Wheat quality traits and quality parameters of cooked dry white Chinese noodles

Dry white Chinese noodle (DWCN) is widely consumed in China, and genetic improvement of DWCN quality has become a major objective for Chinese wheat breeding programs. One hundred and four bread wheat cultivars and advanced lines, including 88from major Chinese wheat-producing areas, were sown in two locations for two years. Their DWCN quality, as evaluated by trained panelists, was studied to determine the relationship between wheat quality parameters and DWCN quality attributes. In general, the cultivars and advanced lines used in this study are characterized with acceptable protein content, but accompanied with weak-medium gluten strength and poor extensibility, and substantial variation is observed for all grain and DWCN quality characters. On average, Australia and USA wheat performed better DWCN quality than Chinese wheats. Simple correlation analysis indicated that both grain hardness and Farinograph water absorption were negatively associated with cooked DWCN color, appearance, smoothness, and taste. Flour whiteness and RVA peak viscosity was positively associated with all DWCN parameters, and their correlation coefficients (r) with DWCN score are 0.34 and 0.41, respectively. Their positive contributions to DWCN quality were mostly through improved color, appearance, smoothness, and taste. Farinograph mixing tolerance index (MTI) and softening were negatively associated with all DWCN quality parameters, and their correlation coefficients with DWCN score are –0.50 and–0.54, respectively. Further analysis indicated that association between protein content, Zeleny sedimentation value, Farinograph stability, and Extensograph extensibility, and DWCN score fit quadratic regression model significantly, with R² 0.12, 0.32, 0.22, and 0.20, respectively. The associations between Zeleny sedimentation value and DWCN's appearance and taste also fit quadratic regression model significantly. This suggests that to certain extent, increased protein content and gluten quality contribute positively to DWCN quality, mostly by improving palatability, elasticity, and stickiness. High flour whiteness, medium protein content, medium to strong gluten strength and good extensibility, and high starch peak viscosity are desirable for DWCN quality. Genetic improvement for flour whiteness, protein quality and starch paste viscosity would increase the DWCN quality of Chinese bread wheat cultivars. This revised version was published online in July 2006 with corrections to the Cover Date.     

小麦面粉、面条色泽与蛋白质组分的关系

明确面条白度与小麦蛋白质组分含量的关系有助于面条品质的改良。以21个小麦品种制成的面粉及面条为材料，分析了面粉及面条色泽与蛋白质组分含量之间的关系，讨论了蛋白质组分中单体蛋白含量、可溶性谷蛋白含量和不溶性谷蛋白含量对小麦面粉及不同工艺阶段面条色泽的影响。研究结果表明，面粉的颗粒度与面粉及面条的L值呈极     

SDS不溶性谷蛋白大聚体含量与和面仪参数的关系

选用品质差异较大的108份主栽品种和高代品系, 测定其谷蛋白聚合体总量(双缩脲法)和贮藏蛋白组分含量及比例(凝胶色谱法, SE-HPLC), 并分析了它们与蛋白质含量、沉降值以及和面仪参数等早代选择参数的相关性。结果表明, 参试材料品质特性和贮藏蛋白组分含量的变异范围都较大。和面仪峰值时间变异范围为1.12~7.19 min, 变幅达6.07 min; 醇溶蛋白总量和SDS可溶性谷蛋白聚合体(EPP)变幅较小, 而SDS不溶性谷蛋白大聚体(UPP)的变幅最大。硬度、蛋白质含量和沉降值与和面峰值时间的相关系数较低(r=0.24~0.49)。醇溶蛋白总量与和面仪参数相关不显著, 而与谷蛋白总量的比值(Gli/Glu)与和面仪参数呈显著负相关(r= –0.52~ –0.61, P＜0.001), 且不受蛋白质含量的影响。SDS不溶性谷蛋白大聚体百分含量(%UPP)与和面仪参数的相关性最高, 相关系数为0.70~0.85(P＜0.001)。SE-HPLC法样品用量少, 自动化程度高, 且%UPP和Gli/Glu为相对值, 与和面仪参数呈高度相关, 可作为面筋强度早代选择的有效指标。     

Variation and association of morphological and biochemical characters in grass pea (Lathyrus sativus L.)

Fifty grass pea land race populations of Ethiopia that were selected based on administrative regions and different altitude classes were used in this study to determine variability in morphological characters, Oxalyl Diamino Propanoic acid(ODAP) – a causative agent of lathyrism, and protein contents and their associations. Highly significant differences were observed among the populations for most of the morphological characters. Populations collected from Gondar region and the higher altitude group (>2550 m) showed the highest mean and coefficient of variation indicating the presence of high genetic diversity in this region and altitude group. ODAP analysis from single plants showed significant variation both within a population and between populations. Four individual plants with low ODAP contents ranging from 0.149% to 0.182% (range within safe level) were identified in populations collected from different regions, indicating the diversity and variation of this trait with in one population and between regions. These low ODAP lines are considered important for further breeding. The association of ODAP with grain yield, biomass, plant height and seed size was negative, suggesting that the selection of tall and late maturing varieties with large seed size and high grain yield potential will enable the development of varieties with low ODAP content. This revised version was published online in August 2006 with corrections to the Cover Date.     

Quality of Ethiopian Durum Wheat Lines in Two Diverse Environments

In Ethiopia, durum wheat is largely used for production of local fermented and flat bread. Two diverse environments (Motta and Adet) were used to evaluate 15 durum wheat genotypes for grain yield and quality traits. The mean flour protein content of genotypes ranged from 10.1 % to 12.5 % and 6.7 % to 8.1 % at Motta and Adet respectively. The mean mixograph development time was 4 min at Motta and 2.8 min at Adet and SDS (Sodium dodecyl sulphate) sedimentation ranged between 10.7 and 32.3 ml across locations. Flour protein content was correlated negatively with mixograph development time and positively with vitreous kernels and single‐kernel hardness at both environments. Mixograph development time was selected to predict the gluten strength. Flour protein content, SDS sedimentation and seed weight were included in a stepwise regression. A prediction model was compiled that explained 69 % of the variation for mixograph development time.     

水分胁迫对不同筋型小麦籽粒品质性状的影响

为了了解不同水分胁迫对不同品质类型小麦主要品质性状的影响,试验选用3个有代表性的强、中、弱筋春小麦品种,在防渗池中进行种植。研究结果表明：小麦籽粒主要品质性状在不同筋型间差异达到极显著水平,在不同水分条件下也存在显著差异。在水分胁迫下,湿面筋含量、粗蛋白含量以及沉淀值均呈显著或极显著下降趋势,而膨胀势显著增大。在试验条件下品质性状间的相关分析结果表明,不同品种间粗蛋白含量与湿面筋含量、沉淀值呈极显著正相关。因此在小麦生态育种和调优栽培研究中,需要重视水分因子对小麦籽粒品质性状影响的不同步性。     

基因型、地点及其互作对内蒙古小麦主要品质性状的影响

选用来自我国春播麦区高、中、低3种筋力类型的9个品种, 于2003和2004年分别种植在内蒙古6个代表性地点, 研究了不同品种在年份和地点间籽粒硬度、蛋白质含量、和面仪参数和淀粉糊化特性等主要品质性状的变化规律。结果表明, 所测品质性状受基因型和地点效应的影响均达极显著水平, 除籽粒蛋白质含量外, 其他品质性状受基因型和地点互作效应的影响达显著或极显著水平。强筋类品种的蛋白质含量、灰分含量、沉降值、和面时间、耐揉性和峰值黏度均较高, 出粉率和稀澥值中等。中筋类品种出粉率、和面时间和耐揉性较高, 灰分含量、峰值黏度和稀澥值较低。弱筋类品种的灰分含量、峰值黏度和稀澥值较高, 籽粒硬度、蛋白质含量、出粉率、沉降值、和面时间、耐揉性低。所有品种品质性状在地点间存在较大差异, 乌海市灰分含量、和面时间和耐揉性高, 籽粒硬度、沉降值、峰值黏度和稀澥值较低。杭锦后旗出粉率高, 蛋白含量和沉降值较低, 其他性状表现中等。呼和浩特市籽粒硬度、蛋白含量、面粉灰分、沉降值、和面时间和耐揉性高, 出粉率、峰值黏度和稀澥值低。赤峰市多数性状表现中等。通辽市籽粒硬度、蛋白质含量、峰值黏度、稀澥值和耐揉性较高, 其他性状表现中等。额尔古纳市蛋白含量和沉降值较高, 和面时间和耐揉性低。初步认为强筋和中筋类品种较适于种植在呼和浩特市与乌海市, 不适于种植在额尔古纳市; 2个弱筋类品种在6个地点均不太适宜种植。     

小麦多酚氧化酶研究进展

小麦面粉和面食品的色度是小麦磨粉品质和面食品的主要评价指标.。特别是面条、馒头、饺子、烙饼等中式食品对白度的要求相当高。.多酚氧化酶(PPO)与小麦面粉及其面食品在加工、贮藏中的褐变直接相关.。同时,多酚氧化酶种类的遗传变异和表达差异也决定小麦籽粒中的PPO总活性高低,从而直接或间接影响面粉的白度。笔者综述了近年来小麦多酚氧化酶生化机理、分布和分子生物学遗传分析以及与品质关系等方面的研究进展,并且根据多酚氧化酶的表达特性,讨论了小麦高白度新品种选育的策略。     

小麦高分子量麦谷蛋白亚基遗传变异及其与品质和其它农艺性状关系的研究

本研究应用SDS-PAGE技术分析了国内外757份供试材料的高分子量（HMW）麦谷蛋白亚基组成。共发现65种HMW亚基组成，其中53种为常见型，12种为罕见型。国外品种的亚基组合数多于国内品种，国内育成品种又多于国内地方品种。作者还发现了部分罕见亚基，即由Glu-D1编码的亚基2.2+12，2+10，2，12以及前人未曾发现的3个亚基。研究     

Suitability of Chinese wheat cultivars for production of northern style Chinese steamed bread

Fifty-six bread wheat cultivars and advanced lines from major Chinese wheat growing regions and 10 Australian cultivars were grown in Anyang located in Yellow and Huai Valleys and Chengdu located in Yangtze region. The genotypes were examined for their suitability to produce northern style Chinese steamed bread (CSB) and used to investigate the association between wheat quality traits and performance of northern style CSB under manual and mechanized processing conditions. Anyang-sown wheat samples showed better grain quality characteristics and CSB quality than the Chengdu-sown materials. These differences were largely due to adverse climatic conditions prevailing in Chengdu that resulted in the deterioration of flour whiteness, Farinograph stability, and starch quality. Therefore, Chengdu was generally unfavorable for producing good quality wheat. However, significant variability among cultivars was observed in Chengdu, and Batavia, Dollar bird, and Tasman from Australia and Jing 411, Xiaoyan6, and Shaan 229 from China showed very good CSB quality under manual conditions, and Hartog, Batavia, Tasman, and Vulcan from Australia, and Jing 411 and Dongfeng 1from China were identified to confer good CSB quality under mechanized conditions. The wide range of CSB quality variations indicates that genetic improvement of CSB quality is possible in both environments. Protein content, gluten strength, and extensibility were positively associated with loaf volume and steamed bread elasticity. The relationship between gluten strength, extensibility, and appearance and stickiness were highly dependent on processing methods, i.e., negatively using a manual method and positively or slightly negatively using a mechanized process. Therefore, wheat quality requirement for CSB depends on CSB processing conditions. Medium protein content and medium-to-strong gluten strength with good extensibility is desirable for mechanized methods, but weak-to-medium gluten type for manual methods. High flour whiteness and RVA peak viscosity was found desirable for CSB quality regardless of the processing method used. Flour whiteness, falling number, and peak viscosity appeared to be more crucial in determining CSB quality in Chengdu, although protein content and Farinograph stability also contributed to appearance, elasticity, and stickiness. This revised version was published online in July 2006 with corrections to the Cover Date.     

Variation in quality traits in common wheat as related to Chinese fresh white noodle quality

The variation in grain compositional traits related to Chinese fresh white noodle (CFWN) quality was examined in 25 leading Chinese common wheat cultivars and advanced lines. Large variations were observed in flour yield, flour particle size, protein and ash contents, farinograph and rapid visco-analyzer (RVA) parameters, flour colour components, and polyphenol oxydase (PPO) activity. An improved sensory method was proposed for adequately evaluating CFWN quality of common wheat flour based on methodology used in the Japanese quality scoring system for white salted noodles, but with major modifications in the score weight given to each noodle quality parameter. Large differences in CFWN quality were observed among Chinese wheat cultivars. The correlation coefficients (r) between starch paste breakdown (RVA) and noodle viscoelasticity and smoothness were 0.63 and 0.59, respectively, suggesting that in breeding wheat for high CFWN quality, the starch pasting properties are of major importance. Low ash content was significantly correlated (r = – 0.66) with bright white noodle colour.     

Changes in wheat seed storage protein fingerprint due to soil mineral content

Wheat seed storage protein fingerprint is used to determine the gluten protein pattern in studies aimed at improving flour quality. Wild wheat with high seed protein content is used extensively in wheat breeding programs. Although the wild wheat growth and protein content may be influenced by environmental conditions, the gluten-protein pattern is generally considered as indicative of a genotype, without the superimposition of environmental influences. The effects of soil type, habitat, and deficiencies of N, P, K and S on seed storage protein composition were examined in nine accessions of wild wheat (Triticum turgidum var. dicoccoides) and three varieties (two T. aestivum and one T. durum). Soil from ten natural habitats of the wild wheat that had not previously received any fertilizers or manures was sampled and used to grow wheat in a greenhouse. Seed storage protein composition was characterized by SDS-PAGE. Although deficiencies in soil nutrient caused variations in the seed storage proteins, the genotype was the main factor determining the seed storage protein composition. Seed storage protein composition of genotypes varied when grown under different mineral nutrient conditions. Only one genotype was stable showing almost identical protein patterns under all growing conditions studied without any qualitative change in fingerprint pattern. In the other genotypes, as well as the cultivars, the seed storage protein was affected at least to some extent by the soil. The ‘soil effect’ is summarized in terms of three main quantitative changes in the seeds: 1 – the relative amounts of the high-molecular-weight proteins; 2 – the relative amounts of proteins in the range of 45 and 65 kD; 3 – the percentage distribution of the HMW glutenin and other groups of seed storage proteins. The soild induced also qualitative differences in the composition of seed storage proteins, mostly in those of 45–65 kD. These differences were observed whenever a deficiency of S, N, P, K or Mg was identified. Therefore, in breeding programs that use seed storage protein fingerprints of wild wheat germplasms should be exercise caution when the germplasms selected from wild habitats. This revised version was published online in July 2006 with corrections to the Cover Date.     

黄淮麦区部分推广小麦品种面条加工品质性状的研究

为了探讨黄淮麦区推广小麦品种面条加工品质性状的表现水平、遗传多样性状况并评选优质面条品种,本研究对黄淮麦区42个小麦主栽品种的26项籽粒或面粉品质指标和面条加工品质性状进行了分析,结果表明,供试品种面条总分平均75.3分,最高分为89分,各个单项指标变异幅度均很大,遗传多样性比较丰富,通过遗传改良我国黄淮麦区小麦面条品质有一定的潜力.适合做优质面条的品种有花培5号、周18、周13和邯郸6172.     

强筋小麦磨粉各出粉点的面粉品质特性研究

采用布勒实验磨制粉,测定6个强筋小麦品种不同出粉点面粉的品质特性。结果表明,不同出粉点面粉的品质特性具有明显差异及变化规律。三道心磨粉的灰分含量、蛋白质含量、面糊色泽a*值和b*值、破损淀粉含量、面团形成时间和吸水率随出粉点后移均呈升高或增加趋势;而湿面筋含量、面糊色泽L*值、面团稳定时间、延伸性和最大抗延阻力随出粉点后移而降低。三道皮磨粉的蛋白质含量、湿面筋含量、破损淀粉含量、面团吸水率、稳定时间、拉伸面积、延伸性随出粉点后移呈上升趋势;而面团拉伸参数和面包烘焙品质均高于心磨粉和统粉。不同出粉点的蛋白质与湿面筋含量呈极显著正相关(r=0.81**);灰分含量与面糊色泽的L*值呈极显著负相关(r=－0.89**);破损淀粉含量与吸水率呈极显著正相关(r=0.86**)。研究表明,通过对各出粉点取舍及混配,能够克服我国强筋小麦某些指标缺陷,以满足面包专用粉各项指标要求。     

CIMMYT 145份小麦种质资源的鉴定及杂交利用

为了拓宽和丰富小麦种质资源遗传多样性,提高小麦产量和品质,在大田对国外引进的145份小麦种质资源进行了农艺性状和品质性状的鉴定和评价。结果表明,这些材料基本属弱春性类型。在山西南部麦区,可推迟播期于10月下旬种植,作为育种材料利用。主要农艺性状的单株穗数、穗粒数、千粒重和产量变异系数分别为15.7%、16.03%、12.23%和23.96%,多数材料籽粒综合品质较好,尤其面筋品质好,筋力强。这些材料的农艺性状和品质性状具有丰富的遗传多样性。株高和千粒重与产量的相关系数最大,分别为0.5032和0.4178,达极显著水平,是提高产量的关键因素。综合3年试验结果,筛选出产量超过3750 kg/hm2有32份,鉴选出8个组合的相应强筋力品系和35个综合性状表现较优异品系,其突出优异性状表现植株穗大,小穗结实好,穗粒多,可作为小麦育种的重点种质材料利用。通过播期调节花期,利用CIMMYT材料合理杂交配组,已选育至F3代、BC2F1代和BC1F1代,有望育成优质大穗丰产抗旱创新材料及新品系。     

Seed quality attributes of food-grade soybeans from the U.S. and Asia

Diversity of food-grade soybeans is critical for utilization of genetic resources in cultivar development, germplasm enhancement, and end-product commercialization. The objective of this study was to assess seed quality attributes and phenotypic variability among 54 U.S. and 51 Asian food-grade cultivars and breeding lines. The results showed greater genetic diversity of protein content, calcium content, seed hardness, and seed size uniformity than other quality traits in both small- and large-seeded genotypes evaluated in this study. Among the small-seeded soybeans, the U.S. genotypes were more diverse and exhibited higher swell ratio and oil content but lower stone seed ratio and protein content than Asian accessions. Among the large-seeded accessions, U.S. genotypes had higher stone seed ratio and oil content but lower swell ratio and protein content, and were less diverse than Asian genotypes. The characterization of diverse food-grade soybeans will facilitate parent selection in specialty soybean breeding.     

小麦品质性状与面包和面条品质关系分析

改良面包和面条品质是北方冬麦区的主要育种目标,了解二者的品质异同有助于提高育种效率。本研究以81份冬小麦品种品系为材料,比较了小麦品质性状与面包和面条品质表现的关系。结果表明,籽粒硬度、蛋白质含量、SDS沉降值、形成时间、稳定时间、拉伸面积和抗延阻力是影响面包和面条品质的共同性状,但它们对面包、面条品质     

An agronomic and seed-quality evaluation of noug (Guizotia abyssinica Cass.) germplasm in Ethiopia

Noug (Guizotia abyssinica Cass.), a member of the Compositae family, is grown as an oilseed crop in Ethiopia. The crop grows best on poorly drained, heavy clay soils. This study investigates the genetic variability of agronomic and seed quality characteristics of 241 noug germplasm collections from different parts of Ethiopia evaluated in a field test at Ghinchi, near Addis Ababa, Ethiopia, in 1992. The maturity of the noug accessions ranged from 132 to 168 days. Seed oil contents ranged from 39.8 to 46.9% with linoleic acid being the major fatty acid of the oil (76.6% of total fatty acids). The results of this field study indicated that genetic differences for maturity existed among the noug accessions. Oil content variation was continuous, without clear separation of accessions into oil content groups. It is concluded that the genetic variation observed among these accessions could be utilized in a breeding programme to develop high-yielding, well-adapted, high-oil-content noug cultivars for production in Ethiopia.     

Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars

Genetic diversity is the basis for successful crop improvement and can be estimated by different methods. The objectives of this study were to estimate the genetic diversity of 30 ancestral to modern hard red winter wheat (Triticum aestivum L.) cultivars adapted to the Northern Great Plains using pedigree information, morphological traits (agronomic measurements from six environments), end-use quality traits (micro-quality assays on 50 g grain or milled flour samples for the six environments), and molecular markers (seed storage proteins separated using SDS-PAGE, 51 SSRs, and 23 SRAP DNA markers), and to determine the relationships of genetic distance estimates obtained from these methods. Relationships among diversity estimates were determined using simple (Pearson) and rank (Spearman) correlation coefficients between distance estimates and by clustering cultivars using genetic-distances for different traits. All methods found a wide range in genetic diversity. The genetic distance estimates based on pedigree had the highest values due to possible over-estimation arising from model assumptions. The genetic diversity estimates based on seed storage protein were lowest because they were the major determinants of end-use quality, which is a highly selected trait. In general, the diversity estimates from each of the methods were positively correlated at a low level with the exceptions of SRAP diversity estimates being independent of morphologic traits (simple correlation), SDS-PAGE, and SSR diversity estimates (rank correlation). However, SSR markers, thought to be among the most efficient markers for estimating genetic diversity, were most highly correlated with seed storage proteins. The procedures used to accurately estimate genetic diversity will depend largely upon the tools available to the researcher and their application to the breeding scheme.     

小麦诱发突变品质育种的探讨

小麦品质育种不仅要注意提高蛋白质含量,更应大力改进小麦面筋的质量.利用诱发突变手段可以获得营养品质和加工品质优良的新品种,如晋麦23号、晋麦22号和原A14等.能否充分发挥育成品种的优质性状,取决于相应的生态条件和栽培水平是否适宜.诱发突变品质育种成败的关键在于试材与亲本的选择.亲本中要具有高蛋白遗传基础和稳定的品质性状,并要克服亲缘单一化和不利基因的干拢.产量与品质的矛盾,可以通过诱发突变与杂交相结合的方式,加以缩小或取得一定程度的协调.