猪圆环病毒病及其防制

猪圆环病毒Porcine circovirus，PCV)是迄今发现的一种最小的动物病毒。现已知PCV有两个血清型，即PCV1和PCV2。PCV1为非致病性的病毒。PCV2为致病性的病毒，它是断奶仔猪多系统衰竭综合征(Postweaning Muhisystemic Wasting Syndrome．PMWS)的主要病原。本病最早发现于加拿大(1991)，     

猪圆环病毒病

<正> 定义:本病是由Ⅱ型圆环病毒所引起的各种日龄的猪均可感染和发病的,临床上以仔猪先天性震颤、断奶仔猪衰竭及皮炎为主要特征的一种传染病。流行趋势:本病多发生养殖比较集的地区和国家,最早在我国报道是在2000-2001年,主要集中广东、广西、福建等省份,从2003年开始在我国各省份发生和流行,目前已给各地养殖业带来非常严重的损失。流行病学1、本病多与细小病毒病、蓝耳病、猪瘟、气喘病等混合感染。2、可通过各种途径传播如:消化道、呼吸道等,尤其消化道是本病传播的主要途径。3、目前本病在我国呈流行性发病,故望广大养殖户加以重视。     

猪圆环病毒病

前　　言近年来 ,一种新的病毒和一种新的病毒病在世界上许多国家流行 ,那就是猪圆环病毒和猪圆环病毒病。1997年 ,Ｃａｒｌｔｏｎ在《猪兽医师》(《ＳｗｉｎｅＰｒａｃｔｉｔｉｏｎｅｒ》)杂志上 ,第一次提出了猪断奶后多系统衰竭综合征(ＰＭＷＳ)的概念 ,随后ＰＭＷＳ一词被研究者广泛接受和采用。ＰＭＷＳ由一种被称为猪圆环病毒 (ＰＣＶ)的病毒引起。实际上 ,猪圆环病毒除了主要是ＰＭＷＳ的病原以外 ,还与猪的其他疾病有关。目前经研究证明 ,猪圆环病毒还与猪的繁殖障碍、猪的间质性肺炎和猪的皮炎和肾病综合征有关。目前这些疾病已造…     

猪圆环病毒病研究进展

圆环病毒是近年来兽医界广泛关注的新发现畜禽类病毒之一,具有重要的经济意义.在2000年墨尔本国际猪病会议(IPVS)和1999年北京国际禽病会议(ICEVP)上均成为受到重视的热门话题.     

金华地区猪圆环病毒病病原流行病学调查

试验从2008年12月至2010年3月期间采集金华地区部分猪场疑似PCV2感染症状病料102份,采用PCR技术,利用所设计合成的一对PCV2型特异性引物,对病料中的PCV2目的片段进行扩增检测,试验结果显示平均阳性率为59.80%;然后对PCV2阳性病料进行混合感染检测,结果显示病料中一定程度地感染了PRV、PRRSV以及PPV,且混合感染比单纯感染占更大比例,其中二重感染以PCV2与PRRSV混合感染最高;三重感染以PCV2、PRRSV和PRV三者感染最高.     

简介猪圆环病毒病

在我国,郎洪武等采集来自北京,河北,山东,天津、江西、吉林,河南等7省市22个猪群的559份血清,用ELISA方法检测,结果20日龄未断奶仔猪、1～2月龄断奶仔猪,后备母猪和经产母猪的总阳性率为42．9％,表明以PCV-2病原为主而导致的PMWS病在我国较为普遍。     

猪圆环病毒病

1猪圆环病毒病的历史及其争议 知道如何去检测新的疾病吗？需要多久才能清楚知道与正在处理的新疾病有关的情况？这些问题并不容易回答。1997年流行的猪瘟在许多兽医看来是一个“新”的疾病;缺乏实际经验意味着,当其以一个新的面容出现时,尽管它可能有着众所周知的了解基础,但还是需要花费大量的时间才能得到确诊。     

猪圆环病毒病特点及综合防控

猪圆环病毒病是由圆环病毒引起的一种能侵害猪免疫系统传染病。该病可导致猪产生多种病症,比如断奶仔猪多系统衰竭综合征（PMWS）、猪皮炎与肾病综合征（PDNS）、猪呼吸道疾病综合征（PRDC）、相关性繁殖障碍、相关性肠炎等。近些年来,猪圆环病毒病在我国大范围流行,给广大养殖场带来重大经济损失。本文从病原学、流行病学、临床症状、病理变化以及综合防控多个方面对该病进行了综述,以期能够为养殖从业者防治该病提供参考。     

猪圆环病毒病与防控

近年猪圆环病毒病的发病几率呈逐渐上升趋势,不仅降低养猪行业的经济效益,还对养猪行业的可持续发展造成一定的影响.该病的病原是圆环病毒Ⅱ型病毒,多因猪体质下降感染病毒致病.本文对猪圆环病毒病进行了分析,并提出了有效的防控措施.     

亚洲猪圆环病毒病的控制

亚洲是一个异彩纷呈及充满活力的大陆,其人口数量以及生猪数量都在大量的增加.十大顶尖的生产商中有五个是在亚洲,同样,十大进口商中也有一半是在这里,亚洲还是生猪和猪肉的主要来源地. 亚洲的养猪模式与猪圆环病毒病(PCVD)的呈现有很大的关系.比如,不同年龄阶段的猪混养在单一的场所、对猪圆环病毒2型(PCV2)感染引起的多种疾病均采用同一种理想药方,以及多种引发PCVD的因素.     

一种新猪病:猪圆环病毒感染

猪圆环病毒感染是最近新发现的一种传染病 ,该病对猪的感染率较高 ,在世界范围内广泛发生 ,引起了人们的高度重视。本文就近几年来国内外对其病原学、流行病学、临床症状和病理变化以及诊断、预防等研究情况作一综述     

猪圆环病毒病的防控

猪圆环病毒(PCV)属于圆环病毒科圆环病毒属,为环状、单股DNA病毒,含1767-1768个核苷酸,是动物病毒中最小的一员.圆环病毒分1型和2型,其中2型(PCV2)致病性明显大于1型,临床表现的相关性疫病主要由2型引起.     

Porcine circovirus diseases

Porcine circovirus type 2 (PCV2) is a member of the family Circoviridae, a recently established virus family composed of small, non-enveloped viruses, with a circular, single-stranded DNA genome. PCV2, which is found all over the world in the domestic pig and probably the wild boar, has been recently associated with a number of disease syndromes, which have been collectively named porcine circovirus diseases (PCVD). Postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis and nephropathy syndrome (PDNS) and reproductive disorders are the most relevant ones. Among them, only PMWS is considered to have a severe impact on domestic swine production. PMWS mainly affects nursery and/or fattening pigs; wasting is considered the most representative clinical sign in this disease. Diagnosis of this disease is confirmed by histopathological examination of lymphoid tissues and detection of a moderate to high amount of PCV2 in damaged tissues. Since PMWS is considered a multifactorial disease in which other factors in addition to PCV2 are needed in most cases to trigger the clinical disease, effective control measures have focused on the understanding of the co-factors involved in individual farms and the control or elimination of these triggers. PDNS, an immuno-complex disease characterized by fibrino-necrotizing glomerulonephritis and systemic necrotizing vasculitis, has been linked to PCV2, but a definitive proof of this association is still lacking. PCV2-associated reproductive disease seems to occur very sporadically under field conditions, but it has been characterized by late-term abortions and stillbirths, extensive fibrosing and/or necrotizing myocarditis in fetuses and the presence of moderate to high amounts of PCV2 in these lesions. Taking into account that scientific information on PCV2 and its associated diseases has been markedly expanded in the last 8 years, the objective of this review is to summarize the current state of knowledge of the most relevant aspects of PCV2 biology and PCVD.     

Porcine circovirus 2-associated disease in Eurasian wild boar.

Porcine circovirus 2 (PCV2) was first identified in high-health herds of domestic swine and was associated with a debilitating disease called postweaning multisystemic wasting syndrome (PMWS). Most subsequent studies have indicated that PCV2 infects only swine but there is little information on porcids other than improved breeds of domestic swine. Multisystemic disease was reported in a group of Eurasian wild boars raised under free-range conditions. Affected young pigs had pneumonia and enteritis and were cachectic. Porcine circovirus 2 was identified in affected tissue by immunohistochemistry and in situ hybridization, and a PCV2-like virus was isolated from pooled organs. The open reading frame (ORF2) of the isolated PCV2 had a 98.7% homology with the ORF2 of a reference PCV2 isolate. These diagnostic data indicate that PCV2 can infect and cause disease in Sus scrofa subspecies other than domestic swine.     

Porcine circovirus induces B lymphocyte depletion in pigs with wasting disease syndrome

To disclose the mechanism of cellular injury following porcine circovirus (PCV) infection, 12 pigs were examined by the terminal deoxynucleotidyl transferase-mediated dUTP-nick end labeling (TUNEL) method and immunohistochemistry. Histologically, the lymphoid tissues were characterized by marked apoptosis of lymphocytes, lymphocyte depletion, and macrophages and giant cells containing numerous inclusion bodies with or without apoptotic bodies. Immunohistochemically, there were many lysozyme-positive macrophages in the lymphoid follicles, while the number of CD79a-positive B lymphocytes was scanty. Apoptotic cells, which were proved to be TUNEL positive, revealed CD79a positivity. Although detectable mainly in the cytoplasm of macrophages, PCV antigens were found also in the nuclei of macrophages and apoptotic lymphocytes. Ultrastructurally, the presence of PCV virions was confirmed in apoptotic bodies phagocytosed by macrophages. These findings suggested that lymphocyte depletion with apoptotic death of B lymphocytes was caused by PCV, and that some of the inclusion bodies were phagolysosomes derived from the apoptosis. Thus, PCV may trigger the development of wasting disease syndrome by producing an immunocompromised state in pigs.     

猪圆环病毒检测技术研究进展

猪圆环病毒(PCV)分为1型(PCV-1)和2型(PCV-2).PCV-1一般认为无致病性,而PCV-2是断奶仔猪多系统衰竭综合征(PWMS)的主要致病因子;此外,猪皮炎肾病综合征(PDNS)、仔猪先天性震颤(CT)等疾病也与PCV-2有关.在临床上PCV-2常与猪繁殖与呼吸综合征病毒(PRRSV)、猪细小病毒(PPV)、猪流感病毒(SIV)等病毒混合感染,PCV-2相关疾病已经给世界养猪业造成了巨大的经济损失.然而,由于该病常常和上述几种疾病混合感染,且其临床症状十分类似,所以仅仅依靠单一的临床症状或者某一种实验室诊断方法并不能有效的检测该病.因此,将目前诊断PCV-2的各种方法集中起来,分析其优缺点,从而可以根据实验室的具体情况采取合适的方法是诊断该病的关键.论文就目前PCV-2的检测技术做一介绍.     

猪圆环病毒检测技术研究进展

猪圆环病毒病是由猪圆环病毒(PCV)感染引起的,以免疫抑制为特征的一类病毒性传染病,临床表现主要是由PCV-2引起的仔猪断奶后多系统衰竭综合征。文章就近几年国内外对该病毒检测技术,包括病毒分离、电镜观察、聚合酶链式反应、间接免疫荧光试验、免疫组织化学技术、酶联免疫吸附试验、原位核酸杂交试验等的研究进展做了阐述。     

猪圆环病毒感染后部分免疫器官的组织化学检测

采用非特异性酯酶和甲基绿-派洛宁两种染色方法,对40头猪的淋巴结、脾脏和扁桃体进行研究,利用细胞计数软件,统计分析T、B淋巴细胞的数量变化。结果表明,圆环病毒可导致机体淋巴组织T细胞和B细胞减少,且淋巴细胞数量的变化受伴发病原的影响,其中猪瘟病毒影响最大,伪狂犬病病毒和猪繁殖与呼吸综合征病毒次之。     

猪圆环病毒2型研究进展

猪圆环病毒2型（PCV2）已成为家猪和野猪常见的一种病毒病,由其引起的疾病称为猪圆环病毒相关疾病（PCVAD）,自猪圆环病毒相关疾病（PCVAD）暴发以来,已成为近年来阻碍养猪业发展的重要传染病。为了进一步了解该病,以便更好地预防和控制该病,本文从病原学、流行病学、致病机理、临床症状、防控措施等方面进行了综述。