）相较于坝地浅层土壤而言,坝地深层土壤有机碳具有较高的稳定性,长期耕作会降低坝地深层土壤有机碳稳定性。（4）坝地浅层和深层土壤有机碳稳定性变化的主导因素不同。浅层土壤有机碳稳定性主要受土层深度、有机碳含量和黏粒含量的影响,分别能解释其变异的50.4%,19.6%和11.8%;深层土壤有机碳稳定性主要受有机碳含量、土壤含水量和利用年限的影响,分别能解释其变异的38.9%,33.9%和11.8%。     

Loss of labile organic carbon from subsoil due to land-use changes in subtropical China

Topsoil carbon (C) stocks are known to decrease as a consequence of the conversion of natural ecosystems to plantations or croplands; however, the effect of land use change on subsoil C remains unknown. Here, we hypothesized that the effect of land use change on labile subsoil organic C may be even stronger than for topsoil due to upward concentration of plantations and crops root systems. We evaluated soil labile organic C fractions, including particulate organic carbon (POC) and its components [coarse POC and fine POC], light fraction organic carbon (LFOC), readily oxidizable organic carbon, dissolved organic carbon (DOC) and microbial biomass down to 100 cm soil depth from four typical land use systems in subtropical China. Decrease in fine root biomass was more pronounced below 20 cm than in the overlying topsoil (70% vs. 56% for plantation and 62% vs. 37% for orchard. respectively) driving a reduction in subsoil labile organic C stocks. Land use changes from natural forest to Chinese fir plantation, Chinese chestnut orchard, or sloping tillage reduced soil organic C stocks and that of its labile fractions both in top and subsoil (20–100 cm). POC reduction was mainly driven by a decrease in fine POC in topsoil, while DOC was mainly reduced in subsoil. Fine POC, LFOC and microbial biomass can be useful early indicators of changes in topsoil organic C. In contrast, LFOC and DOC are useful indicators for subsoil. Reduced proportions of fine POC, LFOC, DOC and microbial biomass to soil organic C reflected the decline in soil organic C quality caused by land use changes. We conclude that land use changes decrease C sequestration both in topsoil and subsoil, which is initially indicated by the labile soil organic C fractions.     

南方侵蚀治理区土壤碳分布及主控因素研究

研究侵蚀治理区土壤碳区域分布影响因素对揭示退化土壤有机碳恢复有重要的意义。本文以江西省兴国县侵蚀治理恢复区林地土壤有机碳为研究对象,评价了土壤类型和成土母质对土壤有机碳的影响及其主控作用。结果表明：兴国县侵蚀治理恢复区林地土壤有机碳受土壤类型和成土母质影响显著,其中黄红壤各土层土壤有机碳含量均显著高于红壤;千枚岩发育的土壤有机碳显著高于红砂岩和花岗岩。对比土壤类型和成土母质对土壤有机碳的影响作用大小发现,在表层0 ~ 20 cm土壤类型对土壤有机碳变异解释能力大于30%,成土母质的解释能力约为17%,土壤类型是影响表层土壤有机碳的主控因素;表下层20 ~ 30 cm 土壤有机碳变异的解释能力则表现为成土母质(28.8%)与土壤类型(27.5%)基本相当,同为主控因素。因此,兴国县侵蚀治理恢复区土壤类型和成土母质对土壤有机碳的影响作用不容忽视,合理地分区展开水土保持工作有利于退化土壤的碳恢复。     

Contribution of sorption,DOC transport and microbial interactions to the 14C age of a soil organic carbon profile: Insights from a calibrated process model

Profiles of soil organic carbon (SOC) are often characterized by a steep increase of ¹⁴C age with depth, often leading to subsoil ¹⁴C ages of more than 1000 years. These observations have generally been reproduced in SOC models by introducing a SOC pool that decomposes on the time-scale of millennia. The overemphasis of chemical recalcitrance as the major factor for the persistence of SOC was able to provide a mechanistic justification for these very low decomposition rates. The emerging view on SOC persistence, however, stresses that apart from molecular structure a multitude of mechanisms can lead to the long-term persistence of organic carbon in soils. These mechanisms, however, have not been incorporated into most models. Consequently, we developed the SOC profile model COMISSION which simulates vertically resolved SOC concentrations based on representations of microbial interactions, sorption to minerals, and vertical transport. We calibrated COMISSION using published concentrations of SOC, microbial biomass and mineral-associated OC (MOC), and in addition, ¹⁴C contents of SOC and MOC of a Haplic Podzol profile in North-Eastern Bavaria, Germany. In order to elucidate the contribution of the implemented processes to the ¹⁴C age in different parts of the profile, we performed model-experiments in which we switched off the limitation of SOC decomposition by microbes, sorptive stabilization on soil minerals, and dissolved OC (DOC) transport. By splitting all model pools into directly litter-derived carbon and microbe-derived organic carbon, we investigated the contribution of repeated microbial recycling to ¹⁴C ages throughout the profile. The model-experiments for this site lead to the following implications: Without rejuvenation by DOC transport, SOC in the subsoil would be on average 1700 ¹⁴C years older. Across the profile, SOC from microbial recycling is on average 1400 ¹⁴C years older than litter-derived SOC. Without microbial limitation of depolymerization, SOC in the subsoil would be on average 610 ¹⁴C years younger. Sorptive stabilization is responsible for relatively high ¹⁴C ages in the topsoil. The model-experiments further indicate that the high SOC concentrations in the Bh horizon are caused by the interplay between sorptive stabilization and microbial dynamics. Overall, the model-experiments demonstrate that the high ¹⁴C ages are not solely caused by slow turnover of a single pool, but that the increase of ¹⁴C ages along a soil profile up to ages >1000 years is the result of different mechanisms contributing to the overall persistence of SOC. The dominant reasons for the persistence of SOC are stabilization processes, followed by repeated microbial processing of SOC.     

A comparison of extraction procedures for water‐extractable organic matter in soils

The characteristics of dissolved organic matter (DOM) in soils are often determined through laboratory experiments. Many different protocols can be used to extract organic matter from soil. In this study, we used five air‐dried soils to compare three extraction methods for water‐extractable organic matter (WEOM) as follows: (i) pressurised hot‐water‐extractable organic carbon (PH‐WEOC), a percolation at high pressure and temperature; (ii) water‐extractable organic carbon (WEOC), a 1‐hour end‐over shaking; and (iii) leaching‐extractable organic carbon (LEOC), a leaching of soil columns at ambient conditions. We quantified the extraction yield of organic carbon; the quality of WEOM was characterized by UV absorbance, potential biodegradability (48‐day incubation) and parallel factor analysis (PARAFAC) modelling of fluorescence excitation emission matrices (FEEMs). Biodegradation of dissolved organic carbon (DOC) was described by two pools of organic C. The proportions of labile and stable DOC pools differed only slightly between the WEOC and LEOC methods, while PH‐WEOC contains more stable DOC. The mineralization rate constants of both labile and stable DOC pools were similar for the three methods. The FEEMs were decomposed into three components: two humic‐like fluorophores and a tryptophan‐like fluorophore. The effect of extraction method was poorly discriminant and the most similar procedures were PH‐WEOC and LEOC while WEOC extracts were depleted in humic‐like fluorophores. This study demonstrates that WEOM quality is primarily determined by soil characteristics and that the extraction method has a smaller, but still significant, impact on WEOM quality. Furthermore, we observed considerable interaction between extraction procedure and soil type, showing that method‐induced differences in WEOM quality vary with soil characteristics.     

Carbon isotopes of water‐extractable organic carbon in a depth profile of forest soil imply a dynamic relationship with soil carbon

Although considerable research has been conducted on the importance of recent litter compared with older soil organic matter as sources of dissolved organic carbon (DOC) in forest soils, a more thorough evaluation of this mechanism is necessary. We studied water‐extractable organic carbon (WEOC) in a soil profile under a cool‐temperate beech forest by analysing the isotopic composition (¹³C and ¹⁴C) of WEOC and its fractions after separation on a DAX‐8 resin. With depth, WEOC became more enriched in ¹³C, which reflects the increasing proportion of the hydrophilic, isotopically heavier fraction. The ¹⁴C content in WEOC and its fractions decreased with depth, paralleling the ¹⁴C trend in soil organic matter (SOM). These results indicate a dynamic equilibrium of WEOC and soil organic carbon. The dominant process maintaining the WEOC pool in the mineral soil appears to be the microbial release of water‐soluble compounds from the SOM, which alters in time‐scales of decades to centuries.     

水溶性有机碳在各种粘土底土中的吸附：土壤性质的影响

Clay-rich subsoils are added to sandy soils to improve crop yield and increase organic carbon (C) sequestration; however, little is known about the influence of clay subsoil properties on organic C sorption and desorption. Batch sorption experiments were conducted with nine clay subsoils with a range of properties. The clay subsoils were shaken for 16 h at 4 ^oC with water-extractable organic C (WEOC, 1 224 g C L^-1) from mature wheat residue at a soil to extract ratio of 1:10. After removal of the supernatant, the residual pellet was shaken with deionised water to determine organic C desorption. The WEOC sorption was positively correlated with smectite and illite contents, cation exchange capacity (CEC) and total organic C, but negatively correlated with kaolinite content. Desorption of WEOC expressed as a percentage of WEOC sorbed was negatively correlated with smectite and illite contents, CEC, total and exchangeable calcium (Ca) concentrations and clay content, but positively correlated with kaolinite content. The relative importance of these properties varied among soil types. The soils with a high WEOC sorption capacity had medium CEC and their dominant clay minerals were smectite and illite. In contrast, kaolinite was the dominant clay mineral in the soils with a low WEOC sorption capacity and low-to-medium CEC. However, most soils had properties which could increase WEOC sorption as well as those that could decrease WEOC sorption. The relative importance of properties increasing or decreasing WEOC sorption varied with soils. The soils with high desorption had a low total Ca concentration, low-to-medium CEC and low clay content, whereas the soils with low desorption were characterised by medium-to-high CEC and smectite and illite were the dominant clay minerals. We conclude that WEOC sorption and desorption depend not on a single property but rather a combination of several properties of the subsoils in this study.     

水分梯度对若尔盖高寒湿地土壤活性有机碳分布的影响

沿自然原因和人为原因形成的水分梯度,对若尔盖高寒湿地沼泽土和泥炭土的有机碳(SOC)和活性有机碳(LC)进行了研究。研究表明,若尔盖高寒湿地沼泽土有机碳和全氮沿水分梯度(减小)变化趋势一致,即在表层0—10cm湿润环境中的有机碳和全氮含量远高于淹水环境和过渡地带,而10—30 cm沿水分梯度差异变小。泥炭土的有机碳和氮素含量在湿润环境远大于淹水(流水)环境。这说明当时的挖沟排水疏干沼泽使得相当一部分土壤有机碳或者释放到大气中,或者随水流流失。沼泽土活性有机碳在表层0—10 cm沿水分梯度升高;在10—30 cm差异变小,与有机碳和氮素的变化趋势一致。泥炭土的活性有机碳沿水分梯度升高,与泥炭土有机碳和氮素变化趋势一致。这一方面反映了两种土壤类型成土过程的不同,另一方面也反映了自然原因和人为原因造成的差异。沼泽土的碳氮比沿水分梯度有降低的趋势而泥炭土的碳氮比沿水分梯度有升高的趋势。此外,高寒沼泽土碳氮比,pH值以及机械组成都是影响土壤有机碳,氮素和活性有机碳的重要因子。     

Specific Responses of Soil Microbial Residue Carbon to Long-Term Mineral Fertilizer Applications to Reddish Paddy Soils

Mineral nutrient inputs to soil may alter microbial activity and consequently influence the accumulation of microbial residues. In this study, we investigated the effects of application rates and ratios of mineral fertilizers on the microbial residue carbon(MRC) of reddish paddy soils after long-term(15-year) fertilizer applications in southern China. Contents of three soil amino sugars as microbial residue contents were determined and MRC were calculated based on amino sugars. Results showed that three individual amino sugar contents increased as fertilizer application rates increased until maximum values were reached at a rate of 450-59-187 kg ha~(-1) year~(-1)(N-P-K). The three amino sugar contents then declined significantly under the highest mineral fertilizer application rate of 675-88-280 kg ha~(-1) year~(-1)(N-P-K). In addition, to enhance the microbial residue contents, it was more beneficial to double P(N:P:K= 1:0.26:0.41) in fertilizers applied to the P-deficient reddish paddy soils than to double either N(N:P:K = 2:0.13:0.41) or K(N:P:K= 1:0.13:0.82). The contents of the three individual amino sugars and microbial residues under different fertilizer application rates and ratios were significantly and positively correlated with soil organic carbon(SOC), total N, total P, and p H. Increases in values of the fungal C to bacterial C ratios showed that soil organic matter(SOM) stability increased because of the fertilizer applications over the past 15 years. The contents and ratios of amino sugars can be used as indicators to evaluate the impact of mineral fertilizer applications on SOM dynamics in subtropical paddy soils. The results indicated that fertilizer applications at a rate of 450-59-187 kg ha~(-1) year~(-1)(N-P-K) may improve crop yields, SOC contents, and SOC stability in subtropical paddy soils.     

Soil carbon dynamics in saline and sodic soils: a review

Soil salinity (high levels of water-soluble salt) and sodicity (high levels of exchangeable sodium), called collectively salt-affected soils, affect approximately 932 million ha of land globally. Saline and sodic landscapes are subjected to modified hydrologic processes which can impact upon soil chemistry, carbon and nutrient cycling, and organic matter decomposition. The soil organic carbon (SOC) pool is the largest terrestrial carbon pool, with the level of SOC an important measure of a soil's health. Because the SOC pool is dependent on inputs from vegetation, the effects of salinity and sodicity on plant health adversely impacts upon SOC stocks in salt-affected areas, generally leading to less SOC. Saline and sodic soils are subjected to a number of opposing processes which affect the soil microbial biomass and microbial activity, changing CO₂ fluxes and the nature and delivery of nutrients to vegetation. Sodic soils compound SOC loss by increasing dispersion of aggregates, which increases SOC mineralisation, and increasing bulk density which restricts access to substrate for mineralisation. Saline conditions can increase the decomposability of soil organic matter but also restrict access to substrates due to flocculation of aggregates as a result of high concentrations of soluble salts. Saline and sodic soils usually contain carbonates, which complicates the carbon (C) dynamics. This paper reviews soil processes that commonly occur in saline and sodic soils, and their effect on C stocks and fluxes to identify the key issues involved in the decomposition of soil organic matter and soil aggregation processes which need to be addressed to fully understand C dynamics in salt-affected soils.     

森林转换对土壤活性有机碳组分的影响

为了研究林型转换对土壤活性有机碳组分的影响,在安徽皖南地区蔡家桥林场选取了马尾松次生林、湿地松人工林以及杉木人工林3种森林类型,分别采集了0—10,10—30,30—50 cm的土壤,测定了土壤有机碳(SOC)、颗粒有机碳(POC)、易氧化有机碳(EOC)、微生物生物量碳(MBC)、可溶性有机碳(DOC)以及土壤理化指标,分析了林型转换后土壤活性有机碳组分变化特征及其与土壤理化因子间的相关关系。结果表明：(1)马尾松次生林转换成湿地松人工林和杉木人工林后主要对0—10 cm土壤活性有机碳组分产生影响,其中土壤SOC,POC,EOC含量均在林型转换后出现下降,DOC含量上升,而MBC在林型转换前后无显著差异。(2)林型转换后各土层POC/SOC均出现下降,DOC和EOC占SOC比例总体呈升高趋势,MBC/SOC则未表现出明显规律。(3)土壤有机碳与活性碳组分以及TN,EC,NH⁺₄-N,NO^-₃-N均呈极显著正相关,各活性碳组分之间也存在极显著正相关关系(p<0.01)。综上,马尾松次生林转换成...     

Integrating soil analyses with frameworks for ecosystem services and organizational hierarchy of soil systems

Ecosystems provide various goods and services (provisioning, regulating, cultural, and supporting) that benefit humans both directly and indirectly. Soil plays an important role in ecosystem services therefore soil analyses can provide quantitative and qualitative data to evaluate ecosystem goods and services. Soil analyses must be integrated with the frameworks for ecosystem services and existing organizational hierarchy of soil systems to provide missing links to scale, time, degree of computation and complexity. This case study demonstrates the importance of evaluating soil organic carbon (SOC) within this newly proposed context using glaciated soils at the Cornell University Willsboro Research Farm in upstate New York as the example. The vertical distribu tion of SOC was analyzed quantitatively by soil depth class (topsoil versus subsoil), soil order, and other relevant variables that relate to the organizational hierarchy of soil systems.     

侵蚀区植被恢复过程中土壤有机碳稳定性的研究进展

探究土壤有机碳(SOC)的组成、来源和稳定性机制是深入认识陆地碳汇功能和应对气候变化的关键。“增加土壤碳汇”与“稳定现存土壤碳汇”都是提升陆地生态系统碳固持能力的重要方面,地位同等重要。与“增汇”研究成果丰硕相比,“稳汇”研究相对薄弱。侵蚀区进行植被恢复可以显著促进SOC积累,但由于侵蚀区存在碳素坡面侵蚀损失,其碳素积累效率低于其他生态系统类型区这一重要环节,导致目前有关侵蚀区及其水土保持植被恢复过程中SOC动态变化、稳定性及固持长期有效性等问题尚不清楚,微生物介导的SOC稳定机制尚未充分揭示。通过简要概括侵蚀区植被恢复过程中土壤碳素的积累效益和影响因素,综述植被恢复对土壤SOC及其活性组分稳定性的影响;在简要介绍土壤微生物在调控土壤碳素稳定性重要作用的基础上,梳理了基于微生物“碳泵”理论的土壤有机碳稳定性研究进展,特别是指出了随着植被恢复进程,侵蚀区土壤微生物介导的SOC动态变化,总结现有研究的不足。指出今后需要从研究对象(重点是西南石漠化区和南方红壤丘陵区)、研究内容(土壤微生物介导的SOC稳定状态和机制)、研究手段(借用微生物碳泵的理念,野外典型样地调查与室内培养手段相结合)和研...     

长期施肥对红壤旱地土壤活性有机碳和酶活性的影响

以江西进贤长期肥料定位试验为平台,研究了红壤旱地不同施肥措施对土壤微生物生物量、活性有机C、C库管理指数以及土壤酶活性的影响。研究结果表明:与不施肥和单施化肥土壤相比,施有机肥处理土壤的pH、CEC、有机C、全N、全P、无机N、速效P、速效K及土壤微生物生物量均显著增加,土壤活性有机C和C库管理指数也较试前土壤和其他处理土壤明显提高,此外,土壤的转化酶、脱氢酶、脲酶和酸性磷酸酶活性也较其他处理显著增加。土壤微生物生物量、活性有机C以及4种土壤酶活性之间的相关关系显著,且它们均与土壤有机C、全N、全P、无机N、速效P等土壤养分呈显著正相关。因此,红壤旱地通过长期施用有机肥或与无机肥配施,不仅能显著提高土壤有机质的数量和质量,而且能增加土壤微生物生物量和酶活性,从而显著提高土壤肥力和土壤持续生产力。     

不同地形条件下青藏高原农田土壤有机碳的分布特征

西北高寒地区农田土壤有机碳（SOC）储量的变化研究,可为东部农田SOC对气候和管理措施的响应提供预警信息。针对西部高原县域尺度上典型的地貌类型和土壤类型,对其耕层和剖面SOC进行了分析。结果表明, 青海省乐都县农田耕层（020 cm）SOC的变化范围为4.38 g/kg~20.81 g/kg,均值为11.29 g/kg,且不同土壤类型上表现出黑钙土（16.15 g/kg）＞栗钙土（10.53 g/kg）＞灰钙土（9.50 g/kg）的趋势。地形对耕层（020 cm）SOC含量没有显著影响,但深层（20100 cm）SOC因地形存在显著差异,在峁坡上,黑钙土、 栗钙土和灰钙土的深层（20100 cm）SOC分别比同种土壤类型的谷底深层土壤提高了111.5%、 62.5%和66.3%。农田SOC的垂直分布也因地形存在差异,同一种土壤类型在谷底其耕层（020 cm）SOC含量均比深层（20100 cm）高,峁坡上其深层（20100 cm）比耕层（020 cm）高,黑钙土、 栗钙土和灰钙土在谷底其耕层（020 cm）SOC含量分别比同一土壤类型的深层（20100 cm）土壤提高18.7%、 24.3%和153.5%,黑钙土、 栗钙土和灰钙土在峁坡上其深层（20100 cm）SOC含量分别比同一土壤类型耕层（020 cm）提高46.9%、 8.0%和1.0%。这一结果可为准确估算青藏高原农田SOC的变化提供参考。     

Filtration increases the correlation between water extractable organic carbon and soil microbial activity

A study was carried out in order to establish the relationship between the water extractable organic carbon (WEOC) content of soils and soil microbial activity, and to determine how variations in the extraction procedure might influence the quantity of WEOC recovered. Concentrations of WEOC were determined in soils taken from 12 different sites in the south east of Scotland, using a procedure in which samples were shaken with distilled water, centrifuged at 5000g and then filtered through 0.45 μm Millipore filters. Filtration resulted in between 30 and 400 μg C g⁻¹ being extracted using this procedure and the concentration of WEOC in the resultant extracts correlated with soil microbial production of CO₂ and dehydrogenase activity (P<0.001). Without filtration, although more WEOC was extracted (between 31 and 716 μg C g⁻¹), there was no significant correlation with biological activity. There was also no correlation between WEOC and nitrous oxide release during the incubations. Centrifugation at 20,000g for at least 10 min prior to filtration was required to remove particulate organic materials. Storage of samples at 4 °C or for up to 1 week or freezing for up to 3 months was not found to have a large influence on the concentration of WEOC in extracts, although amounts increased with soil:extractant ratio and increasing extraction time (from 15 to 60 min).     

土壤润湿性受土壤特性及土地使用的影响

Depth distribution of soil wettability and its correlations with vegetation type, soil texture, and pH were investigated under various land use (cropland, grassland, and forestland) and soil management systems. Wettability was evaluated by contact angle with the Wilhelmy plate method. Water repellency was likely to be present under permanently vegetated land, but less common on tilled agricultural land. It was mostly prevalent in the topsoil, especially in coarse-textured soils, and decreased in the subsoil. However, the depth dependency of wettability could not be derived from the investigated wide range of soils. The correlation and multiple regression analysis revealed that the wettability in repellent soils was affected more by soil organic carbon (SOC) than by soil texture and pH, whereas in wettable soils, soil texture and pH were more effective than SOC. Furthermore, the quality of SOC seemed to be more important in determining wettability than its quantity, as proofed by stronger hydrophobicity under coniferous than under deciduous forestland. Soil management had a minor effect on wettability if conventional and conservation tillage or different grazing intensities were considered.     

有机物料碳和土壤有机碳对水稻土甲烷排放的影响

基于30年水稻土长期施肥定位试验,在保证原有定位试验正常开展的前提下,将部分化肥处理变更为有机肥处理(或反之),通过观测一年水稻轮作周期内不同处理甲烷(CH_4)排放通量季节性变化,探讨不同肥力水稻土中外源有机碳及土壤有机碳含量对田间CH_4排放的影响。结果表明:施化肥处理和有机肥处理,水稻土全年CH_4累积排放量范围分别为1.73~4.72和35.09~86.60 g·m~(-2)。有机肥处理改施化肥后,田间土壤CH_4的排放量显著降低;化肥处理改施有机肥或有机肥处理增施有机肥后,田间土壤CH_4的排放量显著提高。外源有机碳的输入量是田间土壤CH_4年排放量的决定性因素,外源有机碳输入量(x)与水稻土CH_4年累积排放量(y)之间满足直线方程:y=0.087 7 x+3.265 7(R~2=0.965 9,n=21)。土壤有机碳同样也是影响稻田CH_4排放的因素,在不同有机碳水平的水稻土上施用等量相同化肥或有机肥,土壤有机碳含量高的水稻土都更有利于CH_4的产生。单施化肥稻田土壤CH_4排放的最主要碳源是土壤有机碳,有机碳含量(x)和水稻土CH_4年累积排放量(y)之间的指数方程:y=0.162 4 e~(0.162 2 x)(R~2=0.940 6,n=9)。有机肥可促进土壤有机碳分解释放CH_4,土壤有机碳含量相同的条件下,高量有机肥比常量有机肥的土壤有机碳分解比率高0.65%,等量相同有机肥但土壤有机碳含量不同的条件下,土壤有机碳分解比率无显著差异;同样,土壤有机碳也可促进有机物料碳分解释放CH_4,在常量有机肥或高量有机肥处理中,土壤有机碳含量高者比低者的有机物料碳分解比率分别多出3.57%和2.34%。     

长期免耕旱作对冬小麦生长季土壤剖面有机碳含量的影响

依托21a长期免耕秸秆还田定位试验，探究长期免耕加秸秆还田的田间管理方式对冬小麦生长季0−60cm土层内土壤有机碳（SOC）和土壤活性有机碳（MBC、POC、DOC）的影响。试验共设长期免耕秸秆还田（NT）与常规耕作（CT）两种耕作模式，分析0−60cm土层内土壤总有机碳（SOC）、土壤微生物量碳（MBC）、土壤颗粒有机碳（POC）、土壤可溶性碳（DOC）含量的变化。结果表明，在0−20cm土层，NT处理SOC含量显著高于CT处理，其中0−5cm和5−10cm土层平均SOC含量分别增加了81.2 %和52.9 %，冬小麦不同生育期内土壤SOC含量变化不显著；在0−30cm土层内，与CT处理相比，NT显著改变了土壤MBC、POC及DOC在播种前、越冬前、拔节期、开花期和成熟期5个生育阶段的分布情况，且显著提高了5个生育阶段内土壤活性有机碳的含量（P＜0.05），其中0−5cm土层内，土壤MBC、POC及DOC含量在各个时期相较于CT处理分别增长60.8%～161.4%、71.8%～141.1%和21.9%～104.4%。0−60cm土层内，两种耕作方式下的SOC、MBC、POC、DOC均随着土壤深度的增大呈下降趋势。说明长期免耕可提高耕作层土壤有机碳含量和小麦生长季活性有机碳的水平，这为旱地土壤有机碳的高效固存提供了理论依据。