Predictive Markers for Cold-Induced Sweetening Resistance in Cold Stored Potatoes (<Emphasis Type="Italic">Solanum tuberosum</Emphasis> L.)

An approach has been developed to screen a large number of potato clones for cold induced sweetening (CIS) resistance in breeding programs. Two key enzymes responsible for reducing sugar accumulation during cold storage were identified. Clones with the A-II isozymes of UDP-glucose pyrophosphorylase coupled with low activity of vacuolar acid invertase enzyme had increased resistance to CIS by forming less suc, which is subsequently hydrolyzed to the undesirable reducing sugars, glc and fru. Six named cultivars and 192 genetically diverse clones from various breeding programs in USA were analyzed over two years for the two key enzymes and sugar concentration in cold stored tubers. The predictability for CIS resistance during cold storage was 94% both years. Clones classified as class A accumulated low concentration of reducing sugar glc during cold storage. It is suggested that these two predictor enzymes can be used for screening parents and selections in potato breeding program.     

Evaluation in pot and field trials of resistance of potato cultivars and breeding lines to common scab caused byStreptomyces scabies

Summary The resistance to common scab of nine cultivars and eight advanced breeding lines of potatoes, fiveSolanum andigenum Juz. et Buk. clones, and three potato breeding lines selected for resistance to common scab, was investigated in a field and a pot trial. In the pot trial potaroes were planted in inoculated coarse river sand in plastic pots (150 mm in diameter) placed on irrigated subsoil in a glassfibre house. In a field trial potatoes were planted in an artificially infested field. The harvested tubers were scored individually for scab incidence according to two scales, viz. a 1–5 point scale for percentage surface covered with scab and a 1–3 point scale for lesion type. A scab index value was calculated for each tuber. A significant correlation coefficient of 0.82 was obtained for the results of the two tests.     

A conservation-tillage, cover-cropping strategy and economic analysis for creamer potato production

A two-year experiment was conducted at the Beltsville Agricultural Research Center (BARC), MD, and Virginia Polytechnic Institute and State University Kentland Agricultural Research Farm (KARF), VA, to evaluate potato (Solanum tuberosum L.) production of 32- to 57-mm-size-range tubers (referred to hereafter as creamers) in a conservation-tillage, cover-cropping strategy. The experiments used a split-plot design in which the main-plots were cover crop treatments and the sub-plots were different potato selections. Main plot treatments included rye (Secale cereale L.), crimson clover (Trifolium incarnatum L.), Austrian winter pea (Pisum sativum L.), rape (Brassica napus L.), oat (Avena sativa L.), rye/crimson clover mixture, rape/crimson clover mixture, bare soil/raised beds, and bare soil/flat beds (control). Potato selections tested were B1145-2, B1491-5, and B1492-12 in 2000 and B1145-2, B1102-3, and B0811-4 in 2001. Yields in the conservation-tillage treatments were equal to or better than those in the bare soil/flat bed control with few exceptions. Large-sized tubers (<57 mm) in almost all cases remained below 6% of total marketable yield even when the tubers were harvested late. Delayed harvest did not reduce creamer-sized yields nor did it increase yield of large-sized tubers. Economic analysis shows that net returns from some conservation-tillage treatments are equal to or higher than the conventional-tillage strategy and confirms the viability of the conservation-tillage, cover-cropping strategy. Furthermore, the conservation-tillage strategy in many cases allows timely planting using machinery in the wet soils of Maryland and Virginia during the narrow window of spring potato planting time, whereas the conventional tillage strategy does not offer this advantage.     

Comparison of seasonal development of tuber specific gravity among seven potato clones

Specific gravity of potato tubers provides an estimate of starch content of which uniformly high levels are important for product quality. The objectives of this study were to document the relationship between the level of specific gravity and the variability among tubers and to model the seasonal development o f specific gravity for seven potato clones. Individual determinations were made on twenty tubers from each plot using the weight-in-air, weight-in-water method on each of eight harvest dates spanning the tuber growth period. For each plot, the mean and standard deviation of specific gravity were calculated and used to define the correlation between specific gravity level and tuber-to-tuber variability and to construct a predictive model of the seasonal specific gravity developmental pattern. As the season progressed, tuber-to-tuber variability of specific gravity increased for some clones but not for others. A positive correlation existed between tuber specific gravity mean and its standard deviation for four of the seven clones in 1991 and five in 1992. Correlation coefficients were generally low, suggesting only a weak association between specific gravity and its standard deviation. Tuber specific gravity developed in a similar seasonal pattern for the seven clones. It generally started low, reached a maximum approximately seven to eleven weeks after tuberset, at which time some clones showed a tendency to decline through the remainder of the season. Quadratic regression equations provided an adequate model to describe specific gravity development for all seven clones over the tuber growth period. Statistical contrasts were used to test for differences in equation parameters, including line coincidence, line slope, and intercept. The model showed that initial specific gravity level at tuber set, the rate of early season increase, as well as the length of time from tuber set to specific gravity maximum, varied by clone and determined the level of specific gravity at season’s end.     

A plot design to sequentially harvest early generation selections and its use in evaluating fresh market characteristics of three new cultivars

A plot design was developed to compare changing yield and quality characteristics of early generation selections in our potato breeding program over a 6-wk harvest season. In this design all replicates for one harvest date are located within one row to facilitate harvesting and the variety plot arrangements in adjacent rows are identical to eliminate inter-variety plant competition effects. To illustrate the usefulness of this design, three new fresh market cultivars, Norkotah Russet, HiLite Russet, and Frontier Russet, and a standard commercial cultivar, Norgold Russet, were harvested every 10 to 14 days from late July through early September to measure earliness and optimum time of harvest for each cultivar. Each cultivar was found to have a characteristic maturation pattern. Total and marketable yields and tuber size distribution generally improved in all four cultivars as the harvest season progressed. However, the yield of marketable tubers of Norkotah and Frontier peaked and then decreased later in the season as many tubers became oversized (over 340 gms). Tuber number per plot was a stable attribute and was judged to be the most important factor determining performance of each line. Norkotah and Frontier produced fewer tubers and showed rapid tuber bulking and early production of marketable tubers of preferred sizes. HiLite produced more tubers than the other cultivars so had higher percentages of undersized tubers in late July and early August, but it produced high yields and desirable size distributions in late August and early September. Specific gravities for all three new cultivars decreased during early August, increased in mid-August as plants died, and then declined. HiLite and Frontier had higher solids than Norgold and Norkotah throughout the season.     

Phenotypic stability of resistance to late blight in potato clones evaluated at eight sites in the United States

Changes in the fungal pathogenPhytophthora infestans in the United States pose a significant threat to potato production. Sources of resistance to these new genotypes of P.infestans need to be identified for potato breeders to have parental materials for crossing, and the phenotypic stability of late blight resistance in these potato clones needs to be determined. Sixteen potato clones which reportedly have some resistance to late blight were evaluated at eight locations: Florida (FL), Maine (ME), Michigan (MI), Minnesota (MN), North Dakota (ND), New York (NY), Pennsylvania (PA) and Wisconsin (WI) in 1996. Percent infected foliage was recorded at approximately weekly intervals following the onset of the disease at each location. Area under the disease progress curve (AUDPC) was calculated. Clones were ranked for mean AUDPC within location and the nonparametric stability statistics, mean absolute rank differences and variance of the ranks, were analyzed for phenotypic stability. Neither of these statistics was significant, indicating a lack of genotype x environment interaction on the rankings of these clones across locations in 1996. The four clones with lowest AUDPC scores were U.S. clones AWN86514-2, B0692-4, B0718-3 and B0767-2. These clones should be useful parental materials for breeders seeking to incorporate genes for late blight resistance into potatoes.     

Total protein and protein fractions in tubers of Group Andigena and Phureja-Tuberosum hybrids

Proteins of potato clones with a wide range of total protein content and from two different genetic backgrounds were divided into albumin, globulin, prolamin, glutelin and residual fractions. More soluble protein was recovered from older, more mature tubers than was recovered from younger tubers. Differences in amounts of the protein fractions were detected among the clones. However, the amount of each fraction within a clone was highly correlated with total protein. The percentage of the total protein allocated to each fraction remained relatively constant among the clones which are assumed to be producing mature tubers. Soluble protein (albumin plus globulin) correlated highly with total protein in both Andigena and Phureja-Tuberosum' selections.Scientific Journal Series Article 11, 153 of the Minnesota Agricultural Experiment Station. This research was supported by a grant from the Rockefeller Foundation.     

Selection for resistance to potato leafhopper in potatoes I.selection methods

A randomly mated potato (Solarium tuberosum L.) population (3 generations, without selection) derived from clones from several breeding programs, displayed considerable resistance in the field to potato leafhopper,Empoasca fabae (Harris). Leafhopper population levels on 161 clones ranged from 2 to 28 nymphs per 45-sec count (mean = 12), whereas a susceptible check averaged 20 nymphs per count. Clones selected for low leafhopper counts in 1971 were compared with the population in 1972 to evaluate the effectiveness of selecting from small plots (three-hill) with minimum replication. The selected clones averaged 30% fewer leafhoppers than the population average in 1971 and 10% fewer in 1972. The average heritability (broad sense) for one plot per clone per location, two locations, and two 45-sec nymph counts per plot equaled 36%.     

Assessment of latent infection frequency in progeny tubers of advanced potatoclones resistant to bacterial wilt: A new selection criterion

Summary This paper reports results of a 3-year evaluation of CIP advanced potato clones in a bacterial wilt-infested field (race 3) in Peru. Clones resistant or moderately resistant to wilt were selected and all tubers harvested from each clone were tested for latent infection byRalstonia solanacearum using a sensitive serological technique developed at CIP. A sampling strategy to estimate accurately the frequency of infected tubers in the clones has been evaluated. This method will allow consideration of tuber latent infection as a new selection criterion in breeding for resistance to bacterial wilt. Thirteen clones were found resistant to wilt in all three evaluations (i.e.≤6% wilt), from which five had no wilt in all trials. However, all clones harboured latent infection in tubers averaging 30%. Analysing 30 tubers/clone provides an accurate estimation of the proportion of infected tubers with a high precision level.     

The germplasm release of B0718-3 and B0767-2: Two late blight resistant potato clones

Two potato breeding selections were released by the United States Department of Agriculture, Agricultural Research Service on November 14, 1996, as germplasm that is highly resistant to current populations ofPhytophthora infestans (Mont.) deBary, the causal organism of potato late blight: B0718-3 and B0767-2. These two clones have been evaluated for their reaction toP. infestans in replicated plots at Presque Isle, Maine since 1990. B0718-3 is a netted white-skinned selection with good yields but low specific gravity. It is both male and female fertile and crosses readily with tetraploid Tuberosum. B0767-2 is a russet-skinned selection that is low yielding and low in specific gravity. It has not been successfully crossed; however, it may have some potential use in somatic hybridizations. Neither B0718-3 nor B0767-2 process into acceptable colored chips. Genetic material of this release has been deposited in the National Plant Germplasm System where it is available for research purposes, including development and commercialization of new cultivars.     

~(60)Coγ射线对马铃薯的辐射效应及其在育种中应用的研究

本试验系不同剂量和剂量率的γ射线照射不同品种马铃薯的休眠块茎,诱发出突变株系,选育出高产、抗病、优质的辐射高原7号等新品系用于生产,获得了显著的增产效果。本文通过对各辐射株系与原品种主要遗传性状的观察、比较,对马铃薯辐射育种中的几个遗传变异问题进行了初步探讨。     

Comparison of Russet Burbank clones from various geographical regions of the United States and Canada

Ten ‘Russet Burbank’ potato clones collected from various geographical regions of the United States and Canada were evaluated for plant and agronomic characteristics for two years at Aberdeen, Idaho. Clonal differences were detected for emergence time, stem number per hill, expression of Verticillium symptoms, some measures of yield, and percentage of tubers with hollow heart. Eight of the ten clones were quite similar. Differences were slight or found only in a few characteristics. One clone, collected from northern Idaho, was similar to the homogenous eight clones in all respects except yield, which was probably due to chronic PVX infection throughout the trial period. Another clone, collected from New Brunswick, was distinct from all other clones and was markedly inferior with respect to many of the characteristics measured when grown in Idaho.     

Performance of true potato seed families. 2. Comparison of transplants vs. seedling tubers

Summary A potato crop can be grown from true potato seed (TPS) either by transplanting seedlings to the field (seedling transplants) or by planting tubers derived from seedlings (seedling tubers). The effect of the planting method on performance of TPS families was evaluated for three types of families, 4x×2x hybrid, open pollinated, and self pollinated. Families from seedling transplants and seedling tubers and their 4x parental clones were compared for tuber yield and specific gravity. Families from tubers had substantially higher yields and significantly more uniform specific gravity than families from transplants. Hybrid seedling tuber families had a higher mean yield than the parental clones and did not differ from them in specific gravity. Selection in the seedling generation could further improve both OP and hybrid families from transplants and from seedling tubers. Paper no. 3163 from the Department of Genetics, University of Madison.     

Potato chip color ratings of advanced selections from the Maine potato breeding program

Seven named varieties and three numbered selections from the University of Maine potato breeding program were compared for chip color at various conditions and lengths of storage. Although all selections tested were equal to or better than Kennebec in chip color, only Allagash Russet equaled Monona. Storage at 45° F (7.2° C) produced acceptable chip color at all time periods tested. After long term storage at 45° F, reconditioning at 55° F(12.8° C) was ineffective in improving chip color. Following storage at 38° F (3.3° C) acceptable chip color could be produced only by reconditioning at 70° F (21.1° C) for at least two weeks.     

Enzymatic discoloration and phenolic content of potato tubers from cultivars resistant and susceptible to the golden nematode

Phenolic content and discoloration were determined for 13 commercial potato cultivars and 6 potato clones grown at 2 locations over a period of 5 successive years. Tubers from 6 potato cultivars and 5 potato clones resistant to the golden nematode were compared with tubers from 7 potato cultivars and 1 clone known to be susceptible to the golden nematode. Tubers from resistant plants were lower in phenols and discolored less than tubers from susceptible plants.     

Partial collection of data on potato yield for experimental planning

The aim of this study was to estimate the number of blank experiments (BE) (i.e., a uniformity trial) required to estimate the optimum plot size for use in experiments involving potato crops. The study was based on data on the mass of potato tubers (Solanum tuberosum L.) from 3456 hills (i.e., 24 rows of 144 hills each) obtained from a BE. Using these data, BE of different sizes (i.e., 2 rows of 24, 36, 48 and 72 hills) were planned to estimate optimum plot size. For each BE, 11 plot sizes (X) were planned based on the sum of adjacent hills, and the mean, variance and coefficient of variation (CV) between plots of the same size were calculated. Regression models for CV were adjusted in terms of X to estimate the optimum plot size. For each BE size, a bootstrap resampling method was used to estimate the sufficient number of BE to enable precise estimates of optimum plot size, mean and other statistics. It was found that a sampling potato hill yield of 39% of subdivisions within an experimental area where a potato experiment is to be performed is sufficient to estimate optimum plot size for the experiment. Plots composed of one row of six hills are sufficient to estimate potato yield.     

Effect of potato seed tuber glycoalkaloid content on subsequent infection byRhizoctonia solani

Since glycoalkaloids have been shown to have fungitoxic properties, it is important to know what impact this might have on potato plant breeding programs which select for low levels of total glycoalkaloids (TGA) in the tuber. Potato clones with TGA levels ranging between 1.6 and 32.8 mg/100 g fresh tissue were planted in two trials to evaluate the relationship between seed tuber TGA and incidence ofRhizoctonia solani infection in the developing plant. An inoculation procedure was followed in the greenhouse and field to establish uniform infection of potato seedlings. Plants were examined for lesion development on stems and stolons and assigned disease ratings. In the field, yield of malformed tubers was recorded and included in the overall disease ratings. The results reported herein indicate that TGA levels in the seed tuber are unrelated to the severity ofR. solani infection in the seedling.     

Verticillium wilt resistant potato germplasm: A66107-51 and A68113-4

Potato breeding selections A66107-51 and A68113-4 (Solanum tuberosum gptuberosum) are being released as germplasm that is highly resistant to Verticillium wilt (Verticillium dahliae Kleb.) These selections have been extensively evaluated for resistance to Verticillium as measured by symptom severity and degree of colonization byV. dahliae. They combine outstanding wilt resistance with good tuber maturity characteristics, high yield, and french fry processing tuber type. A66107-51 has long-oblong, russeted tubers, with high yield and variable processing quality. A68113-4 has long-oblong, partially russeted tubers with high yield and good processing quality. Both selections are fertile as female parents, and A66107-51 is occasionally pollen fertile. The Verticillium resistance of these two clones has been transmitted to progeny. These selections were developed by USDA-ARS in cooperation with the Idaho Agricultural Experiment Station. Seed is available from USDA-ARS, Aberdeen, Idaho.     

Variation of Growth and Disease Characters Between Clones of Potato (<Emphasis Type="Italic">Solanum tuberosum</Emphasis> L.)

The occurrence and magnitude of variation of selected characters in clones derived from meristem tips of four potato cultivars in field trials in three countries was assessed. Recorded were: the number of plants per plot; maturity of plants; skin and flesh colour; tuber deformity; time of emergence; flowering; number of stems and tubers per plant; tuber size, yield and dry matter percentage; and the occurrence of common scab. Far more significant differences between clones than could be expected by chance were found for the last eight characters. Most of the significant differences for number of stems per plant, number of tubers, yield and tuber size distribution were found in six clones of three cultivars and were repeated at different locations and in different years. It is concluded that significant clone differences exist and are repeated at independent locations over the years.     

Resistance to late blight and soft rot in six potato progenies and glycoalkaloid contents in the tubers

Glycoalkaloids are anti-nutritional compounds commonly found in wildSolanum species used as resistance sources to major potato pathogens. It is therefore important for breeding purposes to know whether selecting for resistance using such species necessarily selects also for high glycoalkaloid contents in the tubers. To test this hypothesis, we used six partial progenies from crosses betweenSolanum tuberosum and accessions ofS. andigena, S. berthaultii, S. phureja, andS. vernei to investigate the possible correlation between resistance toPhytophthora infestans and/or toErwinia carotovora subsp.atroseptica and the concentration of glycoalkaloids in tubers. Concentrations of α-solanine and α-chaconine in the tubers segregated in each progeny, as did resistance to each pathogen. Some, but not all, clones from each progeny showed hypersensitive reactions to the isolate ofP. infestans used. Furthermore, clones within each progeny also differed for components of partial resistance toP. infestans, suggesting that all four wild species could be used as sources of both race-specific and partial resistance to late blight. With the exception of low, but statistically significant, correlations between concentration of α-solanine and two late blight resistance components (incubation period and spore production per unit lesion area) in progenies derived fromS. vernei, and despite a trend towards higher glycoalkaloid concentrations in the tubers of the clones most resistant to soft rot within progenies derived fromS. berthaultii andS. vernei, no consistent relationship between resistance to either disease and concentrations of α-solanine and/or α-chaconine was observed. These results indicate that neither race-specific nor partial resistance to late blight and soft rot in the accessions used as progenitors of resistance depend on high solanine or chaconine concentrations. These resistance sources could thus prove useful in breeding programs for improved behaviour againstP. infestans and/orE. carotovora.