猪顺气穴插枝对T淋巴细胞的影响

猪顺气穴插枝疗法对猪的感冒发热、胃肠炎、眼结膜炎等的疗效一般可达90％左右,但对其治病机理,目前研究尚少。我们用免疫学方法观察了插枝疗法对猪T淋巴细胞的影响。材料与方法 (一)试验动物 2～3月龄的临床健康幼猪45头,第一批20头,第二批25头;健康公绵羊1只。     

插枝牛顺气穴对唾液和血清淀粉酶活性的影响

用插枝法插枝牛顺气穴后，测定了唾液和血清淀粉酶活性的变化。结果：唾液淀粉酶活性显著升高（P＜0.01），且在插枝后30min达到最高值；血清淀粉酶活性在插枝后15－45min时也升高。这对进一步研究针刺顺气穴及相关穴位对动物消化系统的影响提供了依据。     

电针穴位和肌肉对绵羊血液指标的影响比较

为了探讨针灸疗法的作用机理和穴位的相对特异性 ,本试验以健康绵羊为试验动物 ,通过电针穴位组和肌肉组并在针后不同时间采血 ,比较不同部位的电针对绵羊血液生理指标的影响。现将结果报告如下。1　材料与方法1 .1　试验器材　 73- 1 0型兽用电疗针麻机 (广东海南电子医疗仪器厂制造 ) ;72 1型分光光度计 (上海第三分析仪器厂 ) ;6～ 1 0 cm兽用针灸针等。1 .2　试验试剂　均由生理实验室提供。1 .3　试验动物及分组　本院动物室饲养的藏羊(1～ 3岁 ) 1 0只 ,每组 5只 ,试验期间动物的饲料、饮水、运动条件相对稳定。1 .4　穴位和肌肉的选…     

顺气穴插枝治疗渤海黑牛瘤胃臌胀

顺气穴插枝疗法是根据祖国传统医学概念,利用枝条在穴位的刺激作用,达到通络、调气血的目的,使患畜解除疾病,恢复健康.笔者多年来利用顺气穴插枝治疗渤海黑牛瘤胃臌胀126例,疗效显著,介绍如下:     

顺气穴插枝治猪病案例

<正>顺气穴插枝疗法治病猪,经临床和实验研究证明,既能调节机体免疫不足,使免疫低下的动物其免疫力增加,又能抑制过度的免疫反应。增强机体免疫功能的机理主要表现在调节特异免疫应答和非特异免疫两个方面。插枝疗法对特异免疫的调节。体现在插枝后能调节免疫蛋白的分泌合成,促进淋巴细胞的活化、增殖并发挥功能。顺气穴是猪传统针灸疗法的穴位,经笔者多年临床验证,可治猪呼吸系统(如急性支气管炎、咽炎、鼻炎等)和消化系统(如消化不良、食欲不     

顺气穴插枝治疗牛胃肠病和眼病的实验报告

顺气穴插枝疗法是流传在民间的一种治疗牛病的针灸治疗方法。1957年农业部畜牧兽医总局编写的《兽医针灸汇编》,以及以后出版的《中兽医针灸学》、《兽医针灸手册》等书均有收载。据云:顺气穴有治疗感冒、牛臌胀、角膜炎等病,特别对牛臌胀有顺气作用,故名顺气穴。1974年以来,我们曾用顺气穴插枝治疗牛宿草不转(瘤胃食滞)7例,气胀(瘤胃臌胀)4例,     

顺气穴插枝术的解剖所见

继“顺气穴插枝治疗牛眼病400例的体会”一文在《中兽医医药杂志》1991年第1期刊登后,笔者又对顺气穴插枝术进行了新的探索,即作了解剖观察,现将情况作以介绍。解剖的方法是,选一屠宰的黄牛,宰后按常规方法在顺气穴插入枝条,然后顺枝条走向进行解剖,发现当枝条插入顺气穴约5cm 深,就在同侧鼻孔内露出,用手指在该鼻孔鼻纵沟内就能摸到枝条,两侧均一样。     

顺气穴插枝疗法治疗猪病

笔者自1987年以来,采用顺气穴插枝疗法治愈猪病53列,其中猪便秘28例,消化不良5例,肺炎2例,胃炎8例,感冒10例,获得了满意的效果。1　穴位　口腔上硬腭(硬腭正中线有一纵行的腭缝,腭缝两侧有许多横行的腭褶),在腭缝的前端与切齿之间有一突起称切齿乳头,在切齿乳头两侧为鼻腭管开口处,二孔与鼻腔相通,此即顺气穴。2　插枝材料　采用生的柳枝、桃枝或樟树枝条,其它各种纤细的树枝条也可。枝条长短、粗细、视猪的大小而定,一般长约10～15cm,粗约0.15～0.2cm。选用软硬适中而且较挺直的枝条,剥去叶痕和外皮,使其光滑,头部削成钝形备用。3　操作　…     

顺气穴插枝对牛羊生理功能作用的实验初报

作者近年来,在兽医临床工作中,应用顺气穴插枝法,治疗牛胃肠病37例,取得了满意的效果。为了进一步探索其对畜体功能的影响及治病原理,进行了健康牛、羊22例的实验,通过自身插枝前后的体温、呼吸、心跳、瘤胃蠕动、瘤胃液、血液等12项指标的对比观察结果,插枝后有增强瘤胃蠕动和白血球总数增加的作用。     

生长抑素基因工程苗对绵羊外周血液某些代谢物浓度和激素水平的影响

选择4～5月龄体重相近的杂种雄性去势绵羊16只,随机分成试验组和对照组,对照组平均体重为21.25kg,试验组平均体重为21.54kg。试验组绵羊一次性皮内多点注射生长抑素基因苗(SS苗)1mL,对照组绵羊皮内多点注射生理盐水1mL。试验共持续63d。试验结束时对照组平均体重27.79kg,试验组平均体重29.71kg。注射SS苗和生理盐水当天(0d)、28d、51d、63d,早晨放牧前颈静脉采血制备血浆,分析代谢物及激素含量。结果显示,SS苗免疫对动物葡萄糖和非酯化脂肪酸(NEFA)和尿素氮浓度无明显影响;SS苗免疫后28d时,IGF-I、GH浓度分别比对照高10.81%和5.23%,在51d时高57.36%(P<0.01)和35.73%(P<0.05)。     

Comparison of in vitro and in situ methods in evaluation of forage digestibility in ruminants

The objective of this study was to compare the application of different in vitro and in situ methods in empirical and mechanistic predictions of in vivo OM digestibility (OMD) and their associations to near-infrared reflectance spectroscopy spectra for a variety of forages. Apparent in vivo OMD of silages made from alfalfa (n = 2), corn (n = 9), corn stover (n = 2), grass (n = 11), whole crops of wheat and barley (n = 8) and red clover (n = 7), and fresh alfalfa (n = 1), grass hays (n = 5), and wheat straws (n = 5) had previously been determined in sheep. Concentrations of indigestible NDF (iNDF) in all forage samples were determined by a 288-h ruminal in situ incubation. Gas production of isolated forage NDF was measured by in vitro incubations for 72 h. In vitro pepsin-cellulase OM solubility (OMS) of the forages was determined by a 2-step gravimetric digestion method. Samples were also subjected to a 2-step determination of in vitro OMD based on buffered rumen fluid and pepsin. Further, rumen fluid digestible OM was determined from a single 96-h incubation at 38°C. Digestibility of OM from the in situ and the in vitro incubations was calculated according to published empirical equations, which were either forage specific or general (1 equation for all forages) within method. Indigestible NDF was also used in a mechanistic model to predict OMD. Predictions of OMD were evaluated by residual analysis using the GLM procedure in SAS. In vitro OMS in a general prediction equation of OMD did not display a significant forage-type effect on the residuals (observed - predicted OMD; P = 0.10). Predictions of OMD within forage types were consistent between iNDF and the 2-step in vitro method based on rumen fluid. Root mean square error of OMD was least (0.032) when the prediction was based on a general forage equation of OMS. However, regenerating a simple regression for iNDF by omitting alfalfa and wheat straw reduced the root mean square error of OMD to 0.025. Indigestible NDF in a general forage equation predicted OMD without any bias (P ≥ 0.16), and root mean square error of prediction was smallest among all methods when alfalfa and wheat straw samples were excluded. Our study suggests that compared with the in vitro laboratory methods, iNDF used in forage-specific equations will improve overall predictions of forage in vivo OMD. The in vitro and in situ methods performed equally well in calibrations of iNDF or OMD by near-infrared reflectance spectroscopy.     

大庆市羊东毕吸虫病流行病学调查

对大庆市羊东毕吸虫病的流行病学进行了调查研究。结果表明，大庆有3种东毕吸虫，即程氏东毕吸虫（O.cheni)、土耳其斯坦东毕吸虫（O.turkestanica)和土耳斯坦东毕吸虫结节变种（O.turkestanican var.thuberculata)；中间宿主螺类有1种，即卵萝卜螺；东毕吸虫病在大庆分布很广，所调查的4区2县均有发生，羊东毕吸虫平均感染率分别为50．33％，平均死亡率为21．12％，大庆羊急性病例发病时间为8月上旬至10月下旬，慢性病例为11月上旬至翌年2月中旬。     

ELISA试验在防制猪伪狂犬病中的应用

猪伪狂犬病是由伪狂病病毒(Pseudorabies virus PRV)引起的急性传染病。其特征是成年猪常为隐性感染，可有流产、死胎、呼吸系统症状，新生仔猪除神经症状外还可侵害消化系统，并伴大批死亡。本病已经给全世界的养猪业造成严重的损失，我国许多地区已报道该病。为进一步探讨该病的控制、净化措施，我们对12个养猪场共856分血清采用美国嗍公司的ELISA试剂盒进行了抗体检测。     

Estimate of economic impact of fowl cholera in turkeys in Georgia in 1986

Information gathered from cases of fowl cholera (FC) in commercial turkey flocks through case records, flock records, and telephone and mail surveys was used to estimate disease costs. The cost to the Georgia commercial turkey industry in 1986 from preventive measures, treatment of outbreaks, and production losses from the disease was estimated at $634,545. The cost of FC per kg of live production was estimated to be $0.015.     

中国圈养野生动物疫苗使用调查

2005年对中国动物园协会单位中的18家动物园饲养野生动物疫苗免疫情况进行调查。受调查动物园在野生动物疫苗使用和动物种类方面具有一定的代表性。调查结果显示动物园动物现共使用18类36种疫苗,预防31种疫病。其中哺乳动物使用14类24种疫苗,预防24种疫病;禽类使用4类12种疫苗,预防7种疫病。使用范围最广的有禽流感、犬瘟热疫苗等、新城疫疫苗、猫瘟热疫苗等。共有24目58科动物接种疫苗,其中食肉动物类1目8科,食草动物类3目9科,杂食动物类2目3科,禽类18目38科。研究结果提示应加强动物园之间疫病信息交流、防疫资源的利用、疫病监测和研究,加大对动物园动物疫病的研究投入,逐步建立动物园动物统一的防疫规程。     

Use of cyclocryotherapy in management of glaucoma in dogs

In 5 cases of glaucoma (2 from trauma, 2 from narrowed drainage angles, 1 secondary to lens extraction), cyclocryotherapy was used to control intraocular pressure. In all cases the intraocular pressure decreased, with the usual result being a cosmetic and painless but blind eye.     

Efficacy of parvaquone in the treatment of naturally occurring theileriosis in cattle in Iraq

Summary Parvaquone was tested in cattle infected withTheileria annulata when they were presented at clinics in the vicinity of Baghdad. Out of over 200 cases presented with suspected theileriosis between July 1984 and July 1985, the drug was used in 45 cases where theileriosis was confirmed by microscopic examination of blood and lymph node biopsy smears. Twenty seven of the cases were considered mild and 18 cases severe. Weights of the cattle were estimated and parvaquone was administered by intramuscular injection at a nominal dose of 20 mg/kg. A single treatment with parvaquone was used in 25 cases and 20 cases were treated twice but there was no correlation between severity of disease and the number of treatments given. Twelve cases (27%) also received antibacterial therapy. All cases were in exotic cattle or cattle born from exotic (imported) cattle and 64% of the cases were in cattle under six months of age. Temperatures dropped immediately after treatment and the majority were normal (below 39.5°C) by two to three days after the first treatment. Of the 45 cases treated 43 recovered. This compares very favourably with a previously reported mortality of 66% in untreated imported cattle in Iraq.

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Eficacia Del Parvaquone En El Tratamiento De La Theilerosis Natural En Ganado En Iraq

Resumen Se trató ganado infectado conTheileria annulata en forma natural con parvaquone, en clínicas veterinarias localizadas en la vecindad de Baghdad. De 200 casos admitidos con sospecha de theileriosis, entre julio 1984 y julio 1985, la droga se usó en 45 casos confirmados mediante el examen microscópico de frotis de sangre y biopsias de ganglios linfáticos. Veintisiete de los casos se consideraron moderados y 18 casos severos. Se estimaron los pesos de los animales enfermos, suministrandoles parvaquone vía intramuscular en dosis nominales de 20 mg/kg (rango 16–35 mg/kg). Se utilizó un solo tratamiento con parvaquone en 25 casos clínicos, mediante la aplicación de una sola inyección y se aplicaron dos inyecciones en 20 casos (doble tratamiento), sin que se encontrara correlación alguna entre la severidad de la enfermedad, recuperación y número de tratamientos. Doce casos (27%) recibieron también algún tratamiento antibacteriano. Todos los casos de theileriosis se presentaron en ganado exótico o nacido de ganado exótico (importado) y 64% de los casos ocurrieron en ganado bajo los seis meses de edad. La temperatura bajó inmediatamente después del tratamiento, normalizándose dos o tres días después de tratamiento (bajo 39.5°C). De los 45 casos tratados 43 se recuperaron. Se informa una mortalidad previa de 60% en ganado importado no tratado contra theileriosis en Iraq.

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Efficacite De La Parvaquone Pour Le Traitement De La Theileriose Naturelle Des Bovins En Irak

Résumé La parvaquone a été essayée pour le traitement de bovins infectés parTheileria annulata lorsqu'ils étaient présentés aux cliniques des environs de Bagdad. Sur plus de 200 cas présentés pour suspicion de theilériose entre juillet 1984 et juillet 1985, le médicament a été utilisé dans 45 cas où la theilériose était confirmée par l'examen microscopique du sang et de biopsies de ganglions lymphatiques, 27 cas étaient considérés comme des atteintes modérées et 18 comme des cas graves. Les poids des animaux étaient estimés à l'oeil et la parvaquone administrée par voie intra-musculaire à la dose normale de 20 mg/kg (soit 16 à 35 mg/kg). Un traitement unique à la parvaquone a été pratiqué dans 25 cas et il a du être renouvelé dans 20 cas mais il n'y avait pas corrélation entre la gravité des symptômes et le nombre de traitements requis. Dans 12 cas (27%) une thérapie antibactérienne a également été mise en oeuvre. Tous les malades étaient des animaux importés ou issus d'animaux importés et dans 64% des cas les animaux étaient âgés de moins de 6 mois. Les températures ont chuté immédiatement après le traitement et, pour la plupart, étaient normales (moins de 39°5) deux à trois jours après le traitement—sur 45 animaux traités, 43 ont guéri. Ces résultats apparaissent très favorables par rapport à la mortalité de 66% rapportée antérieurement et affectant les bovins non traités en Irak.