Dicyandiamidabbau im Boden in Abhängigkeit von der Temperatur

Effect of temperature on the breakdown of dicyandiamide in the soil The breakdown of dicyandiamide in a soil (sandy silty loam, pH 6.2, 0.13 % N) was investigated in relation to temperature. 1. The rate of conversion of dicyandiamide (DCD) (20 mg DCD-N/100 g soil) to guanylurea increased with rising temperature (10°–90°C). After 20 days, 14–100 % of the added DCD was metabolized. Small amounts of DCD (0.67 resp. 1.34 mg DCD-N/100 g soil) were broken down completely within 20–80 days at 8°–20°C. 2. Guanylurea was transformed to guanidine and then to ammonium. Increasing temperature in the region of 10° and 30°C accelerated the transformation. At higher temperatures (up to 70°C) an accumulation of guanidine occurred.     

Einfluß variierter Temperatur und Feuchte auf mikrobielle Aktivitäten im Boden unter Laborbedingungen

Influence of varied soil temperature and moisture on microbial activities under laboratory conditions Under laboratory conditions the influence of temperature (10°C, 20°C, fluctuation from 5° to 30°C within 12 h with additional freezing for 3 days) and soil moisture (30%, 60% w.h.c., remoistening to 60% for 1 week) on several microbial activities was investigated. The biomass-related, glucose-induced short-term respiration and the dehydrogenase activity (TTC reduction) were higher at 10°C in most cases as compared to 20°C. Independent of freezing fluctuating temperature caused the lowest activities. The nitrogen mineralization (including nitrification), however, was affected in the opposite way. No marked influences were observed with β-glucosidase, arylsulfatase, and alkaline phosphatase. In the sandy loam nearly no effects of the soil moisture occurred and in the loamy sand especially the dehydrogenase activity was higher at 30% w.h.c., whereas the nitrogen mineralization was lower. From the results it can be concluded, that ecological conditions favouring mineralization without substrate addition may even reduce microbial biomass by decomposition.     

Der Einfluß von Klärschlamm auf die mikrobielle Tätigkeit im Boden

The influence of sewage sludge on microbial activity in soil The effect of untreated, pasteurized and irradiated sewage sludge on soil biology was studied in model and field experiments. Sewage sludge from the sewage plant Geiselbullach in the west of Munich city was used for these experiments. The investigations were performed with 4 soil types according to the different locations. All the results demonstrate that there is an increase in biomass after application of sewage sludge while there were definite influences from the location. No significant relation could be observed according to sewage sludge treatment. Under favourable lab conditions the mineralization of the organic matter applied to the soil with higher amounts of sewage sludge induces in short time a definite decrease in pH. In accordance with these changes in pH there is a corresponding decrease in microbial biomass. Early results on the influence of microbial N-cycle demonstrated that amounts of sewage sludge applied under practical conditions brought an increase in denitrification capacity of the soil. In the experiment with soils nitrification rate was doubled after sewage sludge application.     

Abbau und biozide Wirkung von Pflanzenschutz-und Schädlingsbekämpfungsmitteln im Boden am Beispiel von Kelevan

Degradation and biocide effect of chemical plant protecting agents and pesticides in soils by the example of the insecticide Kelevan By the example of the insecticide Kelevan it is proved that by means of a combined test plan degradation and biocide effect of chemical plant protecting agents and pesticides in soils can be tested simultaneously. For this test two different test soils as described in leaflet No. 36 of the Biologische Bundesanstalt (BBA), Braunschweig, are each divided in test samples of about 200 g dry matter. To answer the question whether besides the biotic an abiotic degradation of Kelevan and its primary subsequent products takes place in top soil, too, one part of the soil samples was sterilized by overheated steam. Afterwards these and the non-sterilized soil samples were treated with known amounts of Kelevan[cage-U-¹⁴C] and in accordance to leaflet No. 36 of the BBA stored in the dark at 22°U65% r. h. or under field conditions for different periods. To investigate the effect of Kelevan and its metabolites on microorganisms in top soil, further soil samples were treated with increasing amounts of Kelevan and also stored for different periods. At the end of storage periods on an average W,2 % of applicated radioactivities were recovered in the soil samples with Kelevan[cage-U-¹⁴C]. Whereas readioactivities of sterilized soil samples were nearly quantitatively extractable, increasing radioactivity amounts were held back under the same extraction conditions by the native soil samples, which were present as organic residue components of Kelevan(cagc-U-¹⁴C) and not as ¹⁴C-containing carbonate. During degradation, in both test soils as well under laboratory conditions as under field conditions, about one third of Kelevan[cage-U-¹⁴C] was transferred within 30 months via Kelevan acid[cage-U-¹⁴C] to Chlordecon[cage-U-¹⁴C] and about two thirds were transferred into various unknown ¹⁴C-labelled degradation products. The results of microbiological investigation prove that microorganisms were evidently neither selected nor decimated in both test soils by Kelevan and its degradation products.     

Enzymatisch-colorimetrische Bestimmung von Purin-Stickstoff im Boden

Enzymatic and colorimetric determination of purine nitrogen in soils This paper describes a method for determination of purine nitrogen of nucleic acid components in soils. The method is based on the enzymatic degradation of purine containing nucleotides, nucleosides and nucleobases to uric acid which is determined colorimetrically. The nitrogen content of a rendzina, for example, related to the monomeric nucleic acid compounds is very low and was found to be less than 1 percent compared to the total nitrogen content.     

N-Umsatz von verschiedenem Pflanzenmaterial im Boden in Gefäß- und Feldversuchen

Turnover of nitrogen of different plant material in the soil in pot and field trials In pot and field trials, effects of various plant materials like green rape, sugar beet leaves, potato foliage, straw of wheat, maize, or field beans, on the turnover of N in the soil (mineralization, N_min-concentrations, NO₃-leaching) and on yield and N uptake by some crops was tested in relation to mineral N fertilizer application. In both experiments, widely corresponding results were obtained: Irrespective of the N contents of the plant material, biological immobilization of N always was combined with beginning mineralization (pot trial: decrease of NO₃ leaching in late fall). Green rape (C/N = 12/1) showed already after 4 weeks in the field trial a marked net mineralization (N_min) of 20–30% of the added nitrogen which was quantitatively reflected in higher removals of N. Beet leaves (C/N = 20/1) were decomposed at a much slower rate in combination with an increase in N removals of subsequent crops by 6-20 % depending on the rate of mineral fertilizer application. All types of straw (CM = 57-8611) and potato foliage (CM = 73/1) caused a marked biological immobilization of N which resulted in reduced N removals depending on turnover rate (potato foliage: fast, wheat straw: slow) and N contents of the organic material. Different plant residues (straw, leaves) were applicated on a harvested field with cereal to test comparatively one part of the total value of different preceding crops; at onset of vegetation the N_min-contents in soil, depending on the climatic conditions during the non-growing season for mineralization, were analyzed.     

Schwermetalle im Boden eines Klärschlamm-Versuchsfeldes

Heavy metals in soil of a sewage sludge experimental field The total amounts of Zn, Cd, Pb, Cu, Cr and Ni were determined in different depths of soils which have obtained sewage sludges in amounts between 180 and 1620 dt dry matter/ha. The elements Zn, Cd. Pb and Cu have been most enriched in the first twenty cm of the soils. The contents of Zn, Cd and Pb in the depth of 40–60 cm also showed a significant increase. The treshold values for Zn and Cd in soils were almost attained respectivly slightly exceeded in the first twenty cm of the soil which has obtained 1440 dt dry matter sewage sludge per ha.     

Umsatz von 15N markiertem Nitratstickstoff im Boden in Abhängigkeit von Strohdüngung und Bodenfeuchte

Turnover of ¹⁵N labelled nitrate nitrogen in soil as related to straw application and soil moisture In incubation experiments the effect of straw application on the turnover of ¹⁵N labelled nitrate has been studied at two soil moisture levels (brown podzolic soil). High ¹⁵N losses were found at the high soil moisture level. These losses are supposed to originate from denitrification. At both soil moisture levels straw application reduced significantly the ¹⁵N losses. Straw application resulted in a remarkable decrease of the ¹⁵NO₃ content in the soil and promoted the incorporation of ¹⁵N into the organic soil fraction. The incorporation of ¹⁵N into α-amino N and particularly into the rest hydrolyzable-N fraction was favoured by straw, while the incorporation into the amide-N fraction was hampered. Exhaustive cropping (Lolium multiflorum and Sinapis alba) on the soil incubated before with ¹⁵N, showed that the ¹⁵N incorporated into the organic fraction was poorly available. Thus straw application resulted in significant yield depression. It is assumed that the fraction of the rest hydrolyzable N is hardly available to plants.     

Bedeutung der Mikroorganismen für die Bildung von Aggregaten im Boden

Significance of microorganisms in aggregate formation In soils microorganisms are the biological link between the physicochemical processes of synthesis and degradation. Because of their metabolic activities and their mechanical binding capacities they are directly involved in the development of soil structure. This review attempts to summarize knowledge of experimental work concerning aggregate formation by microorganisms. Especially distribution, adhesion processes, significance of biopolymers in aggregate formation and destabilization will be covered. The current state of art and open research areas will be indicated.     

Der Wasserhaushalt von Fichte in Abhängigkeit von Boden und Atmosphäre

The water regime of spruce in relation to soil and atmosphere Simple observations of the water regime of potted spruce trees and measurements in the field are reported, followed by a discussion of spruce water potential in relation to soil and atmosphere water potentials. When the soil is sufficiently moist water regime of spruce is determined by the atmosphere. As higher the atmospheric demand and as lower the hydraulic conductivity of the soil as more difficult it is for a spruce plant to supply transpired water and more and more the potential transpiration rate is reduced. For short term aspects water storage in leaf tissues is of high importance. Observations show the possibility of water uptake by needles if wetted by rain or dew. The maximum amount of this way of water absorption is also given by the internal storage capacity.     

Validierung eines 2-Regionen-Modells zur Simulation des Stofftransportes im Boden anhand von Laborsäulen- und Feldversuchen

Validation of a 2-domain-model for the simulation of solute transfer in soils by laboratory and field experiments The simulation of water and solute transfer in the unsaturated zone on the basis of the classical convective dispersive model gives dissatisfactory results in many cases. Especially in the zone of evapotranspiration the observed penetration depths of surface-applied chemicals often exceed calculated values. This has been attributed to preferential flow in domains with accelerated flow. The aim of the investigations presented here was to extend the conventional theory of solute transfer in order to enable improved model predictions without introducing too much complexity to the model which might reduce its applicability. The mobile-immobile-concept of Coats and Smith (1964) was chosen to extend the model FLOTRA (Wagner et al., 1992), which is based on the convective dispersive approach. The model parameters additionally needed by the model are the immobile water content θ_im and the solute transfer coefficient α. The applicability of the model was tested on the basis of several laboratory and field experiments. It is shown, that with the mobile-immobile-approach modeling results of water and solute transfer in the top soil layers, which are most important in view of soil and groundwater protection, were improved compared to the calculations with the classical convective dispersive model.     

Die potentiometrische Bestimmung von Nitrat im Boden mit einer Membranelektrode

Soil-Nitrate Determination with the NO₃-sensitive Electrode A NO₃-sensitive ion electrode (Orion, No. 92–07) was tested to determine the nitrate concentration in soil extracts. Some analytical problems are to be respected: 2. During a 2–8 week period of measurement the electrode gradually lost its potential (Figure 2). Therefore it is necessary to replace the sensitive elements (ion-exchanger, nitrate concentration and membran of electrode) at a certain level (in this 165 mV with 0.22 ppm NO,-N-solution). 3. Additional problems are from the heterogenity of thes oil(s = 0.10mg NO₃-N/100g soil in this experiment). With 4 replications in sampling at given site or date, significant differences in the range of 20 kg NO,-N/ha have been obtained. Observing the before mentioned precautions the correlation between nitrate content 1. The optimal range for measurements can be calculated by application of the Peters-Nernst equation between 1 to 10 ppm NO₃-N (Figure 1). This equation is influenced by the used electrode. Because this influence changed, it is necessary, to measure the standard solution before and after the series. determined by the nitrate electrode and conventional method (r = 0.92, Figure 3) was highly significant. The deviation of 0.28 ppm has no influence on the agronomy conclusions based on the nitrate determinations.     

Auswirkungen der Gamma-Bestrahlung und Pasteurisierung von Klärschlamm auf seine mikrobiellen Eigenschaften und die Mineralisierung im Boden

Effect of γ-irradiation and pasteurization of sewage sludge to the microbiological properties and the mineralization in soil Hygienic aspects of sewage sludge application in agricultural practice are of increasing importance. Because parasites are extremely sensitive to γ-irradiation a dose of 300 krad is equivalent to pasteurization. The total bacteria count of the sewage sludge is reduced by this dosage by 90 to 99%. Enzymic activity is reduced after radiation at a rate of about 39%. Especially amylase, catalase, and alkaline phosphatase are extremely sensitive to irradiation. Mineralization studies of sewage sludge with different pretreatments in different soil types indicated no significant differences.