Soil organic matter fractionation as a tool for predicting nitrogen mineralization in silty arable soils

After decades of searching for a practical method to estimate the N mineralization capacity of soil, there is still no consistent methodology. Indeed it is important to have practical methods to estimate soil nitrogen release for plant uptake and that should be appropriate, less time consuming, and cost effective for farmers. We fractionated soil organic matter (SOM) to assess different fractions of SOM as predictors for net N mineralization measured from repacked (disturbed) and intact (undisturbed) soil cores in 14 weeks of laboratory incubations. A soil set consisting of surface soil from 18 cereal and root‐cropped arable fields was physically fractionated into coarse and fine free particulate OM (coarse fPOM and fine fPOM), intra‐microaggregate particulate OM (iPOM) and silt and clay sized OM. The silt and clay sized OM was further chemically fractionated by oxidation with 6% NaOCl to isolate an oxidation‐resistant OM fraction, followed by extraction of mineral bound OM with 10% HF (HF‐res OM). Stepwise multiple linear regression yielded a significant relationship between the annual N mineralization (kg N/ha) from undisturbed soil and coarse fPOM N (kg N/ha), silt and clay N (kg N/ha) and its C:N ratio (R² = 0.80; P < 0.01). The relative annual N mineralization (% of soil N) from disturbed soils was related to coarse fPOM N, HF‐res OC (% of soil organic carbon) and its C:N ratio (R² = 0.83; P < 0.01). Physical fractions of SOM were thus found to be the most useful predictors for estimating the annual N mineralization rate of undisturbed soils. However, the bioavailability of physical fractions was changed due to the disturbance of soil. For disturbed soils, a presumed stable chemical SOM fraction was found to be a relevant predictor indicating that this fraction still contains bio‐available N. The latter prompted a revision in our reasoning behind selective oxidation and extraction as tools for characterizing soil organic N quality with respect to N availability. Nonetheless, the present study also underscores the potential of a combined physical and chemical fractionation procedure for isolating and quantifying N fractions which preferentially contribute to bulk soil N mineralization. The N content or C:N ratio of such fractions may be used to predict N mineralization in arable soils.     

土壤氮素矿化对烤烟产量和尼古丁含量的影响

Nitrogen （N） supply is the most important factor affecting yield and quality of flue-cured tobacco （FCT）. A field experiment and an in situ incubation method were used to study the effects of soil N mineralization in the later stages of growth on yield and nicotine content of FCT in Fenggang and Jinsha, Guizhou Province. The yield and market value of FCT at Fenggang were much lower than those at Jinsha. However, the nicotine content of middle and upper leaves was much higher at Fenggang than at Jinsha when the same rate of fertilizer N was applied, which might be due to a higher N supply capacity at the Fenggang site. At later stages of growth （7-16 weeks after transplanting）, the soil net N mineralization at Fenggang （56 kg N ha^-1） was almost double that at Jinsha （30 kg N ha^-1）. While soil NH4-N and NO3-N were almost exhausted by the plants or leached 5 weeks after transplanting, the N taken up at the later growth stages at Fenggang were mainly derived from soil N mineralization, which contributed to a high nicotine content in the upper leaves. The order of soil N contribution to N buildup in different leaves was： upper leaves 〉 middle leaves 〉 lower leaves. Thus, soil N mineralization at late growth stages was an important factor affecting N accumulation and therefore the nicotine content in the upper leaves.     

Mineralization of dissolved organic carbon in mineral soil solution of two forest soils

Dissolved organic carbon (DOC) constitutes an important carbon input flux to forested mineral soils. Seepage from mineral subsoils contains only small amounts of DOC because of mineralization, sorption or the formation of particulate organic matter (POM). However, the relation between these processes is largely unknown. Therefore, the objective of this study was to quantify the mineralization of DOC from different depths of forest soils, and to determine degradation rate constants for rapidly and slowly degradable DOC pools. Mineralization of DOC and formation of POM in mineral soil solution from two forested sites in northern Bavaria (Germany) were quantified in a 97 days laboratory incubation experiment. Furthermore, spectroscopic properties such as specific UV absorption and a humification index derived from fluorescence emission spectrometry were measured before and after incubation. DOC in all samples turned out to belong mainly to the stable DOC pool (> 95 %) with half‐lives ranging from years to decades. Spectroscopic properties were not suitable to predict the mineralization of DOC from mineral soils. However, together with data on DOC from the forest floor and long‐term data on DOC concentrations in the field they helped to identify the processes involved in C sequestration in mineral subsoils. Mineralization, formation of POM, and probably sorption seem all to be responsible for maintaining low concentrations of DOC in the upper mineral soil. DOC below the upper mineral soil is highly resistant to mineralization, and thus the further decrease of DOC concentrations in the subsoil as observed under field conditions cannot be attributed to mineralization. Our results suggest that sorption and to some minor extent the formation of POM may be responsible for C sequestration in the subsoil.     

城郊土壤不透水表面有土壤机碳转化及其相关性质的研究

Installation of impervious surface in urban area prevents the exchange of material and energy between soil and other environmental counterparts, thereby resulting in negative effects on soil function and urban environment. Soil samples were collected at 0-20 cm depth in Nanjing City, China, in which seven sites were selected for urban open soils, and fourteen sites with similar parent material were selected for the impervious-covered soils, to examine the effect of impervious surface on soil properties and microbial activities, and to determine the most important soil properties associated with soil organic carbon （SOC） transformation in the urban soils covered by impervious surfaces. Soil organic carbon and water-soluble organic carbon （WSOC） concentrations, potential carbon （C） and nitrogen （N） mineralization rates, basal respiration, and physicochemical properties with respect to C transformation were measured. Installation of impervious surface severely affected soil physicochemical properties and microbial activities, e.g., it significantly decreased total N contents, potential C mineralization and basal respiration rate （P 〈 0.01）, while increased pH, clay and Olsen-P concentrations. Soil organic carbon in the sealed soils at 0-20 cm was 2.35 kg m-2, which was significantly lower than the value of 4.52 kg m-2 in the open soils （P 〈 0.05）. Canonical correlation analysis showed WSOC played a major role in determining SOC transformation in the impervious-covered soil, and it was highly correlated with total N content and potential C mineralization rate. These findings demonstrate that installation of impervious surface in urban area, which will result in decreases of SOC and total N concentrations and soil microbial activities, has certain negative consequences for soil fertility and long-term storage of SOC.     

Quantifying and simulating carbon and nitrogen mineralization from diverse exogenous organic matters

The potential contributions of exogenous organic matters (EOMs) to soil organic C and mineral N supply depend on their C and N mineralization, which can be assessed in laboratory incubations. Such incubations are essential to calibrate decomposition models, because not all EOMs can be tested in the field. However, EOM incubations are resource-intensive. Therefore, easily measurable EOM characteristics that can be useful to predict EOM behaviour are needed. We quantified C and N mineralization during the incubation of 663 EOMs from five groups (animal manures, composts, sewage sludges, digestates and others). This represents one of the largest and diversified set of EOM incubations. The C and N mineralization varied widely between and within EOM subgroups. We simulated C and N mineralization with a simple generic decomposition model. Three calibration methods were compared. Individual EOM calibration of the model yielded good model performances, while the use of a unique parameter set per EOM subgroup decreased the model performance, and the use of two EOM characteristics to estimate model parameters gave an intermediate model performance (average RMSE-C values of 32, 99 and 65 mg C g⁻¹ added C and average RMSE-N values of 50, 126 and 110 mg N g⁻¹ added N, respectively). Because of the EOM variability, individual EOM calibration based on incubation remains the recommended method for predicting most accurately the C and N mineralization of EOMs. However, the two alternative calibration methods are sufficient for the simulation of EOMs without incubation data to obtain reasonable model performances.     

不同水分条件下添加白云石对酸性水稻土有机碳矿化的影响

施用石灰改良酸性土壤是常用的农艺措施之一。施用石灰影响土壤理化性质,进而影响土壤有机碳（Soil Organic Carbon,SOC）矿化。而SOC矿化与土壤肥力保持和有机碳库的大小存在紧密联系。因此,明晰施用石灰对酸性土壤有机碳矿化的影响具有重要的理论和现实意义。该研究以2种母质的酸性水稻土为对象,在50%、90%和130%土壤最大田间持水量（Water Holding Capacity,WHC）条件下添加和不添加白云石,再进行为期45 d的室内培养试验,探讨白云石和水分对SOC矿化的影响。研究结果表明,添加白云石显著影响2种土壤有机碳矿化速率,但白云石添加和水分的交互作用不显著。土壤含水量较低时（50% WHC）,2种土壤有机碳矿化均受到抑制。在较高土壤含水量情况下（90%～130% WHC）,白云石添加和水分的共同作用对SOC矿化的影响因土壤质地不同而异,淹水条件下（130% WHC）棕红壤有机碳矿化量高于湿润条件（90% WHC）,而红壤中的情况正好相反。白云石添加和水分均显著影响SOC累计矿化量,但二者交互作用仅在棕红壤中显著。添加白云石后,2种土壤pH值随着水分含量的增加而提高;土壤含水量较低时（50% WHC）,土壤pH值即可达到或接近目标值（pH值6.5）。这些结果表明,在评估施用白云石对SOC矿化的影响时,需要考虑土壤含水量和土壤本身的性质,以便为农业生产实践中合理施用白云石提供指导和建议。     

Soil organic carbon content and soil structure quality of clayey cropland soils: A large-scale study in the Swiss Jura region

Soil organic carbon (SOC) fashions soil structure, which is a key factor of soil fertility. Existing SOC content recommendations are based on SOC:clay ratio thresholds of >1:10. However, the corresponding SOC content might be considered hard to reach in clayey soils, whose structure degradation risk is assumed to be high. Here, we analysed the SOC content and soil structure quality of soils under similar cropping practices with clay contents ranging from 16% to 52%. Five undisturbed soil cores (5–10 cm layer) were collected from 96 fields at 58 farms in the Swiss Jura region. We assessed the soil structure quality visually using the CoreVESS method. Gravimetric air content and water content, and bulk density at −100 hPa were also measured, and the soil structure degradation index was calculated. We found that the relationship between SOC and clay content held over the clay content range, suggesting that reaching an acceptable SOC:clay ratio is not limited by large clay contents. This suggests that the 1:10 SOC:clay ratio may remain useful for clayey soils. In contrast to what was expected, it is not more challenging to reach this ratio in clayey soils even if it implies reaching very large SOC contents. SOC content explained the considered physical properties better than clay content. From a soil management point of view, these findings suggest that the soil texture determines a potential SOC content, while the SOC:clay ratio is determined by farming practices regardless of the clay content.     

Influence of soil phosphorus status and nitrogen addition on carbon mineralization from 14C-labelled glucose in pasture soils

 This study examines the effect of soil P status and N addition on the decomposition of ¹⁴C-labelled glucose to assess the consequences of reduced fertilizer inputs on the functioning of pastoral systems. A contrast in soil P fertility was obtained by selecting two hill pasture soils with different fertilizer history. At the two selected sites, representing low (LF) and high (HF) fertility status, total P concentrations were 640 and 820 mg kg^–1 and annual pasture production was 4,868 and 14,120 kg DM ha^–1 respectively. Soils were amended with ¹⁴C-labelled glucose (2,076 mg C kg^–1 soil), with and without the addition of N (207 mg kg^–1 soil), and incubated for 168 days. During incubation, the amounts of ¹⁴CO₂ respired, microbial biomass C and ¹⁴C, microbial biomass P, extractable inorganic P (P_i) and net N mineralization were determined periodically. Carbon turnover was greatly influenced by nutrient P availability. The amount of glucose-derived ¹⁴CO₂ production was high (72%) in the HF and low (67%) in the LF soil, as were microbial biomass C and P concentrations. The ¹⁴C that remained in the microbial biomass at the end of the 6-month incubation was higher in the LF soil (15%) than in the HF soil (11%). Fluctuations in P_i in the LF soil during incubation were small compared with those in HF soil, suggesting that P was cycling through microbial biomass. The concentrations of P_i were significantly greater in the HF samples throughout the incubation than in the LF samples. Net N mineralization and nitrification rates were also low in the LF soils, indicating a slow turnover of microorganisms under limited nutrient supply. Addition of N had little effect on biomass ¹⁴C and glucose utilization. This suggests that, at limiting P fertility, C turnover is retarded because microbial biomass becomes less efficient in the utilization of substrates. Received: 18 October 1999     

Carbon and nitrogen mineralization of sewage sludge compost in soils with a different initial pH

Soil properties may affect the decomposition of added organic materials and inorganic nitrogen (N) production in agricultural soils. Three soils, Potu (Pu), Sankengtzu (Sk) and Erhlin (Eh) soils, mixed with sewage sludge compost (SSC) at application rates of 0 (control), 25, 75 and 150 Mg ha⁻¹ were selected from Taiwan for incubation for 112 days. The aim of the present study was to examine the effects of SSC application rates on the carbon decomposition rate, N transformation and pH changes in three soils with different initial soil pH values (4.8–7.7). The results indicated that the highest peaks of the CO₂ evolution rate occurred after 3 days of incubation, for all treatments. The Pu soil (pH 4.8) had a relatively low rate of CO₂ evolution, total amounts of CO₂ evolution and percentage of added organic C loss, all of which resulted from inhibition of microbial activity under low pH. For the Pu and Sk soils, the concentration of NH₄⁺-N reached its peak after 7–14 days of incubation, which indicated that ammonification might have occurred in the two soils with low initial pH values. NO₃⁻-N rapidly accumulated in the first 7 days of incubation in the Eh soil (pH 7.7). The direction and extent of the soil pH changes were influenced by the N in the SSC and the initial soil pH. Ammonification of organic N in the SSC caused the soil pH to increase, whereas nitrification of mineralized N caused the soil pH to decline. Consequently, the initial soil pH greatly affected the rate of carbon decomposition, ammonification and nitrification of SSC.     

Long-term organic phosphorus mineralization in Spodosols under forests and its relation to carbon and nitrogen mineralization

In forest soils where a large fraction of total phosphorus (P) is in organic forms, soil micro-organisms play a major role in the P cycle and plant availability since they mediate organic P transformations. However, the correct assessment of organic P mineralization is usually a challenging task because mineralized P is rapidly sorbed and most mineralization fluxes are very weak. The objectives of the present work were to quantify in five forest Spodosols at soil depths of 0-15 cm net mineralization of total organic P and the resulting increase in plant available inorganic P and to verify whether net or gross P mineralization could be estimated using the C or N mineralization rates. Net mineralization of total organic P was derived from the net changes in microbial P and gross mineralization of P in dead soil organic matter. We studied very low P-sorbing soils enabling us to use lower extractants to assess the change in total inorganic P as a result of gross mineralization of P in dead soil organic matter. In addition, to enable detection of gross mineralization of P in dead soil organic matter, a long-term incubation (517 days) experiment was carried out. At the beginning of the experiment, total P contents of the soils were very low (19-51 μg g⁻¹) and were essentially present as organic P (17-44 μg g⁻¹, 85-91%) or microbial P (6-14 μg g⁻¹; 24-39%). Conversely, the initial contents of inorganic P were low (2-7 μg g⁻¹; 9-15%). The net changes in the pool size of microbial P during the 517 days of incubation (4-8 μg g⁻¹) and the amounts of P resulting from gross mineralization of dead soil organic matter (0.001-0.018 μg g⁻¹ day⁻¹; 0.4-9.5 μg g⁻¹ for the entire incubation period) were considerable compared to the initial amounts of organic P and also when compared to the initial diffusive iP fraction (<0.3 μg g⁻¹). Diffusive iP corresponds to the phosphate ions that can be transferred from the solid constituents to the soil solution under a gradient of concentration. Net mineralization of organic P induced an important increase in iP in soil solution (0.6-10 μg g⁻¹; 600-5000% increase) and lower increases in diffusive iP fractions (0.3-5 μg g⁻¹; 300-2000% increase), soil solid constituents having an extremely low reactivity relative to iP. Therefore, soil micro-organisms and organic P transformations play a major role in the bioavailability of P in these forest soils. In our study, the dead soil organic matter was defined as a recalcitrant organic fraction. Probably because gross mineralization of P from this recalcitrant organic fraction was mainly driven by the micro-organisms’ needs for energy, the rates of gross mineralization of C, N and P in the recalcitrant organic fraction were similar. Indirect estimation of gross mineralization of P in dead soil organic matter using the gross C mineralization rate seems thus an alternative method for the studied soils. However, additional studies are needed to verify this alternative method in other soils. No relationships were found between microbial P release and microbial C and N releases.     

酸雨对土壤有机碳氮潜在矿化的影响

Acid rain is a serious environmental problem worldwide. In this study, a pot experiment using forest soils planted with the seedlings of four woody species was performed with weekly treatments of pH 4.40, 4.00, 3.52, and 3.05 simulated acid rain （SAR） for 42 months compared to a control ofpH 5.00 lake water. The cumulative amounts of C and N mineralization in the five treated soils were determined after incubation at 25 ℃ for 65 d to examine the effects of SAR treatments. For all five treatments, cumulative CO2-C production ranged from 20.24 to 27.81 mg kg^-1 dry soil, net production of available N from 17.37 to 48.95 mg kg^-1 dry soil, and net production of NO3-N from 9.09 to 46.23 mg kg^-1 dry soil. SAR treatments generally enhanced the emission of CO2-C from the soils; however, SAR with pH 3.05 inhibited the emission. SAR treatments decreased the net production of available N and NO3-N. The cumulative CH4 and N2O productions from the soils increased with increasing amount of simulated acid rain. The cumulative CO2-C production and the net production of available N of the soil under Acmena acuminatissima were significantly higher （P 〈 0.05） than those under Schima superba and Cryptocarya concinna. The mineralization of soil organic C was related to the contents of soil organic C and N, but was not related to soil pH. However, the overall effect of acid rain on the storage of soil organic matter and the cycling of important nutrients depended on the amount of acid deposition and the types of forests.     

Bulk soil C to N ratio as a simple measure of net N mineralization from stabilized soil organic matter in sandy arable soils

Investigations of 23 northwestern German sandy Ap horizons (mean clay content 35 g kg⁻¹), that had higher organic matter (OM) levels than expected for sands, showed that the bulk soil C to N ratio reliably indicated the release of N from stabilized OM. Soils were incubated at 35 °C for 200 days under aerobic conditions. Cumulative N release curves were split into N released from fresh materials (N_fast) and N released from the larger pool of stabilized, older OM (N_slow rates, 0.06-0.77 μg N g⁻¹ soil d⁻¹, or 0.7-49 μg N g⁻¹ OM). Correlating the N_slow rates with total N contents of soils yielded no satisfactory relationships while their relationship with C to N ratios was very close (negative exponential, R²=0.88). Low rates of N release (N_slow) per unit of OM occurred if C to N exceeded 15. This was associated with historical factors like podzolization, calluna heathland, plaggen fertilization or a combination of these.     

施氮水平对黄土旱塬区小麦产量和土壤有机碳、氮的影响

施用氮肥是雨养农业区提高作物产量和土壤有机碳（SOC）、氮[TSN（Total soil N）]含量的重要养分管理措施。利用长期田间试验（1984～2007）,定量评价了常规耕作条件下5个施氮水平N 0（N0）、45（N45）、90（N90）、135（N135）和180（N180）kg/hm2处理下,小麦子粒产量、SOC、TSN和氮肥利用效率的变化。研究了施氮水平对黄土旱塬区旱地小麦产量、SOC和TSN积累的影响。结果表明,1984～2007年期间,N0、N45、N90、N135和N180处理小麦产量的平均值依次为1.2、2.4、2.9、3.2和3.4 t/hm2;N0处理的小麦产量随试验年限而降低,年降低幅度达67 kg/hm2（P0.001）;但增施氮肥后降低趋势得到显著控制,当施氮水平提高到N 90 kg/hm2时,产量随年限呈现出缓慢升高的趋势。随着施氮水平的提高,地上部氮肥利用率由40%（N45处理）降低到28%（N180）。不同施氮水平条件下,SOC含量随年限呈缓慢升高趋势。23年后（2007年）,N0、N45、N90、N135和N180处理下,0—20 cm土层SOC储量依次为16.9、18.2、18.7、19.0和19.1 t/hm2;TSN储量依次为2.03、2.16 、2.24 、2.34和2.37 t/hm2。施氮水平与产量呈显著的抛物线关系（R2＝0.993）。产量与SOC存在着极显著的线性相关关系（R2=0.997）。增施N 1 kg/hm2,小麦产量可提高29 kg/hm2,SOC提高1.2 kg/hm2,TSN提高0.13 kg/hm2。根茬还田量的增加是导致黄土旱塬区SOC和TSN提高的主要因素。     

Biological activity and organic matter mineralization of soils amended with biowaste composts

This study aims to elucidate the significance of compost and soil characteristics for the biological activity of compost‐amended soils. Two agricultural soils (Ap horizon, loamy arable Orthic Luvisol and Ah horizon, sandy meadow Dystric Cambisol) and a humus‐free sandy mineral substrate were amended with two biowaste composts of different maturity in a controlled microcosm system for 18 months at 5 °C and 14 °C, respectively. Compost application increased the organic matter mineralization, the C_mic : C_org ratio, and the metabolic quotients significantly in all treatments. The total amount of C_org mineralized ranged from < 1 % (control plots) to 20 % (compost amended Dystric Cambisol). Incubation at 14 °C resulted in 2.7‐ to 4‐fold higher cumulative C_org mineralization compared to 5 °C. The C_mic : C_org ratios of the compost‐amended plots declined rapidly during the first 6 months and reached a similar range as the control plots at the end of the experiment. This effect may identify the compost‐derived microbial biomass as an easily degradable C source. Decreasing mineralization rates and metabolic quotients indicated a shift from a compost‐derived to a soil‐adapted microbial community. The C_org mineralization of the compost amended soils was mainly regulated by the compost maturity and the soil texture (higher activity in the sandy textured soils). The pattern of biological activity in the compost‐amended mineral substrate did not differ markedly from that of the compost‐amended agricultural soils, showing that the turnover of compost‐derived organic matter dominated the overall decay process in each soil. However, a priming effect occurring for the Dystric Cambisol indicated, that the effect of compost application may be soil specific.     

土壤水分和植物残体对紫色水稻土有机碳矿化的影响

采用为期62.d的实验室恒温(281)℃培养方法,研究了土壤水分和植物残体对紫色水稻土有机碳矿化的影响。结果表明,紫色水稻土有机碳矿化速率在培养30.d后基本达到稳定,好气条件下土壤有机碳累积矿化量高于淹水条件,且差异达到极显著水平。用一级动力学方程对植物残体的矿化速率进行拟合表明,好气条件下,植物残体的分解速率常数(k值)大小顺序为蚕豆秸秆玉米秸秆水稻秸秆,而淹水条件则为水稻秸秆蚕豆秸秆玉米秸秆。水分状况和植物残体化学组分的差异影响紫色水稻土中有机碳的动态变化,最终导致碳累积矿化量差异。     

Soil carbon and nitrogen dynamics as affected by long-term tillage and nitrogen fertilization

Quantifying seasonal dynamics of active soil C and N pools is important for understanding how production systems can be better managed to sustain long-term soil productivity especially in warm subhumid climates. Our objectives were to determine seasonal dynamics of inorganic soil N, potential C and N mineralization, soil microbial biomass C (SMBC), and the metabolic quotient of microbial biomass in continuous corn (Zea mays L.) under conventional (CT), moldboard (MB), chisel (CH), minimum tillage (MT), and no-tillage (NT) with low (45kgNha^–1) and high (90kgNha^–1) N fertilization. An Orelia sandy clay loam (fine-loamy, mixed, hyperthermic Typic Ochraqualf) in south Texas, United States, was sampled before corn planting in February, during pollination in May, and following harvest in July. Soil inorganic N, SMBC, and potential C and N mineralization were usually highest in soils under NT, whereas these characteristics were consistently lower throughout the growing season in soils receiving MB tillage. Nitrogen fertilization had little effect on soil inorganic N, SMBC, and potential C and N mineralization. The metabolic quotient of microbial biomass exhibited seasonal patterns inverse to that of SMBC. Seasonal changes in SMBC, inorganic N, and mineralizable C and N indicated the dependence of seasonal C and N dynamics on long-term substrate availability from crop residues. Long-term reduced tillage increased soil organic matter (SOM), SMBC, inorganic N, and labile C and N pools as compared with plowed systems and may be more sustainable over the long term. Seasonal changes in active soil C and N pools were affected more by tillage than by N fertilization in this subhumid climate. Received: 20 September 1996     

Carbon mineralization in soils of different textures as affected by water-soluble organic carbon extracted from composted dairy manure

The water-soluble organic C in composted manure contains a portion of labile C which can stimulate soil microbial activity. The objective of this experiment was to evaluate the effects of water-soluble organic C extracted from composted dairy manure on C mineralization in soil with different textures. Three soils with textures varying from 3 to 54% clay were amended with 0 to 80 mg water-soluble organic C kg^–1 soil extracted from a composted dairy manure and incubated for 16 weeks at 23°C. The total amount of C mineralized was greater than the amount of C added in the three soils. Differences in mineralizable C with and without added water-soluble organic C were approximately 13–16 times, 4.8–8 times, and 7.5–8 times greater than the amount of C added to clay, loam, and sand soils, respectively. The results of this experiment suggest that immediately following composted manure applications, C mineralization rates increase, and that most of the C mineralized comes mainly from the indigenous soil organic C pool.CLBRR contribution No. 94-71     

围海造田长期耕种稻田和旱地土壤氮矿化速率及供氮潜力比较

围海造田是沿海地区拓展土地面积的主要途径。土壤氮矿化参数是揭示围海造田土壤肥力演变和土壤氮供应的重要指标,但是我国沿海造田土壤的相关研究少有报道。本研究以杭州湾南岸海积平原上慈溪市1000年和520年筑塘造田区为对象,选择4个代表性采样点,每个点从低洼稻田采集1个表层混合水稻土,在其相邻高地采集1个表层混合旱地土壤,共8个样品。采用间隙淋洗法研究了土壤样品氮矿化动力学特征。结果如下: 119 d培养试验证实水稻土和旱地土壤有机氮矿化动力学符合一级反应动力学方程Nt=N0(1-e-kt); 水稻土有机氮矿化势（N0）为82.7～161.9 mg/kg（平均114 mg/kg）,占有机氮的7.3%,旱地土壤N0为63.9～104.4 mg/kg（平均83.4 mg/kg）,占有机氮的7.3%; 水稻土有机氮矿化速率（k）为0.033～0.114/d（平均0.064/d）,旱地土壤k为0.007～0.023/d（平均0.020/d）。土壤综合供氮指标（N0k）,水稻土为3.8418.46 mg/(kgd)［平均8.0 mg/(kgd)］,旱地土壤为0.54～2.66 mg/(kgd)［平均1.6 mg/(kgd)］。水稻土总氮含量为1.4～2.0 g/kg （平均1.6 g/kg）,旱地为0.87～2.0 g/kg（平均1.3 g/kg）。可见,水稻土氮库、供氮潜力和速率均大于相邻旱地土壤。因此,从土壤氮肥力来讲,相对于旱地,围海形成的水稻田更具有可持续利用性。