共查询到20条相似文献，搜索用时 15 毫秒
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Michael T. Walsh DVM Frank S. Pipers DVM PhD† Caroline A. Brendemuehl DVM‡ Frank L. Murru BS 《Veterinary radiology & ultrasound》1993,34(3):213-219
The use of sonography was evaluated in eleven sharks as a potential diagnostic and investigative tool. Individual sharks were anesthetized with MS-222 (Tricane Methane Sulphonate) and sonograms recorded from various planes using a 3 MHZ linear array scanner. Sonographic images were compared with corresponding postmortem cross sections. Heart rate and rhythm were monitored with real time sonography in an attempt to gauge the depth of the anesthesia. Heart rates ranged from 12–46 bpm. Initial results of this study suggest that sonography may be a useful technique in shark species where the application of radiographic techniques is limited by the lack of adipose tissue and the need to maintain the animal in water. 相似文献
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Ioannis L. Oikonomidis Elspeth Milne Chiara Piccinelli 《Journal of veterinary diagnostic investigation》2021,33(4):670
We evaluated the performance of the Advia 2120 (Siemens) differential leukocyte count (A-Diff) compared to the manual method (M-Diff) in rabbits. EDTA-anticoagulated blood samples collected for diagnostic purposes were analyzed within 6 h of collection. The M-Diff was performed blindly by 2 observers on blood smears by counting 200 cells. We initially included 117 samples; 25 samples were excluded because of suboptimal gating of leukocytes in the Advia peroxidase cytogram or poor blood smear quality. The correlation between the A-Diff and M-Diff was very high for heterophils (r = 0.924, p < 0.001) and lymphocytes (r = 0.903, p < 0.001), high for basophils (r = 0.823, p < 0.001), moderate for monocytes (r = 0.645, p < 0.001), and low for eosinophils (r = 0.336, p = 0.001). The Passing–Bablok regression analyses revealed a small-to-moderate constant error for lymphocytes and a slight constant error for basophils. Small proportional errors were detected for heterophils, lymphocytes, and eosinophils. The Bland–Altman analyses revealed that the Advia significantly underestimates heterophils and overestimates lymphocytes compared to M-Diff. The biases for the other leukocytes were minimal and likely clinical insignificant; however, our results, particularly for eosinophils, should be interpreted cautiously given the observed low percentages in our samples. Given the observed biases in heterophil and lymphocyte percentages in the Advia 2120 CBC results in rabbits, method-specific reference intervals should be used. The Advia can recognize leporine basophils. Evaluation of blood smears is still recommended to investigate abnormal results and erroneous cytograms reported by the Advia. 相似文献
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Ioannis L. Oikonomidis;Christos Brozos;Theodora K. Tsouloufi;Evangelos Kiossis;Maria Kritsepi-Konstantinou; 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2024,53(1):57-62
Although widely used, the ADVIA 120 hematology analyzer has not been previously validated for determining the differential leukocyte count in goats. 相似文献
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J.L. Sottnik S. Rao M.H. Lafferty D.H. Thamm P.S. Morley S.J. Withrow S.W. Dow 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2010,24(6):1439-1444
Background: Identification of biomarkers that predict outcomes in dogs with osteosarcoma (OSA) would be valuable to veterinarians and owners. Leukocyte numbers in peripheral blood are associated with outcomes in some types of cancer in humans. Hypothesis/Objectives: We hypothesized that increased numbers of monocytes would be associated with reduced disease‐free interval (DFI) in dogs with OSA. Animals: Medical data from 69 dogs with appendicular OSA treated with amputation and chemotherapy were selected for study. Methods: Retrospective study. Statistical associations were assessed by univariate and multivariate analysis. Information about DFI and leukogram values, tumor location, and serum alkaline phosphatase was abstracted from the medical record. Results: Higher numbers of circulating monocytes (>0.4 × 103 cells/μL) and lymphocytes (>1.0 × 103 cells/μL) before treatment were found to be significantly (P < .05) associated with shorter DFI in dogs with OSA. Other parameters associated with poor outcomes were increased alkaline phosphatase, primary tumor location, and age. Conclusion and Clinical Importance: These results indicated that pretreatment evaluation of monocyte and lymphocyte counts provided prognostic information for dogs with appendicular OSA. Notably, most animals in this study had monocyte counts within the normal reference range, indicating that variations within the reference range of leukocyte values might also have prognostic significance. 相似文献
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Harms C Ross T Segars A 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2002,31(3):111-115
Background — Elasmobranchs (sharks, skates, and rays) are of commercial, sport, research, and exhibit importance, however, blood chemistry reference values have been determined for few of these species. Objectives — The purpose of this study was to establish plasma biochemistry and PCV reference values for wild bonnethead sharks (Sphyrna tiburo). Methods — Heparinized blood samples were collected from 24 bonnethead sharks at the time of capture in trawl nets off the coast of South Carolina and Georgia. Weight, length, PCV, total solids (TS, by refractometry), and plasma biochemical analyses were done using standard techniques. Wilcoxon rank‐sum and Kendall tau b tests were used to compare values by animal size, boat and sex; 1–way ANOVA was used to compare TS and total protein (TP) concentrations. Results — Median (quartiles; minimum‐maximum) values were as follows: PCV 22% (22%, 26%; 17–28%), TS 6.3 (6.0, 6.8; 5.8–7.5) g/dL, total protein 2.9 (2.7,3.4; 2.2–4.3) g/dL, albumin 0.4 (0.4,0.4; 0.3–0.5) g/dL, globulins 2.6 (2.3,3.0; 1.9–3.8) g/dL, sodium 282 (279, 285; 273–292) mmol/L, potassium 7.3 (6.4, 7.9; 5.7–9.2) mmol/L, chloride 290 (285, 296; 277–304) mmol/L, total CO2 3 (2, 4; 0–5) mmol/L, calcium 16.8 (16.2,17.4; 15.8–18.2) mg/dL, phosphorus 8.8 (7.5,10.0; 5.9–12.7) mg/dL, urea nitrogen 1004 (986, 1028; 944–1068) mg/dL, creatinine <0.1 mg/dL, glucose 184 (165, 191; 155–218) mg/dL, aspartate aminotransferase 42 (33, 66; 15–132) U/L, lactate dehydrogenase <5 U/L, creatine kinase 82 (47, 233; 18–725) U/L, and osmolality 1094 (1078,1111; 1056–1139) mOsm/kg. No differences based on sex were detected. TS and total TP values were related by the fitted line TS = (1.006 × TP) + 3.318. Conclusions — Values reported here will be useful for evaluating the health status of bonnetheads in wild and captive research conditions and in exhibits. 相似文献
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Furlanello T Tasca S Caldin M Carli E Patron C Tranquillo M Lubas G Solano-Gallego L 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):42-46
BACKGROUND: Artifactual changes in blood may occur as a consequence of delayed analysis and may complicate interpretation of CBC data. OBJECTIVE: The aim of this study was to characterize artifactual changes in canine blood, due to storage, using the ADVIA 120 hematology analyzer. METHODS: Blood samples were collected into EDTA from 5 clinically healthy dogs. Within 1 hour after blood sample collection and at 12, 24, 36 and 48 hours after storage of the samples at either 4 degrees C or room temperature (approximately 24 degrees C), a CBC was done using the ADVIA 120 and multispecies software. A linear mixed model was used to statistically evaluate significant differences in values over time, compared with initial values. RESULTS: The HCT and MCV were increased significantly after 12 hours of collection at both 4 degrees C and 24 degrees C, and continued to increase through 48 hours. The MCHC initially decreased significantly at 12-24 hours and then continued to decrease through 48 hours at both temperatures. Changes in HCT, MCV, and MCHC were greater at 24 degrees C than at 4 degrees C at all time points. A significant increase in MPV and a decrease in mean platelet component concentration were observed at all time points at 24 degrees C. Samples stored at 24 degrees C for 48 hours had significantly higher percentages of normocytic-hypochromic RBCs, and macrocytic-normochromic RBCs, and lower platelet and total WBC counts. CONCLUSIONS: Delayed analysis of canine blood samples produces artifactual changes in CBC results, mainly in RBC morphology and platelet parameters, that are readily detected using the ADVIA 120. Refrigeration of specimens, even after 24 hours of storage at room temperature, is recommended to improve the accuracy of CBC results for canine blood samples. 相似文献
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Caitlin N Cadaret Marytza D Abebe Taylor L Barnes Robert J Posont Dustin T Yates 《Journal of animal science》2021,99(6)
Pathogenic infections increase morbidity and reduce performance in livestock, and thus understanding the comprehensive physiological changes associated with infections can benefit production sustainability. In this study, we sought to investigate such physiological responses to an acute immune challenge in lambs. Polypay wethers received single IV injections of 1.5 µg/kg lipopolysaccharide endotoxin (LPS-injected; n = 6) or saline (controls; n = 6). Corneal temperatures (via infrared thermography), rectal temperatures, blood, plasma, and saliva were assessed every 2 hr for 10 hr after injections. Blood was also assessed at 24 hr. LPS-injected lambs exhibited elevated (P < 0.05) corneal and rectal temperatures that peaked at 4 hr but were still slightly greater (P < 0.05) than controls at 10 hr. Circulating total white blood cells, monocytes, and granulocytes were reduced (P < 0.05) in LPS-injected lambs within the first 4 hr but were subsequently greater (P < 0.05) than in controls. Lymphocytes were reduced (P < 0.05) in LPS-injected lambs over the first 8 hr and did not differ from controls thereafter. Red blood cells, hematocrit, and hemoglobin were increased (P < 0.05) in LPS-injected lambs over the first 6 hr, indicating mild dehydration. Blood glucose briefly increased (P < 0.05) in LPS-injected lambs at 2 hr but was less (P < 0.05) than in controls thereafter. Blood lactate was greater (P < 0.05) in LPS-injected lambs between 6 and 10 hr after injections, which together with reduced (P < 0.05) CO2 partial pressure indicated a metabolic shift toward glycolysis. LPS-injected lambs exhibited a transient increase (P < 0.05) in plasma TNFα at 2 and 4 hr only and sustained increases (P < 0.05) in CXCL9 and CXCL10 beginning at 6 and 4 hr, respectively. They also exhibited a mild, paradoxical increase (P < 0.05) in the anti-inflammatory sFRP3. Salivary TNFα was increased (P < 0.05) in LPS-injected lambs at 2 hr only. Regression analyses indicated that rectal temperatures were a generally poor predictor of the other inflammatory components in this study, with the exception of circulating leukocyte populations. Likewise, correlations among the 10 cytokines measured in this study were generally weak, with notable exceptions between CXCL9 and CXCL10 and between IL-21 and IFNγ. These findings demonstrate that physiological changes to even short-lived immune challenges are dynamic in nature and persist beyond the time frame of febrile responses and other common assessments. 相似文献
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Moritz A Fickenscher Y Meyer K Failing K Weiss DJ 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(1):32-38
Background: The ADVIA 120 is a laser-based hematology analyzer with software applications for animal species. Accurate reference values would be useful for the assessment of new hematologic parameters and for interlaboratory comparisons.
Objective: The goal of this study was to establish reference intervals for CBC results and new parameters for RBC morphology, reticulocytes, and platelets in healthy dogs and cats using the ADVIA 120 hematology system.
Methods: The ADVIA 120, with multispecies software (version 1.107-MS), was used to analyze whole blood samples from clinically healthy dogs (n=46) and cats (n=61). Data distribution was determined and reference intervals were calculated as 2.5 to 97.5 percentiles and 25 to 75 percentiles.
Results: Most data showed Gaussian or log-normal distribution. The numbers of RBCs falling outside the normocyticnormochromic range were slightly higher in cats than in dogs. Both dogs and cats had reticulocytes with low, medium, and high absorbance. Mean numbers of large platelets and platelet clumps were higher in cats compared with dogs.
Conclusions: Reference intervals obtained on the ADVIA 120 provide valuable baseline information for assessing new hematologic parameters and for interlaboratory comparisons. Differences compared with previously published reference values can be attributed largely to differences in methodology. 相似文献
Objective:
Objective: The goal of this study was to establish reference intervals for CBC results and new parameters for RBC morphology, reticulocytes, and platelets in healthy dogs and cats using the ADVIA 120 hematology system.
Methods:
Methods: The ADVIA 120, with multispecies software (version 1.107-MS), was used to analyze whole blood samples from clinically healthy dogs (n=46) and cats (n=61). Data distribution was determined and reference intervals were calculated as 2.5 to 97.5 percentiles and 25 to 75 percentiles.
Results:
Results: Most data showed Gaussian or log-normal distribution. The numbers of RBCs falling outside the normocyticnormochromic range were slightly higher in cats than in dogs. Both dogs and cats had reticulocytes with low, medium, and high absorbance. Mean numbers of large platelets and platelet clumps were higher in cats compared with dogs.
Conclusions:
Conclusions: Reference intervals obtained on the ADVIA 120 provide valuable baseline information for assessing new hematologic parameters and for interlaboratory comparisons. Differences compared with previously published reference values can be attributed largely to differences in methodology. 相似文献
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Tatiana C. Weisbrod Ramiro Isaza Carolyn Cray Laurie Adler Nicole I. Stacy 《The Veterinary quarterly》2021,41(1):30
Unique features of elephant hematology are known challenges in analytical methodology like two types of monocytes typical for members of the Order Afrotheria and platelet counts of the comparatively small elephant platelet. To investigate WBC differential and platelet data generated by an impedance-based hematology analyzer without availability of validated species-specific software for recognition of elephant WBCs and platelets, compared to manual blood film review. Blood samples preserved in ethylenediaminetetraacetic acid (EDTA) of 50 elephants (n = 35 Elephas maximus and n = 15 Loxodonta africana) were used. A Mann-Whitney test for independent samples was used to compare parameters between methods and agreement was tested using Bland-Altman bias plots. All hematological variables, including absolute numbers of heterophils, lymphocytes, monocytes, eosinophils, basophils, and platelets, were significantly different (p < 0.0001) between both methods of analysis, and there was no agreement using Bland-Altman bias plots. Manual review consistently produced higher heterophil and monocyte counts as well as platelet estimates, while the automated analyzer produced higher lymphocyte, eosinophil, and basophil counts. The hematology analyzer did not properly differentiate elephant lymphocytes and monocytes, and did not accurately count elephant platelets. These findings emphasize the importance of manual blood film review as part of elephant complete blood counts in both clinical and research settings and as a basis for the development of hematological reference intervals. 相似文献
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Nicholas Otway 《Veterinary Medicine and Science》2020,6(3):579-586
The paired lateral abdominal veins (LAV) provide alternative venipuncture sites in grey nurse sharks (Carcharias taurus, Rafinesque 1810) and their efficacy was assessed using morphometrics, necropsies, ultrasound‐guided blood sampling and by comparing serum biochemistry between the LAV and caudal vein (CV) with values from the latter published previously. The mean length of the CV was 2.8% of total length (TL) whereas each LAV was 22.4% of TL and, when combined, was approximately 16 times longer than the CV. The mean tissue thickness overlying each LAV increased significantly (p < .001) with increasing TL and ranged from 3.5 to 33.8 mm in the smallest to largest shark. The mean internal diameter of the paired LAV also increased significantly (p < .001) with increasing TL and was equal to or exceeded the CV. Experienced SCUBA divers captured 56 free‐living grey nurse sharks and 46 healthy animals were sampled for blood from the LAV and CV with minimal risk to the animals or staff. Venipuncture of the LAV (n = 16) was easily accomplished using ultrasound guidance with a 38 mm/18‐gauge needle, whereas standard methods were used with the CV (n = 30). Serum biochemistry was compared (t‐tests) and none of the biochemical analytes differed significantly between the LAV and CV. The paired LAV produced representative blood samples and could also be used for fluid therapy and/or intravenous anaesthesia as has been done in other sharks. It is recommended that veterinary/husbandry staff familiarize themselves with the paired LAV and consider their use in the future. 相似文献
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Harold Tvedten Päivi Johansson 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2010,39(2):190-192
Background: The large size of many feline platelets and the high frequency of platelet aggregation often results in falsely low platelet counts in this species. A combination of optical platelet counting to detect even large platelets and the use of prostaglandin E1 (PGE1) to inhibit platelet clumping may increase the accuracy of feline platelet counting. Objective: The objective of this study was to compare platelet counts in feline whole blood samples with and without the addition of PGE1 and using different analytical methods in a clinical setting. Methods: Platelet counts were determined in 10 feline patients in a referral veterinary hospital using 2 sample types (EDTA, EDTA with PGE1) and 2 methods of analysis (optical counting [PLT‐O] and impedance counting [PLT‐I]) on the Sysmex XT 2000 iV analyzer. Results: All PGE1–PLT‐O samples had platelet counts of >200 × 109/L. Mean platelet count using PGE1–PLT‐O (410,256±178 × 109/L) was significantly higher (P<.03) compared with PGE1–PLT‐I (256±113 × 109/L), EDTA–PLT‐O (238±107 × 109/L), and EDTA–PLT‐I (142±84 × 109/L) methods. Depending on the method, platelet counts in 2 to 7 of 10 cats were <200 × 109/L when PGE1‐PLT‐O was not used. A slightly increased platelet count in response to treatment of a feline patient with thrombocytopenia would have been missed without use of PGE1–PLT‐O. Conclusions: Using PLT‐O analysis on EDTA samples containing PGE1 provides higher, and therefore likely more accurate, feline platelet counts in a clinical setting. 相似文献
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Lilliehöök I Wall H Tauson R Tvedten H 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(3):133-138
Background: Automated hematology instruments commonly are used for mammalian blood analysis, but there is a lack of accurate automated methods available for avian leukocyte analysis. Objective: The aim of this study was to validate differential leukocyte counts in blood from chickens using the Cell-Dyn 3500 hematology system and avian-specific software.
Methods: Blood samples were collected in lithium-heparin tubes from 2 groups (n = 84 and n = 139) of laying hens. Manual 200-cell differential counts were done on routinely-stained blood smears, and manual total granulocyte counts (heterophils and eosinophils) were done using an eosinophil stain in a counting chamber. Automated differential counts were done using VET 2.3, a research and development version of avian-specific software for the Cell-Dyn 3500. Results were analyzed using Pearson's correlation and difference plots.
Results: Automated granulocyte counts from the Cell-Dyn were in good agreement with manual granulocyte counts ( r = 0.93 and 0.80 for the 2 study groups). No correlation was found between automated and manual lymphocyte counts. Correlation coefficients for monocyte counts were 0.70 and 0.43. Conclusion: Automated leukocyte results from the Cell-Dyn using VET 2.3 software were not fully accurate. Total granulocyte counts may be of clinical usefulness, but results obtained for other parameters were unreliable. 相似文献
Methods: Blood samples were collected in lithium-heparin tubes from 2 groups (n = 84 and n = 139) of laying hens. Manual 200-cell differential counts were done on routinely-stained blood smears, and manual total granulocyte counts (heterophils and eosinophils) were done using an eosinophil stain in a counting chamber. Automated differential counts were done using VET 2.3, a research and development version of avian-specific software for the Cell-Dyn 3500. Results were analyzed using Pearson's correlation and difference plots.
Results: Automated granulocyte counts from the Cell-Dyn were in good agreement with manual granulocyte counts ( r = 0.93 and 0.80 for the 2 study groups). No correlation was found between automated and manual lymphocyte counts. Correlation coefficients for monocyte counts were 0.70 and 0.43. Conclusion: Automated leukocyte results from the Cell-Dyn using VET 2.3 software were not fully accurate. Total granulocyte counts may be of clinical usefulness, but results obtained for other parameters were unreliable. 相似文献
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