Changes of the functional diversity of soil microbial community during the colonization of abandoned grassland by a forest

The impact of secondary succession of grassland communities towards a Norway spruce forest on soil microbial community was studied on a belt transect established in the Pol’ana Mts., Central Europe. Data on understory vegetation, light availability, soil properties and microbial activity were collected on 147 plots distributed over regular grid. Moreover, distributions of functional groups of microorganisms were assessed using BIOLOG analysis on a subset of 27 plots. Mantel partial correlations between microbial community indicators and environmental variables showed that microbial activity generally decreased with increasing tree density and size, whereas it increased with increasing radiation at the soil surface, soil temperature, and cover and diversity of understory vegetation. Functional richness and diversity of microorganisms were positively correlated with solar radiation, but also with plant species richness and diversity. Abundance of several functional groups correlated closely with succession-related variables. Redundance analysis of microbial data provided slightly different outcomes. Forward selection yielded only two environmental variables significantly influencing the composition of the microbial community: tree influence potential and organic carbon content. Abundances of several functional microbial groups correlated with tree influence, documenting that microbial community changes are at least partially driven by the colonization of grassland by trees. Nevertheless, the relative importance of abiotic environment change and plant community succession on microbial community dynamics remains unresolved.     

北江干流河岸带不同植被类型植物物种多样性分析

以北江干流河岸带4种典型植被类型(竹林、草地、混交林和桉树林)为研究对象,采用样方法调查分析其群落特征和物种多样性,运用冗余分析(RDA)探讨北江河岸带植物物种多样性与环境因子之间的关系。结果表明:北江河岸带植物物种受热带季风气候影响,植物种类繁多,共调查到59科116属136种植物,群落科属组成分散;4种植被类型的物种丰富度、Shannon-Wiener多样性和均匀度指数变化规律一致,均以混交林最高,竹林次之,桉树林最低,但各植被类型间差异并不显著,说明北江河岸带不同植被类型维持物种多样性的异质性较小,其作用更多体现在为不同物种提供栖息地;RDA结果显示,植物物种丰富度、均匀度和多样性指数与乔木层高度、草本层盖度和海拔均呈正相关,而与至河流距离和土壤含水量呈负相关,在选取的7个环境因子中,海拔对北江河岸带植物物种多样性的影响最大,土壤含水量影响次之,至河流距离影响最小。     

嘉陵江流域不同土地利用类型土壤微生物功能多样性特征

基于2013—2015年对嘉陵江流域中游（四川段）不同土地利用类型（混交林、草甸、针叶林、阔叶林、灌丛和裸地）土壤生境的调查分析数据,利用Biolog微平板法和磷脂脂肪酸甲酯法（FAMEs）系统研究了土壤微生物多样性群落特征。不同土地利用类型土壤养分和有效养分基本表现为混交林 > 阔叶林 > 针叶林 > 灌丛 > 草甸 > 裸地。不同土地利用类型土壤微生物群落代谢平均颜色变化率（AWCD）随培养时间延长而逐渐增加,土壤微生物群落代谢活性依次是混交林 > 阔叶林 > 针叶林 > 灌丛 > 草甸 > 裸地。土壤微生物对不同种类碳源的利用强度存在较大差异,碳水化合物和羧酸类碳源是不同土地利用类型土壤微生物的主要碳源,其次为氨基酸类、酚酸类和聚合物类,胺类碳源的利用率最小。土壤微生物群落的物种丰富度指数（H）、均匀度指数（E）、优势度指数（D_s）和碳源利用丰富度指数（S）总体趋势为混交林最高,针叶林和阔叶林次之,裸地最低,优势度指数在不同土地利用类型差异并不显著（p > 0.05）。主成分分析结果表明,从31个因素中提取的与碳源利用相关的主成分1,主成分2分别能解释变量方差的65.154%和81.047%,在主成分分离中起主要贡献作用的是胺类和氨基酸类碳源;土壤微生物多样性指数与土壤养分之间呈正相关,与pH值呈负相关,而土壤全碳和全氮含量对土壤微生物多样性贡献较大,这是造成土壤微生物群落功能多样性差异的主要原因。H,E和S与土壤养分各指标的相关系数绝对值均高于D_s,说明了土壤养分对土壤微生物群落优势度指数的影响作用较小。     

退化喀斯特植被恢复对土壤微生物数量及群落功能多样性的影响

采集不同恢复阶段的土壤样品,采用微生物培养法研究了退化喀斯特植被恢复对土壤微生物数量、群落功能多样性的影响。研究结果表明随着退化喀斯特植被的恢复,土壤微生物数量增加,表现为乔木群落阶段灌木群落阶段草本群落阶段裸地阶段。土壤微生物群落代谢功能分析表明:植被恢复往往导致较高的平均颜色变化率、物种丰富度和功能多样性。乔木群落阶段的平均诱导底物利用率最高,明显地与其他3个恢复阶段不同。总之,植被恢复使得土壤微生物数量增加,碳源平均利用率增强。因此,创造了更好的土壤条件更有利于退化喀斯特植被的恢复。     

赤水河流域典型植被类型的土壤微生物群落结构与多样性

土壤微生物是反映土壤环境质量的重要指标,为明确赤水河流域典型植被类型土壤微生物群落特征及优势菌属,为生态系统的恢复与管理提供理论依据,采用高通量测序技术研究了赤水河流域的5种典型植被类型(灌丛、针阔混交林、常绿阔叶林、杉木林和竹林)土壤的微生物群落结构及多样性,并探讨了其主要影响因子。结果表明:(1)不同植被类型的细菌和真菌丰富度Chao1指数差异均不显著,说明二者所观测到的物种总数没有差异。Shannon指数显示各植被类型微生物多样性存在一定差异,细菌多样性以竹林最低,显著性低于灌丛和针阔混交林(p<0.05); 真菌多样性以灌丛和杉木林显著性高于其他3种植被类型(p<0.05)。(2)5种植被类型土壤细菌优势门(相对丰度>10%)主要有变形菌门(Proteobacteria)、放线菌门(Actinobacteriota)和酸杆菌门(Acidobacteriota)。其中变形菌门(Proteobacteria)在灌丛、针阔混交林和常绿阔叶林中占绝对优势(相对丰度为29.70%～33.62%),而放线菌门(Actinobacteriota)则在杉木林和竹林中最为丰富,相对丰度占比分别为32.88%,29.88%。各植被类型土壤真菌门以子囊菌门(Ascomycota)为绝对优势菌群(相对丰度>49%)。(3)5种植被类型土壤细菌和真菌中优势菌属差异较大。在细菌属水平上,针阔混交林和竹林分别以未定名的Vicinamibacterales和芽孢杆菌属(Bacillus)为优势,而其他3种植被类型则以节杆菌属(Arthrobacter)最丰富。在真菌属水平上,灌丛和常绿阔叶林中优势关键属为子囊菌门(Ascomycota)未分类真菌属(unclassified_p_Ascomycota),杉木林以被孢霉属(Mortierella)最丰富,而针阔混交林和竹林中均以沙蜥属(Saitozyma)相对丰度最高。(4)NMDS分析表明,土壤细菌与真菌群落空间分布差异显著。(5)冗余分析表明,土壤含水量、pH值和TN(总氮)对土壤细菌群落结构具有显著性的影响,而pH值、容重、TOC(总有机碳)、TN和TP(总磷)对真菌群落结构影响显著。综合分析可知,针阔混交林土壤细菌群落较丰富,杉木林土壤真菌生长较旺盛,而灌丛土壤细菌和真菌多样性均较高,应采取有效措施提高主要林分土壤养分,从而激发微生物的生长,改善土壤环境。     

黄土高原不同植被群落多样性与土壤有机碳密度关系研究

为了探明黄土高原不同植被群落多样性与土壤有机碳密度关系,在黄土高原纸坊沟小流域选取几种典型的植被群落(刺槐Robinia pseudoacacia、铁杆蒿Artemisia gmelinii、长芒草Stipa bungeana、狼牙刺Sophora davidii和柠条Caragana korshinskii),野外调查了植被多样性,同时室内测定0—100 cm土壤有机碳含量、密度以及相关土壤环境因子。结果显示:(1)不同植被群落多样性指数(Margalef丰富度、Mclntosh均匀度和Shannon-Wiener多样性)表现出相同的变化特征。柠条和刺槐群落差异不显著(p>0.05),二者显著低于其他植物群落(p<0.05),长芒草和狼牙刺群落最高,二者之间差异不显著(p>0.05),显著高于其他植物群落(p<0.05); 不同植物群落Simpson优势度指数呈现出相反的变化趋势,刺槐>柠条>铁杆蒿>狼牙刺>长芒草。(2)不同植物群落土壤全氮含量、微生物量碳、微生物量氮、速效养分(硝态氮、铵态氮、速效磷)大致表现为相同的变化特征,均表现为长芒草和狼牙刺群落高于其他群落,而柠条和刺槐群落土壤养分含量低于其他植物群落,不同植物群落土壤pH值表现出相反的变化特征。(3)在垂直方向,土壤有机碳密度随土层深度的增加而逐渐减小,80—100 cm土层土壤有机碳密度最低(p<0.05),0—20 cm土层土壤有机碳密度最高,表现出明显的“表聚性”,相同土层土壤有机碳密度大致表现为长芒草和狼牙刺群落高于其他植物群落。(4)相关性分析显示,不同植物群落多样性指数与有机碳和有机碳密度呈显著或极显著的正相关。冗余分析显示,土壤pH值和微生物量碳含量是主导植被多样性和土壤有机碳密度重要驱动因子; 双因素分析表明植被类型和土层深度对土壤有机碳含量和密度具有显著的影响(p<0.05)。     

阿克苏河中游荒漠河岸林物种多样性与群落稳定性

[目的]探究阿克苏河中游荒漠河岸林群落的物种多样性、稳定性及其之间的关系,在物种水平上对当前群落植物组成的复杂性及部分环境因子的关系进行探讨,为荒漠河岸生态系统的恢复提供理论依据。[方法]在样方调查的基础上,运用格局分析法,物种多样性指数与均匀度指数,聚类分析以及M.Godron稳定性测度对植物群落进行综合评估。[结果]阿克苏河中游荒漠河岸林主要的植物种群均呈现聚集分布的格局,群落结构简单,具有单寡种优势群落的特征。不同地段的植物群落物种分布较为均匀,但丰富度与多样性的高低略有起伏;荒漠河岸林植物群落根据环境因子的聚类结果可分为6个群丛,其中有3个群丛的物种多样性较低。物种组成与土壤水分的关联较为密切;当前植物群落尚未达到稳定阶段,物种多样性与群落稳定性者之间并无任何直接联系。[结论]单一以河水漫灌的方式促进该地区植物多样性的恢复具有一定的困难,考虑以胡杨、柽柳为主要格局,种植深根系的乔灌木以营造局部地区小气候,间接优化生境的方式对荒漠河岸林的植被恢复更为有利。     

Soil carbon content drives the biogeographical distribution of fungal communities in the black soil zone of northeast China

Black soils (Mollisols) are one of the most important soil resources for maintaining food security in China, and they are mainly distributed in northeast China. A previous comprehensive study revealed the biogeographical distribution patterns of bacterial communities in the black soil zone. In this study, we used the same soil samples and analyzed the 454 pyrosequencing data for the nuclear ribosomal internal transcribed spacer (ITS) region to examine the fungal communities in these black soils. A total of 220,812 fungal ITS sequences were obtained from 26 soil samples that were collected across the black soil zone. These sequences were classified into at least 5 phyla, 20 classes, greater than 70 orders and over 350 genera, suggesting a high fungal diversity across the black soils. The diversity of fungal communities and distribution of several abundant fungal taxa were significantly related to the soil carbon content. Non-metric multidimensional scaling and canonical correspondence analysis plots indicated that the fungal community composition was most strongly affected by the soil carbon content followed by soil pH. This finding differs from the bacterial community results, which indicated that soil pH was the most important edaphic factor in determining the bacterial community composition of these black soils. A variance partitioning analysis indicated that the geographic distance contributed 20% of the fungal community variation and soil environmental factors that were characterized explained approximately 35%. A pairwise analysis revealed that the diversity of the fungal community was relatively higher at lower latitudes, which is similar to the findings for the bacterial communities in the same region and suggests that a latitudinal gradient of microbial community diversity might occur in the black soil zone. By incorporating our previous findings on the bacterial communities, we can conclude that contemporary factors of soil characteristics are more important than historical factor of geographic distance in shaping the microbial community in the black soil zone of northeast China.     

河滨缓冲带植物根系和根际微生物特征及其对农业面源污染物去除效果

对河滨缓冲带常见的3种水生植物根系形态特点、活力特征及其土壤微生物群落多样性进行了研究,并对其农业面源污染物的去除效果进行了调查。结果表明,3种水生植物根系形态和活力特征具有显著差异。根系活力表现为水生鸢尾菖蒲千屈菜,与根尖数呈显著相关。同时,3种水生植物具有显著的根际效益,根际土壤微生物生物量显著高于非根际土壤;根际土壤微生物群落数量为细菌放线菌真菌;土壤微生物群落多样性指数为水生鸢尾菖蒲千屈菜,这与3种水生植物根系活力特征表现一致。3种水生植物河滨缓冲区对农业面源污染物TN、TP和CODCr的去除效果表现为水生鸢尾菖蒲千屈菜。说明不同水生植物根系结构导致根系活力不同,由此引起的土壤微生物群落多样性差异对水生植物农业面源污染物去除效果有一定影响。     

The importance of soil characteristics to the structure of alkane-degrading bacterial communities on sub-Antarctic Macquarie Island

An investigation of the influence of soil properties on microbial community dynamics in soil on sub-Antarctic Macquarie Island found that both carbon and nitrogen were important factors in determining soil microbial community structure. The phylogenetic diversity of soil microbial communities in hydrocarbon contaminated and non-contaminated sites was compared to the diversity of hydrocarbon-degrading genes and soil physicochemical characteristics. Genes involved in hydrocarbon degradation including alkane mono-oxygenase, catchecol-2,3-dioxygenase and naphthalene dioxygenase were found throughout the study sites. Terminal restriction fragment length polymorphism analysis of the 16S rRNA and alkB genes found that the patterns of diversity of these two genes were only correlated with each other where measurable levels of hydrocarbons were detected. We found that different sections of the microbial community are affected by different environmental factors depending on whether hydrocarbons were present. The overall microbial community structure as measured by the 16S rRNA gene was most influenced by the presence of carbon both as total organic carbon and as petroleum hydrocarbons. The alkane-degrading community was also influenced by carbon. Where hydrocarbons were present petroleum hydrocarbon concentration as well as the form and concentration of nitrogen present also influenced the alkane-degrading community. This level of complexity in the microbial community dynamics suggests that it is unlikely that one single environmental factor is responsible for structuring microbial communities.     

喀斯特生态系统中乔木和灌木林根际土壤微生物生物量及其多样性的比较

我国喀斯特区域面积分布较广,而喀斯特生态系统的退化已成为当前西南地区面临的严重的生态问题。本研究选取贵州中部两种不同植被类型的生态系统—乔木林和灌木林,以乔木林中的白栎、园果化香和灌木林中的火棘、竹叶椒等主要优势树种为对象,研究不同的植物树种对根际土壤微生物生物量及其细菌群落结构的影响。结果显示:乔木林系统中根际土壤微生物生物量碳、氮显著性高于灌木林,植物的根际效应在乔木林中表现更为显著;同时乔木林中的优势树种通过根系分泌物的作用显著提高根际土壤细菌多样性指数,而灌木林中优势树种的根际土壤微生物量及多样性均未表现出明显的根际效应。因此,植被的演替通过改变土壤微生物的特性影响植物-微生物-土壤之间的物质和能量循环,进一步影响喀斯特生态系统的稳定和健康功能。     

Multiple effects of reindeer grazing on the soil processes in nutrient-poor northern boreal forests

Reindeer grazing has a great influence on the ground vegetation of nutrient-poor northern boreal forests dominated by Cladonia lichens in Fennoscandia. Grazing may influence the soil processes in these systems either by influencing the quality of plant litter, or by indirect effects through the soil microclimate. In order to investigate the mechanisms underlying the effects of reindeer on boreal forest soils, we analyzed litter decomposition, soil and microbial C and N, microbial community composition, and soil organic matter quality in three forest sites with old reindeer exclosures adjacent to grazed areas. There was no effect of grazing on soil C/N ratio, inorganic N concentrations, microbial biomass C, microbial community structure analyzed by phospholipid fatty acid (PLFA) analysis, and organic matter quality analyzed by sequential fractionation, in the soil organic layer. However, microbial N was enhanced by grazing at some of the sampling dates and was negatively correlated with soil moisture, which indicates that increased microbial N could be a stress response to drought. The effect of grazing on litter decomposition varied among the decomposition stages: during the first 1.5 months, the litter C loss was significantly higher in the grazed than the ungrazed areas, but the difference rapidly levelled out and, after one year, the accumulated litter C loss was higher in the ungrazed than the grazed areas. Litter N loss was, however, higher in the grazed areas. Our study demonstrates that herbivores may influence soil processes through several mechanisms at the same time, and to a varying extent in the different stages of decomposition.     

Spatial analysis reveals differences in soil microbial community interactions between adjacent coniferous forest and clearcut ecosystems

Knowledge of how forest management influences soil microbial community interactions is necessary for complete understanding of forest ecology. In this study, soil microbial communities, vegetation characteristics and soil physical and chemical properties were examined across a rectangular 4.57 × 36.58 m sample grid spanning adjacent coniferous forest and clearcut areas. Based on analysis of soil extracted phospholipid fatty acids, total microbial biomass, fungi and Gram-negative bacteria were found to be significantly reduced in soil of the clearcut area relative to the forest. Concurrent with changes in microbial communities, soil macroaggregate stability was reduced in the clearcut area, while no significant differences in soil pH and organic matter content were found. Variography indicated that the range at which spatial autocorrelation between samples was evident (patch size) was greater for all microbial groups analyzed in the clearcut area. Overall, less spatial structure could be resolved in the forest. Variance decomposition using principal coordinates of neighbor matrices spatial variables indicated that soil aggregate stability and vegetation characteristics accounted for significant microbial community spatial variation in analyses that included the entire plot. When clearcut and forest areas were analyzed separately, different environmental variables (pH in the forest area and soil organic matter in the clearcut) were found to account for variation in soil microbial communities, but little of this variation could be ascribed to spatial interactions. Most microbial variation explained by different components of microbial communities occurred at spatial scales other than those analyzed. Fungi accounted for over 50% of the variation in bacteria of the forest area but less than 11% in the clearcut. Conversely, AMF accounted for significant variation in clearcut area, but not forest, bacteria. These results indicate broadly disparate controls on soil microbial community composition in the two systems. We present multiple lines of evidence pointing toward shifts in fungi functional groups as a salient mechanism responsible for qualitative, quantitative and spatial distribution differences in soil microbial communities.     

岷江源区植物群落物种多样性分析

取样调查了岷江源区植被及其环境因子,利用多元统计方法揭示了该区植物群落物种多样性及其与环境因子的关系。结果表明:不同植被类型的物种多样性表现为森林群系 > 灌丛群系 > 草甸群系。柳灌丛、窄叶鲜卑花灌丛、绣线菊灌丛、锦鸡儿灌丛的草本层多样性较高,沙棘灌丛和小果小檗灌丛较低。草甸群系中,白茅草甸和苔草草甸多样性较高,蒿草草甸和高山草甸的物种多样性较低。锦鸡儿灌丛灌木层多样性较高,沙棘灌丛的多样性最低。岷江源区植物群落物种组成受海拔梯度的影响强烈,不同海拔梯度的物种组成差异明显。植物群落在2 900～3 100,3 500～3 700 m海拔段有较高的物种多样性,2 700～2 900,3 100～3 400,3 700～3 900 m海拔段的多样性较低。土壤因子与地形因子之间,海拔和坡向对土壤养分因子的影响较大,主要影响全N、土壤含水量和pH值。地形因子和植被因子之间,海拔和坡向影响灌木层多样性、盖度以及草本层的盖度。植被因子和土壤因子之间,土壤全N量、速效K和土壤含水量影响草本层、灌木层的盖度和多样性。     

海南省文昌市废弃钛矿区物种多样性特征与环境因子的关系

[目的]研究钛矿开采对周边植被物种多样性和分布格局的影响,为废弃钛矿区生物多样性保护和植被恢复提供基础数据。[方法]以海南省文昌市废弃钛矿区为例,利用方差分析、冗余分析(RDA)研究钛矿区土壤因子与乔木、灌木和草本物种多样性差异及分布格局影响。[结果]文昌市废弃钛矿区辐射区域仅有植物28科37属45种,其中乔木类13种,灌木11种,草本21种。钛矿开采不仅对矿区植被产生破坏,同时对辐射区域200 m范围内的植被群落结构也有明显影响;废弃钛矿区物种α多样性指数除Pielou均匀度指数外都以草本最高,显著高于乔木和灌木。随着辐射区域距离的增加,物种多样性指数逐渐递减;通过土壤环境因子与物种多样性RDA排序得出,土壤pH值、土壤含水率、有效磷、有机质和铵态氮是影响物种多样性差异的主要环境因子,它们分别解释了废弃钛矿辐射区物种多样性42.6%,12.4%,8.5%,8.2%和7.9%的差异。[结论]钛矿周边植物物种多样性和分布格局受土壤pH值、土壤含水率等多个环境因子共同影响。矿区周边物种多样性是否受土壤重金属的影响,尚待进一步研究。     

The match between microbial community structure and soil properties is modulated by land use types and sample origin within an integrated agroecosystem

It is of global concern to adopt measures to mitigate land degradation caused by agricultural production systems. One of the strategies proposed is to replace degraded pastures with agrosilvopastoral systems which integrate three different land-use types: crop production, livestock pasture and forestry plantation (denoted iCLF). However, little is known about the differences between iCLF and other land use types in terms of soil microbial community structure. Distance matrices based on individual soil chemical properties and individual soil microbial variables were correlated by Procrustes analysis and these relationships yielded vectors of residuals depicting these correlations (matches). These vectors were used as univariate response variables in an ANOVA framework in order to investigate how the match sizes (the strength of correlation/covariance) between individual soil chemical properties and individual soil microbial variables vary across land use types (levels: iCLF; degradated pasture; improved pasture; and a native cerrado fragment) and also across sample origin within iCLF (levels: soil samples under more influence of the exotic tree forest stand; soil samples under influence of the pasture; samples within the transition between the forest stand and the pasture). We were able to obtain insights into the fact that the land use distinction can be driven by more than just individual soil chemical and microbial variables. The integration of crop, livestock and forestry promoted a dominance of fungi in this low fertility and low pH environment. P availability and the composite variable exchangeable base cations (Ca⁺², Mg⁺², K⁺) were the soil properties whose strengths of correlation (match sizes) with individual microbial variables were the most affected by land use type and sampling origin within iCLF. While the strength of the correlation between soil microbial structure variables and P availability was typically land use type dependent, the response of the microbial structure to exchangeable base cations was mainly affected by the sample origin within iCLF. Finally our results point towards the conclusion that increases in the heterogeneity of vegetation within integrated crop, pasture and forestry systems are an important driver of microbial community response to environmental changes, and may be one means by which to increase the sustainability of tropical agroecosystems.     

黄土高原油松根际土壤酶活性及真菌群落多样性研究——以黄龙山林场为例

为了解黄土高原油松林根际土壤酶活性和真菌群落多样性,本研究分析了陕西黄龙县不同样区油松根际土壤脲酶、碱性磷酸酶、多酚氧化酶和过氧化氢酶活性,并采用巢式PCR-变性梯度凝胶电泳(PCR-DGGE)技术研究了油松根际土壤中真菌群落多样性。结果表明,该地区油松根际真菌群落相似性较高,但受坡向、海拔、土壤水分及人类扰动等诸多因素的影响,不同样区的真菌群落多样性和土壤酶活性存在差异。油松根际各土壤酶活性均表现出坡顶样地高于坡底样地,阴面样地高于阳面样地,林区路旁样地由于采样环境不同于林中样地,酶活性介于其他样地之间;丰富度(S)、Shannon-Wiener指数(H)、Simpson指数(D)、均匀度指数(EH)分析表明,该区域油松根际土壤真菌群落多样性分布特征与酶活性分布特征相一致。相关性分析表明,除过氧化氢酶外,其余酶活性之间、以及与真菌多样性均呈显著正相关(p0.05);土壤含水量与真菌多样性和土壤酶活性除多酚氧化酶外均呈显著正相关(p0.05);而土壤p H与各种酶活性之间均未达显著相关水平(p0.05)。土壤含水量是影响该地区真菌群落多样性与土壤酶活性主要因素之一。     

退化喀斯特植被恢复与土壤微生物特征的关系

The mechanism of vegetation restoration on degraded karst regions has been a research focus of soil science and ecology for the last decade.In an attempt to preferably interpret the soil microbiological characteristic variation associated with vegetation restoration and further to explore the role of soil microbiology in vegetation restoration mechanism of degraded karst regions,we measured microbial biomass C and basal respiration in soils during vegetation restoration in Zhenfeng County of southwestern Guizhou Province,China.The community level physiological profiles(CLPP) of the soil microbial community to were estimated determine if vegetation changes were accompanied by changes in functioning of soil microbial communities.The results showed that soil microbial biomass C and microbial quotient(microbial biomass C/organic C) tended to increase with vegetation restoration,being in the order arboreal community stage > shrubby community stage > herbaceous community stage > bare land stage.Similar trend was found in the change of basal respiration(BR).The metabolic quotient(the ratio of basal respiration to microbial biomass,qCO 2) decreased with vegetation restoration,and remained at a constantly low level in the arboreal community stage.Analyses of the CLPP data indicated that vegetation restoration tended to result in higher average well color development,substrate richness,and functional diversity.Average utilization of specific substrate guilds was highest in the arboreal community stage.Principle component analysis of the CLPP data further indicated that the arboreal community stage was distinctly different from the other three stages.In conclusion,vegetation restoration improved soil microbial biomass C,respiration,and utilization of carbon sources,and decreased qCO 2,thus creating better soil conditions,which in turn could promote the restoration of vegetation on degraded karst regions.     

土壤微生物多样性与地上植被类型关系的研究进展

土壤微生物是生态系统中重要的组成成分,土壤微生物多样性代表着微生物群落的稳定性,对植物的生长发育和群落结构的演替具有重要作用。同时地上植被也影响土壤微生物多样性,地上植被和地下微生物间具协同作用和正负反馈效应的互作机制。探讨植被和土壤微生物多样性之间的互作关系,为植物保护和生态系统的可持续发展研究提供参考。     

不同年限毛竹-白及复合系统土壤微生物群落多样性特点

为了揭示毛竹-白及复合经营系统中植物-土壤间的互作关系,在安徽省广德市邱村镇选择3个不同套种年限(1、3和4年)的毛竹-白及复合林分为研究对象,采用Biolog-ECO微平板技术,结合主成分分析对培养72 h土壤微生物群落功能多样性及其碳代谢特征进行分析.结果 表明,毛竹-白及复合林分的土壤微生物碳源平均颜色变化率、S...