贺兰山不同海拔植被下土壤团聚体分布及其稳定性研究

以贺兰山不同海拔植被下0—20,20—40 cm土层土壤为研究对象,分析不同粒径团聚体含量及团聚体稳定性随海拔升高的变化特征,探讨其与土壤理化性质之间的相关关系。结果表明:0—20 cm土层,0.25~0.053 mm团聚体为主要团聚体类型,随海拔增加,水稳性大团聚体(>0.25 mm)含量增加,<0.053 mm团聚体含量减少,表明土壤团聚体随海拔增加呈现由小粒径向大团聚体转变的趋势。20—40 cm土层,水稳性大团聚体含量在中海拔(2139 m)达到最高,占比为65.73%。平均重量直径(MWD)和几何平均直径(GMD)在0—20,20—40 cm土层均呈现随海拔升高先增加后减小的趋势,并在2139 m处达到峰值。不同海拔梯度土壤团聚体MWD和GMD与土壤有机碳(SOC)、全氮(TN)、全磷(TP)粉粒以及砂粒含量呈正相关,与黏粒含量、pH呈负相关。贺兰山不同海拔植被下土壤团聚体稳定性总体表现为中海拔>高海拔>低海拔,较高含量的大团聚体和土壤养分是团聚体稳定的关键要素。     

Effects of Organic Amendments on Soil Physical Attributes and Aggregate-Associated Phosphorus Under Long-Term Rice-Wheat Cropping

The quantification of phosphorus(P) in bulk soil and P distribution in different size fractions of water-stable aggregates(WSAs)are important for assessing potential P loss through runoff. We evaluated available and total P distribution within WSAs of a sitty clay to clay soil in a long-term fertility experiment of a rice-wheat cropping system in India. Surface soil samples were collected from seven plots amended with NPK fertilizers in combination with or without organic amendments, farmyard manure(FYM), green manure(GM), and paddy straw(PS). The plot with no NPK fertilizers or organic amendments was set as a control. The soil samples were separated by wet sieving into four soil aggregate size fractions: large macroaggregates( 2.0 mm), small macroaggregates(0.25–2.0 mm), fine microaggregates(0.05–0.25 mm), and a silt + clay-sized fraction( 0.05 mm). Structural indices were higher in the soil receiving organic amendments than in the soil receiving inorganic fertilizer alone. Organically amended soil had a higher proportion of stable macroaggregates than the control and the soil receiving inorganic fertilizer alone, which were rich in microaggregates. Total and available P contents within WSAs were inversely related to the aggregate size, irrespective of treatment. The distribution of available and total P in the soil aggregate size fraction was as follows: silt + clay-size fraction small macroaggregates fine microaggregates large macroaggregates. Within a size class, aggregate-associated available and total P contents in the organically amended soil were in the following order: FYM PS ≥ GM. The available P content of the microaggregates( 0.25 mm) was 8-to 10-times higher than that of the macroaggregates( 0.25 mm), and the total P content of the microaggregates was 4-to 5-times higher than that of the macroaggregates. Cultivation without organic amendments resulted in more microaggregates that could be checked by the application of organic amendments such as FYM and GM, which increased the proportion of water-stable macroaggregates by consolidating microaggregates into macroaggregates.     

Effect of grassland harvesting frequency and N-fertilization on stocks and dynamics of soil organic matter in the temperate climate

ABSTRACT

Management of grassland may affect the dynamics of soil organic carbon (SOC). Objectives were to analyze the effect of different harvesting frequencies and nitrogen fertilization regimes on SOC and total N stocks in a field trial on a sandy loam to loamy sand soil of a grassland site near Kiel (Germany). Additionally, effects on microbial biomass C (C_mic) and ergosterol (as proxy for fungi) contents, water-stable aggregate size-classes and density fractions were studied. In the surface soil (0–10 cm), SOC and total N stocks, amounts of large water-stable macroaggregates (> 2000 µm) and contents of C_mic and ergosterol were significantly higher under a five cut regime. C_mic (r_Spearman = 0.61) and ergosterol contents (r_Spearman = 0.67) were correlated with amounts of large water-stable macroaggregates suggesting that fungi and microbial biomass play an important role in binding of small macroaggregates into large macroaggregates. The free light fraction of SOM showed significantly higher C concentrations under three cut compared to five cut at 30–60 cm, presumably related to the C/N ratio and the decomposability of root litter. This study indicates the importance of cutting frequency on SOC and total N stocks, amounts of large macroaggregates and contents of C_mic and ergosterol.     

黑龙滩水库消落带植被恢复模式对土壤水稳性团聚体组成及其稳定性的影响

[目的] 为阐明水库消落带不同植被恢复模式对土壤水稳性团聚体组成及其稳定性的影响。[方法] 以川中丘陵区黑龙滩水库消落带人工恢复与自然恢复植被下的土壤为研究对象,利用湿筛法测定分析土壤水稳性团聚体组成特征及其稳定性指标。[结果] 消落带土壤＞0.25 mm水稳性大团聚体含量与未淹水对照相比降低4.21%,其中,自然恢复植被下土壤水稳性大团聚体含量较对照下降12.27%,人工恢复植被下大团聚体含量较对照增加3.84%;不同植被恢复模式下土壤水稳性团聚体组成差异显著(p＜0.05),人工恢复植被下土壤大团聚体含量(69.48%)显著高于自然恢复模式(43.20%);消落带土壤水稳性团聚体稳定性随水位高程增加而升高,团聚体稳定性指标＞0.25 mm水稳性团聚体含量(R_0.25)、平均重量直径(MWD)、几何平均直径(GMD)人工恢复模式下均大于自然恢复模式,团聚体分形维数(D)、土壤可蚀性因子(K)与团聚体破坏率(PAD)人工恢复模式小于自然恢复模式,表明人工恢复模式下土壤水稳性团聚体稳定性、土壤抗蚀性高于自然恢复模式。[结论] 黑龙滩水库消落带在淹水后土壤团聚体稳定性下降,但人工恢复模式能有效改善土壤团粒结构,研究结果可为黑龙滩水库消落带土壤结构稳定性评价及植被恢复提供科学依据。     

常规与有机农田土壤团聚体组成及碳氮储量研究

长期施用化肥或连作农田管理模式导致土壤质量退化及碳氮损失加剧。以常规农作大豆和转换后的有机农作大豆田土壤为研究对象,利用土壤物理分组技术,分析了土壤团聚体组成及碳氮储量变化。结果表明,常规农作大豆田转换为有机农作大豆田后,<0.053mm粉粒加黏粒比重显著降低,0.053~0.25mm较小团聚体显著增加,土壤稳定性增大,土壤及团聚体中有机碳和全氮含量都显著增加。有机农作大豆田土壤包被于较小的大团聚体(0.25~2mm)中的<0.053mm细颗粒有机质百分比显著降低,0.053~2mm粗颗粒有机质显著增加。有机农作大豆田土壤及团聚体中碳氮储量都显著高于常规农作大豆田,土壤碳汇和氮汇效应增大。有机农作大豆田土壤稳定性增加,团聚体中碳氮含量显著增加,土壤碳汇效应增强,有机农作方式可能比常规农作方式更有利于土壤碳氮资源持续利用。     

A quantification of short-term macroaggregate dynamics: influences of wheat residue input and texture

Soil structure and soil aggregation play an important role in an array of processes such as soil erodibility, organic matter protection and soil fertility. Modeling attempts of these processes would benefit substantially from including soil structural parameters such as soil aggregation. However, quantitative data on soil structural dynamics is lacking. Therefore, we conducted short-term (3 weeks) incubations to acquire necessary soil structural parameters for modeling purposes. Prior to incubation, all structures >53 μm were destroyed from three soils with varying texture but under similar management. Five different amounts of wheat residue, ranging from 0 to 3 wt%, were added to each of these soils. After 3 weeks, samples were analyzed for large water-stable macroaggregates (>2000 μm) using a wet sieving method and for fungal growth using epifluorescence microscopy. Aggregate formation increased linearly with increasing amounts of residue at a rate of 12.0±1.24 g aggregates g⁻¹ residue added. We found no differences in aggregate formation among the three soils, even though the equilibrium level of macroaggregates differed in the field. While amounts of water-stable macroaggregates in the sandy loam and the silt loam soil corresponded well with fungal lengths, this was not the case for the silty clay loam soil. This suggests that fungi are less important in aggregate formation in more clayey soils. Cumulative respiration correlated well (r=0.89-0.91) with water-stable macroaggregates for all three soils. A model assuming an aggregate formation rate proportional to the respiration rate was very successful in fitting the measured aggregate amounts. This model predicted about 65% of the changes in aggregation when different amounts were added, and about 85% of the changes in aggregation over time. This model yielded a macroaggregate turnover time of 40-60 days. The quantitative results presented here can directly be incorporated into models describing and predicting soil aggregate dynamics, as a determining factor for physical protection of organic matter within a soil.     

The capacity of soil particles for spontaneous formation of macroaggregates after a wetting-drying cycle

The capacity of soil particles for spontaneous formation of aggregates >0.25 mm was studied in a laboratory experiment. The particles from soil aggregates (3-1 mm) (initially aggregated particles, APs) and initially free particles (FPs) of <0.25 mm in size were isolated from the soddy-podzolic and chernozemic soils under fallow and from the arable soddy-podzolic soil. The aggregates of 3-1 mm were ground and passed through a 0.25-mm sieve. Then, the aggregates and free particles were poured with water and dried, and the content of the formed aggregates and their water stability were determined; in the samples from the arable soddy-podzolic soil, the organic carbon content was also determined in the newly formed aggregates. The FPs from the untilled soils formed almost no aggregates. At the same time, the APs from these soils manifested the ability for the spontaneous formation of aggregates, including water-stable aggregates. In the arable soddy-podzolic soil, on the contrary, both FPs and APs demonstrated the capacity for spontaneous self-organization into aggregates. The water stability of the self-organized aggregates from the arable soil was similar regardless of their source (APs or FPs). It was supposed that the ability of the FPs from the arable soil to form macroaggregates reflects the mechanical degradation of the aggregates in the soil: tillage results in the degradation of the aggregates, and the particles capable of spontaneously aggregation temporarily fall in the fraction of <0.25 mm. The water-stable aggregates produced from the APs or FPs of the arable soil contained more organic carbon (1.89%) in comparison with the water-stable aggregates separated from the initial 3- to 1-mm aggregates of this soil (1.31%).     

Soil organic matter in water-stable aggregates under different soil management practices in a productive vineyard

In a productive vineyard, the influence of different soil management practices on carbon sequestration and its dynamic in water-stable aggregates of Rendzin Leptosol was studied. In 2006, an experiment of different management practices in a productive vineyard was established in the locality of Nitra-Dra?ovce, in the Nitra winegrowing area of Slovakia. The following treatments were established: (1) control (grass without fertilization); (2) T (tillage); (3) T + FM (tillage + farmyard manure); (4) G + NPK3 (grass + NPK 120–55–195 kg ha^?1); and (5) G + NPK1 (grass + NPK 80–35–135 kg ha^?1). The results showed that the lowest soil organic matter content (9.70 g kg^?1) in water-stable microaggregates was determined in G + NPK3, as well as in T. However, the highest soil organic matter content in the highest size fractions of water-stable macroaggregates (>5 mm) was observed in T + FM (19.7 g kg^?1). The highest value for carbon sequestration capacity in water-stable microaggregates was observed in the ploughed farmyard manure treatment. However, the control treatment showed the highest values for carbon sequestration capacity in water-stable macroaggregates, including agronomically favourable size fractions (0.5–3 mm). In all soil management practices under a productive vineyard the most intensive changes in the soil organic matter content were observed in the highest size fractions (>3 mm) of water-stable macroaggregates.     

Myths of slash and burn on physical degradation of savannah soils: impacts on Vertisols in north Cameroon

Abstract. A study was conducted to assess the impact of agricultural land use history on macro-aggregate (4.0–4.8 mm) stability in the 0–5 cm layers of Chromic and 'Hydromorphic' Vertisols in north Cameroon. Macroaggregate stability to water drop impact was determined and the ASI₅₀ index calculated. Macroaggregates from fallow and zero-tilled cropped soils disaggregated in a stepwise manner. Macroaggregates from ploughed cropped soils collapsed in one step into semi-liquefied microaggregates and primary particles. On both soil types, the ASI₅₀ index of samples from ploughed land was 10.0 mJ, compared to 16.4– 21.9 mJ from zero-tilled slash and burn land use. The stepwise disintegration of macroaggregates indicated the existence of a hierarchy of aggregation within the size range 2–5 mm. Slash and burn land use on zero-tilled Vertisols significantly increased sand-sized organic carbon content and the stability of macroaggregates to water impact.     

连续施肥20年后棕壤团聚体分布和碳储量变化

利用两种不同分离方法(干筛法与湿筛法)对耕作施肥20年后棕壤的团聚体组成、团聚体有机碳含量以及有机碳储量进行了研究。结果表明:棕壤团聚体以0.25～1mm团聚体为主。与长期不施肥比较,除0.25～1mm粒级外长期施用氮磷化肥使风干团聚体和水稳性团聚体中较大团聚体和微团聚体数量下降,降低了各级风干团聚体中有机碳积累,增加了水稳性团聚体中有机碳积累;长期施用有机肥较大团聚体和微团聚体数量增加及其相连的有机碳含量和储量均增加;长期有机无机肥配施大团聚体数量下降,微团聚体数量增加,有机碳含量均增加,大团聚体碳库储量下降,微团聚体碳库储量增加。由此可见长期施有机肥土壤结构改善,固C潜力增加。长期高量有机肥与无机肥配施可能有利于土壤固碳,但不利于作物生长。     

土壤团聚体氧化亚氮排放潜势与微生物学机制

氧化亚氮（N2O）是主要温室气体之一,土壤是N2O的重要排放源,其排放主要受N2O产生和还原的功能微生物影响。土壤团聚体是由原生颗粒（砂、粉、黏粒）、胶结物质和孔隙组成的土壤基本结构单元。土壤不同粒径团聚体之间因基质和孔隙差异形成特殊独立的微生境被视为N2O的生物化学反应器。在不同的微生境中,N2O产生和还原的功能微生物分布不同,因而土壤不同粒径团聚体N2O排放可能存在差异。目前在不同生态系统土壤全土N2O排放特征的报道较多,而对于不同粒径土壤团聚体N2O排放相对贡献尚不清楚、功能微生物分布还未知、N2O产生和还原热区尚未明确。本文综述了近年来国内外关于土壤团聚体对N2O产生和排放机制的研究,总结了土壤团聚体性状特征对N2O产生和还原的影响,阐述了不同粒径土壤团聚体对N2O排放影响的微生物学机制,进一步明确了今后需加强土壤团聚体N2O产生和还原的热区、环境因子阈值范围的确定、系列功能基因（酶）整体性的研究,以期为N2O模拟排放模型优化提供参考,为土壤N2O减排提供理论依据。     

护坡人工混凝土壤不同粒级团聚体分布特征及其抗蚀性研究

为了定量评价人工混凝土壤生态护坡基材的抗蚀性,室内测定了其团聚体分布特征和各抗蚀性指标,并与立地条件相似的自然土壤进行了对照分析。结果表明:与自然土壤相比,人工混凝土壤中 > 0.25 mm机械稳定性团聚体含量、> 0.25 mm水稳性团聚体含量R_0.25、平均重量直径MWD、几何平均直径GWD与有机质含量均显著提升,可蚀性因子K、结构破坏率PAD、分形维数D与分散率均显著降低;同时,抗蚀性主成分值提高了2.53倍。这均证实人工混凝土壤团聚状况和团聚度有所改善,抗蚀性较自然土壤有较大的提升。相关性分析表明 > 0.25 mm水稳性大团聚体含量R_0.25和有机质含量与其他抗蚀性指标之间均存在极显著的相关性。综合分析可知,由于添加了水泥和天然有机物料,人工混凝土壤的水稳性大团聚体含量和有机质含量明显提升,因此导致其稳定性与抗蚀性显著优于原材料之一的自然土壤。     

长期保护性耕作提高土壤大团聚体含量及团聚体有机碳的作用

【目的】团聚体形成被认为是土壤固碳的最重要机制。本文以河南豫西地区长期耕作试验为研究对象,研究了长期保护性耕作对土壤团聚体性质及土壤有机碳(SOC)含量的影响,为探讨土壤固碳机理,优化黄土高原坡耕地区农田耕作管理措施,实现土壤固碳减排、培肥土壤提供理论依据。【方法】长期耕作试验开始于1999年,试验处理有免耕覆盖(NT)、深松覆盖(SM)和翻耕(CT)。利用湿筛法筛分第3年(2002年)和第13年(2011年)0—10cm和10—20 cm土层中,2000、250~2000、53~250和53μm级别的水稳性团聚体,计算团聚体平均质量直径(MWD),并测定了各级别团聚体的有机碳(SOC)含量。【结果】1)连续13年免耕覆盖和深松覆盖显著提高了土壤表层0—10 cm的SOC含量,分别比翻耕增加了33.47%和44.48%。2011年免耕覆盖和深松覆盖SOC含量较2002年上升了1.92%和8.59%,翻耕下降了18.97%。2)与翻耕相比,免耕覆盖和深松覆盖2000μm团聚体含量显著提高了40.71%和106.75%;53~250μm团聚体含量显著降低了19.72%和22.53%;团聚体平均质量直径显著提高了20.55%和39.68%,显示了土壤结构的明显改善。3)免耕覆盖和深松覆盖显著提高了表层土壤所有团聚体有机碳的含量,尤其以2000μm团聚体提升最多。与翻耕相比,2000μm团聚体有机碳分别提高了40.0%和27.6%。4)免耕覆盖和深松覆盖下表层土壤大团聚体有机碳含量随耕作年限增加,微团聚体有机碳随耕作年限降低。2000μm的土壤团聚体有机碳含量2011年较2002年分别升高了23.93%和7.12%,53~250μm微团聚体有机碳含量分别下降了19.58%和13.27%。【结论】长期保护性耕作(包括免耕覆盖和深松覆盖)可显著提高表层土壤大团聚体含量,降低微团聚体含量,提高团聚体的水稳性,改善土壤结构。同时可增加土壤团聚体有机碳含量,提高土壤肥力。长期保护性耕作在河南豫西丘陵地区是一种较为合理的耕作方式。     

宁南山区植被自然恢复中土壤团聚体特征及其与土壤性质关系

在野外调查与室内分析的基础上，对黄土丘陵区典型草原带植被自然恢复过程中土壤水稳性团聚体及其主要影响因子的演变规律进行了研究，并用典范相关分析法对二者的相互关系进行了定量分析。结果表明：随着植被演替，土壤中大粒级水稳性团聚体含量逐步增加，〉5mm粒级团聚体在土壤团粒结构中占主导地位，含量占50％～80％。其次是5～2mm含量，占到10％～15％左右。土壤全氮与有机质含量对〉5mm与1～0．5mm两个粒级团聚体影响较大。全量铝、全量铁、物理性粘粒对2～1mm与0．5～0．25mm两个粒级团聚体影响较大。全铝、全铁、全氮、有机质是影响土壤团聚体的主要因子群，其次为粘粒、物理性粘粒。主要作用因子可划分为综合物理因子（物理性粘粒，粘粒）、综合化学因子（全氮，有机质）、综合矿质因子（铁、铝氧化物）。     

改良剂对复垦土壤水稳性团聚体及POC和MOC的影响

为探究改良剂对煤矿复垦土壤有机碳的影响,以山西省襄垣县煤矿复垦区复恳7年的土壤为研究对象,采用田间微区的方法研究了泥炭和腐殖酸对复垦土壤及各粒级水稳性团聚体颗粒态有机碳(POC)和矿物结合态有机碳(MOC)的影响。结果表明:施用泥炭和腐殖酸均能增加复垦土壤水稳性大团聚体(>0.25 mm)含量,但6个月后,水稳性大团聚体会减少,而水稳性微团聚体(<0.25 mm)会增加,>2 mm和<0.25 mm粒级的团聚体变化较大,2种改良剂相比,腐殖酸各处理土壤大团聚体及微团聚体的变化比泥炭大。泥炭和腐殖酸均能增加复垦土壤总POC、MOC含量及各粒级水稳性团聚体中的POC、MOC含量,施用比例相同时,施用腐殖酸的土壤POC、MOC增量更大,施用泥炭6个月和1年后土壤POC含量变化分别为2.14~8.89,1.53~5.00 g/kg,施用腐殖酸6个月和1年后土壤POC含量变化分别为8.07~20.12,5.63~19.36 g/kg,施用泥炭6个月和1年后土壤MOC含量变化分别为4.84~10.51,5.41~8.08 g/kg,施用腐殖酸6个月和1年后土壤MOC含量变化分别为9.10~35.34,5.91~30.00 g/kg。但6个月后,泥炭和腐殖酸各处理土壤POC和MOC含量会减少。施用泥炭和腐殖酸会降低土壤有机碳的稳定性,不利于有机碳的储存。     

施肥措施对复垦土壤团聚体碳氮含量和作物产量的影响

研究复垦后不同施肥措施下有机碳（OC）和全氮（TN）在水稳性团聚体及粉黏粒组分中的分布特征,以期深入理解不同施肥措施下土壤有机碳的固持机制。以生土和连续6年不同施肥措施的复垦土壤为研究对象,采集0～20 cm耕层土壤样品,利用湿筛法进行土壤粒径分组,分析大粒径大团聚体（> 2 mm）、小粒径大团聚体（0.25～2 mm）、微团聚体（0.053～0.25 mm）和粉黏粒组分（< 0.053 mm）中OC和TN含量,判断各粒径团聚体及粉黏粒组分中有机碳储量的驱动因素,探究团聚体及粉黏粒组分中有机碳含量与作物产量之间的关系。试验设不施肥（CK）、施氮磷钾化肥（NPK）、单施有机肥（M）和有机无机肥配施（MNPK）4个处理。结果表明：1）整个试验周期（2008-2013年）,同CK相比, NPK、M以及MNPK处理均显著提高了玉米籽粒产量,且以MNPK处理的效果最显著,分别提高了79.49%、116.07%和113.85%。     

东北黑土区冻融作用对黑土水稳性团聚体的影响

Soil aggregate stability,an important index of the physical characteristics of a soil,can provide a good indication of a soil’s erodibility,and deserves special consideration in regions with cold climate.The objective of this study was to study the effect of freeze-thaw on soil water-stable aggregates in the black soil region of Northeast China.Samples of a typical black soil in the region were collected to measure water-stable aggregates after freeze-thaw under different conditions(i.e.,initial moisture contents,freezethaw cycles and freezing temperatures)by wet-sieving into eight particle size groups(10,10–6,6–5,5–3,3–2,2–1,1–0.5,and0.5–0.25 mm).Freeze-thaw had the most effect on aggregate stability when the samples had an initial moisture content of 400 g kg-1.The water-stable aggregates of the four larger particle size groups(5,5–3,3–2,and 2–1 mm)reached a peak stability value,but those of the two smaller particle size groups(1–0.5 and 0.5–0.25 mm)reached a minimum value when the soil moisture content was 400 g kg-1.Water-stable aggregates of the four larger particle size groups decreased while those of the two smaller particle size groups increased with the increase of freeze-thaw cycles.As temperatures fell,the water-stable aggregates of the four larger particle size groups decreased while those of the two smaller particle size groups increased.     

套作及秸秆还田对西兰花连作田土壤团聚体分布的影响

随着蔬菜的规模化与集约化发展,菜田连作障碍日趋严重,合理间套作与秸秆还田对恢复菜田土壤生态环境有很大的潜力。该文基于6a的田间长期定位试验,以西兰花单作为对照,设置西兰花套作糯玉米且糯玉米秸秆还田-西兰花(B/MR-B)、西兰花套作糯玉米且糯玉米秸秆不还田-西兰花(B/M-B)、西兰花-西兰花(B-B),共3个处理。采用湿筛法探索了套作糯玉米(Zea mays L.sinensis Kulesh)并秸秆还田对连作西兰花(Broccoli)田土壤固碳能力以及土壤水稳定性团聚体分布的影响。结果表明:随着土层的增加,不同处理的水稳定团聚体分布呈粒径逐渐减小、分布范围呈逐渐集中趋势。随着生育时期的推进,幼苗期和花球生长期水稳性团聚体多集中于0.25~0.5 mm与0.25 mm粒径范围;营养生长期多集中于0.5~1 mm与0.25~0.5 mm粒径范围;而秋菜收获期则多分布于0.5~1 mm、0.25~0.5 mm与0.25 mm粒径范围。B-B处理下各个时期0~40 cm土层的粒径0.5~1 mm和0.25~0.5 mm的水稳性团聚体基本呈现低于其他处理,相反B-B处理下的粒径0.25 mm水稳性团聚体均呈现显著高于其他处理。秋季西兰花整个生育期水稳定性大团聚体均表现为B/MR-BB/M-BB-B。B/MR-B处理的土壤有机碳(SOC)含量显著高于其他处理。多元回归分析表明土壤水稳定性大团聚体与土壤有机碳含量在0~10 cm与10~20 cm土层中呈极显著(P0.01)正相关,20~40 cm土层呈显著(P0.05)正相关。综上,套作糯玉米并秸秆还田能有效提高连作西兰花田耕作层土壤水稳定性大团聚体的比例,改善连作导致的土壤理化性状的恶化。     

连续失水-复水中不同粒径保水剂对土壤结构和水分特性的影响

为明确土壤干湿交替环境下保水剂与土壤颗粒之间的作用方式及保水剂对土壤结构和水分有效性的影响。通过土柱模拟试验,研究连续失水—复水条件下,不同粒径(0.85,0.6～0.85,0.30～0.45 mm)保水剂对土壤水分特性、土壤团聚体分布和结构稳定性的作用,以及保水剂—土壤混合体价键结构的微观变化特征。结果表明:第1次(T1)和第2次(T2)失水取样阶段,除T2阶段的最小粒径处理外,其余各粒径保水剂促使土壤相对含水量较对照显著增加72.9%以上(P0.05)。保水剂粒径大小和土壤水分状况均会影响保水剂对土壤供水能力的作用效果,粒径最小的保水剂改变土壤持续吸水和供水的能力较差,促进土壤水稳性大团聚体组成和团聚结构稳定的性能最优。在连续的干湿交替影响下,保水剂与土壤的作用加剧,壤土中的Si-O-Si键、-OH、蒙脱石和石英等矿物胶体会进入到保水剂网状结构中,反应的剧烈程度会影响土壤水稳性大团聚体的形成和稳定性。初步分析认为,随着反复失水—复水进程,更多黏粒矿物对保水剂分子结构的破坏,是减弱保水剂吸持水性能的主要因素之一。     

长期种植毛竹林土壤丛枝菌根真菌群落演变趋势

为揭示长期种植毛竹林土壤丛枝菌根(Arbuscular mycorrhizae,AM)真菌群落演变特征,通过磷脂脂肪酸(Phospholipid fatty acid,PLFA)和Illumina Miseq测序平台研究了AM真菌生物量及群落结构的演变趋势。结果表明,长期种植毛竹林土壤养分含量总体呈下降趋势,球囊霉素相关土壤蛋白(Glomalin-related soil protein,GRSP)含量及AM真菌生物量也显著降低(p0.05),其中易提取态球囊霉素相关土壤蛋白(Easily extractable glomalin-related soil protein,EE-GRSP)含量与有机质、碱解氮、速效钾显著正相关(p0.05),而AM真菌菌丝生物量(16:1ω5 PLFA)与碱解氮极显著正相关(p0.01)。长期种植毛竹林显著降低了土壤2~0.25 mm大团聚体比例(p0.05),且与AM真菌菌丝生物量极显著正相关(p0.01)。测序结果表明,毛竹林土壤AM真菌以球囊霉属(Glomus)为优势种群,其次为无梗囊霉属(Acaulospora),长期种植毛竹后土壤球囊霉属相对丰度显著增加而无梗囊霉属显著降低(p0.05)。非度量多维尺度转换排序(Non-metric multidimensional scaling,NMDS)分析显示,对照马尾松林与不同种植年限毛竹林土壤AM真菌群落显著区分(p=0.001),土壤含水量(p=0.005)、碱解氮(p=0.001)、有效磷(p=0.014)对AM真菌群落结构变异具有重要贡献。长期种植毛竹显著降低了AM真菌生物量、球囊霉素相关土壤蛋白含量以及2~0.25 mm大团聚体比例,并改变了AM真菌群落结构,不利于土壤碳固存和维持生态系统稳定。