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1.
A study was undertaken in five draught horses of 648±33 kg body weight to find the effects of continuously pulling loads on their cardiovascular, respiratory and metabolic responses. A cart equipped with an odometer, for measuring distance, and a hydraulic dynamometer, for measuring draught force, was used. Heart and respiration rates and rectal temperatures were recorded. Blood samples for measuring arterial and venous pH and blood gases, haemoglobin, glucose and lactic acid concentrations and the serum activity of the enzymes creatine phosphokinase (CK), lactate dehydrogenase, aspartate aminotransferase and alkaline phosphatase were taken before exercise and immediately after each journey (morning and afternoon) of the daily work.Draught exercise, with loads which generated forces of between 0.57 and 0.59 kN, at speeds of 1.60 to 2.11 m/s, for 8 h daily for five consecutive days, with resting intervals of 10 min each hour, was well tolerated. Exercise tolerance was evaluated from the recovery from the changes observed in the biochemical and physiological parameters induced by the work. The analysis of these showed that, when the horses were subjected to prolonged periods of resting, their loss of fitness for work was shown by significant increases in the serum activity of muscle-derived enzymes and in blood lactate concentrations during the first day of work. However, over the following days the horses adapted to the work, so that the decreases in serum enzyme activities and blood lactate concentrations were reduced. Since similar observations have been described for racehorses, the determination of blood lactate concentrations and the serum activities of muscle-derived enzymes, specifically CK, seem to be good indicators of fitness in draught horses.  相似文献   

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To assess changes in 24 blood constituents in frozen serum and heparinized plasma, blood samples were drawn from 10 clinically normal German Shepherd army dogs. The storage characteristics of nine enzymes (ALP, ALT, amylase, AST, CK, GGT, GLDH, LDH, lipase), and 15 metabolites and minerals (albumin, bile acids, bilirubin, calcium, cholesterol, creatinine, fructosamine, glucose, magnesium, phosphate, potassium, protein, sodium, triglycerides, urea) were studied. Parallel samples of serum and heparinized plasma were stored for 90 and 240 days at two different storage temperatures, -200 degrees C and -700 degrees C. Sixteen of the 24 analytes (ALP, ALT, amylase, AST, CK, GGT, GLDH, LDH, bile acids, calcium, cholesterol, creatinine, fructosamine, magnesium, phosphate, urea) showed statistically significant (p < 0.05) changes during the storage period related to storage time, storage temperature, and sample type. Seven of the analytes (amylase, GGT, GLDH, LDH, bile acids, fructosamine, magnesium) showed changes of possible clinical importance with mean differences from baseline larger than 20% for the enzymes and 10% for the metabolites and minerals during the storage periods.  相似文献   

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在实际生产中根据母猪的不同生产阶段,分为后备期、间情期、妊娠期、临产期、哺乳期和空怀期等几个阶段。为便于饲养管理和提高养殖效益,应按照各个时期的生理特点和对营养成分的需求采取不同的饲喂方式和配制全价的饲料,满足母猪对营养物质的需求,能保持适宜的体况和膘情,适时发情和配种,并生产出更多更优的仔猪,提高母猪繁殖力和养殖效益。该文分别叙述不同阶段母猪的饲养管理要点。  相似文献   

5.
繁殖母羊是羊场快速扩繁的基础,饲养繁殖母羊的最终目的是充分挖掘母羊的生产潜力,增加羊群数量,提高羊群质量.母羊每年都要经历1~2次配种、妊娠、哺乳这一复杂的繁殖生理过程,每一次繁殖经历,都是对繁殖母羊体质的一次重要考验.只有不断优化繁殖母羊空怀期、妊娠期和哺乳期各阶段、各环节的饲养管理,才能保证繁殖母羊的体质健康,提高...  相似文献   

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BACKGROUND: Therapeutic use of corticosteroids has been implicated in a variety of serum biochemical abnormalities in dogs; however, the effects in cats are less well characterized. OBJECTIVE: The objective of this brief communication is to report serum biochemical changes in response to methylprednisolone acetate (MPA) in adult cats. METHODS: Serum biochemical profiles were performed on 11 cats with dermatologic diseases at baseline, 3-6 days, and 16-24 days after a single intramuscular dose of 5 mg/kg MPA. RESULTS: Median serum albumin and bicarbonate concentrations and amylase activity were increased compared to baseline at both post-treatment time points; aspartate aminotransferase activity and magnesium concentration were increased at 3-6 days post-treatment only; and alkaline phosphatase activity and total calcium concentration were increased at 16-24 days post-treatment only. Median serum creatinine concentration was decreased compared to baseline at both post-treatment time points. Examination of data from individual cats revealed significant variability in serum biochemical changes in response to MPA. No adverse clinical reactions to the drug were reported. CONCLUSIONS: A single dose of MPA results in significant changes in some serum biochemical values in cats, although individual responses will vary.  相似文献   

8.
ABSTRACT

1. Two separate experiments were conducted to evaluate the efficacy of either prebiotic or synbiotic on growth performance, carcass yield, organ weights, and serum metabolic profile of broilers under hot climatic conditions.

2. In experiment 1, 150 1-d-old Ross 308 male broiler chickens were fed dietary synbiotic (Biomin® IMBO, 0, 1 or 1.5 g kg?1 of the starter diets and 0, 0.5 or 0.75 g kg?1 of the grower diets) for 6 weeks. In experiment 2, 150 1-d-old Ross 308 male broiler chickens were fed dietary prebiotic (Mannan oligosaccharide 0, 0.5 or 1 g kg?1 of the starter diets and 0, 0.25 or 0.5 g kg?1 of the grower diets) for 6 weeks. Each treatment had 10 replicates containing 5 broiler chickens each.

3. Supplementation of synbiotic at 1 or 1.5 g kg?1 feed to broiler diets during 1–21 d and 0.5 or 0.75 g kg?1 feed, respectively, during 22–42 d improved (P < 0.01) body weight gain, feed conversion ratio (FCR), and decreased (P < 0.01) abdominal fat, serum total cholesterol, and low-density lipoprotein (LDL) cholesterol levels in experiment 1.

4. Similarly, supplementation of the prebiotic at 0.5 or 1 g kg?1 in broiler diets during 1–21 d and 0.25 or 0.50 g kg?1 feed, respectively, during 22–42 d improved (P < 0.01) body weight gain, FCR, and decreased (P < 0.01) abdominal fat, serum cholesterol, and LDL-cholesterol levels in experiment 2.  相似文献   

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Studies were conducted to determine the effects of delaying the separation of serum from the clot and of long-term storage of serum samples on the measurement of thyroid hormones in blood from horses using a fluorescence polarization immunoassay. The measured concentrations of T3 and T4 were not affected by leaving serum on the clot for as long as 24 hours at room temperatures. Storage of serum for 19 to 22 months at -20 degrees C resulted in significant increases of measured T4, but not T3. These studies support previous work demonstrating that thyroid hormones are resistant to degradation, immunologically stable, and reasonably insensitive to potential problems of routine specimen handling when measured with an immunoassay.  相似文献   

10.
复方中草药添加剂对哺乳母猪泌乳力的影响   总被引:14,自引:0,他引:14  
选用18头经产约克母猪,随机分为药物组和对照组,每组9头,用蒲公英、王不留行、漏芦、续断组成复方中草药添加剂对哺乳母猪进行20d的饲养试验。结果表明:药物组比对照组窝重提高15.04%(P<0.01),成活率提高6.03%(P<0.05),平均日增重提高6.47%(P<0.05)。  相似文献   

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The paired lateral abdominal veins (LAV) provide alternative venipuncture sites in grey nurse sharks (Carcharias taurus, Rafinesque 1810) and their efficacy was assessed using morphometrics, necropsies, ultrasound‐guided blood sampling and by comparing serum biochemistry between the LAV and caudal vein (CV) with values from the latter published previously. The mean length of the CV was 2.8% of total length (TL) whereas each LAV was 22.4% of TL and, when combined, was approximately 16 times longer than the CV. The mean tissue thickness overlying each LAV increased significantly (p < .001) with increasing TL and ranged from 3.5 to 33.8 mm in the smallest to largest shark. The mean internal diameter of the paired LAV also increased significantly (p < .001) with increasing TL and was equal to or exceeded the CV. Experienced SCUBA divers captured 56 free‐living grey nurse sharks and 46 healthy animals were sampled for blood from the LAV and CV with minimal risk to the animals or staff. Venipuncture of the LAV (n = 16) was easily accomplished using ultrasound guidance with a 38 mm/18‐gauge needle, whereas standard methods were used with the CV (n = 30). Serum biochemistry was compared (t‐tests) and none of the biochemical analytes differed significantly between the LAV and CV. The paired LAV produced representative blood samples and could also be used for fluid therapy and/or intravenous anaesthesia as has been done in other sharks. It is recommended that veterinary/husbandry staff familiarize themselves with the paired LAV and consider their use in the future.  相似文献   

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This study aimed at investigating the effects of weaning kids abruptly at an average of 55 ± 13 days of age on intake, behavioral and serum parameters and, lasted for a total of six weeks; two weeks pre-weaning and four weeks post-weaning. Sixteen single kids with equal gender were used. Kids were only allowed to stay with their mothers for suckling (45 min/period) both in the morning and in the evening period during pre-weaning. Grower concentrate and hay were offered ad libitum . The duration of the study was divided into three periods for the sampling of behavioral and serum parameters; (i) pre-weaning period lasting for two weeks (P-BW) (ii) early post-weaning period lasting for one week (P-AW1) and (iii) late post-weaning period lasting for three weeks (P-AW2). Daily weight gain of kids gradually decreased as the observation period progressed ( P  = 0.001). However concentrate feed intake increased from 0.154 kg/day in P-BW to 0.479 kg/day in P-AW1 and 0.499 kg/day in P-AW2. Water intake, rumination and standing behaviors decreased in P-AW2 ( P  < 0.001), whereas activity towards concentrate feed (CF) ( P  < 0.001) and roughage ( P  = 0.012) increased as compared to P-BW and P-AW1. Abnormal oral activity was not affected by the periods ( P  = 0.906). CF was significantly higher in females ( P  = 0.003), whereas males displayed higher lying behavior ( P  = 0.007). Glucose, total protein, urea, cholesterol, HDL-cholesterol, LDL-cholesterol concentrations ( P  = 0.001) and ALP activity ( P  = 0.003) were significantly affected by the periods. The results of the present study suggest that behavioral and serum parameters across the periods describe changes in the nutritional conditions as a result of the transition from milk to solid feed in association with weaning.  相似文献   

13.
Routine hematological and serum biochemical screening was done in 61 female dogs with benign mammary tumors and 51 female dogs with malignant mammary tumors. Most parameters were not significantly different from age-matched female controls; moreover, no significant difference could be observed between animals with benign and malignant tumors. It is concluded that routine hematology and biochemistry offer little diagnostic or prognostic benefit in female dogs with mammary tumors.  相似文献   

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随着养猪规模化、集约化发展,猪群对饲养管理水平的要求越来越高,不仅关系到猪场的健康发展,还决定整个养殖效益。尤其是提高母猪的饲养管理水平至关重要,该文主要论述不同阶段母猪饲养管理要点。  相似文献   

15.
The influence of different copper and zinc contents in rations on blood serum concentrations was tested in a feeding trial at the Institute of Animal Nutrition of the Federal Research Institute for Animal Health (FLI). A total of 60 German Holstein breed cows (38 first lactating and 22 second lactating cows) were tested. All animals received a diet based on maize‐ and grass silage ad libitum. The animals were divided into two groups with 30 cows each. Group A received concentrates supplemented with copper and zinc as recommended, whereas Group B was offered a concentrate with roughly double the amount of copper and zinc. Blood samples were taken several times during the lactation. The concentrations of copper and zinc in the serum were measured and correlations between trace element intake and their serum concentrations were calculated. The mean dry matter intake was significantly higher for cows in the second lactation (19.1 kg/day) than for cows in the first lactation (16.4 kg/day), whereas the DM‐intake was constant in the different feeding groups (17.3 and 17.4 kg/day). The correlation between feed intake in the first and the second lactation was r = 0.76. The copper concentration in the serum was not influenced by the different feedings (11.9 and 12.5 μmol/l), but high variations were found between the cows. The influence of the lactation number was not significant (12.1 and 12.4 μmol/l). In contrast to copper, the zinc concentration in the serum was significantly higher for Group B (14.0 μmol/l) than for Group A (12.2 μmol/l), respectively, for second lactating cows (13.8 μmol/l) in relation to 12.7 μmol/l for first lactating cows. The individual variation was also high as for copper. Neither the correlation between copper intake and copper serum concentration nor the correlation between zinc intake and zinc serum concentration was significant.  相似文献   

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Objective To evaluate the fertility of mares bred at various intervals relative to surgical management of rectovaginal fistula (RVF). Materials and Methods Surgical repair of RVF was performed in 28 mares at variable times relative to foaling (30 days to 24 months) and also relative to rebreeding (same cycle or delayed). Postoperative fertility was then evaluated. Results Two mares were already pregnant at the time of surgery and 20 of 23 mares (87%) that were bred immediately prior to or following surgery conceived from their first service. When mares were bred in the same cycle as surgery, the next cycle following surgery or in the following breeding season after surgery the pregnancy rate was 5/5, 5/6 and 10/12, respectively, and the foaling rates were 4/5, 4/6 and 7/12. The two mares already pregnant at the time of surgery foaled successfully. Conclusions Excellent fertility can be achieved following surgical repair of RVF and our results suggest that delaying breeding until the following breeding season is not necessary. In addition, breeding in the same cycle as the surgical repair is a previously unreported technique that should be considered to maintain normal fertility and a yearly foaling interval.  相似文献   

17.
A potentiated sulpha drug was administered intravenously to 12 sows on the 17th day of lactation and to 4 sows in early pregnancy to study the influence of lactation on its disposition kinetics. The dose-rate of sulphadoxine (SDX) used was 12 mg/kg b.w. while that of trimethoprim (TMP) was 2.4 mg/kg b.w. The pharmacokinetic parameters of SDX showed no significant difference between lactating and pregnant sows (V ss, 0.24±0.04 L/kg; Cl s , 0.25±0.05 ml/min per kg: MRT, 17.08±4.48 h). SDX did not accumulate in milk, the concentrations in milk being less than the concentrations in serum at the same time. Of the pharmacokinetic parameters for TMP, only the mean residence time was significantly different between the two groups (V ss, 1.60±0.31 L/kg; Cl s , 4.62±1.07 ml/min per kg: MRTlactating, 5.43±1.26 h; MRTpregnant, 7.74±1.72 h). TMP was excreted in milk to a considerable extent, the ratio of its concentration in milk to that in serum at the same time being over 2.2. These two substances show a completely different pharmacokinetic behaviour. Even though TMP is excreted more quickly in lactating sows, adjusting the dose of this potentiated sulpha drug does not seem to be appropriate.Abbreviations AUC area under the curve - AUMC area under the first-movement curve - terminal elimination rate constant - b.w. body weight - Cl s clearance at steady state - D dose - MRT mean residence time - SD standard deviation - SDX sulphadoxine - TMP trimethoprim - V ss apparent volume of distribution at steady state  相似文献   

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通过测定和分析自发性糖尿病猕猴的血液学、生化指标,为建立猕猴自发性糖尿病模型提供参考数据。对发生类似人类糖尿病“三多一少”症状的1只猕猴,应用全自动血细胞分析仪和血液生化分析仪,测定其血液学、生化值,并进行糖耐量试验。结果血糖(空腹)、尿糖和尿素显著升高;甘油三酯、肌酐、总胆固醇、氯、磷、镁和二氧化碳处于正常值高限或增多,血液学中性粒细胞百分比、血红蛋白、平均红细胞血红蛋白和平均红细胞血红蛋白浓度升高。表明猕猴为典型的自发性糖尿病动物。  相似文献   

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为研究不同水平丁酸梭菌对肉鸡生长性能、血清生化指标及经济效益的影响,选择2 000只体重相近、健康的1日龄AA肉仔鸡,随机分为5组,每组4个重复,每个重复100只鸡.空白对照组肉鸡饲喂基础饲粮,抗生素对照组在基础饲粮中添加100 mg/kg金霉素,试验1、2、3组分别在基础饲粮中添加100、400、600 g/t丁酸梭...  相似文献   

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