鸡大肠杆菌病的鉴别诊断

1鸡大肠杆菌病概述 鸡大肠杆菌病是由埃希氏大肠杆菌的各种血清型菌株引起疾病的总称。大肠杆菌病在临床上有多种症状,如大肠杆菌性败血症、肉芽肿、腹膜炎、输卵管炎、脐炎、滑膜炎、气囊炎、全眼球眼炎、卵黄性腹膜炎等症状。     

成都地区鸡大肠杆菌病病原分离鉴定及血清型调查

本试验从成都及毗邻十二个县（市）部分养鸡场（户）送检的疑似鸡大肠杆菌病的病鸡中分离出的５４３株鸡致病性大肠埃希氏病,取其中６９株进行血清型鉴定,其血清型分别为Ｏ７８：Ｋ８０,Ｏ５５,Ｏ２,Ｋ３,Ｏ８：Ｋ４０,Ｏ１２５：Ｋ７０,Ｏ１：Ｋ１,Ｏ２６：Ｋ６０,Ｏ１４２：Ｋ８６,Ｏ２９,Ｋ１２７ａ：Ｋ６３及Ｏ１６４。其中Ｏ１２５：Ｋ７０,Ｏ１４２：Ｋ８６及Ｏ１２７ａ：Ｋ６３血清型的Ｅ,Ｃｏｌｉ使鸡致病的剖     

鸡大肠杆菌病的诊断与防治对策

大肠杆菌病是由大肠埃希氏菌的某些血清型引起的，可引起鸡胚胎死亡，或雏鸡和幼鸡死亡率很高的败血症，以及脐带炎、关节炎、眼球炎、出败性肠炎及大肠杆菌性肉芽等多种病型的疫病，是危害养禽业的一种严重的传染病。近年来由于养禽业的快速发展，该病在一些鸡场中严重发生，带来的损失愈显突出。     

禽大肠杆菌病的预防与治疗

禽大肠杆菌病是由致病性大肠埃希氏菌引起禽类的急性、慢性传染病的总称。其病型和病变复杂多样,主要有急性败血症、卵黄性腹膜炎、脐炎、输卵管炎、气囊炎、肠炎、全眼球炎及大肠杆菌性肉芽肿等。本菌抗原结构复杂,血清多型,变异菌株不断出现,分布极广,不同地区有不同血清型,同一地区不同鸡场甚至同一鸡场同一鸡群也可有多个血清型,本病的普遍性,给养禽业造成严重威胁和重大经济损失。     

鸡大肠杆菌病的防治

大肠杆菌病是由致病血清型或条件致病性大肠埃希氏杆菌引起的局部或全身性感染的各种禽类的急性或慢性疾病的总称,包括大肠杆菌性败血症、气囊炎、肝周炎、腹膜炎、输卵管炎等等。由于集约化养鸡业的发展,加上并发和继发其他疾病,临床症状复杂,致使鸡大肠杆菌病已成为当前养鸡业最主要及最常见的疾病之一。     

鸡大肠杆菌病多发的原因分析及防治

鸡大肠杆菌病是由某些致病性血清型大肠埃希氏杆菌（E．Coil）引起的鸡的不同类型疾病的总称。特征是引起鸡的心包炎、肝周炎、气囊炎、腹膜炎、眼球炎、关节炎及滑膜炎、输卵管炎、大肠杆菌性肠炎、肉芽肿、败血症等，该病1945年在瑞典被首次报道，以后在世界上许多国家陆续发生。     

如东地区鸡源性大肠杆菌分离株血清型与耐药性分析

禽大肠杆菌病是由大肠埃希氏杆菌引起的鸡的一种最为常见的细菌性传染病,其抗原性复杂,血清型多样,临床症状和病理变化多变,且易与病毒性疾病和其他细菌性疾病并发,给养鸡业构成了严重威     

鸡大肠杆菌病的病因与实验室诊断

大肠杆菌病是由埃希氏大肠杆菌引起的常见病,包括大肠杆菌性肉芽肿、腹膜炎、输卵管炎、脐炎等疾病,对养禽业危害严重。由禽类分离获得的大肠杆菌血清型只对禽类有致病力,但其中也有少数与其他动物疾病有关。剖检症状有纤维素性腹膜炎、心包炎、眼炎、生殖器官。     

鸡大肠杆菌的防治

鸡大肠杆菌病是由某些血清型的致病性大肠埃希氏菌引起的疾病。是现代养鸡业最主要疾病之一。鸡发生大肠杆菌病时出现多种病型,主要有急性败血症、气囊炎、脐带炎、眼球炎、关节炎、卵黄性腹膜炎、脑炎以及大肠杆菌性肉芽肿,致病性大肠杆菌还可以穿过蛋壳引起鸡胚感染,造成死胚率增加,孵化率下降和弱雏率增加,是严重危害养鸡业的重要疾病之一。     

The Pho regulon and the pathogenesis of Escherichia coli

During the course of infection, bacteria must coordinately regulate gene expression in response to environmental stimuli. The phosphate (Pho) regulon is controlled by the two component-regulatory system PhoBR. PhoBR is activated during starvation and regulates genes involved in phosphate homeostasis. Several studies have highlighted the importance of the Pho regulon in bacterial pathogenesis, showing how induction of PhoBR, in addition to regulating genes participating in phosphate metabolism, leads to modulation of many cellular processes. The pleiotropic effects of Pho regulon activation include attenuated virulence and alteration of many virulence traits, including adhesion to host cells and resistance to cationic antimicrobial peptides, acidity and oxidative stresses. This review provides an overview of the relationship between the Pho regulon and virulence in Escherichia coli and illustrates that, in addition to regulating phosphate homeostasis, the Pho regulon plays a key role in regulating stress responses and virulence.     

微生态制剂对鸡体内大肠埃希氏菌的拮抗试验

试验比较了微生态制剂和喹乙醇对鸡体内大肠埃希氏菌的拮抗效果。结果表明，微生态制剂用于预防鸡大肠杆菌病的效果理想，但发病时的疗效却不及药物治疗效果。     

复方白头翁颗粒对人工感染鸡大肠杆菌病疗效试验

本试验采用人工诱发鸡大肠杆菌病的病理模型,进行复方白头翁颗粒对鸡大肠杆菌病的疗效研究。结果表明:复方白头翁颗粒高剂量组(4.0 g/L)、中剂量组(2.0 g/L)对人工诱发鸡大肠杆菌病的防治效果较好,有效率分别为83.33%、76.67%,与复方磺胺间甲氧嘧啶钠可溶性粉疗效相当。     

Acid-induced autoagglutination found in chicken pathogenic Escherichia coli strain.

Chicken pathogenic Escherichia coli strains were found to autoagglutinate in a static culture of trypticase soy broth (TSB). One strain, designated PDI-386, was further studied for its autoagglutinating property. Acidity in the cultured medium caused by glucose degradation induced the autoagglutination. The bacterial cells grown in a glucose-free L-broth could be aggregated by adding acid, which suggests a potentiality of autoagglutination of the strain grown in the L-broth. The autoagglutinating parent (Agg) formed small colonies with irregular edges like rough colonies on the TS agar, whereas its non-autoagglutinating variant (Nag) formed larger smooth colonies with a perfectly round edge. The Nag colony was easily generated from the Agg colony on the TS agar. The autoagglutinating property was very unstable when the bacteria was passed in the TSB, but rather stable in the L-broth. Under electron microscope, the Agg were found to possess pili of more than 20 microns in length. However, the phenotypic expression of autoagglutination did not correlate with that of mannose-sensitive hemagglutination against guinea pig erythrocytes. Incubation of the Nag in the L-broth at room temperature for more than 10 days provoked the reversion of the autoagglutination. There was no difference between the Agg and the Nag in terms of surface hydrophobicity, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns of membrane proteins and LPS, and plasmid profiles. The virulence of the Agg was higher than that of the Nag. The autoagglutination property is, however, so unstable that the pathogenicity of E. coli isolates from chickens should be carefully evaluated.     

鸡源大肠杆菌对17种抗菌药的耐药性测定

对陕西省关中主要养鸡地区分离的20株致病性大肠杆菌进行17种抗菌药的药敏实验。结果表明，除氟哌酸（NOR）、头孢哌酮（CFP）和头孢三嗪（CRO）未产生耐药性外，分离菌株对其余14种抗菌药均有程度不同的耐药。红霉素（ERY）、苯唑青霉素（OXA）和头孢呋新（CFX）普遍产生耐药性。20株菌中有14种耐药谱型，多数为6重以上联合耐药。     

我国猪源和鸡源大肠杆菌抗生素耐药性相关基因研究进展

大肠杆菌作为一种条件致病菌和重要的耐药性指示菌,国内外已有大量猪源、鸡源大肠杆菌耐药性相关基因的研究报道.受动物种属、地域等来源性差异,以及耐药机制、亚型等因素的影响,研究中涉及很多种不同的耐药性相关基因,且检出率也不尽相同.根据耐受抗菌药物种类,对这些基因作用机理及分布情况等方面综述,以期为研究大肠杆菌耐药性产生机制...     

皖北地区鸡致病性大肠杆菌的分离鉴定及药敏实验

从皖北地区10个县市42个鸡场送检的的病料中,分离获得34株大肠杆菌,根据血清学鉴定结果,共有10个血清型,以O2、O78、O26、O22、O1为主。药敏实验结果表明,所分离的大肠杆菌对强力霉素、氟苯尼考、丁胺卡那霉素、培氟沙星高度敏感。     

Persistence of cellulitis-associated Escherichia coli DNA fingerprints in successive broiler chicken flocks

Avian cellulitis in broiler chickens is primarily caused by Escherichia coli. Previous research found that the E. coli isolates of cellulitis origin were unique to each ranch, suggesting that these E. coli were endemic within the ranch environment. To test the hypothesis that the E. coli associated with cellulitis are endemic in the litter of the broiler house, we designed a study to determine whether E. coli DNA fingerprints associated with cellulitis persist over successive flocks that are grown in the same house. In addition, we assessed the impact of different cleaning and disinfection strategies on this persistence. Two broiler houses were followed on each of five farms over 3-4 flocks. A total of 353 E. coli isolates from cellulitis lesions were analyzed in this study, and 314 of these isolates (89%) were DNA fingerprinted by PFGE. In each ranch, there were several DNA fingerprint patterns that were present over successive flocks, regardless of the cleaning and disinfection strategy utilized. Isolates persisted as long as 191 days, implying that these E. coli are capable of persisting in the broiler house environment for long periods of time. In addition, these E. coli isolates were associated with cellulitis lesions in successive flocks. Thus, the isolates of E. coli that are associated with cellulitis in broiler chickens appear to be endemic in the litter environment of the broiler house.     

鸡致病性大肠杆菌Ⅰ型菌毛的研究进展

鸡致病性大肠杆菌Ⅰ型菌毛是鸡大肠杆菌病的重要致病因子.本文对其生物学特性、在感染中的作用、分子生物学等8个不同方面的研究进展做了综述.