Saltiness enhancement by taste contrast in bread prepared with encapsulated salt

In this study, we investigate a technological approach to reduce the sodium content of bread whilst retaining its sensory profile by creating taste contrast using encapsulated salt. We demonstrate that sensory contrast in bread induced by encapsulated salt can enhance saltiness and allows for a salt reduction of up to 50% while maintaining saltiness intensity. The magnitude of the saltiness enhancement depends on the size of the salt encapsulates. Small encapsulates lead to small concentration gradients of salt which do not affect saltiness intensity and consumer liking. Large encapsulates lead to large concentration gradients which enhance saltiness intensity significantly and reduce consumer liking. To achieve maximum salt reduction while maintaining saltiness and liking of the bread, the dimensions of the encapsulated salt particles need to be optimized. Taste contrast is a technology to modulate taste perception and to create sodium reduced products while retaining their saltiness intensity and consumer liking.     

The effect of infrared stabilized rice bran substitution on physicochemical and sensory properties of pan breads: Part I

Infrared stabilized rice bran (SRB) substitution to white wheat, wheat bran and whole grain wheat breads at the levels of 2.5, 5.0 and 10.0% was evaluated in terms of proximate composition, crumb color, dietary fiber, texture and sensory attributes. An increasing tendency was observed in crude fat and ash content of the breads. Redness (+a*), yellowness (+b*) chroma and redness (a*/b*) values were increased gradually with the addition of SRB. Crumb color was found to be darker when 10% of SRB was added to the breads. SRB inclusion did not affect the content of soluble dietary fiber while it significantly increased the insoluble and total dietary fiber contents of the breads (p < 0.05). In general, whole grain wheat bread differed from the other bread types in terms of textural behavior. Based on the overall acceptability scores, white wheat and wheat bran breads were sensory accepted up to 10% of flour replacement with rice bran, while substitution levels higher than 2.5% negatively affected the sensory scores of whole grain wheat bread.     

A Multistrategic Approach in the Development of Sourdough Bread Targeted Towards Blood Pressure Reduction

Rising prevalence of hypertension is pushing food industry towards the development of innovative food products with antihypertensive effects. The aim was to study the effect of reduced sodium content and 21 % addition of wholemeal wheat sourdough (produced by Lactobacillus brevis CECT 8183 and protease) on proximate composition, γ-aminobutyric acid (GABA) and peptide content of wheat bread. Angiotensin converting enzyme I (ACE) inhibitory and antioxidant activities were also evaluated. Sodium replacement by potassium salt did not affect chemical composition and biological activities of bread. In contrast, GABA and peptides <3 kDa contents in sourdough bread (SDB) were 7 and 3 times higher, respectively, than the observed in control. ACE inhibitory and antioxidant activities of the peptide fraction?<?3 kDa from SDB was 1.7 and 2.6-3.0 times higher than control. Therefore, the combination of reduced sodium content with enriched concentrations of bioactive compounds in bread making may provide interesting perspectives for development of innovative breads towards blood pressure reduction.

     

Folates Stability in Two Types of Rye Breads During Processing and Frozen Storage

High-performance liquid chromatography was used to study the stability of folate vitamers in two types of rye breads after baking and 16 weeks of frozen storage. Bread made using sourdough seeds contained less total folate (74.6 μg/100 g dry basis, expressed as folic acid) than the whole rye flour (79.8 μg/100 g dry basis) and bread leavened only with baker’s yeast (82.8 μg/100 g dry basis). Most importantly, it was generated by a significant decrease in 5-CH₃-H₄folate form. The baking process caused some changes in folate distribution. Storage of breads at -18°C for 2 weeks did not have a significant effect (p < 0.05) on total folates compared to the content directly after baking. After a 5-weeks storage period, a significant decrease (p < 0.05) in the content of total folates was recorded and it dropped on average by 14% for both type of breads. After a longer period of storage (16 weeks), a 25% loss of folates in the bread made with baker’s yeast and a 38% loss in the bread fermented with sourdough seeds was found. Retention of 5-CH₃-H₄folate and 10-HCO-H₂folate forms were much lower in the bread made with a sourdough addition than with baker’s yeast only.     

Sourdough fermentation of wholemeal wheat bread increases solubility of arabinoxylan and protein and decreases postprandial glucose and insulin responses

Glycemic responses to most of the conventional breads are high, including breads made of wholemeal flour. Baking technology is known to affect these responses. The aim of the present study was to investigate effects of xylanase enzyme treatment and sourdough fermentation in wholemeal wheat bread baking on postprandial glucose and insulin responses and on in vitro protein digestibility. The wheat breads were made of 100% flour from peeled kernels by a straight dough or sourdough fermentation method, and with or without using xylanase during mixing of dough. Standard white wheat bread was used as a reference. All test bread portions contained 50 g available carbohydrate and were served in random order to eleven insulin resistant subjects. Blood samples for measuring glucose and insulin concentrations were drawn over 4 h. The sourdough wholemeal wheat bread resulted in the lowest postprandial glucose and insulin responses among the four tested breads (treatment × time; p = 0.000 and p = 0.022, respectively). There were differences in solubility and depolymerisation of protein and arabinoxylan among the breads but these did not fully explain the in vivo findings. In conclusion, the health effects of wholemeal wheat bread can be further improved by using sourdough process in breadmaking.     

Improving the quality of nutrient-rich Teff (Eragrostis tef) breads by combination of enzymes in straight dough and sourdough breadmaking

The growing interest in the benefits of wholegrain products has resulted in the development of baked products incorporating less utilised and ancient grains such as, millet, quinoa, sorghum and teff. However, addition of wholegrains can have detrimental effects on textural and sensory bread product qualities.Enzymes can be utilised to improve breadmaking performance of wholegrain flours, which do not possess the same visco-elastic properties as refined wheat flour, in order to produce a healthy and consumer acceptable cereal product.The effects of Teff grain on dough and bread quality, selected nutritional properties and the impact of enzymes on physical, textural and sensory properties of straight dough and sourdough Teff breads were investigated.Teff breads were prepared with the replacement of white wheat flour with Teff flour at various levels (0%, 10%, 20%, and 30%) using straight dough and sourdough breadmaking. Different combinations of enzymes, including xylanase and amylase (X + A), amylase and glucose oxidase (A + GO), glucose oxidase and xylanase (GO + X), lipase and amylase (L + A) were used to improve the quality of the highest level Teff breads. A number of physical, textural and sensory properties of the finished products were studied. The nutritional value of breads was determined by measuring chemical composition for iron, total antioxidant capacity, protein, fibre and fat. The obtained results were used to estimates intakes of nutrients and to compare them with DRIs.The incorporation of Teff significantly (P < 0.05) improved dietary iron levels as 30% Teff breads contained more than double the amount of iron when compared to corresponding wheat bread (6 mg/100 g v 2 mg/100 g). Addition of Teff also significantly (P < 0.05) improved total antioxidant capacity from 1.4 mM TEAC/100 g to 2.4 mM TEAC/100 g. It was estimated that an average daily allowance of 200 g of Teff enriched bread would contribute to DRIs in the range of 42-81% for iron in females, 72-138% for iron in males; 38-39% for protein in males, 46-48% for protein in females; and 47-50% of fibre in adults.The major challenge was encountered in producing the highest level of Teff bread with good textural and sensory attributes. Increasing the level of Teff significantly (P < 0.05) increased dough development time, degree of softening, crumb firmness and bitter flavour whilst decreasing the dough stability, specific loaf volume and overall acceptability of the bread. Teff breads produced with the addition of enzyme combinations showed significant improvements (P < 0.05) in terms of loaf volume, crumb firmness, crumb structure, flavour and overall acceptability in both straight dough and sourdough breadmaking.     

Effects of pressure treatment of hydrated oat,finger millet and sorghum flours on the quality and nutritional properties of composite wheat breads

Breadmaking achievement using grains alternative to wheat and rye is a challenging task for cereal technologists, since most of the available innovative breads are characterised by poor crumb and crust characteristics, slight flavour and fast staling. To improve texture, mouth-feel, acceptability and shelf-life of breads prepared by using minor and/or under-utilised cereals, gluten and/or polymeric substances that mimic the viscoelastic properties of gluten, are required. Recent studies reported that high hydrostatic pressure (HP) treatment may represent an efficient non-thermal technique to promote the dough structure formation of composite cereal matrices. In the present study the effects of HP on the techno-functional and nutritional properties of oat-, millet-, and sorghum- based breads were evaluated compared to their unpressured- and gluten-added conventionally made counterparts. HP-treated (350 MPa, 10 min) wheat, oat, millet and sorghum batters were added to the bread recipe, replacing 50%, 60% and 40% of untreated wheat flour, respectively. Data from bread analyses revealed non significant physico-chemical impairment, and superior nutritional and sensory profiles in most quality features when HP treatment was applied to dough batters, compared with conventional/gluten-added samples. Specifically, HP breads deserved better sensory scores and exhibited higher antiradical activities despite a reduction in specific volume (wheat and oat) and faster staling kinetics (millet and sorghum) that were explicit in some composite samples.     

It’s a maze: The pore structure of bread crumbs

Bread has a foam-like structure, but to date there is little information about the morphology of void structure in breads in three dimensions. In this paper, permeability testing and analysis of computer aided micro-tomography data were used to investigate the structure of breads as a function of connectivity of voids in a range of breads. The findings were related to bread properties obtained via material testing. Results indicated that a single, massively interconnected, open cell was responsible for approximately 99% of bread’s total porosity. Closed pores are also present with the number and average volume of closed pores significantly affecting strengths of bread crumbs. Bread behaved similarly to foams described by Gibson and Ashby (1988). The role of open and closed pores in affecting bread toughness and texture requires further investigation via structural–mechanical models.     

Quality and microbial characteristics of part-baked Sangak bread packaged in modified atmosphere during storage

The effect of part-baking and modified atmosphere packaging (MAP) on quality and microbial stability of part-baked flat bread (Sangak) was investigated. Part-baked Sangak bread was packaged in 100% CO₂, 50% CO₂:50% N₂, 25% CO₂:75% N₂ and air as a control and stored at 25 °C for 21 days. Color, firmness, moisture, density and microbiological analyses of the package breads were carried out at 3-day intervals during storage. No significant effect of MAP was found on moisture, color and texture during the storage of part-baked bread, while the total aerobic plate count (APC) and the mold and yeast count (M + Y) were dependent on the concentration of CO₂ in the package headspace. With increasing concentration of CO₂, microbial counts were decreased. Density of part-baked bread was also somewhat influenced by the MAP. The observed reduction in the density of control samples during storage was less than those in MAP. The results of the present study demonstrated that it is possible to prolong the shelf-life of Sangak bread from a few days to about 21 days by using bake-off technology (BOT) and MAP under high CO₂ concentration.     

Multi-elemental analysis of flour types and breads by using laser induced breakdown spectroscopy

Bread and flour are most commonly used products in human diet, which makes it susceptible to adulteration, mislabeling and addition of unpermitted amount of different flour types. The objective of this work was to evaluate the potential of employing laser induced breakdown spectroscopy to differentiate different flour types and quantify the white wheat flour addition to rye and oat flour and breads. In the principal component analysis, score plot represents pure flour types with 97.64% of the variance. In the calibration study, the measured coefficient of determination values was 0.989, 0.989, 0.992 and 0.991 for refined wheat flour: rye flour, refined wheat flour: oat flour, breads made with the blend of refined wheat: rye flour and the blend of refined wheat: oat flour, respectively. The limit of detection values were calculated as 3.82, 5.97, 4.59 and 4.92% for refined wheat flour: rye flour, refined wheat flour: oat flour, refined wheat: rye bread and refined wheat: oat bread, respectively.     

Stability of B-complex vitamins and dietary fiber during rye sourdough bread production

The stability of vitamers: thiamine, riboflavin, nicotinic acid, nicotinamide, pantothenic acid, pyridoxine and pyridoxal, as well as soluble and insoluble dietary fiber was studied in a rye sourdough bread process. The vitamer concentrations were measured in raw materials (rye flours, white and red rye malt, yeast) and the rye sourdough breads made from them by means of LC–MS and stable isotope dilution assay. The content of dietary fiber was determined using a standard enzymatic-gravimetric method. During baking, the concentration of vitamins decreased by 20–45% in the case of thiamine, 25–50% in the case of nicotinic acid, 45–65% in the case of pyridoxal in both breads, 50% in the case of riboflavin and 15% in the case of pyridoxine only in fine rye bread. In contrast, the content of nicotinamide increased during processing by ten fold, presumably due to microbial activity during sourdough fermentation. The ratio of soluble to insoluble dietary fiber increased during rye sourdough processing.     

Impact of baking conditions and storage temperature on staling of fully and part-baked Sangak bread

Bread staling involves a combination of physico-chemical phenomena that leads to a reduction of quality. This study aims at evaluating the impact of baking conditions (280 °C, 8 min; 310 °C, 5.5 min; 340 °C, 4 min), baking type (of fully baked (FB) and part-baked (PB)) and storage temperature (−18, 4 and 20 °C) on the staling of Sangak bread. Results showed that lower baking temperature with longer baking time produced drier bread with higher firmness. In FB Sangak breads, amylopectin retrogradation, amount of unfreezable water and firmness (measured by compression test) increased during storage at positive temperatures but hardness (determined by Kramer shear test) decreased significantly during first day of storage. The recrystallized amylopectin traps the free water resulting in crumb hardening. Water is also absorbed by the dry crust resulting in changes of rheological properties in the crust and crumb, and finally in staling. Storage at 4 °C resulted in increasing melting enthalpy of amylopectin crystallite in comparison with storage at 20 °C. Also it was found that firmness of PB breads due to rebaking was significantly lower than FB breads. There were no significant changes in staling parameters of FB and PB stored at −18 °C.     

Glucose and insulin responses in healthy men to barley bread with different levels of (1→3;1→4)-β-glucans; predictions using fluidity measurements of in vitro enzyme digests

Bread products with three different levels (35, 50 and 75%) of the (1→3;1→4)-β-glucan rich barley genotype Prowashonupana (PW), 50% common barley (CB) or 100% white wheat, were given as a breakfast meal to 10 men and their postprandial blood glucose and insulin responses were measured. In addition, the viscosity of (1→3;1→4)-β-glucans isolated from the pw flours was measured, and the fluidity characteristics of the (1→3;1→4)-β-glucans in the bread products estimated in in vitro enzymatic digests prepared under conditions simulating those prevailing in the gastrointestinal tract. Bread containing 50 and 75% PW flour lowered the glycaemic index (GI) (40 and 48%, respectively) and insulinaemic index (II) (37 and 34%) compared with a white wheat reference bread (GI=100; II=100). A high correlation (r=0.9782; P=0.0007) was found between the fluidity index (FI) of the enzymatic digests and GI of all bread products (GI=50.8+0.441FI). The overall conclusions were that incorporation of (1→3;1→4)-β-glucan rich barley in bread may lower its glycaemic and insulinaemic properties and that the metabolic response could be predicted by measuring the in vitro fluidity of bread digests.     

Factors influencing acrylamide formation in rye,wheat and spelt breads

In this study, a simple strategy for acrylamide (AA) reduction in white and dark wheat and spelt and rye breads, including the impact of flour basic composition, flour extraction rate, type of technology and baking time and temperature was addressed. Moreover, the correlation between AA formation in breads and total phenolic compounds (TPC) and antioxidant capacity (AC) of flours and breads was calculated. The studies showed an impact of flours origin on AA formation in breads with the following rank: wheat bread ≤ spelt bread < rye bread. There was no statistically significant effect of flour extraction rates and their chemical components on AA formation in breads baked at 200 °C/35 min. However, a weak effect was noted for wheat and spelt breads baked at higher temperature. In contrast, a positive correlation between AA in wheat, spelt and rye breads baked at both applied conditions (200 °C for 35 min or 240 °C for 30 min) and AC of white and dark flours was noted. The same finding was noted between AA formation and TPC and AC of bread and its crust. The provided data indicated that AA mitigation strategy should be based on the selection of lower baking temperature with longer baking time as the main important factor amongst others.     

Permeability of crust is key to crispness retention

Bread loses crispness rapidly after baking because water originating from the wet crumb accumulates in the dry crust. This water accumulation might be increased by the dense and low permeable character of the bread crust. Our objective was to investigate the influence of permeability of the crust on water uptake in the crust and on crispness retention. To achieve this objective, we increased the permeability of the control bread crust by creating small channels through the crust. The water vapour permeability of the crust with and without channels was measured using a newly developed method for brittle materials. Two further properties were measured over time: crispness of bread by analysing acoustic properties and water content of the crust. Control bread crust had low water vapour permeability and functioned as a barrier, leading to increased uptake of water in the crust. Water uptake was halved, however, if the water vapour permeability of the crust was doubled. As a consequence, crispness retention increased eight-fold; breads stored for four hours were as crispy as control breads stored for 30 min. We can conclude, therefore, that permeability of crust is key to crispness retention.     

Suitability of tef varieties in mixed wheat flour bread matrices: A physico-chemical and nutritional approach

Wheat flour replacement from 0 to 40% by single tef flours from three Ethiopian varieties DZ-01-99 (brown grain tef), DZ-Cr-37 (white grain tef) and DZ-Cr-387 (Quncho, white grain tef) yielded a technologically viable ciabatta type composite bread with acceptable sensory properties and enhanced nutritional value, as compared to 100% refined wheat flour. Incorporation of tef flour from 30% to 40% imparted discreet negative effects in terms of decreased loaf volume and crumb resilience, and increase of crumb hardness in brown tef blended breads. Increment of crumb hardness on aging was in general much lower in tef blended breads compared to wheat bread counterparts, revealing slower firming kinetics, especially for brown tef blended breads. Blended breads with 40% white tef exhibited similar extent and variable rate of retrogradation kinetics along storage, while brown tef-blended breads retrograded slower but in higher extent than control wheat flour breads. Breads that contains 40% tef grain flour were found to contain five folds (DZ-01-99, DZ-Cr-387) to 10 folds (DZ-Cr-37) Fe, three folds Mn, twice Cu, Zn and Mg, and 1.5 times Ca, K, and P contents as compared to the contents found in 100% refined wheat grain flour breads. In addition, suitable dietary trends for lower rapidly digestible starch and starch digestion rate index were met from tef grain flour fortified breads.     

Texturized Pinto Bean Protein Fortification in Straight Dough Bread Formulation

Pinto beans were milled and then air-classified to obtain a raw high protein fraction (RHPF) followed by extrusion to texturize the protein fraction. The texturized high protein fraction (THPF) was then milled to obtain flour, and combined with wheat flour at 5, 10, and 15 % levels to make bread. The air-classification process produced flour with high concentration of lipids and phytic acid in the protein-rich fraction. However, extrusion significantly reduced hexane extractable lipid and phytic acid. However, the reduction observed may simply indicate a reduction in recovery due to bind with other components. Total protein and lysine contents in composite flours increased significantly as THPF levels increased in composite flour. Bread made with 5 % THPF had 48 % more lysine than the 100 % wheat flour (control). The THPF helped to maintain dough strength by reducing mixing tolerance index (MTI), maintaining dough stability and increasing departure time on Farinograph. Bread loaf volume was significantly reduced above 5 % THPF addition. THPF increased water absorption causing an increase in bread weights by up to 6 %. Overall, loaf quality deteriorated at 10 and 15 % THPF levels while bread with 5 % THPF was not significantly different from the control. These results support the addition of 5 % THPF as a means to enhance lysine content of white pan bread.     

Determination of visual detection thresholds of selected iron fortificants and formulation of iron-fortified pocket-type flat bread

Wheaten pocket-type flat breads were baked from refined flours fortified to contain iron from ferrous sulphate (FeSO₄), hydrogen-reduced elemental iron (Fe) or sodium iron ethylenediaminetetraacetic acid (NaFeEDTA). Individual and group visual detection thresholds were determined by the 3-alternative forced choice (3-AFC) test according to the American Society of Testing and Materials (ASTM) method E-1432. The group visual detection thresholds of FeSO₄, NaFeEDTA and Fe in bread were established in samples baked from the corresponding wheat flours fortified with 69.46, 236.82 and 304.97 mg iron/kg flour, respectively. Sensory testing showed that iron-fortified pocket-type flat breads were similar (P < 0.01) to regular bread when baked from flours formulated to contain iron at levels lower by 25% than the group visual detection thresholds of Fe and FeSO₄ and two 25% increments lower than the threshold of NaFeEDTA. These findings indicate that iron-fortified pocket-type flat breads, which are sensorially similar to regular bread, baked from flours that contained 52.1, 133.22 and 228.73 mg iron/kg flour as FeSO₄, NaFeEDTA and Fe, would provide the segment of the population at the highest risk of iron-deficiency anaemia, specifically women of childbearing age, with 91%, 207% and 346% of their recommended daily intakes for iron, respectively.     

Effect of arabinoxylans and laccase on batter rheology and quality of yeast-leavened gluten-free breads

The supplementation effects of maize fiber arabinoxylans (MFAX, 0%–6%), laccase (0–2 U/g flour) and water absorption level (90%–100%) on gluten-free (GF) batter rheology and bread quality were analyzed. From viscoamylograph analysis, lower starch amount in GF flour due to MFAX addition decreased peak viscosity and retrogradation. Surface response plots showed that laccase did not have significant effect on GF batter rheology and bread quality, whilst water was the most important variable. Higher levels of water absorption benefited bread texture. Higher water level (>100 mL/100 g flour) was needed in the experimental design to evaluate correctly the effect of 6% MFAX replacement on GF bread quality. Further analyses were carried in order to adjust water absorption of batters according to their consistency index (K ≈ 100 Pa sⁿ), resulting an optimal water absorptions of 95%, 100% and 105% for control flour and flours supplemented with 3% or 6% MFAX, respectively. Thus, MFAX addition enhanced water-binding capacity of flour and yielded GF breads with higher specific volume and softer crumb texture. These quality parameters were best rated with 6% MFAX addition to flours. This research demonstrated the potential of MFAX to develop GF breads with improved quality, when optimal water level is used.     

High (1→3,1→4)-β-Glucan Barley Fractions in Bread Making and their Effects on Human Glycemic Response

Barley flour (BF) from whole naked grain and two (1→3,1→4)-β-glucan-enriched fractions, a sieved fraction (SF) and a water-extracted fraction (WF), were produced and mixed with bread wheat flour (BW), for bread-making quality evaluation. Bread was baked in a pilot plant and analysed for sensory properties, proximate composition and (1→3,1→4)-β-glucan content. Four kinds of bread were produced from different mixtures of barley ingredients and bread wheat flour: a 100% BW ((1→3,1→4)-β-glucan content: total, 0·1%; soluble, 0·1%); a 50% BW and 50% BF (2·4%; 2·0%); a 50% BW and 50% SF (4·2%; 2·8%); an 80% BW and 20% WF (6·3%; 5·7%). Eight adults were fed test meals of each of the four breads, containing the same amount (50 g) of available carbohydrate, and glycemic indices calculated from finger-prick capillary blood samples. A linear decrease in glycemic index was found for increasing (1→3,1→4)-β-glucan content. The 20% WF bread had a 28% lower glycemic index than the plain BW and also showed the best scores for sensory attributes. This research confirms the effectiveness of viscous (1→3,1→4)-β-glucan in reducing postprandial blood glucose levels, even in foods with a high glycemic index. The enrichment technique, and water extraction/freeze-drying technique, could enable the use of barley as a source of a high-value fibre for reducing the glycemic index of traditional wheat-based foods such as bread, without negatively affecting their sensory characteristics.